Neuroactive steroids and their methods of use

ABSTRACT

Provided herein is a compound of Formula (I) or a pharmaceutically acceptable salt thereof, wherein R19, R5, R3a, R1a, R1b, R2a, R2b, R4a, R4b, R6a, R6b, R7a, R7b, R11a, R11b, R12a, R12b, R18, RD, and q are defined herein. L is selected from the group consisting of: wherein A indicates the point of attachment at C17 and wherein X is selected from the group consisting of —C(0)N(R55a)(R55b), —N(R55a)(R55b), —N(R55b)C(O)(R55a), and R55C wherein R55c is carbon-bound substituted or unsubstituted heteroaryl or substituted or unsubstituted aryl. Also provided herein are pharmaceutical compositions comprising a compound of Formula (I) and methods of using the compounds, e.g., in the treatment of CNS-related disorders.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional PatentApplication No. 62/775,470, filed Dec. 5, 2018, the entire contents ofwhich are incorporated by reference herein.

BACKGROUND OF THE INVENTION

Brain excitability is defined as the level of arousal of an animal, acontinuum that ranges from coma to convulsions, and is regulated byvarious neurotransmitters. In general, neurotransmitters are responsiblefor regulating the conductance of ions across neuronal membranes. Atrest, the neuronal membrane possesses a potential (or membrane voltage)of approximately −70 mV, the cell interior being negative with respectto the cell exterior. The potential (voltage) is the result of ion (K⁺,Na⁺, Cl⁻, organic anions) balance across the neuronal semipermeablemembrane. Neurotransmitters are stored in presynaptic vesicles and arereleased under the influence of neuronal action potentials. Whenreleased into the synaptic cleft, an excitatory chemical transmittersuch as acetylcholine will cause membrane depolarization (a change ofpotential occurs from −70 mV to −50 mV). This effect is mediated bypostsynaptic nicotinic receptors which are stimulated by acetylcholineto increase membrane permeability to Na⁺ ions. The reduced membranepotential stimulates neuronal excitability in the form of a postsynapticaction potential.

In the case of the GABA receptor complex (GRC), the effect on brainexcitability is mediated by γ-aminobutyric acid (GABA), aneurotransmitter. GABA has a profound influence on overall brainexcitability because up to 40% of the neurons in the brain utilize GABAas a neurotransmitter. GABA regulates the excitability of individualneurons by regulating the conductance of chloride ions across theneuronal membrane. GABA interacts with its recognition site on the GRCto facilitate the flow of chloride ions down an electrochemical gradientof the GRC into the cell. An intracellular increase in the levels ofthis anion causes hyperpolarization of the transmembrane potential,rendering the neuron less susceptible to excitatory inputs, i.e.,reduced neuron excitability. In other words, the higher the chloride ionconcentration in the neuron, the lower the brain excitability and levelof arousal.

It is well-documented that the GRC is responsible for the mediation ofanxiety, seizure activity, and sedation. Thus, GABA and drugs that actlike GABA or facilitate the effects of GABA (e.g., the therapeuticallyuseful barbiturates and benzodiazepines (BZs), such as Valium®) producetheir therapeutically useful effects by interacting with specificregulatory sites on the GRC. Accumulated evidence has now indicated thatin addition to the benzodiazepine and barbiturate binding site, the GRCcontains a distinct site for neuroactive steroids. See, e.g., Lan, N. C.et al., Neurochem. Res. (1991) 16:347-356.

Neuroactive steroids can occur endogenously. The most potent endogenousneuroactive steroids are 3α-hydroxy-5-reduced pregnan-20-one and3α-21-dihydroxy-5-reduced pregnan-20-one, metabolites of hormonalsteroids progesterone and deoxycorticosterone, respectively. The abilityof these steroid metabolites to alter brain excitability was recognizedin 1986 (Majewska, M. D. et al., Science 232:1004-1007 (1986); Harrison,N. L. et al., J Pharmacol. Exp. Ther. 241:346-353 (1987)).

New and improved compounds are needed that act as modulating agents forbrain excitability, as well as agents for the prevention and treatmentof CNS-related diseases. The compounds, compositions, and methodsdescribed herein are directed toward this end.

SUMMARY OF THE INVENTION

Provided herein are compounds designed to act as GABA receptormodulators. In some embodiments, such compounds are envisioned to beuseful as therapeutic agents for treating a CNS-related disorder.

In an aspect, provided herein is a compound of Formula (I):

or a pharmaceutically acceptable salt thereof;

-   -   wherein:    -   represents a single or double bond, provided if a double bond is        present, then one of R^(6a) or R^(6b) is absent and R⁵ is        absent;    -   L is selected from the group consisting of:

wherein A indicates the point of attachment at C17;

-   -   X is selected from the group consisting of        —C(O)N(R^(55a))(R^(55b)), —N(R^(55a))(R^(55b)),        —N(R^(55b))C(O)(R^(55a)), and R^(55c)—;    -   R^(Y) is each independently hydrogen, cyano, haloalkyl, or        unsubstituted alkyl;    -   R^(55c) is carbon-bound substituted or unsubstituted heteroaryl        or substituted or unsubstituted aryl;    -   R^(55a) and R^(55b) is each independently hydrogen, substituted        or unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, —OR^(A1), —N(R^(A1))₂—SR^(A1),        —C(═O)R^(A1), —C(═O)OR^(A1), —C(═O)SR^(A1), —C(═O)N(R^(A1))₂,        —OC(═O)R^(A1), —OC(═O)OR^(A1), —OC(═O)N(R^(A1))₂,        —OC(═O)SR^(A1), —OS(═O)₂R^(A1), —OS(═O)₂OR^(A1),        —OS(═O)₂N(R^(A1))₂, —N(R^(A1))C(═O)R^(A1),        —N(R^(A1))C(═NR^(A1))R^(A1), —N(R^(A1))C(═O)OR^(A1),        —N(R^(A1))C(═O)N(R^(A1))₂, —N(R^(A1))C(═NR^(A1)) N(R^(A1))₂,        —N(R^(A1))S(═O)₂R^(A1), —N(R^(A1))S(═O)₂OR^(A1),        —N(R^(A1))S(═O)₂N(R^(A1))₂, —SC(═O)R^(A1), —SC(═O)OR^(A1),        —SC(═O)SR^(A1), —SC(═O)N(R^(A1))₂, —S(═O)₂R^(A1),        —S(═O)₂OR^(A1), or —S(═O)₂N(R^(A1))₂, wherein each instance of        R^(A1) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, or substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, a sulfur protecting group when attached to sulfur, or        two R^(A1) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring;    -   or R^(55a) and R^(55b) may join together with the intervening        atoms to form a substituted or unsubstituted heterocyclyl or a        substituted or unsubstituted heteroaryl;    -   each of R^(1a), R^(1b), R^(2a), R^(2b), R^(4a), R^(4b), R^(7a),        R^(7b), R^(11a), R^(11b), R^(12a), and R^(12b) is independently        hydrogen, halogen, cyano, —NO₂, substituted or unsubstituted        alkyl, substituted or unsubstituted alkenyl, substituted or        unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,        substituted or unsubstituted heterocyclyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        —OR^(A1), —N(R^(A1))₂, —SR^(A1), —C(═O)R^(A1), —C(═O)OR^(A1),        —C(═O)SR^(A1), —C(═O)N(R^(A1))₂, —OC(═O)R^(A1), —OC(═O)OR^(A1),        —OC(═O)N(R^(A1))₂, —OC(═O)SR^(A1), —OS(═O)₂R^(A1),        —OS(═O)₂OR^(A1), —OS(═O)₂N(R^(A1))₂, —N(R^(A1))C(═O)R^(A1),        —N(R^(A1))C(═NR^(A1))R^(A1), —N(R^(A1))C(═O)OR^(A1),        —N(R^(A1))C(═O)N(R^(A1))₂, —N(R^(A1))C(═NR^(A1)) N(R^(A1))₂,        —N(R^(A1))S(═O)₂R^(A1), —N(R^(A1))S(═O)₂OR^(A1),        —N(R^(A1))S(═O)₂N(R^(A1))₂, —SC(═O)R^(A1), —SC(═O)OR^(A1),        —SC(═O)SR^(A1), —SC(═O)N(R^(A1))₂, —S(═O)₂R^(A1),        —S(═O)₂OR^(A1), or —S(═O)₂N(R^(A1))₂, wherein each instance of        R^(A1) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, or substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, or a sulfur protecting group when attached to sulfur;        or R^(11a) and R^(11b) are joined to form an oxo (═O) group;    -   R^(3a) is substituted or unsubstituted alkyl, substituted or        unsubstituted alkenyl, substituted or unsubstituted alkynyl,        substituted or unsubstituted carbocyclyl, substituted or        unsubstituted heterocyclyl, substituted or unsubstituted aryl,        or substituted or unsubstituted heteroaryl;    -   R⁵ is hydrogen or substituted or unsubstituted alkyl;    -   each of R^(6a) and R^(6b) is hydrogen, halogen, cyano, —NO₂,        —OH, substituted or unsubstituted alkyl, substituted or        unsubstituted alkenyl, or substituted or unsubstituted alkynyl;        or R^(6a) and R^(6b) are joined to form an oxo (═O) group;    -   R^(D) is independently hydrogen, halogen, —CN, —NO₂, oxo,        substituted or unsubstituted alkyl, substituted or unsubstituted        alkenyl, substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, —OR^(C3), —N(R^(C3))₂, —SR^(C3),        —C(═O)R^(C3), —C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂,        —OC(═O)R^(C3), —OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂,        —OC(═O)SR^(C3), —OS(═O)₂R^(C3), —OS(═O)₂OR^(C3),        —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),        —N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),        —N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,        —N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),        —N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),        —SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3),        —S(═O)₂OR^(C3), or —S(═O)₂N(R^(C3))₂, wherein each instance of        R^(C3) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        substituted or unsubstituted carbocyclyl, or substituted or        unsubstituted heterocyclyl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, or a sulfur protecting group when attached to sulfur;    -   R¹⁸ is substituted or unsubstituted alkyl;    -   R¹⁹ is hydrogen, substituted or unsubstituted alkyl, substituted        or unsubstituted alkenyl, or substituted or unsubstituted        alkynyl; and    -   q is an integer from 0 to 5;    -   provided that the compound is not:

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-I):

or a pharmaceutically acceptable salt thereof, wherein

-   -   each of R¹⁵, R^(15b), R^(16a), and R^(16b) is independently        hydrogen, halogen, —CN, —NO₂, substituted or unsubstituted        alkyl, substituted or unsubstituted alkenyl, substituted or        unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,        substituted or unsubstituted heterocyclyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        —OR^(C3), —N(R^(C3))₂, —SR^(C3), —C(═O)R^(C3), —C(═O)OR^(C3),        —C(═O)SR^(C3), —C(═O)N(R^(C3))₂, —OC(═O)R^(C3), —OC(═O)OR^(C3),        —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3), —OS(═O)₂R^(C3),        —OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),        —N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),        —N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,        —N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),        —N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),        —SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3),        —S(═O)₂OR^(C3), or —S(═O)₂N(R^(C3))₂, wherein each instance of        R^(C3) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        substituted or unsubstituted carbocyclyl, or substituted or        unsubstituted heterocyclyl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, a sulfur protecting group when attached to sulfur; or        R^(15a) and R^(15b) are joined to form an oxo (═O) group; or        R^(16a) and R^(16b) are joined to form an oxo (═O) group.

In some embodiments, the compound is a compound of Formula (I-a):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Ia):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-b), (I-c),(I-d), (I-e), (I-l), (I-m), (I-n), or (I-p):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-f), (I-g),or (I-h):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-i), (I-j),or (I-k):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-o):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-qq),(I-q), (I-s), (I-t), or (I-u):

or a pharmaceutically acceptable salt thereof, wherein

-   -   Q, Q′, and Q″ are each independently CR^(w) or N;    -   R^(w) is hydrogen, cyano, —NH₂, or substituted or unsubstituted        alkyl; and    -   at least one of Q, Q′, and Q″ is CR^(w).

In some embodiments, the compound is a compound of Formula (I-r):

or a pharmaceutically acceptable salt thereof, wherein

-   -   k is an integer 1 or 2;    -   R^(z) is substituted or unsubstituted alkyl or substituted or        unsubstituted aryl; or two R^(z)s on adjacent carbons combine        with the intervening atoms to form a substituted or        unsubstituted aryl; and    -   j is an integer 0-6.

In some embodiments, the compound is a compound of Formula (I-v), (I-w),or (I-x):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Ib),(I-Ic), (I-Id), (I-Ie), (I-Il), (I-Im), (I-In), (I-Ip1), or (I-Ip2):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-If),(I-Ig), or (I-Ih):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Ii),(I-Ij), or (I-Ik):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Io1) or(I-Io2):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Iqq),(I-Iq1), (I-Iq2), (I-It1), (I-It2), (I-Iu1), or (I-Iu2):

or a pharmaceutically acceptable salt thereof, wherein

-   -   Q, Q′, and Q″ are each independently CR^(w) or N;    -   R^(w) is hydrogen, cyano, —NH₂, or substituted or unsubstituted        alkyl; and    -   at least one of Q, Q′, and Q″ is CR^(w).

In some embodiments, the compound is a compound of Formula (I-Irr),(I-Ir1) or (I-Ir2):

or a pharmaceutically acceptable salt thereof, wherein

-   -   k is an integer 1 or 2;    -   R^(z) is substituted or unsubstituted alkyl or substituted or        unsubstituted aryl; or two R^(z)s on adjacent carbons combine        with the intervening atoms to form a substituted or        unsubstituted aryl; and    -   j an integer 0-6.

In some embodiments, the compound is a compound of Formula (I-Ir3) or(I-Ir4):

or a pharmaceutically acceptable salt thereof, wherein

-   -   k is an integer 1 or 2;    -   R^(z′) is substituted or unsubstituted alkyl or cyano; and    -   j′ an integer 0-4.

In some embodiments, the compound is a compound of Formula (I-Iw1),(I-Iw2), (I-Ix1), or (I-Ix2):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is selected from the group consistingof the compounds identified in Table 1 herein.

Compounds of the present invention as described herein, act, in certainembodiments, as GABA_(A) receptor modulators. In certain embodiments,the compounds described herein can act as positive allosteric modulatorsof the GABA receptor e.g., of the GABA_(A) receptor.

In one embodiment, the compounds described herein (e.g., a compound ofFormula I or Table 1) exhibit higher selectivity for modulation of theα4β3δ configuration of GABA_(A) receptor relative to the α1β2γ2configuration of GABA_(A) receptor.

In an aspect, provided herein is a pharmaceutical composition comprisinga compound described herein (e.g., a compound of Formula (I)), or apharmaceutically acceptable salt thereof, and a pharmaceuticallyacceptable excipient. In certain embodiments, the compound of thepresent invention is provided in an effective amount in thepharmaceutical composition. In certain embodiments, the compound of thepresent invention is provided in a therapeutically effective amount. Incertain embodiments, the compound of the present invention is providedin a prophylactically effective amount.

In an aspect, provided herein is a pharmaceutically acceptable salt of acompound described herein (e.g., a compound of Formula (I)).

In certain embodiments, the compound is administered orally,subcutaneously, intravenously, or intramuscularly. In certainembodiments, the compound is administered orally. In certainembodiments, the compound is administered chronically. In certainembodiments, the compound is administered continuously, e.g., bycontinuous intravenous infusion.

Compounds of the present invention as described herein, act, in certainembodiments, as GABA receptor modulators, e.g., effecting the GABA_(A)receptor in either a positive or negative manner. As modulators of theexcitability of the central nervous system (CNS), as mediated by theirability to modulate GABA_(A) receptor, such compounds are expected tohave CNS-activity.

In an aspect, described herein is a method of treating a CNS-relateddisorder in a subject in need thereof, comprising administering to thesubject an effective amount of a compound described herein or apharmaceutically acceptable salt thereof.

In some embodiments, the CNS-related disorder is a sleep disorder, amood disorder, a schizophrenia spectrum disorder, a convulsive disorder,a disorder of memory and/or cognition, a movement disorder, apersonality disorder, autism spectrum disorder, pain, traumatic braininjury, a vascular disease, a substance abuse disorder and/or withdrawalsyndrome, tinnitus, or status epilepticus.

In some embodiments, the CNS-related disorder is depression. In someembodiments, the CNS-related disorder is postpartum depression. In someembodiments, the CNS-related disorder is major depressive disorder. Insome embodiments, the major depressive disorder is moderate majordepressive disorder. In some embodiments, the major depressive disorderis severe major depressive disorder.

DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS OF THE INVENTION

As generally described herein, the present invention provides compoundsdesigned, for example, to act as GABA receptor modulators. In certainembodiments, such compounds are envisioned to be useful as therapeuticagents for treating a CNS-related disorder (e.g., a disorder asdescribed herein, for example depression, such as post-partum depressionor major depressive disorder).

Definitions Chemical Definitions

Definitions of specific functional groups and chemical terms aredescribed in more detail below. The chemical elements are identified inaccordance with the Periodic Table of the Elements, CAS version,Handbook of Chemistry and Physics, 75^(th) Ed., inside cover, andspecific functional groups are generally defined as described therein.Additionally, general principles of organic chemistry, as well asspecific functional moieties and reactivity, are described in ThomasSorrell, Organic Chemistry, University Science Books, Sausalito, 1999;Smith and March, March's Advanced Organic Chemistry, 5^(th) Edition,John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive OrganicTransformations, VCH Publishers, Inc., New York, 1989; and Carruthers,Some Modern Methods of Organic Synthesis, 3^(rd) Edition, CambridgeUniversity Press, Cambridge, 1987.

Isomers, e.g., stereoisomers, can be isolated from mixtures by methodsknown to those skilled in the art, including chiral high pressure liquidchromatography (HPLC) and the formation and crystallization of chiralsalts; or preferred isomers can be prepared by asymmetric syntheses.See, for example, Jacques et al., Enantiomers, Racemates and Resolutions(Wiley Interscience, New York, 1981); Wilen et al., Tetrahedron 33:2725(1977); Eliel, Stereochemistry of Carbon Compounds (McGraw-Hill, N Y,1962); and Wilen, Tables of Resolving Agents and Optical Resolutions p.268 (E. L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, Ind.1972). The invention additionally encompasses compounds described hereinas individual isomers substantially free of other isomers, andalternatively, as mixtures of various isomers.

“Stereoisomers”: It is also to be understood that compounds that havethe same molecular formula but differ in the nature or sequence ofbonding of their atoms or the arrangement of their atoms in space aretermed “isomers.” Isomers that differ in the arrangement of their atomsin space are termed “stereoisomers.” Stereoisomers that are not mirrorimages of one another are termed “diastereomers” and those that arenon-superimposable mirror images of each other are termed “enantiomers.”When a compound has an asymmetric center, for example, it is bonded tofour different groups, a pair of enantiomers is possible. An enantiomercan be characterized by the absolute configuration of its asymmetriccenter and is described by the R- and S-sequencing rules of Cahn andPrelog, or by the manner in which the molecule rotates the plane ofpolarized light and designated as dextrorotatory or levorotatory (i.e.,as (+) or (−)-isomers respectively). A chiral compound can exist aseither individual enantiomer or as a mixture thereof. A mixturecontaining equal proportions of the enantiomers is called a “racemicmixture”.

As used herein, a pure enantiomeric compound is substantially free fromother enantiomers or stereoisomers of the compound (i.e., inenantiomeric excess). In other words, an “S” form of the compound issubstantially free from the “R” form of the compound and is, thus, inenantiomeric excess of the “R” form. The term “enantiomerically pure” or“pure enantiomer” denotes that the compound comprises more than 75% byweight, more than 80% by weight, more than 85% by weight, more than 90%by weight, more than 91% by weight, more than 92% by weight, more than93% by weight, more than 94% by weight, more than 95% by weight, morethan 96% by weight, more than 97% by weight, more than 98% by weight,more than 98.5% by weight, more than 99% by weight, more than 99.2% byweight, more than 99.5% by weight, more than 99.6% by weight, more than99.7% by weight, more than 99.8% by weight or more than 99.9% by weight,of the enantiomer. In certain embodiments, the weights are based upontotal weight of all enantiomers or stereoisomers of the compound.

As used herein, the term “diastereomeric purity” refers to the amount ofa compound having the depicted absolute stereochemistry, expressed as apercentage of the total amount of the depicted compound and itsdiastereomers. The term “diastereomierically pure” denotes that thecompound comprises more than 75% by weight, more than 80% by weight,more than 85% by weight, more than 90% by weight, more than 91% byweight, more than 92% by weight, more than 93% by weight, more than 94%by weight, more than 95% by weight, more than 96% by weight, more than97% by weight, more than 98% by weight, more than 98.5% by weight, morethan 99% by weight, more than 99.2% by weight, more than 99.5% byweight, more than 99.6% by weight, more than 99.7% by weight, more than99.8% by weight or more than 99.9% by weight, of the diastereomer.Methods for determining diastereomeric and enantiomeric purity arewell-known in the art. Diastereomeric purity can be determined by anyanalytical method capable of quantitatively distinguishing between acompound and its diastereomers, such as high performance liquidchromatography (HPLC).

In the compositions provided herein, an enantiomerically pure compoundcan be present with other active or inactive ingredients. For example, apharmaceutical composition comprising enantiomerically pureR-position/center/carbon compound can comprise, for example, about 90%excipient and about 10% enantiomerically pure R-compound. In certainembodiments, the enantiomerically pure R-compound in such compositionscan, for example, comprise, at least about 95% by weight R-compound andat most about 5% by weight S-compound, by total weight of the compound.For example, a pharmaceutical composition comprising enantiomericallypure S-compound can comprise, for example, about 90% excipient and about10% enantiomerically pure S-compound. In certain embodiments, theenantiomerically pure S-compound in such compositions can, for example,comprise, at least about 95% by weight S-compound and at most about 5%by weight R-compound, by total weight of the compound. In certainembodiments, the active ingredient can be formulated with little or noexcipient or carrier.

The articles “a” and “an” may be used herein to refer to one or to morethan one (i.e. at least one) of the grammatical objects of the article.By way of example “an analogue” means one analogue or more than oneanalogue.

When a range of values is listed, it is intended to encompass each valueand sub-range within the range. For example “C₁₋₆alkyl” is intended toencompass, C₁, C₂, C₃, C₄, C₅, C₆, C₁₋₆, C₁₋₅, C₁₋₄, C₁₋₃, C₁₋₂, C₂₋₆,C₂₋₅, C₂₋₄, C₂₋₃, C₃₋₆, C₃₋₅, C₃₋₄, C₄₋₆, C₄₋₅, and C₅₋₆ alkyl.

The following terms are intended to have the meanings presentedtherewith below and are useful in understanding the description andintended scope of the present invention.

“Alkyl” refers to a radical of a straight-chain or branched saturatedhydrocarbon group having from 1 to 20 carbon atoms (“C₁₋₂₀ alkyl”). Insome embodiments, an alkyl group has 1 to 12 carbon atoms (“C₁₋₁₂alkyl”). In some embodiments, an alkyl group has 1 to 10 carbon atoms(“C₁₋₁₀ alkyl”). In some embodiments, an alkyl group has 1 to 9 carbonatoms (“C₁₋₉ alkyl”). In some embodiments, an alkyl group has 1 to 8carbon atoms (“C₁₋₈ alkyl”). In some embodiments, an alkyl group has 1to 7 carbon atoms (“C₁₋₇ alkyl”). In some embodiments, an alkyl grouphas 1 to 6 carbon atoms (“C₁₋₆ alkyl”, also referred to herein as “loweralkyl”). In some embodiments, an alkyl group has 1 to 5 carbon atoms(“C₁₋₅ alkyl”). In some embodiments, an alkyl group has 1 to 4 carbonatoms (“C₁₋₄ alkyl”). In some embodiments, an alkyl group has 1 to 3carbon atoms (“C₁₋₃ alkyl”). In some embodiments, an alkyl group has 1to 2 carbon atoms (“C₁₋₂ alkyl”). In some embodiments, an alkyl grouphas 1 carbon atom (“C₁ alkyl”). In some embodiments, an alkyl group has2 to 6 carbon atoms (“C₂₋₆ alkyl”). Examples of C₁₋₆ alkyl groupsinclude methyl (C₁), ethyl (C₂), n-propyl (C₃), isopropyl (C₃), n-butyl(C₄), tert-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄), n-pentyl (C₅),3-pentanyl (C₅), amyl (C₅), neopentyl (C₅), 3-methyl-2-butanyl (C₅),tertiary amyl (C₅), and n-hexyl (C₆). Additional examples of alkylgroups include n-heptyl (C₇), n-octyl (C₈) and the like. Unlessotherwise specified, each instance of an alkyl group is independentlyoptionally substituted, i.e., unsubstituted (an “unsubstituted alkyl”)or substituted (a “substituted alkyl”) with one or more substituents;e.g., for instance from 1 to 5 substituents, 1 to 3 substituents, or 1substituent. In certain embodiments, the alkyl group is unsubstitutedC₁₋₁₀ alkyl (e.g., —CH₃). In certain embodiments, the alkyl group issubstituted C₁₋₁₀ alkyl. Common alkyl abbreviations include Me (—CH₃),Et (—CH₂CH₃), iPr (—CH(CH₃)₂), nPr (—CH₂CH₂CH₃), n-Bu (—CH₂CH₂CH₂CH₃),or i-Bu (—CH₂CH(CH₃)₂).

“Alkylene” refers to an alkyl group wherein two hydrogens are removed toprovide a divalent radical, and which may be substituted orunsubstituted. Unsubstituted alkylene groups include, but are notlimited to, methylene (—CH₂—), ethylene (—CH₂CH₂—), propylene(—CH₂CH₂CH₂—), butylene (—CH₂CH₂CH₂CH₂—), pentylene (—CH₂CH₂CH₂CH₂CH₂—),hexylene (—CH₂CH₂CH₂CH₂CH₂CH₂—), and the like. Exemplary substitutedalkylene groups, e.g., substituted with one or more alkyl (methyl)groups, include but are not limited to, substituted methylene(—CH(CH₃)—, (—C(CH₃)₂—), substituted ethylene (—CH(CH₃)CH₂—,—CH₂CH(CH₃)—, —C(CH₃)₂CH₂—, —CH₂C(CH₃)₂—), substituted propylene(—CH(CH₃)CH₂CH₂—, —CH₂CH(CH₃)CH₂—, —CH₂CH₂CH(CH₃)—, —C(CH₃)₂CH₂CH₂—,—CH₂C(CH₃)₂CH₂—, —CH₂CH₂C(CH₃)₂—), and the like. When a range or numberof carbons is provided for a particular alkylene group, it is understoodthat the range or number refers to the range or number of carbons in thelinear carbon divalent chain. Alkylene groups may be substituted orunsubstituted with one or more substituents as described herein.

“Alkenyl” refers to a radical of a straight-chain or branchedhydrocarbon group having from 2 to 20 carbon atoms, one or morecarbon-carbon double bonds (e.g., 1, 2, 3, or 4 carbon-carbon doublebonds), and optionally one or more carbon-carbon triple bonds (e.g., 1,2, 3, or 4 carbon-carbon triple bonds) (“C₂₋₂₀ alkenyl”). In certainembodiments, alkenyl does not contain any triple bonds. In someembodiments, an alkenyl group has 2 to 10 carbon atoms (“C₂₋₁₀alkenyl”). In some embodiments, an alkenyl group has 2 to 9 carbon atoms(“C₂₋₉ alkenyl”). In some embodiments, an alkenyl group has 2 to 8carbon atoms (“C₂₋₈ alkenyl”). In some embodiments, an alkenyl group has2 to 7 carbon atoms (“C₂₋₇ alkenyl”). In some embodiments, an alkenylgroup has 2 to 6 carbon atoms (“C₂₋₆ alkenyl”). In some embodiments, analkenyl group has 2 to 5 carbon atoms (“C₂₋₅ alkenyl”). In someembodiments, an alkenyl group has 2 to 4 carbon atoms (“C₂₋₄ alkenyl”).In some embodiments, an alkenyl group has 2 to 3 carbon atoms (“C₂₋₃alkenyl”). In some embodiments, an alkenyl group has 2 carbon atoms (“C₂alkenyl”). The one or more carbon-carbon double bonds can be internal(such as in 2-butenyl) or terminal (such as in 1-butenyl). Examples ofC₂₋₄ alkenyl groups include ethenyl (C₂), 1-propenyl (C₃), 2-propenyl(C₃), 1-butenyl (C₄), 2-butenyl (C₄), butadienyl (C₄), and the like.Examples of C₂₋₆ alkenyl groups include the aforementioned C₂₋₄ alkenylgroups as well as pentenyl (C₅), pentadienyl (C₅), hexenyl (C), and thelike. Additional examples of alkenyl include heptenyl (C₇), octenyl(C₈), octatrienyl (C₈), and the like. Unless otherwise specified, eachinstance of an alkenyl group is independently optionally substituted,i.e., unsubstituted (an “unsubstituted alkenyl”) or substituted (a“substituted alkenyl”) with one or more substituents e.g., for instancefrom 1 to 5 substituents, 1 to 3 substituents, or 1 substituent. Incertain embodiments, the alkenyl group is unsubstituted C₂₋₁₀ alkenyl.In certain embodiments, the alkenyl group is substituted C₂₋₁₀ alkenyl.

“Alkynyl” refers to a radical of a straight-chain or branchedhydrocarbon group having from 2 to 20 carbon atoms, one or morecarbon-carbon triple bonds (e.g., 1, 2, 3, or 4 carbon-carbon triplebonds), and optionally one or more carbon-carbon double bonds (e.g., 1,2, 3, or 4 carbon-carbon double bonds) (“C₂₋₂₀ alkynyl”). In certainembodiments, alkynyl does not contain any double bonds. In someembodiments, an alkynyl group has 2 to 10 carbon atoms (“C₂₋₁₀alkynyl”). In some embodiments, an alkynyl group has 2 to 9 carbon atoms(“C₂₋₉ alkynyl”). In some embodiments, an alkynyl group has 2 to 8carbon atoms (“C₂₋₈ alkynyl”). In some embodiments, an alkynyl group has2 to 7 carbon atoms (“C₂₋₇ alkynyl”). In some embodiments, an alkynylgroup has 2 to 6 carbon atoms (“C₂₋₆ alkynyl”). In some embodiments, analkynyl group has 2 to 5 carbon atoms (“C_(2-s) alkynyl”). In someembodiments, an alkynyl group has 2 to 4 carbon atoms (“C₂₋₄ alkynyl”).In some embodiments, an alkynyl group has 2 to 3 carbon atoms (“C₂₋₃alkynyl”). In some embodiments, an alkynyl group has 2 carbon atoms (“C₂alkynyl”). The one or more carbon-carbon triple bonds can be internal(such as in 2-butynyl) or terminal (such as in 1-butynyl). Examples ofC₂₋₄ alkynyl groups include, without limitation, ethynyl (C₂),1-propynyl (C₃), 2-propynyl (C₃), 1-butynyl (C₄), 2-butynyl (C₄), andthe like. Examples of C₂₋₆ alkenyl groups include the aforementionedC₂₋₄ alkynyl groups as well as pentynyl (C₅), hexynyl (C), and the like.Additional examples of alkynyl include heptynyl (C₇), octynyl (C₈), andthe like. Unless otherwise specified, each instance of an alkynyl groupis independently optionally substituted, i.e., unsubstituted (an“unsubstituted alkynyl”) or substituted (a “substituted alkynyl”) withone or more substituents; e.g., for instance from 1 to 5 substituents, 1to 3 substituents, or 1 substituent. In certain embodiments, the alkynylgroup is unsubstituted C₂₋₁₀ alkynyl. In certain embodiments, thealkynyl group is substituted C₂₋₁₀ alkynyl.

The term “heteroalkyl,” as used herein, refers to an alkyl group, asdefined herein, which further comprises 1 or more (e.g., 1, 2, 3, or 4)heteroatoms (e.g., oxygen, sulfur, nitrogen, boron, silicon, phosphorus)within the parent chain, wherein the one or more heteroatoms is insertedbetween adjacent carbon atoms within the parent carbon chain and/or oneor more heteroatoms is inserted between a carbon atom and the parentmolecule, i.e., between the point of attachment. In certain embodiments,a heteroalkyl group refers to a saturated group having from 1 to 10carbon atoms and 1, 2, 3, or 4 heteroatoms (“heteroC₁₋₁₀ alkyl”). Insome embodiments, a heteroalkyl group is a saturated group having 1 to 9carbon atoms and 1, 2, 3, or 4 heteroatoms (“heteroC₁₋₉ alkyl”). In someembodiments, a heteroalkyl group is a saturated group having 1 to 8carbon atoms and 1, 2, 3, or 4 heteroatoms (“heteroC₁₋₈ alkyl”). In someembodiments, a heteroalkyl group is a saturated group having 1 to 7carbon atoms and 1, 2, 3, or 4 heteroatoms (“heteroC₁₋₇ alkyl”). In someembodiments, a heteroalkyl group is a group having 1 to 6 carbon atomsand 1, 2, or 3 heteroatoms (“heteroC₁₋₆ alkyl”). In some embodiments, aheteroalkyl group is a saturated group having 1 to 5 carbon atoms and 1or 2 heteroatoms (“heteroC₁₋₅ alkyl”). In some embodiments, aheteroalkyl group is a saturated group having 1 to 4 carbon atoms and 1or 2 heteroatoms (“heteroC₁₋₄ alkyl”). In some embodiments, aheteroalkyl group is a saturated group having 1 to 3 carbon atoms and 1heteroatom (“heteroC₁₋₃ alkyl”). In some embodiments, a heteroalkylgroup is a saturated group having 1 to 2 carbon atoms and 1 heteroatom(“heteroC₁₋₂ alkyl”). In some embodiments, a heteroalkyl group is asaturated group having 1 carbon atom and 1 heteroatom (“heteroC₁alkyl”). In some embodiments, a heteroalkyl group is a saturated grouphaving 2 to 6 carbon atoms and 1 or 2 heteroatoms (“heteroC₂₋₆ alkyl”).Unless otherwise specified, each instance of a heteroalkyl group isindependently unsubstituted (an “unsubstituted heteroalkyl”) orsubstituted (a “substituted heteroalkyl”) with one or more substituents.In certain embodiments, the heteroalkyl group is an unsubstitutedheteroC₁₋₁₀ alkyl. In certain embodiments, the heteroalkyl group is asubstituted heteroC₁₋₁₀ alkyl.

“Aryl” refers to a radical of a monocyclic or polycyclic (e.g., bicyclicor tricyclic) 4n+2 aromatic ring system (e.g., having 6, 10, or 14 πelectrons shared in a cyclic array) having 6-14 ring carbon atoms andzero heteroatoms provided in the aromatic ring system (“C₆₋₁₄ aryl”). Insome embodiments, an aryl group has six ring carbon atoms (“C₆ aryl”;e.g., phenyl). In some embodiments, an aryl group has ten ring carbonatoms (“C₁₀ aryl”; e.g., naphthyl such as 1-naphthyl and 2-naphthyl). Insome embodiments, an aryl group has fourteen ring carbon atoms (“C₁₄aryl”; e.g., anthracyl). “Aryl” also includes ring systems wherein thearyl ring, as defined above, is fused with one or more carbocyclyl orheterocyclyl groups wherein the radical or point of attachment is on thearyl ring, and in such instances, the number of carbon atoms continue todesignate the number of carbon atoms in the aryl ring system. Typicalaryl groups include, but are not limited to, groups derived fromaceanthrylene, acenaphthylene, acephenanthrylene, anthracene, azulene,benzene, chrysene, coronene, fluoranthene, fluorene, hexacene,hexaphene, hexalene, as-indacene, s-indacene, indane, indene,naphthalene, octacene, octaphene, octalene, ovalene, penta-2,4-diene,pentacene, pentalene, pentaphene, perylene, phenalene, phenanthrene,picene, pleiadene, pyrene, pyranthrene, rubicene, triphenylene, andtrinaphthalene. Particularly aryl groups include phenyl, naphthyl,indenyl, and tetrahydronaphthyl. Unless otherwise specified, eachinstance of an aryl group is independently optionally substituted, i.e.,unsubstituted (an “unsubstituted aryl”) or substituted (a “substitutedaryl”) with one or more substituents. In certain embodiments, the arylgroup is unsubstituted C₆₋₁₄ aryl. In certain embodiments, the arylgroup is substituted C₆₋₁₄ aryl.

In certain embodiments, an aryl group is substituted with one or more ofgroups selected from halogen, C₁-C₈ alkyl, C₁-C₈ haloalkyl, cyano,hydroxy, C₁-C₈ alkoxy, and amino.

Examples of representative substituted aryls include the following

wherein one of R⁵⁶ and R⁵⁷ may be hydrogen and at least one of R⁵⁶ andR⁵⁷ is each independently selected from C₁-C₈ alkyl, C₁-C₈ haloalkyl,4-10 membered heterocyclyl, alkanoyl, C₁-C₈ alkoxy, heteroaryloxy,alkylamino, arylamino, heteroarylamino, NR⁵⁸COR⁵⁹, NR⁵⁸SOR⁵⁹ NR⁵⁸SO₂R⁵⁹,COOalkyl, COOaryl, CONR⁵⁸R⁵⁹, CONR⁵⁸OR⁵⁹, NR⁵⁸R⁵⁹, SO₂NR⁵⁸R⁵⁹, S-alkyl,SOalkyl, SO₂alkyl, Saryl, SOaryl, SO₂aryl; or R⁵⁶ and R⁵⁷ may be joinedto form a cyclic ring (saturated or unsaturated) from 5 to 8 atoms,optionally containing one or more heteroatoms selected from the group N,O, or S. R⁶⁰ and R⁶¹ are independently hydrogen, C₁-C₈ alkyl,C₁-C₄haloalkyl, C₃-C₁₀ cycloalkyl, 4-10 membered heterocyclyl, C₆-C₁₀aryl, substituted C₆-C₁₀ aryl, 5-10 membered heteroaryl, or substituted5-10 membered heteroaryl.

“Fused aryl” refers to an aryl having two of its ring carbon in commonwith a second aryl or heteroaryl ring or with a carbocyclyl orheterocyclyl ring.

“Heteroaryl” refers to a radical of a 5-10 membered monocyclic orbicyclic 4n+2 aromatic ring system (e.g., having 6 or 10 n electronsshared in a cyclic array) having ring carbon atoms and 1-4 ringheteroatoms provided in the aromatic ring system, wherein eachheteroatom is independently selected from nitrogen, oxygen and sulfur(“5-10 membered heteroaryl”). In heteroaryl groups that contain one ormore nitrogen atoms, the point of attachment can be a carbon or nitrogenatom, as valency permits. Heteroaryl bicyclic ring systems can includeone or more heteroatoms in one or both rings. “Heteroaryl” includes ringsystems wherein the heteroaryl ring, as defined above, is fused with oneor more carbocyclyl or heterocyclyl groups wherein the point ofattachment is on the heteroaryl ring, and in such instances, the numberof ring members continue to designate the number of ring members in theheteroaryl ring system. “Heteroaryl” also includes ring systems whereinthe heteroaryl ring, as defined above, is fused with one or more arylgroups wherein the point of attachment is either on the aryl orheteroaryl ring, and in such instances, the number of ring membersdesignates the number of ring members in the fused (aryl/heteroaryl)ring system. Bicyclic heteroaryl groups wherein one ring does notcontain a heteroatom (e.g., indolyl, quinolinyl, carbazolyl, and thelike) the point of attachment can be on either ring, i.e., either thering bearing a heteroatom (e.g., 2-indolyl) or the ring that does notcontain a heteroatom (e.g., 5-indolyl).

In some embodiments, a heteroaryl group is a 5-10 membered aromatic ringsystem having ring carbon atoms and 1-4 ring heteroatoms provided in thearomatic ring system, wherein each heteroatom is independently selectedfrom nitrogen, oxygen, and sulfur (“5-10 membered heteroaryl”). In someembodiments, a heteroaryl group is a 5-8 membered aromatic ring systemhaving ring carbon atoms and 1-4 ring heteroatoms provided in thearomatic ring system, wherein each heteroatom is independently selectedfrom nitrogen, oxygen, and sulfur (“5-8 membered heteroaryl”). In someembodiments, a heteroaryl group is a 5-6 membered aromatic ring systemhaving ring carbon atoms and 1-4 ring heteroatoms provided in thearomatic ring system, wherein each heteroatom is independently selectedfrom nitrogen, oxygen, and sulfur (“5-6 membered heteroaryl”). In someembodiments, the 5-6 membered heteroaryl has 1-3 ring heteroatomsselected from nitrogen, oxygen, and sulfur. In some embodiments, the 5-6membered heteroaryl has 1-2 ring heteroatoms selected from nitrogen,oxygen, and sulfur. In some embodiments, the 5-6 membered heteroaryl has1 ring heteroatom selected from nitrogen, oxygen, and sulfur. Unlessotherwise specified, each instance of a heteroaryl group isindependently optionally substituted, i.e., unsubstituted (an“unsubstituted heteroaryl”) or substituted (a “substituted heteroaryl”)with one or more substituents. In certain embodiments, the heteroarylgroup is unsubstituted 5-14 membered heteroaryl. In certain embodiments,the heteroaryl group is substituted 5-14 membered heteroaryl.

Exemplary 5-membered heteroaryl groups containing one heteroatominclude, without limitation, pyrrolyl, furanyl and thiophenyl. Exemplary5-membered heteroaryl groups containing two heteroatoms include, withoutlimitation, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, andisothiazolyl. Exemplary 5-membered heteroaryl groups containing threeheteroatoms include, without limitation, triazolyl, oxadiazolyl, andthiadiazolyl. Exemplary 5-membered heteroaryl groups containing fourheteroatoms include, without limitation, tetrazolyl. Exemplary6-membered heteroaryl groups containing one heteroatom include, withoutlimitation, pyridinyl. Exemplary 6-membered heteroaryl groups containingtwo heteroatoms include, without limitation, pyridazinyl, pyrimidinyl,and pyrazinyl. Exemplary 6-membered heteroaryl groups containing threeor four heteroatoms include, without limitation, triazinyl andtetrazinyl, respectively. Exemplary 7-membered heteroaryl groupscontaining one heteroatom include, without limitation, azepinyl,oxepinyl, and thiepinyl. Exemplary 5,6-bicyclic heteroaryl groupsinclude, without limitation, indolyl, isoindolyl, indazolyl,benzotriazolyl, benzothiophenyl, isobenzothiophenyl, benzofuranyl,benzoisofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl,benzoxadiazolyl, benzthiazolyl, benzisothiazolyl, benzthiadiazolyl,indolizinyl, and purinyl. Exemplary 6,6-bicyclic heteroaryl groupsinclude, without limitation, naphthyridinyl, pteridinyl, quinolinyl,isoquinolinyl, cinnolinyl, quinoxalinyl, phthalazinyl, and quinazolinyl.

Examples of representative heteroaryls include the following:

wherein each Z is selected from carbonyl, N, NR⁶⁵, O, and S; and R⁶⁵ isindependently hydrogen, C₁-C₈ alkyl, C₃-C₁₀ cycloalkyl, 4-10 memberedheterocyclyl, C₆-C₁₀ aryl, and 5-10 membered heteroaryl.

“Carbocyclyl” or “carbocyclic” refers to a radical of a non-aromaticcyclic hydrocarbon group having from 3 to 10 ring carbon atoms (“C₃₋₁₀carbocyclyl”) and zero heteroatoms in the non-aromatic ring system. Insome embodiments, a carbocyclyl group has 3 to 8 ring carbon atoms(“C₃₋₈ carbocyclyl”). In some embodiments, a carbocyclyl group has 3 to6 ring carbon atoms (“C₃₋₆ carbocyclyl”). In some embodiments, acarbocyclyl group has 3 to 6 ring carbon atoms (“C₃₋₆ carbocyclyl”). Insome embodiments, a carbocyclyl group has 5 to 10 ring carbon atoms(“C₅₋₁₀ carbocyclyl”). Exemplary C₃₋₆ carbocyclyl groups include,without limitation, cyclopropyl (C₃), cyclopropenyl (C₃), cyclobutyl(C₄), cyclobutenyl (C₄), cyclopentyl (C₅), cyclopentenyl (C₅),cyclohexyl (C₆), cyclohexenyl (C₆), cyclohexadienyl (C₆), and the like.Exemplary C₃₋₈ carbocyclyl groups include, without limitation, theaforementioned C₃₋₆ carbocyclyl groups as well as cycloheptyl (C₇),cycloheptenyl (C₇), cycloheptadienyl (C₇), cycloheptatrienyl (C₇),cyclooctyl (C₈), cyclooctenyl (C₈), bicyclo[2.2.1]heptanyl (C₇),bicyclo[2.2.2]octanyl (C₈), and the like. Exemplary C₃₋₁₀ carbocyclylgroups include, without limitation, the aforementioned C₃₋₈ carbocyclylgroups as well as cyclononyl (C₉), cyclononenyl (C₉), cyclodecyl (C₁₀),cyclodecenyl (C₁₀), octahydro-1H-indenyl (C₉), decahydronaphthalenyl(C₁₀), spiro[4.5]decanyl (C₁₀), and the like. As the foregoing examplesillustrate, in certain embodiments, the carbocyclyl group is eithermonocyclic (“monocyclic carbocyclyl”) or contain a fused, bridged orspiro ring system such as a bicyclic system (“bicyclic carbocyclyl”) andcan be saturated or can be partially unsaturated. “Carbocyclyl” alsoincludes ring systems wherein the carbocyclyl ring, as defined above, isfused with one or more aryl or heteroaryl groups wherein the point ofattachment is on the carbocyclyl ring, and in such instances, the numberof carbons continue to designate the number of carbons in thecarbocyclic ring system. Unless otherwise specified, each instance of acarbocyclyl group is independently optionally substituted, i.e.,unsubstituted (an “unsubstituted carbocyclyl”) or substituted (a“substituted carbocyclyl”) with one or more substituents. In certainembodiments, the carbocyclyl group is unsubstituted C₃₋₁₀ carbocyclyl.In certain embodiments, the carbocyclyl group is a substituted C₃₋₁₀carbocyclyl.

In some embodiments, “carbocyclyl” is a monocyclic, saturatedcarbocyclyl group having from 3 to 10 ring carbon atoms (“C₃₋₁₀cycloalkyl”). In some embodiments, a cycloalkyl group has 3 to 8 ringcarbon atoms (“C₃₋₈ cycloalkyl”). In some embodiments, a cycloalkylgroup has 3 to 6 ring carbon atoms (“C₃₋₆ cycloalkyl”). In someembodiments, a cycloalkyl group has 5 to 6 ring carbon atoms (“C₅₋₆cycloalkyl”). In some embodiments, a cycloalkyl group has 5 to 10 ringcarbon atoms (“C₅₋₁₀ cycloalkyl”). Examples of C₅₋₆ cycloalkyl groupsinclude cyclopentyl (C₅) and cyclohexyl (C₅). Examples of C₃₋₆cycloalkyl groups include the aforementioned C₅₋₆ cycloalkyl groups aswell as cyclopropyl (C₃) and cyclobutyl (C₄). Examples of C₃₋₈cycloalkyl groups include the aforementioned C₃₋₆ cycloalkyl groups aswell as cycloheptyl (C₇) and cyclooctyl (C₈). Unless otherwisespecified, each instance of a cycloalkyl group is independentlyunsubstituted (an “unsubstituted cycloalkyl”) or substituted (a“substituted cycloalkyl”) with one or more substituents. In certainembodiments, the cycloalkyl group is unsubstituted C₃₋₁₀ cycloalkyl. Incertain embodiments, the cycloalkyl group is substituted C₃₋₁₀cycloalkyl.

“Heterocyclyl” or “heterocyclic” refers to a radical of a 3- to10-membered non-aromatic ring system having ring carbon atoms and 1 to 4ring heteroatoms, wherein each heteroatom is independently selected fromnitrogen, oxygen, sulfur, boron, phosphorus, and silicon (“3-10 memberedheterocyclyl”). In heterocyclyl groups that contain one or more nitrogenatoms, the point of attachment can be a carbon or nitrogen atom, asvalency permits. A heterocyclyl group can either be monocyclic(“monocyclic heterocyclyl”) or a fused, bridged or spiro ring systemsuch as a bicyclic system (“bicyclic heterocyclyl”), and can besaturated or can be partially unsaturated. Heterocyclyl bicyclic ringsystems can include one or more heteroatoms in one or both rings.“Heterocyclyl” also includes ring systems wherein the heterocyclyl ring,as defined above, is fused with one or more carbocyclyl groups whereinthe point of attachment is either on the carbocyclyl or heterocyclylring, or ring systems wherein the heterocyclyl ring, as defined above,is fused with one or more aryl or heteroaryl groups, wherein the pointof attachment is on the heterocyclyl ring, and in such instances, thenumber of ring members continue to designate the number of ring membersin the heterocyclyl ring system. Unless otherwise specified, eachinstance of heterocyclyl is independently optionally substituted, i.e.,unsubstituted (an “unsubstituted heterocyclyl”) or substituted (a“substituted heterocyclyl”) with one or more substituents. In certainembodiments, the heterocyclyl group is unsubstituted 3-10 memberedheterocyclyl. In certain embodiments, the heterocyclyl group issubstituted 3-10 membered heterocyclyl.

In some embodiments, a heterocyclyl group is a 5-10 memberednon-aromatic ring system having ring carbon atoms and 1-4 ringheteroatoms, wherein each heteroatom is independently selected fromnitrogen, oxygen, sulfur, boron, phosphorus, and silicon (“5-10 memberedheterocyclyl”). In some embodiments, a heterocyclyl group is a 5-8membered non-aromatic ring system having ring carbon atoms and 1-4 ringheteroatoms, wherein each heteroatom is independently selected fromnitrogen, oxygen, and sulfur (“5-8 membered heterocyclyl”). In someembodiments, a heterocyclyl group is a 5-6 membered non-aromatic ringsystem having ring carbon atoms and 1-4 ring heteroatoms, wherein eachheteroatom is independently selected from nitrogen, oxygen, and sulfur(“5-6 membered heterocyclyl”). In some embodiments, the 5-6 memberedheterocyclyl has 1-3 ring heteroatoms selected from nitrogen, oxygen,and sulfur. In some embodiments, the 5-6 membered heterocyclyl has 1-2ring heteroatoms selected from nitrogen, oxygen, and sulfur. In someembodiments, the 5-6 membered heterocyclyl has one ring heteroatomselected from nitrogen, oxygen, and sulfur.

Exemplary 3-membered heterocyclyl groups containing one heteroatominclude, without limitation, azirdinyl, oxiranyl, thiorenyl. Exemplary4-membered heterocyclyl groups containing one heteroatom include,without limitation, azetidinyl, oxetanyl and thietanyl. Exemplary5-membered heterocyclyl groups containing one heteroatom include,without limitation, tetrahydrofuranyl, dihydrofuranyl,tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyland pyrrolyl-2,5-dione. Exemplary 5-membered heterocyclyl groupscontaining two heteroatoms include, without limitation, dioxolanyl,oxasulfuranyl, disulfuranyl, and oxazolidin-2-one. Exemplary 5-memberedheterocyclyl groups containing three heteroatoms include, withoutlimitation, triazolinyl, oxadiazolinyl, and thiadiazolinyl. Exemplary6-membered heterocyclyl groups containing one heteroatom include,without limitation, piperidinyl, tetrahydropyranyl, dihydropyridinyl,and thianyl. Exemplary 6-membered heterocyclyl groups containing twoheteroatoms include, without limitation, piperazinyl, morpholinyl,dithianyl, dioxanyl. Exemplary 6-membered heterocyclyl groups containingtwo heteroatoms include, without limitation, triazinanyl. Exemplary7-membered heterocyclyl groups containing one heteroatom include,without limitation, azepanyl, oxepanyl and thiepanyl. Exemplary8-membered heterocyclyl groups containing one heteroatom include,without limitation, azocanyl, oxecanyl and thiocanyl. Exemplary5-membered heterocyclyl groups fused to a C₆ aryl ring (also referred toherein as a 5,6-bicyclic heterocyclic ring) include, without limitation,indolinyl, isoindolinyl, dihydrobenzofuranyl, dihydrobenzothienyl,benzoxazolinonyl, and the like. Exemplary 6-membered heterocyclyl groupsfused to an aryl ring (also referred to herein as a 6,6-bicyclicheterocyclic ring) include, without limitation, tetrahydroquinolinyl,tetrahydroisoquinolinyl, and the like.

“Nitrogen-containing heterocyclyl” group means a 4- to 7-memberednon-aromatic cyclic group containing at least one nitrogen atom, forexample, but without limitation, morpholine, piperidine (e.g.2-piperidinyl, 3-piperidinyl and 4-piperidinyl), pyrrolidine (e.g.2-pyrrolidinyl and 3-pyrrolidinyl), azetidine, pyrrolidone, imidazoline,imidazolidinone, 2-pyrazoline, pyrazolidine, piperazine, and N-alkylpiperazines such as N-methyl piperazine. Particular examples includeazetidine, piperidone and piperazone.

“Hetero” when used to describe a compound or a group present on acompound means that one or more carbon atoms in the compound or grouphave been replaced by a nitrogen, oxygen, or sulfur heteroatom. Heteromay be applied to any of the hydrocarbyl groups described above such asalkyl, e.g., heteroalkyl, cycloalkyl, e.g., heterocyclyl, aryl, e.g.,heteroaryl, cycloalkenyl, e.g., cycloheteroalkenyl, and the like havingfrom 1 to 5, and particularly from 1 to 3 heteroatoms.

“Acyl” refers to a radical —C(O)R²⁰, where R²⁰ is hydrogen, substitutedor unsubstituted alkyl, substituted or unsubstituted alkenyl,substituted or unsubstituted alkynyl, substituted or unsubstitutedcarbocyclyl, substituted or unsubstituted heterocyclyl, substituted orunsubstituted aryl, or substituted or unsubstituted heteroaryl, asdefined herein. “Alkanoyl” is an acyl group wherein R²⁰ is a group otherthan hydrogen. Representative acyl groups include, but are not limitedto, formyl (—CHO), acetyl (—C(═O)CH₃), cyclohexylcarbonyl,cyclohexylmethylcarbonyl, benzoyl (—C(═O)Ph), benzylcarbonyl(—C(═O)CH₂Ph), C(O)—C₁-C₈ alkyl, —C(O)—(CH₂)_(t)(C₆-C₁₀ aryl),—C(O)—(CH₂)_(t)(5-10 membered heteroaryl), —C(O)—(CH₂)_(t)(C₃-C₁₀cycloalkyl), and —C(O)—(CH₂)_(t)(4-10 membered heterocyclyl), wherein tis an integer from 0 to 4. In certain embodiments, R²¹ is C₁-C₈ alkyl,substituted with halogen or hydroxy; or C₃-C₁₀ cycloalkyl, 4-10 memberedheterocyclyl, C₆-C₁₀ aryl, arylalkyl, 5-10 membered heteroaryl orheteroarylalkyl, each of which is substituted with unsubstitutedC₁-C₄alkyl, halogen, unsubstituted C₁-C₄ alkoxy, unsubstituted C₁-C₄haloalkyl, unsubstituted C₁-C₄ hydroxyalkyl, or unsubstituted C₁-C₄haloalkoxy or hydroxy.

“Alkoxy” refers to the group —OR²⁹ where R²⁹ is substituted orunsubstituted alkyl, substituted or unsubstituted alkenyl, substitutedor unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, substituted or unsubstitutedaryl, or substituted or unsubstituted heteroaryl. Particular alkoxygroups are methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy,tert-butoxy, sec-butoxy, n-pentoxy, n-hexoxy, and 1,2-dimethylbutoxy.Particular alkoxy groups are lower alkoxy, i.e. with between 1 and 6carbon atoms. Further particular alkoxy groups have between 1 and 4carbon atoms.

In certain embodiments, R²⁹ is a group that has 1 or more substituents,for instance from 1 to 5 substituents, and particularly from 1 to 3substituents, in particular 1 substituent, selected from the groupconsisting of amino, substituted amino, C₆-C₁₀ aryl, aryloxy, carboxyl,cyano, C₃-C₁₀ cycloalkyl, 4-10 membered heterocyclyl, halogen, 5-10membered heteroaryl, hydroxyl, nitro, thioalkoxy, thioaryloxy, thiol,alkyl-S(O)—, aryl-S(O)—, alkyl-S(O)₂— and aryl-S(O)₂-. Exemplary‘substituted alkoxy’ groups include, but are not limited to,—O—(CH₂)_(t)(C₆-C₁₀ aryl), —O—(CH₂)_(t)(5-10 membered heteroaryl),—O—(CH₂)_(t)(C₃-C₁₀ cycloalkyl), and —O—(CH₂)_(t)(4-10 memberedheterocyclyl), wherein t is an integer from 0 to 4 and any aryl,heteroaryl, cycloalkyl or heterocyclyl groups present, may themselves besubstituted by unsubstituted C₁-C₄ alkyl, halogen, unsubstituted C₁-C₄alkoxy, unsubstituted C₁-C₄ haloalkyl, unsubstituted C₁-C₄ hydroxyalkyl,or unsubstituted C₁-C₄ haloalkoxy or hydroxy. Particular exemplary‘substituted alkoxy’ groups are —OCF₃, —OCH₂CF₃, —OCH₂Ph,—OCH₂-cyclopropyl, —OCH₂CH₂OH, and —OCH₂CH₂NMe₂.

“Amino” refers to the radical —NH₂.

“Oxo group” refers to —C(═O)—.

“Substituted amino” refers to an amino group of the formula —N(R³⁸)₂wherein R³⁸ is hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted carbocyclyl, substituted or unsubstitutedheterocyclyl, substituted or unsubstituted aryl, substituted orunsubstituted heteroaryl, or an amino protecting group, wherein at leastone of R³⁸ is not a hydrogen. In certain embodiments, each R³⁸ isindependently selected from hydrogen, C₁-C₈ alkyl, C₃-C₈ alkenyl, C₃-C₈alkynyl, C₆-C₁₀ aryl, 5-10 membered heteroaryl, 4-10 memberedheterocyclyl, or C₃-C₁₀ cycloalkyl; or C₁-C₈ alkyl, substituted withhalogen or hydroxy; C₃-C₈ alkenyl, substituted with halogen or hydroxy;C₃-C₈ alkynyl, substituted with halogen or hydroxy, or —(CH₂)_(t)(C₆-C₁₀aryl), —(CH₂)_(t)(5-10 membered heteroaryl), —(CH₂)_(t)(C₃-C₁₀cycloalkyl), or —(CH₂)_(t)(4-10 membered heterocyclyl), wherein t is aninteger between 0 and 8, each of which is substituted by unsubstitutedC₁-C₄ alkyl, halogen, unsubstituted C₁-C₄ alkoxy, unsubstituted C₁-C₄haloalkyl, unsubstituted C₁-C₄ hydroxyalkyl, or unsubstituted C₁-C₄haloalkoxy or hydroxy; or both R³⁸ groups are joined to form an alkylenegroup.

Exemplary “substituted amino” groups include, but are not limited to,—NR³⁹—C₁-C₈ alkyl, —NR³⁹—(CH₂)_(t)(C₆-C₁₀ aryl), —NR³⁹—(CH₂)_(t)(5-10membered heteroaryl), —NR³⁹—(CH₂)_(t)(C₃-C₁₀ cycloalkyl), and—NR³⁹—(CH₂)_(t)(4-10 membered heterocyclyl), wherein t is an integerfrom 0 to 4, for instance 1 or 2, each R³⁹ independently represents H orC₁-C₈ alkyl; and any alkyl groups present, may themselves be substitutedby halogen, substituted or unsubstituted amino, or hydroxy; and anyaryl, heteroaryl, cycloalkyl, or heterocyclyl groups present, maythemselves be substituted by unsubstituted C₁-C₄ alkyl, halogen,unsubstituted C₁-C₄ alkoxy, unsubstituted C₁-C₄ haloalkyl, unsubstitutedC₁-C₄ hydroxyalkyl, or unsubstituted C₁-C₄ haloalkoxy or hydroxy. Forthe avoidance of doubt the term ‘substituted amino’ includes the groupsalkylamino, substituted alkylamino, alkylarylamino, substitutedalkylarylamino, arylamino, substituted arylamino, dialkylamino, andsubstituted dialkylamino as defined below. Substituted amino encompassesboth monosubstituted amino and disubstituted amino groups.

“Carboxy” refers to the radical —C(O)OH.

“Cyano” refers to the radical —CN.

“Halo” or “halogen” refers to fluoro (F), chloro (Cl), bromo (Br), andiodo (I). In certain embodiments, the halogen group is either fluoro orchloro.

“Haloalkyl” refers to an alkyl radical in which the alkyl group issubstituted with one or more halogens. Typical haloalkyl groups include,but are not limited to, trifluoromethyl, difluoromethyl, fluoromethyl,chloromethyl, dichloromethyl, dibromoethyl, tribromomethyl,tetrafluoroethyl, and the like.

“Hydroxy” refers to the radical —OH.

“Nitro” refers to the radical —NO₂.

“Thioketo” refers to the group ═S.

Alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, and heteroarylgroups, as defined herein, are optionally substituted (e.g.,“substituted” or “unsubstituted” alkyl, “substituted” or “unsubstituted”alkenyl, “substituted” or “unsubstituted” alkynyl, “substituted” or“unsubstituted” carbocyclyl, “substituted” or “unsubstituted”heterocyclyl, “substituted” or “unsubstituted” aryl or “substituted” or“unsubstituted” heteroaryl group). In general, the term “substituted”,whether preceded by the term “optionally” or not, means that at leastone hydrogen present on a group (e.g., a carbon or nitrogen atom) isreplaced with a permissible substituent, e.g., a substituent which uponsubstitution results in a stable compound, e.g., a compound which doesnot spontaneously undergo transformation such as by rearrangement,cyclization, elimination, or other reaction. Unless otherwise indicated,a “substituted” group has a substituent at one or more substitutablepositions of the group, and when more than one position in any givenstructure is substituted, the substituent is either the same ordifferent at each position. The term “substituted” is contemplated toinclude substitution with all permissible substituents of organiccompounds, any of the substituents described herein that results in theformation of a stable compound. The present invention contemplates anyand all such combinations in order to arrive at a stable compound. Forpurposes of this invention, heteroatoms such as nitrogen may havehydrogen substituents and/or any suitable substituent as describedherein which satisfy the valencies of the heteroatoms and results in theformation of a stable moiety.

Exemplary carbon atom substituents include, but are not limited to,halogen, —CN, —NO₂, —N₃, —SO₂H, —SO₃H, —OH, —OR^(aa), —ON(R^(bb))₂,—N(R^(bb))₂, —N(R^(bb))₃ ⁺X⁻, —N(OR^(cc))R^(bb), —SH, —SR—, —SSR^(cc),—C(═O)R^(aa), —CO₂H, —CHO, —C(OR^(cc))₂, —CO₂R^(aa), —OC(═O)R^(aa),—OCO₂R^(aa), —C(═O)N(R^(bb))₂, —OC(═O)N(R^(bb))₂, —NR^(bb)C(═O)R^(aa),—NR^(bb)CO₂R^(aa), —NR^(bb)C(═O)N(R^(bb))₂, —C(═NR^(bb))R^(aa),—C(═NR^(bb))OR^(aa), —OC(═NR^(bb))R^(aa), —OC(═NR^(bb))OR^(aa),—C(═NR^(bb))N(R^(bb))₂, —OC(═NR^(bb))N(R^(bb))₂,—NR^(bb)C(═NR^(bb))N(R^(bb))₂, —C(═O)NR^(bb)SO₂R^(aa),—NR^(bb)SO₂R^(aa), —SO₂N(R^(bb))₂, —SO₂R^(aa), —SO₂OR^(aa), —OSO₂R^(aa),—S(═O)R^(aa), —OS(═O)R^(aa), —Si(R^(aa))₃,—OSi(R^(aa))₃—C(═S)N(R^(bb))₂, —C(═O)SR^(aa), —C(═S)SR^(aa),—SC(═S)SR^(aa), —SC(═O)SR^(aa), —OC(═O)SR^(aa), —SC(═O)OR^(aa),—SC(═O)R^(aa), —P(═O)₂R^(aa), —OP(═O)₂R^(aa), —P(═O)(R^(aa))₂,—OP(═O)(R^(aa))₂, —OP(═O)(OR^(cc))₂, —P(═O)₂N(R^(bb))₂,—OP(═O)₂N(R^(bb))₂, —P(═O)(NR^(bb))₂, —OP(═O)(NR^(bb))₂,—NR^(bb)P(═O)(OR^(cc))₂, —NR^(bb)P(═O)(NR^(bb))₂, —P(R^(cc))₂,—P(R^(cc))₃, —OP(R^(cc))₂, —OP(R^(cc))₃, —B(R′)₂, —B(OR^(cc))₂,—BR^(aa)(OR^(cc)), C₁₋₁₀ alkyl, C₁₋₁₀ haloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀alkynyl, C₃₋₁₀ carbocyclyl, 3-14 membered heterocyclyl, C₆₋₁₄ aryl, and5-14 membered heteroaryl, wherein each alkyl, alkenyl, alkynyl,carbocyclyl, heterocyclyl, aryl, and heteroaryl is independentlysubstituted with 0, 1, 2, 3, 4, or 5 R^(dd) groups; or two geminalhydrogens on a carbon atom are replaced with the group ═O, ═S,═NN(R^(bb))₂, ═NNR^(bb)C(═O)R^(aa), ═NNR^(bb)C(═O)OR^(aa),═NNR^(bb)S(═O)₂R^(aa), ═NR^(bb), or ═NOR^(cc);

-   -   each instance of R^(aa) is, independently, selected from C₁₋₁₀        alkyl, C₁₋₁₀ haloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl, C₃₋₁₀        carbocyclyl, 3-14 membered heterocyclyl, C₆₋₁₄ aryl, and 5-14        membered heteroaryl, or two R^(aa) groups are joined to form a        3-14 membered heterocyclyl or 5-14 membered heteroaryl ring,        wherein each alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl,        aryl, and heteroaryl is independently substituted with 0, 1, 2,        3, 4, or 5 R^(dd) groups;    -   each instance of R^(bb) is, independently, selected from        hydrogen, —OH, —OR^(aa), —N(R^(cc))₂, —CN, —C(═O)R^(aa),        —C(═O)N(R^(cc))₂, —CO₂R^(aa), —SO₂R^(aa), —C(═NR^(cc))OR^(aa),        —C(═NR^(cc))N(R^(cc))₂, —SO₂N(R^(cc))₂, —SO₂R^(cc), —SO₂OR^(cc),        —SOR^(aa), —C(═S)N(R^(cc))₂, —C(═O)SR^(cc), —C(═S)SR^(cc),        —P(═O)₂R^(aa), —P(═O)(R^(aa))₂, —P(═O)₂N(R^(cc))₂,        —P(═O)(NR^(cc))₂, C₁₋₁₀ alkyl, C₁₋₁₀ haloalkyl, C₂₋₁₀ alkenyl,        C₂₋₁₀ alkynyl, C₃₋₁₀ carbocyclyl, 3-14 membered heterocyclyl,        C₆₋₁₄ aryl, and 5-14 membered heteroaryl, or two R^(bb) groups        are joined to form a 3-14 membered heterocyclyl or 5-14 membered        heteroaryl ring, wherein each alkyl, alkenyl, alkynyl,        carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently        substituted with 0, 1, 2, 3, 4, or 5 R^(dd) groups;    -   each instance of R^(cc) is, independently, selected from        hydrogen, C₁₋₁₀ alkyl, C₁₋₁₀ haloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀        alkynyl, C₃₋₁₀ carbocyclyl, 3-14 membered heterocyclyl, C₆₋₁₄        aryl, and 5-14 membered heteroaryl, or two R^(cc) groups are        joined to form a 3-14 membered heterocyclyl or 5-14 membered        heteroaryl ring, wherein each alkyl, alkenyl, alkynyl,        carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently        substituted with 0, 1, 2, 3, 4, or 5 R^(dd) groups;    -   each instance of R^(dd) is, independently, selected from        halogen, —CN, —NO₂, —N₃, —SO₂H, —SO₃H, —OH, —OR^(ee),        —ON(R^(ff))₂, —N(R^(ff))₂, —N(R^(ff))₃ ⁺X⁻, —N(OR^(ee))R^(ff),        —SH, —SR^(ee), —SSR^(ee), —C(═O)R^(ee), —CO₂H, —CO₂R^(ee),        —OC(═O)R^(ee), —OCO₂R^(ee), —C(═O)N(R^(ff))₂, —OC(═O)N(R^(ff))₂,        —NR^(ff)C(═O)R^(ee), —NR^(ff)CO₂R^(ee), —NR^(ff)C(═)N(R^(ff))₂,        —C(═NR^(ff))OR^(ee), —OC(═NR^(ff))R^(ee), —OC(═NR^(ff))OR^(ee),        —C(═NR^(ff))N(R^(ff))₂, —OC(═NR^(ff))N(R^(ff))₂,        —NR^(ff)C(═NR^(ff))N(R^(ff))₂, —NR^(ff)SO₂R^(ee),        —SO₂N(R^(ff))₂, —SO₂R^(ee), —SO₂OR^(ee), —OSO₂R^(ee),        —S(═O)R^(ee), —Si(R^(ee))₃, —OSi(R^(ee))₃, —C(═S)N(R^(ff))₂,        —C(═O)SR^(ee), —C(═S)SR^(ee), —SC(═S)SR^(ee), —P(═O)₂R^(ee),        —P(═O)(R^(ee))₂, —OP(═O)(R^(ee))₂, —OP(═O)(OR^(ee))₂, C₁₋₆        alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀        carbocyclyl, 3-10 membered heterocyclyl, C₆₋₁₀ aryl, 5-10        membered heteroaryl, wherein each alkyl, alkenyl, alkynyl,        carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently        substituted with 0, 1, 2, 3, 4, or 5 R^(gg) groups, or two        geminal R^(dd) substituents can be joined to form ═O or ═S;    -   each instance of R^(ee) is, independently, selected from C₁₋₆        alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₁₀        carbocyclyl, C₆₋₁₀ aryl, 3-10 membered heterocyclyl, and 3-10        membered heteroaryl, wherein each alkyl, alkenyl, alkynyl,        carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently        substituted with 0, 1, 2, 3, 4, or 5 R^(gg) groups;    -   each instance of R^(ff) is, independently, selected from        hydrogen, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆        alkynyl, C₃₋₁₀ carbocyclyl, 3-10 membered heterocyclyl, C₆₋₁₀        aryl and 5-10 membered heteroaryl, or two R^(ff) groups are        joined to form a 3-14 membered heterocyclyl or 5-14 membered        heteroaryl ring, wherein each alkyl, alkenyl, alkynyl,        carbocyclyl, heterocyclyl, aryl, and heteroaryl is independently        substituted with 0, 1, 2, 3, 4, or 5 R^(gg) groups; and    -   each instance of R^(gg) is, independently, halogen, —CN, —NO₂,        —N₃, —SO₂H, —SO₃H, —OH, —OC₁₋₆ alkyl, —ON(C₁₋₆ alkyl)₂, —N(C₁₋₆        alkyl)₂, —N(C₁₋₆ alkyl)₃ ⁺X⁻, —NH(C₁₋₆ alkyl)₂ ⁺X⁻, —NH₂(C₁₋₆        alkyl)⁺X⁻, —NH₃ ⁺X⁻, —N(OC₁₋₆ alkyl)(C₁₋₆ alkyl), —N(OH)(C₁₋₆        alkyl), —NH(OH), —SH, —SC₁₋₆ alkyl, —SS(C₁₋₆ alkyl), —C(═O)(C₁₋₆        alkyl), —CO₂H, —CO₂(C₁₋₆ alkyl), —OC(═O)(C₁₋₆ alkyl), —OCO₂(C₁₋₆        alkyl), —C(═O)NH₂, —C(═O)N(C₁₋₆ alkyl)₂, —OC(═O)NH(C₁₋₆ alkyl),        —NHC(═O)(C₁₋₆ alkyl), —N(C₁₋₆ alkyl)C(═O)(C₁₋₆ alkyl),        —NHCO₂(C₁₋₆ alkyl), —NHC(═O)N(C₁₋₆ alkyl)₂, —NHC(═O)NH(C₁₋₆        alkyl), —NHC(═O)NH₂, —C(═NH)O(C₁₋₆ alkyl), —OC(═NH)(C₁₋₆ alkyl),        —OC(═NH)OC₁₋₆ alkyl, —C(═NH)N(C₁₋₆ alkyl)₂, —C(═NH)NH(C₁₋₆        alkyl), —C(═NH)NH₂, —OC(═NH)N(C₁₋₆ alkyl)₂, —OC(NH)NH(C₁₋₆        alkyl), —OC(NH)NH₂, —NHC(NH)N(C₁₋₆ alkyl)₂, —NHC(═NH)NH₂,        —NHSO₂(C₁₋₆ alkyl), —SO₂N(C₁₋₆ alkyl)₂, —SO₂NH(C₁₋₆ alkyl),        —SO₂NH₂, —SO₂C₁₋₆ alkyl, —SO₂₀C₁₋₆ alkyl, —OSO₂C₁₋₆ alkyl,        —SOC₁₋₆ alkyl, —Si(C₁₋₆ alkyl)₃, —OSi(C₁₋₆ alkyl)₃, —C(═S)N(C₁₋₆        alkyl)₂, C(═S)NH(C₁₋₆ alkyl), C(═S)NH₂, —C(═O)S(C₁₋₆ alkyl),        —C(═S)SC₁₋₆ alkyl, —SC(═S)SC₁₋₆ alkyl, —P(═O)₂(C₁₋₆ alkyl),        —P(═O)(C₁₋₆ alkyl)₂, —OP(═O)(C₁₋₆ alkyl)₂, —OP(═O)(OC₁₋₆        alkyl)₂, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl,        C₃₋₁₀ carbocyclyl, C₆₋₁₀ aryl, 3-10 membered heterocyclyl, 5-10        membered heteroaryl; or two geminal R^(gg) substituents can be        joined to form O or ═S; wherein X is a counterion.

In some embodiments, carbon atom substituents include halogen, —CN, —OH,—OR^(aa), —N(R^(bb))₂, —CO₂H, —CO₂R^(aa),—OC(═O)R^(aa),—C(═O)N(R^(bb))₂, —SO₂R^(aa), C₁₋₆ alkyl, C₁₋₆ haloalkyl,C₃₋₁₀ carbocyclyl, 5-6 membered heterocyclyl, phenyl, and 5-6 memberedheteroaryl, wherein each instance of R^(aa) is, independently, selectedfrom hydrogen, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl,C₃₋₁₀ carbocyclyl, 5-6 membered heterocyclyl, phenyl, and 5-6 memberedheteroaryl; and

-   -   each instance of R^(bb) is, independently, selected from        hydrogen, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₂₋₁₀ alkenyl, C₂₋₁₀        alkynyl, C₃₋₁₀ carbocyclyl, 5-6 membered heterocyclyl, phenyl,        and 5-6 membered heteroaryl.

A “counterion” or “anionic counterion” is a negatively charged groupassociated with a cationic quaternary amino group in order to maintainelectronic neutrality. Exemplary counterions include halide ions (e.g.,F⁻, Cl⁻, Br⁻, I⁻), NO₃ ⁻, ClO₄ ⁻, OH⁻, H₂PO₄ ⁻, HSO₄ ⁻, sulfonate ions(e.g., methansulfonate, trifluoromethanesulfonate, p-toluenesulfonate,benzenesulfonate, 10-camphor sulfonate, naphthalene-2-sulfonate,naphthalene-1-sulfonic acid-5-sulfonate, ethan-1-sulfonicacid-2-sulfonate, and the like), and carboxylate ions (e.g., acetate,ethanoate, propanoate, benzoate, glycerate, lactate, tartrate,glycolate, and the like).

These and other exemplary substituents are described in more detail inthe Detailed Description, and Claims. The invention is not intended tobe limited in any manner by the above exemplary listing of substituents.

Other Definitions

As used herein, the term “modulation” refers to the inhibition orpotentiation of GABA_(A) receptor function. A “modulator” (e.g., amodulator compound) may be, for example, an agonist, partial agonist,antagonist, or partial antagonist of the GABA_(A) receptor.

“Pharmaceutically acceptable” means approved or approvable by aregulatory agency of the Federal or a state government or thecorresponding agency in countries other than the United States, or thatis listed in the U.S. Pharmacopoeia or other generally recognizedpharmacopoeia for use in animals, and more particularly, in humans.

“Pharmaceutically acceptable salt” refers to a salt of a compound of theinvention that is pharmaceutically acceptable and that possesses thedesired pharmacological activity of the parent compound. In particular,such salts are non-toxic may be inorganic or organic acid addition saltsand base addition salts. Specifically, such salts include: (1) acidaddition salts, formed with inorganic acids such as hydrochloric acid,hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and thelike; or formed with organic acids such as acetic acid, propionic acid,hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid,lactic acid, malonic acid, succinic acid, malic acid, maleic acid,fumaric acid, tartaric acid, citric acid, benzoic acid,3-(4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid,methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid,2-hydroxyethanesulfonic acid, benzenesulfonic acid,4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid,4-toluenesulfonic acid, camphorsulfonic acid,4-methylbicyclo[2.2.2]-oct-2-ene-1-carboxylic acid, glucoheptonic acid,3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid,lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoicacid, salicylic acid, stearic acid, muconic acid, and the like; or (2)salts formed when an acidic proton present in the parent compound eitheris replaced by a metal ion, e.g., an alkali metal ion, an alkaline earthion, or an aluminum ion; or coordinates with an organic base such asethanolamine, diethanolamine, triethanolamine, N-methylglucamine and thelike. Salts further include, by way of example only, sodium, potassium,calcium, magnesium, ammonium, tetraalkylammonium, and the like; and whenthe compound contains a basic functionality, salts of non-toxic organicor inorganic acids, such as hydrochloride, hydrobromide, tartrate,mesylate, acetate, maleate, oxalate and the like. The term“pharmaceutically acceptable cation” refers to an acceptable cationiccounter-ion of an acidic functional group. Such cations are exemplifiedby sodium, potassium, calcium, magnesium, ammonium, tetraalkylammoniumcations, and the like. See, e.g., Berge, et al., J. Pharm. Sci. (1977)66(1): 1-79.

The term “prodrug” is intended to encompass therapeutically inactivecompounds that, under physiological conditions, are converted into thetherapeutically active agents of the present invention. One method formaking a prodrug is to design selected moieties that are hydrolyzed orcleaved at a targeted in vivo site of action under physiologicalconditions to reveal the desired molecule which then produces itstherapeutic effect. In certain embodiments, the prodrug is converted byan enzymatic activity of the subject.

In an alternate embodiment, the present invention provides prodrugs ofcompound of Formula (I), wherein the prodrug includes a cleavable moietyon the C₃ hydroxy as depicted in Formula (I).

“Tautomers” refer to compounds that are interchangeable forms of aparticular compound structure, and that vary in the displacement ofhydrogen atoms and electrons. Thus, two structures may be in equilibriumthrough the movement of 7 electrons and an atom (usually H). Forexample, enols and ketones are tautomers because they are rapidlyinterconverted by treatment with either acid or base. Another example oftautomerism is the aci- and nitro-forms of phenylnitromethane, that arelikewise formed by treatment with acid or base. Tautomeric forms may berelevant to the attainment of the optimal chemical reactivity andbiological activity of a compound of interest.

A “subject” to which administration is contemplated includes, but is notlimited to, humans (i.e., a male or female of any age group, e.g., apediatric subject (e.g., infant, child, adolescent) or adult subject(e.g., young adult, middle-aged adult or senior adult)) and/or anon-human animal, e.g., a mammal such as primates (e.g., cynomolgusmonkeys, rhesus monkeys), cattle, pigs, horses, sheep, goats, rodents,cats, and/or dogs. In certain embodiments, the subject is a human. Incertain embodiments, the subject is a non-human animal.

In certain embodiments, the substituent present on an oxygen atom is anoxygen protecting group (also referred to as a hydroxyl protectinggroup). Oxygen protecting groups include, but are not limited to,—R^(aa), —N(R^(bb))₂, —C(═O)SR^(aa), —C(═O)R^(aa), —CO₂R^(aa),—C(═O)N(R^(bb))₂, —C(═NR^(bb))R^(aa), —C(═NR^(bb))OR^(aa),—C(═NR^(bb))N(R^(bb))₂, —S(═O)R^(aa), —SO₂R^(aa), —Si(R^(aa))₃,—P(R^(cc))₂, —P(R^(cc))₃, —P(═O)₂R^(aa), —P(═O)(R^(aa))₂,—P(═O)(OR^(cc))₂, —P(═O)₂N(R^(bb))₂, and —P(═O)(NR^(bb))₂, whereinR^(aa), R^(bb), and R^(cc) are as defined herein. Oxygen protectinggroups are well known in the art and include those described in detailin Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M.Wuts, 3^(rd) edition, John Wiley & Sons, 1999, incorporated herein byreference.

Exemplary oxygen protecting groups include, but are not limited to,methyl, methoxylmethyl (MOM), 2-methoxyethoxymethyl (MEM), benzyl (Bn),triisopropylsilyl (TIPS), t-butyldimethylsilyl (TBDMS),t-butylmethoxyphenylsilyl (TBMPS), methanesulfonate (mesylate), andtosylate (Ts).

In certain embodiments, the substituent present on an sulfur atom is ansulfur protecting group (also referred to as a thiol protecting group).Sulfur protecting groups include, but are not limited to, —R^(aa),—N(R^(bb))₂, —C(═O)SR^(aa), —C(═O)R^(aa), —CO₂R^(aa), —C(═O)N(R^(bb))₂,—C(═NR^(bb))R^(aa), —C(═NR^(bb))OR^(aa), —C(═NR^(bb))N(R^(bb))₂,—S(═O)R^(aa), —SO₂R^(aa), —Si(R^(aa))₃, —P(R^(cc))₂, —P(R^(aa))₃,—P(═O)₂R^(aa), —P(═O)(R^(aa))₂, —P(═O)(OR^(cc))₂, —P(═O)₂N(R^(bb))₂, and—P(═O)(NR^(bb))₂, wherein R^(aa), R^(bb), and R^(cc) are as definedherein. Sulfur protecting groups are well known in the art and includethose described in detail in Protecting Groups in Organic Synthesis, T.W. Greene and P. G. M. Wuts, 3^(rd) edition, John Wiley & Sons, 1999,incorporated herein by reference.

In certain embodiments, the substituent present on a nitrogen atom is anamino protecting group (also referred to herein as a nitrogen protectinggroup). Amino protecting groups include, but are not limited to, —OH,—OR^(aa), —N(R^(cc))₂, —C(═O)R^(aa), —C(═O)OR^(aa), —C(═O)N(R^(cc))₂,—S(═O)₂R^(aa), —C(═NR^(cc))R^(aa), —C(═NR^(cc))OR^(aa),—C(═NR^(cc))N(R^(cc))₂, —SO₂N(R^(cc))₂, —SO₂R^(cc), —SO₂OR^(cc),—SOR^(aa), —C(═S)N(R^(cc))₂, —C(═O)SR^(cc), —C(═S)SR^(cc), C₁₋₁₀ alkyl,C₂₋₁₀ alkenyl, C₂₋₁₀ alkynyl, C₃₋₁₀ carbocyclyl, 3-14-memberedheterocyclyl, C₆₋₁₄ aryl, and 5-14-membered heteroaryl groups, whereineach alkyl, alkenyl, alkynyl, carbocyclyl, heterocyclyl, aryl, andheteroaryl is independently substituted with 0, 1, 2, 3, 4, or 5 R^(dd)groups, and wherein R^(aa), R^(bb), R^(cc) and R^(dd) are as definedherein. Amino protecting groups are well known in the art and includethose described in detail in Protecting Groups in Organic Synthesis, T.W. Greene and P. G. M. Wuts, 3^(rd) edition, John Wiley & Sons, 1999,incorporated herein by reference.

Exemplary amino protecting groups include, but are not limited to amidegroups (e.g., —C(═O)R^(aa)), which include, but are not limited to,formamide and acetamide; carbamate groups (e.g., —C(═O)OR^(aa)), whichinclude, but are not limited to, 9-fluorenylmethyl carbamate (Fmoc),t-butyl carbamate (BOC), and benzyl carbamate (Cbz); sulfonamide groups(e.g., —S(═O)₂R^(aa)), which include, but are not limited to,p-toluenesulfonamide (Ts), methanesulfonamide (Ms), andN-[2-(trimethylsilyl)ethoxy]methylamine (SEM).

Disease, disorder, and condition are used interchangeably herein.

As used herein, and unless otherwise specified, the terms “treat,”“treating” and “treatment” contemplate an action that occurs while asubject is suffering from the specified disease, disorder or condition,which reduces the severity of the disease, disorder or condition, orretards or slows the progression of the disease, disorder or condition.In an alternate embodiment, the present invention contemplatesadministration of the compounds of the present invention as aprophylactic before a subject begins to suffer from the specifieddisease, disorder or condition.

In general, the “effective amount” of a compound refers to an amountsufficient to elicit the desired biological response, e.g., to treat aCNS-related disorder, is sufficient to induce anesthesia or sedation. Aswill be appreciated by those of ordinary skill in this art, theeffective amount of a compound of the invention may vary depending onsuch factors as the desired biological endpoint, the pharmacokinetics ofthe compound, the disease being treated, the mode of administration, andthe age, weight, health, and condition of the subject. An effectiveamount encompasses therapeutic and prophylactic treatment.

As used herein, and unless otherwise specified, a “therapeuticallyeffective amount” of a compound is an amount sufficient to provide atherapeutic benefit in the treatment of a disease, disorder orcondition, or to delay or minimize one or more symptoms associated withthe disease, disorder or condition. A therapeutically effective amountof a compound means an amount of therapeutic agent, alone or incombination with other therapies, which provides a therapeutic benefitin the treatment of the disease, disorder or condition. The term“therapeutically effective amount” can encompass an amount that improvesoverall therapy, reduces or avoids symptoms or causes of disease orcondition, or enhances the therapeutic efficacy of another therapeuticagent.

As used herein, and unless otherwise specified, a “prophylacticallyeffective amount” of a compound is an amount sufficient to prevent adisease, disorder or condition, or one or more symptoms associated withthe disease, disorder or condition, or prevent its recurrence. Aprophylactically effective amount of a compound means an amount of atherapeutic agent, alone or in combination with other agents, whichprovides a prophylactic benefit in the prevention of the disease,disorder or condition. The term “prophylactically effective amount” canencompass an amount that improves overall prophylaxis or enhances theprophylactic efficacy of another prophylactic agent.

Alternative Embodiments

In an alternative embodiment, compounds described herein may alsocomprise one or more isotopic substitutions. For example, hydrogen maybe ²H (D or deuterium) or ³H (T or tritium); carbon may be, for example,¹³C or ¹⁴C; oxygen may be, for example, ¹⁸O; nitrogen may be, forexample, ¹⁵N, and the like. In other embodiments, a particular isotope(e.g., ³H, ¹³C, ¹⁴C, ¹⁸O, or ¹⁵N) can represent at least 1%, at least5%, at least 10%, at least 15%, at least 20%, at least 25%, at least30%, at least 35%, at least 40%, at least 45%, at least 50%, at least60%, at least 65%, at least 70%, at least 75%, at least 80%, at least85%, at least 90%, at least 95%, at least 99%, or at least 99.9% of thetotal isotopic abundance of an element that occupies a specific site ofthe compound.

Compounds

It should be appreciated that formulas described herein may referenceparticular carbon atoms, such as C17, C3, C19, etc. These references arebased on the position of carbon atoms according to steroid nomenclatureknown and used in the industry, as shown below:

For example, C17 refers to the carbon at position 17 and C3 refers tothe carbon at position 3.

In an aspect, provided herein is a compound of Formula (I)

or a pharmaceutically acceptable salt thereof;wherein:

-   -   represents a single or double bond, provided if a double bond is        present, then one of R^(6a) or R^(6b) is absent and R⁵ is        absent;    -   L is selected from the group consisting of:

wherein A indicates the point of attachment at C17;

-   -   X is selected from the group consisting of        —C(O)N(R^(55a))(R^(55b)), —N(R^(55a))(R^(55b))        N(R^(55b))C(O)(R^(55a)), and R^(55c);    -   R^(Y) is each independently hydrogen, cyano, haloalkyl, or        unsubstituted alkyl;    -   R^(55c) is carbon-bound substituted or unsubstituted heteroaryl        or substituted or unsubstituted aryl;    -   R^(55a) and R^(55b) is each independently hydrogen, substituted        or unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, —OR^(A1), —N(R^(A1))₂—SR^(A1),        —C(═O)R^(A1), —C(═O)OR^(A1), —C(═O)SR^(A1), —C(═O)N(R^(A1))₂,        —OC(═O)R^(A1), —OC(═O)OR^(A1), —OC(═O)N(R^(A1))₂,        —OC(═O)SR^(A1), —OS(═O)₂R^(A1), —OS(═O)₂OR^(A1),        —OS(═O)₂N(R^(A1))₂, —N(R^(A1))C(═O)R^(A1),        —N(R^(A1))C(═NR^(A1))R^(A1), —N(R^(A1))C(═O)OR^(A1),        —N(R^(A1))C(═O)N(R^(A1))₂, —N(R^(A1))C(═NR^(A1)) N(R^(A1))₂,        —N(R^(A1))S(═O)₂R^(A1), —N(R^(A1))S(═O)₂OR^(A1),        —N(R^(A1))S(═O)₂N(R^(A1))₂, —SC(═O)R^(A1), —SC(═O)OR^(A1),        —SC(═O)SR^(A1), —SC(═O)N(R^(A1))₂, —S(═O)₂R^(A1),        —S(═O)₂OR^(A1), or —S(═O)₂N(R^(A1))₂, wherein each instance of        R^(A1) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, or substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, a sulfur protecting group when attached to sulfur, or        two R^(A1) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring;    -   or R^(55a) and R^(55b) may join together with the intervening        atoms to form a substituted or unsubstituted heterocyclyl or a        substituted or unsubstituted heteroaryl;    -   each of R^(1a), R^(1b), R^(2a), R^(2b), R^(4a), R^(4b), R^(7a),        R^(7b), R^(11a), R^(11b), R^(12a), and R^(12b) is independently        hydrogen, halogen, cyano, —NO₂, substituted or unsubstituted        alkyl, substituted or unsubstituted alkenyl, substituted or        unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,        substituted or unsubstituted heterocyclyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        —OR^(A1), —N(R^(A1))₂, —SR^(A1), —C(═O)R^(A1), —C(═O)OR^(A1),        —C(═O)SR^(A1), —C(═O)N(R^(A1))₂, —OC(═O)R^(A1), —OC(═O)OR^(A1),        —OC(═O)N(R^(A1))₂, —OC(═O)SR^(A1), —OS(═O)₂R^(A1),        —OS(═O)₂OR^(A1), —OS(═O)₂N(R^(A1))₂, —N(R^(A1))C(═O)R^(A1),        —N(R^(A1))C(═NR^(A1))R^(A1), —N(R^(A1))C(═O)OR^(A1),        —N(R^(A1))C(═O)N(R^(A1))₂, —N(R^(A1))C(═NR^(A1)) N(R^(A1))₂,        —N(R^(A1))S(═O)₂R^(A1), —N(R^(A1))S(═O)₂OR^(A1),        —N(R^(A1))S(═O)₂N(R^(A1))₂, —SC(═O)R^(A1), —SC(═O)OR^(A1),        —SC(═O)SR^(A1), —SC(═O)N(R^(A1))₂, —S(═O)₂R^(A1),        —S(═O)₂OR^(A1), or —S(═O)₂N(R^(A1))₂, wherein each instance of        R^(A1) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, or substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, or a sulfur protecting group when attached to sulfur;        or R^(11a) and R^(11b) are joined to form an oxo (═O) group; or        R^(12a) and R^(12b) are joined to form an oxo (═O) group; or        R^(4a) and R^(4b) are joined to form an oxo (═O) group; or        R^(7a) and R^(7b) are joined to form an oxo (═O) group; or        R^(2a) and R^(2b) are joined to form an oxo (═O) group; or        R^(1a) and R^(1b) are joined to form an oxo (═O) group;    -   R^(3a) is substituted or unsubstituted alkyl, substituted or        unsubstituted alkenyl, substituted or unsubstituted alkynyl,        substituted or unsubstituted carbocyclyl, substituted or        unsubstituted heterocyclyl, substituted or unsubstituted aryl,        or substituted or unsubstituted heteroaryl;    -   R⁵ is hydrogen or substituted or unsubstituted alkyl;    -   each of R^(6a) and R^(6b) is hydrogen, halogen, cyano, —NO₂,        —OH, substituted or unsubstituted alkyl, substituted or        unsubstituted alkenyl, or substituted or unsubstituted alkynyl;        or R^(6a) and R^(6b) are joined to form an oxo (═O) group;    -   R^(D) is independently hydrogen, halogen, —CN, —NO₂, oxo,        substituted or unsubstituted alkyl, substituted or unsubstituted        alkenyl, substituted or unsubstituted alkynyl, substituted or        unsubstituted carbocyclyl, substituted or unsubstituted        heterocyclyl, substituted or unsubstituted aryl, substituted or        unsubstituted heteroaryl, —OR^(C3), —N(R^(C3))₂, —SR^(C3),        —C(═O)R^(C3), —C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂,        —OC(═O)R^(C3), —OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂,        —OC(═O)SR^(C3), —OS(═O)₂R^(C3), —OS(═O)₂OR^(C3),        —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),        —N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),        —N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,        —N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),        —N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),        —SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3),        —S(═O)₂OR^(C3), or —S(═O)₂N(R^(C3))₂, wherein each instance of        R^(C3) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        substituted or unsubstituted carbocyclyl, or substituted or        unsubstituted heterocyclyl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, or a sulfur protecting group when attached to sulfur;    -   R¹⁸ is substituted or unsubstituted alkyl;    -   R¹⁹ is hydrogen, substituted or unsubstituted alkyl, substituted        or unsubstituted alkenyl, or substituted or unsubstituted        alkynyl; and    -   q is an integer from 0 to 5;    -   provided that the compound is not:

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-I):

or a pharmaceutically acceptable salt thereof, wherein

-   -   each of R^(15a), R^(15b), R^(16a), and R^(16b) is independently        hydrogen, halogen, —CN, —NO₂, substituted or unsubstituted        alkyl, substituted or unsubstituted alkenyl, substituted or        unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,        substituted or unsubstituted heterocyclyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        —OR^(C3), —N(R^(C3))₂, —SR^(C3), —C(═O)R^(C3), —C(═O)OR^(C3),        —C(═O)SR^(C3), —C(═O)N(R^(C3))₂, —OC(═O)R^(C3), —OC(═O)OR^(C3),        —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3), —OS(═O)₂R^(C3),        —OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),        —N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),        —N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,        —N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),        —N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),        —SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3),        —S(═O)₂OR^(C3), or —S(═O)₂N(R^(C3))₂, wherein each instance of        R^(C3) is independently selected from hydrogen, substituted or        unsubstituted alkyl, substituted or unsubstituted alkenyl,        substituted or unsubstituted alkynyl, substituted or        unsubstituted aryl, substituted or unsubstituted heteroaryl,        substituted or unsubstituted carbocyclyl, or substituted or        unsubstituted heterocyclyl, an oxygen protecting group when        attached to oxygen, a nitrogen protecting group when attached to        nitrogen, a sulfur protecting group when attached to sulfur; or        R^(15a) and R^(15b) are joined to form an oxo (═O) group; or        R^(16a) and R^(16b) are joined to form an oxo (═O) group.

In some embodiments, the compound is a compound of Formula (I-a):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Ia):

or a pharmaceutically acceptable salt thereof.

Groups R^(55a) and R^(55b)

In some embodiments, R^(55c) is hydrogen or methyl and R^(55b) issubstituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, substituted orunsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, or substituted or unsubstitutedheteroaryl.

In some embodiments, R^(55a) and R^(55b) is each independently hydrogen,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, substituted orunsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, or substituted or unsubstitutedheteroaryl.

In some embodiments, R^(55a) and R^(55b) is each independently hydrogen,substituted or unsubstituted carbocyclyl, substituted or unsubstitutedheterocyclyl, substituted or unsubstituted aryl, or substituted orunsubstituted heteroaryl.

In some embodiments, R^(55a) and R^(55b) is each independentlysubstituted carbocyclyl, substituted heterocyclyl, substituted aryl, orsubstituted heteroaryl.

In some embodiments, at least R^(55a) or R^(55b) is other than hydrogen.

In some embodiments, R^(55a) and R^(55b) is each independently hydrogen,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl,

wherein:

-   -   each instance of R^(a) is independently hydrogen, halogen, —NO₂,        —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), —S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), —S(═O₂N(R⁴)₂,or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 11.

In some embodiments, R^(55a) and R^(55b) is each independently hydrogen,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl,

wherein:

-   -   each instance of R^(a) is independently hydrogen, halogen, —NO₂,        —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 3.

In some embodiments, R^(55a) and R^(55b) is each independently hydrogen,substituted or unsubstituted alkyl,

wherein:

-   -   each instance of R^(a) is independently hydrogen, halogen, —NO₂,        —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 5.

In some embodiments, R^(55a) and R^(55b) is independently hydrogen,substituted or unsubstituted alkyl,

wherein:

-   -   each instance of R^(a) is independently hydrogen, halogen, —CN,        —OR^(D4), —N(R^(D4))₂, -substituted or unsubstituted C₁₋₆ alkyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen or substituted        or unsubstituted C₁₋₆ alkyl; and    -   p is an integer selected from 0 to 2.

In some embodiments, when R^(a) is substituted 3- to 6-memberedheterocyclyl or substituted 5- to 10-membered heteroaryl, the 3- to6-membered heterocyclyl or 5- to 10-membered heteroaryl are substitutedwith one or more of C₁₋₆alkyl, cyano, or oxo. For example, in certainembodiments, R^(a) is a 5- to 6-membered heterocyclyl substituted withone or more of C₁₋₆alkyl, cyano, or oxo or a 5- to 6-membered heteroarylsubstituted with one or more of C₁₋₆alkyl, cyano, or oxo.

In some embodiments, R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl.

In some embodiments, R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl selected from the groupconsisting of:

wherein:

-   -   each instance of R^(a) is independently hydrogen, oxo, halogen,        —NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 10.

In some embodiments, R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl selected from the groupconsisting of:

wherein:

-   -   each instance of R^(a) is independently hydrogen, oxo, halogen,        —NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 10.

In some embodiments, R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl selected from the groupconsisting of:

wherein:

-   -   each instance of R^(a) is independently hydrogen, oxo, halogen,        —CN, —OR^(D4), —N(R^(D4))₂, or substituted or unsubstituted C₁₋₆        alkyl;    -   each instance of R^(D4) is independently hydrogen or substituted        or unsubstituted C₁₋₆ alkyl; and    -   p is an integer selected from 0 to 2.        Groups R^(1a) and R^(1b)

In some embodiments, R^(1a) and R^(1b) are each independently hydrogen,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, substituted orunsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl.

In some embodiments, R^(1a) and R^(1b) are each independently hydrogen,substituted or unsubstituted carbocyclyl, substituted or unsubstitutedheterocyclyl, substituted or unsubstituted aryl, substituted orunsubstituted heteroaryl.

In some embodiments, R^(1a) and R^(1b) are each independentlysubstituted carbocyclyl, substituted heterocyclyl, substituted aryl, orsubstituted heteroaryl, wherein each is further substituted withsubstituted carbocyclyl, substituted heterocyclyl, substituted aryl, orsubstituted heteroaryl.

In some embodiments, R^(1a) and R^(1b) are each independently selectedfrom the group consisting of:

wherein:

-   -   each instance of R^(a) is independently hydrogen, halogen, —NO₂,        —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 11.

In some embodiments, R^(1a) and R^(1b) are each independently selectedfrom the group consisting of:

wherein:

-   -   each instance of R^(a) is independently hydrogen, halogen, —NO₂,        —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),        —C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4),        —N(R^(D4))C(═O)R^(D4), —OC(═O)N(R^(D4))₂,        —N(R^(D4))C(═O)OR^(D4), —S(═O)₂R^(D4), —S(═O)₂OR^(D4),        —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),        substituted or unsubstituted C₁₋₆ alkyl, substituted or        unsubstituted C₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆        alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl,        substituted or unsubstituted 3- to 6-membered heterocyclyl,        substituted or unsubstituted C₅₋₁₀ aryl, substituted or        unsubstituted 5- to 10-membered heteroaryl;    -   each instance of R^(D4) is independently hydrogen, substituted        or unsubstituted C₁₋₆ alkyl, substituted or unsubstituted C₂₋₆        alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted        or unsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted        3- to 6-membered heterocyclyl, substituted or unsubstituted        C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-membered        heteroaryl, an oxygen protecting group when attached to oxygen,        a nitrogen protecting group when attached to nitrogen, or two        R^(D4) groups are taken with the intervening atoms to form a        substituted or unsubstituted heterocyclic ring; and    -   p is an integer selected from 0 to 11.

In some embodiments, R^(1a) and R^(1b) are both hydrogen.

Groups R^(2a) and R^(2b)

In some embodiments, R^(2a) and R^(2b) are each independently hydrogen,halogen, —CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.

In some embodiments, R^(2a) and R^(2b) are each independently hydrogen,halogen, —CN, —NO₂, —OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or—N(R^(F6))C(═O)R^(F6); wherein each instance of R^(F6) is independentlyhydrogen, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,or two R^(F6) groups are taken with the intervening atoms to form asubstituted or unsubstituted heterocyclic ring.

In some embodiments, R^(2a) and R^(2b) are independently hydrogen, —OH,or substituted or unsubstituted C₁₋₆ alkyl.

In some embodiments, each of R^(2a) and R^(2b) are independentlyhydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆alkoxyhalo.

In some embodiments, R^(2a) and R^(2b) are independently —CH₃, —CH₂CH₃,—OH, —OCH₃, or —CH(CH₃)₂.

In some embodiments, R^(2a) and R^(2b) are both hydrogen.

In some embodiments, R^(2a) and R^(2b) are joined to form an oxo (═O)group.

Group R^(3a)

In some embodiments, R^(3a) is substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, or substituted or unsubstitutedalkynyl.

In some embodiments, R^(3a) is hydrogen, substituted or unsubstitutedalkyl, or substituted or unsubstituted carbocyclyl.

In some embodiments, R^(3a) is substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, substituted or unsubstitutedaryl, or substituted or unsubstituted heteroaryl.

In some embodiments, R^(3a) is substituted or unsubstituted carbocyclyl.In some embodiments, R^(3a) is cyclopropyl.

In some embodiments, R^(3a) is substituted or unsubstituted alkyl orsubstituted or unsubstituted carbocyclyl.

In some embodiments, R^(3a) is substituted or unsubstituted C₁₋₆alkyl.

In some embodiments, R³ is substituted alkyl. In some embodiments,R^(3a) is unsubstituted alkyl.

In some embodiments, R^(3a) is methyl, ethyl, n-propyl, iso-propyl,n-butyl, iso-butyl. In some embodiments, R^(3a) is methyl. In someembodiments, R^(3a) is ethyl. In some embodiments, R^(3a) is propyl. Insome embodiments, R^(3a) is n-butyl.

In some embodiments, R^(3a) is substituted C₁₋₆ alkyl.

In some embodiments, R^(3a) is —CH₂C₃H₅.

In some embodiments, R^(3a) is C₁₋₆ alkoxy.

In some embodiments, R^(3a) is —CH₂OCH₃, —CH₂CH₂OCH₃, or —CH₂CH₂CH₂OCH₃.

In some embodiments, R^(3a) is —CH₂OCH₂CH₃, —CH₂CH₂OCH₂CH₃, or—CH₂CH₂CH₂OCH₂CH₃.

In some embodiments, R^(3a) is —CH₂OCH₂CH₂CH₃, —CH₂CH₂OCH₂CH₂CH₃, or—CH₂CH₂CH₂OCH₂CH₂CH₃.

Groups R^(4a) and R^(4b)

In some embodiments, R^(4a) and R^(4b) is each independently hydrogen,halogen, —CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.

In some embodiments, R^(4a) and R^(4b) is each independently hydrogen,halogen, —CN, —NO₂, —OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or—N(R^(F6))C(═O)R^(F6); wherein each instance of R^(F6) is independentlyhydrogen, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,or two R^(F6) groups are taken with the intervening atoms to form asubstituted or unsubstituted heterocyclic ring.

In some embodiments, R^(4a) and R^(4b) are independently hydrogen, —OH,or substituted or unsubstituted C₁₋₆ alkyl.

In some embodiments, each of R^(4a) and R^(4b) are independentlyhydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆alkoxyhalo.

In some embodiments, R^(4a) and R^(4b) are independently —CH₃, —CH₂CH₃,—OH, —OCH₃, or —CH(CH₃)₂.

In some embodiments, R^(4a) and R^(4b) are both hydrogen.

In some embodiments, R^(4a) and R^(4b) are joined to form an oxo (═O)group.

Group R⁵

In some embodiments, R⁵ is hydrogen or methyl in the cis position,relative to R¹⁹ or in the trans position, relative to R¹⁹.

In some embodiments, R⁵ is hydrogen in the cis position, relative to R¹⁹or in the trans position, relative to R¹⁹. In some embodiments, R⁵ ishydrogen in the cis position, relative to R¹⁹. In some embodiments, R⁵is hydrogen in the trans position, relative to R¹⁹.

In some embodiments, R⁵ is methyl in the cis position, relative to R¹⁹or in the trans position, relative to R¹⁹. In some embodiments, R⁵ ismethyl in the cis position, relative to R¹⁹. In some embodiments, R⁵ ismethyl in the trans position, relative to R¹⁹.

Group R^(6a) and R^(6b)

In some embodiments, R^(6a) and R^(6b) is independently hydrogen,halogen, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, or substituted or unsubstituted alkynyl.

In some embodiments, R^(6a) and R^(6b) is independently hydrogen orsubstituted alkyl.

In some embodiments, R^(6a) and R^(6b) is independently hydrogen orunsubstituted alkyl.

In some embodiments, R^(6a) is halogen or alkyl and R^(6b) is hydrogen.

In some embodiments, R^(6a) and R^(6b) are both halogen.

In some embodiments, R^(6a) and R^(6b) are both unsubstituted alkyl.

In some embodiments, R^(6a) is hydrogen and R^(6b) is absent.

Groups R^(7a) and R^(7b)

In some embodiments, R^(7a) and R^(7b) is each independently hydrogen,halogen, —CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.

In some embodiments, R^(7a) and R^(7b) is each independently hydrogen,halogen, —CN, —NO₂, —OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or—N(R^(F6))C(═O)R^(F6); wherein each instance of R^(F6) is independentlyhydrogen, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,or two R^(F6) groups are taken with the intervening atoms to form asubstituted or unsubstituted heterocyclic ring.

In some embodiments, R^(7a) and R^(7b) are independently hydrogen, —OH,or substituted or unsubstituted C₁₋₆ alkyl.

In some embodiments, each of R^(7a) and R^(7b) are independentlyhydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆alkoxyhalo.

In some embodiments, R^(7a) and R^(7b) are independently —CH₃, —CH₂CH₃,—OH, —OCH₃, or —CH(CH₃)₂.

In some embodiments, R^(7a) and R^(7b) are both hydrogen.

In some embodiments, R^(7a) and R^(7b) are joined to form an oxo (═O)group.

Groups R^(11a) and R^(11b)

In some embodiments, R^(11a) and R^(b) is each independently hydrogen,halogen, —CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.

In some embodiments, R^(11a) and R^(11b) is each independently hydrogen,halogen, —CN, —NO₂, —OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or—N(R^(F6))C(═O)R^(F6); wherein each instance of R^(F6) is independentlyhydrogen, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,or two R^(F6) groups are taken with the intervening atoms to form asubstituted or unsubstituted heterocyclic ring.

In some embodiments, R^(11a) and R^(11b) are independently hydrogen,—OH, or substituted or unsubstituted C₁₋₆ alkyl.

In some embodiments, each of R^(11a) and R^(11b) are independentlyhydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆alkoxyhalo.

In some embodiments, R^(11a) and R^(11b) are independently hydrogen,—OH, or R^(11a) and R¹ b are joined to form an oxo (═O) group.

In some embodiments, R^(11a) and R^(11b) are independently —CH₃,—CH₂CH₃, —OH, —OCH₃, or —CH(CH₃)₂.

In some embodiments, R^(11a) and R^(11b) are both hydrogen.

In some embodiments, R^(11a) and R^(11b) are joined to form an oxo (═O)group.

Groups R^(12a) and R^(12b)

In some embodiments, R^(12a) and R^(12b) is each independently hydrogen,halogen, —CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.

In some embodiments, R^(12a) and R^(12b) is each independently hydrogen,halogen, —CN, —NO₂, —OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or—N(R^(F6))C(═O)R^(F6); wherein each instance of R^(F6) is independentlyhydrogen, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,or two R^(F6) groups are taken with the intervening atoms to form asubstituted or unsubstituted heterocyclic ring.

In some embodiments, R^(12a) and R^(12b) are independently hydrogen,—OH, or substituted or unsubstituted C₁₋₆ alkyl.

In some embodiments, each of R^(12a) and R^(12b) are independentlyhydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆alkoxyhalo.

In some embodiments, R^(12a) and R^(12b) are independently —CH₃,—CH₂CH₃, —OH, —OCH₃, or —CH(CH₃)₂.

In some embodiments, R^(12a) and R^(12b) are both hydrogen.

In some embodiments, R^(12a) and R^(12b) are joined to form an oxo (═O)group.

Group R¹⁹

In some embodiments, R¹⁹ is hydrogen, substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, or substituted orunsubstituted alkynyl.

In some embodiments, R¹⁹ is hydrogen or substituted or unsubstitutedalkyl.

In some embodiments, R¹⁹ is substituted alkyl.

In some embodiments, R¹⁹ is substituted C₂-C₆ alkyl.

In some embodiments, R¹⁹ is —CH₂OCH₃. In some embodiments, R¹⁹ is—CH₂OCH₂CH₃.

In some embodiments, R¹⁹ is hydrogen or unsubstituted alkyl.

In some embodiments, R¹⁹ is unsubstituted alkyl.

In some embodiments, R¹⁹ is unsubstituted C₁-C₆ alkyl.

In some embodiments, R¹⁹ is methyl. In some embodiments, R¹⁹ is ethyl.

In some embodiments, R¹⁹ is hydrogen or substituted or unsubstitutedC₁-C₆ alkyl.

In some embodiments, R¹⁹ is hydrogen, methyl, ethyl, or methoxymethyl.

Group R¹⁸

In some embodiments, R¹⁸ is substituted alkyl. In some embodiments, R¹⁸is substituted C₁₋₆alkyl.

In some embodiments, R¹⁸ is unsubstituted alkyl. In some embodiments,R¹⁸ is unsubstituted C₁-C₆ alkyl. In some embodiments, R¹⁸ is methyl. Insome embodiments, R¹⁸ is ethyl.

Group R^(D)

In some embodiments, R^(D) is independently hydrogen, halogen, —CN,—NO₂, oxo, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, —OR^(C3), —N(R^(C3))₂, —SR^(C3), —C(═O)R^(C3),—C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂, —OC(═O)R^(C3),—OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3), —OS(═O)₂R^(C3),—OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),—N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),—N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,—N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),—N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),—SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3), —S(═O)₂OR^(C3), or—S(═O)₂N(R^(C3))₂, wherein each instance of R^(C3) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl.

In some embodiments, R^(D) is each independently hydrogen, halogen, —CN,—NO₂, oxo, hydroxy, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, orsubstituted or unsubstituted carbocyclyl.

In some embodiments, R^(D) is each independently hydrogen, oxo,substituted or unsubstituted alkyl, hydroxy, or substituted orunsubstituted carbocyclyl.

In some embodiments, R^(D) is independently hydrogen, oxo, methyl,ethyl, hydroxy, or cyclopropyl.

Groups R^(15a) and R^(15b)

In some embodiments, each of R^(15a) and R^(15b) is each independentlyhydrogen, halogen, —CN, —NO₂, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl,substituted or unsubstituted heteroaryl, —OR^(C3), —N(R^(C3))₂,—SR^(C3), —C(═O)R^(C3), —C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂,—OC(═O)R^(C3), —OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3),—OS(═O)₂R^(C3), —OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂,—N(R^(C3))C(═O)R^(C3), —N(R^(C3))C(═NR^(C3))R^(C3),—N(R^(C3))C(═O)OR^(C3), —N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3))N(R^(C3))₂, —N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),—N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),—SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3), —S(═O)₂OR^(C3), or—S(═O)₂N(R^(C3))₂, wherein each instance of R^(C3) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl, an oxygen protecting group when attached tooxygen, a nitrogen protecting group when attached to nitrogen, a sulfurprotecting group when attached to sulfur, or two R^(C3) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring; or R^(15a) and R^(15b) are joined to form an oxo (═O)group.

In some embodiments, each of R^(15a) and R^(15b) is each independentlyhydrogen, halogen, —CN, substituted or unsubstituted alkyl, orsubstituted or unsubstituted carbocyclyl.

In some embodiments, R^(15a) and R^(15b) are both hydrogen.

In some embodiments, R^(15a) and R^(15b) are joined to form an oxo (═O)group.

In some embodiments, R^(15a) and R^(15b) is each independently hydrogen,substituted or unsubstituted alkyl, or substituted or unsubstitutedcarbocyclyl. In some embodiments, R^(15a) and R^(15b) is eachindependently hydrogen, unsubstituted alkyl, or unsubstitutedcarbocyclyl. In some embodiments, R^(15a) and R^(15b) is eachindependently hydrogen, methyl, or cyclopropyl.

Groups R^(16a) and R^(16b)

In some embodiments, each of R^(16a) and R^(16b) is each independentlyhydrogen, halogen, —CN, —NO₂, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl,substituted or unsubstituted heteroaryl, —OR^(C3), —N(R^(C3))₂,—SR^(C3), —C(═O)R^(C3), —C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂,—OC(═O)R^(C3), —OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3),—OS(═O)₂R^(C3), —OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂,—N(R^(C3))C(═O)R^(C3), —N(R^(C3))C(═NR^(C3))R^(C3),—N(R^(C3))C(═O)OR^(C3), —N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3))N(R^(C3))₂, —N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),—N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),—SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3), —S(═O)₂OR^(C3), or—S(═O)₂N(R^(C3))₂, wherein each instance of R^(C3) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl, an oxygen protecting group when attached tooxygen, a nitrogen protecting group when attached to nitrogen, a sulfurprotecting group when attached to sulfur, or two R^(C3) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring; or R^(16a) and R^(16b) are joined to form an oxo (═O)group.

In some embodiments, each of R^(16a) and R^(16b) is each independentlyhydrogen, halogen, —CN, substituted or unsubstituted alkyl, orsubstituted or unsubstituted carbocyclyl.

In some embodiments, R^(16a) and R^(16b) are both hydrogen.

In some embodiments, R^(16a) and R^(16b) are joined to form an oxo (═O)group.

L

In some embodiments, L is

In some embodiments, R^(Y) is hydrogen, unsubstituted C₁₋₆alkyl,unsubstituted C₁₋₆haloalkyl, or cyano. In certain embodiments, R ishydrogen, methyl, ethyl, —CF₃, or cyano.

In some embodiments, L is

In some embodiments, L is

In some embodiments, L is

In some embodiments, L is

In some embodiments, L is

In some embodiments, L is

X

In some embodiments, X is —NC(O)(R^(55a))

In some embodiments, X is —N(R^(55a))(R^(55b)).

In some embodiments, X is —C(O)N(R^(55a))(R^(55b)).

In some embodiments, X is R^(55c).

Group R^(55c)

In some embodiments, R^(55c) is substituted or unsubstituted phenyl orcarbon-bound substituted or unsubstituted heteroaryl containing at leastone nitrogen in the heteroaryl ring.

In some embodiments, R^(55c) is substituted or unsubstituted phenyl orcarbon-bound substituted or unsubstituted heteroaryl selected from thegroup consisting of pyridyl, isothiazolyl, thiazolyl, pyrimidyl,pyrazinyl, and oxazolyl.

In some embodiments, R^(55c) is selected from the group consisting of:

-   -   wherein:    -   each instance of R^(a) is independently hydrogen, halogen, —CN,        —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4), or        substituted or unsubstituted C₁₋₆ alkyl;    -   each instance of R^(D4) is independently hydrogen or substituted        or unsubstituted C₁₋₆ alkyl; and    -   p is an integer selected from 0 to 2.

In some embodiments, the compound is a compound of Formula (I-b), (I-c),(I-d), (I-e), (I-l), (I-m), (I-n), or (I-p):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-f), (I-g),or (I-h):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-i), (I-j),or (I-k):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-o):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-qq),(I-q), (I-s), (I-t), or (I-u):

or a pharmaceutically acceptable salt thereof, wherein

-   -   Q, Q′, and Q″ are each independently CR^(w) or N;    -   R^(w) is hydrogen, cyano, —NH₂, or substituted or unsubstituted        alkyl; and    -   at least one of Q, Q′, and Q″ is CR^(w).

In some embodiments, the compound is a compound of Formula (I-r):

or a pharmaceutically acceptable salt thereof, wherein

-   -   k is an integer 1 or 2;    -   R^(z) is substituted or unsubstituted alkyl or substituted or        unsubstituted aryl; or two R^(z)s on adjacent carbons combine        with the intervening atoms to form a substituted or        unsubstituted aryl; and    -   j is an integer 0-6.

In some embodiments, the compound is a compound of Formula (I-v), (I-w),or (I-x):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Ib),(I-Ic), (I-Id), (I-Ie), (I-Il), (I-Im), (I-In), (I-Ip1), or (I-Ip2):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-If),(1-Ig), or (I-Ih):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Ii),(I-Ij), or (I-Ik):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Io1) or(I-Io2):

or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound is a compound of Formula (I-Iqq),(I-Iq1), (I-Iq2), (I-It1), (I-It2), (I-Iu1), or (I-Iu2):

or a pharmaceutically acceptable salt thereof, wherein

-   -   Q, Q′, and Q″ are each independently CR^(w) or N;    -   R^(w) is hydrogen, cyano, —NH₂, or substituted or unsubstituted        alkyl; and    -   at least one of Q, Q′, and Q″ is CR^(w).

In some embodiments, the compound is a compound of Formula (I-Irr),(I-Ir1) or (I-Ir2):

or a pharmaceutically acceptable salt thereof, wherein

-   -   k is an integer 1 or 2;    -   R^(z) is substituted or unsubstituted alkyl or substituted or        unsubstituted aryl; or two R^(z)s on adjacent carbons combine        with the intervening atoms to form a substituted or        unsubstituted aryl; and    -   j an integer 0-6.

In some embodiments, the compound is a compound of Formula (I-Ir3) or(I-Ir4):

or a pharmaceutically acceptable salt thereof, wherein

-   -   k is an integer 1 or 2;    -   R^(z′) is substituted or unsubstituted alkyl or cyano; and    -   j′ an integer 0-4.

In some embodiments, the compound is a compound of Formula (I-Iw1),(I-Iw2), (I-Ix1), or (I-Ix2):

or a pharmaceutically acceptable salt thereof.

It should be appreciated that the stereochemistry at C17 could bedepicted in any of the following but equivalent ways:

Compounds of the present invention as described herein, act, in certainembodiments, as GABA_(A) receptor modulators. In certain embodiments,the compounds described herein can act as positive allosteric modulatorsof the GABA receptor e.g., of the GABA_(A) receptor.

In one embodiment, the compounds described herein (e.g., a compound ofFormula I or Table 1) exhibit higher selectivity for modulation of theα4β3δ configuration of GABA_(A) receptor relative to the α1β2γ2configuration of GABA_(A) receptor.

As modulators of the excitability of the central nervous system (CNS),as mediated by their ability to modulate GABA_(A) receptor, suchcompounds are expected to have CNS-activity.

In some embodiments, the compound is selected from the group consistingof the compounds identified in Table 1 below:

TABLE 1 Example STRUCTURE 1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

87

88

89

90

91

92

93

94

95

96

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

150

151

152

153

154

155

156

157

158

159

160

161

162

163

164

201

202

203

204

205

206

207

208

209

210

211

212

213

214

250

251

252

253

254

255

256

257

258

259

260

261

262

263

264

265

266

267

268

269

270

271

272

273

274

275

276

278

279

280

281

282

283

285

286

287

288

289

290

293

294

295

296

297

298

299

300

301

302

303

304

305

306

307

308

309

310

311

312

313

314

315

316

317

318

319

320

321

322

323

324

325

326

327

328

329

330

331

332

333

334

335

336

337

338

339

340

341

342

343

344

345

346

347

348

349

350

351

352

353

354

355

356

357

358

359

360

361

362

363

364

365

366

367

368

369

400

401

402

403

404

405

406

407

410

411

412

413

414

415

416

417

418

419

420

421

422

423

424

425

426

427

428

429

430

431

432

433

434

435

436

437

438

439

440

441

442

443

444

445

446

447

448

449

450

451

452

453

454

455

456

457

458

459

460

487

488

489

490

491

492

493

494

495

496

497

498

499

500

501

502

503

504

505

506

Exemplary compounds of the invention may be synthesized from thefollowing known starting materials using methods known to one skilled inthe art or certain references, In one aspect, provided herein is apharmaceutically acceptable salt of a compound described herein (e.g., acompound of Formula (I)).

Pharmaceutical Compositions

In one aspect, provided herein is a pharmaceutical compositioncomprising a compound described herein (e.g., a compound of Formula (I))or a pharmaceutically acceptable salt thereof, and a pharmaceuticallyacceptable excipient. In certain embodiments, the compound of thepresent invention is provided in an effective amount in thepharmaceutical composition. In certain embodiments, the compound of thepresent invention is provided in a therapeutically effective amount. Incertain embodiments, the compound of the present invention is providedin a prophylactically effective amount.

In certain embodiments, the pharmaceutical composition comprises aneffective amount of the active ingredient. In certain embodiments, thepharmaceutical composition comprises a therapeutically effective amountof the active ingredient. In certain embodiments, the pharmaceuticalcomposition comprises a prophylactically effective amount of the activeingredient.

The pharmaceutical compositions provided herein can be administered by avariety of routes including, but not limited to, oral (enteral)administration, parenteral (by injection) administration, rectaladministration, transdermal administration, intradermal administration,intrathecal administration, subcutaneous (SC) administration,intravenous (IV) administration, intramuscular (IM) administration, andintranasal administration.

Generally, the compounds provided herein are administered in aneffective amount. The amount of the compound actually administered willtypically be determined by a physician, in the light of the relevantcircumstances, including the condition to be treated, the chosen routeof administration, the actual compound administered, the age, weight,and response of the individual patient, the severity of the patient'ssymptoms, and the like.

When used to prevent the onset of a CNS-disorder, the compounds providedherein will be administered to a subject at risk for developing thecondition, typically on the advice and under the supervision of aphysician, at the dosage levels described above. Subjects at risk fordeveloping a particular condition generally include those that have afamily history of the condition, or those who have been identified bygenetic testing or screening to be particularly susceptible todeveloping the condition.

The pharmaceutical compositions provided herein can also be administeredchronically (“chronic administration”). Chronic administration refers toadministration of a compound or pharmaceutical composition thereof overan extended period of time, e.g., for example, over 3 months, 6 months,1 year, 2 years, 3 years, 5 years, etc, or may be continuedindefinitely, for example, for the rest of the subject's life. Incertain embodiments, the chronic administration is intended to provide aconstant level of the compound in the blood, e.g., within thetherapeutic window over the extended period of time.

The pharmaceutical compositions of the present invention may be furtherdelivered using a variety of dosing methods. For example, in certainembodiments, the pharmaceutical composition may be given as a bolus,e.g., in order to raise the concentration of the compound in the bloodto an effective level. The placement of the bolus dose depends on thesystemic levels of the active ingredient desired throughout the body,e.g., an intramuscular or subcutaneous bolus dose allows a slow releaseof the active ingredient, while a bolus delivered directly to the veins(e.g., through an IV drip) allows a much faster delivery which quicklyraises the concentration of the active ingredient in the blood to aneffective level. In other embodiments, the pharmaceutical compositionmay be administered as a continuous infusion, e.g., by IV drip, toprovide maintenance of a steady-state concentration of the activeingredient in the subject's body. Furthermore, in still yet otherembodiments, the pharmaceutical composition may be administered as firstas a bolus dose, followed by continuous infusion.

The compositions for oral administration can take the form of bulkliquid solutions or suspensions, or bulk powders. More commonly,however, the compositions are presented in unit dosage forms tofacilitate accurate dosing. The term “unit dosage forms” refers tophysically discrete units suitable as unitary dosages for human subjectsand other mammals, each unit containing a predetermined quantity ofactive material calculated to produce the desired therapeutic effect, inassociation with a suitable pharmaceutical excipient. Typical unitdosage forms include prefilled, premeasured ampules or syringes of theliquid compositions or pills, tablets, capsules or the like in the caseof solid compositions. In such compositions, the compound is usually aminor component (from about 0.1 to about 50% by weight or preferablyfrom about 1 to about 40% by weight) with the remainder being variousvehicles or excipients and processing aids helpful for forming thedesired dosing form.

With oral dosing, one to five and especially two to four and typicallythree oral doses per day are representative regimens. Using these dosingpatterns, each dose provides from about 0.01 to about 20 mg/kg of thecompound provided herein, with preferred doses each providing from about0.1 to about 10 mg/kg, and especially about 1 to about 5 mg/kg.

Transdermal doses are generally selected to provide similar or lowerblood levels than are achieved using injection doses, generally in anamount ranging from about 0.01 to about 20% by weight, preferably fromabout 0.1 to about 20% by weight, preferably from about 0.1 to about 10%by weight, and more preferably from about 0.5 to about 15% by weight.

Injection dose levels range from about 0.1 mg/kg/hour to at least 20mg/kg/hour, all for from about 1 to about 120 hours and especially 24 to96 hours. A preloading bolus of from about 0.1 mg/kg to about 10 mg/kgor more may also be administered to achieve adequate steady statelevels. The maximum total dose is not expected to exceed about 5 g/dayfor a 40 to 80 kg human patient.

Liquid forms suitable for oral administration may include a suitableaqueous or nonaqueous vehicle with buffers, suspending and dispensingagents, colorants, flavors and the like. Solid forms may include, forexample, any of the following ingredients, or compounds of a similarnature: a binder such as microcrystalline cellulose, gum tragacanth orgelatin; an excipient such as starch or lactose, a disintegrating agentsuch as alginic acid, Primogel, or corn starch; a lubricant such asmagnesium stearate; a glidant such as colloidal silicon dioxide; asweetening agent such as sucrose or saccharin; or a flavoring agent suchas peppermint, methyl salicylate, or orange flavoring.

Injectable compositions are typically based upon injectable sterilesaline or phosphate-buffered saline or other injectable excipients knownin the art. As before, the active compound in such compositions istypically a minor component, often being from about 0.05 to 10% byweight with the remainder being the injectable excipient and the like.

Transdermal compositions are typically formulated as a topical ointmentor cream containing the active ingredient(s). When formulated as anointment, the active ingredients will typically be combined with eithera paraffinic or a water-miscible ointment base. Alternatively, theactive ingredients may be formulated in a cream with, for example anoil-in-water cream base. Such transdermal formulations are well-known inthe art and generally include additional ingredients to enhance thedermal penetration of stability of the active ingredients orformulation. All such known transdermal formulations and ingredients areincluded within the scope provided herein.

The compounds provided herein can also be administered by a transdermaldevice. Accordingly, transdermal administration can be accomplishedusing a patch either of the reservoir or porous membrane type, or of asolid matrix variety.

The above-described components for orally administrable, injectable ortopically administrable compositions are merely representative. Othermaterials as well as processing techniques and the like are set forth inPart 8 of Remington's Pharmaceutical Sciences, 17th edition, 1985, MackPublishing Company, Easton, Pa., which is incorporated herein byreference.

The compounds of the present invention can also be administered insustained release forms or from sustained release drug delivery systems.A description of representative sustained release materials can be foundin Remington's Pharmaceutical Sciences.

The present invention also relates to the pharmaceutically acceptableacid addition salt of a compound of the present invention. The acidwhich may be used to prepare the pharmaceutically acceptable salt isthat which forms a non-toxic acid addition salt, i.e., a salt containingpharmacologically acceptable anions such as the hydrochloride,hydroiodide, hydrobromide, nitrate, sulfate, bisulfate, phosphate,acetate, lactate, citrate, tartrate, succinate, maleate, fumarate,benzoate, para-toluenesulfonate, and the like.

In another aspect, the invention provides a pharmaceutical compositioncomprising a compound of the present invention and a pharmaceuticallyacceptable excipient, e.g., a composition suitable for injection, suchas for intravenous (IV) administration.

Pharmaceutically acceptable excipients include any and all diluents orother liquid vehicles, dispersion or suspension aids, surface activeagents, isotonic agents, preservatives, lubricants and the like, assuited to the particular dosage form desired, e.g., injection. Generalconsiderations in the formulation and/or manufacture of pharmaceuticalcompositions agents can be found, for example, in Remington'sPharmaceutical Sciences, Sixteenth Edition, E. W. Martin (MackPublishing Co., Easton, Pa., 1980), and Remington: The Science andPractice of Pharmacy, 21^(st) Edition (Lippincott Williams & Wilkins,2005).

For example, injectable preparations, such as sterile injectable aqueoussuspensions, can be formulated according to the known art using suitabledispersing or wetting agents and suspending agents. Exemplary excipientsthat can be employed include, but are not limited to, water, sterilesaline or phosphate-buffered saline, or Ringer's solution.

In certain embodiments, the pharmaceutical composition further comprisesa cyclodextrin derivative. The most common cyclodextrins are α-, β- andγ-cyclodextrins consisting of 6, 7 and 8 α-1,4-linked glucose units,respectively, optionally comprising one or more substituents on thelinked sugar moieties, which include, but are not limited to,substituted or unsubstituted methylated, hydroxyalkylated, acylated, andsulfoalkylether substitution. In certain embodiments, the cyclodextrinis a sulfoalkyl ether β-cyclodextrin, e.g., for example, sulfobutylether β-cyclodextrin, also known as CAPTISOL®. See, e.g., U.S. Pat. No.5,376,645. In certain embodiments, the composition compriseshexapropyl-β-cyclodextrin. In a more particular embodiment, thecomposition comprises hexapropyl-β-cyclodextrin (10-50% in water).

The injectable composition can be sterilized, for example, by filtrationthrough a bacterial-retaining filter, or by incorporating sterilizingagents in the form of sterile solid compositions which can be dissolvedor dispersed in sterile water or other sterile injectable medium priorto use.

Generally, the compounds provided herein are administered in aneffective amount. The amount of the compound actually administered willtypically be determined by a physician, in the light of the relevantcircumstances, including the condition to be treated, the chosen routeof administration, the actual compound administered, the age, weight,response of the individual patient, the severity of the patient'ssymptoms, and the like.

The compositions are presented in unit dosage forms to facilitateaccurate dosing. The term “unit dosage forms” refers to physicallydiscrete units suitable as unitary dosages for human subjects and othermammals, each unit containing a predetermined quantity of activematerial calculated to produce the desired therapeutic effect, inassociation with a suitable pharmaceutical excipient. Typical unitdosage forms include pre-filled, pre-measured ampules or syringes of theliquid compositions. In such compositions, the compound is usually aminor component (from about 0.1% to about 50% by weight or preferablyfrom about 1% to about 40% by weight) with the remainder being variousvehicles or carriers and processing aids helpful for forming the desireddosing form.

The compounds provided herein can be administered as the sole activeagent, or they can be administered in combination with other activeagents. In one aspect, the present invention provides a combination of acompound of the present invention and another pharmacologically activeagent. Administration in combination can proceed by any techniqueapparent to those of skill in the art including, for example, separate,sequential, concurrent, and alternating administration.

Although the descriptions of pharmaceutical compositions provided hereinare principally directed to pharmaceutical compositions which aresuitable for administration to humans, it will be understood by theskilled artisan that such compositions are generally suitable foradministration to animals of all sorts. Modification of pharmaceuticalcompositions suitable for administration to humans in order to renderthe compositions suitable for administration to various animals is wellunderstood, and the ordinarily skilled veterinary pharmacologist candesign and/or perform such modification with ordinary experimentation.General considerations in the formulation and/or manufacture ofpharmaceutical compositions can be found, for example, in Remington: TheScience and Practice of Pharmacy 21^(st) ed., Lippincott Williams &Wilkins, 2005.

In one aspect, provided is a kit comprising a composition (e.g., a solidcomposition) comprising a compound of Formula (I).

Methods of Use and Treatment

In an aspect, compounds described herein, e.g., compounds of Formula(I), are envisioned to be useful as therapeutic agents for treating aCNS-related disorder (e.g., sleep disorder, a mood disorder such asdepression, a schizophrenia spectrum disorder, a convulsive disorder,epileptogenesis, a disorder of memory and/or cognition, a movementdisorder, a personality disorder, autism spectrum disorder, pain,traumatic brain injury, a vascular disease, a substance abuse disorderand/or withdrawal syndrome, or tinnitus) in a subject in need (e.g., asubject with Rett syndrome, Fragile X syndrome, or Angelman syndrome).Exemplary CNS conditions related to GABA-modulation include, but are notlimited to, sleep disorders [e.g., insomnia], mood disorders [e.g.,depression (e.g., major depressive disorder (MDD)), dysthymic disorder(e.g., mild depression), bipolar disorder (e.g., I and/or II), anxietydisorders (e.g., generalized anxiety disorder (GAD), social anxietydisorder), stress, post-traumatic stress disorder (PTSD), compulsivedisorders (e.g., obsessive compulsive disorder (OCD))], schizophreniaspectrum disorders [e.g., schizophrenia, schizoaffective disorder],convulsive disorders [e.g., epilepsy (e.g., status epilepticus (SE)),seizures], disorders of memory and/or cognition [e.g., attentiondisorders (e.g., attention deficit hyperactivity disorder (ADHD)),dementia (e.g., Alzheimer's type dementia, Lewis body type dementia,vascular type dementia], movement disorders [e.g., Huntington's disease,Parkinson's disease], personality disorders [e.g., anti-socialpersonality disorder, obsessive compulsive personality disorder], autismspectrum disorders (ASD) [e.g., autism, monogenetic causes of autismsuch as synaptophathy's, e.g., Rett syndrome, Fragile X syndrome,Angelman syndrome], pain [e.g., neuropathic pain, injury related painsyndromes, acute pain, chronic pain], traumatic brain injury (TBI),vascular diseases [e.g., stroke, ischemia, vascular malformations],substance abuse disorders and/or withdrawal syndromes [e.g., addition toopiates, cocaine, and/or alcohol], and tinnitus.

In certain embodiments, CNS-related disorder is a sleep disorder, a mooddisorder, a schizophrenia spectrum disorder, a convulsive disorder, adisorder of memory and/or cognition, a movement disorder, a personalitydisorder, autism spectrum disorder, pain, traumatic brain injury, avascular disease, a substance abuse disorder and/or withdrawal syndrome,tinnitus, or status epilepticus. In certain embodiments, the CNS-relateddisorder is depression. In certain embodiments, the CNS-related disorderis postpartum depression. In certain embodiments, the CNS-relateddisorder is major depressive disorder. In certain embodiments, the majordepressive disorder is moderate major depressive disorder. In certainembodiments, the major depressive disorder is severe major depressivedisorder.

In an aspect, provided is a method of alleviating or preventing seizureactivity in a subject, comprising administering to the subject in needof such treatment an effective amount of a compound of the presentinvention. In some embodiments, the method alleviates or preventsepileptogenesis.

In yet another aspect, provided is a combination of a compound of thepresent invention and another pharmacologically active agent. Thecompounds provided herein can be administered as the sole active agentor they can be administered in combination with other agents.Administration in combination can proceed by any technique apparent tothose of skill in the art including, for example, separate, sequential,concurrent and alternating administration.

In another aspect, provided is a method of treating or preventing brainexcitability in a subject susceptible to or afflicted with a conditionassociated with brain excitability, comprising administering to thesubject an effective amount of a compound of the present invention tothe subject.

In yet another aspect, provided is a method of treating or preventingstress or anxiety in a subject, comprising administering to the subjectin need of such treatment an effective amount of a compound of thepresent invention, or a composition thereof.

In yet another aspect, provided is a method of alleviating or preventinginsomnia in a subject, comprising administering to the subject in needof such treatment an effective amount of a compound of the presentinvention, or a composition thereof.

In yet another aspect, provided is a method of inducing sleep andmaintaining substantially the level of REM sleep that is found in normalsleep, wherein substantial rebound insomnia is not induced, comprisingadministering an effective amount of a compound of the presentinvention.

In yet another aspect, provided is a method of alleviating or preventingpremenstrual syndrome (PMS) or postnatal depression (PND) in a subject,comprising administering to the subject in need of such treatment aneffective amount of a compound of the present invention.

In yet another aspect, provided is a method of treating or preventingmood disorders in a subject, comprising administering to the subject inneed of such treatment an effective amount of a compound of the presentinvention. In certain embodiments the mood disorder is depression.

In yet another aspect, provided is a method of cognition enhancement ortreating memory disorder by administering to the subject atherapeutically effective amount of a compound of the present invention.In certain embodiments, the disorder is Alzheimer's disease. In certainembodiments, the disorder is Rett syndrome.

In yet another aspect, provided is a method of treating attentiondisorders by administering to the subject a therapeutically effectiveamount of a compound of the present invention. In certain embodiments,the attention disorder is ADHD.

In certain embodiments, the compound is administered to the subjectchronically. In certain embodiments, the compound is administered to thesubject orally, subcutaneously, intramuscularly, or intravenously.

Neuroendocrine Disorders and Dysfunction

Provided herein are methods that can be used for treating neuroendocrinedisorders and dysfunction. As used herein, “neuroendocrine disorder” or“neuroendocrine dysfunction” refers to a variety of conditions caused byimbalances in the body's hormone production directly related to thebrain. Neuroendocrine disorders involve interactions between the nervoussystem and the endocrine system. Because the hypothalamus and thepituitary gland are two areas of the brain that regulate the productionof hormones, damage to the hypothalamus or pituitary gland, e.g., bytraumatic brain injury, may impact the production of hormones and otherneuroendocrine functions of the brain. In some embodiments, theneuroendocrine disorder or dysfunction is associated with a women'shealth disorder or condition (e.g., a women's health disorder orcondition described herein). In some embodiments, the neuroendocrinedisorder or dysfunction is associated with a women's health disorder orcondition is polycystic ovary syndrome.

Symptoms of neuroendocrine disorder include, but are not limited to,behavioral, emotional, and sleep-related symptoms, symptoms related toreproductive function, and somatic symptoms; including but not limitedto fatigue, poor memory, anxiety, depression, weight gain or loss,emotional lability, lack of concentration, attention difficulties, lossof lipido, infertility, amenorrhea, loss of muscle mass, increased bellybody fat, low blood pressure, reduced heart rate, hair loss, anemia,constipation, cold intolerance, and dry skin.

Neurodegenerative Diseases and Disorders

The methods described herein can be used for treating neurodegenerativediseases and disorders. The term “neurodegenerative disease” includesdiseases and disorders that are associated with the progressive loss ofstructure or function of neurons, or death of neurons. Neurodegenerativediseases and disorders include, but are not limited to, Alzheimer'sdisease (including the associated symptoms of mild, moderate, or severecognitive impairment); amyotrophic lateral sclerosis (ALS); anoxic andischemic injuries; ataxia and convulsion (including for the treatmentand prevention and prevention of seizures that are caused byschizoaffective disorder or by drugs used to treat schizophrenia);benign forgetfulness; brain edema; cerebellar ataxia including McLeodneuroacanthocytosis syndrome (MLS); closed head injury; coma; contusiveinjuries (e.g., spinal cord injury and head injury); dementias includingmulti-infarct dementia and senile dementia; disturbances ofconsciousness; Down syndrome; drug-induced or medication-inducedParkinsonism (such as neuroleptic-induced acute akathisia, acutedystonia, Parkinsonism, or tardive dyskinesia, neuroleptic malignantsyndrome, or medication-induced postural tremor); epilepsy; fragile Xsyndrome; Gilles de la Tourette's syndrome; head trauma; hearingimpairment and loss; Huntington's disease; Lennox syndrome;levodopa-induced dyskinesia; mental retardation; movement disordersincluding akinesias and akinetic (rigid) syndromes (including basalganglia calcification, corticobasal degeneration, multiple systematrophy, Parkinsonism-ALS dementia complex, Parkinson's disease,postencephalitic parkinsonism, and progressively supranuclear palsy);muscular spasms and disorders associated with muscular spasticity orweakness including chorea (such as benign hereditary chorea,drug-induced chorea, hemiballism, Huntington's disease,neuroacanthocytosis, Sydenham's chorea, and symptomatic chorea),dyskinesia (including tics such as complex tics, simple tics, andsymptomatic tics), myoclonus (including generalized myoclonus and focalcyloclonus), tremor (such as rest tremor, postural tremor, and intentiontremor) and dystonia (including axial dystonia, dystonic writer's cramp,hemiplegic dystonia, paroxysmal dystonia, and focal dystonia such asblepharospasm, oromandibular dystonia, and spasmodic dysphonia andtorticollis); neuronal damage including ocular damage, retinopathy ormacular degeneration of the eye; neurotoxic injury which followscerebral stroke, thromboembolic stroke, hemorrhagic stroke, cerebralischemia, cerebral vasospasm, hypoglycemia, amnesia, hypoxia, anoxia,perinatal asphyxia and cardiac arrest; Parkinson's disease; seizure;status epilecticus; stroke; tinnitus; tubular sclerosis, and viralinfection induced neurodegeneration (e.g., caused by acquiredimmunodeficiency syndrome (AIDS) and encephalopathies).Neurodegenerative diseases also include, but are not limited to,neurotoxic injury which follows cerebral stroke, thromboembolic stroke,hemorrhagic stroke, cerebral ischemia, cerebral vasospasm, hypoglycemia,amnesia, hypoxia, anoxia, perinatal asphyxia and cardiac arrest. Methodsof treating or preventing a neurodegenerative disease also includetreating or preventing loss of neuronal function characteristic ofneurodegenerative disorder.

Mood Disorders

Also provided herein are methods for treating a mood disorder, forexample clinical depression, postnatal depression or postpartumdepression, perinatal depression, atypical depression, melancholicdepression, psychotic major depression, cataonic depression, seasonalaffective disorder, dysthymia, double depression, depressive personalitydisorder, recurrent brief depression, minor depressive disorder, bipolardisorder or manic depressive disorder, depression caused by chronicmedical conditions, treatment-resistant depression, refractorydepression, suicidality, suicidal ideation, or suicidal behavior. Insome embodiments, the method described herein provides therapeuticeffect to a subject suffering from depression (e.g., moderate or severedepression). In some embodiments, the mood disorder is associated with adisease or disorder described herein (e.g., neuroendocrine diseases anddisorders, neurodegenerative diseases and disorders (e.g., epilepsy),movement disorders, tremor (e.g., Parkinson's Disease), women's healthdisorders or conditions).

Clinical depression is also known as major depression, major depressivedisorder (MDD), severe depression, unipolar depression, unipolardisorder, and recurrent depression, and refers to a mental disordercharacterized by pervasive and persistent low mood that is accompaniedby low self-esteem and loss of interest or pleasure in normallyenjoyable activities. Some people with clinical depression have troublesleeping, lose weight, and generally feel agitated and irritable.Clinical depression affects how an individual feels, thinks, and behavesand may lead to a variety of emotional and physical problems.Individuals with clinical depression may have trouble doing day-to-dayactivities and make an individual feel as if life is not worth living.

Peripartum depression refers to depression in pregnancy. Symptomsinclude irritability, crying, feeling restless, trouble sleeping,extreme exhaustion (emotional and/or physical), changes in appetite,difficulty focusing, increased anxiety and/or worry, disconnectedfeeling from baby and/or fetus, and losing interest in formerlypleasurable activities.

Postnatal depression (PND) is also referred to as postpartum depression(PPD), and refers to a type of clinical depression that affects womenafter childbirth. Symptoms can include sadness, fatigue, changes insleeping and eating habits, reduced sexual desire, crying episodes,anxiety, and irritability. In some embodiments, the PND is atreatment-resistant depression (e.g., a treatment-resistant depressionas described herein). In some embodiments, the PND is refractorydepression (e.g., a refractory depression as described herein).

In some embodiments, a subject having PND also experienced depression,or a symptom of depression during pregnancy. This depression is referredto herein as) perinatal depression. In an embodiment, a subjectexperiencing perinatal depression is at increased risk of experiencingPND.

Atypical depression (AD) is characterized by mood reactivity (e.g.,paradoxical anhedonia) and positivity, significant weight gain orincreased appetite. Patients suffering from AD also may have excessivesleep or somnolence (hypersomnia), a sensation of limb heaviness, andsignificant social impairment as a consequence of hypersensitivity toperceived interpersonal rejection.

Melancholic depression is characterized by loss of pleasure (anhedonia)in most or all activities, failures to react to pleasurable stimuli,depressed mood more pronounced than that of grief or loss, excessiveweight loss, or excessive guilt.

Psychotic major depression (PMD) or psychotic depression refers to amajor depressive episode, in particular of melancholic nature, where theindividual experiences psychotic symptoms such as delusions andhallucinations.

Catatonic depression refers to major depression involving disturbancesof motor behavior and other symptoms. An individual may become mute andstuporose, and either is immobile or exhibits purposeless or bizarremovements.

Seasonal affective disorder (SAD) refers to a type of seasonaldepression wherein an individual has seasonal patterns of depressiveepisodes coming on in the fall or winter.

Dysthymia refers to a condition related to unipolar depression, wherethe same physical and cognitive problems are evident. They are not assevere and tend to last longer (e.g., at least 2 years).

Double depression refers to fairly depressed mood (dysthymia) that lastsfor at least 2 years and is punctuated by periods of major depression.

Depressive Personality Disorder (DPD) refers to a personality disorderwith depressive features.

Recurrent Brief Depression (RBD) refers to a condition in whichindividuals have depressive episodes about once per month, each episodelasting 2 weeks or less and typically less than 2-3 days.

Minor depressive disorder or minor depression refers to a depression inwhich at least 2 symptoms are present for 2 weeks.

Bipolar disorder or manic depressive disorder causes extreme mood swingsthat include emotional highs (mania or hypomania) and lows (depression).During periods of mania the individual may feel or act abnormally happy,energetic, or irritable. They often make poorly thought out decisionswith little regard to the consequences. The need for sleep is usuallyreduced. During periods of depression there may be crying, poor eyecontact with others, and a negative outlook on life. The risk of suicideamong those with the disorder is high at greater than 6% over 20 years,while self-harm occurs in 30-40%. Other mental health issues such asanxiety disorder and substance use disorder are commonly associated withbipolar disorder.

Depression caused by chronic medical conditions refers to depressioncaused by chronic medical conditions such as cancer or chronic pain,chemotherapy, chronic stress.

Treatment-resistant depression refers to a condition where theindividuals have been treated for depression, but the symptoms do notimprove. For example, antidepressants or psychological counseling(psychotherapy) do not ease depression symptoms for individuals withtreatment-resistant depression. In some cases, individuals withtreatment-resistant depression improve symptoms, but come back.Refractory depression occurs in patients suffering from depression whoare resistant to standard pharmacological treatments, includingtricyclic antidepressants, MAOIs, SSRIs, and double and triple uptakeinhibitors and/or anxiolytic drugs, as well as non-pharmacologicaltreatments (e.g., psychotherapy, electroconvulsive therapy, vagus nervestimulation and/or transcranial magnetic stimulation).

Post-surgical depression refers to feelings of depression that follow asurgical procedure (e.g., as a result of having to confront one'smortality). For example, individuals may feel sadness or empty moodpersistently, a loss of pleasure or interest in hobbies and activitiesnormally enjoyed, or a persistent felling of worthlessness orhopelessness.

Mood disorder associated with conditions or disorders of women's healthrefers to mood disorders (e.g., depression) associated with (e.g.,resulting from) a condition or disorder of women's health (e.g., asdescribed herein).

Suicidality, suicidal ideation, suicidal behavior refers to the tendencyof an individual to commit suicide. Suicidal ideation concerns thoughtsabout or an unusual preoccupation with suicide. The range of suicidalideation varies greatly, from e.g., fleeting thoughts to extensivethoughts, detailed planning, role playing, incomplete attempts. Symptomsinclude talking about suicide, getting the means to commit suicide,withdrawing from social contact, being preoccupied with death, feelingtrapped or hopeless about a situation, increasing use of alcohol ordrugs, doing risky or self-destructive things, saying goodbye to peopleas if they won't be seen again.

Symptoms of depression include persistent anxious or sad feelings,feelings of helplessness, hopelessness, pessimism, worthlessness, lowenergy, restlessness, difficulty sleeping, sleeplessness, irritability,fatigue, motor challenges, loss of interest in pleasurable activities orhobbies, loss of concentration, loss of energy, poor self-esteem,absence of positive thoughts or plans, excessive sleeping, overeating,appetite loss, insomnia, self-harm, thoughts of suicide, and suicideattempts. The presence, severity, frequency, and duration of symptomsmay vary on a case to case basis. Symptoms of depression, and relief ofthe same, may be ascertained by a physician or psychologist (e.g., by amental state examination).

In some embodiments, the method comprises monitoring a subject with aknown depression scale, e.g., the Hamilton Depression (HAM-D) scale, theClinical Global Impression-Improvement Scale (CGI), and theMontgomery-Åsberg Depression Rating Scale (MADRS). In some embodiments,a therapeutic effect can be determined by reduction in HamiltonDepression (HAM-D) total score exhibited by the subject. Reduction inthe HAM-D total score can happen within 4, 3, 2, or 1 days; or 96, 84,72, 60, 48, 24, 20, 16, 12, 10, 8 hours or less. The therapeutic effectcan be assessed across a specified treatment period. For example, thetherapeutic effect can be determined by a decrease from baseline inHAM-D total score after administering a compound described herein, e.g.,a compound of Formula (I) (e.g., 12, 24, or 48 hours afteradministration; or 24, 48, 72, or 96 hours or more; or 1 day, 2 days, 14days, 21 days, or 28 days; or 1 week, 2 weeks, 3 weeks, or 4 weeks; or 1month, 2 months, 6 months, or 10 months; or 1 year, 2 years, or forlife).

In some embodiments, the subject has a mild depressive disorder, e.g.,mild major depressive disorder. In some embodiments, the subject has amoderate depressive disorder, e.g., moderate major depressive disorder.In some embodiments, the subject has a severe depressive disorder, e.g.,severe major depressive disorder. In some embodiments, the subject has avery severe depressive disorder, e.g., very severe major depressivedisorder. In some embodiments, the baseline HAM-D total score of thesubject (i.e., prior to treatment with a compound described herein,e.g., a compound of Formula (I)) is at least 24. In some embodiments,the baseline HAM-D total score of the subject is at least 18. In someembodiments, the baseline HAM-D total score of the subject is betweenand including 14 and 18. In some embodiments, the baseline HAM-D totalscore of the subject is between and including 19 and 22. In someembodiments, the HAM-D total score of the subject before treatment witha compound described herein, e.g., a compound of Formula (I), is greaterthan or equal to 23. In some embodiments, the baseline score is at least10, 15, or 20. In some embodiments, the HAM-D total score of the subjectafter treatment with a compound described herein, e.g., a compound ofFormula (I), is about 0 to 10 (e.g., less than 10; 0 to 10, 0 to 6, 0 to4, 0 to 3, 0 to 2, or 1.8). In some embodiments, the HAM-D total scoreafter treatment with a compound described herein, e.g., a compound ofFormula (I), is less than 10, 7, 5, or 3. In some embodiments, thedecrease in HAM-D total score is from a baseline score of about 20 to 30(e.g., 22 to 28, 23 to 27, 24 to 27, 25 to 27, 26 to 27) to a HAM-Dtotal score at about 0 to 10 (e.g., less than 10; 0 to 10, 0 to 6, 0 to4, 0 to 3, 0 to 2, or 1.8) after treatment with a compound describedherein, e.g., a compound of Formula (I). In some embodiments, thedecrease in the baseline HAM-D total score to HAM-D total score aftertreatment with a compound described herein, e.g., a compound of Formula(I), is at least 1, 2, 3, 4, 5, 7, 10, 25, 40, 50, or 100 fold). In someembodiments, the percentage decrease in the baseline HAM-D total scoreto HAM-D total score after treatment with a compound described herein,e.g., a compound of Formula (I), is at least 50% (e.g., 60%, 70%, 80%,or 90%). In some embodiments, the therapeutic effect is measured as adecrease in the HAM-D total score after treatment with a compounddescribed herein, e.g., a compound of Formula (I), relative to thebaseline HAM-D total score (e.g., 12, 24, 48 hours after administration;or 24, 48, 72, 96 hours or more; or 1 day, 2 days, 14 days, or more) isat least 10, 15, or 20 points.

In some embodiments, the method of treating a depressive disorder, e.g.,major depressive disorder provides a therapeutic effect (e.g., asmeasured by reduction in Hamilton Depression Score (HAM-D)) within 14,10, 4, 3, 2, or 1 days, or 24, 20, 16, 12, 10, or 8 hours or less. Insome embodiments, the method of treating the depressive disorder, e.g.,major depressive disorder, provides a therapeutic effect (e.g., asdetermined by a statistically significant reduction in HAM-D totalscore) within the first or second day of the treatment with a compounddescribed herein, e.g., a compound of Formula (I). In some embodiments,the method of treating the depressive disorder, e.g., major depressivedisorder, provides a therapeutic effect (e.g., as determined by astatistically significant reduction in HAM-D total score) within lessthan or equal to 14 days since the beginning of the treatment with acompound described herein, e.g., a compound of Formula (I). In someembodiments, the method of treating the depressive disorder, e.g., majordepressive disorder, provides a therapeutic effect (e.g., as determinedby a statistically significant reduction in HAM-D total score) withinless than or equal to 21 days since the beginning of the treatment witha compound described herein, e.g., a compound of Formula (I). In someembodiments, the method of treating the depressive disorder, e.g., majordepressive disorder, provides a therapeutic effect (e.g., as determinedby a statistically significant reduction in HAM-D total score) withinless than or equal to 28 days since the beginning of the treatment witha compound described herein, e.g., a compound of Formula (I). In someembodiments, the therapeutic effect is a decrease from baseline in HAM-Dtotal score after treatment with a compound described herein, e.g., acompound of Formula (I) (e.g., treatment with a compound describedherein, e.g., a compound of Formula (I), once a day for 14 days). Insome embodiments, the HAM-D total score of the subject before treatmentwith a compound described herein, e.g., a compound of Formula (I), is atleast 24. In some embodiments, the HAM-D total score of the subjectbefore treatment with a compound described herein, e.g., a compound ofFormula (I), is at least 18. In some embodiments, the HAM-D total scoreof the subject before treatment with a compound described herein, e.g.,a compound of Formula (I), is between and including 14 and 18. In someembodiments, the decrease in HAM-D total score after treating thesubject with a compound described herein, e.g., a compound of Formula(I), relative to the baseline HAM-D total score is at least 10. In someembodiments, the decrease in HAM-D total score after treating thesubject with a compound described herein, e.g., a compound of Formula(I), relative to the baseline HAM-D total score is at least 15 (e.g., atleast 17). In some embodiments, the HAM-D total score associated withtreating the subject with a compound described herein, e.g., a compoundof Formula (I), is no more than a number ranging from 6 to 8. In someembodiments, the HAM-D total score associated with treating the subjectwith a compound described herein, e.g., a compound of Formula (I), is nomore than 7.

In some embodiments, the method provides therapeutic effect (e.g., asmeasured by reduction in Clinical Global Impression-Improvement Scale(CGI)) within 14, 10, 4, 3, 2, or 1 days, or 24, 20, 16, 12, 10, or 8hours or less. In some embodiments, the CNS-disorder is a depressivedisorder, e.g., major depressive disorder. In some embodiments, themethod of treating the depressive disorder, e.g., major depressivedisorder provides a therapeutic effect within the second day of thetreatment period. In some embodiments, the therapeutic effect is adecrease from baseline in CGI score at the end of a treatment period(e.g., 14 days after administration).

In some embodiments, the method provides therapeutic effect (e.g., asmeasured by reduction in Montgomery-Åsberg Depression Rating Scale(MADRS)) within 14, 10, 4, 3, 2, or 1 days, or 24, 20, 16, 12, 10, or 8hours or less. In some embodiments, the CNS-disorder is a depressivedisorder, e.g., major depressive disorder. In some embodiments, themethod of treating the depressive disorder, e.g., major depressivedisorder provides a therapeutic effect within the second day of thetreatment period. In some embodiments, the therapeutic effect is adecrease from baseline in MADRS score at the end of a treatment period(e.g., 14 days after administration).

A therapeutic effect for major depressive disorder can be determined bya reduction in Montgomery-Åsberg Depression Rating Scale (MADRS) scoreexhibited by the subject. For example, the MADRS score can be reducedwithin 4, 3, 2, or 1 days; or 96, 84, 72, 60, 48, 24, 20, 16, 12, 10, 8hours or less. The Montgomery-Åsberg Depression Rating Scale (MADRS) isa ten-item diagnostic questionnaire (regarding apparent sadness,reported sadness, inner tension, reduced sleep, reduced appetite,concentration difficulties, lassitude, inability to feel, pessimisticthoughts, and suicidal thoughts) which psychiatrists use to measure theseverity of depressive episodes in patients with mood disorders.

In some embodiments, the method provides therapeutic effect (e.g., asmeasured by reduction in Edinburgh Postnatal Depression Scale (EPDS))within 4, 3, 2, 1 days; 24, 20, 16, 12, 10, 8 hours or less. In someembodiments, the therapeutic effect is an improvement measured by theEPDS.

In some embodiments, the method provides therapeutic effect (e.g., asmeasured by reduction in Generalized Anxiety Disorder 7-Item Scale(GAD-7)) within 4, 3, 2, 1 days; 24, 20, 16, 12, 10, 8 hours or less.

Anxiety Disorders

Provided herein are methods for treating anxiety disorders (e.g.,generalized anxiety disorder, panic disorder, obsessive compulsivedisorder, phobia, post-traumatic stress disorder). Anxiety disorder is ablanket term covering several different forms of abnormal andpathological fear and anxiety. Current psychiatric diagnostic criteriarecognize a wide variety of anxiety disorders.

Generalized anxiety disorder is a common chronic disorder characterizedby long-lasting anxiety that is not focused on any one object orsituation. Those suffering from generalized anxiety experiencenon-specific persistent fear and worry and become overly concerned witheveryday matters. Generalized anxiety disorder is the most commonanxiety disorder to affect older adults.

In panic disorder, a person suffers from brief attacks of intense terrorand apprehension, often marked by trembling, shaking, confusion,dizziness, nausea, difficulty breathing. These panic attacks, defined bythe APA as fear or discomfort that abruptly arises and peaks in lessthan ten minutes, can last for several hours and can be triggered bystress, fear, or even exercise; although the specific cause is notalways apparent. In addition to recurrent unexpected panic attacks, adiagnosis of panic disorder also requires that said attacks have chronicconsequences: either worry over the attacks' potential implications,persistent fear of future attacks, or significant changes in behaviorrelated to the attacks. Accordingly, those suffering from panic disorderexperience symptoms even outside of specific panic episodes. Often,normal changes in heartbeat are noticed by a panic sufferer, leadingthem to think something is wrong with their heart or they are about tohave another panic attack. In some cases, a heightened awareness(hypervigilance) of body functioning occurs during panic attacks,wherein any perceived physiological change is interpreted as a possiblelife threatening illness (i.e. extreme hypochondriasis).

Obsessive compulsive disorder is a type of anxiety disorder primarilycharacterized by repetitive obsessions (distressing, persistent, andintrusive thoughts or images) and compulsions (urges to perform specificacts or rituals). The OCD thought pattern may be likened tosuperstitions insofar as it involves a belief in a causativerelationship where, in reality, one does not exist. Often the process isentirely illogical; for example, the compulsion of walking in a certainpattern may be employed to alleviate the obsession of impending harm.And in many cases, the compulsion is entirely inexplicable, simply anurge to complete a ritual triggered by nervousness. In a minority ofcases, sufferers of OCD may only experience obsessions, with no overtcompulsions; a much smaller number of sufferers experience onlycompulsions.

The single largest category of anxiety disorders is that of phobia,which includes all cases in which fear and anxiety is triggered by aspecific stimulus or situation. Sufferers typically anticipateterrifying consequences from encountering the object of their fear,which can be anything from an animal to a location to a bodily fluid.

Post-traumatic stress disorder or PTSD is an anxiety disorder whichresults from a traumatic experience. Post-traumatic stress can resultfrom an extreme situation, such as combat, rape, hostage situations, oreven serious accident. It can also result from long term (chronic)exposure to a severe stressor, for example soldiers who endureindividual battles but cannot cope with continuous combat. Commonsymptoms include flashbacks, avoidant behaviors, and depression.

Women's Health Disorders

Provided herein are methods for treating conditions or disorders relatedto women's health. Conditions or disorders related to women's healthinclude, but are not limited to, gynecological health and disorders(e.g., premenstrual syndrome (PMS), premenstrual dysphoric disorder(PMDD)), pregnancy issues (e.g., miscarriage, abortion), infertility andrelated disorders (e.g., polycystic ovary syndrome (PCOS)), otherdisorders and conditions, and issues related to women's overall healthand wellness (e.g., menopause).

Gynecological health and disorders affecting women include menstruationand menstrual irregularities; urinary tract health, including urinaryincontinence and pelvic floor disorders; and such disorders as bacterialvaginosis, vaginitis, uterine fibroids, and vulvodynia.

Premenstrual syndrome (PMS) refers to physical and emotional symptomsthat occur in the one to two weeks before a women's period. Symptomsvary but can include bleeding, mood swings, tender breasts, foodcravings, fatigue, irritability, acne, and depression.

Premenstrual dysphoric disorder (PMDD) is a severe form of PMS. Thesymptoms of PMDD are similar to PMS but more severe and may interferewith work, social activity, and relationships. PMDD symptoms includemood swings, depressed mood or feelings of hopelessness, marked anger,increased interpersonal conflicts, tension and anxiety, irritability,decreased interest in usual activities, difficulty concentrating,fatigue, change in appetite, feeling out of control or overwhelmed,sleep problems, physical problems (e.g., bloating, breast tenderness,swelling, headaches, joint or muscle pain).

Pregnancy issues include preconception care and prenatal care, pregnancyloss (miscarriage and stillbirth), preterm labor and premature birth,sudden infant death syndrome (SIDS), breastfeeding, and birth defects.

Miscarriage refers to a pregnancy that ends on its own, within the first20 weeks of gestation.

Abortion refers to the deliberate termination of a pregnancy, which canbe performed during the first 28 weeks of pregnancy.

Infertility and related disorders include uterine fibroids, polycysticovary syndrome, endometriosis, and primary ovarian insufficiency.

Polycystic ovary syndrome (PCOS) refers to an endocrine system disorderamong women of reproductive age. PCOS is a set of symptoms resultingfrom an elevated male hormone in women. Most women with PCOS grow manysmall cysts on their ovaries. Symptoms of PCOS include irregular or nomenstrual periods, heavy periods, excess body and facial hair, acne,pelvic pain, difficulty getting pregnant, and patches of thick, darker,velvety skin. PCOS may be associated with conditions including type 2diabetes, obesity, obstructive sleep apnea, heart disease, mooddisorders, and endometrial cancer.

Other disorders and conditions that affect only women include Turnersyndrome, Rett syndrome, and ovarian and cervical cancers.

Issues related to women's overall health and wellness include violenceagainst women, women with disabilities and their unique challenges,osteoporosis and bone health, and menopause.

Menopause refers to the 12 months after a woman's last menstrual periodand marks the end of menstrual cycles. Menopause typically occurs in awoman's 40s or 50s. Physical symptoms such as hot flashes and emotionalsymptoms of menopause may disrupt sleep, lower energy, or triggeranxiety or feelings of sadness or loss. Menopause includes naturalmenopause and surgical menopause, which is a type of induced menopausedue to an event such as surgery (e.g., hysterectomy, oophorectomy;cancer). It is induced when the ovaries are gravely damaged by, e.g.,radiation, chemotherapy, or other medications.

Epilepsy

The compound of Formula (I), or pharmaceutically acceptable salt, or apharmaceutically acceptable composition thereof, can be used in a methoddescribed herein, for example in the treatment of a disorder describedherein such as epilepsy, status epilepticus, or seizure.

Epilepsy is a brain disorder characterized by repeated seizures overtime. Types of epilepsy can include, but are not limited to generalizedepilepsy, e.g., childhood absence epilepsy, juvenile nyoclonic epilepsy,epilepsy with grand-mal seizures on awakening, West syndrome,Lennox-Gastaut syndrome, partial epilepsy, e.g., temporal lobe epilepsy,frontal lobe epilepsy, benign focal epilepsy of childhood.

Epileptogenesis

The compounds and methods described herein can be used to treat orprevent epileptogenesis. Epileptogenesis is a gradual process by which anormal brain develops epilepsy (a chronic condition in which seizuresoccur). Epileptogenesis results from neuronal damage precipitated by theinitial insult (e.g., status epilepticus).

Status Epilepticus (SE)

Status epilepticus (SE) can include, e.g., convulsive statusepilepticus, e.g., early status epilepticus, established statusepilepticus, refractory status epilepticus, super-refractory statusepilepticus; non-convulsive status epilepticus, e.g., generalized statusepilepticus, complex partial status epilepticus; generalized periodicepileptiform discharges; and periodic lateralized epileptiformdischarges. Convulsive status epilepticus is characterized by thepresence of convulsive status epileptic seizures, and can include earlystatus epilepticus, established status epilepticus, refractory statusepilepticus, super-refractory status epilepticus. Early statusepilepticus is treated with a first line therapy. Established statusepilepticus is characterized by status epileptic seizures which persistdespite treatment with a first line therapy, and a second line therapyis administered. Refractory status epilepticus is characterized bystatus epileptic seizures which persist despite treatment with a firstline and a second line therapy, and a general anesthetic is generallyadministered. Super refractory status epilepticus is characterized bystatus epileptic seizures which persist despite treatment with a firstline therapy, a second line therapy, and a general anesthetic for 24hours or more.

Non-convulsive status epilepticus can include, e.g., focalnon-convulsive status epilepticus, e.g., complex partial non-convulsivestatus epilepticus, simple partial non-convulsive status epilepticus,subtle non-convulsive status epilepticus; generalized non-convulsivestatus epilepticus, e.g., late onset absence non-convulsive statusepilepticus, atypical absence non-convulsive status epilepticus, ortypical absence non-convulsive status epilepticus.

The compound of Formula (I) or pharmaceutically acceptable salt, or apharmaceutically acceptable composition thereof, can also beadministered as a prophylactic to a subject having a CNS disorder e.g.,a traumatic brain injury, status epilepticus, e.g., convulsive statusepilepticus, e.g., early status epilepticus, established statusepilepticus, refractory status epilepticus, super-refractory statusepilepticus; non-convulsive status epilepticus, e.g., generalized statusepilepticus, complex partial status epilepticus; generalized periodicepileptiform discharges; and periodic lateralized epileptiformdischarges; prior to the onset of a seizure.

Seizure

A seizure is the physical findings or changes in behavior that occurafter an episode of abnormal electrical activity in the brain. The term“seizure” is often used interchangeably with “convulsion.” Convulsionsare when a person's body shakes rapidly and uncontrollably. Duringconvulsions, the person's muscles contract and relax repeatedly.

Based on the type of behavior and brain activity, seizures are dividedinto two broad categories: generalized and partial (also called local orfocal). Classifying the type of seizure helps doctors diagnose whetheror not a patient has epilepsy.

Generalized seizures are produced by electrical impulses from throughoutthe entire brain, whereas partial seizures are produced (at leastinitially) by electrical impulses in a relatively small part of thebrain. The part of the brain generating the seizures is sometimes calledthe focus.

There are six types of generalized seizures. The most common anddramatic, and therefore the most well-known, is the generalizedconvulsion, also called the grand-mal seizure. In this type of seizure,the patient loses consciousness and usually collapses. The loss ofconsciousness is followed by generalized body stiffening (called the“tonic” phase of the seizure) for 30 to 60 seconds, then by violentjerking (the “clonic” phase) for 30 to 60 seconds, after which thepatient goes into a deep sleep (the “postictal” or after-seizure phase).During grand-mal seizures, injuries and accidents may occur, such astongue biting and urinary incontinence.

Absence seizures cause a short loss of consciousness (just a fewseconds) with few or no symptoms. The patient, most often a child,typically interrupts an activity and stares blankly. These seizuresbegin and end abruptly and may occur several times a day. Patients areusually not aware that they are having a seizure, except that they maybe aware of “losing time.”

Myoclonic seizures consist of sporadic jerks, usually on both sides ofthe body. Patients sometimes describe the jerks as brief electricalshocks. When violent, these seizures may result in dropping orinvoluntarily throwing objects.

Clonic seizures are repetitive, rhythmic jerks that involve both sidesof the body at the same time.

Tonic seizures are characterized by stiffening of the muscles.

Atonic seizures consist of a sudden and general loss of muscle tone,particularly in the arms and legs, which often results in a fall.

Seizures described herein can include epileptic seizures; acuterepetitive seizures; cluster seizures; continuous seizures; unremittingseizures; prolonged seizures; recurrent seizures; status epilepticusseizures, e.g., refractory convulsive status epilepticus, non-convulsivestatus epilepticus seizures; refractory seizures; myoclonic seizures;tonic seizures; tonic-clonic seizures; simple partial seizures; complexpartial seizures; secondarily generalized seizures; atypical absenceseizures; absence seizures; atonic seizures; benign Rolandic seizures;febrile seizures; emotional seizures; focal seizures; gelastic seizures;generalized onset seizures; infantile spasms; Jacksonian seizures;massive bilateral myoclonus seizures; multifocal seizures; neonatalonset seizures; nocturnal seizures; occipital lobe seizures; posttraumatic seizures; subtle seizures; Sylvan seizures; visual reflexseizures; or withdrawal seizures. In some embodiments, the seizure is ageneralized seizure associated with Dravet Syndrome, Lennox-GastautSyndrome, Tuberous Sclerosis Complex, Rett Syndrome or PCDH19 FemalePediatric Epilepsy.

Movement Disorders

Also described herein are methods for treating a movement disorder. Asused herein, “movement disorders” refers to a variety of diseases anddisorders that are associated with hyperkinetic movement disorders andrelated abnormalities in muscle control. Exemplary movement disordersinclude, but are not limited to, Parkinson's disease and parkinsonism(defined particularly by bradykinesia), dystonia, chorea andHuntington's disease, ataxia, tremor (e.g., essential tremor), myoclonusand startle, tics and Tourette syndrome, Restless legs syndrome, stiffperson syndrome, and gait disorders.

Tremor

The methods described herein can be used to treat tremor, for examplethe compound of Formula (I) can be used to treat cerebellar tremor orintention tremor, dystonic tremor, essential tremor, orthostatic tremor,parkinsonian tremor, physiological tremor, psychogenic tremor, or rubraltremor. Tremor includes hereditary, degenerative, and idiopathicdisorders such as Wilson's disease, Parkinson's disease, and essentialtremor, respectively; metabolic diseases (e.g., thyroid-parathyroid-,liver disease and hypoglycemia); peripheral neuropathies (associatedwith Charcot-Marie-Tooth, Roussy-Levy, diabetes mellitus, complexregional pain syndrome); toxins (nicotine, mercury, lead, CO, Manganese,arsenic, toluene); drug-induced (narcoleptics, tricyclics, lithium,cocaine, alcohol, adrenaline, bronchodilators, theophylline, caffeine,steroids, valproate, amiodarone, thyroid hormones, vincristine); andpsychogenic disorders. Clinical tremor can be classified intophysiologic tremor, enhanced physiologic tremor, essential tremorsyndromes (including classical essential tremor, primary orthostatictremor, and task- and position-specific tremor), dystonic tremor,parkinsonian tremor, cerebellar tremor, Holmes' tremor (i.e., rubraltremor), palatal tremor, neuropathic tremor, toxic or drug-inducedtremor, and psychogenic tremor.

Tremor is an involuntary, at times rhythmic, muscle contraction andrelaxation that can involve oscillations or twitching of one or morebody parts (e.g., hands, arms, eyes, face, head, vocal folds, trunk,legs).

Cerebellar tremor or intention tremor is a slow, broad tremor of theextremities that occurs after a purposeful movement. Cerebellar tremoris caused by lesions in or damage to the cerebellum resulting from,e.g., tumor, stroke, disease (e.g., multiple sclerosis, an inheriteddegenerative disorder).

Dystonic tremor occurs in individuals affected by dystonia, a movementdisorder in which sustained involuntary muscle contractions causetwisting and repetitive motions and/or painful and abnormal postures orpositions. Dystonic tremor may affect any muscle in the body. Dystonictremors occurs irregularly and often can be relieved by complete rest.

Essential tremor or benign essential tremor is the most common type oftremor. Essential tremor may be mild and nonprogressive in some, and maybe slowly progressive, starting on one side of the body but affect bothsides within 3 years. The hands are most often affected, but the head,voice, tongue, legs, and trunk may also be involved. Tremor frequencymay decrease as the person ages, but severity may increase. Heightenedemotion, stress, fever, physical exhaustion, or low blood sugar maytrigger tremors and/or increase their severity. Symptoms generallyevolve over time and can be both visible and persistent following onset.

Orthostatic tremor is characterized by fast (e.g., greater than 12 Hz)rhythmic muscle contractions that occurs in the legs and trunkimmediately after standing. Cramps are felt in the thighs and legs andthe patient may shake uncontrollably when asked to stand in one spot.Orthostatic tremor may occurs in patients with essential tremor.

Parkinsonian tremor is caused by damage to structures within the brainthat control movement. Parkinsonian tremor is often a precursor toParkinson's disease and is typically seen as a “pill-rolling” action ofthe hands that may also affect the chin, lips, legs, and trunk. Onset ofparkinsonian tremor typically begins after age 60. Movement starts inone limb or on one side of the body and can progress to include theother side.

Physiological tremor can occur in normal individuals and have noclinical significance. It can be seen in all voluntary muscle groups.Physiological tremor can be caused by certain drugs, alcohol withdrawal,or medical conditions including an overactive thyroid and hypoglycemia.The tremor classically has a frequency of about 10 Hz.

Psychogenic tremor or hysterical tremor can occur at rest or duringpostural or kinetic movement. Patient with psychogenic tremor may have aconversion disorder or another psychiatric disease.

Rubral tremor is characterized by coarse slow tremor which can bepresent at rest, at posture, and with intention. The tremor isassociated with conditions that affect the red nucleus in the midbrain,classical unusual strokes.

Parkinson's Disease affects nerve cells in the brain that producedopamine. Symptoms include muscle rigidity, tremors, and changes inspeech and gait. Parkinsonism is characterized by tremor, bradykinesia,rigidity, and postural instability. Parkinsonism shares symptoms foundin Parkinson's Disease, but is a symptom complex rather than aprogressive neurodegenerative disease.

Dystonia is a movement disorder characterized by sustained orintermittent muscle contractions causing abnormal, often repetitivemovements or postures. Dystonic movements can be patterned, twisting,and may be tremulous. Dystonia is often initiated or worsened byvoluntary action and associated with overflow muscle activation.

Chorea is a neurological disorder characterized by jerky involuntarymovements typically affecting the shoulders, hips, and face.Huntington's Disease is an inherited disease that causes nerve cells inthe brain to waste away. Symptoms include uncontrolled movements,clumsiness, and balance problems. Huntington's disease can hinder walk,talk, and swallowing.

Ataxia refers to the loss of full control of bodily movements, and mayaffect the fingers, hands, arms, legs, body, speech, and eye movements.

Myloclonus and Startle is a response to a sudden and unexpectedstimulus, which can be acoustic, tactile, visual, or vestibular.

Tics are an involuntary movement usually onset suddenly, brief,repetitive, but non-rhythmical, typically imitating normal behavior andoften occurring out of a background of normal activity. Tics can beclassified as motor or vocal, motor tics associated with movements whilevocal tics associated with sound. Tics can be characterized as simple orcomplex. For example simple motor tics involve only a few musclesrestricted to a specific body part. Tourette Syndrome is an inheritedneuropsychiatric disorder with onset in childhood, characterized bymultiple motor tics and at least one vocal tic.

Restless Legs Syndrome is a neurologic sensorimotor disordercharacterized by an overwhelming urge to move the legs when at rest.

Stiff Person Syndrome is a progressive movement disorder characterizedby involuntary painful spasms and rigidity of muscles, usually involvingthe lower back and legs. Stiff-legged gait with exaggerated lumbarhyperlordosis typically results. Characteristic abnormality on EMGrecordings with continuous motor unit activity of the paraspinal axialmuscles is typically observed. Variants include “stiff-limb syndrome”producing focal stiffness typically affecting distal legs and feet.

Gait disorders refer to an abnormality in the manner or style ofwalking, which results from neuromuscular, arthritic, or other bodychanges. Gait is classified according to the system responsible forabnormal locomotion, and include hemiplegic gait, diplegic gait,neuropathic gait, myopathic gait, parkinsonian gait, choreiform gait,ataxic gait, and sensory gait.

Anesthesia/Sedation

Anesthesia is a pharmacologically induced and reversible state ofamnesia, analgesia, loss of responsiveness, loss of skeletal musclereflexes, decreased stress response, or all of these simultaneously.These effects can be obtained from a single drug which alone providesthe correct combination of effects, or occasionally with a combinationof drugs (e.g., hypnotics, sedatives, paralytics, analgesics) to achievevery specific combinations of results. Anesthesia allows patients toundergo surgery and other procedures without the distress and pain theywould otherwise experience.

Sedation is the reduction of irritability or agitation by administrationof a pharmacological agent, generally to facilitate a medical procedureor diagnostic procedure.

Sedation and analgesia include a continuum of states of consciousnessranging from minimal sedation (anxiolysis) to general anesthesia.

Minimal sedation is also known as anxiolysis. Minimal sedation is adrug-induced state during which the patient responds normally to verbalcommands. Cognitive function and coordination may be impaired.Ventilatory and cardiovascular functions are typically unaffected.

Moderate sedation/analgesia (conscious sedation) is a drug-induceddepression of consciousness during which the patient respondspurposefully to verbal command, either alone or accompanied by lighttactile stimulation. No interventions are usually necessary to maintaina patent airway. Spontaneous ventilation is typically adequate.Cardiovascular function is usually maintained.

Deep sedation/analgesia is a drug-induced depression of consciousnessduring which the patient cannot be easily aroused, but respondspurposefully (not a reflex withdrawal from a painful stimulus) followingrepeated or painful stimulation. Independent ventilatory function may beimpaired and the patient may require assistance to maintain a patentairway. Spontaneous ventilation may be inadequate. Cardiovascularfunction is usually maintained.

General anesthesia is a drug-induced loss of consciousness during whichthe patient is not arousable, even to painful stimuli. The ability tomaintain independent ventilatory function is often impaired andassistance is often required to maintain a patent airway. Positivepressure ventilation may be required due to depressed spontaneousventilation or drug-induced depression of neuromuscular function.Cardiovascular function may be impaired.

Sedation in the intensive care unit (ICU) allows the depression ofpatients' awareness of the environment and reduction of their responseto external stimulation. It can play a role in the care of thecritically ill patient, and encompasses a wide spectrum of symptomcontrol that will vary between patients, and among individualsthroughout the course of their illnesses. Heavy sedation in criticalcare has been used to facilitate endotracheal tube tolerance andventilator synchronization, often with neuromuscular blocking agents.

In some embodiments, sedation (e.g., long-term sedation, continuoussedation) is induced and maintained in the ICU for a prolonged period oftime (e.g., 1 day, 2 days, 3 days, 5 days, 1 week, 2 week, 3 weeks, 1month, 2 months). Long-term sedation agents may have long duration ofaction. Sedation agents in the ICU may have short elimination half-life.

Procedural sedation and analgesia, also referred to as conscioussedation, is a technique of administering sedatives or dissociativeagents with or without analgesics to induce a state that allows asubject to tolerate unpleasant procedures while maintainingcardiorespiratory function.

EXAMPLES

In order that the invention described herein may be more fullyunderstood, the following examples are set forth. The synthetic andbiological examples described in this application are offered toillustrate the compounds, pharmaceutical compositions, and methodsprovided herein and are not to be construed in any way as limiting theirscope.

Materials and Methods

The compounds provided herein can be prepared from readily availablestarting materials using the following general methods and procedures.It will be appreciated that where typical or preferred processconditions (i.e., reaction temperatures, times, mole ratios ofreactants, solvents, pressures, etc.) are given, other processconditions can also be used unless otherwise stated. Optimum reactionconditions may vary with the particular reactants or solvent used, butsuch conditions can be determined by one skilled in the art by routineoptimization.

Additionally, as will be apparent to those skilled in the art,conventional protecting groups may be necessary to prevent certainfunctional groups from undergoing undesired reactions. The choice of asuitable protecting group for a particular functional group as well assuitable conditions for protection and deprotection are well known inthe art. For example, numerous protecting groups, and their introductionand removal, are described in T. W. Greene and P. G. M. Wuts, ProtectingGroups in Organic Synthesis, Second Edition, Wiley, New York, 1991, andreferences cited therein.

The compounds provided herein may be isolated and purified by knownstandard procedures. Such procedures include (but are not limited to)recrystallization, column chromatography, HPLC, or supercritical fluidchromatography (SFC). The following schemes are presented with detailsas to the preparation of representative oxysterols that have been listedherein. The compounds provided herein may be prepared from known orcommercially available starting materials and reagents by one skilled inthe art of organic synthesis. Exemplary chiral columns available for usein the separation/purification of the enantiomers/diastereomers providedherein include, but are not limited to, CHIRALPAK® AD-10, CHIRALCEL® OB,CHIRALCEL® OB—H, CHIRALCEL® OD, CHIRALCEL® OD-H, CHIRALCEL® OF,CHIRALCEL® OG, CHIRALCEL® OJ and CHIRALCEL® OK.

¹H-NMR reported herein (e.g., for the region between δ (ppm) of about0.5 to about 4 ppm) will be understood to be an exemplary interpretationof the NMR spectrum (e.g., exemplary peak integratations) of a compound.

Exemplary general method for LCMS/LC ELSD: 30-90AB_2 min. Lcm. (MobilePhase: 1.5 mL/4 L TFA in water (solvent A) and 0.75 mL/4 L TFA inacetonitrile (solvent B), using the elution gradient 30%-90% (solvent B)over 0.9 minutes and holding at 90% for 0.6 minutes at a flow rate of1.2 mL/min; Column: Xtimate C18 2.1*30 mm, 3 μm; Wavelength: UV 220 nm;Column temperature: 50° C.; MS ionization: ESI; Detector: PDA&ELSD)

Abbreviations: PE: petroleum ether; EtOAc: ethyl acetate; THF:tetrahydrofuran; PCC: pyridinium chlorochromate; TLC: thin layerchromatography; PCC: pyridinium chlorochromate; t-BuOK: potassiumtert-butoxide; 9-BBN: 9-borabicyclo[3.3.1]nonane; Pd(t-Bu₃P)₂:bis(tri-tert-butylphosphine)palladium(0); AcCl: acetyl chloride;i-PrMgCl: Isopropylmagnesium chloride; TBSCl:tert-Butyl(chloro)dimethylsilane; (i-PrO)₄Ti: titaniumtetraisopropoxide; BHT: 2,6-di-t-butyl-4-methylphenoxide; Me: methyl;i-Pr: iso-propyl; t-Bu: tert-butyl; Ph: phenyl; Et: ethyl; Bz: benzoyl;BzCl: benzoyl chloride; CsF: cesium fluoride; DCC:dicyclohexylcarbodiimide; DCM: dichloromethane; DMAP:4-dimethylaminopyridine; DMP: Dess-Martin periodinane; EtMgBr:ethylmagnesium bromide; EtOAc: ethyl acetate; TEA: triethylamine; AlaOH:alanine; Boc: t-butoxycarbonyl. Py: pyridine; TBAF:tetra-n-butylammonium fluoride; THF: tetrahydrofuran; TBS:t-butyldimethylsilyl; TMS: trimethylsilyl; TMSCF₃:(Trifluoromethyl)trimethylsilane; Ts: p-toluenesulfonyl; Bu: butyl;Ti(OiPr)₄: tetraisopropoxytitanium; LAH: Lithium Aluminium Hydride; LDA:lithium diisopropylamide; LiOH.H₂O: lithium hydroxide hydrates; MAD:methyl aluminum bis(2,6-di-t-butyl-4-methylphenoxide); MeCN:acetonitrile; NBS: N-bromosuccinimide; Na₂SO₄: sodium sulfate; Na₂S₂O₃:sodium thiosulfate; MeCN: acetonitrile; MeOH: methanol; Boc:t-butoxycarbonyl; MTBE: methyl tert-butyl ether; K-selectride: Potassiumtri(s-butyl)borohydride; 9-BBNdimer: 9-borabicyclo(3.3.1)nonane(dimer);DIPEA: diisopropylethylamine; DMF: dimethylformamide; FA: formic acid;SM: starting material.

Example 1: Synthesis of1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)piperidin-2-one(A7)

Synthesis of A2

To a solution of chloro(methoxymethyl)triphenylphosphorane (35.3 g, 103mmol, 3.0 eq) in THF (100 mL) was added t-BuLi (79.2 mL, 103 mmol, 1.3 Min n-hexane, 3.0 eq) at 0° C. After stirring at 0° C. for 1 h, themixture was added in three portions to A1 (10 g, 34.4 mmol, 1.0 eq) inTHF (100 mL). After warming slowly to rt over 12 h, the mixture wastreated with NH₄Cl (200 mL, 10%) and extracted with ethyl acetate (3×200mL). The combined organic solution was washed with brine (300 mL), driedover Na₂SO₄, filtered and concentrated under vacuum to give an oil,which was purified by flash column (0˜20% of EtOAc in PE) to give A2(6.5 g, 59%) as an oil.

¹H NMR (400 MHz, CDCl3) δ 5.72-5.68 (t, J=2 Hz, 1H), 3.44 (s, 3H),2.36-2.23 (m, 2H), 2.17-2.07 (m, 1H), 1.92-1.74 (m, 3H), 1.71-1.59 (m,3H), 1.51-1.35 (m, 7H), 1.34-1.23 (m, 6H), 1.22-1.01 (m, 5H), 0.86 (s,3H).

Synthesis of A3

To a solution A2 (3 g, 9.41 mmol) in acetone (50 mL) was added p-TsOH(1.75 g, 9.41 mmol). After stirring at 25° C. for 2 h, the reaction wasquenched with water (50 mL) and extracted with EtOAc (3×50 mL). Thecombined organics were washed with NaHCO₃ (100 mL, 10%) and brine (100mL) and dried over Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜20% of EtOAc in PE) to give A3 (2.8 g) as anoil.

¹H NMR (400 MHz, CDCl3) δ 9.78-9.74 (m, 1H), 2.34-2.23 (m, 1H),2.18-2.08 (m, 1H), 2.02-1.95 (m, 1H), 1.88-1.61 (m, 9H), 1.49-1.38 (m,6H), 1.28-1.21 (m, 6H), 1.15-1.06 (m, 3H), 0.94-0.89 (m, 1H), 0.78-0.71(m, 3H).

Synthesis of A4

To a solution of A3 (1.5 g, 4.92 mmol) in toluene (20 mL) was addedphenylmethanamine (1.57 g, 14.7 mmol) and 4-methylbenzenesulfonic acid(137 mg, 0.73 mmol) at 25° C. under N₂. After refluxing for 3 h, thereaction mixture was cooled to 25° C. and a suspension of NaBH₄ (556 mg,14.7 mmol) in MeOH (20 mL) was added. After stirring for 1 h, themixture was poured into water (20 mL) and extracted with EtOAc (3×20mL). The combined organic solution was washed with NaHCO₃ (30 mL, 10%)and brine (20 mL), dried over Na₂SO₄, filtered and concentrated invacuum. The residue was purified by flash column (0˜20% of EtOAc in PE,0.5% of NH₃.H₂O in PE) to give A4 (1 g, 51%) as a solid.

¹H NMR (400 MHz, CDCl3) δ 7.32 (s, 2H), 7.31 (s, 2H), 7.25-7.22 (m, 1H),3.87 (s, 1H), 3.78 (s, 2H), 2.76-2.69 (m, 1H), 2.49-2.42 (m, 1H),1.93-1.73 (m, 5H), 1.68-1.63 (m, 2H), 1.49-1.35 (m, 6H), 1.34-1.23 (m,8H), 1.19-0.98 (m, 7H), 0.58 (s, 3H); LC-ELSD/MS purity 90%, MS ESIcalcd. for C₂₇H₄₂NO [M+H]⁺ 396, found 396.

Synthesis of A5

To a solution of A4 (1 g, 2.52 mmol) in EtOAc (20 mL) was added Pd/C(wet, 10%, 0.45 g) under N₂. The suspension was degassed under vacuumand purged with H₂ for three times. After stirring under H₂ (15 psi) at25° C. for 12 h, the reaction mixture was filtered through a pad ofCelite and washed with EtOAc (3×20 mL). The filtrate was concentrated togive a product (900 mg), which need further hydrogenation. To a solutionof the material (900 mg, 2.27 mmol) in EtOAc/MeOH (10 mL/10 mL) wasadded Pd/C (wet, 10%, 408 mg) under N₂. The suspension was degassedunder vacuum and purged with H₂ for three times. After stirring under H₂(15 psi) at 25° C. for 12 h, the reaction mixture was filtered through apad of Celite and washed with EtOAc (3×20 mL). The filtrate wasconcentrated to give a A5 (650 mg) as a solid, which was used withoutfurther purification.

¹H NMR (400 MHz, CDCl3) δ 2.86-2.78 (m, 1H), 2.57-2.45 (m, 1H), 1.79 (s,5H), 1.68-1.61 (m, 4H), 1.49-1.36 (m, 8H), 1.28-1.23 (m, 5H), 1.17-1.01(m, 8H), 0.60 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₀H₃₆NO[M+H]⁺ 306, found 306.

Synthesis of A6

To a solution of A5 (150 mg, 0.490 mmol) and DIPEA (189 mg, 1.47 mmol)in DCM (2 mL) was added 5-chloropentanoyl chloride (91.1 mg, 0.588mmol). The mixture was stirred at 30° C. for 1 hr. The reaction mixturewas quenched with water (2 mL) and extracted with DCM (3×2 mL). Thecombined organic layer was dried over anhydrous sodium sulfate, filteredand concentrated. The residue was purified by column chromatography onsilica gel (10%˜50% of EtOAc in PE) to give A6 (120 mg, 58%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 3.57-3.53 (m, 2H), 3.38-3.30 (m, 1H),3.20-3.10 (m, 1H), 2.22-2.15 (m, 2H), 1.87-1.75 (m, 10H), 1.69-1.61 (m,4H), 1.54-1.38 (m, 8H), 1.35-1.27 (m, 4H), 1.18-1.02 (m, 7H), 0.66 (s,3H).

Synthesis of A7

To a solution of A6 (70 mg, 0.165 mmol) in anhydrous DMF (2 mL) wasadded NaH (60%, 32.8 mg, 0.825 mmol). After stirring at 30° C. for 18 h,the reaction mixture was quenched with ice-water (10 mL) and extractedwith EtOAc (2×10 mL). The combined organic solution was washed with 3%LiCl aqueous (2×10 mL) and brine (10 mL), dried over anhydrous sodiumsulfate, filtered and concentrated. The residue was purified by columnchromatography on silica gel (10%˜80% of EtOAc in PE, basified byaqueous ammonia) to give A7 as an oil. The oil was dissolved in MeCN (2mL), diluted with deionized water (15 mL), concentrated and lyophilizedto give A7 (29 mg, 45%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 3.57-3.48 (m, 1H), 3.32-3.18 (m, 3H), 2.35 (t,J=6.0 Hz, 2H), 1.89-1.69 (m, 10H), 1.68-1.62 (m, 2H), 1.48-1.36 (m, 7H),1.35-1.21 (m, 7H), 1.16-0.98 (m, 6H), 0.70 (s, 3H); LC-ELSD/MS purity99%, MS ESI calcd. for C₂₅H₄₂NO₂[M+H]⁺ 388, found 388.

Example 2: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-N-methylbenzamide(A9)

Synthesis of A8

To a solution of A3 (200 mg, 0.65 mmol) in toluene (10 mL) was addedmethanamine (0.65 mL, 1.31 mmol, 2 M in THF) and 4-methylbenzenesulfonicacid (18.2 mg, 0.098 mmol) at 25° C. under N₂. After refluxing at 110°C. for 3 h, the reaction mixture was cooled to 25° C. and a suspensionof NaBH₄ (74.1 mg, 1.96 mmol) in MeOH (10 mL) was added. After stirringat 25° C. for 1 h, the mixture was poured into water (20 mL) andextracted with EtOAc (3×20 mL). The combined organic solution was washedwith NaHCO₃ (30 mL, 10%, aqueous) and brine (20 mL), dried over Na₂SO₄,filtered and concentrated in vacuum. The residue was purified by flashcolumn (0˜80% of EtOAc in PE, 0.5% NH₃.H₂O in PE) to give A8 (160 mg) asan oil.

LC-ELSD/MS purity 95%, MS ESI calcd. for C₂₁H₃₈NO [M+H]⁺ 320, found 320.

Synthesis of A9

To a solution of benzoic acid (122 mg, 1 mmol) in DCM (3 mL) was addedHATU (285 mg, 0.75 mmol) and Et₃N (252 mg, 2.5 mmol) at 25° C. Afterstirring at 25° C. for 0.5 h, A8 (160 mg, 0.5 mmol) was added. Afterstirring at 25° C. for 10 h, the residue was diluted with water (10 mL)and then extracted with EtOAc (2×10 mL). The combined organic solutionwas washed with water (2×10 mL) and brine (10 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜50% of EtoAc in PE, 0.1% of NH₃.H₂O in PE) to give A9 (130 mg)as a solid, which was purified by HPLC (column: Xtimate C18 150*25 mm*5um), condition: water (0.225% FA)-ACN, gradient: 78-100% B, GradientTime: 7 mins, 100% B Hold Time: 1 min, flow rate: 25 mL/min) to give A9(38 mg, 18%) as a solid.

¹H NMR (400 MHz, CDCl3) δ 7.42-1.33 (m, 5H), 3.78-3.65 (m, 0.6H),3.51-3.38 (m, 1.3H), 3.07 (s, 1.5H), 2.92 (s, 1.5H), 1.92-1.76 (m, 5H),1.65-1.53 (m, 9H), 1.44-1.36 (m, 4H), 1.29-1.22 (m, 5H), 1.13-0.85 (m,5H), 0.76 (s, 1.6H), 0.32 (s, 1.3H); LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₂NO₂[M+H]⁺ 424, found 424.

Example 3: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)benzamide(A10)

To a solution of benzoic acid (158 mg, 1.3 mmol) in DCM (3 mL) was addedHATU (494 mg, 1.3 mmol) and Et₃N (330 mg, 3.27 mmol) at 25° C. Afterstirring for 0.5 h, A5 (200 mg, 0.65 mmol) was added to the reactionmixture. After stirring for 10 h, the mixture was treated by water (10mL) and extracted with EtOAc (2×10 mL). The combined organic solutionwas concentrated under vacuum. The residual was resolved in EtOAc andwashed with water (2×10 mL), brine (10 mL), dried over anhydrous Na₂SO₄,filtered and concentrated to give a solid (150 mg). The solid waspurified by HPLC (column: Xtimate C18 150*25 mm*5 um), condition: water(0.225% FA)-ACN, gradient: 63-93% B, Gradient Time: 7 mins, 100% B HoldTime: 2 min, flow rate: 25 mL/min) to give A10 (6 mg, 4%) as a solid.

¹H NMR (400 MHz, CDCl3) δ 7.77-7.71 (m, 2H), 7.53-7.46 (m, 1H),7.45-7.39 (m, 2H), 5.99 (s, 1H), 3.61-3.51 (m, 1H), 3.42-3.31 (m, 1H),2.01-1.79 (m, 5H), 1.69-1.63 (m, 4H), 1.49-1.29 (m, 10H), 1.26 (s, 3H),1.22-1.04 (m, 6H), 0.72 (m, 3H); LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₇H₄₀NO₂ [M+H]⁺ 410, found 410.

Example 4: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)benzenesulfonamide(A11)

To a solution of A5 (300 mg, 0.9819 mmol) in DCM (10 mL) was added Et₃N(247 mg, 2.45 mmol) and benzenesulfonyl chloride (259 mg, 1.47 mmol) at20° C. After stirring 16 h at 20° C., the reaction mixture was washedwith water (3×100 mL). The combined organic solution was dried overNa₂SO₄, filtered and concentrated to give desired product, which waspurified by combi flash (0-15% of EtOAc in PE) to give A11 (180 mg, 41%)as a solid.

¹H NMR (400 MHz, CDCl₃) δ 7.95-7.85 (m, 2H), 7.65-7.51 (m, 3H),4.31-4.15 (m, 1H), 3.11-3.00 (m, 1H), 2.85-2.75 (m, 1H), 1.91-1.75 (m,5H), 1.74-1.59 (m, 3H), 1.45-1.28 (m, 9H), 1.26 (s, 3H), 1.23-0.91 (m,8H), 0.55 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₆H₃₈NO₂S[M+H—H₂O]⁺428, found 428.

Example 5: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-N-methylbenzenesulfonamide(A12)

To a solution of A11 (142 mg, 0.3186 mmol) in DMF (5 mL) was addedCs₂CO₃ (207 mg, 0.6372 mmol) at 20° C. After stirring for 20 mins, Mel(70 mg, 0.4929 mmol) was added. After stirring for 16 h at 20° C., thereaction mixture was added into water (50 mL) and extracted with EtOAc(2×50 mL). The combined organic solution was washed by water (3×100 mL),dried over Na₂SO₄, filtered and concentrated to give desired product,which was purified by combi-flash (0-15% of EtOAc in PE) to give A12 (64mg, 44%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 7.81-7.75 (m, 2H), 7.55-7.52 (m, 3H),3.11-3.05 (m, 1H), 2.85-2.75 (m, 1H), 2.69 (s, 3H), 1.91-1.59 (m, 10H),1.51-1.28 (m, 9H), 1.26 (s, 3H), 1.24-1.01 (m, 6H), 0.71 (s, 3H);LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₂NO₃S[M+H]⁺ 460, found460.

Examples 6 & 7: Synthesis of(S)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-6-methylpiperidin-2-one(A14) &(R)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-6-methylpiperidin-2-one(A15)

Synthesis of A13

To a solution of A5 (300 mg, 0.981 mmol) in toluene (10 mL) was addedmethyl 5-oxohexanoate (282 mg, 1.96 mmol). After stirring at 120° C. for16 h, the reaction was cooled to 25° C. and MeOH (20 ml) and boranesodium hydride (92.6 mg, 2.45 mmol) were added. After 30 min, themixture was poured into ice-water (50 mL) and extracted with EtOAc (2×30mL). The combined organic solution was washed with brine (50 mL), driedover Na₂SO₄, filtered and concentrated in vacuum. The residue waspurified by a silica gel column (PE/EtOAc=10/1 to 3/1) to afford A13(300 mg, 70.5%) as an oil.

¹H NMR (400 MHz, CDCl3) δ 3.67 (s, 3H), 2.37-2.31 (m, 2H), 1.96-1.74 (m,9H), 1.72-1.57 (m, 8H), 1.48-1.37 (m, 9H), 1.30-1.22 (m, 7H), 1.17-1.01(m, 6H), 0.61 (s, 3H)

Synthesis of A14 & A15

To a solution of A13 (300 mg, 0.691 mmol) in toluene (5 mL) was addedtrimethylaluminium (1.03 mL, 2 M in toluene) at 25° C. After stirring at65° C. for 16 h, the mixture was poured into water (30 mL) and extractedwith EtOAc (2×20 mL). The combined organic solution was washed withbrine (30 mL), dried over Na₂SO₄, filtered and concentrated. The residuewas purified by HPLC separation (column: YMC-Actus Triart C18 100*30mm*5 um, gradient: 65-95% B, Condition: (water (0.05% HCl)-ACN), flowrate: 25 mL/min) to give A14 (25 mg, 9%) and A16 (30 mg) as solids.

A16 (30 mg) was purified was purified by HPLC separation (column:YMC-Actus Triart C18 100*30 mm*5 um, gradient: 70-100% B, Condition:(water (0.05% HCl)-ACN), flow rate: 25 mL/min) to give A16 (16 mg,53.5%) as a solid.

A14: ¹H NMR (400 MHz, CDCl3) δ 4.19-4.13 (m, 1H), 3.57 (s, 1H),2.74-2.71 (m, 1H), 2.39 (s, 2H), 1.94-1.79 (m, 6H), 1.76-1.57 (m, 9H),1.51-1.38 (m, 6H), 1.34-1.30 (m, 1H), 1.29-1.24 (m, 5H), 1.22-1.17 (m,3H), 1.13-1.00 (m, 5H), 0.70 (s, 3H); LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₆H₄₄NO₂ [M+H]⁺ 402, found 402.

A15: ¹H NMR (400 MHz, CDCl3) δ 4.23-4.18 (m, 1H), 3.64-3.60 (m, 1H),2.62-2.57 (m, 1H), 2.40-2.27 (m, 2H), 1.94-1.71 (m, 8H), 1.69-1.59 (m,5H), 1.47-1.31 (m, 7H), 1.29-1.22 (m, 5H), 1.22-1.16 (m, 4H), 1.14-0.96(m, 6H), 0.70 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₆H₄₄NO₂ [M+H]⁺ 402, found 402.

Example 8: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-2-phenylacetamide(A16)

To a suspension of 2-phenylacetic acid (800 mg, 5.88 mmol) and HATU(2.79 g, 7.35 mmol) in DCM (10 mL) under nitrogen at 25° C. was addedEt₃N (2.47 g, 24.5 mmol). After stirring at 25° C. for 30 mins, asolution of A5 (1.5 g, 4.90 mmol) was added. After stirring at 25° C.for 18 h, the mixture was quenched by water (10 mL) and extracted withDCM (2×10 mL). The combined organic solution was washed with brine (2×5mL), dried over Na₂SO₄, filtered and concentrate in vacuum to give A16(1.41 g). The product (150 mg, 0.354 mmol) was purified by HPLC (Column:YMC-Actus Triart C18 100*30 mm*5 um; Condition: water (0.05% HCl)-ACN;Begin B: 70; End B: 95; Gradient Time(min): 8; 100% B Hold Time(min): 1;FlowRate(ml/min): 25; Injections: 6) to afford A16 (36 mg, 24.1%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ 7.38-7.30 (m, 3H), 7.29-7.23 (m, 2H), 5.20 (s,1H), 3.56 (s, 2H), 3.28-3.22 (m, 1H), 3.21-3.12 (m, 1H), 1.87-1.77 (m,3H), 1.76-1.59 (m, 2H), 1.56-1.47 (m, 1H), 1.46-1.31 (m, 9H), 1.30-1.18(m, 7H), 1.06-0.94 (m, 6H), 0.58 (m, 3H); LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₂NO₂ [M+H]⁺ 424, found 424.

Examples 9 & 10: Synthesis of(R)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-4-phenylpyrrolidin-2-one(A18) &(S)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-4-phenylpyrrolidin-2-one(A19)

Synthesis of A17

To a solution of A5 (400 mg, 1.30 mmol) in toluene (10 mL) was addedbenzenepropanoic acid, β-formyl-, methyl ester (499 mg, 2.60 mmol).After stirring at 115° C. for 3 h, the reaction was cooled to 25° C. andMeOH (20 ml) and borane sodium hydride (123 mg, 3.25 mmol) were added.After stirring at 25° C. for 1 h, the mixture was poured into ice-water(50 mL) and extracted with EtOAc (2×30 mL). The combined organicsolution was washed with brine (50 mL), dried over Na₂SO₄, filtered andconcentrated in vacuum. The residue was purified by combi flash (0-20%of EtOAc in PE) to give A17 (190 mg, 33%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 7.55-7.41 (m, 5H), 6.44-6.42 (m, 1H),4.38-4.29 (m, 2H), 3.72-3.62 (m, 1H), 3.41-3.31 (m, 1H), 1.88-1.61 (m,9H), 1.49-1.35 (m, 8H), 1.28 (s, 3H), 1.24 (s, 3H), 1.19-0.99 (m, 8H).

Synthesis of A18 & A19

A solution of A17 (190 mg, 0.4244 mmol) in MeOH (20 mL) was added driedPd/C (50 mg) and hydrogenated under H₂ (15 psi) at 20° C. After stirringfor 16 h, the reaction was filtered through a pad of celite andconcentrated to give an oil (100 mg, 53%). The oil was purified by SFC(Column: YMC CHIRAL Amylose-C (250 mm*30 mm, 10 um, Condition: 0.1%NH₃H₂O ETOH, Begin B: 55%, End B: 55%) to give A18 (Peak 1, 21 mg, 18%)and A19 (Peak 2, 20 mg, 17%) both as solids.

A18: ¹H NMR (400 MHz, CDCl₃) δ 7.41-7.29 (m, 3H), 7.25-7.20 (m, 2H),3.76-3.73 (m, 1H), 3.52-3.48 (m, 1H), 3.45-3.41 (m, 1H), 3.40-3.36 (m,1H), 3.28-3.22 (m, 1H), 2.84-2.75 (m, 1H), 2.61-2.52 (m, 1H), 1.91-1.59(m, 10H), 1.51-1.29 (m, 10H), 1.26 (s, 3H), 1.25-0.99 (m, 5H), 0.72 (s,3H); LC-ELSD/MS purity 99%, MS calcd. for C₃₀H₄₄NO₂[M+H]⁺ 450, found450.

A19: ¹H NMR (400 MHz, CDCl₃) δ 7.41-7.29 (m, 3H), 7.25-7.20 (m, 2H),3.76-3.73 (m, 1H), 3.52-3.48 (m, 1H), 3.45-3.36 (m, 2H), 3.28-3.22 (m,1H), 2.84-2.75 (m, 1H), 2.61-2.52 (m, 1H), 1.91-1.59 (m, 10H), 1.51-1.29(m, 10H), 1.26 (s, 3H), 1.25-0.99 (m, 5H), 0.72 (s, 3H); LC-ELSD/MSpurity 99%, MS calcd. for C₃₀H₄₄NO₂[M+H]⁺ 450, found 450.

Examples 11 & 12: Synthesis of(S)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-3-phenylpyrrolidin-2-one(A20) &(R)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-3-phenylpyrrolidin-2-one(A21)

A solution of A16 (500 mg, 1.18 mmol) in THF (5 mL) was added to a cold(−78° C.) solution of lithium di-isopropylamide prepared from theaddition of n-butyl-lithium in hexane (4.6 mL, 2.5 M, 11.5 mmol) todi-isopropylamine (2 mL, 0.72 g/mL, 14.2 mmol) in THF (5 mL) at −78° C.After stirring at −78° C. for 1 h, 1-bromo-2-chloroethane (507 mg, 3.54mmol) was added to the reaction mixture. The reaction was warm to 20° C.and stirred for 16 h. After quenching with water (50 mL), the reactionwas extracted with EtOAc (2×50 mL). The combined organic solution waswashed with brine (100 mL), dried over Na₂SO₄, filtered, concentratedand purified by combi flash (0-30% of EtOAc in PE) to give desiredproduct, which was further purified by SFC (Column: YMC CHIRAL Amylose-C(250 mm*30 mm, 10 um, Condition: 0.1% NH₃H₂O ETOH, Begin B: 50%, End B:50%) to give A20 (Peak 1, 18 mg, 3%) and A21 (Peak 2, 54 mg, 10%) bothas solids.

A20: ¹H NMR (400 MHz, CDCl₃) δ 7.35-7.28 (m, 2H), 7.24-7.19 (m, 3H),3.69-3.61 (m, 1H), 3.49-3.35 (m, 3H), 3.33-3.22 (m, 1H), 2.55-2.45 (m,2H), 2.17-2.09 (m, 1H), 1.92-1.61 (m, 11H), 1.51-1.31 (m, 7H), 1.27 (s,3H), 1.21-0.99 (m, 6H), 0.73 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₃₀H₄₄NO₂[M+H]⁺ 450, found 450.

A21: ¹H NMR (400 MHz, CDCl₃) δ 7.35-7.28 (m, 3H), 7.24-7.19 (m, 2H),3.69-3.61 (m, 1H), 3.49-3.41 (m, 2H), 3.40-3.35 (m, 1H), 3.33-3.22 (m,1H), 2.55-2.45 (m, 2H), 2.17-2.09 (m, 1H), 1.92-1.61 (m, 11H), 1.51-1.31(m, 7H), 1.27 (s, 3H), 1.21-0.99 (m, 6H), 0.71 (s, 3H); LC-ELSD/MSpurity 99%, MS ESI calcd. for C₃₀H₄₄NO₂[M+H]⁺ 450, found 450.

Examples 13 & 14: Synthesis of(R)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-6-phenylpiperidin-2-one(A22) &(S)-1-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)-6-phenylpiperidin-2-one(A23)

To a stirred solution of A5 (300 mg, 0.9819 mmol) in methanol (40 mL)was added methyl 5-oxo-5-phenylpentanoate (241 mg, 1.17 mmol) andNaCNBH₃, (154 mg, 2.45 mmol). The mixture was brought to pH 6 with HOAc(1 mL). After stirring at 70° C. for 48 h, the reaction mixture wasextracted with ethyl acetate (2×80 mL). The combined organic solutionwas washed with brine (100 mL), dried over Na₂SO₄, filtered andconcentrated under vacuum to give a residue, which was triturated inMeCN (20 mL) at 20° C. to give solid (410 mg). The material wasinitially purified by flash column chromatography (ethyl acetate inpetroleum ether, 75%) to give a solid (140 mg, 34%) followed by SFC(column: AD (250 mm*30 mm, 5 um)), gradient: 35-35% B (A=0.1% NH₃/H₂O,B=EtOH), flow rate: 80 mL/min) to give A22 (Peak 1, 50 mg, 36%) and A23(Peak 2, 40 mg, 29%) as solids.

A22: ¹H NMR (400 MHz, CDCl3) δ 7.34 (d, J=4.0 Hz, 4H), 7.30-7.27 (m,1H), 5.32 (s, 1H), 4.70 (s, 1H), 3.40-3.26 (m, 1H), 3.21-3.05 (m, 1H),2.29 (d, J=3.6 Hz, 1H), 2.24-2.15 (m, 2H), 1.88-1.58 (m, 9H), 1.53-1.28(m, 11H), 1.26 (s, 4H), 1.18-0.93 (m, 6H), 0.65 (s, 3H); LC-ELSD/MSpurity 96.6%. MS ESI calcd. for C₃₁H₄₆NO₂ [M+H]⁺ 464, found 464.Analytical SFC 100% de. (condition: Column: ChiralPak AD-3 150×4.6 mmI.D., 3 um; Gradient: 40% of Ethanol (0.05% DEA) in CO₂; Flow rate: 2.5mL/min Column temperature:40° C.).

A23: ¹H NMR (400 MHz, CDCl3) δ 7.34 (d, J=4.4 Hz, 4H), 7.30-7.27 (m,1H), 5.37-5.29 (m, 1H), 4.74-4.66 (m, 1H), 3.40-3.27 (m, 1H), 3.18-3.10(m, 1H), 2.30 (s, 1H), 2.24-2.14 (m, 2H), 1.90-1.58 (m, 9H), 1.53-1.28(m, 11H), 1.26 (s, 4H), 1.19-0.96 (m, 6H), 0.65 (s, 3H); LC-ELSD/MSpurity 98.5%. MS ESI calcd. for C₃₁H₄₆NO₂ [M+H]⁺ 464. Analytical SFC100% de. (condition: Column: ChiralPak AD-3 150×4.6 mm I.D., 3 um;Gradient: 40% of Ethanol (0.05% DEA) in CO₂; Flow rate: 2.5 mL/minColumn temperature:40° C.).

Example 15: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)benzamide(A32)

Synthesis of A25

To a stirred solution of iodotrimethyl-4-sulfane (77.9 g, 382 mmol) andNaH (60%, 15.2 g, 382 mmol) in DMSO (900 mL) was added to a solution ofestrane-3,17-dione (100 g, 364 mmol) in DMSO (300 mL). After stirring at15° C. for 16 h, the reaction was treated with water (1000 mL) andextracted with EtOAc (2×1000 mL). The combined organic solution waswashed with water (2×1000 mL), brine (1000 mL), dried over anhydrousNa₂SO₄, filtered, concentrated in vacuum. The residue was trituratedfrom MeOH (1000 mL) at 65° C. to give filter cake A25a (20 g, 19%) as asolid. The filtrate was concentrated to give A25 (80 g) as an oil. A25(80.0 g) was triturated from MeOH (300 mL) at 65° C. to give filter cake(15 g, mixture) as a solid, and the filtrate to concentrated to give A25(65 g) as an oil.

¹H NMR (400 MHz, CDCl3) δ 2.65-2.55 (m, 2H), 2.48-2.40 (m, 1H),2.28-1.50 (m, 11H), 1.50-1.00 (m, 10H), 0.95-0.90 (m, 1H), 0.88 (s, 3H).

Synthesis of A26

To the fresh prepared ethoxysodium (To a solution of ethanol (50 mL) wasadded Na (8 g, 347 mmol) in five portions at 40° C. under N₂ and stirredat 40° C. for 2 h) in ethanol (50 mL) was added A25 (8 g, 27.7 mmol) at40° C. After stirring at 60° C. for 16 h, the mixture was cooled andpoured into water (150 mL) and extracted with EtOAc (2×300 mL). Thecombined organic solution was washed with brine (150 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜10% of EtOAc in PE) to give A26 (4.9 g, 52.9%, 140 mgfor delivery) as an oil.

¹H NMR (400 MHz, CDCl3) δ 3.53 (q, J=6.8 Hz, 2H), 3.43 (q, J=9.2 Hz,2H), 2.70 (s, 1H), 2.48-2.38 (m, 1H), 2.14-1.61 (m, 9H), 1.54-1.03 (m,16H), 0.86 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd for C₁₉H₂₇O[M—ETOH—H₂O+H]⁺ 271, found 271.

Synthesis of A27

To a mixture of MePPh₃Br (9.78 g, 27.4 mmol) in THF (20 mL) was addedt-BuOK (3.06 g, 27.4 mmol) at 15° C. under N₂. After stirring at 60° C.for 30 min. A27 (4.6 g, 13.7 mmol) in THF (30 mL) was added in portionsbelow 60° C. After stirring at 60 C for 16 h, the reaction mixture wasquenched with H₂O (100 mL) at 15° C. and extracted with EtOAc (2×200mL). The combined organic solution was washed with brine (100 mL), driedover anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜10% of EtOAc in PE) to give A27 (3.7 g,81.3%) as an oil.

¹H NMR (400 MHz, CDCl3) δ 4.66-4.59 (m, 2H), 3.54 (q, J=6.8 Hz, 2H),3.44 (q, J=9.2 Hz, 2H), 2.69 (s, 1H), 2.54-2.43 (m, 1H), 2.30-2.18 (m,1H), 1.77 (s, 7H), 1.52-1.07 (m, 17H), 0.78 (s, 3H).

Synthesis of A28

To a solution of A27 (3.7 g, 11.1 mmol) in THF (40 mL) was added 9-BBNdimer (5.41 g, 22.2 mmol) under N₂. After stirring at 60° C. for 1 h,the mixture was cooled to 15° C. and ethanol (6.38 mL, 111 mmol) andNaOH (22.2 mL, 5 M, 111 mmol) were added. H₂O₂ (11.1 mL, 10 M, 111 mmol)was then added dropwise at 25° C. followed by saturated aqueous Na₂S₂O₃(10 mL). After stirring at 15° C. for another 1 h, the mixture waspoured into water (20 mL) and extracted with EtOAc (2×30 mL). Thecombined organic solution was washed with brine (20 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜35% of EtOAc in PE) to give A28 (2.8 g, 71.9%) as asolid.

¹H NMR (400 MHz, CDCl3) δ 3.76-3.67 (m, 1H), 3.57-3.50 (m, 3H),3.46-3.38 (m, 2H), 2.72-2.67 (m, 1H), 1.88-1.72 (m, 5H), 1.68-1.57 (m,5H), 1.49-1.35 (m, 6H), 1.30-1.01 (m, 12H), 0.65 (s, 3H); LC-ELSD/MSpurity 99%, MS ESI calcd for C₂₂H₃₆O₂[M−H₂O]⁺333, found 333.

Synthesis of A29

To a solution of A28 (2.7 g, 7.70 mmol) in DCM (30 mL) at 15° C. wereadded 1-methyl-1H-imidazole (1.26 g, 15.4 mmol), TEA (1.55 g, 15.4 mmol)and then TsCl (2.93 g, 15.4 mmol). After stirring at 15° C. for 2 h, themixture was washed with water (2×80 mL), brine (80 mL), dried overNa₂SO₄, filtered and concentrated under vacuum to give A29 (4.7 g) as anoil.

¹H NMR (400 MHz, CDCl3) δ 7.79 (d, J=8.0 Hz, 2H), 7.35 (d, J=8.0 Hz,2H), 4.10-4.03 (m, 1H), 3.97-3.90 (m, 1H), 3.71 (s, 1H), 3.57-3.49 (m,2H), 3.46-3.38 (m, 2H), 2.46 (s, 3H), 1.84-1.70 (m, 6H), 1.65-1.55 (m,4H), 1.49-1.33 (m, 6H), 1.23-0.96 (m, 11H), 0.57 (s, 3H).

Synthesis of A30

To a solution of A29 (4.7 g, 9.31 mmol) in DMSO (100 mL) was added NaN₃(1.81 g, 27.9 mmol). After stirring at 70° C. for 16 h, the mixture wascooled and sat.NaHCO₃.aq was added until pH>8. The mixture was extractedwhit EtOAc (2×100 mL) and the combined organic solution was washed withbrine (100 mL), dried over anhydrous Na₂SO₄, filtered and concentratedunder vacuum to give A30 (2.5 g, 72%) as a solid.

¹H NMR (400 MHz, CDCl3) δ 3.53 (q, J=7.2 Hz, 2H), 3.46-3.38 (m, 2H),3.29-3.15 (m, 2H), 2.70 (s, 1H), 1.76 (m, 9H), 1.50-1.32 (m, 7H), 1.20(m, 11H), 0.62 (s, 3H).

Synthesis of A31

A solution of A30 (2.5 g, 6.65 mmol) in THF (25 mL) with Pd/C (0.2 g,water>50%) was hydrogenated under 15 psi of hydrogen. After 3 h, themixture was filtered through a pad of celite and the filtrate wasconcentrated in vacuum to give A31 (1.85 g) as a solid.

¹H NMR (400 MHz, CDCl3) δ 3.53 (q, J=6.8 Hz, 2H), 3.46-3.38 (m, 2H),2.87-2.50 (m, 7H), 1.98-1.56 (m, 9H), 1.38 (m, 7H), 1.20 (t, J=7.2 Hz,9H), 0.60 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd for C₂₂H₄₀NO₂[M+H]⁺ 350, found 350.

Synthesis of A32

To a solution of A31 (300 mg, 0.858 mmol) in anhydrous DCM (5 mL) wasadded TEA (260 mg, 2.57 mmol) and BzCl (240 mg, 1.71 mmol) at 25° C.under N₂. After stirring for 16 h, the mixture was poured into water (20mL) and extracted with EtOAc (2×30 mL). The combined organic solutionwas washed with brine (30 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by HPLC (Column: YMC-Actus TriartC18 100×30 mm, Sum; Condition; water (0.05% HCl)-ACN; Gradient: from 80%to 96% of B in 7.5 min; Flow rate: 25 mL/min; Injections: 8) to give A32(140 mg, 35.9%) as a solid.

¹H NMR (400 MHz, CDCl3) δ 7.77-7.70 (m, 2H), 7.52-7.40 (m, 3H),6.06-5.91 (m, 1H), 3.53 (d, J=6.8 Hz, 3H), 3.42 (d, J=10.8 Hz, 3H),1.95-1.74 (m, 5H), 1.69-1.55 (m, 8H), 1.45-1.34 (m, 6H), 1.27-1.04 (m,10H), 0.72 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd for C₂₉H₄₄NO₃[M+H]⁺ 454, found 454.

Example 16: Synthesis ofN-(((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)methyl)benzamide(A39)

Synthesis of A33

To anhydrous methanol (100 mL) was added Na (6.34 g, 276 mmol) in fiveportions. The mixture was stirred at 25° C. for 2 h. A25 (8.00 g, 27.7mmol) in THF (50 mL) was added to the reaction mixture and stirred at60° C. for 5 h. After the reaction mixture was cooled to 0° C., thereaction mixture was quenched by addition of H₂O (100 mL) and extractedwith EtOAc (3×100 mL). The combined organic solution was washed withsaturated brine (2×100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give A333 (5.352 g, 60%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 3.45-3.35 (m, 5H), 2.63-2.58 (m, 1H),2.49-2.40 (m, 1H), 2.13-2.03 (m, 1H), 1.96-1.56 (m, 8H), 1.56-1.00 (m,13H), 0.86 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₀H₃₁O₂[M−H₂O+H]⁺303, found 303.

Synthesis of A34

To a mixture of MePPh₃Br (11.5 g, 32.4 mmol) in THF (80 mL) was addedt-BuOK (3.62 g, 32.4 mmol) at 15° C. under N₂. After stirring at 60° C.for 30 min. A33 (5.20 g, 16.2 mmol) in THF (20 mL) was added inportions. After stirring at 60° C. for 16 h, the reaction mixture wasquenched with H₂O (50 mL) at 15° C. and extracted with EtOAc (3×50 mL).The combined organic solution was washed with saturated brine (2×50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue wastriturated from CH₃OH: H₂O=1/1 (100 mL) at 15° C. to give A34 (4.50 g,87%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ 4.65-4.58 (m, 2H), 3.45-3.35 (m, 5H), 2.58 (s,1H), 2.50-2.43 (m, 1H), 2.25-2.13 (m, 1H), 1.88-1.56 (m, 8H), 1.56-1.05(m, 13H), 0.77 (s, 3H).

Synthesis of A35

The solution of A34 (5.00 g, 15.6 mmol) in THF (50 mL) was added 9-BBNdimer (7.61 g, 31.2 mmol) under N₂. After stirring at 60° C. under N₂for 1 h, the mixture was cooled to 15° C. and ethanol (8.97 mL, 156mmol) and NaOH (31.2 mL, 5 M, 156 mmol) were added. H₂O₂ (15.6 mL, 10 M,156 mmol) was then added dropwise at 15° C. After stirring at 60° C. for1 h, EtOAc (30 mL) and Na₂S₂O₃ (30 mL) were added at 15° C. Afterstirring for 1 h, the mixture was extracted with EtOAc (3×50 mL). Thecombined organic solution was washed with brine (50 mL) and dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜30% of EtOAc in PE) to give A35 (4.50 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 3.75-3.62 (m, 1H), 3.57-3.52 (m, 1H),3.45-3.35 (m, 5H), 2.58 (brs, 1H), 1.85-1.50 (m, 11H), 1.50-1.00 (m,14H), 0.65 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₁H₃₅O₂[M−H₂O+H]⁺319, found 319.

Synthesis of A36

To a solution of A35 (4.40 g, 13.0 mmol) in DCM (50 mL) at 15° C. wereadded 1-methyl-1H-imidazole (2.13 g, 26.0 mmol), TEA (2.63 g, 26.0 mmol)and then TsCl (4.95 g, 26.0 mmol). After stirring at 15° C. for 2 h, themixture was washed with water (2×100 mL), brine (100 mL), dried overNa₂SO₄, filtered and concentrated under vacuum, which was purified bycolumn (0-20% EtOAc in PE) to give A36 (5.00 g, 78%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 7.80-7.77 (m, 2H), 7.37-7.30 (m, 2H),4.10-4.05 (m, 1H), 3.98-3.92 (m, 1H), 3.42-3.35 (m, 5H), 2.56 (s, 1H),2.46 (s, 3H), 1.83-1.50 (m, 10H), 1.50-0.92 (m, 14H), 0.56 (s, 3H).

Synthesis of A37

To a solution of A36 (5.00 g, 10.1 mmol) in DMSO (100 mL) was added NaN₃(1.96 g, 30.3 mmol). After stirring at 70° C. for 16 h, the mixture wascooled and aqueous 10% NaHCO₃.aq (200 mL) was added until pH>8. Themixture was extracted with EtOAc (2×100 mL) and the combined organicsolution was washed with brine 1 (300 mL), dried over anhydrous Na₂SO₄,filtered and concentrated under vacuum to give A37 (3.50 g, 96%) as anoil.

¹H NMR (400 MHz, CDCl₃) δ 3.45-3.35 (m, 5H), 3.28-3.13 (m, 2H),2.60-2.53 (m, 2H), 1.98-1.50 (m, 12H), 1.50-0.95 (m, 11H), 0.63 (s, 3H)

Synthesis of A38

A solution of A37 (1 g, 2.76 mmol) in THF (10 mL) with Pd/C (0.2 g,water>50%) was hydrogenated at 15 psi. After 3 h, the mixture wasfiltered through a pad of celite and the filtrate was concentrated invacuum to give A38 (1.08 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 8.25 (s, 3H), 3.44-3.34 (m, 5H), 3.07 (s, 1H),2.87-2.61 (m, 2H), 2.07 (s, 1H), 1.65-1.85 (m, 7H), 1.32-1.51 (m, 7H),0.98-1.29 (m, 8H), 0.64 (s, 3H).

Synthesis of A39

To a solution of A38 (300 mg, 0.894 mmol) in DCM (5 mL) was added Et₃N(271 mg, 2.68 mmol) and BzCl (250 mg, 1.78 mmol). After stirring at 20°C. for 16 h, the mixture was quenched with H₂O (5 mL) and extracted withDCM (2×2 mL). The combined organic solution was filtered, concentrated(0.53 g) and purified by prep.HPLC (Column: YMC-Actus Triart C18 100*30mm*5 um; Condition: water (0.05% HCl)-ACN; Begin B:75; End B:93;Gradient Time(min):7; 100% B Hold Time(min):1; FlowRate(ml/min):25;Injections:7) to afford A39 (221 mg, 56%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 7.77-7.70 (m, 2H), 7.54-7.38 (m, 3H),6.04-5.95 (m, 1H), 3.60-3.51 (m, 1H), 3.31-3.45 (m, 6H), 2.60 (s, 1H),1.73-1.95 (m, 5H), 1.6-1.72 (m, 4H), 0.98-1.51 (m, 15H), 0.72 (s, 3H);LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₂NO₂ [M+H]⁺ 440, found440.

Examples 17 & 18: Synthesis ofN—((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)benzenesulfonamide(B4) &N—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)benzenesulfonamide(B5)

Synthesis of B2

To a solution of KOH (2.61 g, 46.7 mmol) in EtOH (50 mL) was added19-Norpregnan-20-one, 3-hydroxy-3-methyl-, (3α,5β)-(B1) (5 g, 15.6 mmol)and hydroxylamine.HCl (2.16 g, 31.2 mmol) at 15° C. After stirring for16 h at 15° C., the reaction was diluted with water (100 mL) andextracted with EtOAc (3×50 mL). The combined organic solution was washedwith brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated under vacuum to give B2 (51 g) as an oil.

Synthesis of B3

To a solution of B2 (4 g, 11.9 mmol) in THF (200 mL) was added LiAlH₄(4.52 g, 119 mmol) at 0° C. After stirring for 16 h at 70° C. thereaction was cooled to 0° C. and H₂O (10 mL and then NaOH (10%, 10 mL)were added. After stirring for 0.5 h at 15° C., the mixture was filteredand the residue was washed with anhydrous THF (2×200 mL). The combinedorganic solution was concentrated in vacuum to give B3 (4.2 g) as anoil.

Synthesis of B4 & B5

To a solution of B3 (1 g, 3.12 mmol) and TEA (630 mg, 6.24 mmol),2,6-dimethylpyridine (667 mg, 6.24 mmol) in DCM (10 mL) was addedbenzenesulfonyl chloride (577 mg, 3.27 mmol) at 0° C. After stirring atrt for 16 h, the mixture was poured in to water (20 mL) and extractedwith ethyl acetate (2×20 mL). The combined organic solution was washedwith brine (10 mL), dried over Na₂SO₄, filtered, concentrated in vacuum,purified by HPLC ((column: YMC-Actus Triart C18 100*30 mm*5 um),gradient: 75-96% B (water (0.05% HCl)-ACN), flow rate: 25 mL/min) andthen by SFC (column: YMC CHIRAL Amylose-C (250 mm*30 mm, 10 um,gradient: 40-40% B (0.1% NH3H2O IPA), flow rate: 70 mL/min) to afford B4(100 mg) and B5 (113 mg, 13%) as solids. B4 (100 mg, 0.2175 mmol) wastriturated from H₂O (10 mL) at 65° C. to give B4 (78 mg, 78%) as asolid.

B4: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.90-7.80 (m, 2H), 7.65-7.40 (m, 3H),4.20-4.10 (m, 1H), 3.35-3.30 (m, 1H), 1.85-1.65 (m, 5H), 1.65-1.50 (m,2H), 1.50-1.25 (m, 7H), 1.25-0.85 (m, 9H), 0.61 (s, 3H); LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₇H₄₁NO₃SNa [M+Na]⁺482, found 482.

B5: ¹H NMR δ_(H) 7.90-7.80 (m, 2H), 7.65-7.40 (m, 3H), 4.20-4.10 (m,1H), 3.35-3.30 (m, 1H), 2.15-1.95 (m, 1H), 1.80-1.70 (m, 3H), 1.70-1.50(m, 2H), 1.50-1.20 (m, 15H), 1.20-0.75 (m, 10H), 0.61 (s, 3H);LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₁NO₃SNa [M+Na]⁺482, found482.

Examples 19 & 20: Synthesis ofN—((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-N-methylbenzamide(B7) &N-((1R)-1-((3R,5R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-N-methylbenzamide(B8)

Synthesis of B6

A solution of B1 (500 mg, 1.6 mmol) in MeNH₂ (7.8 mL, 2M in EtOH, 15.6mmol) was stirred at 25° C. for 10 h. To the reaction mixture was thenadded NaBH₄ (295 mg, 7.8 mmol) at 25° C. The mixture was stirred at 25°C. for 0.5 h, then H₂O (20 mL) was added to the reaction mixture andextracted with EtOAc (3×20 mL). The combined organic solution was washedwith saturated brine (2×20 mL), dried over anhydrous Na₂SO₄, filteredand concentrated to give B6 (700 mg) as a solid.

Synthesis of B7 & B8

To a solution of B6 (700 mg, 2.1 mmol) in pyridine (10 mL) was addedbenzoyl chloride (321 mg, 2.3 mmol). After stirring at 20° C. for 4 h,the reaction mixture was poured into water (20 mL) and extracted withEtOAc (2×20 mL). The combined organic solution was washed with brine(2×20 mL), dried over anhydrous Na₂SO₄, filtered, concentrated, purifiedby HPLC ((column: YMC-Actus Triart C18 100*30 mm*5 um), gradient: 70-99%B (water (0.05% HCl)-ACN), flow rate: 25 mL/min) and purified by SFC(column: DAICEL CHIRALPAK AS (250 mm*30 mm, 10 um, gradient: 30-30% B(0.1% NH₃H₂O ETOH), flow rate: 65 mL/min) to afford B7 (223 mg, 24%) andB8 (78 mg, 9%) as solids.

¹H NMR (400 MHz, CDCl3) δ_(H) 7.40-7.27 (m, 5H), 4.98-4.80 (m, 0.4H),3.75-3.60 (m, 0.6H), 2.93 (s, 1.5H), 2.74 (s, 1.5H), 1.95-1.65 (m, 7H),1.65-1.26 (m, 18H), 1.26-0.85 (m, 6H), 0.83 (s, 1.5H), 0.29 (s, 1.5H);LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₄NO₂ [M+H]⁺ 438, found438.

¹H NMR δ_(H) 7.55-7.27 (m, 5H), 4.98-4.80 (m, 0.5H), 3.75-3.60 (m,0.5H), 2.93 (s, 1.3H), 2.74 (s, 1.8H), 1.95-1.65 (m, 9H), 1.65-1.26 (m,15H), 1.26-0.95 (m, 7H), 0.95-0.82 (m, 2H), 0.81 (s, 1.8H), 0.26 (s,1.2H); LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₄NO₂ [M+H]⁺ 438,found 438.

Examples 21 & 22: Synthesis ofN—((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-N-methylbenzenesulfonamide(B9) &N—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-N-methylbenzenesulfonamide(B10)

To a solution of B6 (700 mg, 2.2 mmol) and TEA (442 mg, 4.4 mmol),2,6-dimethylpyridine (468 mg, 4.4 mmol) in DCM (10 mL) was addedbenzenesulfonyl chloride (404 mg, 2.3 mmol) at 0° C. After stirring at25° C. for 12 h, the mixture was poured in to water (100 mL) andextracted with ethyl acetate (2×50 mL). The combined organic solutionwas washed with brine (100 mL), dried over Na₂SO4, filtered andconcentrated in vacuum, purified by HPLC ((column: YMC-Actus Triart C18100*30 mm*5 um), gradient: 70-99% B (water (0.05% HCl)-ACN), flow rate:25 mL/min) and purified by SFC (column: DAICEL CHIRALPAK AS (250 mm*30mm, 10 um, gradient: 30-30% B (0.1% NH₃H₂O ETOH), flow rate: 65 mL/min)to afford B10 (195 mg, 32.7%) and B9 (72 mg, 12.0%) as solids.

B9: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.74 (d, J=7.2 Hz, 2H), 7.50-7.39 (m,3H), 3.89 (dd, J=6.8, 10.8 Hz, 1H), 2.60 (s, 3H), 1.79-1.67 (m, 4H),1.61-1.48 (m, 4H), 1.46 (s, 7H), 1.29-1.18 (m, 7H), 1.06-0.95 (m, 5H),0.74 (d, J=6.4 Hz, 3H), 0.67 (s, 3H); LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₄NO₃S [M+H]⁺ 474, found 474.

B10: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.77-7.73 (m, 2H), 7.52-7.41 (m, 3H),4.01-3.92 (m, 1H), 2.57 (s, 3H), 2.11-2.07 (m, 1H), 1.82-1.71 (m, 3H),1.59-1.51 (m, 4H), 1.43-1.29 (m, 7H), 1.26-1.13 (m, 7H), 1.04-0.97 (m,5H), 0.82 (s, 3H), 0.54 (d, J=6.4 Hz, 3H); LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₄NO₃S [M+H]⁺ 474, found 474.

Examples 23 & 24: Synthesis ofN—((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)benzamide(B11) &N—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)benzamide(B12)

To a solution of B3 (875 mg, 2.7 mmol) in DCM (10 mL) was added benzoylchloride (574 mg, 4.1 mmol) and TEA (690 mg, 6.8 mmol). After stirringat 15° C. for 16 h, the reaction mixture was poured into water (40 mL)and extracted with EtOAc (2×40 mL). The combined organic solution waswashed with water (2×40 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% EtOAc inPE) to give a mixture of diastereomers (800 mg, 69.5%). Thediastereomers were separated by SFC (Column: Chiralpak AD-3 150iÁ4.6 mmI.D., 3 um, Mobile solution: A: CO₂ B:ethanol (0.05% DEA),Gradient: from5% to 40% of B in 5 min and hold 40% for 2.5 min, then 5% of B for 2.5min, Flow rate: 2.5 mL/min Column temp.: 35° C., ABPR: 1500 psi) toafford B12 (232 mg, 29.1%) and B11 (279 mg, 35%) as solids.

B12: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.77-7.72 (m, 2H), 7.54-7.34 (d,J=7.5 Hz, 3H), 5.89 (d, J=9.3 Hz, 1H), 4.20 (d, J=5.5 Hz, 1H), 1.95-1.75(d, J=9.0 Hz, 5H), 1.68-1.58 (m, 3H), 1.48-1.33 (m, 8H), 1.25 (s, 6H),1.23-1.20 (m, 1H), 1.17 (d, J=6.3 Hz, 3H), 1.14-0.95 (m, 5H), 0.74 (s,3H); LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₂NO₂ [M+H]+ 424,found 424.

B11: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.76-7.70 (m, 2H), 7.43 (d, J=7.5 Hz,3H), 5.88 (d, J=9.0 Hz, 1H), 4.22 (d, J=6.3 Hz, 1H), 1.98-1.75 (s, 5H),1.68-1.59 (m, 3H), 1.54-1.33 (m, 8H), 1.31-1.02 (m, 15H), 0.77 (s, 3H);LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₂NO₂ [M+H]+ 424, found424.

Examples 25 & 26: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-3,13-dimethyl-17-((R)-1-(phenylamino)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(B14) &(3R,5R,8R,9R,10S,13S,14S,17S)-3,13-dimethyl-17-((S)-1-(phenylamino)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(B15)

Synthesis of B13

To a solution of B1 (700 mg, 2.2 mmol) and aniline (1.01 g, 10.9 mmol)in toluene (10 mL) were added molecular sieve 4 A (2.8 g) and then TsOH(113 mg, 0.6 mmol) at 15° C. After stirring the suspension at 120° C.for 3 h, the mixture was concentrated under vacuum to give B13 (4 g),which was used in the next step without purification.

Synthesis of B14 & B15

To a solution of B13 with molecular sieves (4.0 g) in THF (40 mL) at 20°C. was added NaBH₄ (382 mg, 10.1 mmol) and then MeOH (10 mL) dropwise.After stirring at 20° C. for 1 h, the mixture was filtered, and thefiltrate was quenched with saturated NH₄Cl aqueous (50 mL). The mixturewas extracted with DCM (2×50 mL). The combined organic solution waswashed with brine (100 mL), dried over Na₂ SO₄, filtered andconcentrated and purified by flash column (0˜30% EtOAc in PE) to give amixture of diastereomers (400 mg) that was separated by SFC (Column:Chiralcel OJ-3 150iÁ4.6 mm I.D., 3 um Mobile solution: A: CO₂ B:ethanol(0.05% DEA) Gradient: from 5% to 40% of B in 5 min and hold 40% for 2.5min, then 5% of B for 2.5 min Flow rate: 2.5 mL/min Column temp.: 35iæABPR: 1500 psi) to afford B14 (80 mg, 20%) and B15 (30 mg, 7.51%) assolids.

B14: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.15 (br d, J=1.0 Hz, 2H), 6.62 (s,1H), 6.53 (d, J=7.8 Hz, 2H), 3.39 (br dd, J=6.0, 9.8 Hz, 1H), 2.13-2.04(m, 1H), 1.80 (s, 4H), 1.68-1.63 (m, 4H), 1.26 (s, 15H), 1.08 (d, J=6.0Hz, 7H), 1.01-0.93 (m, 2H), 0.66 (s, 3H); LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₇H₄₂NO [M+H]⁺ 396, found 396.

B15: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.14 (s, 2H), 6.63 (s, 1H), 6.55 (brd, J=8.0 Hz, 2H), 3.37 (br s, 1H), 1.95 (br s, 2H), 1.80 (br s, 3H),1.69-1.60 (m, 3H), 1.41 (br s, 8H), 1.27 (s, 7H), 1.18 (br d, J=5.8 Hz,9H), 0.74 (s, 4H); LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₂NO[M+H]⁺ 396, found 396.

Examples 27 & 28: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-17-((R)-1-(benzylamino)ethyl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(B16) &(3R,5R,8R,9R,10S,13S,14S,17S)-17-((S)-1-(benzylamino)ethyl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(B17)

To a solution B1 (8.00 g, 25.1 mmol) in MeOH (100 mL) was added1-phenylmethanamine (16.0 g, 150 mmol) and the pH of the solution wasadjusted to pH 6 with acetic acid (10 mL) and THF (100 mL) at 25° C.under N₂. After stirring at 25° C. for 10 min, NaBH₃CN (1.48 g, 25.1mmol) was added. After 1 h at 65° C., the reaction mixture was cooled,diluted with water (200 mL) and extracted with EtOAc (3×200 mL). Thecombined organic solution was washed with saturated brine (2×200 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜10% of DCM in CH₃OH) to give B16 (5.00 g)and B17 (5.00 g) as solids.

B16: ¹H NMR (400 MHz, CDCl₃) δ=7.34-7.21 (m, 5H), 3.93-3.57 (m, 2H),2.70-2.58 (m, 1H), 2.06-1.98 (m, 1H), 1.95-1.56 (m, 8H), 1.70-1.18 (m,15H), 1.18-0.95 (m, 8H), 0.62 (s, 3H)

B17: ¹H NMR (400 MHz, CDCl₃) δ=7.34-7.27 (m, 5H), 3.93-3.57 (m, 2H),2.63-2.52 (m, 1H), 1.95-1.75 (m, 5H), 1.70-1.30 (m, 11H), 1.28-0.95 (m,16H), 0.65 (s, 3H)

Example 29: Synthesis ofN—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)nicotinamide(B19)

Synthesis of B18

To a solution of B16 (5.00 g, 12.2 mmol) in EtOH (50 mL) was addedPd—C(dry, 500 mg) under N₂. The suspension was degassed under vacuum andpurged with H₂ for three times. The mixture was stirred under H₂ (15psi) at 25° C. for 16 h to give a suspension. The reaction mixture wasfiltered through a pad of Celite and washed with THF (3×10 mL). Thefiltrate was concentrated to give B18 (3 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ=2.90-2.80 (m, 1H), 2.00-1.56 (m, 12H),1.56-1.12 (m, 18H), 1.00-0.98 (m, 3H), 0.73 (s, 3H).

Synthesis of B19

To a solution of B18 (200 mg, 0.625 mmol) in DMF (3 mL) was added HATU(475 mg, 1.25 mmol) and DIPEA (403 mg, 3.12 mmol). After stirring for 15mins at 25° C., pyridine-3-carboxylic acid (153 mg, 1.25 mmol) wasadded. After stirring for 16 h at 25° C., the reaction mixture wasdiluted with EtOAc (10 mL), washed with water (10 mL), 3% of LiClaqueous (10 mL), water (10 mL) and brine (10 mL), dried over sodiumsulfate, filtered and concentrated. The residue was purified by HPLC(Column Agela DuraShell 150 mm_25 mm_5 um; Condition water (0.04%NH3H2O+10 mM NH4HCO3)-ACN Begin B 48 End B 78 Gradient Time(min) 8.5;100% B Hold Time(min) 2 FlowRate (ml/min) 30; Injections 10) to give B19(48 mg, 18%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ=8.94 (d, J=1.8 Hz, 1H), 8.75-8.70 (m, 1H),8.11 (td, J=2.0, 7.8 Hz, 1H), 7.40 (dd, J=5.0, 8.3 Hz, 1H), 5.90 (br d,J=9.3 Hz, 1H), 4.41-4.04 (m, 1H), 1.90-1.58 (m, 8H), 1.52-1.30 (m, 10H),1.30-0.95 (m, 13H), 0.74 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₇H₄₁N₂O₂ [M+H]⁺ 425, found 425.

Example 30: Synthesis ofN—((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)nicotinamide(B21)

Synthesis of B20

To a solution of B17 (2.00 g, 4.88 mmol) in EtOH (20 mL) was addedPd—C(dry, 200 mg) under N₂. The suspension was degassed under vacuum andpurged with H₂ for three times. The mixture was stirred under H₂ (15psi) at 25° C. for 16 h to give a suspension. The reaction mixture wasfiltered through a pad of Celite and washed with THF (3×10 mL). Thefiltrate was concentrated to give B20 (1.7 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ=2.83-2.72 (m, 1H), 2.00-1.75 (m, 7H),1.56-1.25 (m, 18H), 1.25-0.95 (m, 8H), 0.65 (s, 3H).

Synthesis of B21

To a solution of pyridine-3-carboxylic acid (153 mg, 1.25 mmol) in DMF(5 mL) was added HATU (356 mg, 0.937 mmol) and DIPEA (403 mg, 3.12mmol). After stirring for 15 mins at 25° C., B20 (200 mg, 0.625 mmol)was added. After stirring for 16 h at 25° C., the reaction mixture wasdiluted with EtOAc (10 mL), washed with water (10 mL), 3% of LiClaqueous (10 mL), water (10 mL) and brine (10 mL), dried over sodiumsulfate, filtered and concentrated. The residue was purified byprep-HPLC (Column Boston Prime C18 150*30 mm Sum; Condition water (0.05%ammonia hydroxide v/v)-ACN Begin B 60; End B 90 Gradient Time(min) 8100% B Hold Time(min) 0.1 FlowRate(ml/min) 25; Injections 8) to give B21(101 mg, 38%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ=8.91 (d, J=1.5 Hz, 1H), 8.71 (dd, J=1.6, 4.9Hz, 1H), 8.08 (d, J=7.8 Hz, 1H), 7.43-7.34 (m, 1H), 5.91 (br d, J=8.8Hz, 1H), 4.33-4.20 (m, 1H), 2.00-1.75 (m, 5H), 1.70-1.56 (m, 12H),1.56-1.00 (m, 14H), 0.77 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₇H₄₁N₂O₂ [M+H]⁺ 425, found 425.

Examples 31 & 32:Synthesis of5-cyano-N—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)picolinamide(B25) &5-cyano-N—((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)picolinamide(B26)

Synthesis of B23

To a solution of B22 (1 g, 2.86 mmol) and 1-phenylmethanamine (1.83 g,17.1 mmol) in MeOH (15 mL) at 25° C. adjusted to pH 6 (with acetic acidand anhydrous THF) was added after 30 mins NaBH₃CN (215 mg, 3.43 mmol).After stirring at 80° C. for 16 h, the solution was diluted with NaHCO₃aqueous (20 mL) and extracted with EtOAc (2×30 mL). The combined organicsolution was washed with brine, dried over sodium sulfate, concentratedand purified by column chromatography on silica gel (50-80% of EtOAc inPE) to give desired product (1.09 g) as a solid.

¹H NMR (400 MHz, CDCl3) δ 7.36-7.28 (m, 4H), 7.25-7.20 (m, 1H),3.90-3.86 (m, 1H), 3.63-3.59 (m, 1H), 3.42-3.35 (m, 5H), 2.66-2.53 (m,1H), 2.04-1.69 (m, 6H), 1.66-1.52 (m, 7H), 1.47-1.30 (m, 8H), 1.28-0.96(m, 8H), 0.64-0.62 (m, 3H).

Synthesis of B24

To a solution of B23 (1.09 g, 2.47 mmol) in EtOH (10 mL) was added Pd/C(dry, 100 mg). The mixture was stirred under H₂ (15 psi) at 25° C. for16 h. The reaction mixture was filtered through a pad of Celite andwashed with MeOH (3×10 mL). The filtrate was concentrated to give B24(700 mg) as a solid. The product was purified by flash column (2% ofMeOH in CH₂Cl₂) to give B24 (500 mg, 71%) as an oil.

¹H NMR (400 MHz, CDCl3) δ 3.42-3.36 (m, 5H), 2.88-2.77 (m, 1H),2.01-1.71 (m, 6H), 1.70-1.49 (m, 8H), 1.48-1.16 (m, 8H), 1.14-0.97 (m,8H), 0.72-0.65 (m, 3H).

Synthesis of 25 & 26

To a solution of 5-cyanopicolinic acid (500 mg, 3.37 mmol) in DCM (30mL) and DMF (1 mL) was added oxalyl chloride (431 mg, 3.37 mmol)dropwise at 0° C. After stirring at 10° C. for 18 h, DIPEA (147 mg, 1.14mmol) and B24 (100 mg, 0.286 mmol) were added. After stirring at 25° C.for 48 h, saturated NH₄Cl aqueous (50 mL) was added to the mixture andextracted with ethyl acetate (3×30 mL). The combined organic solutionwas washed with aq. LiCl (3×50 mL), dried over Na₂SO₄ and filteredconcentrated in vacuum. The product was purified by flash column (20%EtOAc in PE) to give a mixture of diastereomers (180 mg) as an oil. Thediastereomers were separated by SFC {Column: DAICEL CHIRALPAK AD (250mm*30 mm, 10 um), Condition: 0.1% NH₃H₂O ETOH, Begin B: 40%, End B: 40%}and lyophilized to afford B25 (20 mg, Peak 1) and B26 (22 mg, Peak 2) assolids.

B25: ¹H NMR (400 MHz, CDCl3) δ 8.83 (d, J=1.2 Hz, 1H), 8.34-8.32 (m,1H), 8.14-8.11 (m, 1H), 7.83 (d, J=9.2 Hz, 1H), 4.15-4.09 (m, 1H),3.41-3.34 (m, 5H), 2.60 (s, 1H), 1.89-1.68 (m, 5H), 1.67-1.49 (m, 8H),1.47-1.29 (m, 8H), 1.25-0.85 (m, 6H), 0.68 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₉H₄₂N₃O₃ [M+H]⁺ 480, found 480. SFC 100% de.

B26: ¹H NMR (400 MHz, CDCl3) δ 8.82-8.80 (m, 1H), 8.33-8.31 (m, 1H),8.13-8.11 (m, 1H), 7.83 (d, J=9.2 Hz, 1H), 4.22-4.13 (m, 1H), 3.42-3.36(m, 5H), 2.61 (s, 1H), 1.85 (m, 5H), 1.96-1.68 (m, 8H), 1.67-1.53 (m,5H), 1.51-1.32 (m, 3H), 1.28-1.01 (m, 6H), 0.75 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₉H₄₂N₃O₃ [M+H]⁺ 480, found 480. SFC 100%de.

Example 33: Synthesis of4-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)amino)benzonitrile(C1)

To a solution of 4-bromobenzonitrile (127 mg, 0.703 mmol) in toluene (5mL) was added (acetyloxy)palladio acetate (10.5 mg, 0.047 mmol), Cs₂CO₃(305 mg, 0.938 mmol) and BANAP (29.2 mg, 0.047 mmol) under N₂. Afterstirring at 25° C. for 20 min, B18 (150 mg, 0.469 mmol) was added andthe mixture. After stirring at 110° C. under N₂ for 6 h, the reactionwas cooled to 25° C. and stirred overnight. The reaction mixture wasfiltered and concentrated. The residue was purified by HPLC (ColumnXtimate C18 150*25 mm*5 um; Condition water (0.225% FA)-ACN Begin B 84;End B 100 Gradient Time(min) 7; 100% B Hold Time(min) 2 FlowRate(ml/min)25; Injections 5) to afford C₁ (80 mg, 41%) as a solid.

¹H NMR (400 MHz, CDCl3) δ=7.39 (d, J=8.8 Hz, 2H), 6.47 (d, J=8.8 Hz,2H), 3.95 (br d, J=8.9 Hz, 1H), 3.51-3.31 (m, 1H), 1.93-1.75 (m, 5H),1.70-1.53 (m, 2H), 1.49-1.23 (m, 15H), 1.15-0.95 (m, 9H), 0.62 (s, 3H);LCMS purity 99%, MS ESI calcd. for C₂₈H₄₁N₂₀ [M+H]⁺ 421, found 421.

Examples 34 to 37: Synthesis of1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(C5) (Example 34),1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(C6) (Example 35),1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(C7) (Example 36), &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(C8) (Example 37)

Synthesis of C2

To a solution of MePPh₃Br (12.2 g, 34.0 mmol) in THF (20 mL) was addedt-BuOK (2.88 g, 25.8 mmol) at 15° C. After stirring for 1 h at 15° C.,B22 (3 g, 8.60 mmol) in THF (20 mL) was added. After stirring at 45° C.for 3 h, the mixture was treated with saturated NH₄Cl (50 mL) andextracted with EtOAc (2×30 mL). The combined organic solution was washedwith brine (100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜40% of EtOAcin PE) to give C₂ (4.5 g, curde) as an oil.

¹H NMR (400 MHz, CDCl₃) δ 4.83 (s, 1H), 4.45 (s, 1H), 3.47-3.31 (m, 5H),2.61 (s, 1H), 2.05-2.02 (m, 1H), 1.91-1.77 (m, 4H), 1.74 (s, 3H),1.68-1.52 (m, 5H), 1.49-1.31 (m, 7H), 1.28-1.04 (m, 7H), 0.59-0.50 (m,3H).

Synthesis of C3

To a solution of C2 (4.5 g, 12.9 mmol) in THF (30 mL) was added BH₃.Me₂S(11.6 mL, 116 mL). After stirring at 15° C. for 1 h, aqueous NaOH (6.16g, 154 mmol in water) was added at 0° C. followed by hydrogen peroxide(15.4 mL, 10M in water, 154 mmol). After stirring at 78° C. for 3 h, theresidue was poured into water (35 mL) and extracted with EtOAc (3×30mL). The combined organic solution was washed with brine (2×20 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue wastriturated from CH₃OH (20 mL) and water (200 mL) to give C3 (4.5 g) asan oil, which was purified by flash column (0˜30% of EtOAc in PE) togive C3 (1.7 g, 38%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 3.77-3.69 (m, 0.6H), 3.62 (dd, J=3.3, 10.5 Hz,0.4H), 3.40-3.36 (m, 6H), 1.94 (d, 12.5 Hz, 1H), 1.87-1.71 (m, 6H),1.67-1.52 (m, 4H), 1.49-1.29 (m, 7H), 1.22-0.99 (m, 10H), 0.94 (d, J=6.8Hz, 2H), 0.66 (s, 3H).

Synthesis of C4

To a solution of C3 (1.3 g, 3.56 mmol) in CH₂Cl₂ (15 mL) at 0° C. wasadded PPh₃ (1.11 g, 4.27 mmol) and NBS (755 mg, 4.27 mmol). Afterstirring at 20° C. for 3 h, the reaction mixture was poured into water(20 mL) and extracted with EtOAc (3×30 mL). The combined organicsolution was washed with brine (2×20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜15% of EtOAc in PE) to give C4 (1.0 g, 59%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ 3.63 (dd, J=3.2, 9.6 Hz, 0.6H), 3.53-3.47 (m,0.4H), 3.43-3.33 (m, 6H), 1.97-1.87 (m, 1H), 1.86-1.78 (m, 3H),1.64-1.51 (m, 4H), 1.64-1.51 (m, 4H), 1.48-1.32 (m, 6H), 1.29-1.19 (m,3H), 1.13-0.95 (m, 8H), 0.67 (s, 3H).

Synthesis of C5, C6, C7 & C8

To a solution of C4 (450 mg, 1.05 mmol) in DMF (10 mL) was added Cs₂CO₃(682 mg, 2.1 mmol) and 1H-pyrazole-3-carbonitrile (195 mg, 2.1 mmol).After stirring at 85° C. for 12 h, the reaction mixture was diluted withEtOAc (50 mL) and washed by water (20 mL), aq. LiCl (50 mL, 3%) andbrine (50 mL), dried over anhydrous Na₂SO₄, filtered and concentratedunder vacuum. The residue was purified by flash column (8% of EtOAc inPE) to give C5 & C8 (130 mg) and C6 & C7 (300 mg) as oils.

The mixture of C5 & C8 (130 mg) was purified by SFC (Column: DAICELCHIRALCEL OJ-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃H₂O ETOH, BeginB: 20%, End B: 20%), then concentrated and lyophilized to give C5 (12mg, Peak 1) and C8 (26 mg, Peak 2), both as solids.

The mixture of C6 & C7 was purified by SFC (Column: DAICEL CHIRALCELOD-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃.H₂O ETOH, Begin B: 30%,End B: 30%), then concentrated and lyophilized C6 (83 mg Peak 1) and C7(97 mg Peak 2), both as solids.

C5: ¹H NMR (400 MHz, CDCl3) δ 7.57 (d, J=2 Hz, 1H), 6.76 (d, J=2 Hz,1H), 4.39-4.35 (m, 1H), 3.94-3.88 (m, 1H), 3.42-3.36 (m, 5H), 2.59 (s,1H), 2.15-1.64 (m, 6H), 1.60-1.52 (m, 8H), 1.49-1.31 (m, 5H), 1.27-0.98(m, 6H), 0.81 (d, J=6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, MSESI calcd. for C₂₇H₄₁N₃O₂Na [M+Na]⁺462, found 462. SFC 98.79% de.

C6: ¹H NMR (400 MHz, CDCl3) δ 7.39 (d, J=2.8 Hz, 1H), 6.65 (d, J=2.4 Hz,1H), 4.29-4.25 (m, 1H), 3.75-3.69 (m, 1H), 3.42-3.33 (m, 5H), 2.60 (s,1H), 2.05-1.71 (m, 6H), 1.65-1.55 (m, 6H), 1.48-1.27 (m, 6H), 1.09 (m,7H), 1.21-0.98 (d, J=6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS purity 99%,MS ESI calcd. for C₂₇H₄₁N₃O₂Na [M+Na]⁺462, found 462. SFC 100% de.

C7: ¹H NMR (400 MHz, CDCl3) δ 7.39 (d, J=2.8 Hz, 1H), 6.65 (d, J=2.4 Hz,1H), 4.53-4.48 (m, 1H), 3.70-3.64 (m, 1H), 3.42-3.36 (m, 5H), 2.61 (s,1H), 2.16-2.05 (m, 1H), 1.89-1.71 (m, 5H), 1.66-1.52 (m, 7H), 1.49-1.31(m, 6H), 1.27-1.01 (m, 6H), 0.79 (s, 3H), 0.67 (d, J=6.4 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₁N₃O₂Na [M+Na]⁺462, found462. SFC 100% de.

C8: ¹H NMR (400 MHz, CDCl3) δ 7.56 (d, J=2 Hz, 1H), 6.77 (d, J=2 Hz,1H), 4.61-4.57 (m, 1H), 3.93-3.87 (m, 1H), 3.43-3.36 (m, 5H), 2.59 (s,1H), 2.23-2.14 (m, 1H), 1.93-1.71 (m, 5H), 1.67-1.52 (m, 8H), 1.49-1.31(m, 5H), 1.28-1.01 (m, 6H), 0.82 (s, 3H), 0.68 (d, J=6.4 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₂N₃O₂ [M+H]⁺ 440, found440. SFC 97% de.

Examples 38 & 39: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-3,13-dimethyl-17-((R)-1-(methyl(phenyl)amino)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(C9) &(3R,5R,8R,9R,10S,13S,14S,17S)-3,13-dimethyl-17-((S)-1-(methyl(phenyl)amino)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(C10)

To a solution of B14/B15 (740 mg, 1.87 mmol) and (HCHO)_(n) (561 mg,18.7 mmol) in DCE (20 mL) at 25° C. was added NaBH(OAc)₃ (470 mg, 7.48mmol). After stirring at 25° C. for 16 h, additional (HCHO)_(n) (561 mg,18.7 mmol) and NaCNBH₃ (620 mg) were added. After stirring overnight,the reaction was poured into water (30 mL) and extracted with EtOAc(3×30 mL). The combined organic solution was washed with saturated brine(2×20 mL), dried over anhydrous Na₂SO₄, filtered and concentrated togive a mixture of C9 & C10 (800 mg) as an oil, which was furtherpurified by prep-HPLC (column: Xbridge 150*30 mm*10 um; Condition water(10 mM NH₄HCO₃)-ACN from 95% to 100% in 7 min; 100% B Hold Time: 1 min;FlowRat: 25 ml/min) to give mixture of C9 & C10 (260 mg, 0.6346 mmol) asan oil. Purification by SFC (Column: DAICEL CHIRALPAK AD-H (250 mm*30mm, 5 um), Condition:0.1% NH₃H₂O ETOH, Begin B:40%, End B:40%,FlowRate(ml/min):50,Injections:70) to afford C9 (76 mg, Peak 1) and C10 (62 mg,Peak 2) as solids.

C9: ¹H NMR (400 MHz, CDCl3) δ 7.26-7.20 (m, 2H), 6.79-6.77 (m, 2H),6.75-6.65 (m, 1H), 3.85-3.77 (m, 1H), 2.65 (s, 3H), 1.81-1.77 (m, 6H),1.75-1.49 (m, 7H), 1.48-1.31 (m, 6H), 1.30-1.24 (m, 3H), 1.23-1.09 (m,4H), 1.08-0.96 (m, 5H), 0.64 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. For C₂₈H₄₄NO [M+H]⁺ 410, found 410. SFC 100% de.

C10: ¹H NMR (400 MHz, CACl3) δ 7.22-7.18 (m, 2H), 6.75-6.72 (m, 2H),6.65-6.61 (m, 1H), 3.85-3.79 (m, 1H), 2.69 (s, 3H), 2.00-1.77 (m, 5H),1.75-1.60 (m, 4H), 1.59-1.50 (m, 7H), 1.49-1.24 (m, 8H), 1.23-1.06 (m,7H), 0.77 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. For C₂₈H₄₄NO[M+H]⁺ 410, found 410. SFC 100% de.

Example 40: Synthesis of2-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1-oxoisoindoline-5-carbonitrile(D3)

Synthesis of D2

To a mixture of 5-cyanophthalide (1 g, 6.28 mmol) in thionyl chloride(20 mL) was added BF₃.Et₂O (100 mg, 0.8849 mmol) followed bybenzyltriethylammonium chloride (858 mg, 3.77 mmol). After stirring at90° C. for 72 h, the reaction mixture was cooled and then concentratedin vacuum. The resulting residue was dissolved in dry CH₂Cl₂ (100 mL),cooled in an ice-EtOH bath for 5 min, and dry MeOH (50 mL) was addeddropwise. After adjusting with DIPEA to pH 8, the mixture wasconcentrated, diluted with EtOAc (300 mL) and filtered. The filtrate wasconcentrated and purified by silica gel chromatography (3% of ethylacetate in PE) to afford D2 (1.30 g, 99%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.06 (d, J=8.0 Hz, 1H), 7.90 (d, J=1.0 Hz,1H), 7.69 (dd, J=1.6, 8.0 Hz, 1H), 5.03 (s, 2H), 3.97 (s, 3H).

Synthesis of D3

A mixture of D2 (392 mg, 1.87 mmol), B18 (300 mg, 0.9388 mmol), andK₂CO₃ (387 mg, 2.81 mmol) in EtOH (15 mL) was stirred at 25° C. for 1 h.After stirring at 95° C. for 72 h, the reaction mixture was diluted withDCM (100 mL), washed with water (100 mL), brine (100 mL), dried overNa₂SO₄, filtered and concentrated under vacuum to give a solid, whichwas purified by prep-HPLC (Column: Xtimate C18 150*25 mm*5 um,Condition: water (0.225% FA)-ACN, Begin B: 82%, End B: 95%, GradientTime(min): 7, 100% B Hold Time(min): 1, FlowRate(ml/min) 25) to give D3(110 mg) as a solid. The solid was triturated in hexane (20 mL) to givea solid (87 mg, 20%).

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.94 (d, J=8.8 Hz, 1H), 7.78-7.73 (m, 2H),4.58-4.47 (m, 1H), 4.44-4.34 (m, 2H), 1.90-1.59 (m, 6H), 1.50-1.27 (m,9H), 1.25-1.20 (m, 8H), 1.19-0.84 (m, 8H), 0.80 (s, 3H); LC-ELSD/MSpurity 99%, MS ESI calcd. for C₃₀H₄₁N₂O₂ [M+H]⁺ 461, found 461.

Example 41: Synthesis of6-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)amino)nicotinonitrile(E3)

Synthesis of E1

To a solution of B22 (5.00 g, 14.3 mmol) and (1R)-1-phenylethan-1-amine(10.3 g, 85.8 mmol) in DCE (50 mL) was added NaCNBH₃ (7.06 g, 114 mmol)at 25° C. After stirring at 50° C. for 16 h, the reaction was quenchedwith water (50 mL) and extracted with DCM (2×50 mL). The combinedorganic solution was washed with brine (100 mL), dried over Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜20% of EtOAc in PE) to give ST-320-046-009_2 (4.5 g, 69%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.38-7.27 (m, 4H), 7.24-7.16 (m, 1H), 3.89(q, J=6.3 Hz, 1H), 3.48-3.28 (m, 5H), 2.80-2.66 (m, 1H), 2.56 (s, 1H),2.23 (br d, J=11.8 Hz, 1H), 1.93-1.55 (m, 9H), 1.40-1.21 (m, 13H),1.17-1.01 (m, 5H), 0.89 (d, J=6.0 Hz, 3H), 0.78 (s, 3H). % de>99 (by 1HNMR), SFC 100% de.

Synthesis of E2

To a solution of E1 (4.50 g, 9.91 mmol) in EtOH (50 mL) was addedPd—C(dry, 450 mg) under N₂. The suspension was degassed under vacuum andpurged with H₂ for three times. After stirring under H₂ (50 psi) at 50°C. for 16 h, the reaction mixture was filtered through a pad of Celiteand washed with THF (3×50 mL). The combined filtrate was concentrated togive E2 (3.0 g, 87%) as a solid. The stereochemistry at C20 wereassigned based on ¹H NMR of C21-Me.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.51-3.31 (m, 5H), 2.93-2.72 (m, 1H),2.01-1.91 (m, 1H), 1.87-1.62 (m, 7H), 1.51-1.03 (m, 19H), 1.00 (d, J=6.0Hz, 3H), 0.72 (s, 3H).

Synthesis of E3

To a solution of 6-chloropyridine-3-carbonitrile (118 mg, 0.858 mmol) intoluene (2 mL) was added Pd(OAc)₂ (9.63 mg, 0.043 mmol), Cs₂CO₃ (279 mg,0.858 mmol) and BINAP (26.7 mg, 0.043 mmol) under N₂. After stirring at25° C. for 20 min, E2 (150 mg, 0.429 mmol) was added. After stirring at110° C. for 32 h, the reaction mixture was filtered and concentrated.The residue was purified by flash column (0˜50% of EtOAc in PE) to givea solid. The solid was purified by HPLC (Column Xtimate C18 150*25 mm*5um; Condition water (0.225% FA)-ACN Begin B 80 End B 100 GradientTime(min) 7; 100% B Hold Time(min) 1 FlowRate(ml/min) 25) to afford E3(24 mg, 15% mmol) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 8.34 (d, J=2.0 Hz, 1H), 7.53 (br d, J=7.3Hz, 1H), 6.29 (d, J=8.8 Hz, 1H), 4.80 (br s, 1H), 3.44-3.22 (m, 5H),2.63 (br s, 1H), 1.92-1.63 (m, 7H), 1.56-1.27 (m, 10H), 1.26-0.89 (m,11H), 0.62 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₂N₃O₂[M+H]⁺ 452, found 452.

Example 42: Synthesis ofN—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-2-fluorobenzamide(F9)

Synthesis of F2

To a stirred solution of trimethylsulfonium iodide (70 g, 343 mmol) inDMSO (200 mL) and THF (100 mL) was added NaH (14 g, 583 mmol) at 0° C.for 2 h under N₂. To the mixture was added a solution ofestrane-3,17-dione, (5β)-(50 g, 182 mmol) in DMSO (200 mL) and THF (100mL) at 0° C. After stirring at 25° C. for 16 h, the reaction mixture waspoured into H₂O (500 mL) and extracted with EtOAc (2×700 mL). Thecombined organic solution was washed with water (2×300 mL), brine (300mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by silica gel chromatography (PE/EtOAc=0-9/1 to 4/1) toafford F2 (37 g) as an oil, which was triturated with MeOH (200 mL) at25° C. to give F2 (27 g, 52%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.63-2.55 (m, 3H), 2.48-2.40 (m, 1H),2.27-2.19 (m, 1H), 2.12-1.76 (m, 7H), 1.71-1.64 (m, 2H), 1.53 (m, 8H),1.18-1.09 (m, 2H), 1.04-0.98 (m, 1H), 0.89-0.87 (m, 3H).

Synthesis of F3

To anhydrous EtOH (200 mL) was added NaH (22.4 g, 933 mmol) at 25° C. inportions. After stirring at 25° C. for 1 h, F2 (27 g, 93.6 mmol) inanhydrous ethanol (100 mL) was added to the fresh prepared ethoxysodiumsolution. After stirring at 75° C. for 16 h, the reaction mixture wascooled, quenched with aqueous NH₄Cl (200 mL) and extracted with EtOAc(2×300 mL). The combined organic solution was washed with brine (200mL), dried over anhydrous Na₂SO₄, filtered, concentrated. The residuewas purified by silica gel chromatography (PE/EtOAc=0 to 9/1 to 4/1) toafford F3 (12.2 g, 39%) and F3a (10.4 g, 33%) as oils.

F3: ¹H NMR (400 MHz, CDCl₃) δ_(H) 3.53 (q, J=6.8 Hz, 2H), 3.42 (q, J=9.2Hz, 2H), 2.72 (s, 1H), 2.43 (dd, J=8.2, 19.2 Hz, 1H), 2.13-2.05 (m, 1H),1.97-1.89 (m, 1H), 1.86-1.74 (m, 5H), 1.66-1.57 (m, 4H), 1.53 (s, 1H),1.52-1.50 (m, 1H), 1.46-1.27 (m, 7H), 1.20 (t, J=6.8 Hz, 4H), 1.12-1.04(m, 1H), 0.86 (s, 3H).

Synthesis of F4

To a mixture of EtPPh₃Br (39.7 g, 107 mmol) in THF (150 mL) was addedt-BuOK (12.0 g, 107 mmol) at 25° C. under N₂. After stirring at 25° C.for 30 min, F3 (12 g, 35.8 mmol) in THF (50 mL) was added. Afterstirring at 75° C. for 16 h, the reaction mixture was diluted with water(200 mL) and extracted with EtOAc (2×300 mL). The combined organicsolution was washed with brine (200 mL), dried over anhydrous Na₂SO₄,filtered and concentrate. The residue was purified by flash column(0˜10% of EtOAc in PE) to give F4 (10.4 g, 84%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-5.07 (m, 1H), 3.53 (q, J=6.8 Hz, 2H),3.43 (q, J=9.2 Hz, 2H), 2.68 (s, 1H), 2.40-2.30 (m, 1H), 2.28-2.13 (m,2H), 1.87-1.69 (m, 4H), 1.67-1.58 (m, 8H), 1.55-1.35 (m, 7H), 1.28-1.23(m, 2H), 1.20 (t, J=7.2 Hz, 4H), 1.17-1.06 (m, 3H), 0.87 (s, 1H).

Synthesis of F5

To a solution of F4 (10.4 g, 30.0 mmol) in THF (200 mL) was added 9-BBNdimer (14.6 g, 60.0 mmol) under N₂. After stirring at 60° C. under N₂for 1 h, the mixture was cooled to 25° C. and ethanol (30 mL, 30.0 mmol)and NaOH (60.0 mL, 5 M, 300 mmol) were added. After turning clear, H₂O₂(30.0 mL, 10 M, 300 mmol) was added dropwise at 25° C. followed bysaturated aqueous Na₂S₂O₃ (100 mL). After stirring at 25° C. for another1 h, the mixture was poured into water (150 mL) and extracted with EtOAc(2×200 mL). The combined organic solution was washed with brine (150mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas triturated from MeOH/H₂O (100 mL/100 mL) at 25° C. to give F5 (11.6g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.52 (q, J=6.8 Hz, 2H), 3.42 (q, J=9.2 Hz,2H), 1.92-1.74 (m, 7H), 1.66-1.55 (m, 8H), 1.45-1.34 (m, 7H), 1.25 (t,J=6.8 Hz, 3H), 1.23-1.19 (m, 6H), 1.16-1.08 (m, 4H), 0.65 (s, 1H).

Synthesis of F6

To a solution of F5 (11.6 g, 31.8 mmol) in DCM (150 mL) was added silicagel (17 g) and PCC (17.0 g, 79.5 mmol) at 25° C. After stirring at 25°C. for 1 h, the mixture was filtered through a pad of celite and washedwith DCM (2×100 mL), filtered and concentrated. The residue was purifiedby flash column (0˜25% of EtOAc in PE) to give F6 (8.5 g, 74%) as anoil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.55-3.49 (m, 2H), 3.46-3.37 (m, 2H),2.79-2.68 (m, 1H), 2.53 (t, J=8.8 Hz, 1H), 2.16-2.11 (m, 1H), 2.10 (s,3H), 2.02-1.96 (m, 1H), 1.85-1.56 (m, 9H), 1.49-1.35 (m, 7H), 1.27-1.18(m, 7H), 1.15-1.01 (m, 3H), 0.60 (s, 1H).

Synthesis of F7

To a solution of F6 (12.8 g, 35.3 mmol) and (1R)-1-phenylethan-1-amine(25.5 g, 211 mmol) in DCE (100 mL) at 25° C. was added NaCNBH₃ (17.7 g,282 mmol). After at 50° C. for 16 h, the reaction was diluted with water(300 mL) and extracted with DCM (2×250 mL). The combined organicsolution was washed with brine (200 mL), dried over Na₂SO₄, filtered andconcentrated. The residue was triturated from MeOH/H₂O (200 mL/200 mL)and purified by flash column (0˜10% of EtOAc in PE) to give F7 (8.8 g,73%) as colorless oil.

¹H NMR (400 MHz, CDCl3) δ 7.36-7.27 (m, 4H), 7.24-7.18 (m, 1H),3.93-3.85 (m, 1H), 3.57-3.49 (m, 2H), 3.48-3.37 (m, 2H), 2.69 (s, 2H),2.26-2.18 (m, 1H), 1.89-1.70 (m, 4H), 1.69-1.55 (m, 5H), 1.45-1.31 (m,6H), 1.28 (d, J=6.4 Hz, 3H), 1.26-1.19 (m, 7H), 1.14-1.01 (m, 5H), 0.89(d, J=6.0 Hz, 3H), 0.78 (s, 3H). % de>99 (by ¹H NMR). SFC 100% de.

Synthesis of F8

To a solution of F7 (8.7 g, 18.6 mmol) in EtOH (100 mL) was addedPd—C(dry, 900 mg) and one drop of NH₃H₂O. After stirring under H₂ (50psi) at 50° C. for 72 h, the reaction mixture was filtered through a padof Celite and washed with EtOH (3×150 mL). The filtrate was concentratedto give F8 (6.7 g, 99%) as oil. The stereochemistry at C20 were assignedbased on ¹H NMR of C21-Me.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.56-3.49 (m, 2H), 3.46-3.37 (m, 2H),2.88-2.79 (m, 1H), 2.00-1.92 (m, 1H), 1.85-1.69 (m, 5H), 1.67-1.54 (m,8H), 1.49-1.28 (m, 8H), 1.20 (t, J=7.2 Hz, 4H), 1.13-1.04 (m, 4H), 1.01(d, J=6.0 Hz, 3H), 0.72 (s, 3H).

Synthesis of F9

To a solution of 2-fluorobenzoic acid (77.0 mg, 0.550 mmol) in pyridine(3 mL) at 25° C. was added EDCI (105 mg, 0.550 mmol). After stirring at25° C. for 30 min. F8 (100 mg, 0.275 mmol) was added. After stirring at50° C. for 16 h, the mixture was diluted with water (20 mL) andextracted with EtOAc (2×30 mL). The combined organic solution was washedwith brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by HPLC (Column: Xtimate C18150×25 mm; Sum; Condition: water (0.225% FA)-ACN; Gradient: from 70% to90% of B in 7 min and hold 100% for 1 min; Flow rate: 25 mL/min) to giveF9 (47 mg, 35%) as solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.16-8.10 (m, 1H), 7.49-7.42 (m, 1H),7.29-7.26 (m, 1H), 7.14-7.07 (m, 1H), 6.68-6.57 (m, 1H), 4.28-4.13 (m,1H), 3.55-3.49 (m, 2H), 3.45-3.36 (m, 2H), 2.70 (s, 1H), 1.89-1.60 (m,7H), 1.54-1.23 (m, 10H), 1.22-1.17 (m, 7H), 1.16-0.88 (m, 6H), 0.73 (s,3H). ¹⁹F NMR (376 MHz, CDCl₃) δ_(F) −113.67. LC-ELSD/MS purity 99%, MSESI calcd. for C₃₀H₄₅FNO₃ [M+H]⁺ 486, found 486.

Example 43: Synthesis of2-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1-oxoisoindoline-5-carbonitrile(F10)

A mixture of D2 (184 mg, 0.88 mmol), F8 (160 mg, 0.44 mmol), and K₂CO₃(182 mg, 1.32 mmol) in EtOH (15 mL) was stirred at 25° C. for 1 h. Afterstirring at 95° C. for 16 h, the reaction mixture was diluted with DCM(100 mL), washed with water (100 mL), brine (100 mL), dried over Na₂SO₄,filtered and concentrated under vacuum to give a solid. The residue waspurified by prep-HPLC (Condition: water (0.225% FA)-ACN, Begin B: 80,End B: 100, Gradient Time (min): 7, 100% B Hold Time (min): 0, FlowRate(ml/min): 25) to give F10 (11 mg, 5%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.94 (d, 1H), 7.81-7.68 (m, 2H), 4.60-4.45(m, 1H), 4.39 (d, J=2.0 Hz, 2H), 3.61-3.46 (m, 2H), 3.44-3.29 (m, 2H),2.71 (s, 1H), 1.88-1.55 (m, 7H), 1.53-1.36 (m, 7H), 1.34-1.25 (m, 3H),1.24-1.14 (m, 9H), 1.14-0.82 (m, 4H), 0.80 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₃₂H₄₅N₂O₃ [M+H]⁺ 505, found 505.

Example 44: Synthesis of2-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1-oxoisoindoline-5-carbonitrile(Fit)

A mixture of D2 (345 mg, 1.65 mmol), E2 (300 mg, 0.825 mmol), and K₂CO₃(340 mg, 2.47 mmol) in EtOH (15 mL) was stirred at 25° C. for 1 h. Afterstirring at 95° C. for 72 h, the reaction mixture was diluted with DCM(100 mL), washed with water (100 mL), brine (100 mL), dried over Na₂SO₄,filtered and concentrated under vacuum to give F11 (500 mg) as a solid,which was purified by prep-HPLC (Condition: water (0.225% FA)-ACN, BeginB: 69, End B: 99, Gradient Time(min): 7, 100% B Hold Time(min): 1,FlowRate (ml/min): 25) to give F11 (95 mg, 19%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.93 (m, 1H), 7.81-7.71 (m, 2H), 4.63-4.33(m, 3H), 3.45-3.26 (m, 5H), 2.59 (s, 1H), 1.90-1.65 (m, 6H), 1.57-1.35(m, 7H), 1.34-1.27 (m, 2H), 1.24-1.09 (m, 8H), 1.08-0.84 (m, 4H), 0.79(s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₁H₄₃N₂O₃ [M+H]⁺ 491,found 491.

Example 45: Synthesis of2-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1-oxo-1,2,3,4-tetrahydroisoguinoline-6-carbonitrile(F13)

Synthesis of F12

To a solution of B18 (300 mg, 0.938 mmol) and benzoic acid,4-cyano-2-(2-oxoethyl)-, methyl ester (379 mg, 1.87 mmol) in DCE (6 mL)and CH₃OH (6 mL) was added NaCNBH₃ (176 mg, 2.81 mmol) and acetic acid(168 mg, 2.81 mmol) at 25° C. under N₂. After stirring at rt for 16 h,the mixture was poured into water (20 mL) and extracted with DCM (3×20mL). The combined organic solution was washed with brine (2×20 mL),dried over anhydrous Na₂SO₄, filtered and concentrated to give F12 (300mg) as an oil, used directly for the next step.

Synthesis of F13

A solution of F12 (300 mg, 0.592 mmol) in toluene (20 mL) was stirred at110° C. for 16 h. The reaction mixture was concentrated and purified byHPLC (Column Xtimate C18 150*25 mm*5 um Condition water (0.225% FA)-ACNBegin B 80 End B 100 Gradient Time(min) 7 100% B Hold Time(min) 2;FlowRate (ml/min) 25) to afford F13 (72 mg, 26%) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 8.19 (d, J=8.1 Hz, 1H), 7.62 (d, J=8.0 Hz,1H), 7.48 (s, 1H), 4.93 (br s, 1H), 3.72-3.31 (m, 2H), 3.19-3.01 (m,1H), 2.91 (br d, J=16.1 Hz, 1H), 1.87-1.62 (m, 7H), 1.54-1.21 (m, 14H),1.18-0.88 (m, 9H), 0.79 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₃₁H₄₃N₂O₂ [M+H]⁺ 475, found 475.

Example 46: Synthesis of2-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1-oxo-1,2,3,4-tetrahydroisoquinoline-6-carbonitrile(15)

Synthesis of F14

To a solution of F8 (100 mg, 1.23 mmol) and benzoic acid,4-cyano-2-(2-oxoethyl)-, methyl ester (250 mg, 1.23 mmol) in CH₃OH/DCE(2/2 mL) was added acetic acid (88.2 mg, 1.47 mmol) and NaBH₃CN (92.3mg, 1.47 mmol) in one portion at 25° C. under N₂. After stirring at rtfor 16 h, the reaction was combined with another batch prepared from 100mg of F8 and poured into aqueous NaHCO₃ (20 mL). The aqueous solutionwas extracted with DCM (2×50 mL). The combined organic solution waswashed with brine (2×30 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated to give F14 (600 mg) as an oil. LC-ELSD/MS purity 82%, MSESI calcd. for C₃₀H₅₁N₂O₄ [M+H]⁺ 551, found 551.

Synthesis of F15

A solution of F14 (580 mg, 1.05 mmol) in toluene (20 mL) was refluxedfor 16 h. The reaction mixture was concentrated and purified by HPLC(Column Xtimate C18 150*25 mm*5 um Condition water (0.225% FA)-ACN BeginB 90 End B 100 Gradient Time (min) 7 100% B Hold Time (min) 0; FlowRate(ml/min) 30) to afford F15 (42 mg, 8%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.19 (d, J=7.6 Hz, 1H), 7.62 (d, J=7.6 Hz,1H), 7.48 (s, 1H), 5.00-4.90 (m, 1H), 3.59-3.30 (m, 6H), 3.17-3.02 (m,1H), 2.98-2.91 (m, 1H), 0.76-0.70 (m, 1H), 1.83-1.70 (m, 4H), 1.68-1.54(m, 8H), 1.43-1.24 (m, 7H), 1.25-1.15 (m, 4H), 1.15-1.07 (m, 6H),1.15-0.90 (m, 1H), 0.79 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₃₃H₄₇N₂O₃ [M+H]⁺ 519, found 519.

Examples 47 & 48: Synthesis of(3R,5R,8R,9R,10S,13R,14S,17R)-3-(methoxymethyl)-13-methyl-17-(2-(5-methyl-2H-tetrazol-2-yl)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(G5) &(3R,5R,8R,9R,10S,13R,14S,17R)-3-(methoxymethyl)-13-methyl-17-(2-(5-methyl-1H-tetrazol-1-yl)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(G6)

Synthesis of G1

To a stirred solution of sodium hydride (1.23 g, 30.8 mmol, 60% in oil)in THF (75 mL) and was added ethyl 2-(diethoxyphosphanyl) (7.32 g, 32.7mmol) at 40° C. After stirring for 30 min under N₂, A33 (3.0 g, 9.4mmol) was added. After stirring at 65° C. for 4 h, the mixture wascooled and concentrated under reduced pressure at 40° C. The mixture waspoured into ice-water (100 mL) and extracted with EtOAc (3×100 mL). Thecombined organic solution was washed with saturated brine (2×100 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜20% of EtOAc in PE) to give G1 (2.9 g, 79%)as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.51 (t, J=2.4 Hz, 1H), 4.19-4.05 (m, 2H),3.43-3.29 (m, 5H), 2.85-2.78 (m, 2H), 2.60 (s, 1H), 1.98-1.75 (m, 7H),1.68-1.00 (m, 15H), 0.88-0.84 (m, 2H), 0.83-0.78 (m, 3H)

Synthesis of G2

To a solution of G1 (2.9 g, 7.42 mmol) in EtOH (50 mL) was addedPd—C(wet, 10%, 3 g) under N₂. The suspension was degassed under vacuumand purged with H₂ for three times. The mixture was stirred under H₂ (15psi) at 25° C. for 12 h to give a suspension. The reaction mixture wasfiltered through a pad of Celite and washed with EtOH (3×50 mL). Thefiltrate was concentrated to give G2 (2.7 g), used directly for the nextstep. LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₄H₃₉O₃[M−H₂O+H]⁺375,found 375.

Synthesis of G3

To a solution of G2 (2.7 g, 6.9 mmol) in THF (50 mL) was added lithiumaluminum hydride (390 mg, 10.3 mmol) in one portion at 20° C. under N₂.After stirring at 20° C. for 12 h, H₂O (2 mL) was added and 1 M HCl wasadded until pH to 5. The aqueous solution was extracted with EtOAc (3×10mL). The combined organic solution was washed with saturated brine (2×20mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜50% of EtOAc in PE) to give G3 (2.2 g,92%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.73-3.57 (m, 2H), 3.43-3.35 (m, 5H), 2.59(s, 1H), 1.90-1.71 (m, 7H), 1.52-1.19 (m, 13H), 1.18-0.97 (m, 7H), 0.59(s, 3H).

Synthesis of G4

To a solution of G3 (2.2 g, 6.3 mmol) in CH₂Cl₂ (20 mL) at 0° C. wereadded PPh₃ (1.9 g, 7.5 mmol) and NBS (1.3 g, 7.5 mmol). After stirringat rt for 4 h, the reaction mixture was diluted with water (100 mL) andextracted with EtOAc (3×30 mL). The combined organic solution was washedwith saturated brine (2×20 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜15% ofEtOAc in PE) to give G4 (1.1 g, 30%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.51-3.26 (m, 7H), 2.58 (s, 1H), 2.01-1.53(m, 12H), 1.49-0.94 (m, 14H), 0.59 (s, 3H).

Synthesis of G5 & G6

To a solution of G4 (250 mg, 0.6 mmol) in DMF (5 mL) was added Cs₂CO₃(390 mg, 1.2 mmol) and 5-methyl-2H-1,2,3,4-tetrazole (100 mg, 1.2 mmol).After stirring at 85° C. for 12 h, the reaction mixture was diluted withwater (100 mL) and extracted with EtOAc (3×20 mL). The combined organicsolution was washed with brine (2×50 mL), dried over Na₂SO₄, filtered,concentrated and purified by flash column (0˜30% of EtOAc in DCM) togive G5 (90 mg, 45%) as a solid and G6 (40 mg, 20%) as a solid.

G5: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.61-4.44 (m, 2H), 3.44-3.36 (m, 5H),2.56 (s, 1H), 2.53 (s, 3H), 2.16-2.07 (m, 1H), 1.90-1.61 (m, 9H),1.50-0.97 (m, 16H), 0.61 (s, 3H). The structure was confirmed by HMBC.LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₄H₃₉N₄O [M−H₂O+H]⁺399, found399.

G6: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.22 (t, J=8.0 Hz, 2H), 3.45-3.34 (m,5H), 2.64-2.55 (m, 4H), 2.06-1.96 (m, 1H), 1.87-1.62 (m, 9H), 1.51-1.01(m, 16H), 0.60 (s, 3H). The structure was confirmed by HMBC. LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₄H₃₉N₄O [M−H₂O+H]⁺399, found 399.

Examples 49 & 50: Synthesis of(3R,5R,8R,9R,10S,13R,14S,17R)-17-(2-(2H-1,2,3-triazol-2-yl)ethyl)-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(G7) &(3R,5R,8R,9R,10S,13R,14S,17R)-17-(2-(1H-1,2,3-triazol-1-yl)ethyl)-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(G8)

To a solution of G4 (200 mg, 0.48 mmol) in DMF (5 mL) was added Cs₂CO₃(315 mg, 0.97 mmol) and 2H-1,2,3-triazole (66.8 mg, 0.97 mmol). Afterstirring 85° C. for 12 h, the mixture was diluted with water (100 mL)and extracted with EtOAc (3×20 mL). The combined organic solution waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated, and purified by flash column (0˜30% of EtOAc in DCM) togive G7 (82 mg, 41%) as a solid and G8 (40 mg, 32%, Rf=0.20,PE/EtOAc=3/1) as a solid.

G7: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58 (s, 2H), 4.52-4.35 (m, 2H),3.42-3.35 (m, 5H), 2.58 (s, 1H), 2.16-2.05 (m, 1H), 1.85-1.68 (m, 6H),1.59-0.98 (m, 19H), 0.60 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₄H₄₀N₃O₂ [M+H]⁺ 402, found 402.

G8: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70 (s, 1H), 7.53 (s, 1H), 4.46-4.27(m, 2H), 3.45-3.29 (m, 5H), 2.57 (s, 1H), 2.09-2.00 (m, 1H), 1.88-1.80(m, 2H), 1.69-1.59 (m, 8H), 1.42-1.01 (m, 15H), 0.60 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₄H₄₀N₃O₂ [M+H]⁺ 402, found 402.

Examples 51 & 52: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(H7) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(H8)

Synthesis of H1

To a mixture of MePPh₃Br (16.6 g, 46.7 mmol) in THF (150 mL) was addedt-BuOK (5.24 g, 46.7 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 min. B1 (5 g, 15.6 mmol) was added. After stirring at 50° C. for2 h, the reaction mixture was quenched with 10% NH₄Cl aqueous (300 mL)at 25° C. and extracted with EtOAc (2×200 mL). The combined organicsolution was concentrated and triturated with MeOH/H₂O (1:1, 300 mL) togive H1 (4.8 g, 97.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.03 (t, J=9.2Hz, 1H), 1.90-1.78 (m, 4H), 1.75 (s, 3H), 1.72-1.59 (m, 5H), 1.50-1.24(m, 12H), 1.23-0.98 (m, 6H), 0.57 (s, 3H).

Synthesis of H2

To a solution of H₁ (4.8 g, 15.1 mmol) in THF (100 mL) was added 9-BBNdimer (7.3 g, 30.2 mmol). After stirring at 45° C. for 16 h, ethanol (10mL) at 15° C., followed by NaOH aqueous (30.1 mL, 5.0 M, 151 mmol) wereadded at 0° C. Hydrogen peroxide (15 mL, 10 M, 151 mmol) was then addeddropwise at 0° C. After stirring at 78° C. for 1 h, the mixture ascooled to 15° C. and water (150 mL) was added. After stirring at 25° C.for 20 min, the solid was filtered and washed with water (2×10 mL),dried under vacuum to give H2 (4.3 g).

Note: The ratio of 21-α-Me and 21-β-Me is 4:1 based on H-NMR.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.59 (m, 1H), 3.48-3.30 (m, 1H), 1.96(td, J=3.2, 12.4 Hz, 1H), 1.89-1.76 (m, 4H), 1.58-1.34 (m, 8H),1.33-1.14 (m, 12H), 1.09-0.93 (m, 8H), 0.68 (s, 3H).

Synthesis of H3

To a solution of H₂ (2 g, 5.97 mmol) in DCM (20 mL) was added DMP (5.04g, 11.9 mmol) in portions. After stirring at 25° C. for 30 min, themixture was quenched by saturated NaHCO₃ aqueous (200 mL), The aqueoussolution was extracted with DCM (2×150 mL). The combined organicsolution was washed with saturated Na₂S₂O₃ aqueous (200 mL), brine (200mL) dried over Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜20% of EtOAc in PE) to give H3 (1 g, 50.5%)as an oil.

Note: The ratio of 21-α-Me and 21-β-Me is 4:1 based on H-NMR.

¹H NMR (400 MHz, CDCl₃) δ_(H) 9.56 (d, J=3.2 Hz, 0.8H), 9.52 (d, J=3.2Hz, 0.2H), 2.59-2.17 (m, 2H), 2.04 (s, 1H), 1.94-1.80 (m, 5H), 1.68-1.60(m, 4H), 1.50-1.27 (m, 12H), 1.14-1.03 (m, 8H), 0.71-0.66 (m, 3H).

Synthesis of H4

To a solution of H3 (1 g, 3 mmol) in THF (20 mL) was added TsOH (1.03 g,6 mmol). After stirring at 25° C. for 16 h, the mixture was added H₂O(100 mL) and extracted with EtOAc (2×100 mL). The combined organicsolution was washed with saturated NaHCO₃ (200 mL), brine (200 mL),dried over anhydrous Na₂SO₄, filtered and concentrated to give H4 (1 g),which was used as is.

Note: The ratio of 21-α-Me and 21-β-Me is 2:3 based on H-NMR.

¹H NMR (400 MHz, CDCl₃) δ_(H) 9.56 (d, J=3.2 Hz, 0.4H), 9.52 (d, J=4.8Hz, 0.6H), 2.59-2.18 (m, 2H), 1.94-1.80 (m, 5H), 1.68-1.61 (m, 5H),1.46-1.24 (m, 12H), 1.12-1.02 (m, 8H), 0.71-0.66 (m, 3H).

Synthesis of H5

To a solution of H₄ (1 g) in MeOH (10 mL) was added NaBH₄ (226 mg, 6mmol). After stirring at 25° C. for 16 h, the reaction mixture wasquenched by saturated NH₄Cl (150 mL) and extracted with EtOAc (3×100mL). The combined organic solution was washed with brine (200 mL), driedover anhydrous Na₂SO4, filtered and concentrated to give H5 (1 g) as asolid, which was used as is.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.79-3.58 (m, 1H), 3.49-3.33 (m, 1H),1.96-1.78 (m, 5H), 1.55-1.35 (m, 9H), 1.33-1.17 (m, 11H), 1.09-0.92 (m,8H), 0.68 (s, 3H).

Synthesis of H6

To a solution of H5 (1 g, 2.98 mmol) in DCM (10 mL) at 0° C. was addedPPh₃ (936 mg, 3.57 mmol) and NBS (635 mg, 3.57 mmol). After stirring at25° C. for 1 h, the reaction mixture was poured into water (50 mL) andextracted with DCM (3×50 mL). The combined organic solution was washedwith saturated brine (100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtOAcin PE) to give H6 (640 mg, 54.2%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.63 (dd, J=2.8, 9.6 Hz, 0.6H), 3.50 (dd,J=2.8, 9.6 Hz, 0.4H), 3.41-3.29 (m, 1H), 1.95-1.75 (m, 5H), 1.69-1.60(m, 4H), 1.45-1.23 (m, 14H), 1.11-0.96 (m, 9H), 0.69-0.67 (m, 1H).

Synthesis of H7 & H8

To a solution of H6 (640 mg, 1.61 mmol) in acetone (10 mL) was addedCs₂CO₃ (1.58 g, 4.83 mmol) and 1H-pyrazole-4-carbonitrile (224 mg, 2.41mmol). After stirring at 55° C. for 12 h, the reaction mixture was addedwater (100 mL) and extracted with EtOAc (2×80 mL). The combined organicsolution dried over Na₂SO₄, filtered, concentrated and purified by flashcolumn (0˜25% of EtOAc in PE) to give a mixture of H7 & H8 (500 mg) asoil. The diastereomers (350 mg, 0.85 mmol) were separated by SFC(Column:DAICEL CHIRALPAK AS-H (250 mm*30 mm, 5 um); Condition: 0.1%NH₃H₂O ETOH; Begin B: 30%; End B: 30%; FlowRate(ml/min): 65) to give H7(156 mg, 44.6%) and H8 (120 mg, 34.3%), both as solids.

H7: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.49 (dd,J=4.4, 13.6 Hz, 1H), 3.66 (dd, J=10.8, 13.2 Hz, 1H), 2.17-2.04 (m, 1H),1.91-1.73 (m, 5H), 1.70-1.60 (m, 3H), 1.50-1.25 (m, 13H), 1.22-1.00 (m,7H), 0.79 (s, 3H), 0.68 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%,analytic SFC: 100% de, MS ESI calcd. for C₂₆H₃₉N₃O [M+H]⁺ 410.3, found410.3.

H8: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.25 (dd,J=4.0, 13.2 Hz, 1H), 3.72 (dd, J=9.6, 13.6 Hz, 1H), 2.07-1.77 (m, 6H),1.70-1.60 (m, 3H), 1.50-1.25 (m, 13H), 1.21-1.00 (m, 7H), 0.81 (d, J=6.4Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, analytic SFC: 97.08% de,MS ESI calcd. for C₂₆H₃₉N₃O [M+H]⁺ 410.3, found 410.3.

Examples 53-56: Synthesis of(3R,5S,8R,9R,10S,13S,14S,17R)-3-(methoxymethyl)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H₂O),(3R,5S,8R,9R,10S,13S,14S,17R)-3-(methoxymethyl)-13-methyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H21),(3R,5S,8R,9R,10S,13S,14S,17R)-3-(methoxymethyl)-13-methyl-17-((R)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H22) &(3R,5S,8R,9R,10S,13S,14S,17R)-3-(methoxymethyl)-13-methyl-17-((S)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H23)

Synthesis of H10

To a solution of trimethylsulfoxonium iodide (4.2 g, 19.1 mmol) in DMSO(50 mL) was added t-BuOK (2.14 g, 19.1 mmol). After stirring at 60° C.for 1 h under N₂, (5α)-estrane-3,17-dione (5 g, 18.2 mmol, CAS:5696-58-2) was added. After stirring at 25° C. for 2 h, the reaction wasdiluted with water (200 mL) and extracted with EtOAc (2×200 mL). Thecombined organic solution was washed with brine (300 mL), dried overanhydrous Na₂SO₄, filtered, and concentrated in vacuum to afford H10 (5g, 95.4%) as a solid

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.67-2.61 (m, 2H), 2.44 (dd, J=8.4, 19.2Hz, 1H), 2.13-2.03 (m, 1H), 2.00-1.74 (m, 6H), 1.70-1.61 (m, 2H),1.55-1.40 (m, 2H), 1.38-0.99 (m, 9H), 0.92-0.71 (m, 5H).

Synthesis of H11

To anhydrous MeOH (100 mL) was added Na (1.19 g, 51.9 mmol) at 25° C. inportions. After 30 min, H10 (5 g, 17.3 mmol) was added. After stirringat 60° C. for 16 h, the reaction was diluted with water (200 ml) andconcentrated to remove most of the solvent. The mixture was extractedwith EtOAc (2×200 mL). The combined organic solution was washed withbrine (2×100 mL), dried over anhydrous Na₂SO₄, filtered, concentrated togive H11 (5.5 g, 99.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.38 (s, 3H), 3.18 (s, 2H), 2.43 (dd,J=8.4, 19.2 Hz, 1H), 2.12-2.03 (m, 1H), 1.96-1.72 (m, 6H), 1.64-1.45 (m,5H), 1.36-1.19 (m, 5H), 1.15-0.97 (m, 4H), 0.87 (s, 3H), 0.80-0.68 (m,2H).

Synthesis of H12

To a mixture of EtPPh₃Br (19.0 g, 51.3 mmol) in THF (150 mL) was addedt-BuOK (5.75 g, 51.3 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 min, H11 (5.5 g, 17.1 mmol) was added in portions below 50° C.After stirring at 40° C. for 2 h, the reaction mixture was quenched with10% NH₄Cl aqueous (300 mL) at 25° C. and extracted with EtOAc (2×200mL). The combined organic solution was concentrated and purified bytrituration with MeOH/H₂O (1:1, 150 mL) to give H12 (5 g, 88.0%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-5.06 (m, 1H), 3.38 (s, 3H), 3.18 (s,2H), 2.42-2.30 (m, 1H), 2.26-2.13 (m, 2H), 2.07-1.97 (m, 1H), 1.86-1.63(m, 8H), 1.58-1.36 (m, 4H), 1.27-0.95 (m, 9H), 0.87 (s, 3H), 0.78-0.65(m, 2H).

Synthesis of H13

To a solution of H12 (5 g, 15.0 mmol) in THF (100 mL) was added BH₃.Me₂S(7.5 mL, 10 M, 75.0 mmol). After stirring at 25° C. for 2 h, EtOH (10mL) followed by NaOH (30 mL, 5 M) and H₂O₂ (15 mL, 10 M) were addeddropwise. After stirring at 60° C. for 1 h, the mixture was quenched byNa₂S₂O₃ (400 mL, 10%) and extracted with EtOAc (2×300 mL). The combinedorganic solution was washed with brine (300 mL), dried over Na₂SO4,filtered and concentrated to give H13 (5.25 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.86-3.78 (m, 1H), 3.73-3.61 (m, 1H), 3.36(s, 3H), 3.16 (s, 2H), 2.17-1.96 (m, 1H), 1.89-1.66 (m, 7H), 1.52-1.34(m, 5H), 1.19 (d, J=6.4 Hz, 3H), 1.16-0.83 (m, 10H), 0.81-0.56 (m, 5H).

Synthesis of H14

To a solution of H13 (5.25 g, 14.9 mmol) in DCM (100 mL) was added DMP(12.6 g, 29.8 mmol) in portions. After stirring at 25° C. for 1 h, themixture was quenched by saturated NaHCO₃ aqueous (300 mL) and extractedwith DCM (2×250 mL). The combined organic solution was washed withsaturated Na₂S₂O₃ aqueous (400 mL), brine (300 mL) dried over Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜15% of EtOAc in PE) to give H14 (4 g, 77.0%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.38 (s, 3H), 3.18 (s, 2H), 2.53 (t, J=8.8Hz, 1H), 2.19-2.12 (m, 1H), 2.11 (s, 3H), 2.02-1.94 (m, 1H), 1.87-1.68(m, 4H), 1.64-1.54 (m, 4H), 1.48-1.35 (m, 2H), 1.29-0.92 (m, 10H),0.81-0.64 (m, 2H), 0.61 (s, 3H).

Synthesis of H15

To a mixture of MePPh₃Br (12.2 g, 34.2 mmol) in THF (100 mL) was addedt-BuOK (3.83 g, 34.2 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 min, H14 (4 g, 11.4 mmol) was added. After stirring at 50° C. for3 h, the reaction mixture was quenched with 10% NH₄Cl aqueous (300 mL)at 25° C. and extracted with EtOAc (2×300 mL). The combined organicsolution was concentrated. The residue was purified by trituration withMeOH/H₂O (1:1, 200 mL) to give H15 (3.5 g, 88.6%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H), 3.38 (s, 3H),3.18 (s, 2H), 2.08-2.01 (m, 1H), 1.85-1.68 (m, 9H), 1.62-1.34 (m, 4H),1.26-0.94 (m, 11H), 0.75-0.63 (m, 2H), 0.56 (s, 3H).

Synthesis of H16

To a solution of H15 (3.5 g, 10.0 mmol) in THF (80 mL) was addedBH₃.Me₂S (5.0 mL, 10 M, 50.0 mmol). After stirring at 25° C. for 1 h,EtOH (10 mL) followed by NaOH (20 mL, 5 M) and H₂O₂ (10 mL, 10 M) wereadded dropwise. After stirring at 60° C. for 1 h, the mixture wasextracted with EtOAc (2×200 mL), washed with Na₂S₂O₃ (300 mL, 10%),brine (150 mL), dried over Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜30% of EtOAc in PE) to H16 (3.1g, 85.1%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H).3.71-3.53 (m, 1H), 3.43-3.24 (m, 1H), 3.32(s, 3H), 3.11 (s, 2H), 1.98-1.85 (m, 1H), 1.81-1.61 (m, 5H), 1.54-1.45(m, 4H), 1.39-1.04 (m, 8H), 1.02-0.73 (m, 10H), 0.88-0.55 (m, 5H).

Synthesis of H17

To a solution of H16 (600 mg, 1.64 mmol) in DCM (8 mL) at 0° C. wasadded PPh₃ (514 mg, 1.96 mmol) and NBS (348 mg, 1.96 mmol). Afterstirring at 25° C. for 2 h, the reaction mixture was diluted with water(100 mL) and extracted with DCM (2×80 mL). The combined organic solutionwas washed with brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give H17 (700 mg, 99.8%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.68-3.47 (m, 1H), 3.41-3.30 (m, 4H), 3.18(s, 2H), 2.03-1.58 (m, 10H), 1.45-1.11 (m, 7H), 1.11-0.88 (m, 10H),0.75-0.62 (m, 5H).

Synthesis of H18 & H19

To a solution of H17 (300 mg, 0.71 mmol) in DMF (8 mL) was added Cs₂CO₃(684 mg, 2.1 mmol) and 5-methyl-2H-1,2,3,4-tetrazole (88.2 mg, 1.05mmol). After stirring at 85° C. for 4 h, the reaction mixture wasdiluted with water (200 mL) and extracted with EtOAc (2×150 mL). Thecombined organic solution was dried over Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (5-60% of EtOAcin PE) to give H18 (200 mg, 66.2%) and H₁₉ (90 mg, 29.8%, Rf=0.1(PE:EtOAc=3:1)) both as solids.

H18: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.82-4.46 (m, 1H), 4.33-4.18 (m, 1H),3.38 (s, 3H), 3.18 (s, 2H), 2.53 (s, 3H), 2.29-2.07 (m, 1H), 2.04-1.59(m, 9H), 1.55-1.19 (m, 6H), 1.18-0.88 (m, 8H), 0.88-0.80 (m, 3H),0.77-0.65 (m, 5H).

H19: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.63-4.20 (m, 1H), 3.93-3.78 (m, 1H),3.39 (s, 3H), 3.18 (s, 2H), 2.54 (s, 3H), 2.18-1.84 (m, 4H), 1.82-1.60(m, 6H), 1.47-1.36 (m, 2H), 1.30-0.91 (m, 12H), 0.85-0.79 (m, 3H),0.77-0.64 (m, 5H).

Synthesis of H2O & H21

H18 (200 mg, 0.46 mmol) was separated into C21 diastereomers by SFC(Column: DAICEL CHIRALPAK IC (250 mm*30 mm, 5 um); Condition: 0.1%NH₃H₂O IPA; Begin B: 45%; End B: 45%; FlowRate(ml/min): 50) to give H₂O(62 mg, 31.1%) and H21 (104 mg, 52.2%) both as solids. Theconfigurations of C20 in these two compounds were referred to the paper“Chem. Rev. 2014, 114, 6349-6382”. The peak of C21-β-Me in H-NMR is inhigher field than C21-α-Me.

H20: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.76 (dd, J=4.4, 13.2 Hz, 1H), 4.23(dd, J=10.4, 13.2 Hz, 1H), 3.38 (s, 3H), 3.18 (s, 2H), 2.53 (s, 3H),2.29-2.15 (m, 1H), 2.01 (s, 1H), 1.93-1.62 (m, 7H), 1.59-1.55 (m, 2H),1.47-1.19 (m, 6H), 1.15-0.93 (m, 7H), 0.81 (s, 3H), 0.76-0.65 (m, 5H);LC-ELSD/MS purity 99%; analytical SFC: 96.46%; MS ESI calcd. forC₂₅H₄₂N₄O₂ [M+H]⁺ 431.3, found 431.3.

H21: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.52 (dd, J=4.0, 13.2 Hz, 1H), 4.28(dd, J=9.2, 13.2 Hz, 1H), 3.38 (s, 3H), 3.18 (s, 2H), 2.53 (s, 3H),2.20-2.07 (m, 1H), 2.02-1.87 (m, 3H), 1.77-1.58 (m, 7H), 1.48-1.36 (m,2H), 1.26-0.95 (m, 11H), 0.85 (d, J=6.8 Hz, 3H), 0.76-0.63 (m, 5H).LC-ELSD/MS purity 99%; analytical SFC: 95.38%; MS ESI calcd. forC₂₅H₄₂N₄O₂ [M+H]⁺ 431.3, found 431.3.

Synthesis of H22 & H23

H19 (90 mg, 0.46 mmol) was separated into C21 diastereomers by prep-HPLC(Column:Xtimate C18 150*25 mm*5 um; Condition: water (0.04% NH₃H₂O+10 mMNH₄HCO₃)— ACN; Begin B: 55%; End B: 85%; Gradient Time(min):7.5; 100% BHold Time(min):2; FlowRate(ml/min): 30) to give H22 (27 mg, 30%) and H₂₃(12 mg, 13.3%)(P1) both as solids. The configurations of C20 in thesetwo compounds were referred to the paper “Chem. Rev. 2014, 114,6349-6382”. The peak of C21-β-Me in H-NMR is in higher field thanC21-α-Me.

H22: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.54 (dd, J=4.4, 14.0 Hz, 1H), 3.83(dd, J=11.2, 13.6 Hz, 1H), 3.39 (s, 3H), 3.19 (s, 2H), 2.55 (s, 3H),2.19-2.07 (m, 1H), 2.01 (s, 1H), 1.92-1.60 (m, 8H), 1.48-1.18 (m, 7H),1.16-0.94 (m, 7H), 0.82 (s, 3H), 0.76-0.64 (m, 5H); LC-ELSD/MS purity99%; 100% de based on H-NMR; MS ESI calcd. for C₂₅H₄₂N₄O₂ [M+H]⁺ 431.3,found 431.3.

H23: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.28 (dd, J=3.6, 13.6 Hz, 1H), 3.86(dd, J=10.4, 13.6 Hz, 1H), 3.38 (s, 3H), 3.18 (s, 2H), 2.54 (s, 3H),2.15-1.79 (m, 4H), 1.77-1.64 (m, 5H), 1.60-1.55 (m, 2H), 1.47-1.36 (m,2H), 1.29-0.95 (m, 11H), 0.82 (d, J=6.8 Hz, 3H), 0.76-0.65 (m, 5H);LC-ELSD/MS purity 99%; 100% de based on H-NMR; MS ESI calcd. forC₂₅H₄₂N₄O₂ [M+H]⁺ 431.3, found 431.3.

Example 57-60: Synthesis of(3R,5S,8R,9R,10S,13S,14S,17R)-17-((R)-1-(2H-1,2,3-triazol-2-yl)propan-2-yl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H33),(3R,5S,8R,9R,10S,13S,14S,17R)-17-((S)-1-(2H-1,2,3-triazol-2-yl)propan-2-yl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H34),(3R,5S,8R,9R,10S,13S,14S,17R)-17-((R)-1-(1H-1,2,3-triazol-1-yl)propan-2-yl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H35) &(3R,5S,8R,9R,10S,13S,14S,17R)-17-((S)-1-(1H-1,2,3-triazol-1-yl)propan-2-yl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(H36)

Synthesis of H24

A suspension of LiCl (12.9 g, 305 mmol, anhydrous) in THF (600 mL,anhydrous) was stirred at 25° C. under N₂. After 30 mins, FeCl3 (19.4 g,120 mmol, anhydrous) was added and the mixture was cooled to −30° C.before adding MeMgBr (145 mL, 3M in diethyl ether, 436 mmol) dropwise at−30° C. After stirring at −30° C. for 10 mins, H₉ (20 g, 72.8 mmol) wasadded. After stirring at −15° C. for 2 h, citric acid (500 mL, 10% aq.)was added and the mixture was extracted with EtOAc (2×300 mL). Thecombined organic solution was washed with saturated brine (300 mL),dried over anhydrous Na₂SO₄, filtered and concentrated in vacuum to giveH24 (20 g, 95%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.43 (dd, J=8.8, 19.2 Hz, 1H), 2.14-2.00(m, 1H), 1.97-1.85 (m, 2H), 1.81-1.72 (m, 3H), 1.71-1.44 (m, 5H),1.40-1.16 (m, 8H), 1.15-0.98 (m, 5H), 0.87 (s, 3H), 0.80-0.64 (m, 2H)

Synthesis of H25

To a mixture of PPh₃EtBr (50.8 g, 137 mmol) in THF (200 mL) was addedt-BuOK (15.3 g, 137 mmol) at 25° C. under N₂. After stirring at 40° C.for 30 min, H24 (20 g, 68.6 mmol) was added. After stirring at 40° C.for 3 h, the reaction mixture was quenched with saturated NH₄Cl aqueous(300 mL) at 20° C. and extracted with EtOAc (2×500 mL). The combinedorganic solution was concentrated and purified by trituration withMeOH/H₂O (1:1, 1 L) at reflux to give H25 (20 g, 96.1%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-5.05 (m, 1H), 2.43-2.09 (m, 3H),1.86-1.47 (m, 10H), 1.47-1.40 (m, 2H), 1.38-1.27 (m, 2H), 1.22-0.94 (m,11H), 0.88 (s, 3H), 0.77-0.60 (m, 2H)

Synthesis of H26

To a solution of H25 (20.0 g, 66.1 mmol) in anhydrous THF (200 mL) wasadded 9-BBN dimer (48.3 g, 198 mmol) at 25° C. under N₂. After stirringat 50° C. for 2 h, the mixture was cooled and quenched by EtOH (37.8 mL,660 mmol) at 0° C. NaOH (26.4 g in 132 mL water, 5 M, 660 mmol) was thenadded very slowly followed by H₂O₂ (66.0 mL, 10 M, 660 mmol) maintaininner temperature below 30° C. After stirring at 50° C. for 1 h, themixture was poured into sat. Na₂S₂O₃ (500 mL), stirred for 30 mins andextracted with EtOAc (2×300 mL). The combined organic solution was driedover Na₂SO₄, filtered and concentrated in vacuum to give H26 (50 g),which was purified by trituration in methanol/H₂O (1:1) (500 mL) at 25°C. for 16 h to give H26 (20 g, 40.3%) as a solid.

Synthesis of H27

To a solution of H26 (10 g, 31.1 mmol) in DCM (100 mL) was added DMP(65.7 g, 155 mmol) at 25° C. After stirring at 25° C. for 1 h, themixture was washed with a mixed solution of NaHCO₃ (500 mL, sat.) andNa₂S₂O₃ (2×500 mL, sat.), dried over Na₂SO₄, filtered, concentrated invacuum and purified by flash column (15-35% EtOAc in PE) to give H27 (7g, 70.7%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.53 (t, J=8.8 Hz, 1H), 2.23-1.94 (m, 6H),1.89-1.72 (m, 2H), 1.70-1.50 (m, 5H), 1.46-0.91 (m, 15H), 0.77-0.63 (m,2H), 0.61 (s, 3H).

Synthesis of H28

To a mixture of MePPh₃Br (6.71 g, 18.8 mmol) in THF (25 mL) was addedt-BuOK (2.10 g, 18.8 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 min, H27 (3.0 g, 9.4 mmol) was added. After stirring 50° C. for 2h, the reaction mixture was quenched with saturated NH₄Cl aqueous (50mL) at 25° C. and extracted with EtOAc (2×200 mL). The combined organicsolution was dried over Na₂SO₄, filtered, concentrated and purified byflash column (0˜20% of EtOAc in PE) to give H28 (2.2 g, 74.0%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s 1H), 4.70 (s, 1H), 2.08-1.99 (m,1H), 1.80-1.62 (m, 10H), 1.60-1.51 (m, 3H), 1.37-0.94 (m, 15H),0.75-0.62 (m, 2H), 0.57 (s, 3H)

Synthesis of H29

To a solution of H28 (2.2 g, 6.95 mmol) in THF (20 mL) was addedBH₃.Me₂S (3.46 mL, 10 M, 34.7 mmol). After stirring at 25° C. for 16 h,EtOH (4 mL, 69.5 mmol) was added dropwise followed by NaOH (2.77 g in13.9 mL water, 69.5 mmol) and H₂O₂ (6.95 mL, 10 M, 69.5 mmol). Afterstirring at 60° C. for 3 h, the reaction mixture was quenched by Na₂SO₃(100 mL, 10%) and extracted with EtOAc (2×200 mL). The combined organicsolution was dried over Na₂SO₄, filtered and concentrated and purifiedby flash column (0˜30% of EtOAc in PE) to give H29 (2.7 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.83-3.57 (m, 1H), 3.50-3.30 (m, 1H),2.00-1.62 (m, 7H), 1.56-1.48 (m, 3H), 1.19 (s, 10H), 1.12-0.91 (m, 11H),0.68 (s, 5H)

Synthesis of H30

To a solution of H29 (500 mg, 1.5 mmol) in DCM (8 mL) at 0° C. was addedPPh₃ (584 mg, 2.2 mmol) and NBS (396 mg, 2.2 mmol). After stirring at25° C. for 2 h, the reaction mixture was diluted water (100 mL) andextracted with DCM (2×80 mL). The combined organic solution was washedwith saturated brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give H30 (400 mg, 67.5%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.67-3.47 (m, 1H), 3.40-3.30 (m, 1H),1.95-1.50 (m, 11H), 1.38-1.23 (m, 6H), 1.20 (s, 3H), 1.16-0.95 (m, 10H),0.71-0.60 (m, 5H)

Synthesis of H31-H36

To a solution of H30 (400 mg, 1.0 mmol) in DMF (10 mL) was added Cs₂CO₃(977 mg, 3.0 mmol) and 2H-1,2,3-triazole (103 mg, 1.5 mmol). Afterstirring at 80° C. for 16 h, the reaction mixture was diluted with water(50 mL) and extracted with EtOAc (2×50 mL). The combined organicsolution was dried over Na₂SO₄, filtered, concentrated and purified byflash column (5-90% of EtOAc in PE) to give H31 (170 mg, 44.1%) and H32(170 mg, 44.1%) both as solids

The C21 diastereomers of H₃₁ (170 mg) were separated by SFC (column:DAICEL CHIRALPAK ADH (250 mm*30 mm, 5 um), gradient: 40-40% B (water(0.1% NH₃H₂O IPA), flow rate: 50 mL/min) to give H33 (20 mg, 11.8%) andH34 (74 mg, 43.7%) both as solids.

H34: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58 (s, 2H), 4.52-4.44 (m, 1H),4.18-4.07 (m, 1H), 2.22-2.08 (m, 1H), 2.04-1.89 (m, 2H), 1.79-1.61 (m,5H), 1.53-1.26 (m, 4H), 1.21-0.90 (m, 15H), 0.81 (d, J=6.8 Hz, 3H), 0.72(s, 3H), 0.68-0.60 (m, 2H); LCMS 30-90AB_2 min_E. purity≥99%, MS ESIcalcd. for C₂₄H₄₀N₃O [M+H]⁺ 386.3, found 386.3.

H33: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58 (s, 2H), 4.75-4.67 (m, 1H),4.12-4.03 (m, 1H), 2.30-2.17 (m, 1H), 1.95-1.72 (m, 4H), 1.69-1.61 (m,3H), 1.53-1.51 (m, 1H), 1.39-1.17 (m, 10H), 1.13-0.91 (m, 8H), 0.82 (s,3H), 0.66 (d, J=6.8 Hz, 5H); LCMS 30-90AB_2 min_E. purity≥99%, MS ESIcalcd. for C₂₄H₄₀N₃O [M+H]⁺ 386.3, found 386.3.

The C21 diastereomers of H32 (170 mg) was purified by SFC (column:DAICEL CHIRALPAK AD (250 mm*30 mm, 10 um), gradient: 45-45% B (0.1%NH₃H₂O ETOH), flow rate: 70 mL/min) to give H35 (39 mg, 23.0%) and H36(36 mg, 21.3%) both as a solid.

H35: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70 (s, 1H), 7.50 (s, 1H), 4.72-4.63(m, 1H), 4.02-3.93 (m, 1H), 2.17-2.05 (m, 1H), 1.94-1.60 (m, 7H),1.54-1.51 (m, 1H), 1.40-1.18 (m, 10H), 1.16-0.97 (m, 8H), 0.82 (s, 3H),0.75-0.62 (m, 5H); LCMS 30-90AB_2 min_E. purity>99%, MS ESI calcd. forC₂₄H₄₀N₃O [M+H]⁺ 386.3, found 386.3.

H36: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70 (s, 1H), 7.50 (s, 1H), 4.46-4.38(m, 1H), 4.11-4.02 (m, 1H), 2.07-1.88 (m, 3H), 1.79-1.60 (m, 5H),1.55-1.24 (m, 5H), 1.21-0.92 (m, 13H), 0.84 (d, J=6.4 Hz, 3H), 0.72 (s,3H), 0.70-0.61 (m, 2H); LCMS 30-90AB_2 min_E. purity≥99%, MS ESI calcd.for C₂₄H₄₀N₃O [M+H]⁺ 386.3, found 386.3.

Examples 61-64: Synthesis of1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-ethyl-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(J8),1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-ethyl-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(J9),1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-ethyl-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(J10) &1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-ethyl-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(J11)

Synthesis of J2

To a solution of 2, 6-di-tert-butyl-4-methylphenol (22.5 g, 102 mmol) intoluene (50 mL) was added dropwise AlMe₃ (25.5 mL, 51.0 mmol, 2 M intoluene) at 0° C. The mixture was stirred at 25° C. for 1 h and useddirectly as MAD solution. To the MAD (51.0 mmol) was added a solution ofJ1 (5 g, 15.7 mmol) in DCM (25 mL) dropwise at −70° C. After stirring at−70° C. for 1 h under N₂, bromo(ethyl)magnesium (15.6 mL, 47.0 mmol, 3Min ethyl ether) was added dropwise at −70° C. The resulting solution wasstirred at −70° C. for another 1 h. The reaction mixture was poured intosaturated aqueous citric acid (50 mL) at below 10° C. followed by addingice-water (60 mL) and stirred for another 10 min. The aqueous solutionwas extracted with EtOAc (2×40 mL). The combined organic solution waswashed with brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (10% of EtOAc inPE) to give J2 (3.7 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.57-2.49 (m, 1H), 2.22-2.09 (m, 4H),2.03-1.96 (m, 1H), 1.94-1.79 (m, 2H), 1.73-1.62 (m, 3H), 1.61-1.33 (m,12H), 1.24-0.96 (m, 6H), 0.93 (s, 3H), 0.88 (t, J=7.6 Hz, 3H), 0.59 (s,3H).

Synthesis of J3

To a suspension of MePh₃PBr (3.07 g, 8.64 mmol) in THF (25 mL) was addedt-BuOK (967 mg, 8.64 mmol). After stirring at 40° C. for 10 min, themixture was slowly added dropwise to a solution of J2 (1.5 g, 4.32 mmol)in THF (15 mL) at 20° C. After stirring for 30 min, the reaction wasquenched with sat.NH₄Cl (50 mL) and extracted with EtOAc (3×50 mL). Thecombined organic solution was washed with sat. NH₄Cl (50 mL), dried overNa₂SO₄, filtered, concentrated and purified by combi-flash (0˜25% ofEtOAc in PE) to give J3 (1.2 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.07-1.98 (m,1H), 1.94-1.79 (m, 3H), 1.75 (s, 3H), 1.73-1.60 (m, 3H), 1.53-1.32 (m,8H), 1.29-1.14 (m, 9H), 0.93 (s, 3H), 0.90-0.86 (m, 5H), 0.55 (s, 3H).

Synthesis of J4

To a solution of J3 (1.2 g, 3.48 mmol) in THF (20 mL) was added BH₃/Me₂S(1.73 mL, 10 M, 17.4 mmol) dropwise at 25° C. under N₂. After stirringat 25° C. for 2 h, the mixture was cooled to 0° C. and treated with EtOH(1.60 g, 34.8 mmol), NaOH (6.95 mL, 5M, 34.8 mmol) and then H₂O₂ (3.47mL, 10 M, 34.8 mmol) slowly maintain inner temperature below 0° C. Whenthe inner temperature no longer rises, the mixture was poured into water(30 mL) and stirred for 30 mins. The suspension was extracted with EtOAc(2×30 mL). The combined organic solution was wash with water (2×30 mL),dried over anhydrous Na₂SO₄, filtered, concentrated and purified byflash column (0˜30% of EtOAc in PE) to give J4 (915 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.80-3.58 (m, 1H), 3.52-3.32 (m, 1H), 2.99(s, 2H), 1.99-1.77 (m, 4H), 1.73-1.57 (m, 4H), 1.51-1.33 (m, 7H),1.31-1.12 (m, 9H), 1.07-0.94 (m, 5H), 0.93 (s, 3H), 0.87 (t, J=7.2 Hz,3H), 0.66 (s, 3H).

Synthesis of J5

To a solution of J4 (458 mg, 1.26 mmol) in DCM (10 mL) at 0° C. wasadded PPh₃ (495 mg, 1.89 mmol) and NBS (336 mg, 1.89 mmol). Afterstirring at 25° C. for 2 h, the reaction was diluted with water (10 mL)and extracted with DCM (2×15 mL). The combined organic solution waswashed with brine (2×15 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtOAcin PE) to give J5 (440 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.66-3.47 (m, 1H), 3.40-3.28 (m, 1H),1.97-1.77 (m, 4H), 1.73-1.62 (m, 2H), 1.56-1.43 (m, 4H), 1.42-1.24 (m,11H), 1.23-0.95 (m, 9H), 0.93 (s, 3H), 0.87 (t, J=7.2 Hz, 3H), 0.66 (d,J=2.8 Hz, 3H).

Synthesis of J6 & J7

To a solution of J5 (390 mg, 0.916 mmol) in DMF (10 mL) was added Cs₂CO₃(594 mg, 1.83 mmol) and 1H-pyrazole-3-carbonitrile (170 mg, 1.83 mmol).After stirring at 85° C. for 12 h, the reaction was washed by water andaq. LiCl (30 mL, 5%), brine (30 mL), dried over anhydrous Na₂SO₄,filtered and concentrated under vacuum. The residue was purified byflash column (30% of EtOAc in PE) to give J6 (286 mg, 71%) and J7 (54mg, 13%) both as oils.

J6: LC-ELSD/MS MS ESI calcd for C₂₈H₄₂N₃[M−H₂O+H]⁺420, found 420;C₂₈H₄₃N₃ONa [M+Na]⁺460, found 460.

J7: LC-ELSD/MS MS ESI calcd. for C₂₈H₄₂N₃[M−H₂O+H]⁺420, found 420;C₂₈H₄₃N₃ONa [M+Na]⁺460, found 460.

Synthesis of J8 & J9

J6 (286 mg, 0.653 mmol) was purified by SFC (Column: Chiralcel OD-350×4.6 mm I.D., 3 um; Mobile solution: A: CO₂ B:ethanol (0.05% DEA)Gradient: from 5% to 40% of B in 2 min and hold 40% for 1.2 min, then 5%of B for 0.8 min; Flow rate: 4 mL/min) to afford J9 (72.7 mg, 25.5%) andJ8 (99.3 mg, 34.8%) as solids.

The C20-Me diastereomers were assigned based on ¹H NMR of C21-Me.

J8: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.39 (d, J=2.4 Hz, 1H), 6.65 (d, J=2.4Hz, 1H), 4.49 (dd, J=4.8, 13.3 Hz, 1H), 3.69 (dd, J=10.4, 13.3 Hz, 1H),2.18-2.05 (m, 1H), 1.93-1.78 (m, 4H), 1.72-1.57 (m, 4H), 1.51-1.33 (m,8H), 1.31-1.09 (m, 9H), 1.05-0.97 (m, 1H), 0.94 (s, 3H), 0.88 (t, J=7.6Hz, 3H), 0.77 (s, 3H), 0.67 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MSESI calcd. for C₂₈H₄₂N₃[M−H₂O+H]⁺420, found 420; C₂₈H₄₃N₃ONa [M+Na]⁺460,found 460. SFC 99.91% de.

J9: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.32 (d, J=2.4 Hz, 1H), 6.58 (d, J=2.4Hz, 1H), 4.20 (dd, J=4.0, 13.4 Hz, 1H), 3.67 (dd, J=10, 13.4 Hz, 1H),2.01-1.72 (m, 5H), 1.66-1.51 (m, 4H), 1.43-1.27 (m, 8H), 1.24-1.02 (m,9H), 0.97-0.89 (m, 1H), 0.86 (s, 3H), 0.81 (t, J=7.2 Hz, 3H), 0.73 (d,J=6.4 Hz, 3H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₈H₄₂N₃[M−H₂O+H]⁺420, found 420; C₂₈H₄₃N₃ONa [M+Na]⁺460, found 460;C₂₈H₄₃N₃ONa [M+Na]⁺460, found 460. SFC 100% de.

Synthesis of J10 & J11

J7 (54 mg, 0.123 mmol) was purified by SFC (Column: Chiralpak AD-350×4.6 mm I.D., 3 um; Mobile solution: A: CO₂ B:ethanol (0.05% DEA)Gradient: from 5% to 40% of B in 2 min and hold 40% for 1.2 min, then 5%of B for 0.8 min; Flow rate: 4 mL/min) to afford J10 (20.8 mg, 39%) andJ11 (17.2 mg, 32%) as solids.

The C20-Me diastereomers were assigned based on ¹H NMR of C21-Me.

J10: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.56 (d, J=2.0 Hz, 1H), 6.78 (d,J=2.0 Hz, 1H), 4.59 (dd, J=4.8, 13.2 Hz, 1H), 3.90 (dd, J=11.2, 13.2 Hz,1H), 2.19 (br d, J=9.6 Hz, 1H), 1.95-1.80 (m, 4H), 1.73-1.57 (m, 4H),1.51-1.34 (m, 7H), 1.32-1.08 (m, 10H), 1.05-0.96 (m, 1H), 0.94 (s, 3H),0.88 (t, J=7.6 Hz, 3H), 0.81 (s, 3H), 0.68 (d, J=6.4 Hz, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₈H₄₂N₃[M−H₂O+H]⁺420, found 420;C₂₈H₄₃N₃ONa [M+Na]⁺460, found 460. SFC 99.72% de.

J11: ¹H NMR (400 MHz, CDCl₃) δ_(H)=7.57 (d, J=2.4 Hz, 1H), 6.77 (d,J=2.0 Hz, 1H), 4.37 (dd, J=4.0, 13.4 Hz, 1H), 3.91 (dd, J=10.4, 13.6 Hz,1H), 2.19-2.05 (m, 1H), 1.99-1.79 (m, 4H), 1.72-1.57 (m, 4H), 1.51-1.34(m, 7H), 1.31-1.07 (m, 10H), 1.04-0.96 (m, 1H), 0.93 (s, 3H), 0.88 (t,J=7.2 Hz, 3H), 0.81 (d, J=6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₈H₄₂N₃[M−H₂O+H]⁺420, found 420; C₂₈H₄₄N₃O[M+H]⁺ 438, found 438. SFC 99.93% de.

Examples 65 & 66: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(K8) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(K9)

Synthesis of K1

To a solution of 2,6-di-tert-butyl-4-methylphenol (24 g, 108 mmol) intoluene (30 mL) under nitrogen at 0° C. was added AlMe₃ (2 M in toluene,27 mL, 54 mmol) dropwise. The mixture was stirred at 25° C. for 1 h andused directly as a solution of MAD. To the MAD (54 mmol in 30 mLtoluene) solution was added a solution of A24 (5 g, 18.2 mmol) intoluene (20 mL) dropwise at −60° C. After stirring at −60° C. for 1 hunder N₂, n-prMgBr (27.3 mL, 54.6 mmol, 2M in THF) was added drop wiseat −60° C. After stirring at −60° C. for another 4 h, the reactionmixture was poured into saturated aqueous citric acid (100 mL) below 10°C. and extracted with EtOAc (2×100 mL). The combined organic solutionwas dried over Na₂SO₄, filtered and concentrated in vacuum. The residuewas purified by a silica gel column (PE/EtOAc=0-20%) to give K1 (3.83 g,66.1%) as a solid.

¹H NMR (400 MHz, CDCl3) δ_(H) 2.49-2.37 (m, 1H), 2.31-1.98 (m, 2H),1.97-1.87 (m, 1H), 1.86-1.73 (m, 4H), 1.72-1.60 (m, 2H), 1.55-1.45 (m,5H), 1.45-1.27 (m, 10H), 1.27-1.00 (m, 4H), 0.93 (t, J=7.2 Hz, 3H), 0.87(s, 3H).

Synthesis of K2

To a mixture of EtPPh₃Br (26.5 g, 71.4 mmol) in THF (50 mL) was addedt-BuOK (8.01 g, 71.4 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 min. K1 (3.8 g, 11.9 mmol) was added in portions below 40° C.After stirring at 40° C. for 1 h, the reaction mixture was quenched with10% NH₄Cl aqueous (100 mL) at 15° C. and extracted with EtOAc (500 mL).The combined organic solution was concentrated under vacuum and waspurified by trituration with MeOH/H₂O (1:1, 300 mL) at reflux to give K2(4.5) as an oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 5.10 (d, J=7.2 Hz, 1H), 2.41-2.09 (m, 4H),1.78-1.71 (m, 3H), 1.66-1.63 (m, 3H), 1.56-1.51 (m, 3H), 1.50-1.42 (m,3H), 1.37-1.29 (m, 6H), 1.21-1.00 (m, 6H), 0.93 (t, J=7.28 Hz, 3H), 0.87(s, 3H).

Synthesis of K3

To a solution of K2 (4.5 g, 13.6 mmol) in THF (50 mL) was added 9-BBNdimer (9.95 g, 40.8 mmol) at 15° C. After stirring at 40° C. for 1 h,ethanol (6.21 g, 135 mmol) was added at 15° C. followed by aqueous NaOH(27 mL, 5M, 135 mmol) at −10° C. and finally H₂O₂ (13.5 mL, 10 M, 135mmol) dropwise. After stirring at 80° C. for 1 h, the reaction wasquenched with sat. Na₂S₂O₃ (50 mL). After stirring for 30 min, themixture was extracted with EtOAc (100 mL). The combined organic solutionwas washed with saturated brine (2×100 mL), dried over anhydrous Na₂SO₄,concentrated under vacuum and purified by silica gel chromatography(PE/EtOAc=10 to 20%) to give K3 (3.2 g, 67.5%) as a solid.

¹H NMR (400 MHz, CDCl3) δ_(H) 3.74-3.66 (m, 1H), 1.85-1.60 (m, 10H),1.49-1.29 (m, 13H), 1.22 (d, J=6 Hz, 3H), 1.16-1.00 (m, 7H), 0.93 (t,J=7.2 Hz, 3H), 0.66 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₃H₄₀O₂[M+H-2H₂O]⁺313.3, found 313.3.

Synthesis of K4

To a solution of K3 (3.1 g, 8.89 mmol) in DCM (30 mL) was addedDess-martin periodane (7.5 g, 17.7 mmol) at 25° C. After stirring for 10min, the mixture was quenched by saturated NaHCO₃/Na₂S₂O₃ aqueous (1:1,375 mL) at 25° C. The organic solution was separated and washed withsaturated NaHCO₃/Na₂S₂O₃ aqueous (1:1, 375 mL), brine (200 mL), driedover Na₂SO₄, filtered and concentrated under vacuum, to give K4 (4 g) asa solid.

¹H NMR (400 MHz, CDCl3) δ_(H) 2.40 (d, J=12.80 Hz, 1H), 2.11 (s, 3H),1.93-1.81 (m, 4H), 1.72-1.63 (m, 8H), 1.50-1.41 (m, 8H), 1.13-1.02 (m,6H), 0.94-0.91 (m, 3H), 0.62 (s, 3H).

Synthesis of K5

To a mixture of MePPh₃Br (12.3 g, 34.5 mmol) in THF (50 mL) was addedt-BuOK (3.87 g, 34.5 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 min, K4 (4 g, 11.5 mmol) was added in portions below 50° C. Afterstirring at 50° C. for 1 h, the reaction mixture was quenched with 10%NH4Cl aqueous (100 mL) at 15° C. and extracted with EtOAc (200 mL). Thecombined organic solution was concentrated under vacuum and purified bysilica gel chromatography (PE/EtOAc=0 to 5%) to give K5 (600 mg, 15.1%)as a solid.

¹H NMR (400 MHz, CDCl3) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H) 2.04-1.99 (m,2H), 1.86-1.76 (m, 3H), 1.75 (s, 3H), 1.74-1.57 (m, 6H), 1.56-1.50 (m,2H), 1.49-1.28 (m, 10H), 1.23-0.97 (m, 6H), 0.93 (t, J=7.2 Hz, 3H), 0.56(s, 3H).

Synthesis of K6

To a solution of K5 (600 mg, 1.74 mmol) in THF (5 mL) was added BH₃.Me₂S(0.87 mL, 8.7 mmol, 10 M) dropwise at 0° C. After stirring at 25° C. for3 h, the reaction mixture was cooled to 0° C. and ethanol (800 mg, 17.4mmol) followed by NaOH aqueous (1.73 mL, 17.4 mmol, 5 M) and finallyH₂O₂ (1.73 mL, 17.4 mmol) were added. After stirring at 70° C. for 1 h,the mixture was extracted with EtOAc (2×50 mL). The combined organicsolution was washed with saturated Na₂S₂O₃ aqueous (2×20 mL), brine (50mL), dried over Na₂SO₄, filtered and evaporated to give K6 (620 mg) asan oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 3.85-3.58 (m, 1H), 3.52-3.29 (m, 1H),2.01-1.71 (m, 5H), 1.71-1.58 (m, 4H), 1.51-1.12 (m, 17H), 1.11-0.98 (m,6H), 0.97-0.90 (m, 5H), 0.68 (s, 3H).

Synthesis of K7

To a solution of K6 (620 mg, 1.7 mmol) in DCM (10 mL) at 0° C. was addedPPh₃ (668 mg, 2.55 mmol) and NBS (453 mg, 2.55 mmol). After stirring at25° C. for 2 h, the reaction was diluted with water (50 mL) andextracted with DCM (2×80 mL). The combined organic solution was washedwith saturated brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give K7 (385 mg, 67.5%) as an oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 3.67-3.45 (m, 1H), 3.34 (s, 1H), 1.97-1.72(m, 5H), 1.72-1.50 (m, 7H), 1.49-1.28 (m, 12H), 1.22-1.16 (m, 1H),1.15-1.03 (m, 5H), 1.00 (d, J=6.4 Hz, 3H), 0.92 (t, J=7.2 Hz, 3H), 0.68(s, 3H).

Synthesis of K8 & K9

To a solution of K7 (200 mg, 0.47 mmol) in acetone (5 mL) were added1H-pyrazole-4-carbonitrile (52.5 mg, 0.56 mmol) and K₂CO₃ (129 mg, 0.94mmol). After stirring at 25° C. for 14 h, the mixture was diluted withwater (40 mL) and extracted with EtOAc (2×50 mL). The combined organicsolution was dried over Na₂SO₄, filtered, concentrated and purified byflash column (20˜50% of EtOAc in PE) to give a mixture of K8 & K9 (100mg) as a solid. The diastereomers were separated by SFC (Column: DAICELCHIRALCEL OD-H (250 mm*30 mm, 5 um), Condition: 0.1% NH3H2O EtOH, BeginB: 30%, End B: 30%, FlowRate(ml/min): 50) to afford K8 (43 mg, 43%) andK9 (30 mg, 30%) as solids. The C20-Me diastereomers were assigned basedon ¹H NMR of C21-Me.

K8: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.75 (s, 1H), 7.80 (s, 1H), 4.50 (dd,J=13.2, 4.39 Hz, 1H), 3.66 (dd, J=13.2, 10.00 Hz, 1H), 2.18-2.02 (m,1H), 1.87-1.76 (m, 3H), 1.68-1.59 (m, 3H), 1.50-1.41 (m, 3H), 1.41-1.18(m, 14H), 1.18-1.00 (m, 6H), 0.93 (t, J=7.2 Hz, 3H), 0.79 (s, 3H), 0.68(d, J=6.4 Hz, 3H).

LC-ELSD/MS 30-90AB_2 min_E, purity 99%, 100% de based on H-NMR; MS ESIcalcd. for C₂₈H₄₃N₃O [M+H—H₂O]⁺420.3, found 420.3.

K9: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.19-4.32(m, 1H), 3.72 (dd, J=13.13, 9.2 Hz, 1H), 1.87-2.08 (m, 3H), 1.59-1.84(m, 6H), 1.22-1.51 (m, 14H), 1.00-1.20 (m, 7H), 0.93 (t, J=7.2 Hz, 3H),0.81 (d, J=6.4 Hz, 3H), 0.71 (s, 3H).

LC-ELSD/MS 30-90AB_2 min_E, purity 99%, purity 99%, 100% de based onH-NMR; MS ESI calcd. for C₂₈H₄₃N₃O [M+H—H₂O]⁺420.3, found 420.3.

Examples 67 & 68: Synthesis of4-cyano-N—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)benzenesulfonamide(67) &4-cyano-N—((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-N-methylbenzenesulfonamide(68)

Synthesis of 67

To a solution of F7 (300 mg, 0.825 mmol) in DCM (5 mL) was added Et₃N(208 mg, 2.06 mmol) and 4-cyanobenzene-1-sulfonyl chloride (247 mg, 1.23mmol) at 25° C. After stirring at 25° C. for 16 h, the resultingcolorless solution was washed with water (3×100 mL). The organic layerwas dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (30% of EtOAc in PE) to give 67 (398 mg) asa solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.00-7.98 (m, 2H), 7.81-7.79 (m, 2H), 4.27(d, J=8.8 Hz, 1H), 3.53 (q, J=6.8 Hz, 2H), 3.45-3.38 (m, 3H), 2.73 (s,1H), 1.99-1.96 (m, 1H), 1.82-1.66 (m, 4H), 1.63-1.50 (m, 7H), 1.47-1.13(m, 10H), 1.11-0.83 (m, 8H), 0.56 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₃₀H₄₄N₂O₄SNa [M+Na]⁺551, found 551.

Synthesis of 68

To a solution of 67 (150 mg, 0.283 mmol) in DMF (3 mL) was added Cs₂CO₃(184 mg, 0.566 mmol). After stirring for 20 mins at 25° C., Mel (60.1mg, 0.424 mmol) was added to the reaction mixture. After stirring at 25°C. for 16 h, the resulting mixture was poured into water (50 mL) andextracted with EtOAc (2×50 mL). The combined organic layer was washedwith water (3×100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by pre-HPLC (column: Xtimate C18150*25 mm*5 um, condition: water (0.225% FA)-ACN, Begin B: 77, End B:100) to give 68 (89 mg, 58%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.94-7.92 (m, 2H), 7.81-7.79 (m, 2H),4.07-3.99 (m, 1H), 3.53 (q, J=6.8 Hz, 2H), 3.46 (q, J=9.2 Hz, 2H),2.73-2.66 (m, 4H), 2.10-2.07 (m, 1H), 1.85-1.47 (m, 10H), 1.44-1.18 (m,10H), 1.14-0.99 (m, 6H), 0.86 (s, 3H), 0.64 (d, J=6.4 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₁H₄₅N₂O₃S [M+H—H₂O]⁺525,found 525.

The following examples were synthesized similar to Examples 67 & 68 withthe listed sulfonyl chloride or alkylating agent and an appropriate SM.

Sulfonyl chloride/ alkylating Ex SM agent STRUCTURE Analytical 69 F8 3-cyanobenzene- 1-sulfonyl chloride

1H NMR (400 MHz, CDCl₃) δ_(H) 8.17 (s, 1H), 8.11 (d, J = 8 Hz, 1H), 7.84(d, J = 8 Hz, 1H), 7.65 (t, J = 8 Hz, 1H), 4.28 (d, J = 8.8 Hz, 1H),3.53 (q, J = 6.8 Hz, 2H), 3.43 (q, J = 9.2 Hz, 3H), 2.73 (s, 1H),2.00-1.97 (m, 1H), 1.84-1.65 (m, 3H), 1.63- 1.50 (m, 7H), 1.47-1.30 (m,5H), 1.29-1.12 (m, 5H), 1.09-0.87 (m, 9H), 0.57 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₃₀H₄₄N₂O₄SNa [M + Na]⁺ 551, found 551. 7069 MeI

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.11 (s, 1H), 8.05 (d, J = 8 Hz, 1H), 7.85(d, J = 8 Hz, 1H), 7.65 (t, J = 8 Hz, 1H), 4.07-4.00 (m, 1H), 3.53 (q, J= 6.8 Hz, 2H), 3.43 (q, J = 9.2 Hz, 3H), 2.71-2.66 (m, 4H), 2.10- 2.05(m, 1H), 1.84-1.47 (m, 9H), 1.44-1.18 (m, 10H), 1.14-1.00 (m, 6H), 0.86(s, 3H), 0.65 (d, J = 6.8 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₃₁H₄₅N₂O₃S [M + H − H₂O]⁺ 525, found 525. 71 F8 6- cyanopyridine-2- sulfonyl chloride

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.23- 8.19 (m, 1H), 8.10-8.05 (m, 1H),7.87-7.83 (m, 1H), 4.63 (d, J = 9.2 Hz, 1H), 3.53 (q, J = 7.2 Hz, 3H),3.47-3.39 (m, 2H), 2.23-2.16 (m, 1H) 1.87-1.57 (m, 9H), 1.49-1.30 (m,8H), 1.21 (t, J = 6.8 Hz, 4H), 1.16-0.98 (m, 6H), 0.93 (d, J = 6.4 Hz,3H), 0.69 (s, 3H). LC-ELSD purity 99%, MS ESI calcd. for C₂₉H₄₂N₃O₄S [M− H]⁺ 528, found 528. 72 71 MeI

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.20- 8.17 (m, 1H), 8.09-8.04 (m, 1H),7.85-7.82 (m, 1H), 4.13-4.05 (m, 1H), 3.56-3.50 (m, 2H), 3.47-3.39 (m,2H), 2.84 (s, 3H), 2.73-2.67 (m, 1H), 2.15-2.08 (m, 1H), 1.87-1.74 (m,3H), 1.68-1.56 (m, 4H), 1.49- 1.25 (m, 9H), 1.21 (t, J = 6.8 Hz, 4H),1.16-1.04 (m, 6H), 0.88-0.86 (m, 3H), 0.85 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₃₀H₄₅N₃O₄SNa [M + Na]⁺ 566, found 566. 73 F8 5-cyanopyridine-2- sulfonyl chloride

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.96 (dd, J = 0.8, 2.0 Hz, 1H), 8.22- 8.17(m, 1H), 8.16-8.10 (m, 1H), 4.62 (d, J = 9.2 Hz, 1H), 3.53 (q, J = 6.8Hz, 3H), 3.49-3.36 (m, 2H), 2.73 (br s, 1H), 2.22-2.06 (m, 1H), 1.90-1.57 (m, 7H), 1.36 (br s, 8H), 1.21 (t, J = 7.2 Hz, 11H), 0.87 (d, J =6.4 Hz, 3H), 0.65-0.65 (m, 1H), 0.69 (s, 2H). LC-ELSD/MS purity 99%, MSESI calcd. for C₂₉H₄₂N₃O₃₅ [M − H₂O + H]⁺ 512.3 found 512.3. 74 73 MeI

¹H NMR (400 MHz, CDCl₃) δH 8.95 (s, 1H), 8.26-7.96 (m, 2H), 4.15-4.02(m, 1H), 3.53 (q, J = 7.2 Hz, 2H), 3.48-3.37 (m, 2H), 2.83 (s, 3H), 2.71(br s, 1H), 2.10 (br d, J = 11.6 Hz, 1H), 1.90-1.50 (m, 7H), 1.50-1.15(m, 12H), 1.15-1.00 (m, 7H), 0.85 (s, 3H), 0.79 (br d, J = 6.8 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₄N₃O₃S [M − H₂O + H]⁺526.3 found 526.3. 75 E2 6- cyanopyridine-2- sulfonyl chloride

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.23 − 8.19 (m, 1H), 8.11 −8.05 (m, 1H),7.87 −7.83 (m, 1H), 4.68 −4.62 (m, 1H), 3.58 −3.47 (m, 1H), 3.39 (s,5H), 2.64 −2.56 (m, 1H), 2.23 −2.15 (m, 1H), 1.87- 1.80 (m, 1H), 1.78-1.59 (m, 5H), 1.48 −1.31 (m, 8H), 1.27 −1.02 (m, 9H), 0.92 (d, J = 6.4Hz, 3H), 0.69 (s, 3H). LC-ELSD purity 99%, MS ESI calcd. for C₂₈H₄₀N₃O₄S[M − H]⁺ 514, found 514.

Example 76: Synthesis of2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-N-phenylacetamide(76)

Synthesis of 76.1

To a solution of bromo (methoxymethyl) triphenyl-λ⁵-phosphane (3.81 g,9.8 mmol) in THF (20 mL) was added tert-butyllithium (7.5 mL, 9.8 mmol,2.5 M in n-hexane) at 0° C. After stirring at 0° C. for 1 h, A3 (1 g,3.3 mmol) in THF (10 mL) was added at 0° C. After stirred at 15° C. for1 h, the mixture was treated with NH₄Cl (20 mL, 10%) and extracted withEtOAc (2×40 mL). The combined organic phase was washed with brine (40mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜ 13% of EtOAc in PE) to give 76.1 (840mg, 77.0%) as an oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 6.22 (d, J=12.4 Hz, 0.6H), 5.93 (d, J=6.4Hz, 0.4H), 4.67-4.60 (m, 0.6H), 4.27-4.22 (m, 0.4H), 3.53 (d, J=10.0 Hz,3H), 1.80 (br s, 5H), 1.68-1.59 (m, 4H), 1.49-1.21 (m, 16H), 1.19-0.91(m, 7H), 0.59 (d, J=5.2 Hz, 3H).

Synthesis of 76.2

To a solution 76.1 (840 g, 2.5 mmol) in acetone (15 mL) was added TsOH(6.50 g, 37.8 mmol) at 15° C. After stirring at 15° C. for 10 mins, themixture was poured into water (20 mL) and extracted with DCM (2×40 mL).The combined organic phase was washed with NaHCO₃ (20 mL), brine (20mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜ 15% of EtOAc in PE) to give 76.2 (550mg, 27.6%) as a solid

¹H NMR (400 MHz, CDCl3) δ_(H) 9.76 (t, J=2.4 Hz, 1H), 2.52-2.44 (m, 1H),2.28-2.18 (m, 1H), 1.99-1.76 (m, 5H), 1.70-1.60 (m, 4H), 1.50-1.36 (m,6H), 1.33-1.23 (m, 8H), 1.17-1.01 (m, 6H), 0.93-0.78 (m, 2H), 0.60 (s,3H).

Synthesis of 76.3

To a solution of 76.2 (550 mg, 1.7 mmol) in acetone (7.5 mL) and2-methyl-2-butene (2 mL) was added dropwise aqueous NaH₂PO₄ (1.03 g, 8.6mmol) and NaClO₂ (777 mg, 8.6 mmol) in water (5 mL) at 0° C. Afterstirring at 15° C. for 2 h, the resulting colorless solution was pouredinto water (20 mL) and filtered. The filter cake was washed with water(50 mL) and concentrated to afford 76.3 (390 mg, 67.8%) as a solid.

¹H NMR (400 MHz, CDCl3) δ_(H) 2.45-2.34 (m, 1H), 2.17-2.08 (m, 1H),2.00-1.75 (m, 5H), 1.71-1.58 (m, 4H), 1.26 (s, 19H), 0.60 (s, 3H).

Synthesis of 76

To a solution of 76.3 (150 mg, 0.45 mmol) in DCM (3 mL) was added HATU(340 mg, 0.9 mmol), TEA (225 mg, 2.2 mmol) and aniline (83.4 mg, 0.9mmol) at 25° C. After stirring at 25° C. for 16 h, the mixture waspoured into water (15 mL), the aqueous phase was extracted with EtOAc(2×30 mL). The combined organic phase was washed with brine (20 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue wastriturated from MeCN (5 mL) at 15° C., then washed with HCl (5 ml) andNaHCO₃ (5 ml) to give 76 (130 mg, 71.0%) as a solid.

¹H NMR (400 MHz, CDCl3) δ_(H) 7.50 (br d, J=8.0 Hz, 2H), 7.32 (t, J=7.6Hz, 2H), 7.13-7.06 (m, 2H), 2.45 (dd, J=4.8, 14.0 Hz, 1H), 2.17-2.09 (m,1H), 2.05-1.72 (m, 6H), 1.70-1.63 (m, 3H), 1.50-1.24 (m, 14H), 1.18-1.00(m, 6H), 0.64 (s, 3H). LCMS Rt=1.189 min in 2 min chromatography,30-90AB_2 min_E.M (Mobile Phase: 1.5 mL/4 LTFA in water (solvent A) and0.75 mL/4 LTFA in acetonitrile (solvent B), using the elution gradient30%-90% (solvent B) over 0.9 minutes and holding at 90% for 0.6 minutesat a flow rate of 1.2 mL/min; Column: Xtimate C18 2.1*30 mm, 3 □m;Wavelength: UV 220 nm; Column temperature: 50° C.; MS ionization: ESI;Detector: PDA&ELSD), purity 99%, MS ESI calcd for C₂₇H₄₀NO₂ [M+H]⁺ 410,found 410.

Examples 77 & 78: Synthesis of5-cyano-N—((R)-1-((3R,5R,8R,9S,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-N-methylpyridine-2-sulfonamide(77) &5-cyano-N—((R)-1-((3R,5R,8R,9S,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)pyridine-2-sulfonamide(78)

Synthesis of 77.1

To a solution of 207.1 (3.6 g, 9.92 mmol) and (1R)-1-phenylethan-1-amine(7.21 g, 59.4 mmol) in DCE (40 mL) at 25° C. under N₂, then NaCNBH₃(4.91 g, 79.3 mmol) was added. After stirring at 50° C. for 16 h, thereaction was quenched with water (50 mL), extracted with DCM (2×50 mL).The combined organic phase was washed with 10% HCl (2×100 mL), saturatedNaHCO₃ (100 mL), brine (100 mL), dried over Na₂SO₄, filtered andconcentrated to give 77.1 (5 g) as oil.

Synthesis of 77.2

To a solution of 77.1 (2.5 g, 5.18 mmol) in EtOH (50 mL) was addedPd—C(dry, 500 mg) and one drop NH₃H₂O. The mixture was stirred under H₂(50 psi) at 50° C. for 48 h to give a suspension. The reaction mixturewas filtered through a pad of Celite and washed with EtOH (3×50 mL). Thefiltrate was concentrated. The residue was purified by flash column(0˜5% of MeOH in DCM) to give 77.2 (1.3 g, 66.6%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.53 (q, J=7.0 Hz, 2H), 3.46-3.35 (m, 2H),2.90-2.76 (m, 1H), 2.01-1.64 (m, 6H), 1.61-1.34 (m, 11H), 1.32-0.97 (m,15H), 0.93 (s, 3H), 0.70 (s, 3H).

Synthesis of 77

To a solution of 77.2 (300 mg, 0.794 mmol) and5-cyanopyridine-2-sulfonyl chloride (320 mg, 1.58 mmol) in DCM (10 mL)was added TEA (801 mg, 7.94 mmol) at 15° C. to give a solution. After 15mins, the mixture was poured into water (30 mL), stirred for 20 mins,and then extracted with DCM (3×20 mL). The combined organic phase waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜20% of EtOAcin PE) to give 77 (58 mg, 13.4%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.96 (s, 1H), 8.22-8.16 (m, 1H), 8.15-8.10(m, 1H), 4.63 (d, J=9.1 Hz, 1H), 3.59-3.48 (m, 3H), 3.47-3.36 (m, 2H),2.74 (s, 1H), 2.17 (br d, J=11.4 Hz, 1H), 1.97-1.59 (m, 5H), 1.51-1.28(m, 8H), 1.25-0.97 (m, 12H), 0.93 (s, 3H), 0.86 (d, J=6.3 Hz, 3H), 0.68(s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₄N₃O₃S[M+H—H₂O]⁺526.3 found 526.3.

Synthesis of 78

To a solution of 77 (100 mg, 0.184 mmol) in DMF (3 mL) was added Mel(65.2 mg, 0.459 mmol) and Cs₂CO₃ (120 mg, 0.367 mmol). After stirring at25° C. for 16 h, the mixture was poured into water (20 mL) and extractedwith EtOAc (2×20 mL). The combined organic phase was washed with brine(20 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜20% of EtOAc in PE) to give 78(22.2 mg, 21.7%) as a solid. ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.95 (s, 1H),8.21-8.14 (m, 1H), 8.12-8.03 (m, 1H), 4.23-3.91 (m, 1H), 3.53 (q, J=6.9Hz, 2H), 3.48-3.33 (m, 2H), 2.82 (s, 3H), 2.72 (s, 1H), 2.11 (br d,J=12.3 Hz, 1H), 1.99-1.78 (m, 2H), 1.76-1.58 (m, 4H), 1.38 (br d, J=13.1Hz, 8H), 1.21 (br t, J=6.9 Hz, 9H), 0.94 (s, 3H), 0.84 (s, 3H), 0.79 (d,J=6.5 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₁H₄₆N₃O₃S[M+H—H₂O]⁺540.3 found 540.3.

Examples 87 & 88: Synthesis of1-(2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-3-carbonitrile(87) &1-(2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-5-carbonitrile(88)

Synthesis of 87.2

To a solution of NaH (8.00 g, 60%, 200 mmol) in DMSO (100 mL) was addeda solution of trimethylsulfonium iodide (40.7 g, 200 mmol) in THF (100mL) dropwise at 0° C. over 30 mins under N₂. The resulting mixture wasadded into a solution of 87.1 (50 g, 182 mmol) in DMSO (100 mL). Afterstirring at 25° C. for 12 h, the resulting suspension was poured intoice-water (v/v=1/1) (400 mL), stirred for 20 mins and extracted withEtOAc (3×400 mL). The combined organic phase was washed with brine(2×200 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was triturated from MeOH (300 mL) at 25° C. to give 87.2 (45 g)as a solid.

¹H NMR (400 MHz, CDCl₃) δ 2.67-2.37 (m, 2H), 2.28-2.13 (m, 2H),2.12-2.04 (m, 1H), 1.99-1.89 (m, 1H), 1.88-1.72 (m, 4H), 1.70-1.60 (m,2H), 1.58-1.43 (m, 5H), 1.41-1.04 (m, 8H), 0.92-0.82 (m, 3H)

Synthesis of 87.3

Na (21.5 g, 935 mmol) was added into MeOH (250 mL) at 25° C. inportions. After stirring at 25° C. for 2 h under N₂, 87.2 (45 g, 156mmol) in MeOH (150 mL) was added. After stirring at 75° C. for 12 h, theresulting solution was cooled to 25° C. and poured into water (400 mL).The aqueous phase was extracted with EtOAc (3×400 mL). The combinedorganic phase was washed with brine (2×200 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜15% of EtOAc in PE) to give 87.3 (30 g) as an oil and C3epimer (12 g, 24.0%) as a solid. 87.3 was re-purified by flash column[0˜5% of EtOAc in PE and DCM (1:1)] to give 87.3 (9 g, 30%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ 3.47-3.31 (m, 5H), 2.42 (dd, J=8.4, 19.2 Hz,1H), 2.12-2.02 (m, 1H), 1.96-1.89 (m, 1H), 1.86-1.67 (m, 6H), 1.60-1.41(m, 7H), 1.38-1.17 (m, 7H), 1.10-1.00 (m, 1H), 0.85 (s, 3H).

Synthesis of 87.4

To a stirred solution of NaH (2.05 g, 51.4 mmol, 60% in oil) in THF (40mL) were added ethyl 2-(diethoxyphosphanyl (8.73 g, 39.0 mmol) at 25° C.for 10 mins under N₂ followed by 87.3 (5 g, 15.6 mmol). After stirringat 65° C. for 12 h, the mixture was cooled to room temperature andconcentrated. The residue was poured into ice-water (v/v=1/1) (50 mL),stirred for 20 mins and extracted with EtOAc (3×100 mL). The combinedorganic phase was washed with brine (2×100 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜50% of EtOAc in PE) to give 87.4 (280 mg, 4.6%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.52 (t, J=2.4 Hz, 1H), 4.20-4.09 (m, 2H),3.46-3.35 (m, 6H), 2.82 (dt, J=3.1, 6.0 Hz, 2H), 2.67-2.54 (m, 1H),1.89-1.73 (m, 7H), 1.66-1.56 (m, 5H), 1.36-1.22 (m, 9H), 0.98-0.84 (m,2H), 0.81 (s, 3H).

Synthesis of 87.5

To a solution of 87.4 (280 mg, 0.71 mmol) in EtOH (10 mL) was added Pd/C(wet, 10%, 600 mg) under N₂. The suspension was degassed under vacuumand purged with H₂ for three times. After stirring under H₂ (15 psi) at25° C. for 12 h, the resulting suspension was filtered through a pad ofCelite and washed with EtOH (3×10 mL). The filtrate was concentrated togive 87.5 (250 mg, 96%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.11 (q, J=6.8 Hz, 2H), 3.72 (q, J=6.8 Hz,2H), 3.42-3.37 (m, 5H), 2.35 (dd, J=4.8, 14.4 Hz, 1H), 2.09 (dd, J=9.8,14.4 Hz, 1H), 1.83-1.74 (m, 4H), 1.69-1.56 (m, 5H), 1.45-1.34 (m, 6H),1.27-1.22 (m, 6H), 1.12-1.04 (m, 4H), 0.59 (s, 3H).

Synthesis of 87.6

To a solution of 87.5 (250 mg, 0.63 mmol) in THF (5 mL) was added LiAlH₄(36.2 mg, 0.95 mmol) in one portion at 20° C. under N₂. After stirringat 20° C. for 12 h, H₂O (2 ml) was added to the resulting graysuspension and the mixture was acidified with 1 M HCl to pH ˜5. Theaqueous phase was extracted with EtOAc (3×10 mL). The combined organicphase was washed with brine (2×20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜50% of EtOAc in PE) to give 87.6 (200 mg, 90%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.72 (d, J=3.2 Hz, 1H), 4.68 (d, J=2.4 Hz,1H), 3.72-3.57 (m, 2H), 3.46-3.33 (m, 6H), 2.76-2.44 (m, 1H), 1.93-1.74(m, 5H), 1.69-1.52 (m, 7H), 1.46-1.33 (m, 7H), 1.16-1.03 (m, 5H), 0.59(s, 3H).

Synthesis of 87.7

To a solution of 87.6 (200 mg, 0.57 mmol) in DCM (5 mL) at 0° C. wasadded PPh₃ (179 mg, 0.68 mmol) and NBS (116 mg, 0.68 mmol). Afterstirring at 20° C. for 12 h, the resulting solution was poured intowater (20 mL) and extracted with EtOAc (3×30 mL). The combined organicphase was washed with brine (2×20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜15% of EtOAc in PE) to give 87.7 (120 mg, 51%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.49-3.29 (m, 6H), 2.58 (s, 1H), 1.96-1.75(m, 4H), 1.60-1.50 (m, 9H), 1.45-1.04 (m, 14H), 0.59 (s, 3H).

Synthesis of 87 & 88

To a solution of 87.7 (120 mg, 0.2902 mmol) in DMF (5 mL) were addedCs₂CO₃ (190 mg, 0.5804 mmol) and 1H-pyrazole-3-carbonitrile (54 mg,0.5804 mmol) at 25° C. After stirring at 85° C. for 12 h, the resultingmixture was cooled to 25° C., poured into water (10 mL). and extractedwith EtOAc (3×10 mL). The combined organic phase was washed with brine(2×20 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜ 50% of EtOAc in PE) to give 87(48.4 mg, 39%) as a solid and 88, which was further purified by SFC(Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, Sum), Flow Rate(mL/min):40) to afford 87 (4.1 mg, 3.3%) as a solid.

87: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.44 (d, J=2.4 Hz, 1H), 6.65 (d, J=2.4Hz, 1H), 4.23-4.07 (m, 2H), 3.43-3.34 (m, 5H), 2.59 (brs, 1H), 2.04-1.94(m, 1H), 1.88-1.73 (m, 4H), 1.67-1.56 (m, 9H), 1.50-1.35 (m, 5H),1.28-1.18 (m, 3H), 1.09-0.96 (m, 4H), 0.60 (s, 3H). LC-ELS/MS purity99%, MS ESI calcd. for C₂₆H₃₉N₃O₂Na [M+Na]⁺448.3, found 448.3.

88: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.56 (d, J=2.4 Hz, 1H), 6.77 (d, J=2.0Hz, 1H), 4.37-4.21 (m, 2H), 3.46-3.34 (m, 5H), 2.57 (brs, 1H), 2.15-2.01(m, 1H), 1.92-1.73 (m, 4H), 1.72-1.61 (m, 6H), 1.49-1.36 (m, 5H),1.30-1.17 (m, 4H), 1.15-0.94 (m, 6H), 0.60 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₆H₄₀N₃O₂ [M+H]⁺ 426.3, found 426.3.

Example 89: 1-(2-((3R, 5R, 8R, 9R, 10S, 13R, 145, 17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile

Synthesis of 89.1

To a suspension of NaH (2.75 g, 60%, 68.8 mmol) in THF (60 mL) was added(EtO)₂P(O)CH₂COOEt (15.4 g, 68.8 mmol) dropwise at 0° C. After stirringat 20° C. for 10 min, a solution of 89.0 (10 g, 34.4 mmol) in THF (20mL) was added dropwise at 20° C. After stirring at 70° C. for 16 h, thereaction mixture was poured into NH₄Cl (200 mL, 10% aq) and extractedwith EtOAc (200 mL). The organic layer was separated, dried over Na₂SO₄,filtered, concentrated. The residue was purified by flash column (0˜20%EtOAc in PE) to give 89.1 (12 g, 97%) as an oil. ¹H NMR (400 MHz, CDCl₃)δ_(H) 5.52 (t, J=2.4 Hz, 1H), 4.15 (q, J=7.2 Hz, 2H), 2.90-2.75 (m, 2H),1.95-1.60 (m, 5H), 1.50-1.25 (m, 18H), 1.20-1.05 (m, 4H), 0.82 (s, 3H).

Synthesis of 89.2

To a solution of 89.1 (12 g, 33.2 mmol) in THF (150 mL) was added Pd/C(2 g, dry, 10%) under N₂. After stirring under H₂ (40 psi) at 40° C. for24 h, the reaction mixture was filtered through a pad of celite whichwas then washed with THF (3×50 mL). The combined filtrate wasconcentrated to give 89.2 (11.7 g, 97.5%) as an oil. ¹H NMR (400 MHz,CDCl₃) δ_(H) 4.11 (q, J=6.8 Hz, 2H), 2.35 (dd, J=5.2, 14.4 Hz, 1H), 2.10(dd, J=10.0, 14.8 Hz, 1H), 2.00-1.75 (m, 6H), 1.70-1.50 (m, 3H),1.50-1.35 (m, 6H), 1.35-1.25 (m, 10H), 1.20-0.95 (m, 6H), 0.59 (s, 3H).

Synthesis of 89.3

To a suspension of LiAlH₄ (6.0 g, 158 mmol) in THF (120 mL) was added asolution of 89.2 (11.1 g, 30.6 mmol) in THF (30 mL) at 0° C. under N₂.After stirring at 0° C. for 10 min, to the mixture was added water/THF(6 mL/200 mL) dropwise followed by NaOH (6 mL, 10% aq.) and water (18mL). The mixture was filtered and the precipitate was washed with THF(3×100 mL). The combined filtrate was concentrated and triturated in DCM(50 mL) to give 89.3 (9 g, 92%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.55 (m, 2H), 1.90-1.60 (m, 9H),1.50-1.15 (m, 16H) 1.15-0.90 (m, 6H), 0.59 (s, 3H).

Synthesis of 89.4

To a solution of 89.3 (300 mg, 0.935 mmol) in DCM (8 mL) at 0° C. wasadded PPh₃ (487 mg, 1.86 mmol) and NBS (331 mg, 1.86 mmol). Afterstirring at 25° C. for 3 h to give a solution, the mixture was pouredinto water (20 mL) and extracted with DCM (3×20 mL). The combinedorganic phase was washed with brine (2×50 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜30% of EtOAc in PE) to give 89.4 (310 mg) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.49-3.41 (m, 1H), 3.37-3.27 (m, 1H),2.02-1.91 (m, 1H), 1.89-1.78 (m, 4H), 1.74-1.61 (m, 6H), 1.49-1.35 (m,7H), 1.34-1.28 (m, 2H), 1.26 (s, 3H), 1.23-1.18 (m, 1H), 1.15-1.02 (m,6H), 0.59 (s, 3H).

Synthesis of 90

To a solution of 89.4 (310 mg, 0.808 mmol) in DMF (5 mL) were added1H-pyrazole-4-carbonitrile (222 mg, 1.61 mmol) and K₂CO₃ (75.2 mg, 0.808mmol). After stirring at 50° C. for 16 h, the mixture was treated withwater (20 mL) and extracted with EtOAc (2×20 mL). The combined organicphase was washed with brine (20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜30% of EtOAc in PE) to give 90 (200 mg, 62.6%) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.78 (s, 1H), 4.22-4.04 (m,2H), 2.05-1.97 (m, 1H), 1.89-1.76 (m, 4H), 1.72-1.59 (m, 6H), 1.50-1.28(m, 8H), 1.26 (s, 5H), 1.23-1.20 (m, 1H), 1.09-0.96 (m, 5H), 0.60 (s,3H). LC-ELSD/MS purity>99%, MS ESI calcd. for C₂₅H₃₇N₃O [M−H₂O+H]⁺378.3,found 378.3.

Example 90: Synthesis of6-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)amino)nicotinonitrile(90)

Synthesis of 90.0

To a solution of 2,6-di-tert-butyl-4-methylphenol (13.1 g, 59.6 mmol) intoluene (20 mL) was added AlMe₃ (14.9 mL, 29.8 mmol, 2 M in toluene)dropwise at 0° C. After stirring at 25° C. for 30 min. To the MADsolution was added a solution of 271.2 (3 g, 9.91 mmol) in anhydroustoluene (40 mL) dropwise at −70° C. After stirring at −70° C. for 1 hunder N₂, n-PrMgCl (14.8 mL, 29.7 mmol, 2 M in diethyl ether) was addeddrop wise at −70° C. The resulting solution was stirred at −70° C. foranother 2 h to give a solution. The reaction mixture was poured intosaturated aqueous citric acid (100 mL) at below 10° C. and extractedwith EtOAc (2×100 mL). The combined organic layer was dried over Na₂SO₄,filtered and concentrated in vacuum. The product was purified by flashcolumn (0˜10% of EtOAc in PE) to give 90.0 (1.7 g, 49%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.53 (t, J=8 Hz, 1H), 2.16-2.11 (m, 4H),2.04-1.98 (m, 1H), 1.83-1.52 (m, 3H), 1.50-1.30 (m, 5H), 1.27-1.02 (m,10H), 0.97-0.77 (m, 11H), 0.61 (s, 3H).

Synthesis of 90.1

To a solution of 90.0 (1.8 g, 5.2 mmol) and (1R)-1-phenylethan-1-amine(3.76 g, 31.1 mmol) in DCE (30 mL) at 20° C. was added NaBH₃CN (2.6 g,41.5 mmol) at 20° C. The suspension was stirred at 50° C. for 16 h. Thereaction was quenched with water (50 mL), extracted with DCM (2×50 mL).The combined organic phase was washed with brine (50 mL), dried overNa₂SO₄, filtered and concentrated under vacuum to give the product,which was purified by flash column (0˜30% of EtOAc in PE) to give 90.1(2.7 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.37-7.28 (m, 4H), 7.24-7.17 (m, 1H), 3.89(q, J=6.4 Hz, 1H), 2.76-2.64 (m, 1H), 2.22 (d, J=12.4 Hz, 1H), 1.87-1.50(m, 10H), 1.49-1.32 (m, 9H), 1.30-1.21 (m, 7H), 1.16-1.01 (m, 6H), 0.93(t, J=7.3 Hz, 3H), 0.88 (d, J=6.0 Hz, 3H), 0.78 (s, 3H).

Synthesis of 90.2

To a solution of 90.1 (2.7 g, 5.97 mmol) and Pd/C (300 mg, 10% Palladiumon carbon, 10% water dry) in MeOH (50 mL) at 20° C. was hydrogenatedunder 50 psi of hydrogen at 50° C. for 16 h. The reaction mixture wasfiltered through a pad of Celite and washed with MeOH (3×100 mL). Thefiltrate was concentrated to give 90.2 (1.4 g, 67.6%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.91-2.77 (m, 1H), 2.01-1.92 (m, 1H),1.80-1.51 (m, 10H), 1.49-1.27 (m, 12H), 1.23-1.04 (m, 8H), 1.00 (d,J=6.4 Hz, 3H), 0.93 (t, J=7.2 Hz, 3H), 0.73 (s, 3H).

Synthesis of 90

To a solution of 90.2 (100 mg, 0.3 mmol) in DMSO (3 mL) at 20° C. underN₂ were added 6-chloropyridine-3-carbonitrile (79.6 mg, 0.6 mmol) andDIPEA (74.3 mg, 0.6 mmol). After stirring at 120° C. for 16 h, themixture was extracted with EtOAc (3×50 mL). The combined organic phasewas washed with water (3×20 mL), brine (50 mL), dried over anhydrousNa₂SO₄, filtered and concentrated in vacuum. The residue was purified byflash column (10%-30% of EtOAc in PE) to give 90 (88.7 mg, 68.7%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.34 (d, J=2.0 Hz, 1H), 7.53 (d, J=8.8 Hz,1H), 6.29 (d, J=9.2 Hz, 1H), 4.89-4.69 (m, 1H), 3.95-3.72 (m, 1H),1.92-1.70 (m, 5H), 1.68-1.58 (m, 3H), 1.54-1.21 (m, 16H), 1.17-0.98 (m,8H), 0.93 (t, J=7.2 Hz, 3H), 0.63 (s, 3H). LC-ELSD/MS purity 99%,analytic SFC: 100% de. MS ESI calcd. for C₂₉H₄₄N₃O [M+H]⁺ 450.3, found450.3.

Example 91: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-13-methyl-17-((R)-1-((2-methyl-6-(3-methyl-1H-pyrazol-1-yl)pyridin-3-yl)amino)ethyl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(91)

To a solution of 90.2 (100 mg, 0.3 mmol),3-bromo-2-methyl-6-(3-methyl-1H-pyrazol-1-yl)pyridine (145 mg, 0.6mmol), BINAP (17.9 mg, 0.03 mmol) and t-BuOK (64.5 mg, 0.6 mmol) intoluene (3 mL) was added Pd(OAc)₂ (6.45 mg, 0.03 mmol) under N₂. Themixture was stirred at 110° C. under microwave for 18 h to give asolution. Water (10 mL) was added into the solution. The mixture wasextracted with EtOAc (3×20 mL). The combined organic phase was washedwith water (3×20 mL), brine (50 mL), dried over anhydrous Na₂SO₄,filtered, concentrated in vacuum. The residue was purified by flashcolumn (10%-30% of EtOAc in PE) to give 91 (40.6 mg, 27.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.26 (d, J=2.4 Hz, 1H), 7.56 (d, J=8.4 Hz,1H), 6.92 (d, J=8.4 Hz, 1H), 6.17 (d, J=2.4 Hz, 1H), 3.47-3.35 (m, 1H),3.33-3.24 (m, 1H), 2.36 (d, J=3.2 Hz, 6H), 2.14-2.06 (m, 1H), 1.95-1.58(m, 8H), 1.52-1.20 (m, 17H), 1.09 (d, J=6.0 Hz, 4H), 1.05-0.97 (m, 2H),0.93 (t, J=7.2 Hz, 3H), 0.66 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₃₃H₅₁N₄O [M+H]⁺ 519.4, found 519.4.

Example 92: Synthesis of 1-((R)-2-((3R, 5R, 8R, 9R, 10S, 13S, 14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile (92)

To a solution of C3 (90 mg, 0.2105 mmol) and Cs₂CO₃ (137 mg, 0.421 mmol)in DMF (3 mL) was added 1H-pyrazole-4-carbonitrile (39.1 mg, 0.421 mmol)at 25° C. under N₂. After stirring at 80° C. for 16 h, the solution wascooled to 25° C., poured into water (100 mL) and extracted with EtOAc(3×100 mL). The combined organic phase was washed with brine (2×50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by SFC (Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, 5 um)condition: 0.1% NH₃H₂O EtOH, flow rate: 40 mL/min) to give 92 (Peak 1,Rt=1.533 min, 30 mg) as a solid and 92a (Peak 2, Rt=1.936 min, 20 mg) asa solid. 92 was re-purified by HPLC separation (column: Xtimate C18150*25 mm*5 um, gradient: 85-100% condition: water (0.225% FA)-ACN, flowrate: 25 mL/min) to give 92 (7 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.77 (d, J=18 Hz, 2H), 4.50 (dd, J=4.4,13.2 Hz, 1H), 3.65 (dd, J=11.2, 13.2 Hz, 1H), 3.46-3.34 (m, 5H), 2.61(s, 1H), 2.17-2.03 (m, 1H), 2.17-2.03 (m, 1H), 1.93-1.73 (m, 5H), 1.66(br s, 1H), 1.55-1.29 (m, 8H), 1.26-0.99 (m, 9H), 0.79 (s, 3H), 0.67 (d,J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₀N₃O[M+H—H₂O]⁺ 422.3, found 422.3.

Example 93-64: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13R,14S,15S,17R)-15-cyclopropyl-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(93) &1-((S)-2-((3R,5R,8R,9R,10S,13R,14S,15S,17R)-15-cyclopropyl-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(94)&1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,15S,17S)-15-cyclopropyl-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-hydroxypropyl)-1H-pyrazole-4-carbonitrile(628)&1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,15S,17S)-15-cyclopropyl-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-hydroxypropyl)-1H-pyrazole-4-carbonitrile(629)

Synthesis of 93.1

To a solution of t-BuOK (6.17 g, 55.0 mmol) in THF (150 mL) was added93.0 (8 g, 27.5 mmol) at 25° C. under N₂. After stirring at 25° C. for10 min, methyl benzenesulfinate (8.59 g, 55.0 mmol) was added. Afterstirring at 30° C. for another 30 min, the mixture was quenched with H₂O(200 mL) and extracted with EtOAc (200×3 mL). The organic layer wasdried over Na₂SO₄, filtered and concentrated in vacuum to give 93.1 (16g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.74-7.44 (m, 8H), 3.53-3.44 (m, 1H), 3.26(dd, J=8.2, 9.9 Hz, 1H), 2.41-2.35 (m, 1H), 1.81 (br s, 1H), 1.56-1.30(m, 15H), 1.23-1.01 (m, 4H), 0.98 (s, 1H), 0.93 (s, 2H).

Synthesis of 93.2

To a mixture of 93.1 (16 g, 38.5 mmol) in xylene (200 mL) was addedNa₂CO₃ (61.1 g, 577 mmol) in portions. After stirring at 140° C. underN₂ for 12 h, the mixture was filtered and concentrated. The residue waspurified by flash column (0˜15% of EtOAc in PE) to give 93.2 (4.3 g) asa solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.55-7.51 (m, 1H), 6.03 (dd, J=3.1, 5.9Hz, 1H), 2.37 (br d, J=10.3 Hz, 1H), 1.85 (br s, 5H), 1.72 (br s, 2H),1.62-1.34 (m, 9H), 1.33-1.23 (m, 6H), 1.08 (s, 3H).

Synthesis of 93.3

To a solution of bromo (cyclopropyl) magnesium (6.14 g, 84.6 ml, 42.3mmol, 0.5 M in THF) in THF (150 mL) was added CuI (8.05 g, 42.3 mmol) at0° C. under N₂. After stirring at 0° C. for 1 h, 93.2 (3.5 g, 12.1 mmol)was added. After stirring at 0° C. for another 3 h, the residue waspoured into NH₄Cl (50 mL) and extracted with EtOAc (3×50 mL). Thecombined organic phase was washed with brine (2×50 mL), dried overanhydrous Na₂SO₄, filtered, concentrated. The residue was purified byflash column (0˜30% of EtOAc in PE) to give 93.3 (3.8 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.47-2.39 (m, 1H), 2.38-2.27 (m, 1H),1.96-1.69 (m, 8H), 1.63-1.48 (m, 6H), 1.45-1.43 (m, 1H), 1.40-1.31 (m,3H), 1.30-1.27 (m, 4H), 1.26-1.18 (m, 1H), 1.11 (s, 4H), 0.95 (br d,J=8.3 Hz, 1H), 0.70-0.62 (m, 1H), 0.47 (s, 1H), 0.24-0.03 (m, 2H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₂H₃₃N₃O[M−H₂O+H]⁺313.3 found313.3.

Synthesis of 93.4

To a mixture of EtPPh₃Br (20.6 g, 55.5 mmol) in THF (100 mL) was addedt-BuOK (6.22 g, 55.5 mmol) at 25° C. under N₂. After stirring at 45° C.for 30 min, 93.3 (3.7 g, 11.1 mmol) was added below 45° C. Afterstirring at 45° C. for another 16 h, the reaction mixture was quenchedwith 10% NH₄Cl aqueous (40 mL) at 25° C. and extracted with EtOAc (2×30mL). The combined organic phase was dried over Na₂SO₄, filtered,concentrated. The residue was purified by flash column (0˜20% of EtOAcin PE) to give 93.4 (3.7 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.18-5.07 (m, 1H), 2.46-2.36 (m, 1H),2.31-2.15 (m, 2H), 1.84 (br d, J=6.8 Hz, 4H), 1.77-1.63 (m, 4H),1.59-1.30 (m, 12H), 1.29-1.27 (m, 4H), 1.19-1.08 (m, 5H), 0.86-0.77 (m,1H), 0.58-0.49 (m, 1H), 0.40-0.31 (m, 1H), 0.13-0.00 (m, 2H).

Synthesis of 93.5

To a solution of 93.4 (700 mg, 2.04 mmol) in anhydrous THF (15 mL) wasadded BH₃.Me₂S (1.01 ml, 10.2 mmol) at 25° C. under N₂. After stirringat 25° C. for 12 h, the resulting mixture was treated sequentially withethanol (3.09 mL, 61.2 mmol) at 25° C., NaOH aqueous (12.2 mL, 5.0 M,61.2 mmol) and H₂O₂ (6.13 mL, 30% in water, 61.2 mmol) dropwise at 0° C.After stirring at 50° C. for 1 h, the mixture was cooled, poured intoNa₂S₂O₃ (50 mL, sat. aq.) and extracted with EtOAc (2×50 mL). Thecombined organic phase was washed with brine (30 mL), dried overanhydrous Na₂SO₄, filtered and concentrated in vacuum. The residue waspurified by flash column (15˜25% of EtOAc in PE) to give 93.5 (560 mg)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.82-3.74 (m, 1H), 2.24 (td, J=9.2, 13.5Hz, 1H), 2.02 (s, 1H), 1.85 (br d, J=6.5 Hz, 5H), 1.92-1.58 (m, 1H),1.92-1.58 (m, 1H), 1.41 (br d, J=3.3 Hz, 9H), 1.28 (s, 5H), 1.24 (d,J=6.3 Hz, 4H), 1.18-1.01 (m, 4H), 0.92-0.78 (m, 4H), 0.57 (br dd, J=3.9,7.7 Hz, 1H), 0.42-0.32 (m, 1H), 0.16-0.02 (m, 2H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₄H₃₇ [M-2H₂O+H]⁺325.3 found 325.3.

Synthesis of 93.6

To a mixture of 93.5 (460 mg, 1.27 mmol) in DCM (30 mL) was added DMP(1.61 g, 3.81 mmol) in portions. After stirring at 20° C. for 30 min,the mixture was quenched with NaHCO₃ (20 mL) and Na₂S₂O₃ (20 mL) andextracted with DCM (2×30 mL) The organic phase was washed with Na₂S₂O₃(2×20 mL, sat.), brine (30 mL, sat), dried over Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give 93.6 (310 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.44 (dd, J=8.8, 10.5 Hz, 1H), 2.14 (s,4H), 2.02-1.92 (m, 3H), 1.85 (br d, J=6.8 Hz, 2H), 1.76-1.65 (m, 2H),1.38 (br s, 12H), 1.29 (s, 4H), 1.17-1.04 (m, 2H), 0.87-0.77 (m, 1H),0.85 (s, 3H), 0.62-0.52 (m, 1H), 0.46-0.35 (m, 1H), 0.17-0.01 (m, 2H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₄H₃₇₀ [M−H₂O+H]⁺341.3 found341.3.

Synthesis of 93.7

To a suspension of Ph₃PMeBr (3.08 g, 8.64 mmol) in THF (20 mL) was addedt-BuOK (969 mg, 8.64 mmol) at 20° C. under N₂. After stirring for 30 minat 50° C., a solution of 93.6 (310 mg, 0.864 mmol) in THF (5 mL) wasadded dropwise to the resulting suspension. After stirring at 50° C. for2 h under N₂, the reaction mixture was poured into 10% NH₄Cl (50 mL) andextracted with EtOAc (40 mL×3). The combined organic phase was washedwith brine (40 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtOAcin PE) to give 93.7 (300 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.86 (s, 1H), 4.74 (s, 1H), 2.03-1.79 (m,7H), 1.78 (s, 3H), 1.76-1.60 (m, 3H), 1.51-1.27 (m, 13H), 1.23-0.98 (m,4H), 0.78 (s, 4H), 0.58 (br s, 1H), 0.45-0.32 (m, 1H), 0.17-0.03 (m,1H), 0.17-0.03 (m, 1H).

Synthesis of 93.8

To a solution of 93.7 (200 mg, 0.560 mmol) in THF (10 mL) was addedBH₃.Me₂S (0.559 mL, 10M, 5.59 mmol) at 50° C. After stirring for 16 h,to the resulting mixture was added ethanol (1.95 m, NaOH (6.70 mL, 5M inwater, 33.5 mmol) at 0° C. and hydrogen peroxide (3.32 mL, 10 M inwater, 33.5 mmol) dropwise at 0° C. After stirring at 80° C. for 1 h,the mixture was added into water (100 ml) and extracted with EtOAc(2×100 mL). The organic layer was washed with saturated Na₂S₂O₃ (100ml), brine (100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated in vacuum to give 93.8 (100 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.81-3.33 (m, 2H), 2.21-2.07 (m, 1H),2.02-1.52 (m, 10H), 1.51-1.44 (m, 3H), 1.39-1.29 (m, 5H), 1.28 (s, 4H),1.07 (br d, J=6.5 Hz, 7H), 0.97 (d, J=6.8 Hz, 2H), 0.90 (s, 4H),0.61-0.52 (m, 1H), 0.43-0.27 (m, 1H), 0.17-0.14 (m, 2H).

Synthesis of 93 & 94

To a solution of 93.8 (100 mg, 0.277 mmol) and1H-pyrazole-4-carbonitrile (51.5 mg, 0.554 mmol) in DMF (5 mL) wereadded Ph₃P (288 mg, 1.10 mmol) and DEAD (191 mg, 1.10 mmol). Afterstirring at 25° C. for 16 h, the mixture was poured into ice-water (30mL) and extracted with EtOAc (3×20 mL). The combined organic phase waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by SFC (Column: DAICEL CHIRALCELOD-H (250 mm*30 mm, 5 um); Condition: 0.1% NH₃H₂O ETOH; Begin B: 45%;End B: 45%) to afford 94 (30.6 mg, 25.7%, Rt=1.540 min) as a solid and93 (30.2 mg, 25.3%, Rt=1.957 min) as a solid.

93: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.76 (s, 1H), 4.48 (dd,J=4.8, 13.3 Hz, 1H), 3.70 (dd, J=10.3, 13.3 Hz, 1H), 2.23-2.08 (m, 2H),2.04-1.93 (m, 1H), 1.85 (br d, J=7.0 Hz, 4H), 1.77-1.59 (m, 1H),1.51-1.37 (m, 7H), 1.29 (s, 8H), 1.16-1.05 (m, 4H), 1.00 (s, 3H), 0.80(br s, 1H), 0.72 (d, J=6.5 Hz, 3H), 0.58 (br s, 1H), 0.42-0.31 (m, 1H),0.00-0.00 (m, 1H), 0.16-0.08 (m, 2H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₉H₄₂N₃[M−H₂O+H]⁺432.3 found 432.3. SFC 100% de.

94: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.78 (s, 1H), 4.30 (dd,J=3.8, 13.3 Hz, 1H), 3.73 (dd, J=9.9, 13.4 Hz, 1H), 2.26 (td, J=9.2,13.3 Hz, 1H), 2.14-2.03 (m, 1H), 1.84 (br d, J=6.8 Hz, 6H), 1.64-1.59(m, 2H), 1.39 (br d, J=10.8 Hz, 8H), 1.53-1.33 (m, 1H), 1.29-1.22 (m,5H), 1.08 (br d, J=10.5 Hz, 4H), 0.94 (s, 3H), 0.82 (d, J=6.5 Hz, 4H),0.59 (td, J=3.8, 7.9 Hz, 1H), 0.44-0.31 (m, 1H), 0.19-0.07 (m, 2H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂N₃[M−H₂O+H]⁺ 432.4 found432.4. SFC 96% de.

Example 95 & 96: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13R,14S,15R,17R)-3-hydroxy-3,13,15-trimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(95) &1-((S)-2-((3R,5R,8R,9R,10S,13R,14S,15R,17R)-3-hydroxy-3,13,15-trimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(96)

Synthesis of 95.1

To a solution of MeMgBr (2.3 mL, 6.92 mmol, 3M) in THF (10 mL) was addedCuI (988 mg, 5.19 mmol) at 0° C. After stirring at 0° C. for 1 h, 93.2(500 mg, 1.73 mmol) in THF (5 mL) was added at 0° C. After stirring at0° C. for 3 h, the mixture was poured into saturated NH₄Cl (20 mL) andextracted with EtOAc (3×30 mL). The combined organic layer was washedwith brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (10%-25% of EtOAcin PE) to give 95.1 (360 mg, 68.4%, 35.2 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.51-2.39 (m, 2H), 2.29-2.19 (m, 1H),1.91-1.80 (m, 3H), 1.78-1.62 (m, 4.5H), 1.53-1.46 (m, 2.5H), 1.44-1.31(m, 7H), 1.28 (s, 5H), 1.24-1.20 (m, 1H), 1.10 (d, J=7.6 Hz, 3H), 1.03(s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₀H₃₁O[M−H₂O+H]⁺287.2 found 287.2.

Synthesis of 95.2

To a mixture of EtPPh₃Br (18.2 g, 49.2 mmol) in THF (40 mL) was addedt-BuOK (5.52 g, 49.2 mmol) at 20° C. under N₂. After stirring at 40° C.for 30 min, 95.1 (2.5 g, 8.21 mmol) in THF (30 mL) was added in portionsbelow 40° C. After stirring at 40° C. for 16 h, the reaction mixture wasquenched with 10% NH₄Cl aqueous (200 mL) at 15° C. and extracted withEtOAc (3×200 mL). The combined organic phase was washed with brine(2×150 mL), filtered, concentrated under vacuum. The residue waspurified by flash column (0˜30% ethyl acetate in PE) to give 95.1 (3.1g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.18-5.07 (m, 1H), 2.63-2.50 (m, 1H),2.33-2.23 (m, 3H), 2.22-2.06 (m, 3H), 1.91-1.79 (m, 3H), 1.66 (s, 7H),1.61-1.31 (m, 11H), 1.25-1.13 (m, 7H), 1.09 (s, 3H), 0.93 (m, 3H).

Synthesis of 95.3

To a solution of 95.2 (2.6 g, 8.21 mmol) in anhydrous THF (30 mL) wasadded 9-BBN dimer (4.00 g, 16.4 mmol) at 25° C. under N₂. After stirringat 40° C. for 16 h, to the resulting mixture was added ethanol (4.53 g,98.5 mmol) at 25° C., followed by NaOH aqueous (19.7 mL, 5.0 M, 98.5mmol) and H₂O₂ (9.85 mL, 10 M, 98.5 mmol) dropwise at 0° C. Afterstirring at 80° C. for 1 h, the mixture was cooled, poured into Na₂S₂O₃(100 mL, sat. aq.) and extracted with EtOAc (2×150 mL). The organicphase was washed with brine (2×100 mL), dried over anhydrous Na₂SO₄,filtered and concentrated in vacuum. The residue was purified by flashcolumn (15-40% EtOAc in PE) to give 95.3 (2.6 g, 94.8%) as a solid

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.86-3.65 (m, 1H), 2.38-2.26 (m, 1H),2.20-2.07 (m, 1H), 1.91-1.52 (m, 11H), 1.50-1.37 (m, 6H), 1.29-1.24 (m,8H), 1.19-0.98 (m, 5H), 0.93 (m, 3H), 0.82 (s, 3H).

Synthesis of 95.4

To a solution of 95.3 (2.6 g, 7.77 mmol) in DCM (30 mL) was addedDess-martin (6.57 g, 15.5 mmol) at 25° C. After stirring at 25° C. for10 min, the mixture was quenched with saturated NaHCO₃ aqueous (100 mL)at 10° C. The DCM phase was separated and washed with saturatedNaHCO₃/Na₂S₂O₃ aqueous (1:1, 3×100 mL), brine (2×50 mL), dried overNa₂SO₄, filtered and concentrated under vacuum. The residue was purifiedby flash column (0˜30% of EtOAc in PE) to give 95.4 (1 g, 38.7%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.49 (dd, J=8.8, 10.8 Hz, 1H), 2.22-2.13(m, 1H), 2.11 (s, 3H), 2.09-2.00 (m, 1H), 1.97-1.79 (m, 5H), 1.75-1.59(m, 3H), 1.51-1.29 (m, 9H), 1.28 (s, 4H), 1.25-0.99 (m, 3H), 0.96 (d,J=7.2 Hz, 3H), 0.78 (s, 3H). LC-ELSD/MS purity: 99%, MS ESI calcd. forC₂₂H₃₆O₂[M−H₂O+H]⁺315.3, found C₂₂H₃₆O₂[M−H₂O+H]⁺315.2.

Synthesis of 95.5

To a mixture of MePPh₃Br (2.24 g, 6.30 mmol) in THF (27 mL) was addedt-BuOK (706 mg, 6.30 mmol) at 20° C. under N₂. After stirring at 50° C.for 30 min, 95.4 (700 mg, 2.10 mmol) in THF (3 mL) was added in portionsbelow 50° C. After stirring at 50° C. for 16 h, the reaction mixture wasquenched with 10% NH₄Cl aqueous (20 mL) at 15° C. and extracted withEtOAc (3×20 mL). The combined organic phase was washed with brine (2×20mL), dried over anhydrous Na₂SO₄, filtered and concentrated undervacuum. The residue was purified by flash column (0˜20% of ethyl acetatein PE) to give 95.5 (620 mg, 89.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.71 (s, 1H), 2.16-1.95 (m,3H), 1.91-1.77 (m, 4H), 1.76 (s, 3H), 1.69-1.58 (m, 3H), 1.49-1.39 (m,5H), 1.37-1.28 (m, 4H), 1.27 (s, 4H), 1.24-0.99 (m, 6H), 0.95 (d, J=7.2Hz, 3H), 0.91-0.82 (m, 2H), 0.72 (s, 3H).

Synthesis of 95.6

To a solution of 95.5 (300 mg, 0.9075 mmol) in THF (5 mL) was addedBH₃-Me₂S (544 μL, 10M, 5.44 mmol) at 25° C. After stirring at 45° C. for16 h, the resulting mixture was treated with ethanol (1.25 g, 27.2 mmol)at 15° C. and by NaOH aqueous (5.43 mL, 5.0 M, 27.2 mmol) at 0° C.Hydrogen peroxide (2.71 mL, 10 M, 27.2 mmol) was then added dropwise at0° C. After stirring at 78° C. for 1 h, the mixture was cooled to 15° C.and Na₂S₂O₃ (20 mL, sat. aq.) was added. The aqueous layer was extractedwith EtOAc (3×20 mL). The organic phase was washed with brine (50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜40% of EtOAc in PE) to give 95.6 (450 mg) asa solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.59 (m, 1H), 3.51-3.34 (m, 1H),2.33-2.16 (m, 1H), 2.15-1.99 (m, 1H), 1.95-1.79 (m, 3H), 1.72-1.55 (m,6H), 1.51-1.36 (m, 5H), 1.27 (s, 4H), 1.24-1.08 (m, 6H), 1.05 (br d,J=6.8 Hz, 3H), 0.96 (d, J=6.8 Hz, 2H), 0.90 (dd, J=2.0, 7.2 Hz, 3H),0.84 (s, 3H).

Synthesis of 95.7

To a solution of 95.6 (250 mg, 0.7172 mmol) in DCM (5 mL) were addedN-Me-imidiazole (87.8 mg, 1.07 mmol), TEA (144 mg, 1.43 mmol) and TsCl(203 mg, 1.07 mmol). After stirring at 20° C. for 1 h, the mixture waswashed with water (5 mL) and extracted with DCM (3×20 mL). The organicphase was washed with brine (50 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜40% of EtOAc in PE) to give 95.7 (240 mg, 66.6%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (dd, J=2.4, 8.4 Hz, 2H), 7.34 (dd,J=2.4, 8.4 Hz, 2H), 4.18-4.07 (m, 1H), 4.01-3.94 (m, 1H), 3.82-3.74 (m,1H), 2.45 (s, 3H), 2.23-2.05 (m, 1H), 1.88-1.76 (m, 4H), 1.73-1.55 (m,3H), 1.53-1.32 (m, 7H), 1.29-1.23 (m, 6H), 1.23-1.01 (m, 6H), 1.00-0.93(m, 3H), 0.90-0.84 (m, 5H), 0.80-0.71 (m, 3H).

Synthesis of 95 & 96

To a solution of 95.7 (240 mg, 0.4773 mmol) in DMF (5 mL) were added4-cyano-pyrazole (53.3 mg, 0.5727 mmol), KI (79.2 mg, 0.4773 mmol) andCs₂CO₃ (465 mg, 1.43 mmol). After stirring at 120° C. for 16 h, themixture was quenched with water (5 mL) and extracted with EtOAc (50mL×3). The organic phase was washed with brine (30 mL×2), dried overNa₂SO₄, filtered and concentrated under vacuum. The residue was purifiedby column (0%-55% of EtOAc in PE) to give a mixture of diastereomers.The diastereomers were separated by SFC (column: DAICEL CHIRALCEL OD-H(250 mm*30 mm, 5 um); Mobile phase: A: CO₂ B: 0.1% NH₃H₂O ETOH;gradient: from 40% to 40% of B, Flow Rate (ml/min): 60) to give 96 (51.5mg, 21.5%) and 95 (65.7 mg, 27.4%) as a solid.

95: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.76 (s, 1H), 4.27 (dd,J=3.76, 13.30 Hz, 1H), 3.72 (dd, J=9.91, 13.43 Hz, 1H), 2.43-2.27 (m,1H), 2.22-1.98 (m, 2H), 1.93-1.79 (m, 4H), 1.73-1.53 (m, 6H), 1.51-1.36(m, 5H), 1.27 (s, 3H), 1.22-1.00 (m, 7H), 0.94 (d, J=7.2 Hz, 3H), 0.87(s, 3H), 0.80 (d, J=6.5 Hz, 2H). LC-ELSD/MS purity: 99%, MS ESI calcd.for C₂₇H₄₁N₃O [M−H₂O+H]⁺406.3, found C₂₇H₄₁N₃O [M−H₂O+H]⁺406.3.

96: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.47 (dd,J=4.5, 13.3 Hz, 1H), 3.68 (dd, J=10.4, 13.2 Hz, 1H), 2.28-2.06 (m, 3H),1.88-1.57 (m, 8H), 1.51-1.34 (m, 6H), 1.27 (s, 4H), 1.21-1.02 (m, 7H),0.97-0.89 (m, 6H), 0.69 (d, J=6.8 Hz, 3H). LC-ELSD/MS purity: 99%, MSESI calcd. for C₂₇H₄₁N₃O [M−H₂O+H]⁺406.3, found C₂₇H₄₁N₃O[M−H₂O+H]⁺406.3.

The following examples were synthesized similar to Examples 29, 30, 31,or 32 with the listed acid and appropriate SM. In the case of B24 as SM,the diastereomeric products were separated by SFC (e.g. Column: DAICELCHIRALPAK AS-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃H₂O EtOH, BeginB: 30%, End B 30%) yielding both diastereomers after separation. Thediastereomers were assigned based on 1H NMR of C21-Me.

Example SM acid STRUCTURE Analytical 100 B18 pyridine- 2- carboxylicacid

¹H NMR (400 MHz, CDCl3) δ_(H) 8.55 (d, J = 4.0 Hz, 1H), 8.21 (d, J = 7.6Hz, 1H), 7.95 (d, J = 9.6 Hz, 1H), 7.84 (dt, J = 2.0, 8.0 Hz, 1H),7.43-7.38 (m, 1H), 4.19-4.07 (m, 1H), 1.86-1.74 (m, 5H), 1.67-1.46 (m,4H), 1.45-1.31 (m, 7H), 1.31- 1.19 (m, 6H), 1.19 (d, J = 6.4 Hz, 3H),1.16-0.86 (m, 6H), 0.71 (s, 3H); LC-ELSD/MS purity 95%, MS ESI calcd.for C₂₇H₄₁N₂O₂ [M + H]⁺ 425, found 425. 101 B20 pyridine- 2- carboxylicacid

¹H NMR (400 MHz, CDCl3) δ_(H) 8.53 (d, J = 4.0 Hz, 1H), 8.19 (d, J = 8.0Hz, 1H), 7.93 (d, J = 9.2 Hz, 1H), 7.83 (dt, J = 1.6, 7.6 Hz, 1H),7.43-7.38 (m, 1H), 4.24-4.14 (m, 1H), 1.99-1.93 (m, 1H), 1.89-1.79 (m,4H), 1.68-1.57 (m, 7H), 1.50- 1.37 (m, 7H), 1.29-1.26 (m, 7H), 1.14-1.02(m, 5H), 0.77 (s, 3H); LC- ELSD/MS purity 99%, MS ESI calcd. forC₂₇H₄₁N₂O₂ [M + H]⁺ 425, found 425. 102 B20 2- fluorobenzoic acid

¹H NMR (400 MHz, CDCl3) δ 8.15- 7.95 (m 1H), 7.51-7.36 (m, 1H),7.26-7.20 (m, 1H), 7.16-7.03 (m, 1H), 7.02-7.00 (m, 1H), 4.25-4.20 (m,1H), 2.00-1.75 (m, 4H), 1.71- 1.54 (m, 5H), 1.53-1.35 (m, 7H), 1.33-1.18(m, 10H), 1.17-1.00 (m, 5H), 0.75 (s, 3H); LCMS purity 99%, MS ESIcalcd. For C28H40FNO2 [M + H]⁺ 442, found 442. 103 B18 2- fluorobenzoicacid

¹H NMR (400 MHz, CDCl3) δ 8.28-8.24 (m 1H), 7.65-7.52 (m, 1H), 7.39-7.40(m 1H), 7.26-7.24 (m, 1H), 6.85-6.65 (m, 1H), 4.46- 4.21 (m, 1H),2.05-1.93 (m, 5H), 1.81-1.66 (m, 4H), 1.60-1.52 (m, 7H), 1.43-1.29 (m,10H), 1.27-1.08 (m, 5H), 0.86 (s, 3H); LCMS purity 98%, MS ESI calcd.For C28H40FNO2 [M + H]⁺ 442, found 442 104 B18 3- methoxybenzoic acid

¹H NMR (400 MHz, CDCl3) δ 7.37-7.31 (m, 2 H), 7.23-7.21 (m, 1 H),7.04-6.99 (m, 1 H), 5.89-5.86 (m, 1 H), 4.22-4.18 (m, 1 H), 3.85 (s,3H), 1.89-1.64 (m, 5 H), 1.63- 1.48 (m, 3 H), 1.50-1.39 (m, 8 H),1.38-1.15 (m, 10 H), 1.14-0.95 (m, 5 H), 0.74 (s, 3 H); LCMS purity 99%,MS ESI calcd. For C29H43NO3 [M + H]⁺ 454, found 454.3 105 B20 3-methoxybenzoic acid

¹H NMR (400 MHz, CDCl3) δ 7.34-7.30 (m, 2 H), 7.23-7.20 (m, 1 H),7.04-6.98 (m, 1 H), 5.95-5.80 (m, 1 H), 4.27-4.12 (m, 1 H), 3.85 (s,3H), 1.98-1.77 (m, 4 H), 1.75- 1.60 (m, 4 H), 1.55-1.33 (m, 8 H),1.32-1.15 (m, 10 H), 1.14-0.98 (m, 5 H), 0.77 (s, 3 H); LCMS purity 99%,MS ESI calcd. For C29H43NO3 [M + H]⁺ 454, found 454.3 106 B18 4-methoxybenzoic acid

¹H NMR (400 MHz, CDCl3) δ 7.76-7.65 (m, 2 H), 6.98-6.88 (m, 2 H),5.84-5.76 (m, 1 H), 4.24-4.11 (m, 1 H), 3.84 (s, 3 H), 1.92-1.72 (m, 5H), 1.70-1.58 (m, 3 H), 1.49- 1.32 (m, 8 H), 1.31-1.23 (m, 6 H),1.22-1.09 (m, 6 H), 1.08-0.90 (m, 3 H), 0.73 (s, 3 H); LCMS purity 99%,MS ESI calcd. For C₂₉H₄₃NO₃ [M + H]⁺ 454, found 454 107 B20 4-methoxybenzoic acid

¹H NMR (400 MHz, CDCl3) δ 7.73-7.68 (m, 2 H), 6.96-6.88 (m, 2 H),5.84-5.76 (m, 1 H), 4.27 −4.15 (m, 1 H), 3.84 (s, 3 H), 1.78-1.98 (m, 5H), 1.73-1.58 (m, 3 H) 1.53- 1.30 (m, 9 H), 1.29-1.22 (m, 8 H),1.21-1.01 (m, 6 H), 0.76 (s, 3 H); LCMS purity 99%, MS ESI calcd.C29H43NO3 [M + H]⁺ 454, found 454 108 B18 4-cyanobenzoic acid

¹H NMR (400 MHz, CDCl₃)δ 7.89- 7.82 (m, 2H), 7.77-7.71(m, 2H), 5.94-5.87(m, 1H), 4.25-4.15 (m, 1H), 1.89- 1.74(m, 5H), 1.72-1.55(m, 5H),1.48- 1.34(m, 8H), 1.29-1.15(m, 8H), 1.15- 0.95(m, 5H), 0.74(s, 3H);LCMS purity 99%, MS ESI calcd. For C₂₉H₄₁N₂O₂ [M + H]⁺ 449, found 449.109 B19 3-cyanobenzoic acid

¹H NMR (400 MHz, CDCl3) δ_(H) 8.06-7.95 (m, 2H), 7.78 (d, 1H), 7.61-7.55(m, 1H), 5.88 (d, 1H), 4.25-4.14 (m, 1H), 1.90-1.74 (m, 5H), 1.71-1.59(m, 3H), 1.50-1.34 (m, 9H), 1.33-1.27 (m, 2H), 1.26 (s, 3H), 1.24-1.21(m, 1H), 1.19 (d, 3H), 1.16-0.97 (m, 5H), 0.74 (s, 3H); LCMS MS ESIcalcd. for C₂₉H₄₁N₂O₂ [M + H]+ 449, found 449. 110 B20 3-cyanobenzoicacid

H NMR (400 MHz, CDCl3) δ 8.03- 7.93 (m, 2H), 7.77 (d, 1H), 7.60- 7.53(m, 1H), 5.91 (d, 1H), 4.29- 4.14 (m, 1H), 1.95 (d, 1H), 1.90- 1.76 (m,4H), 1.71-1.52 (m, 6H), 1.51-1.31 (m, 8H), 1.28 (s, 3H), 1.27-1.22 (m,3H), 1.19-0.99 (m, 6H), 0.77 (s, 3H); LCMS MS ESI calcd. for C₂₉H₄₁N₂O₂[M + H]+ 449, found 449. 111 B24 4- (trifluoro methoxy) benzoic acid

B38: ¹H NMR (400 MHz, CDCl3) δ 7.77 (d, J = 8.8 Hz, 2H), 7.27-7.24 (m,2H), 5.84 (d, J = 9.2 Hz, 1H), 4.18 (br s, 1H), 3.45-3.35 (m, 5H),2.00-1.52 (m, 9H), 1.52-1.25 (m, 8H), 1.25-1.00 (m, 11H), 0.76 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₃F₃NO₄ [M + H]⁺ 538, found538. SFC 100% de. 112

¹H NMR (400 MHz, CDCl3) δ 7.77 (d, J = 8.8 Hz, 2H), 7.27-7.24 (m, 2H),5.87 (d, J = 8.8 Hz, 1H), 4.17 (br s, 1H), 3.45-3.35 (m, 5H), 2.62 (s,1H), 1.85-1.56 (m, 9H), 1.50- 1.25 (m, 8H), 1.25-0.95 (m, 10H), 0.73 (s,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₃F₃NO₄ [M + H]⁺ 538,found 538. SFC 100% de. 113 B24 4- fluorobenzoic acid

¹H NMR (400 MHz, CDCl3) δ 7.77-7.73 (m, 2H), 7.10 (T, J = 8.4 Hz, 2 H),5.86 (d, J = 8.8 Hz, 1H), 4.20-4.14 (m, 1H), 3.41-3.34 (m, 5H), 2.63 (s,1H), 1.84-1.53 (m, 10H), 1.47-1.29 (m, 8H), 1.23-0.93 (m, 9H), 0.72 (s,3H). LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₃FNO₃ [M + H]⁺ 472,found 472. SFC 96.96% de. 114

¹H NMR (400 MHz, CDCl3) δ 7.75-7.71 (m, 2H), 7.10 (t, J = 8.4 Hz, 2H),5.86 (d, J = 8.8 Hz, 1H), 4.23-4.14 (m, 1H), 3.42-3.36 (m, 5H), 2.63 (s,1H), 1.95-1.56 (m, 10H), 1.50-1.32 (m, 8H), 1.28-1.00 (m, 9H), 0.75 (s,3H). LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₃FNO₃ [M + H]⁺ 472,found 472. SFC 95.44% de. 115 B20 4- cyanobenzoic acid

¹H NMR (400 MHz, CDCl3) δ_(H) 7.85-7.79 (m, 2H), 7.76-7.70 (m, 2H), 5.92(br d, J = 8.8 Hz, 1H), 4.26- 4.15 (m, 1H), 1.98-1.90 (m, 1H), 1.88-1.77(m, 4H), 1.69-1.54 (m, 7H), 1.49-1.38 (m, 7H), 1.27 (t, J = 3.6 Hz, 7H),1.20-1.06 (m, 5H), 0.77 (s, 3H); LCMS purity 99%, MS ESI calcd. forC29H41N2O2 [M + H]+ 449, found 449. 116 B24 4- fluorobenzoic acid

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.75 (dd, J = 5.3, 8.8 Hz, 2H), 7.10 (t, J= 8.5 Hz, 2H), 5.84 (br d, J = 9.0 Hz, 1H), 4.30-4.08 (m, 1H), 1.83-1.79(m, 5H), 1.70-1.54 (m, 4H), 1.49- 1.22 (m, 14H), 1.22-1.01 (m, 8H), 0.73(s, 3H). ¹⁹F NMR (376 MHz, CDCl₃): δ_(F) −108.682. LC- ELSG/MS purity99%, MS ESI calcd. For C₂₈H₄₁FNO₂ [M + H]⁺ 442, found 442. SFC 98.25%de. 117

¹H NMR (400 MHz, CDCl3) δ_(H) 7.81-7.64 (m, 2H), 7.09 (t, J = 8.7 Hz,2H), 5.83 (br d, J = 8.5 Hz, 1H), 4.31- 4.10 (m, 1H), 1.94 (br d, J =12.3 Hz, 1H), 1.89-1.75 (m, 4H), 1.51-1.40 (m, 6H), 1.50-1.40 (m, 5H),1.33- 1.22 (m, 9H), 1.20-0.99 (m, 6H), 0.76 (s, 3H). ¹⁹F NMR (376 MHz,CDCl₃): δ_(F) −108.774. LC- ELSG/MS purity 99%, MS ESI calcd. ForC₂₈H₄₁FNO₂ [M + H]⁺ 442, found 442. SFC 95.16% de 118 B24 1,3- dimethyl-1H- pyrazole- 5- carboxylic acid

¹H NMR (400 MHz, CDCl3) δ_(H) 6.17 (s, 1H), 5.68 (br d, J = 9.3 Hz, 1H),4.11-4.02 (m, 4H), 3.46-3.30 (m, 5H), 2.62 (br s, 1H), 2.26 (s, 3H),1.88-1.69 (m, 6H), 1.62-1.56 (m, 5H), 1.46-0.94 (m, 16H), 0.71 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. For C₂₈H₄₆N₃O₃ [M + H]⁺ 472, found472. SFC 99.4% de. 119

¹H NMR (400 MHz, CDCl3) δ_(H) 6.20 (s, 1H), 5.71 (br d, J = 9.0 Hz, 1H),4.19-4.02 (m, 4H), 3.47-3.32 (m, 5H), 2.25 (s, 3H), 1.96-1.88 (m, 1H),1.86-1.65 (m, 7H), 1.51-0.98 (m, 20H), 0.74 (s, 3H). LC- ELSD/MS purity99%, MS ESI calcd. For C₂₈H₄₆N₃O₃ [M + H]⁺ 472, found 472. SFC 98.62%de. 120 B24 5- cyanopyridine- 2- carboxylic acid

B47: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.83 (d, J = 1.0 Hz, 1 H), 8.34 (d, J= 8.0 Hz, 1 H), 8.13 (dd, J = 2.0, 8.4 Hz, 1 H), 7.85 (d, J = 9.2 Hz, 1H), 4.20-4.04 (m, 1 H), 1.84 (s, 1 H), 1.82-1.71 (m, 1 H), 1.82-1.68 (m,4 H), 1.48-1.31 (m, 10 H), 1.28- 1.22 (m, 6 H), 1.19 (d, J = 6.4 Hz, 4H), 1.22-1.17 (m. 1 H), 1.14-1.09 (m, 2 H), 0.99-0.82 (m, 2 H), 0.69 (s,3 H). LC-ELSD/MS purity 99% MS ESI calcd. For C₂₇H₄₀N₄O₃ [M + H]⁺ 450,found 450. SFC 99.5% de 121

¹H NMR (400 MHz, CHLOROFORM-d) Shift = 8.81 (d, J = 1.2 Hz, 1H), 8.32(d, J = 8.0 Hz, 1H), 8.13 (dd, J = 2.0, 8.0 Hz, 1H), 7.83 (d. J = 9.2Hz. 1H). 4.28-4.10 (m, 1H), 1.95 (d, J = 12.4 Hz, 1H), 1.89-1.77 (m.4H), 1.70-1.62 (m. 2H), 1.55-1.35 (m, 10H), 1.32-1.22 (m. 10H).1.19-0.99 (m, 6H), 0.76 (s, 3H). LC-ELSD/MS purity 99% MS ESI calcd. ForC₂₇H₄₀N₄O₃ [M + H]⁺ 450. found 450. SFC 99.3% de 122 B24 6-cyanopyridine- 3- carboxylic

¹H NMR (400 MHz, CDCl₃) δ_(H) 9.02 (d, J = 1.6 Hz, 1H), 8.24 (dd, J =2.4, 8.0 Hz, 1H), 7.79 (d, J = 8.0 Hz, 1H), 5.99 (br d, J = 9.2 Hz, 1H),4.36 − 4.06 (m, 1H), 3.46 − 3.31 (m. 5H), 2.64 (s, 1H), 1.90-1.81 (m.1H), 1.81 − 1.72 (m. 4H), 1.65 (br d. J = 4.8 Hz, 2H). 1.51 − 1.29 (m.9H). 1.29- 1.19 (m, 5H), 1.13 (brd, J = 9.6 Hz. 3H). 1.09 − 0.93 (m,3H). 0.73 (s, 3H); LC-ELSD/MS purity 99%, MS ESI calcd. for C29H40N3O2[M + H]+ 462, found 462 123

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.92 (d, J = 1.6 Hz, 1H), 8.14 (dd, J =2.0, 8.0 Hz, 1H), 7.71 (d, J = 8.0 Hz, 1H), 5.90 (br d, J = 8.8 Hz, 1H),4.30 − 4.08 (m, 1H), 3.40 − 3.27 (m, 5H), 2.56 (s, 1H), 1.87 (br d, J =12.3 Hz, 1H), 1.81 − 1.67 (m, 4H), 1.61 (br s, 2H), 1.44 − 1.25 (m, 9H),1.25 − 1.15 (m, 5H), 1.14 − 1.05 (m, 3H), 1.04 − 0.93 (m, 3H), 0.70 (s,3H); LC-ELSD/MS purity 99%, MS ESI calcd. for C29H41N3O2 [M + H]+ 462,found 462. 124 B20 5- fluoropyridine- carboxylic acid

1HNMR (400 MHz, CHLOROFORM-d) δ 8.36 (d, J = 2.8 Hz, 1H), 8.23 (dd, J =4.6, 8.7 Hz, 1H), 7.74 (br d, J = 9.0 Hz, 1H), 7.52 (dt, J = 2.8, 8.3Hz, 1H), 4.26-4.00 (m, 1H), 1.96 (br d, J = 12.5 Hz, 1H), 1.90-1.66 (m,5H), 1.52-1.20 (m, 20H), 1.20-0.94 (m, 5H), 0.76 (s, 3H). 19F NMR (376MHz, CHLOROFORM-d) δ −123.06 (s, 1F). LC-ELSD/MS purity 99%, MS ESIcalcd. for C27H40FN2O2 [M + H]+ 443, found 443. 125 B24 5-fluoropyridine- carboxylic acid

¹H NMR (400 MHz, CDCl3) δ 8.39-8.36 (m, 1H), 8.23-8.21 (m, 1H),7.80-7.71 (m, 1H), 7.52 (m, 1H), 4.22-4.03 (m, 1H),3.47-3.30 (m, 6H),2.60 (m, 1H), 1.62-1.92 (m, 7H), 1.54-1.32 (m, 8H), 1.30-1.16 (m, 6H),1.14-0.87 (m, 5H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.For C₂₈H₄₂FN₂O₃ [M + H]⁺ 473, found 473. SFC 99% de. 126

¹H NMR (400 MHz, CDCl3) δ 8.39-8.36 (m, 1H), 8.23-8.21 (m, 1H),7.80-7.71 (m, 1H), 7.52 (m, 1H), 4.22-4.03 (m, 1H), 3.47-3.30 (m, 6H),2.60 (m, 1H), 1.62-1.92 (m, 7H), 1.54-1.32 (m, 10H), 1.30-1.16 (m, 6H),1.14-0.87 (m, 5H), 0.76 (s, 3H). LC-ELSD/MS purity 99% MS ESI calcd. ForC₂₈H₄₂FN₂O₃ [M + H]⁺ 473, found 473. SFC 99% de. 127 B18 5-fluoropyridine- carboxylic acid

¹H NMR (400 MHz, CDCl₃) δ 8.38 (m, 1H), 8.28-8.19 (m, 1H), 7.79- 7.72 m,1H), 7.57-7.47 (m, 1H), 4.18-4.04 (m, 1H), 1.90-1.73 (m, 5H), 1.69-1.59(m, 7H), 1.49-1.39 (m, 3H), 1.34-1.22 (m, 7H), 1.21- 1.15 (m, 4H),1.14-0.87 (m, 5H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.For C₂₇H₄₀FN₂O₂ [M + H]⁺ 443, found 443. 128 B24 5- (trifluoromethoxy)pyridine-2- carboxylic acid

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.47 (d, J = 2.8 Hz, 1H), 8.28 (d, J = 8.8Hz, 1H), 7.77 (d, J = 9.2 Hz, 1H), 7.69-7.66 (m, 1H), 4.15-4.06 (m, 1H),3.43-3.34 (m, 5H), 2.59 (s, 1H), 1.89-1.69 (m, 5H), 1.66-1.51 (m, 7H),1.48-1.29 (m, 8H), 1.27- 1.09 (m, 5H), 1.06-0.85 (m, 2H), 0.69 (s, 3H).¹⁹F NMR (367 MHz, CDCl₃) δ −57.98. LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₉H₄₂F₃N₂O₄ [M + H]⁺ 539, found 539. SFC 100% de. 129

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.45 (d, J = 2.8 Hz, 1H), 8.27 (d, J = 8.8Hz, 1H), 7.78 (d, J = 9.2 Hz, 1H), 7.69-7.67 (m, 1H), 4.15-4.06 (m, 1H),3.43-3.34 (m, 5H), 2.60 (s, 1H), 1.97-1.71 (m, 5H), 1.68-1.53 (m, 7H),1.52-1.32 (m, 8H), 1.27- 0.99 (m, 7H), 0.76 (s, 3H). ¹⁹F NMR (367 MHz,CDCl₃) δ −58.03. LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂F₃N₂O₄[M + H]⁺ 539, found 539. SFC 95.4% de. 130 B18 4-cyano- 2- fluorobenzoicacid

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.25 (t, J = 7.6 Hz, 1H), 7.59-7.55 (m,1H), 7.46-7.42 (m, 1H), 6.60-6.52 (m, 1H), 4.25-4.16 (m, 1H), 1.90-1.75(m, 5H), 1.70-1.56 (m, 3H), 1.56- 1.30 (m, 8H), 1.30-0.95 (m, 15H), 0.72(s, 3H). ¹⁹F NMR (376 MHz, CDCl₃) δ_(F) −111.418. LC-ELSD/MS purity 99%,MS ESI calcd. for C₂₉H₄₀FN₂O₂ [M + H]⁺ 467, found 467. SFC 100% de. 131E2 4-cyano- 2- fluorobenzoic acid

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.25 (t, J = 8.0 Hz, 1H), 7.57 (dd, J =1.2, 8.0 Hz, 1H), 7.44 (dd, J = 1.2, 11.2 Hz, 1H), 6.60 − 6.51 (m, 1H),4.25 − 4.15 (m, 1H), 3.42 − 3.35 (m, 5H), 2.61 (s, 1H), 1.87 − 1.60 (m,7H), 1.50 1.23 (m, 10H), 1.19 (d, J = 6.4 Hz, 4H), 1.12 (m, 6H), 0.72(s, 3H). ¹⁹F NMR (376 MHz, CDCl₃) δ_(F) −111.34. LC-ELSD/MS purity 99%,MS ESI calcd. for C₃₀H₄₂FN₂O₃ [M + H]⁺ 497, found 497. 132 F8 2,4-difluorobenzoic acid

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.20- 8.13 (m, 1H), 7.02-6.96 (m, 1H),6.89-6.82 (m, 1H), 6.56-6.47 (m, 1H), 4.24-4.14 (m, 1H), 3.55-3.49 (m,2H), 3.45-3.37 (m, 2H), 2.71 (s, 1H), 1.86-1.59 (m, 8H), 1.50-1.28 (m,9H), 1.22-1.17 (m, 7H), 1.16- 0.90 (m, 6H), 0.72 (s, 3H). ¹⁹F NMR (376MHz, CDCl₃) δ_(F) −104.56, −104.59, −109.37, −109.40. LC- ELSD/MS purity99%, MS ESI calcd. for C₃₀H₄₄F₂NO₃ [M + H]⁺ 504, found 504; SFC 99% de.133 F8 4-cyano- 2- fluorobenzoic acid

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.25 (t, J = 8.0 Hz, 1H), 7.57 (dd, J =1.2, 8.0 Hz, 1H), 7.44 (d, J = 11.2 Hz, 1H), 6.60-6.51 (m, 1H),4.26-4.14 (m, 1H), 3.55-3.49 (m, 2H), 3.45- 3.37 (m, 2H), 2.72 (s, 1H),1.85-1.73 (m, 4H), 1.67-1.61 (m, 3H), 1.49- 1.33 (m, 10H), 1.22-1.18 (m,7H), 1.15-1.09 (m, 3H), 1.07-0.94 (m, 3H), 0.72 (s, 3H). ¹⁹F NMR (376MHz, CDCl₃) δ_(F) −111.32. LC- ELSD/MS purity 99%, MS ESI calcd. forC₃₁H₄₄FN₂O₃ [M + H]⁺ 511, found 511. 134 B24 2,4- difluorobenzoic acid

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.16-8.02 (m, 1H), 7.05-6.93 (m, 1H),6.92-6.76 (m, 1H), 6.60-6.43 (m, 1H), 4.27-4.10 (m, 1H), 3.47- 3.27 (m,5H), 2.60 (s, 1H), 1.87-1.62 (m, 7H), 1.58-1.26 (m, 10H), 1.25- 0.91 (m,10H), 0.72 (s, 3H). ¹⁹F NMR (376 MHz, CDCl₃) δ_(F) −104.54, −109.40.LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂F₂NO₃ [M + H]+ 490, found490. SFC 100% de 135

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.17-8.02 (m, 1H), 7.06-6.93 (m, 1H),6.90-6.78 (m, 1H), 6.53-6.35 (m, 1H), 4.29-4.12 (m, 1H), 3.45- 3.33 (m,5H), 2.62 (s, 1H), 1.98-1.90 (m, 1H), 1.89-1.66 (m, 5H), 1.58- 1.30 (m,10H), 1.30-0.98 (m, 11H), 0.75 (s, 3H). ¹⁹F NMR (376 MHz, CDCl₃) δ_(F)−104.74, −109.75. LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂F₂NO₃[M + H]+ 490, found 490. SFC 98% de

Examples 150-153: Synthesis of 1-((R)-1-((3R, 5R, 8R, 9R, 10S, 135, 145,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile (150) &1-((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(151)&5-amino-1-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(152) &5-amino-1-((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(153)

Synthesis of 150.1

To a solution of NaH (8.00 g, 200 mmol, 60% in oil) in DMSO (100 mL) wasadded a solution of trimethylsulfonium iodide (40.7 g, 200 mmol) in THF(100 mL) dropwise at 0° C. After stirring for 30 mins under N₂, 261.1(50 g, 182 mmol) in DMSO (100 mL) was added. After stirring at 25° C.for another 12 h, the mixture was poured into ice-water (w/w=1/1, 400mL), stirred for 20 min, and extracted with EtOAc (3×400 mL). Thecombined organic phase was washed with brine (2×200 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was trituratedwith MeOH (300 mL) at 25° C. and the mother liquid was concentrated togive 150.1 (45 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.67-2.37 (m, 2H), 2.28-2.13 (m, 2H),2.12-2.04 (m, 1H), 1.99-1.89 (m, 1H), 1.88-1.72 (m, 4H), 1.70-1.60 (m,2H), 1.58-1.43 (m, 5H), 1.41-1.04 (m, 8H), 0.92-0.82 (m, 3H)

Synthesis of 150.2

Na (21.5 g, 935 mmol) was added into MeOH (250 mL) at 25° C. inportions. After stirring at 25° C. for 2 h under N₂, 150.1 (45 g, 156mmol) in MeOH (150 mL) was added. After stirring at 75° C. for 12 h, themixture was poured into water (400 mL) and extracted with EtOAc (3×400mL). The combined organic phase was washed with brine (2×200 mL), driedover anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜15% of EtOAc in PE) to give 150.2 (30 g) asoil. The 150.2 (30 g) was purified by flash column [0-5% of EtOAc in PEand DCM (1:1)] to give 150.2 (9 g, 30%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.47-3.31 (m, 5H), 2.42 (dd, J=8.4, 19.2Hz, 1H), 2.12-2.02 (m, 1H), 1.96-1.89 (m, 1H), 1.86-1.67 (m, 6H),1.60-1.41 (m, 7H), 1.38-1.17 (m, 7H), 1.10-1.00 (m, 1H), 0.85 (s, 3H).

Synthesis of 150.3

To a suspension of EtPPh₃Br (30.0 g, 84.0 mmol) in THF (100 mL) wasadded t-BuOK (9.40 g, 84.0 mmol) at 25° C. under N₂. After stirring for1 h, 150.2 (9 g, 28.0 mmol) in THF (50 mL) was added at 25° C. Afterstirring at 40° C. for 3 h, the solution was combined with two batchesprepared from 9 g and 18 g respectively of 150.2. The mixture was pouredinto NH₄Cl (200 mL, aq.) and extracted with EtOAc (3×200 mL). Thecombined organic phase was washed with brine (2×100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was trituratedfrom MeOH (300 mL) and water (300 mL) at 25° C. to give 150.3 (30 g) asoil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.17-5.05 (m, 1H), 3.46-3.32 (m, 5H), 2.59(brs, 1H), 2.43-2.12 (m, 4H), 1.90-1.79 (m, 2H), 1.77-1.69 (m, 7H),1.67-1.61 (m, 4H), 1.43-1.32 (m, 3H), 1.23-1.03 (m, 6H), 0.87 (s, 3H).

Synthesis of 150.4

To a solution of 150.3 (30 g) in THF (150 mL) was added 9-BBN dimer(43.9 g, 180 mmol) at 25° C. After stirring for 1 h, the reactionmixture was sequentially treated with NaOH (108 mL, 5M in water, 541mmol) at 0° C. and hydrogen peroxide (54.1 mL, 541 mmol) dropwise at 0°C. After stirring at 78° C. for 3 h, the reaction mixture was quenchedwith saturated aqueous Na₂S₂O₃ (100 mL) and ice-water (300 mL), stirredfor 20 min and then extracted with EtOAc (3×250 mL). The combinedorganic phase was washed with brine (2×250 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was triturated from CH₃OH(100 mL) and water (500 mL) at 25° C. to give 150.4 (35 g) as oil, whichwas purified by flash column (0˜15% of EtOAc in PE) to give 150.4 (12 g)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.74-3.66 (m, 1H), 3.43-3.36 (m, 5H), 2.60(s, 1H), 1.95-1.71 (m, 6H), 1.64 (s, 4H), 1.60-1.44 (m, 6H), 1.35-1.19(m, 6H), 1.16-1.08 (m, 4H), 0.92-0.80 (m, 1H), 0.75 (s, 1H), 0.65 (s,3H).

Synthesis of 150.5

To a solution of 150.4 (12 g) in DCM (100 mL) was added DMP (33.1 g,78.2 mmol) in one portion at 25° C. After stirring at 35° C. for 30 min,the residue was diluted with NaHCO₃ (100 mL) and filtered. The mixturewas washed with Na₂SO₃ and NaHCO₃ (3:1) in water (100 mL). The aqueousphase was extracted with DCM (100 mL). The combined organic phase waswashed with brine (2×100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give 150.5 (5 g, 42%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 3.45-3.34 (m, 5H), 2.62 (s, 1H), 2.59-2.46 (m,1H), 2.26-2.13 (m, 1H), 2.13-2.09 (m, 1H), 2.11 (s, 3H), 2.03-1.97 (m,1H), 2.03-1.97 (m, 1H), 1.83 (d, J=15.2 Hz, 1H), 1.78-1.63 (m, 5H),1.52-1.33 (m, 5H), 1.31-1.00 (m, 8H), 0.61 (s, 3H).

Synthesis of 150.6

To a solution of 150.5 (2.9 g, 8.3 mmol) in EtOH (30 mL) were added Et₃N(3.5 mL) and hydrazine hydrate (4.16 g, 83.2 mmol) at 25° C. Afterstirring at 75° C. for 12 h, the solution was concentrated. The residuewas diluted with water (30 mL) and extracted with EtOAc (3×20 mL). Thecombined organic layer was washed with brine (30 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 150.6 (3 g, 99%) asan oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.91 (brs, 2H), 3.50-3.30 (m, 5H), 2.60(s, 1H), 2.30-2.10 (m, 2H), 1.90-1.50 (m, 10H), 1.50-0.95 (m, 15H), 0.57(s, 3H).

Synthesis of 150.7

To a solution of 150.6 (3 g, 8.3 mmol) in MeOH (30 mL) were added AcOH(990 mg, 16.5 mmol) and NaBH₃CN (5.20 g, 82.6 mmol) at 25° C. Afterstirring at 75° C. for 12 h, the solution was concentrated. The residuewas diluted with water (30 mL) and extracted with EtOAc (3×20 mL). Thecombined organic layer was washed with brine (30 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 150.7 (3 g, 99%) asoil, which was used directly for the next step without furtherpurification.

Synthesis of 152 & 153

To a solution of 150.7 (3 g, 8.2 mmol) in EtOH (50 mL) were added TEA(2.48 g, 24.6 mmol) and 2-(ethoxymethylidene) propanedinitrile (1.2 g,9.9 mmol) at 25° C. After stirring at 75° C. for 16 h, the mixture wasconcentrated in vacuum and the residue was purified by flash column(20-40% of EtOAc in PE) to give 150.8 (3 g, 82.8%) as solid. 150.8 (500mg, 1.1 mmol) was purified by Prep-HPLC (Column: Agela DuraShell 150mm_25 mm_5 m; Condition: water (0.04% NH₃H₂O+10 mM NH₄HCO₃)-ACN; BeginB: 42; End B: 72; Gradient Time (min): 7.5; 100% B Hold Time (min): 2)to afford 152 (48.8 mg, 10%) as a solid and 153 (200 mg) as solid. 153(200 mg, 0.5 mmol) was further purified by SFC (Column: DAICEL CHIRALPAKAD (250 mm*30 mm, 10 um); Condition: 0.1% NH₃H₂O EtOH; Begin B: 50; EndB: 50) to afford 153 (83.2 mg, 42%) as solid. 152: ¹H NMR (400 MHz,CDCl₃) δ_(H) 7.48 (s, 1H), 4.18 (s, 2H), 4.00-3.80 (m, 1H), 3.50-3.30(m, 5H), 2.55 (s, 1H), 2.18 (q, J=10.0 Hz, 1H), 1.95-1.85 (m, 1H),1.80-1.60 (m, 4H), 1.50-1.15 (m, 11H), 1.10-0.75 (m, 9H), 0.69 (s, 3H),0.46 (d, J=12.4 Hz, 1H). LC-ELSD/MS purity≥99%, 100% de based on H-NMR;MS ESI calcd. for C₂₆H₄₁N₄O₂ [M+H]⁺ 441.3, found 441.3.

153: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.51 (s, 1H), 4.18 (s, 2H), 4.00-3.80(m, 1H), 3.50-3.30 (m, 5H), 2.60 (s, 1H), 2.15-2.00 (m, 1H), 1.95-1.90(m, 1H), 1.85-1.65 (m, 5H), 1.50-0.95 (m, 20H), 0.77 (s, 3H). LC-ELSD/MSpurity≥99%, 100% de based on H-NMR; MS ESI calcd. for C₂₆H₄₁N₄O₂ [M+H]⁺441.3, found 441.3.

Synthesis of 150 & 151

To a solution of 150.8 (1.5 g, 3.4 mmol) in THF (20 mL) was addedtert-butyl nitrite (419 mg, 4.1 mmol) at 25° C. After stirring at 75° C.for 16 h, the mixture was poured into water (50 mL) and extracted withDCM (2×30 mL). The combined organic layers were washed with brine (50mL), dried over anhydrous Na₂SO₄ and concentrated in vacuum to give 150& 151 (1 g) as solid. The diastereomers were purified by prep-HPLC(Column: Agela DuraShell 150 mm_25 mm_5 um; Condition: water (0.04%NH₃H₂O+10 mM NH₄HCO₃)-ACN; Begin B: 55; End B: 85; Gradient Time (min):7.5; 100% B Hold Time (min): 2) to afford 150 & 151 (200 mg, 20%) as asolid. The diastereomers were separated by SFC (Column: DAICEL CHIRALPAKAD-H (250 mm*30 mm, Sum) Condition: 0.1% NH₃H₂O EtOH; Begin B: 40; EndB:40; Gradient Time (min): 7.5; 100% B Hold Time(min): 2) to afford 150(12 mg, 6%, Rt=1.616 min, de=100%) as a solid and 151 (43.7 mg, 22%,Rt=1.867 min, de=99.92%) as a solid.

150: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.78 (s, 1H), 7.75 (s, 1H), 4.40-4.20(m, 1H), 3.50-3.30 (m, 5H), 2.56 (s, 1H), 2.00-1.60 (m, 6H), 1.50-0.95(m, 18H), 0.90-0.60 (m, 5H), 0.20-0.10 (m, 1H).

LC-ELSD/MS purity≥99%, analytic SFC: 100% de; MS ESI calcd. forC₂₆H₄₀N₃O₂ [M+H]⁺ 426.3, found 426.3.

151: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80-7.75 (m, 2H), 4.30-4.10 (m, 1H),3.50-3.30 (m, 5H), 2.61 (s, 1H), 2.00-1.65 (m, 8H), 1.60-1.30 (m, 10H),1.30-0.95 (m, 9H), 0.76 (s, 3H). LC-ELSD/MS purity≥99%, analytic SFC:100% de; MS ESI calcd. for C₂₆H₄₀N₃O₂ [M+H]⁺ 426.3, found 426.3.

Examples 154-157: Synthesis of1-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(156)&1-((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(157)5-amino-1-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(154) &5-amino-1-((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(155)

Synthesis of 154.1

To a solution of B1 (2 g, 6.3 mmol) in ethanol (20 mL) were added Et₃N(3.5 mL) and hydrazine hydrate (3.13 g, 62.6 mmol) at 25° C. Afterstirring at 75° C. for 12 h, the reaction mixture was concentrated,diluted with water (30 mL) and extracted with EtOAc (3×20 mL). Thecombined organic layer was washed with brine (30 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 154.1 (1.8 g, 87%)as oil.

Synthesis of 154.2

To a solution of 154.1 (1.8 g, 5.4 mmol) in MeOH (20 mL) was addedNaBH₃CN (3.39 g, 54.0 mmol) at 25° C. After stirring at 75° C. for 12 h,the solution was concentrated, diluted with water (30 mL) and extractedwith EtOAc (3×20 mL). The combined organic layer was washed with brine(30 mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give154.2 (1.6 g, 89%) as a solid.

Synthesis of 154 & 155

To a solution of 154.2 (1.6 g, 4.8 mmol) in EtOH (20 mL) were added Et₃N(1.98 mL) and 2-(ethoxymethylidene) propanedinitrile (699 mg, 5.73 mmol)at 25° C. After stirring at 85° C. for 16, the solution wasconcentrated, diluted with water (30 mL) and extracted with EtOAc (3×20mL). The combined organic layer was washed with brine (30 mL), driedover anhydrous Na₂SO₄, filtered and concentrated to give 154.3 (1.6 g,81%) as a solid.

154.3 (1.0 g) was purified by pre-HPLC (Column HT C18 Highload 150 mm*25mm*5 m, Condition water (0.04% NH₃H₂O+10 mM NH₄HCO₃)—CH₃CN, Begin B:50%, End B: 80%) to afford 154.3 (200 mg) as a solid.

154.3 (200 mg) was separated by SFC (Column: Waters UPCC with PDADetector) to afford 154 (60 mg, P1, Rt=3.321 min) as a solid and 155 (30mg, P2, Rt=3.669 min) as a solid.

154 (60 mg) was further purified by pre-HPLC (Column Xtimate C18 150*25mm*5 m, Condition: water (10 mM NH₄HCO₃)—CH₃CN, Begin B 52%, End B 82%)to afford 154 (18.1 mg, Rt=3.319 min, de=100%) as a solid.

155 (30 mg) was further purified by pre-HPLC (Column Xtimate C18 150*25mm*5 m, Condition water (10 mM NH₄HCO₃)—CH₃CN, Begin B 52%, End B 82%)to afford 155 (9.2 mg, Rt=3.676 min, de=96.3%) as a solid.

154: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.48 (s, 1H), 4.18 (s, 2H), 3.96-3.85(m, 1H), 2.25-2.15 (m, 1H), 1.95-1.60 (m, 5H), 1.51-1.22 (m, 11H),1.25-0.76 (m, 13H), 0.70 (s, 3H), 0.52-0.45 (m, 1H). LC-ELSD/MS purity99%, analytic SFC: 100% de. MS ESI calcd. for C₂₅H₃₉ N₄₀ [M+H]⁺ 411.3found 411.3.

155: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.51 (s, 1H), 4.20-4.05 (m, 2H),3.95-3.82 (m, 1H), 2.14-2.05 (m, 1H), 2.00-1.75 (m, 5H), 1.75-1.60 (m,2H), 1.52-1.35 (m, 11H), 1.35-1.00 (m, 12H), 0.78 (s, 3H). LC-ELSD/MSpurity 99%, analytic SFC: 96.3% de. MS ESI calcd. for C₂₅H₃₉ N₄O [M+H]⁺411.3 found 411.3.

Synthesis of 156 & 157

To a solution of 154.3 (1 g, 2.4 mmol) in THF (20 mL) was addedtert-butyl nitrite (300 mg, 2.9 mmol) at 25° C. After stirring at 75° C.for 16 h, the mixture was poured into water (50 mL) and extracted withDCM (2×30 mL). The combined organic layers were washed with brine (50mL), dried over anhydrous Na₂SO₄ and concentrated. The product waspurified by flash column (10-30% of EtOAc in PE) at 25° C. to afford154.4 (800 mg) as solid. 154.4 was further purified by pre-HPLC (ColumnHT C18 Highload 150 mm*25 mm*5 m, Condition water (0.04% NH₃H₂O+10 mMNH₄HCO₃)—CH₃CN, Begin B 60, End B 90) to afford 154.4 (200 mg, 25%) as asolid.

The diastereomers were separated by SFC (Column: Chiralpak AD-3 150×4.6mm I.D., 3 m, Mobile phase: 40% of ethanol (0.05% DEA) in CO₂) to afford156 (60 mg, P1, Rt=2.049 min) as a solid and 157 (30 mg, P2, Rt=2.466min) as a solid.

156 (60 mg) was further purified by pre-HPLC (Column Xtimate C18 150*25mm*5 m, Condition water (10 mM NH₄HCO₃)—CH₃CN, Begin B 59%, End B 89%)to afford 156 (19.5 mg, Rt=2.057 min, de=100%) as a solid.

157 (30 mg) was further purified by pre-HPLC (Column Xtimate C18 150*25mm*5 m, Condition water (10 mM NH₄HCO₃)—CH₃CN, Begin B 59%, End B 89%)to afford 157 (13.2 mg, Rt=2.495 min, de=100%) as a solid.

156: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.78 (s, 1H), 7.75 (s, 1H), 4.25-4.35(m, 1H), 2.02-1.88 (m, 2H), 1.81-1.64 (m, 4H), 1.50-1.20 (m, 11H),0.86-0.65 (m, 5H), 0.20-0.12 (m, 1H). LC-ELSD/MS purity 99%, analyticSFC: 100% de. MS ESI calcd. for C₂₅H₃₈N₃O [M+H]⁺ 396.3 found 396.3.

157: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.78-7.74 (m, 2H), 4.24-4.12 (m, 1H),2.01-1.79 (m, 5H), 1.73-1.61 (m, 2H), 1.55-1.24 (m, 10H), 1.29-1.00 (m,14H), 0.77 (s, 3H). LC-ELSD/MS purity 99%, analytic SFC: 100% de. MS ESIcalcd. for C₂₅H₃₈N₃O [M+H]⁺ 396.3 found 396.3.

Examples 158 & 159: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-17-((R)-1-(2H-1,2,3-triazol-2-yl)ethyl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(158) &(3R,5R,8R,9R,10S,13S,14S,17S)-17-((R)-1-(1H-1,2,3-triazol-1-yl)ethyl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(159)

Synthesis of 158.1

To a mixture of EtPPh₃Br (38.2 g, 103 mmol) in THF (100 mL) was addedt-BuOK (11.5 g, 103 mmol) at 25° C. under N₂. After stirring at 40° C.for 30 min, A1 (10 g, 34.4 mmol) was added in portions below 40° C.After stirring at 40° C. for 1 h, the reaction mixture was quenched with10% NH₄Cl aqueous (100 mL) and extracted with EtOAc (2×100 mL). Thecombined organic layers were washed with brine (200 mL), dried overanhydrous Na₂SO₄, filter and concentrated under vacuum to give a solid.The solid was purified by trituration with MeOH/H₂O (1:1, 100 mL) atreflux to give 158.1 (13.7 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.11 (q, J=7.2 Hz, 1H), 2.40-2.30 (m, 1H),2.29-2.17 (m, 2H), 1.90-1.77 (m, 3H), 1.74-1.68 (m, 1H), 1.65 (br d,J=7.2 Hz, 3H), 1.62-1.58 (m, 1H), 1.48-1.35 (m, 6H), 1.35-1.26 (m, 3H),1.26 (s, 3H), 1.24-1.18 (m, 2H), 1.18-1.05 (m, 4H), 0.87 (s, 3H)

Synthesis of 158.2

To a solution of 158.1 (4 g, 13.2 mmol) in THF (40 mL) was added 9-BBNdimer (6.38 g, 26.4 mmol) at 0° C. After stirring at 25° C. for 1 h, thereaction mixture was sequentially treated with ethanol (23 mL) at 25°C., NaOH aqueous (26.4 mL, 5 M, 132 mmol) at 0° C. and H₂O₂ (22.4 g,30%, 198 mmol) dropwise. After stirring at 70° C. for 1 h, the reactionmixture was quenched with saturated aqueous Na₂S₂O₃ (50 mL), stirred at0° C. for 10 minutes, and diluted with water (50 mL). The suspension wasstirred at 25° C. for 1 h, filtered, washed with water (2×40 mL), driedunder vacuum to give 158.2 (3.5 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.79-3.65 (m, 1H), 1.99-1.79 (m, 5H),1.74-1.59 (m, 3H), 1.57-1.52 (m, 2H), 1.50-1.28 (m, 9H), 1.26 (s, 3H),1.22 (d, J=6.4 Hz, 3H), 1.20-0.99 (m, 7H), 0.66 (s, 3H).

Synthesis of 158 & 159

To a solution of 158.2 (1 g, 3.1 mmol) in THF (10 mL) were added2H-1,2,3-triazole (257 mg, 3.7 mmol). triphenylphosphine (1.63 g, 6.2mmol) and diisopropyl azodicarboxylate (1.08 g, 6.2 mmol) at 0° C. Afterstirring at 25° C. for 12 h, the mixture was poured into water (20 mL)and extracted with EtOAc (2×20 mL). The combined organic layer waswashed with brine (2×20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtOAcin PE) to give 158 (80 mg) and 159 (40 mg) both as oil.

158 (80 mg) was further purified by pre-HPLC (Method: SAGE-TJF-019-P1I;Column: Xtimate C18 150*25 mm*5 um; Condition: water (0.225% FA)-ACN;Begin B: 70%; End B: 100%) to afford 158 (34.2 mg, 43%) as a solid.

159 (40 mg) was further purified by pre-HPLC (Method: SAGE-TJF-019-P1H;Column: Xtimate C18 150*25 mm*5 um; Condition: water (0.225% FA)-ACN;Begin B: 90%; Begin B: 100%)) to afford 159 (4.7 mg, 12%) as a solid.

158: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.54 (s, 2H), 4.75-4.60 (m, 1H),2.20-2.08 (m, 1H), 1.99-1.85 (m, 1H), 1.84-1.60 (m, 6H), 1.53-1.50 (m,1H), 1.47 (d, J=6.8 Hz, 3H), 1.45-1.23 (m, 9H), 1.22 (s, 3H), 1.20-0.95(m, 5H), 0.76 (s, 3H), 0.70-0.55 (m, 1H), −0.01- −0.09 (m, 1H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₃H₃₈N₃O [M+H]⁺ 372.3, found372.3.

159: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.71 (d, J=0.8 Hz, 1H), 7.59 (d,J=1.2 Hz, 1H), 5.12-4.96 (m, 1H), 2.42-2.12 (m, 4H), 2.10-2.00 (m, 2H),1.90-1.75 (m, 6H), 1.74-1.59 (m, 5H), 1.52-1.50 (m, 1H), 1.43 (s, 3H),1.40-1.34 (m, 1H), 1.32-1.02 (m, 9H), 0.74 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₃H₃₆N₃[M−H₂O+H]⁺354.3, found 354.3.

Examples 160-163: Synthesis of5-amino-1-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(160) &5-amino-1-((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(161) &1-((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(162) &1-((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(163)

Synthesis of 160.1

To a solution of 206.0 (1.55 g, 4.3 mmol) in ethanol (5 mL) was addedEt₃N (1.8 mL) and hydrazine hydrate (2.50 g, 42.6 mmol) at 25° C. Afterstirring at 80° C. for 12 h, the solution was concentrated. The residuewas diluted with water (50 mL) and extracted with EtOAc (3×30 mL). Thecombined organic layer was washed with brine (30 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 160.1 (1.6 g) asoil. The 160.1 (1.6 g) was purified by flash column (EtOAc in PE=20-50%)to afford 160.1 (1.4 g, 87%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.90 (br, s, 2H), 3.52 (q, J=6.8 Hz, 2H),3.42 (q, J=9.2 Hz, 2H), 2.71 (br, s, 1H), 2.29-2.09 (m, 2H), 1.90-1.55(m, 14H), 1.50-1.25 (m, 7H), 1.25-0.95 (m, 7H), 0.56 (s, 3H).

Synthesis of 160.2

To a solution of 160.1 (1.4 g, 3.7 mmol) in MeOH (5 mL) was added AcOH(445 mg, 7.4 mmol) and NaBH₃CN (2.33 g, 37.1 mmol) at 25° C. Afterstirring at 75° C. for 12 h, the solution was concentrated. The residuewas diluted with water (30 mL) and extracted with EtOAc (3×20 mL). Thecombined organic layer was washed with saturated brine (30 mL), driedover anhydrous Na₂SO₄, filtered and concentrated to give 160.2 (1.2 g,86%) as an oil, which was used to next step directly.

Synthesis of 160 & 161

To a solution of 160.2 (600 mg, 1.6 mmol) in EtOH (5 mL) were added Et₃N(479 mg, 4.7 mmol) and 2-(ethoxymethylidene) propanedinitrile (230 mg,1.9 mmol) at 25° C. After stirring at 75° C. for 16 h, the mixture wasconcentrated in vacuum and the residue was purified by flash column(20-40% of EtOAc in PE) to give a mixture of 160 & 161 (200 mg) as asolid. The diastereomers (200 mg) was purified by pre-HPLC (Column:Waters Xbridge 150*25 mm*5 um; Condition: water (10 Mm NH₄HCO₃)-ACN;Begin B: 50%; End 80%) to give a mixture of 160 & 161 (50 mg) as asolid. The diastereomers (50 mg) were separated by SFC (Column: DAICELCHIRALPAK AD-H (250 mm*30 mm, Sum); Condition: Neu-ETOH; Begin B: 45%;End 45%) to give 160 (8.2 mg, 16%) and 161 (9.8 mg, 19%) as a solid.

160: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.48 (s, 1H), 4.18 (s, 2H), 3.98-3.85(m, 1H), 3.51 (q, J=6.8 Hz, 2H), 3.45-3.35 (m, 2H), 2.67 (s, 1H),2.29-2.09 (m, 1H), 2.00-1.68 (m, 4H), 1.54-1.30 (m, 11H), 1.27-0.78 (m,13H), 0.69 (s, 3H), 0.53-0.40 (m, 1H). LC-ELSD/MS: purity 99%, analyticSFC: 99.26% de; MS ESI calcd. for C₂₇H₄₃N₄O₂ [M+H]⁺ 455.3, found 455.4.

161: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.51 (s, 1H), 4.14 (s, 2H), 3.96-3.80(m, 1H), 3.53 (q, J=7.2 Hz, 2H), 3.42 (q, J=9.6 Hz, 2H), 2.71 (s, 1H),2.13-2.04 (m, 1H), 1.97-1.89 (m, 1H), 1.87-1.61 (m, 6H), 1.48-1.35 (m,10H), 1.26-0.96 (m, 12H), 0.77 (s, 3H). LC-ELSD/MS: purity 99%, analyticSFC: 95.96% de; MS ESI calcd. for C₂₇H₄₃N₄O₂ [M+H]⁺ 455.3, found 455.3.

Synthesis of 162

To a solution of 160 (16 mg, 0.035 mmol) in THF (2 mL) was addedtert-butyl nitrite (4.34 mg, 0.042 mmol) at 15° C. After stirring at 75°C. for 16 h, the mixture was poured into water (10 mL) and extractedwith DCM (2×10 mL). The combined organic layers were washed with brine(30 mL), dried over anhydrous Na₂SO₄ and concentrated in vacuum. Theresidue was purified by flash column (0˜30% of EtOAc in PE) to give 162(15 mg) as oil. 162 (15 mg) was purified again by flash column (0˜30% ofEtOAc in PE) to give 162 (6.1 mg, 41%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.30-4.22 (m,1H), 3.51 (q, J=7.2 Hz, 2H), 3.40 (dd, J=9.2 Hz, 24.8 Hz, 2H), 2.68 (s,1H), 1.95-1.65 (m, 6H), 1.55-1.45 (m, 2H), 1.42 (d, J=6.8 Hz, 3H),1.39-1.22 (m, 7H), 1.22-1.17 (m, 4H), 1.15-0.71 (m, 7H), 0.71 (s, 3H),0.17-0.10 (m, 1H). LC-ELSD/MS: purity 99%, MS ESI calcd. forC₂₇H₄₁N₃O₂[M+H]⁺440.3, found 440.3.

Synthesis of 163

To a solution of 161 (25 mg, 0.055 mmol) in THF (2 mL) was added t-BuONO(6.80 mg, 0.066 mmol) at 15° C. After stirring at 75° C. for 16 h, themixture was poured into water (10 mL) and extracted with DCM (2×10 mL).The combined organic layers were washed with brine (50 mL), dried overanhydrous Na₂SO₄ and concentrated in vacuum. The residue was purified byflash column (0˜20% of EtOAc in PE) to give 163 (2 mg, 8%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.76 (s, 2H), 4.25-4.10 (m, 1H), 3.53 (q,J=7.2 Hz, 2H), 3.42 (dd, J=9.6 Hz, 19.2 Hz, 2H), 2.72 (s, 1H), 1.95-1.60(m, 9H), 1.52-1.50 (m, 3H), 1.49-1.23 (m, 9H), 1.22-1.18 (m, 4H),1.17-1.01 (m, 5H), 0.76 (s, 3H). LC-ELSD/MS: purity 98%, MS ESI calcd.for C₂₇H₄₁N₃O₂[M+H]⁺ 440.3, found 440.3.

Example 164: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-17-((S)-1-(2H-1,2,3-triazol-2-yl)ethyl)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(164)

Synthesis of 164.1

To a solution of B1 (2.0 g, 6.28 mmol) in MeOH (20 mL) was added NaBH₄(355 mg, 9.4 mmol) at 25° C. After stirring at 25° C. for 2 h, themixture was poured into water (200 mL) and extracted with EtOAc (2×100mL). The organic layer was washed with brine (2×100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated in vacuum to give 164.1 (2g, 99%) (including 10% 21-down-Me isomer) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.78-3.67 (m, 1H), 2.12-1.96 (m, 1H),1.90-1.76 (m, 3H), 1.70-1.54 (m, 9H), 1.51-1.30 (m, 8H), 1.22 (m, 2H),1.16-0.99 (m, 9H), 0.75 (s, 3H).

Synthesis of 164

To a solution of 164.1 (500 mg, 1.6 mmol), triphenylphosphane (608 mg,2.3 mmol) and 2H-1,2,3-triazole (138 mg, 2.0 mmol) in THF (10 mL) wasadded DIAD (469 mg, 2.3 mmol) at 0° C. After stirring at 25° C. for 16h, the reaction mixture was concentrated and purified by flash column(0˜45% of EtOAc in PE) to give 164 (20 mg,). 164 (20 mg) was furtherpurified by SFC (Column: DAICEL CHIRALPAK AD-H (250 mm*30 mm, Sum);condition: 0.1% NH₃H₂O ETOH; Begin B: 40%; End B: 40%;) to afford 164(4.3 mg, 22%, P2, Rt=1.926 min) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.55 (s, 2H), 4.68-4.57 (m, 1H), 2.10 (q,J=9.2 Hz, 1H), 1.98 (d, J=12.8 Hz, 1H), 1.92-1.57 (m, 6H), 1.53-1.29 (m,10H), 1.26 (s, 6H), 1.21-0.98 (m, 7H), 0.78 (s, 3H). LCMS: purity 96%,analytic SFC: 99.82% de. MS ESI calcd. for C₂₃H₃₇N₃O [M+H]⁺ 372.3, found372.3.

The following examples were synthesized similar to Examples 25 & 26 withthe listed aniline or to Examples 27 & 28 with the listed amine andappropriate SM. In the case of diasteriomeric products, typically thediastereomeric isomers were separated by SFC (e.g. Column: DAICELCHIRALPAK AS-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃H₂O EtOH, BeginB: 30%, End B 30%) or prep-HPLC (column: DuraShell 150*25 mm*5 um;Condition: water (10 mM NH₄HCO₃)-ACN; 75%-95% in 7 min. FlowRate: 25mL/min) yielding both diastereomers after separation. The diastereomerswere assigned based on 1H NMR of C21-Me.

Example SM Aniline/amine STRUCTURE Analytical 201 B1 3-aminobenzonitrile

¹H NMR (400 MHz, CDCl3) δ_(H) 7.17 (t, J = 8.0 Hz, 1H), 6.88 (d, J = 7.2Hz, 1H), 6.76-6.67 (m, 2H), 3.63 (s, 1H), 3.34 (s, 1H), 1.99-1.78 (m,4H), 1.70-1.58 (m, 3H), 1.47 (d, J = 14.8 Hz, 2H), 1.43-1.29 (m, 9H),1.27 (s, 3H), 1.24- 1.20 (m, 1H), 1.18 (d, J = 6.0 Hz, 3H), 1.16-1.00(m, 6H), 0.75 (s, 3H). LC- ELSD/MS purity 99%, MS ESI calcd. forC₂₈H₄₁N₂O [M + H]⁺ 421, found 421. SFC 100% de. 202

¹H NMR (400 MHz, CDCl3) δ_(H) 7.18 (t, J = 8.0 Hz, 1H), 6.88 (d, J = 7.6Hz, 1H), 6.75-6.65 (m, 2H), 3.61 (s, 1H), 3.36 (s, 1H), 1.99-1.93 (m,1H), 1.90-1.76 (m, 4H), 1.69- 1.54 (m, 5H), 1.50-1.28 (m, 10H), 1.26 (s,3H), 1.22-1.12 (m, 3H), 1.09 (d, J = 6.0 Hz, 3H), 1.06-0.93 (m, 2H),0.63 (s, 3H). LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₁N₂O [M +H]⁺ 421, found 421. SFC 100% de. 203 B1 pyridin-3- amine

¹H NMR (400 MHz, CDCl₃) δ = 7.97-7.92 (m, 1H), 7.91-7.85 (m, 1H),7.09-7.01 (m, 1H), 6.85- 6.73 (m, 1H), 3.44-3.31 (m, 2H), 2.07-1.96 (m,1H), 1.87-1.77 (m, 3H), 1.52-1.34 (m, 8H), 1.32- 1.17 (m, 10H),1.15-0.94 (m, 9H), 0.65 (s, 3H); LCMS purity 99%, MS ESI calcd. forC₂₆H₄₁N₂O [M + H]⁺ 397, found 397; analytical SFC 100% de, 204

¹H NMR (400 MHz, CDCl₃) δ = 7.95 (d, J = 2.8 Hz, 1H), 7.88 (d, J = 3.5Hz, 1H), 7.08-7.01 (m, 1H), 6.85-6.77 (m, 1H), 3.52-3.39 (m, 1H), 3.39-3.28 (m, 1H), 1.99-1.89 (m, 2H), 1.88-1.78 (m, 3H), 1.69-1.66 (m, 1H),1.51-1.46 (m, 1H), 1.46- 1.33 (m, 8H), 1.32-1.25 (m, 7H), 1.24-1.20 (m,1H), 1.20-1.17 (m, 3H), 1.15-1.01 (m, 5H), 0.74 (s, 3H); LCMS purity99%, MS ESI calcd. for C₂₆H₄₁N₂O [M + H]⁺ 397, found 397; analytical SFC97% de, 205 B1 4- aminobenzonitrile

¹H NMR (400 MHz, CDCl₃) δ 7.54-7.30 (m, 2H), 6.51-6.43 (m, 2H),4.01-3.92 (m, 1H), 3.50- 3.38 (m, 1H), 2.08-1.78(m, 3H), 1.78-1.48 (m,9H),1.47-1.33 (m,7H), 1.26-1.16 (m, 7H), 1.15- 1.02 (m, 5 H), 0.74(s,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₁N₂O[M + H]⁺ 421,found 421. SFC 99% de. 206

(1R)-1- phenylethan-1- amine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.27 (m, 4H), 7.24-7.18 (m, 1H),3.92-3.86 (m, 1H), 3.57- 3.50 (m, 2H), 3.48-3.38 (m, 2H), 2.75-2.66 (m,2H), 2.26-2.18 (m, 1H), 1.88-1.71 (m, 4H), 1.69- 1.54 (m, 7H), 1.50-1.31(m, 7H), 1.28 (d, J = 6.8 Hz, 3H), 1.21 (t, J = 7.2 Hz, 4H), 1.13-1.01(m, 5H), 0.89 (d, J = 6.0 Hz, 3H), 0.78 (s, 3H). LC- ELSD/MS purity 99%,MS ESI calcd. for C₃₁H₅₀NO₂ [M + H]⁺ 468, found 468. SFC 100% de. 207

(1R)-1- phenylethan-1- amine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.40-7.28 (m, 4H), 7.25-7.18 (m, 1H),3.95-3.85 (m, 1H), 3.57- 3.48 (m, 2H), 3.47-3.35 (m, 2H), 2.77-2.64 (m,2H), 2.30-2.10 (m 1H), 2.01-1.68 (m, 3H), 1.66- 1.40 (m, 10H), 1.31-1.04(m, 14H), 1.03-1.82 (m, 8H), 0.75 (s, 3H). LC- ELSD/MS purity 99%, MSESI calcd. for C₃₂H₅₂NO₂ [M + H]⁺ 482, found 482. 208 B1 (1R)-1-phenylethan-1- amine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.37-7.27 (m, 4H), 7.24-7.18 (m, 1H), 3.89(m, 1H), 2.81-2.63 (m, 1H), 2.30-2.15 (m, 1H), 1.88 (m, 1H), 1.83- 1.53(m, 6H), 1.52-1.31 (m, 8H), 1.31-1.27 (m, 5H), 1.26 (s, 3H), 1.25- 1.17(m, 3H), 1.15-1.01 (m, 5H), 0.89 (m, 3H), 0.78 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₉H₄₆NO [M + H]⁺ 424, found 424. %. de >99 (by 1H NMR) 209 B1 (1S)-1- phenylethan-1- amine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.27 (m, 4H),7.23 (s, 1H), 3.95- 3.85(m, 1H), 2.29-2.20 (m, 1H), 2.16-2.08 (m, 1H), 1.87-1.75 (m, 1H),1.82-1.48 (m, 9H), 1.48- 1.32 (m, 10H), 1.26 (s, 6H), 1.13-1.02 (m, 4H),0.95 (d, J = 6.0 Hz, 4H), 0.40 (s, 3H).LC- ELSD/MS purity 99%, MS ESIcalcd. for C₂₉H₄₆NO [M + H]⁺ 424, found 424. SFC 100% de. 210

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.27 (m, 4H), 7.20 (s, 1H), 3.78-3.84 (m, 1H), 2.71-2.53 (m, 1H), 2.12-1.72 (m, 5H), 1.69-1.55 (m, 3H),1.41-1.38 (m, 8H), 1.31- 1.22 (m, 10H), 1.10-1.06 (m, 6H), 0.98-0.94 (m,3H), 0.68 (s, 3H).LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₅NO[M + H]⁺ 424, found 424. SFC 99.82% de. 211

(1S)-1- phenylethan-1- amine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.35-7.26 (m, 4H), 7.25-7.19 (m, 1H),3.92-3.85 (m, 1H), 3.56- 3.48 (m, 2H), 3.47-3.36 (m, 2H), 2.71-2.67 (m,1H), 2.30-2.15 (m, 2H), 1.97-1.87 (m, 1H), 1.82- 1.63 (m, 3H), 1.55-1.46(m, 7H), 1.34-1.17 (m, 13H), 1.16-0.98 (m, 4H), 0.98-0.89 (m, 7H), 0.39(s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₂H₅₂NO₂ [M + H]⁺482, found 482. SFC 100% de. 212

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.27 (m, 4H), 7.23-7.17 (m, 1H),3.85-3.78 (m, 1H), 3.57- 3.49 (m, 2H), 3.46-3.36 (m, 2H), 2.68-2.54 (m,2H), 2.05-1.75 (m, 4H), 1.70-1.51 (m, 10H), 1.31- 1.18 (m, 10H),1.17-0.98 (m, 5H), 0.98-0.88 (m, 7H), 0.65 (s, 3H). LC- ELSD/MS purity99%, MS ESI calcd. for C₃₂H₅₂NO₂ [M + H]⁺ 482, found 482. SFC 96.7% de.

Examples 213&214: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17S)-17-((S)-1-(benzylamino)propyl)-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(213)&(3R,5R,8R,9R,10S,13S,14S,17S)-17-((R)-1-(benzylamino)propyl)-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(214)

Synthesis of 213.1

To a suspension of triphenyl(propyl)phosphonium bromide (23.5 g, 61.1mmol) in THF (50 mL) was added t-BuOK (6.84 g, 61.1 mmol) at 25° C.under N₂. After stirring at 60° C. for 1 h, A33 (3.3 g, 10.2 mmol) inTHF (20 mL) was added to the mixture at 60° C. After stirring at 60° C.for 16 h, NH₄Cl (100 mL) was added to the resulting solution. Theorganic layer was separated, dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜5% of EtOAc inPE) to give 213.1 (5.8 g) as an oil.

¹H NMR (400 MHz, CDCl3) S_(H) 5.00 (t, J=6.8 Hz, 1H), 3.42-3.36 (m, 5H),2.57 (s, 1H), 2.39-1.95 (m, 5H), 1.85-1.52 (m, 10H), 1.49-0.99 (m, 10H),0.92 (t, J=7.6 Hz, 3H), 0.86 (s, 3H).

Synthesis of 213.2

To a solution of 213.1 (5.8 g, 16.7 mmol) in anhydrous THF (50 mL) wasadded 9-BBN dimer (10.1 g, 41.7 mmol) under N₂. After stirring at 60° C.for 16 h, the mixture was cooled, quenched by EtOH (30 mL) at 0° C. NaOH(16.6 mL, 5M, 83.4 mmol) was added very slowly. After addition, H₂O₂(16.6 mL, 166 mmol, 10 M) was added slowly until the inner temperatureno longer rises. After stirring at 70° C. for 2 h, the mixture wascooled and poured into water (50 mL) and extracted with EtOAc (3×60 mL).The organic layer was dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (10%˜ 20% ofEtOAc in PE) to give 213.2 (5.8 g, 95%) as an oil. ¹H NMR (400 MHz,CDCl₃) δ_(H)3.53-3.48 (m, 1H), 3.42-3.36 (m, 5H), 2.57 (s, 1H),1.87-1.51 (m, 10H), 1.48-1.30 (m, 8H), 1.27-0.98 (m, 12H), 0.67 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₃H₃₇₀ [M-2H₂O+H]⁺329 found329.

Synthesis of 213.3

To a solution of 213.2 (5.7 g, 15.6 mmol) in DCM (30 mL) was added DMP(13.2 g, 31.2 mmol) at 25° C. After stirring at 25° C. for 1 h, theresulting solution was quenched with NaHCO₃ (50 mL) and extracted withEtOAc (3×30 mL). The combined organic layer was washed with Na₂S₂O₃(3×30 mL, sat.), brine (50 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by column (10% of EtOAc inPE) to give 213.3 (5.83 g) as an oil. ¹H NMR (400 MHz, CDCl₃) δ_(H)3.42-3.36 (m, 5H), 2.60 (s, 1H), 2.52 (t, J=8.8 Hz, 1H), 2.40-1.92 (m,4H), 1.86-1.51 (m, 8H), 1.50-1.30 (m, 7H), 1.29-0.94 (m, 9H), 0.59 (s,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₃H₃₇O₂ [M−H₂O+H]⁺345found 345.

Synthesis of 213 & 214

A mixture of 213.3 (1.5 g, 4.13 mmol), TsOH (39.7 mg, 0.231 mmol) and1-phenylmethanamine (2.2 g, 20.6 mmol) and Ti (OCH₂CH₃)₄ (4.69 g, 20.6mmol) in toluene (20 mL) was stirred at 110° C. for 16 h to givecolorless solution. MeOH (10 mL) was added into the mixture followed byNaBH₄ (1.25 g). After stirring at 25° C. for 2 h, water (20 mL) wasadded to the resulting colorless solution and extracted with EtOAc (3×30mL). The combined organic phase was washed with water (3×30 mL), brine(50 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by column (5% 50% of EtOAc in PE) to give 2.09 g ofdiastereomers, which was separated by SFC (Column: DAICEL CHIRALCEL OJ(250 mm*50 mm, 10 um), Condition: 0.1% NH₃H₂O ETOH, Begin B: 20%, End B:20%) to give 214 (700 mg, Peak 2) and 213 (450 mg, Peak 1) as oils. 213(80 mg, 0.176 mmol) was further purified by SFC (Column: DAICELCHIRALCEL OJ-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃H₂O ETOH, BeginB: 15%, End B: 15%) to give 213 (38 mg, 88%, Rt=2.182 min) as a solid.214 (75 mg, 0.165 mmol) was further purified by SFC (Column: DAICELCHIRALCEL OJ-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃H₂O ETOH, BeginB: 15%, End B: 15%) to give 214 (35 mg, Rt=2.519 min) as a solid.

213: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.29 (m, 4H), 7.24-7.21 (m, 1H),3.78-3.67 (m, 2H), 3.43-3.36 (m, 5H), 2.60 (s, 1H), 2.50-2.46 (m, 1H),1.87-1.50 (m, 11H), 1.49-1.21 (m, 10H), 1.20-0.86 (m, 9H), 0.79 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₈NO₂ [M+H]⁺ 454 found 454.SFC 100% de.

214: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.29 (m, 4H), 7.24-7.21 (m, 1H),3.83-3.77 (m, 1H), 3.62-3.58 (m, 1H), 3.42-3.36 (m, 5H), 2.65-2.55 (m,2H), 2.14-2.06 (m, 1H), 1.86-1.50 (m, 10H), 1.49-1.16 (m, 10H),1.14-0.79 (m, 9H), 0.66 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₃₀H₄₈NO₂ [M+H]⁺ 454 found 454. SFC 96.78% de.

The following examples were synthesized similar to Example 33 with thelisted aryl halide and appropriate SM. In the case of diasteriomericproducts, typically the diastereomeric isomers were separated by SFC(e.g. Column: DAICEL CHIRALPAK AS-H (250 mm*30 mm, 5 um), Condition:0.1% NH₃H₂O EtOH, Begin B: 30%, End B 30%) or prep-HPLC (column:DuraShell 150*25 mm*5 um; Condition: water (10 mM NH₄HCO₃)-ACN; 75%-95%in 7 min. FlowRate: 25 mL/min) yielding both diastereomers afterseparation. The diastereomers were assigned based on 1H NMR of C21-Me.

Example SM Aryl halide STRUCTURE Analytical 250 B18 4-bromo-3- methyl-benzonitrile

¹H NMR (400 MHz, CDCl₃) δ 7.37 (dd, J = 1.8, 8.5 Hz, 1H), 6.54 (d, J =8.5 Hz, 1H), 3.53-3.47 (m, 1H), 2.08 (s, 3H), 1.95-1.56 (m, 8H),1.56-1.15 (m, 16H), 1.15- 0.90 (m, 9H), 0.63 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₉H₄₂N₂O [M + H]⁺ 435, found 435. SFC 99.84% de251

¹HNMR (400 MHz, CDCl3) δ 7.37 (dd, J = 1.9, 8.4 Hz, 1H), 6.54 (d, J =8.5 Hz, 1H), 3.53-3.47 (m, 1H), 2.08 (s, 3H), 2.01-1.56 (m, 6H),1.54-1.15 (m, 17H), 1.15- 1.00 (m, 10H), 0.75 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₉H₄₂N₂O [M + H]⁺ 435, found 435. SFC 99.66% de252 B24 4-bromo-3- methyl- benzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.37 (dd, J = 1.9, 8.5 Hz, 1H), 7.28-7.26(m, 1H), 6.53 (d, J = 8.6 Hz, 1H), 3.50 (br dd, J = 6.2, 10.1 Hz, 1H),3.43- 3.33 (m,5H), 2.08 (s, 3H), 2.03-1.65 (m, 8H), 1.57-1.22 (m, 13H),1.18-0.92 (m, 8H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₃₀H₄₅N₂O₂ [M + H]⁺ 465, found 465. SFC 100% de. 253

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.38-7.33 (m, 1H), 7.29- 7.24 (m, 1H),6.53 (d, J = 8.6 Hz, 1H), 3.58-3.24 (m, 6H), 2.08 (s, 3H), 2.01-1.63 (m,8H), 1.55-1.33 (m, 10H), 1.29-0.98 (m, 11H), 0.74 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. C₃₀H₄₅N₂O₂ [M + H]⁺ 467, found 467. SFC 100%de. 254 B24 3-bromo- benzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.21-7.17 (m, 1H), 6.91- 6.89 (m, 1H),6.76-6.71 (m, 2H), 3.67 (d, J = 9.6 Hz, 1H), 3.45-3.32 (m, 5 H), 2.63(s, 1H), 1.98-1.70 (m, 6H), 1.68-1.53 (m, 5H), 1.54-1.33 (m, 7H),1.30-1.02 (m, 10H), 0.76 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₉H₄₃N₂O₂ [M + H]⁺ 451, found 451. SFC 100% de. 255

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.19-7.15 (m, 1H), 6.88- 6.86 (m, 1H),6.72-6.67 (m, 2H), 3.62 (d, J = 8.4 Hz, 1H), 3.41-3.33 (m, 5 H), 2.62(s, 1H), 1.98-1.70 (m, 6H), 1.68-1.51 (m, 5H), 1.50-1.29 (m, 7H),1.26-0.94 (m, 10H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₉H₄₃N₂O₂ [M + H]⁺ 451, found 451. SFC 100% de. 256 B24 4-bromo-benzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.39 (d, J = 8.8 Hz, 2H), 6.47 (d, J = 8.8Hz, 2H), 3.45- 3.38 (m, 6H), 1.94-1.74 (m, 5H), 1.68-1.59 (m, 5H), 1.49-1.18 (m, 14H), 1.11 (d, J = 6.4 Hz, 3H), 1.05-0.96 (m, 2H), 0.62 (s,3H).LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₃N₂O₂ [M + H]⁺ 451,found 451. SFC 99% de. 257

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.38 (d, J = 8.8 Hz, 2H), 6.49 (d, J = 8.9Hz, 2H), 3.42- 3.38 (m, 6H), 1.97-1.75 (m, 5H), 1.68-1.59 (m, 5H), 1.45-1.32 (m, 10H), 1.19 (d, J = 6.4 Hz, 3H), 1.15-1.03 (m, 6H), 0.74 (s,3H). LC- ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₃N₂O₂ [M + H]⁺ 451,found 451. SFC 99% de 258 E2 5- bromopyridine- 2-carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.97-7.91 (m, 1H), 7.45- 7.38 (m, 1H),6.78-6.70 (m, 1H), 4.15-4.06 (m, 1H), 3.46- 3.31 (m, 6H), 2.66-2.57 (m,1H), 1.97-1.68 (m, 6H), 1.68- 1.61 (m, 5H), 1.39-1.34 (m, 3H), 1.34-1.24(m, 3H), 1.24- 1.18 (m, 4H), 1.18-0.96 (m, 6H), 0.74-0.68 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₂N₃O₂ [M + H]+ 452, found452. 259 F8 1-(3-bromo-2- methylphenyl) pyrrolidin-2- one

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.14-7.05 (m, 1H), 6.58- 6.43 (m, 2H),3.75-3.62 (m, 2H), 3.58-3.33 (m, 6H), 2.75- 2.68 (s, 1H), 2.61-2.53 (m,2H), 2.25-2.08 (s, 3H), 1.95- 1.71 (m, 7H), 1.70-1.59 (m, 4H), 1.50-1.29(m, 8H), 1.26- 1.16 (m, 5H), 1.15-0.90 (m, 8H), 0.68-0.60 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₄H₅₃N₂O₃ [M + H]⁺ 537, found537. 260 F8 4-bromo-3- methyl- benzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.38 (d, J = 8.8 Hz, 1H), 7.30 (s, 1H),6.63 (d, J = 8.8 Hz, 1H), 5.22 (d, J = 9.6 Hz, 1H), 4.13 (s, 1H),3.59-3.50 (m, 2H), 3.47-3.41 (m, 3H), 2.68-2.65 (m, 1H), 2.34-2.31 (m,1H), 2.07 (s, 3H), 1.82- 1.45 (m, 9H), 1.38-1.22 (m, 8H), 1.18-1.14 (m,2H), 1.09 (t, J = 6.8 Hz, 4H), 1.04 (d, J = 6.0 Hz, 3H), 0.98-0.84 (m,2H), 0.54 (s, 3H). LC- ELSD/MS purity 99%, MS ESI calcd. for C₃₁H₄₇N₂O₂[M + H]⁺ 479, found 479. 261 F8 5- bromopyridine- 2-carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.95-7.93 (m, 1H), 7.46-7.42 (m, 1H),6.77-6.72 (m, 1H), 4.07-4.02 (m, 1H), 3.56-3.50 (m, 2H), 3.46-3.38 (m,3H), 2.74 (s, 1H), 1.91-1.73 (m, 4.5H), 1.69-1.56 (m, 4.5H), 1.51- 1.29(m, 9H), 1.27-1.18 (m, 6H), 1.15-1.12 (m, 4H), 1.07-0.97 (m, 2H), 0.62(s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₄N₃O₂ [M + H]⁺466.3 found 466.3. 262 F8 3-bromo-2- fluoropyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.39-7.34 (m, 1H), 7.01- 6.95 (m, 1H),6.92-6.84 (m, 1H), 3.84-3.74 (m, 1H), 3.57- 3.48 (m, 2H), 3.47-3.38 (m,2H), 3.37-3.27 (m, 1H), 2.71 (s, 1H), 2.06-1.99 (m, 1H), 1.93-1.71 (m,4H), 1.69-1.59 (m, 3H), 1.50-1.16 (m, 14H), 1.15-0.92 (m, 8H), 0.63 (s,3H). ¹⁹F NMR (400 MHz, CDCl₃) δ_(F) −87.63. LCMS purity > 99% MS ESIcalcd. for C₂₈H₄₄FN₂O₂ [M + H]⁺ 459, found 459. 263 F8 1-(6-bromopyridin- 2-yl)pyrrolidin- 2-one

¹H NMR (400 MHz, CDCl₃) _(δH) 7.52 (d, J = 8 Hz, 1H), 7.28 (t, J = 8 Hz,1H), 6.04 (d, J = 8 Hz, 1H), 4.11- 3.98 (m, 3H), 3.79-3.69 (m, 1H), 3.53(q, J = 7.2 Hz, 2H), 3.42 (q, J = 8.4 Hz, 2H), 2.71 (s, 1H), 2.61 (t, J= 8.4 Hz, 2H), 2.10-1.96 (m, 3H), 1.89-1.51 (m, 10H), 1.48- 1.16 (m,11H), 1.13-0.91 (m, 8H), 0.64 (s, 3H). LC- ELSD/MS purity 99%, MS ESIcalcd. for C₃₂H₅₀N₃O₃ [M + H]⁺ 524, found 524. 264 F8 1-(5-bromopyridin- 2-yl)-1H- pyrazole-3- carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.44 (s, 1H), 7.76 (d, J = 8.4 Hz, 1H),7.71 (s, 1H), 6.97 (br d, J = 9.6 Hz, 1H), 6.79 (s, 1H), 3.63 (br d, J =9.6 Hz, 1H), 3.57-3.48 (m, 2H), 3.46-3.39 (m, 2H), 2.74 (s, 1H), 1.98(br d, J = 12.5 Hz, 1H), 1.88-1.56 (m, 9H), 1.52-1.30 (m, 9H), 1.29-0.93(m, 12H), 0.65 (s, 3H). LC- ELSD/MS purity 99%, MS ESI calcd. forC₃₂H₄₄N₅O [M − H₂O + H]⁺ 514.3 found 514.3.

Examples 265-268: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17R)-17-((S)-1-(2H-1,2,3-triazol-2-yl)propan-2-yl)-3-(ethoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(265) &(3R,5R,8R,9R,10S,13S,14S,17R)-17-((R)-1-(2H-1,2,3-triazol-2-yl)propan-2-yl)-3-(ethoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(266) &(3R,5R,8R,9R,10S,13S,14S,17R)-17-((S)-1-(1H-1,2,3-triazol-1-yl)propan-2-yl)-3-(ethoxymethyl)-13-methylhexadecahydro-11H-cyclopenta[a]phenanthren-3-ol(267) &(3R,5R,8R,9R,10S,13S,14S,17R)-17-((R)-1-(1H-1,2,3-triazol-1-yl)propan-2-yl)-3-(ethoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(268)

Synthesis of 265.1

To a solution of t-BuOK (833 mg, 7.4 mmol) in THF (15 mL) was addedMePPh₃Br (2.65 g, 7.4 mmol) at 15° C. After stirring at 40° C. for 0.5h, a solution of 265.0 (900 mg, 2.5 mmol) in THF (5 mL) was added intothe reaction. After stirring at 40° C. for 16 h, the resulting mixturewas poured into water (100 mL) and extracted with EtOAc (2×50 mL). Thecombined organic phase was washed with brine (2×100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The product was purified byflash column (0 30% of EtOAc in PE) to give 265.1 (500 mg, 62%) as anoil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 3.53 (q, J=6.8Hz, 2H), 3.45 (q, J=9.2 Hz, 2H), 2.70 (s, 1H), 2.10-1.97 (m, 1H),1.87-1.69 (m, 8H), 1.68-1.61 (m, 3H), 1.57 (s, 5H), 1.46-1.34 (m, 5H),1.31-1.10 (m, 8H), 0.56 (m, 3H).

Synthesis of 265.2

To a solution of 265.1 (500 mg, 1.3 mmol) in THF (5 mL) was addedBH₃.Me₂S (0.414 mL, 10 M, 4.1 mmol) at 15° C. After stirring at 45° C.for 1 h, the resulting mixture was diluted with ethanol (630 mg, 13.7mmol) at 15° C., followed by aqueous NaOH solution (2.74 mL, 5.0 M, 13.7mmol) at 15° C. and H₂O₂ (1.37 mL, 10 M, 13.7 mmol) dropwise at 15° C.After stirring at 80° C. for 1 h, the reaction mixture was cooled to 15°C., poured into water (50 mL) and extracted with EtOAc (2×50 mL). Thecombined organic layer was washed with brine (2×100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 30% of EtOAc in PE) to give 265.2 (500 mg, 95%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.61 (m, 1H), 3.53 (q, J=7.2 Hz, 2H),3.48-3.32 (m, 3H), 2.59-2.26 (m, 1H), 2.00-1.70 (m, 8H), 1.51-1.33 (m,10H), 1.15 (m, 3H), 0.98-0.92 (m, 3H), 0.90-0.76 (m, 8H), 0.68 (s, 3H).

Synthesis of 265.3

To a solution of 265.2 (200 mg, 0.5 mmol) in DCM (10 mL) were added PPh₃(275 mg, 1.0 mmol) and NBS (186 mg, 1.0 mmol) at 0° C. After stirring at25° C. for 1 h, the mixture was poured into water (50 mL) and extractedwith DCM (2×50 mL). The combined organic layer was washed with brine(100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜ 20% of EtOAc in PE) to give265.3 (150 mg, 0.5 mmol, 70%) as a solid.

Synthesis of 265.4 & 265.5

To a solution of 265.3 (150 mg, 0.5 mmol) in DMF (1 mL) were added CsCO₃(220 mg, 0.7 mmol) and 2H-1,2,3-triazole (23.4 mg, 0.2 mmol) at 0° C.After stirring at 85° C. for 12 h, the resulting mixture was cooled to25° C., diluted with water (10 mL) and extracted with EtOAc (3×20 mL).The combined organic phase was washed with water (3×30 mL), brine (30mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give265.4 & 265.5 (120 mg) as an oil. The residue was purified by flashcolumn (0˜ 45% of EtOAc in PE) to give 265.4 (80 mg, 67%, Rf=0.5,PE/EtOAc=1/1) as a solid and 265.5 (40 mg, 34%, Rf=0.4, PE/EtOAc=1/1) asa solid.

Separation of 265 and 266

The 265.4 (80 mg) was separated by SFC ((Column: DAICEL CHIRALCEL OD-H(250 mm*30 mm, 5□m); condition:0.1% NH₃H₂O EtOH; Begin B: 40%; End B:40%;) to afford 265 (5.1 mg, 6%) as a solid and 266 (23 mg, 29%) as asolid.

265: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.59 (s, 2H), 4.47 (dd, J=4.0, 13.6Hz, 1H), 4.12 (dd, J=9.6, 13.2 Hz, 1H), 3.52 (q, J=6.8 Hz, 2H), 3.42 (q,J=9.6 Hz, 2H), 2.69 (s, 1H), 2.20-1.70 (m, 7H), 1.53-1.31 (m, 8H),1.30-1.15 (m, 8H), 1.15-1.00 (m, 5H), 0.81 (d, J=6.4 Hz, 3H), 0.71 (s,3H). LC-ELSD/MS purity 99%, 100% de based on H-NMR. MS ESI calcd. forC₂₆H₄₂N₃O [M−H₂O+H]⁺412.3, found 412.3.

266: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.59 (s, 2H), 4.70 (dd, J=4.4, 13.2Hz, 1H), 4.08 (dd, J=10.8, 12.9 Hz, 1H), 3.53 (q, J=6.8 Hz, 2H), 3.42(q, J=9.2 Hz, 2H), 2.70 (s, 1H), 2.31-2.15 (m, 1H), 1.95-1.61 (m, 8H),1.54-1.31 (m, 8H), 1.30-1.16 (m, 7H), 1.14-0.99 (m, 5H), 0.81 (s, 3H),0.66 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, 100% de based on H-NMR.MS ESI calcd. for C₂₆H₄₂N₃O [M−H₂O+H]⁺412.3, found 412.3.

Separation of 267 and 268

The 265.5 (40 mg) was separated by SFC ((Column: DAICEL CHIRALCEL OD-H(250 mm*30 mm, 5□m); condition:0.1% NH₃H₂O EtOH; Begin B: 40%; End B:40%;) to afford 267 (7.6 mg, 19%) as a solid and 268 (15 mg, 38%) as asolid.

267: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70 (s, 1H), 7.50 (s, 1H), 4.44 (dd,J=3.6, 13.8 Hz, 1H), 4.03 (dd, J=9.6, 13.6 Hz, 1H), 3.52 (q, J=6.8 Hz,2H), 3.42 (q, J=9.2 Hz, 2H), 2.71 (s, 1H), 2.09-1.68 (m, 7H), 1.51-1.28(m, 9H), 1.23-0.99 (m, 12H), 0.84 (d, J=6.4 Hz, 3H), 0.71 (s, 3H).LC-ELSD/MS purity 99%, 100% de based on H-NMR. MS ESI calcd. C₂₆H₄₄N₃O₂[M+H]⁺ 430.3, found 430.3.

268: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70 (s, 1H), 7.50 (s, 1H), 4.68 (d,J=10.8 Hz, 1H), 4.05-3.83 (m, 1H), 3.54-3.52 (m, 2H), 3.46-3.40 (q,J=9.2 Hz), 2.72 (s, 1H), 1.97-1.73 (m, 5H), 1.53-1.32 (m, 9H), 1.31-1.17(m, 8H), 1.16-0.96 (m, 6H), 0.81 (s, 3H), 0.70 (d, J=6.4 Hz, 3H).LC-ELSD/MS purity 99%, 100% de based on H-NMR. MS ESI calcd. forC₂₆H₄₄N₃O₂ [M+H]⁺ 430.3, found 430.3.

Examples 269 & 270: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(269) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(270)

To a solution of 265.3 (150 mg, 0.5 mmol) in DMF (1 mL) were added CsCO₃(220 mg, 0.7 mmol) and 1H-pyrazole-4-carbonitrile (47.2 mg, 0.5 mmol) at0° C. After stirring at 85° C. for 12 h, the reaction mixture wasdiluted with water (10 mL) and extracted with EtOAc (3×20 mL). Thecombined organic phase was washed with water (3×30 mL), brine (30 mL),dried over anhydrous Na₂SO₄, filtered and concentrated to give 269.1(153 mg) as an oil. The residue was purified by flash column (0˜ 50% ofEtOAc in PE) and then by pre-HPLC (Column: Waters Xbridge 150*25 5u);condition:water (10 mM NH₄HCO₃)-ACN; Begin B: 70%; End B: 100%;) toafford 269.1 (50 mg, 50%) as a solid. The diastereomers were separatedby SFC (Column: DAICEL CHIRALPAK AS-H (250 mm*30 mm, 5□m); condition:0.1% NH₃H₂O EtOH; Begin B: 30%; End B: 30%;) to afford 269 (13.9 mg,22%) and 270 (12.7 mg, 34%) as solids.

269: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.50 (dd,J=4.4, 13.2 Hz, 1H), 3.70-3.60 (m, 1H), 3.53 (q, J=7.2 Hz, 2H), 3.43 (q,J=9.2 Hz, 2H), 2.72 (s, 1H), 2.20-2.02 (m, 1H), 1.93-1.63 (m, 7H),1.53-1.29 (m, 8H), 1.27-1.00 (m, 12H), 0.79 (s, 3H), 0.67 (d, J=6.4 Hz,3H). LC-ELSD/MS purity 99%, analytic SFC: 100% de. MS ESI calcd. forC₂₈H₄₂N₃O [M−H₂O+H]⁺436.3, found 436.3.

270: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.26 (dd,J=3.2, 13.6 Hz, 1H), 3.80-3.63 (m, 1H), 3.52 (q, J=6.8 Hz, 2H), 3.41 (q,J=9.2 Hz, 2H), 2.71 (s, 1H), 2.10-1.62 (m, 8H), 1.52-1.29 (m, 8H),1.27-0.96 (m, 12H), 0.80 (d, J=6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MSpurity 99%, analytic SFC: 92.94% de. MS ESI calcd. for C₂₈H₄₂N₃O[M−H₂O+H]⁺436.3, found 436.3.

Examples 271-274: Synthesis of1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(271) &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(272) &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(273) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(274)

Synthesis of 271.2

To a solution of 271.1 (3.5 g, 11.4 mmol) in DCM (50 mL) was added PCC(4.9 g, 22.8 mmol) at 25° C. After stirring at 25° C. for 2 h, theresulting mixture was filtered, and the filter cake was washed with DCM(2×50 mL). The combined filtrate was concentrated. The residue waspurified by silica gel column eluted with PE/EtOAc=6/1 to afford 271.2(2.8 g, 77%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 2.65-2.50 (m, 2H), 2.25-2.01 (m, 9H),1.80-1.66 (m, 4H), 1.65-1.55 (m, 2H), 1.54-1.44 (m, 4H), 1.43-1.31 (m,1H), 1.30-1.11 (m, 5H), 0.65 (s, 3H).

Synthesis of 271.3

To a solution of BHT (12 g, 54.4 mmol) in toluene (120 mL) under N₂ at0° C. was added trimethylaluminum (2 M in toluene, 14 mL, 28 mmol)dropwise. After stirring at 25° C. for 1 h, a solution of 271.2 (6 g,19.8 mmol) in DCM (60 mL) was added dropwise at −70° C. After stirringat −70° C. for 1 h under N₂, EtMgBr (20 mL, 60 mmol, 3M in ethyl ether)was added dropwise at −70° C. After stirring at −70° C. for another 1 h,the reaction mixture was poured into citric acid (600 mL, sat.) at 10°C. and extracted with DCM (2×800 mL). The combined organic layer wasdried over anhydrous Na₂SO₄, filtered and concentrated. The residue wastriturated by PE to give 271.3 (3.83 g, 58%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.56-2.50 (m, 1H), 2.24-2.10 (m, 4H),2.07-1.99 (m, 1H), 1.89-1.51 (m, 9H), 1.50-1.20 (m, 12H), 1.19-1.00 (m,3H), 0.98-0.80 (m, 3H), 0.61 (s, 3H).

Synthesis of 271.4

To a suspension of MePh₃PBr (6.4 g, 18.0 mmol) in THF (50 mL) was addedt-BuOK (2.01 g, 18.0 mmol). After stirring at 40° C. for 10 mins, themixture was slowly added dropwise to a solution of 271.3 (3 g, 9.02mmol) in THF (30 mL) at 20° C. for 30 mins. After the addition, themixture was quenched with sat. NH₄Cl (100 mL) and extracted with EtOAc(3×100 mL). The combined organic phase was washed with sat. NH₄Cl (100mL), dried over anhydrous Na₂SO₄, filtered, concentrated and purified byflash column (0˜ 25% of EtOAc in PE) to give 271.4 (2.445 g, 82%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H), 2.02-1.53 (m,13H), 1.50-1.33 (m, 4H), 1.32-1.11 (m, 11H), 1.10-0.99 (m, 2H),0.85-0.80 (m, 3H), 0.56 (s, 3H).

Synthesis of 271.5

To a solution of 271.4 (2.44 g, 7.38 mmol) in THF (20 mL) was addedBH₃/Me₂S (2.8 g, 10 M, 36.9 mmol) dropwise at 25° C. under N₂. Afterstirring at 25° C. for 2 h, the mixture was cooled and quenchedsequentially with EtOH (3.39 g, 73.8 mmol) at 0° C., NaOH (14.7 mL, 5 M,73.8 mmol) slowly and finally H₂O₂ (7.38 mL, 10 M, 73.8 mmol) dropwiseuntil the reaction temperature no longer rises. After the reactiontemperature remained below 0° C., the mixture was poured into water (30mL), stirred for 30 mins. and extracted with EtOAc (2×30 mL). Thecombined organic solution was washed with water (2×30 mL), dried overanhydrous Na₂SO₄, filtered, concentrated and purified by flash column(0˜ 30% of EtOAc in PE) to give 271.5 (2.257 g, 87%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.76-3.71 (m, 1H), 3.47-3.36 (m, 1H),1.98-1.74 (m, 5H), 1.73-1.50 (m, 5H), 1.49-1.32 (m, 5H), 1.31-1.20 (m,9H), 1.16-1.00 (m, 5H), 0.98-0.79 (m, 6H), 0.67 (s, 3H).

Synthesis of 271.6

To a solution of 271.5 (1.2 g, 3.44 mmol) in DCM (10 mL) at 0° C. wereadded PPh₃ (1.35 g, 5.15 mmol) and NBS (916 mg, 5.15 mmol). Afterstirring at 25° C. for 2 h, the reaction was diluted with water (10 mL)and extracted with DCM (2×15 mL). The combined organic phase was washedwith brine (2×15 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 10% of EtOAcin PE) to give 271.6 (1.006 g, 71%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.61-3.48 (m, 1H), δ 3.39-3.34 (m, 1H),2.03-1.83 (m, 2H), 1.82-1.60 (m, 6H), 1.59-1.51 (m, 6H), 1.50-120 (m,13H), 1.19-0.91 (m, 4H), 0.90-0.78 (m, 3H), 0.67 (s, 3H).

Synthesis of 271.7 & 271.8

To a solution of 271.6 (1 g, 2.43 mmol) in DMF (10 mL) was added Cs₂CO₃(1.57 g, 4.86 mmol) and 1H-pyrazole-3-carbonitrile (452 mg, 4.86 mmol)at 25° C. After stirring at 85° C. for 12 h, the reaction mixture wasdiluted with water (20 mL) and extracted with EtOAc (3×20 mL). Thecombined organic phase was washed with water (30 mL) and aq. LiCl (30mL, 5%), brine (30 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 30% of EtOAcin PE) to give 271.7 (544 mg, 53%) and 271.8 (163 mg, 16%) both as oils.

271.7: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70-7.68 (m, 1H), 6.77-6.75 (m,1H), 4.61-4.33 (m, 1H), 3.99-3.77 (m, 1H), 2.26-2.09 (m, 4H), 2.08-1.78(m, 4H), 1.77-1.56 (m, 5H), 1.50-1.25 (m, 8H), 1.24-1.20 (m, 5H),1.19-1.00 (m, 5H), 0.99-0.75 (m, 3H), 0.74-0.65 (m, 3H).

271.8: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.68-7.67 (m, 1H), 7.40-7.26 (m,1H), 6.77-6.64 (m, 1H), 4.53-4.24 (m, 1H), 3.78-3.66 (m, 1H), 2.24-2.11(m, 1H), 2.00-1.74 (m, 6H), 1.73-1.56 (m, 6H), 1.50-1.25 (m, 9H),1.24-1.09 (m, 5H), 0.99-0.80 (m, 3H), 0.79-0.76 (m, 3H), 0.74-0.65 (m,3H).

Separation of 271 & 272

271.7 (544 mg, 1.28 mmol) was separated by SFC (column: DAICEL CHIRALCELOJ-H (250 mm*30 mm, Sum), condition: 0.1% NH₃H₂O ETOH, Begin B: 20%, EndB: 20%, FlowRate (ml/min): 60, Injections: 80) to give 271 (97.6 mg,18%) and 272 (182.9 mg, 33%) as a solid. The two diastereomers wereassigned based on ¹H NMR of C21-Me (C21-down-Me is at more downfieldthan C21-up isomer).

271: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.40-7.39 (m, 1H), 6.66-6.64 (m, 1H),4.30-4.24 (m, 1H), 3.78-3.71 (m, 1H), 1.93-1.74 (m, 3H), 1.80-1.68 (m,3H), 1.67-1.52 (m, 7H), 1.50-1.25 (m, 7H), 1.24-1.00 (m, 8H), 0.99-0.80(m, 3H), 0.78-0.75 (m, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₇H₄₂N₃O [M+H]⁺ 424, found 424. SFC 100% de.

272: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.40-7.38 (m, 1H), 6.66-6.64 (m, 1H),4.53-4.47 (m, 1H), 3.73-3.66 (m, 1H), 2.17-2.10 (m, 1H), 1.90-1.68 (m,5H), 1.67-1.51 (m, 5H), 1.50-1.25 (m, 11H), 1.24-1.20 (m, 2H), 1.19-1.00(m, 4H), 0.90-0.80 (m, 3H), 0.79 (s, 3H), 0.69-0.66 (m, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₇H₄₂N₃O [M+H]⁺ 424, found 424. SFC99.84% de.

Separation of 273 & 274

271.8 (163 mg, 0.384 mmol) was separated by SFC (column: DAICELCHIRALPAK AD-H (250 mm*30 mm, Sum), condition: 0.1% NH₃H₂O IPA, Begin B:40%, End B: 40%, FlowRate (ml/min):50, Injections: 100) to give 273 (70mg) as a solid and 274 (53 mg) as a solid. The 273 (70 mg) wasre-purified by SFC (column: DAICEL CHIRALCEL OJ-H (250 mm*30 mm, Sum),condition: 0.1% NH₃H₂O ETOH, Begin B: 15%, End B: 15%; FlowRate(ml/min):60) to give 273 (21.8 mg) as a solid. The 274 (53 mg) wasre-purified by SFC (column: DAICEL CHIRALCEL OJ-H (250 mm*30 mm, Sum),condition: 0.1% NH₃H₂O ETOH, Begin B: 15%, End B: 15%, FlowRate(ml/min):60, Injections: 45) to give 274 (19.8 mg) as a solid. The twodiastereomers were assigned based on ¹H NMR of C21-Me (C21-down-Me is atmore downfield than C21-up isomer).

273: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.57-7.56 (m, 1H), 6.78-6.77 (m, 1H),4.63-4.57 (m, 1H), 3.94-3.87 (m, 1H), 2.22-2.18 (m, 1H), 1.90-1.81 (m,2H), 1.80-1.63 (m, 3H), 1.62-1.58 (m, 5H), 1.50-1.48 (m, 2H), 1.47-1.20(m, 10H), 1.19-1.00 (m, 5H), 0.95-0.78 (m, 6H), 0.70-0.67 (m, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₂N₃O [M+H]⁺ 424, found424. SFC 100% de.

274: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58-7.56 (m, 1H), 6.78-6.76 (m, 1H),4.40-4.35 (m, 1H), 3.95-3.88 (m, 1H), 2.11-1.97 (m, 1H), 1.94-1.77 (m,2H), 1.75-1.63 (m, 3H), 1.62-1.58 (m, 5H), 1.50-1.48 (m, 3H), 1.47-1.20(m, 9H), 1.19-1.00 (m, 5H), 0.97-0.76 (m, 6H), 0.72 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₇H₄₂N₃O [M+H]⁺ 424, found 424. SFC 100%de.

Examples 275-276: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(275) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(276)

Synthesis of 275.1

To a solution of 271.6 (333 mg, 0.81 mmol) in DMF (5 mL) was addedCs₂CO₃ (523 mg, 1.61 mmol) and 1H-pyrazole-3-carbonitrile (82.7 mg, 0.89mmol) at 25° C. After stirring at 85° C. for 12 h, the resultingcolorless solution was diluted with EtOAc (50 mL) and washed with water(50 mL), brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 20% EtOAc inPE) to give 275.1 (200 mg, 58%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80-7.75 (m, 2H), 4.52-4.23 (m, 1H),3.76-3.62 (m, 1H), 2.10-2.04 (m, 1H), 2.03-1.73 (m, 5H), 1.72-1.50 (m,5H), 1.49-1.23 (m, 8H), 1.22-1.00 (m, 8H), 0.95-0.83 (m, 4H), 0.82-0.76(m, 3H), 0.75-0.67 (m, 3H).

Separation of 275 & 276

275.1 (200 mg) was separated by SFC (DAICEL CHIRALCEL OD-H (250 mm*30mm, 5 um), Condition: 0.1% NH₃H₂O ETOH Begin B: 35% End B: 35% FlowRate(ml/min): 50) to give 276 (68 mg, 34%, Peak 2, Rt=1.835 min) and 275 (53mg, 27%, Peak 1, Rt=1.529 min) as solids. The two diastereomers wereassigned based on ¹H NMR of C21-Me (C21-down-Me is at more downfieldthan C21-up isomer).

275: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80-7.75 (m, 2H), 4.28-4.23 (m, 1H),3.76-3.69 (m, 1H), 2.05-1.92 (m, 3H), 1.90-1.75 (m, 3H), 1.74-1.55 (m,5H), 1.54-1.24 (m, 10H), 1.23-0.98 (m, 7H), 0.95-0.83 (m, 3H), 0.82-0.81(m, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, analytic SFC: 99.82% de;MS ESI calcd. for C₂₇H₄₀N₃[M+H—H₂O]⁺406, found 406; C₂₇H₄₁N₃ONa[M+Na]⁺446.3, found 446.3.

276: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80-7.75 (m, 2H), 4.52-4.47 (m, 1H),3.69-3.62 (m, 1H), 2.13-2.07 (m, 1H), 1.90-1.75 (m, 5H), 1.74-1.55 (m,5H), 1.54-1.48 (m, 3H), 1.47-1.25 (m, 6H), 1.24-1.23 (m, 3H), 1.22-1.00(m, 5H), 0.95-0.80 (m, 3H), 0.79 (s, 3H), 0.69-0.67 (m, 3H). LC-ELSD/MSpurity 99%, analytic SFC: 99.70% de; MS ESI calcd. for C₂₇H₄₀N₃[M+H—H₂O]⁺406, found 406; C₂₇H₄₁N₃ONa [M+Na]⁺446.3, found 446.3.

Examples 278-281: Synthesis of1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(278) &1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(279) &1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(280) &1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(281)

Synthesis of 278.1

To a solution of MePPh₃Br (2.59 g, 7.95 mmol) in THF (30 mL) was addedt-BuOK (892 mg, 7.95 mmol) at 25° C. After stirring at 25° C. for 1 h,207.1 (1 g, 2.65 mmol) in THF (10 mL) was added at 25° C. After stirringat 50° C. for 3 h, the mixture was treated with NH₄Cl (20 mL, sat) andextracted with EtOAc (2×30 mL). The combined organic phase was washedwith brine (10 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was triturated from MeOH (30 mL) and water (30mL) at 25° C. to give 278.1 (900 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.88-4.65 (m, 2H), 3.59-3.34 (m, 4H),2.10-1.96 (m, 2H), 1.85-1.63 (m, 9H), 1.52-1.34 (m, 6H), 1.29-1.17 (m,9H), 1.02-0.89 (m, 7H), 0.63-0.50 (m, 3H).

Synthesis of 278.2

To a solution of 278.1 (900 mg, 2.40 mmol) in THF (20 mL) was addedBH₃.Me₂S (2.15 mL, 21.5 mmol) at 25° C. After stirring for 1 h, thereaction was sequentially treated with NaOH (1.91 g, 47.9 mmol in water)at 0° C. and H₂O₂ (4.79 mL, 10 M in water, 47.9 mmol) also at 0° C.After stirring at 70° C. for 1 h, the resulting colourless solution wascooled to 25° C., poured into water (50 mL) and extracted with EtOAc(3×30 mL). The combined organic phase was washed with brine (2×20 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜ 30% of EtOAc in PE) to give 278.2 (380 mg,40%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.78-3.61 (m, 1H), 3.58-3.31 (m, 5H),2.00-1.77 (m, 5H), 1.72-1.67 (m, 1H), 1.62-1.47 (m, 5H), 1.44-1.34 (m,5H), 1.32-1.17 (m, 9H), 1.12-1.00 (m, 4H), 0.97-0.88 (m, 6H), 0.66 (s,3H)

Synthesis of 278.3

To a solution of 278.2 (330 mg, 0.8404 mmol) in DCM (10 mL) at 0° C. wasadded PPh₃ (262 mg, 1.00 mmol) and NBS (177 mg, 1.00 mmol). Afterstirring at 25° C. for 3 h, the resulting solution was poured into water(50 mL) and extracted with DCM (3×50 mL). The combined organic phase waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 15% of EtOAcin PE) to give 278.3 (309 mg, 81%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.63 (br d, J=9.6 Hz, 0.5H), 3.57-3.26 (m,5.5H), 2.68 (s, 1H), 1.94-1.65 (m, 6H), 1.50 (br d, J=12.0 Hz, 2H),1.41-1.15 (m, 17H), 1.08 (br d, J=5.2 Hz, 4H), 0.98-0.91 (m, 4H), 0.66(s, 3H)

Synthesis of 278.4 & 278.5

To a solution of 278.3 (309 mg, 0.6805 mmol) and Cs₂CO₃ (443 mg, 1.36mmol) in DMF (3 mL) was added 1H-pyrazole-3-carbonitrile (126 mg, 1.36mmol) at 25° C. under N₂. After stirring at 80° C. for 16 h, theresulting solution was cooled to 25° C., poured into water (100 mL) andextracted with EtOAc (3×100 mL). The combined organic phase was washedwith brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give 278.4 (180 mg, 57%) and 278.5 (90 mg, 28%) both as oils.

Separation of 278 and 279

278.4 was purified by SFC (column: DAICEL CHIRALCEL OD-H (250 mm*30 mm,5 um), gradient: 30% condition: 0.1% NH₃H₂O ETOH, flow rate: 60 mL/min)to give 278 (23 mg, Peak 1, Rt=1.618 min) and 279 (30 mg, Peak 2,Rt=2.275 min) both as solids.

278: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.39 (d, J=2.0 Hz, 1H), 6.65 (d,J=2.4 Hz, 1H), 4.28 (dd, J=3.6, 13.6 Hz, 1H), 3.72 (dd, J=10.0, 13.2 Hz,1H), 3.59-3.31 (m, 4H), 2.70 (s, 1H), 2.09-1.98 (m, 1H), 1.97-1.76 (m,4H), 1.74-1.58 (m, 3H), 1.53-1.30 (m, 10H), 1.23-1.08 (m, 8H), 1.02-0.90(m, 4H), 0.79 (d, J=6.4 Hz, 3H), 0.69 (s, 3H). LC-ELSD/MS purity 99%, MSESI calcd. for C₂₉H₄₄N₃O [M+H—H₂O]⁺450, found 450; C₂₉H₄₅N₃O₂Na[M+Na]⁺490, found 490. SFC 100% de.

279: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.39 (d, J=2.4 Hz, 1H), 6.65 (d,J=2.4 Hz, 1H), 4.49 (dd, J=4.8, 13.2 Hz, 1H), 3.68 (dd, J=10.8, 13.2 Hz,1H), 3.58-3.36 (m, 4H), 2.71 (s, 1H), 2.19-2.05 (m, 1H), 2.00-1.77 (m,4H), 1.70 (br d, J=14.4 Hz, 1H), 1.64-1.55 (m, 4H), 1.47-1.33 (m, 6H),1.30-1.08 (m, 10H), 1.03-0.92 (m, 4H), 0.77 (s, 3H), 0.67 (d, J=6.4 Hz,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₄N₃O [M+H—H₂O]⁺450,found 450; C₂₉H₄₅N₃O₂Na [M+Na]⁺490, found 490. SFC 100% de.

Separation of 280 and 281

278.5 was purified by SFC (column: DAICEL CHIRALPAK AD-H (250 mm*30 mm,5 um), gradient: 25% condition: 0.1% NH₃H₂O ETOH, flow rate: 50 mL/min)to give 280 (5 mg, Peak 1, Rt=1.036 min) and 281 (1.6 mg, Rt=1.223 min)both as solids.

280: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.56 (d, J=2.4 Hz, 1H), 6.77 (d,J=2.4 Hz, 1H), 4.58 (dd, J=4.8, 13.2 Hz, 1H), 3.90 (dd, J=10.8, 13.2 Hz,2H), 3.59-3.35 (m, 5H), 2.70 (s, 1H), 2.19 (br s, 2H), 2.00-1.76 (m,5H), 1.70 (br d, J=14.8 Hz, 4H), 1.48-1.31 (m, 7H), 1.28-1.08 (m, 6H),1.03-0.91 (m, 4H), 0.80 (s, 3H), 0.69 (d, J=6.8 Hz, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₉H₄₆N₃O₂ [M+H]⁺ 468, found 468;C₂₉H₄₅N₃O [M+H—H₂O]⁺450, found 450; C₂₉H₄₅N₃O₂Na [M+Na]⁺490, found 490.SFC 100% de.

281: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.57 (d, J=2.0 Hz, 1H), 6.77 (d,J=2.0 Hz, 1H), 4.37 (dd, J=3.6, 13.2 Hz, 1H), 3.91 (dd, J=10.4, 13.8 Hz,1H), 3.58-3.34 (m, 5H), 2.69 (s, 1H), 2.11 (br d, J=10.8 Hz, 1H),2.00-1.76 (m, 5H), 1.70 (br d, J=14.8 Hz, 3H), 1.50-1.31 (m, 7H),1.29-1.07 (m, 9H), 1.03-0.89 (m, 4H), 0.81 (d, J=6.5 Hz, 3H), 0.70 (s,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₄N₃O [M+H—H₂O]⁺450,found 450. SFC 100% de.

Examples 282 & 283: Synthesis of1-((S)-2-((3R,5R,8S,9S,10S,11R,13S,14S,17R)-3,11-dihydroxy-3-(methoxymethyl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(282) &1-((R)-2-((3R,5R,8S,9S,10S,11R,13S,14S,17R)-3,11-dihydroxy-3-(methoxymethyl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(283)

Synthesis of 282.2

To a solution of Me₃SI (7.95 g, 39.0 mmol) in DMSO (30 mL) was addedsodium hydride (935 mg, 39.0 mmol). After stirring at 0° C. for 30 minunder N₂, a solution of 282.1 (5 g, 15.0 mmol) in DMSO (20 mL) wasadded. After stirring at 25° C. for 3 h under N₂, the mixture wasquenched with NH₄Cl (100 mL, sat.) and extracted with EtOAc (2×75 mL).The combined organic layers were washed with brine (150 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 282.2 (5 g, 96.3%)as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.02-3.85 (m, 1H), 2.78-2.50 (m, 4H),2.48-2.18 (m, 3H), 2.17-2.13 (m, 1H), 2.12 (d, J=2.4 Hz, 3H), 1.90-1.59(m, 6H), 1.58-1.27 (m, 8H), 1.20-1.13 (m, 2H), 1.12-1.07 (m, 3H), 0.97(s, 3H), 0.62 (d, J=10.0 Hz, 3H).

Synthesis of 282.3

To a solution of 282.2 (11 g, 31.7 mmol) in MeOH (100 mL) was addedMeONa (8.53 g, 158 mmol) at 25° C. After stirring at 60° C. for 16 h,the mixture was added quenched with NH₄Cl (60 mL, sat) and extractedwith DCM (3×30 mL). The combined organic phase was washed with brine(2×30 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (20˜ 40% of EtOAc in PE) to give282.3 (3.3 g, 27.4%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.90 (td, J=10.4, 15.6 Hz, 1H), 3.43-3.33(m, 6H), 3.16 (s, 1H), 2.80-2.62 (m, 1H), 2.60-2.45 (m, 2H), 2.33-2.27(m, 1H), 2.23-2.15 (m, 1H), 2.12 (s, 3H), 2.08-2.03 (m, 1H), 1.93-1.83(m, 1H), 1.82-1.75 (m, 1H), 1.74-1.58 (m, 8H), 1.57-1.33 (m, 7H),1.21-1.12 (m, 4H), 1.05 (s, 3H), 1.01-0.86 (m, 4H), 0.84-0.68 (m, 1H),0.60 (d, J=2.0 Hz, 3H).

Synthesis of 282.4

To a mixture of MePPh₃Br (9.32 g, 26.1 mmol) in THF (80 mL) was addedt-BuOK (2.92 g, 26.1 mmol) at 15° C. under N₂. After stirring at 15° C.for 30 mins, 282.3 (3.3 g, 8.71 mmol) in THF (20 mL) was added. Afterstirring at 40° C. for 2 h, the resulting suspension was poured intoNH₄Cl (150 mL) and extracted with EtOAc (2×100 mL). The combined organicphase was washed with brine (20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was refluxed in MeOH (80 mL) at70° C. for 30 mins, cooled to 15° C., added water (80 mL), filtered andconcentrated to give 282.4 (1.6 g, 48.7%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.85 (s, 1H), 4.70 (s, 1H), 3.95-3.80 (m,1H), 3.39 (s, 5H), 3.21-3.12 (m, 1H), 2.66-2.58 (m, 1H), 2.55-2.46 (m,1H), 2.26-2.06 (m, 3H), 1.98-1.80 (m, 2H), 1.75 (s, 3H), 1.72-1.66 (m,4H), 1.57-1.49 (m, 1H), 1.47-1.33 (m, 4H), 1.32-1.28 (m, 1H), 1.21-1.09(m, 4H), 1.06 (s, 2H), 1.01-0.82 (m, 4H), 0.56 (d, J=3.2 Hz, 3H).

Synthesis of 282.5

To a solution of 282.4 (600 mg, 1.59 mmol) in THF (10 mL) was addedBH₃.Me₂S (0.477 mL, 4.77 mmol, 10 M) at 0° C. After stirring at 20° C.for 12 h, the resulting mixture was sequentially treated with ethanol(10 mL), NaOH aqueous (3.18 mL, 5.0 M) at 0° C. and then hydrogenperoxide (1.90 mL, 10 M) dropwise at 0° C. After the addition wascomplete, the reaction mixture was quenched with saturated aqueousNa₂S₂O₃ (30 mL) and extracted with EtOAc (2×30 mL). The combined organicphase was washed with brine (2×20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated to give 282.5 (750 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.96-3.79 (m, 1H), 3.76-3.70 (m, 1H),3.65-3.59 (m, 1H), 3.51-3.41 (m, 1H), 3.40-3.34 (m, 5H), 3.24-3.15 (m,1H), 2.50 (br d, J=14.0 Hz, 1H), 2.31-2.12 (m, 2H), 1.96-1.76 (m, 3H),1.73-1.55 (m, 7H), 1.51-1.48 (m, 1H), 1.47-1.32 (m, 5H), 1.30-1.29 (m,1H), 1.23-1.13 (m, 4H), 1.11-0.99 (m, 5H), 0.98-0.84 (m, 5H), 0.80-0.71(m, 1H), 0.68 (d, J=3.6 Hz, 3H).

Synthesis of 282 & 283

To a solution of 282.5 (750 mg, 1.90 mmol) in DMF (10 mL) were addedPh₃P (996 mg, 3.80 mmol), DEAD (212 mg, 2.28 mmol) and1H-pyrazole-4-carbonitrile (598 mg, 2.28 mmol) 0° C. After stirring at20° C. for 16 h, the mixture was poured into water (10 mL) and extractedwith EtOAc (2×20 mL). The combined organic phase was washed with brine(10 mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give282.6 (400 mg) as oil, which was purified by pre-HPLC (Welch Xtimate C18150×25 mm, 5 □m; Condition: water (0.225% FA)-ACN; Gradient: from 52% to82% of B in 8.5 min and hold 100% for 2 min; Flow rate: 30 mL/min) toafford 283 (60 mg) and 282 (20 mg) both as solids.

282 (20 mg) was further purified by SFC (Column: DAICEL CHIRALPAK AD-H250 mm×30 mm, 5 □m; Condition: 0.1% NH₃H₂O ETOH; Gradient: from 45% to45% of B; Flow rate: 50 mL/min; Column temperature: 35° C.) to afford282 (6.6 mg, 33%) as a solid.

283 (60 mg) was further purified by SFC (Column: DAICEL CHIRALPAK AD-H250 mm×30 mm, 5□m; Condition: 0.1% NH₃H₂O ETOH; Gradient: from 40% to40% of B; Flow rate: 50 mL/min; Column temperature: 35° C.) to afford283 (8.4 mg, 14%) as a solid.

282: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.24 (dd,J=3.6, 13.6 Hz, 1H), 3.84 (br s, 1H), 3.74-3.68 (m, 1H), 3.41-3.39 (m,5H), 2.62 (s, 1H), 2.49 (br d, J=14.4 Hz, 1H), 2.25 (dd, J=4.8, 12 Hz,1H), 2.05-1.95 (m, 2H), 1.94-1.77 (m, 3H), 1.68-1.62 (m, 3H), 1.40 (brd, J=16 Hz, 5H), 1.26-1.16 (m, 6H), 1.06 (s, 3H), 1.01-0.95 (m, 1H),0.89-0.85 (m, 1H), 0.83 (d, J=6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₆H₃₇N₃ [M-2H₂O-MeOH]⁺402.3 found 402.3.SFC 99.32% de.

283: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.50 (dd,J=4.4, 13.2 Hz, 1H), 3.92-3.82 (m, 1H), 3.70-3.63 (m, 1H), 3.43-3.39 (m,5H), 2.63 (s, 1H), 2.51-2.44 (m, 1H), 2.19 (dd, J=4.9, 11.7 Hz, 1H),2.15-2.06 (m, 1H), 1.89 (br d, J=13.6 Hz, 3H), 1.64 (br s, 4H),1.44-1.32 (m, 5H), 1.29-1.17 (m, 5H), 1.07 (s, 4H), 1.05-0.96 (m, 1H),0.90-0.86 (m, 1H), 0.79 (s, 3H), 0.67 (d, J=6.4 Hz, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₆H₃₇N₃ [M-2H₂O-MeOH]⁺402.3 found 402.3SFC 100% de.

Examples 285-290: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(285)&1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-5-carbonitrile(286) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(287) &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-3-carbonitrile(288)

Synthesis of 285.1 & 287.1

To a solution of 265.3 (400 mg, 0.906 mmol) in DMF (10 mL) was added1H-pyrazole-3-carbonitrile (168 mg, 1.81 mmol) and Cs₂CO₃ (589 mg, 1.81mmol) at 25° C. After stirring at 60° C. for 4 h, the mixture was cooledto 25° C., poured into water (20 mL) and extracted with EtOAc (2×50 mL).The combined organic phase was washed with brine (20 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 10%˜ 20% of EtOAc in PE) to give 285.1 (50 mg, 12.1%)as a solid and 287.1 (400 mg, 97.3%) as an oil.

285.1: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.60-7.53 (m, 1H), 6.80-6.74 (m,1H), 4.64-4.56 (m, 0.5H), 4.41-4.34 (m, 0.5H), 3.96-3.85 (m, 1H),3.57-3.49 (m, 2H), 3.48-3.38 (m, 2H), 2.79-2.60 (m, 1H), 2.25-2.06 (m,1H), 2.00-1.73 (m, 4H), 1.70-1.57 (m, 4H), 1.51-1.34 (m, 7H), 1.30-1.17(m, 7H), 1.15-0.98 (m, 5H), 0.85-0.79 (m, 3H), 0.74-0.65 (m, 3H).

287.1: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.43-7.36 (m, 1H), 6.65 (s, 1H),4.55-4.47 (m, 0.6H), 4.31-4.24 (m, 0.4H), 3.79-3.64 (m, 1H), 3.58-3.49(m, 2H), 3.48-3.39 (m, 2H), 2.99-2.88 (m, 1H), 2.22-2.01 (m, 2H),1.97-1.70 (m, 9H), 1.67-1.57 (m, 3H), 1.50-1.34 (m, 4H), 1.27-1.18 (m,5H), 1.13-1.03 (m, 5H), 0.79 (s, 3H), 0.72-0.65 (m, 3H).

Separation of 285 & 286

285.1 (50 mg, 0.110 mmol) was purified by SFC (Column: YMC CHIRALAmylose-C 250×30 mm, 10 um; Condition: 0.1% NH₃H₂O ETOH; Gradient: from45% to 45% B; Flow rate: 80 mL/min; Column temperature: 35° C.) toafford 285 (Peak 1, Rt=1.315 min, 21.7 mg, 43.4%) and 286 (Peak 2,Rt=2.181 min, 15.4 mg, 30.8%) as solids. The two diastereomers wereassigned based on ¹H NMR of C21-Me (C21-down-Me is at more downfieldthan C21-up isomer).

285: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58-7.54 (m, 1H), 6.79-6.76 (m, 1H),4.64-4.55 (m, 1H), 3.95-3.86 (m, 1H), 3.57-3.49 (m, 2H), 3.47-3.38 (m,2H), 2.70 (s, 1H), 2.26-2.12 (m, 1H), 1.93-1.70 (m, 5H), 1.69-1.57 (m,4H), 1.50-1.32 (m, 7H), 1.29-1.18 (m, 6H), 1.15-1.02 (m, 5H), 0.82 (s,3H), 0.68 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₈H₄₂N₃O [M−H₂O+H]⁺436.3 found 436.3. SFC 100% de.

286: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.57 (s, 1H), 6.77 (s, 1H), 4.41-4.34(m, 1H), 3.96-3.87 (m, 1H), 3.56-3.49 (m, 2H), 3.47-3.38 (m, 2H), 2.69(s, 1H), 2.17-2.05 (m, 1H), 2.00-1.90 (m, 2H), 1.84-1.75 (m, 3H),1.66-1.61 (m, 3H), 1.50-1.35 (m, 8H), 1.25-1.18 (m, 6H), 1.14-1.01 (m,5H), 0.81 (d, J=6.4 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₂N₃O [M−H₂O+H]⁺436.3 found 436.3. SFC 99% de.

Separation of 287 & 288

287.1 (400 mg, 0.909 mmol) was purified by SFC (Column: DAICEL CHIRALCELOD-H 250×30 mm, Sum; Condition: 0.1% NH₃H₂O ETOH; Gradient: from 30% to30% B; Flow rate: 60 mL/min; Column temperature: 35° C.) to afford 288(Peak 1, Rt=1.449 min, 67 mg, 16.2%) and 287 (Peak 2, Rt=1.639 min,106.6 mg, 25.7%) as solids. The two diastereomers were assigned based on¹H NMR of C21-Me (C21-down-Me is at more downfield than C21-up isomer).

287: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.57 (s, 1H), 6.77 (s, 1H), 4.41-4.34(m, 1H), 3.96-3.87 (m, 1H), 3.56-3.49 (m, 2H), 3.47-3.38 (m, 2H), 2.69(s, 1H), 2.17-2.05 (m, 1H), 2.00-1.90 (m, 2H), 1.84-1.75 (m, 3H),1.66-1.61 (m, 3H), 1.50-1.35 (m, 8H), 1.25-1.18 (m, 6H), 1.14-1.01 (m,5H), 0.81 (d, J=6.4 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₂N₃O [M−H₂O+H]⁺436.3 found 436.3. SFC 100% de.

288: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58-7.54 (m, 1H), 6.79-6.76 (m, 1H),4.64-4.55 (m, 1H), 3.95-3.86 (m, 1H), 3.57-3.49 (m, 2H), 3.47-3.38 (m,2H), 2.70 (s, 1H), 2.26-2.12 (m, 1H), 1.93-1.70 (m, 5H), 1.69-1.57 (m,4H), 1.50-1.32 (m, 7H), 1.29-1.18 (m, 6H), 1.15-1.02 (m, 5H), 0.82 (s,3H), 0.68 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₈H₄₂N₃O [M−H₂O+H]⁺436.3 found 436.3. analytic SFC 100% de.

Example 289 & 290: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-13-methyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(289) &(3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(290)

To a solution of 265.3 (250 mg, 0.5662 mmol) and Cs₂CO₃ (368 mg, 1.13mmol) in DMF (10 mL) was added 5-methyl-2H-1,2,3,4-tetrazole (71.4 mg,0.8493 mmol) at 15° C. under N₂. After stirring at 80° C. for 16 h, theresulting solution was cooled to 15° C., poured into water (30 mL) andextracted with EtOAc (3×20 mL). The combined organic phase was washedwith brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 15% of EtOAcin PE) to give mixture of diastereomers (160 mg, 63%), which wereseparated by SFC (Condition: 0.1% NH₃H₂O ETOH; Begin B: 40%; End B: 40%;FlowRate(ml/min): 80) to give 290 (Peak 2, 30 mg, 18%, Rt=4.166 min) and289 (Peak 1, 16.6 mg, 10%, Rt=2.713 min) as solids.

289: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.53 (dd, J=3.6, 13.2 Hz, 1H), 4.28(dd, J=9.2, 13.2 Hz, 1H), 3.53 (q, J=6.8 Hz, 2H), 3.46-3.38 (m, 2H),2.69 (s, 1H), 2.54 (s, 3H), 2.14 (td, J=6.4, 10.0 Hz, 1H), 2.05-1.88 (m,2H), 1.86-1.52 (m, 7H), 1.51-1.29 (m, 6H), 1.28-1.00 (m, 12H), 0.85 (d,J=6.4 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS purity 97%, MS ESI calcd. forC₂₆H₄₃N₄O [M−H₂O+H]⁺427.3, found 427.3.

290: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.75 (dd, J=4.4, 13.2 Hz, 1H),4.80-4.70 (m, 1H), 4.24 (dd, J=10.8, 13.2 Hz, 1H), 3.53 (q, J=6.8 Hz,2H), 3.43 (q, J=9.2 Hz, 2H), 2.54 (s, 3H), 2.30-2.15 (m, 1H), 1.95-1.72(m, 5H), 1.71-1.55 (m, 5H), 1.51-1.17 (m, 12H), 1.16-0.98 (m, 5H), 0.81(s, 3H), 0.71 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₆H₄₃N₄O [M−H₂O+H]⁺427.3, found 427.3.

Examples 293 & 294: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17R)-13-methyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(293) &(3R,5R,8R,9R,10S,13S,14S,17R)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(294)

Synthesis of 293.1

To a solution of K7 (185 mg, 0.43 mmol) in DMF (10 mL) were added Cs₂CO₃(283 mg, 0.87 mmol) and 5-methyl-2H-1,2,3,4-tetrazole (73.1 mg, 0.87mmol). After stirring at 80° C. for 16 h, the mixture was added intosaturated NH₄Cl (100 mL) and extracted with EtOAc (3×30 mL). Thecombined organic layer was washed with water (2×100 mL), brine (100 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜50% of EtOAc in PE) to give 293.1 (100 mg,54%) and 293.1a (60 mg, 32%) both as oils.

293.1: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.80-4.70 (m, 1H), 4.56-4.44 (m,1H), 4.35-4.15 (m, 1H), 2.53 (s, 3H), 2.30-2.10 (m, 1H), 1.99-1.59 (m,9H), 1.52-1.22 (m, 12H), 1.20-0.90 (m, 10H), 0.88-0.80 (m, 3H),0.75-0.66 (m, 3H).

293.1a: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.60-4.50 (m, 1H), 4.35-4.25 (m,1H), 3.90-3.75 (m, 1H), 2.55 (d, J=2.4 Hz, 3H), 2.08-1.59 (m, 9H),1.52-1.22 (m, 13H), 1.20-1.02 (m, 7H), 0.98-0.90 (m, 3H), 0.84-0.78 (m,3H), 0.76-0.65 (m, 3H).

Synthesis of 293 & 294

The diastereomeric mixture 293.1 (100 mg, 0.23 mmol) was separated bySFC (Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, 5□m), Condition: 0.1%NH₃H₂O ETOH, Begin B: 45%, End B: 45%, FlowRate(ml/min): 60) to give 293(24 mg, 24%, Rt=4.068) and 294 (39 mg, 39%, Rt=5.548) both as solids.

293: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.52 (dd, J=4.0 Hz, 13.2 Hz, 1H),4.35-4.24 (m, 1H), 2.54 (s, 3H), 2.20-1.90 (m, 3H), 1.89-1.59 (m, 6H),1.52-1.26 (m, 11H), 1.25-1.02 (m, 10H), 0.98-0.92 (m, 3H), 0.91-0.80 (m,3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₆H₄₃N₄[M+H—H₂O]⁺411.3, found 411.3, SFC: 100% de.

294: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.75 (dd, J=4.4 Hz, 13.2 Hz, 1H),4.30-4.18 (m, 1H), 2.53 (s, 3H), 2.30-2.18 (m, 1H), 1.95-1.59 (m, 8H),1.52-1.28 (m, 13H), 1.26-1.02 (m, 8H), 0.98-0.90 (m, 3H), 0.81 (s, 3H),0.76-0.66 (m, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₆H₄₃N₄[M+H—H₂O]⁺411.3, found 411.3, SFC: 100% de.

Examples 295-296: Synthesis of(3R,5S,8R,9R,10S,13R,14S,17R)-3-(methoxymethyl)-13-methyl-17-(2-(5-methyl-2H-tetrazol-2-yl)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(295) &(3R,5S,8R,9R,10S,13R,14S,17R)-3-(methoxymethyl)-13-methyl-17-(2-(5-methyl-1H-tetrazol-1-yl)ethyl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(296)

Synthesis of 295.1

To a stirred solution of NaH (2.05 g, 51.4 mmol, 60% in mineral oil) inTHF (100 mL) and was added ethyl 2-(diethoxyphosphanyl)acetate (12.2 g,54.5 mmol) at 25° C. After stirring at 40° C. for 30 mins under N₂,295.0 (5 g, 15.6 mmol) was added. After stirring at 65° C. for 16 h, theresulting mixture was cooled to 25° C. and poured into NH₄Cl (100 mL,sat.). The aqueous layer was extracted with EtOAc (3×60 mL). Thecombined organic phase was washed with brine (2×100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 10% of EtOAc in PE) to give 295.1 (5 g, 82%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.52 (s, 1H), 4.20-4.05 (m, 2H), 3.39 (s,3H), 3.19 (s, 2H), 2.85-2.75 (m, 2H), 2.01 (s, 1H), 1.95-1.59 (m, 8H),1.52-1.25 (m, 9H), 1.24-0.95 (m, 5H), 0.82 (s, 3H), 0.80-0.65 (m, 2H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₄H₃₉O₄ [M+H]⁺ 391.3, found391.3.

Synthesis of 295.2

A mixture of 295.1 (4.8 g, 12.2 mmol) and wet Pd/C (1 g, 10%) in EtOH(100 mL) was stirred at 25° C. for 16 h under H₂. The mixture wasfiltered, and the mother liquor was concentrated to give 295.2 (4 g,84%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.20-4.05 (m, 2H), 3.38 (s, 3H), 3.18 (s,2H), 2.40-2.30 (m, 1H), 2.15-1.60 (m, 9H), 1.52-1.25 (m, 9H), 1.20-0.90(m, 9H), 0.80-0.63 (m, 2H), 0.60 (s, 3H).

Synthesis of 295.3

To a solution of 295.2 (3.9 g, 9.9 mmol) in THF (100 mL) was addedLiAlH₄ (376 mg, 9.9 mmol) at 25° C. After stirring at 25° C. for 30mins, HCl (50 mL, 1 M) was added to the mixture. The aqueous layer wasextracted with EtOAc (3×50 mL). The combined organic layer was washedwith NaHCO₃ (100 mL, sat.), brine (100 mL), dried over anhydrous Na₂SO₄,filtered and concentrated to give 295.3 (3.4 g, 98%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.58 (m, 2H), 3.38 (s, 3H), 3.18 (s,2H), 2.00 (s, 1H), 1.95-1.59 (m, 9H), 1.50-1.20 (m, 7H), 1.19-0.95 (m,9H), 0.75-0.65 (m, 2H), 0.59 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₂H₃₇O₂ [M−H₂O+H]⁺333.3, found 333.3.

Synthesis of 295.4

To a solution of 295.3 (800 mg, 2.3 mmol) in DCM (20 mL) were added PPh₃(1.19 g, 4.6 mmol) and NBS (811 mg, 4.6 mmol) at 0° C. After stirring at25° C. for 16 h, the mixture was poured into water (100 mL) andextracted with DCM (2×50 mL). The combined organic layer was washed withbrine (100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated.The residue was purified by flash column (0˜5% of EtOAc in PE) to give295.4 (800 mg, 85%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.49-3.22 (m, 5H), 3.18 (s, 2H), 1.99-1.59(m, 10H), 1.50-1.20 (m, 7H), 1.19-0.65 (m, 10H), 0.60 (s, 3H).

Synthesis of 295 & 296

To a solution of 295.4 (300 mg, 0.73 mmol) in DMF (10 mL) were addedCs₂CO₃ (472 mg, 1.5 mmol) and 5-methyl-1,2,3,4-tetrazole (121 mg, 1.5mmol). After stirring at 80° C. for 16 h, the mixture was poured intowater (100 mL) and extracted with EtOAc (3×50 mL). The combined organiclayer was washed with LiCl (2×100 mL, 4% in water), brine (100 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜ 40% of EtOAc in PE) to give 295 (94 mg,31%) and 296 (77 mg, 25%) both as solids.

295: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.60-4.40 (m, 2H), 3.38 (s, 3H), 3.18(s, 2H), 2.53 (s, 3H), 2.20-1.95 (m, 2H), 1.95-1.59 (m, 9H), 1.52-0.90(m, 14H), 0.78-0.64 (m, 2H), 0.61 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₄H₄₁N₄O₂ [M+H]⁺ 417.3, found 417.3.

296: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.22 (t, J=8.0 Hz, 2H), 3.38 (s, 3H),3.18 (s, 2H), 2.55 (s, 3H), 2.14-1.97 (m, 2H), 1.95-1.59 (m, 9H),1.52-0.90 (m, 14H), 0.78-0.64 (m, 2H), 0.61 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₄H₄₁N₄O₂ [M+H]⁺ 417.3, found 417.3.

Examples 297&298: Synthesis of(3R,5S,8R,9R,10S,13R,14S,17R)-17-(2-(2H-1,2,3-triazol-2-yl)ethyl)-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(297) &(3R,5S,8R,9R,10S,13R,14S,17R)-17-(2-(1H-1,2,3-triazol-1-yl)ethyl)-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(298)

To a solution of 295.4 (300 mg, 0.73 mmol) in DMF (10 mL) were addedCs₂CO₃ (472 mg, 1.5 mmol) and 2H-1,2,3-triazole (100 mg, 1.5 mmol).After stirring at 80° C. for 16 h, the mixture was added into water (100mL) and extracted with EtOAc (3×50 mL). The combined organic layer waswashed with LiCl (2×100 mL, 4% in water), brine (100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 40% of EtOAc in PE) to give 297 (50 mg, 17%) and 298(65 mg, 22%) both as solids.

297: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.58 (s, 2H), 4.50-4.31 (m, 2H), 3.38(s, 3H), 3.18 (s, 2H), 2.15-2.05 (m, 1H), 1.98 (s, 1H), 1.90-1.59 (m,9H), 1.50-0.85 (m, 14H), 0.75-0.64 (m, 2H), 0.61 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₄H₄₀N₃O₂ [M+H]⁺ 402.3, found 402.3.

298: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.70 (s, 1H), 7.53 (s, 1H), 4.48-4.25(m, 2H), 2.10-1.98 (m, 2H), 1.95-1.59 (m, 9H), 1.50-0.90 (m, 14H),0.75-0.64 (m, 2H), 0.61 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₄H₄₀N₃O₂ [M+H]⁺ 402.3, found 402.3.

Examples 299 & 300: Synthesis of1-((R)-2-((3S,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-isobutyl-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(299) &1-((S)-2-((3S,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-isobutyl-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(300)

Synthesis of 299.1

To a solution of 2,6-di-tert-butyl-4-methylphenol (48.2 g, 218 mmol) intoluene (300 mL) was added dropwise AlMe₃ (54.4 mL, 109 mmol, 2 M intoluene) at 0° C. After stirring at 30° C. for 30 min, the MAD solutionwas cooled to −70° C. and a solution of 261.1 (10 g, 36.4 mmol) in DCM(20 mL) was added dropwise at −70° C. After stirring at −70° C. for 1 hunder N₂, isobutylmagnesium chloride (54.5 mL, 109 mmol, 2 M in THF) wasadded dropwise at −70° C. After stirring at −70° C. for 4 h, thereaction mixture was poured into saturated aqueous citric acid (300 mL)at 10° C. and extracted with EtOAc (2×200 mL). The combined organiclayer was dried over Na₂SO₄, filtered and concentrated in vacuum. Theproduct was purified by flash column (0˜30% of EtOAc in PE) to giveproduct 299.1 (8.2 g, 67%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.44 (dd, J=8.0, 18.8 Hz, 1H), 2.14-2.01(m, 1H), 1.98-1.89 (m, 1H), 1.86-1.60 (m, 8H), 1.56-1.34 (m, 10H),1.20-1.07 (m, 3H), 0.97 (d, J=6.8 Hz, 6H), 0.90-0.82 (m, 6H).

Synthesis of 299.2

To a suspension of Ph₃PEtBr (17.8 g, 48.0 mmol) in anhydrous THF (150mL) was added t-BuOK (5.37 g, 48.0 mmol) at 25° C. under N₂. Afterstirring at 50° C. for 30 mins, a solution of 299.1 (8 g, 24.0 mmol) inanhydrous THF (50 mL) was added dropwise. After stirring at 50° C. for16 h, the mixture was poured into saturated NH₄Cl (500 mL), stirred for10 mins. and extracted with EtOAc (2×200 mL). The combined organic phasewas washed with brine (2×200 mL), filtered and concentrated. The residuewas purified by flash column (0˜30% of EtOAc in PE) to give 299.2 (6 g,72.6%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.19-5.04 (m, 1H), 2.40-2.14 (m, 3H),1.88-1.59 (m, 10H), 1.52-1.37 (m, 7H), 1.35-1.02 (m, 11H), 0.97 (dd,J=1.6, 6.8 Hz, 6H), 0.87 (s, 3H).

Synthesis of 299.3

To a solution of 299.2 (6.0 g, 17.4 mmol) in THF (70 mL) was added 9-BBNdimer (4.24 g, 34.8 mmol) at 20° C. After stirring at 50° C. for 16 h,the reaction was cooled to 0° C. and sequentially treated with ethanol(19.8 ml, 347 mmol), NaOH (69.4 mL, 5 M, 347 mmol) slowly, and finallyH₂O₂ (34.7 mL, 347 mmol, 30%) slowly below 15° C. After stirring at 75°C. for 1 h, the reaction mixture was poured into saturated aqueousNa₂S₂O₃ (500 mL) at 0° C. and stirred at 0° C. for 1 h. The reaction waschecked by potassium iodide-starch test paper to confirm excess H₂O₂ wasdestroyed. The mixture was extracted with EtOAc (2×200 mL). The combinedorganic layer was washed with brine (2×500 mL), drive over anhydrousNa₂SO₄, filtered and concentrated in vacuum to give the product 299.3(7.2 g) as a solid.

Synthesis of 299.4

To a solution of 299.3 (7.0 g, 19.3 mmol) in DCM (300 mL) and was addedPCC (12.4 g, 57.9 mmol) and silica gel (14 g). After stirring at 25° C.for 2 h, the precipitate was filtered and the filtrate was concentratedunder vacuum. The residue was purified by flash column (0˜30% of EtOAcin PE) to give product 299.4 (3.5 g, 50.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.54 (t, J=8.8 Hz, 1H), 2.11 (s, 3H),2.04-1.96 (m, 1H), 1.87-1.59 (m, 8H), 1.55-1.32 (m, 11H), 1.30-1.02 (m,7H), 0.97 (dd, J=1.2, 6.4 Hz, 6H), 0.61 (s, 3H).

Synthesis of 299.5

To a mixture of MePPh₃Br (5.9 g, 16.6 mmol) in THF (50 mL) was addedt-BuOK (1.9 g, 16.6 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 mins, 299.4 (2 g, 5.5 mmol) was added in portions below 50° C.After stirring at 50° C. for 1 h, the reaction mixture was quenched with10% NH₄Cl aqueous (200 mL) at 15° C. and extracted with EtOAc (300 mL).The combined organic phase was concentrated under vacuum to give asolid, which was purified by silica gel chromatography (PE/EtOAc=20/1 to5/1) to afford 299.5 (1.7 g, 85.8%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.07-2.00 (m,1H), 1.86-1.65 (m, 12H), 1.52-1.32 (m, 10H), 1.29-1.06 (m, 8H), 0.97(dd, J=0.8, 6.8 Hz, 6H), 0.57 (s, 3H).

Synthesis of 299.6

To a solution of 299.5 (1 g, 2.78 mmol) in THF (20 mL) was added boranedimethylsulfide (0.84 mL, 10 M 8.34 mmol). After stirring at 45° C. for1 h, the reaction mixture was sequentially diluted with ethanol (1.91 g,41.7 mmol) at 15° C., followed by NaOH aqueous (8.3 mL, 5.0 M, 41.7mmol) at 15° C. and finally H₂O₂ (4.2 mL, 10 M, 41.7 mmol) dropwise at15° C. After stirring at 78° C. for 1 h, the reaction mixture wasquenched with saturated aqueous Na₂S₂O₃ (500 mL) at 0° C. and stirred at0° C. for 1 h. The reaction was checked by potassium iodide-starch testpaper to confirm excess H₂O₂ was destroyed. The mixture was cooled andadded to water (1000 mL). The mixture was filtered. The filter cake waswashed with water (3×500 mL), dried under vacuum to give 299.6 (1 g) asa solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.60 (m, 1H), 3.49-3.32 (m, 1H),2.00-1.53 (m, 12H), 1.50-1.27 (m, 12H), 1.25-0.98 (m, 12H), 0.95-0.77(m, 3H), 0.68 (s, 3H).

Synthesis of 299.7

To a solution of 299.6 (500 mg, 1.32 mmol) in DCM (5 mL) at 0° C. wasadded PPh₃ (519 mg, 1.98 mmol) and NBS (352 mg, 1.98 mmol). Afterstirring at 20° C. for 2 h to give a solution, the reaction mixture wasadded water (50 mL) and extracted with DCM (2×50 mL). The combinedorganic phase was washed with brine (100 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜15% of EtOAc in PE) to give 299.7 (400 mg, 68.9%) as a oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.66-3.47 (m, 1H), 3.42-3.29 (m, 1H),1.96-1.72 (m, 6H), 1.69-1.58 (m, 3H), 1.52-1.39 (m, 5H), 1.37-1.16 (m,10H), 1.15-0.99 (m, 8H), 0.97 (dd, J=1.6, 6.8 Hz, 6H), 0.70-0.67 (m,3H).

Synthesis of 299.8

To a solution of 299.7 (400 mg, 910 μmol) and 1H-pyrazole-4-carbonitrile(101 mg, 1.09 mmol) in DMF (10 mL) was added Cs₂CO₃ (592 mg, 1.82 mmol)at 20° C. under N₂. After stirring at 80° C. for 16 h, the reactionmixture was quenched with saturated aq. NH₄Cl solution (50 mL) andextracted with EtOAc (2×30 mL). The combined organic phase was washedwith brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give the product 299.8 (260 mg, 63.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.49 (dd,J=4.4, 13.2 Hz, 1H), 4.25 (dd, J=4.0, 14.0 Hz, 1H), 3.78-3.59 (m, 2H),2.17-2.01 (m, 1H), 1.96-1.60 (m, 10H), 1.49-1.27 (m, 11H), 1.22-1.08 (m,5H), 1.00-0.95 (m, 6H), 0.83-0.78 (m, 3H), 0.73-0.66 (m, 3H).

Synthesis of 299 & 300

299.8 was separated by SFC Column: DAICEL CHIRALCEL OJ-H (250 mm*30 mm,5 um); condition: 0.1% NH₃H₂O EtOH; Begin B: 25%; End B: 25%;) to afford299 (121.7 mg, 48.5%, Rt=3.217 min) as a solid and 300 (78.2 mg, 31.4%,Rt=2.963 min) as a solid.

299: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.56-4.42(m, 1H), 3.71-3.59 (m, 1H), 2.17-2.07 (m, 1H), 1.91-1.58 (m, 10H),1.52-1.31 (m, 12H), 1.26-1.12 (m, 6H), 0.99-0.95 (m, 6H), 0.79 (s, 3H),0.68 (d, J=6.4 Hz, 3H). LC-ELSD/MS: purity 99%, analytic SFC: 98.1% de;MS ESI calcd. for C₂₉H₄₄N₃ [M−H₂O+H]⁺434.3, found 434.3. SFC 100% de.

300: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.26 (dd,J=4.0, 13.6 Hz, 1H), 3.77-3.67 (m, 1H), 2.05-1.90 (m, 3H), 1.83-1.60 (m,8H), 1.49-1.33 (m, 9H), 1.29-1.06 (m, 9H), 0.98-0.95 (m, 6H), 0.81 (d,J=6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS: purity 99%, analytic SFC: 100%de; MS ESI calcd. for C₂₉H₄₄N₃ [M−H₂O+H]⁺434.3, found 434.3. SFC 100%de.

Examples 301 & 302: Synthesis of1-((R)-2-((3R,5R,8S,9S,10S,13S,14S,17R)-10-ethyl-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(301) &1-((S)-2-((3R,5R,8S,9S,10S,13S,14S,17R)-10-ethyl-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(302)

Synthesis of 301.1

To a solution of 301.0 (90 g, 229 mmol) in DCM (500 mL) was added silicagel (80 g) and PCC (73.7 g, 343 mmol) in portions at 15° C. Afterstirring at 15° C. for 0.5 h, the mixture was filtered and the filtercake was washed with DCM (100 mL). The combined filtrate wasconcentrated to give 301.1 (87 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 9.56 (s, 1H), 4.01-3.75 (m, 8H), 2.24-2.12(m, 1H), 2.02-1.86 (m, 3H), 1.83-1.70 (m, 3H), 1.67-1.37 (m, 12H),1.28-1.17 (m, 2H), 1.09-0.80 (m, 4H).

Synthesis of 301.2

To a suspension of MePPh₃Br (145 g, 408 mmol) in THF (300 mL) was addedt-BuOK (45.7 g, 408 mmol) at 15° C. After stirring at 45° C. for 0.5 h,a solution of 301.1 (80 g, 204 mmol) in THF (200 mL) was added at 45° C.After stirring at 45° C. for 1 h, the mixture was diluted with PE (300mL) and filtered. The filtrate was concentrated to give 301.2 (200 g) asoil. 301.2 (600 g) was treated with PE (1 L) and stirred for 16 h. Thesuspension was filtered and the filtrate was concentrated to give 301.2(252 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 6.30 (dd, J=11.2, 17.6 Hz, 1H), 5.15-4.96(m, 2H), 3.94-3.81 (m, 8H), 2.02-1.73 (m, 7H), 1.58-1.35 (m, 13H),1.22-1.14 (m, 2H), 0.81 (s, 3H).

Synthesis of 301.3

To a solution of 301.2 (100 g, 257 mmol) in THF (1 L) was added 12 M HCl(107 mL, 1285 mmol). After stirring at 15° C. for 16 h, the reactionmixture was diluted with H₂O (800 mL), treated with solid Na₂CO₃ (200 g)until pH=9, and extracted with EtOAc (3×500 mL). The combined organiclayers were dried over Na₂SO₄, filtered and concentrated to give theproduct (100 g). The product (200 g) was purified by flash column (0˜30%of EtOAc in PE) to give the product 301.3 (80 g, 40.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 6.31 (dd, J=11.2, 17.6 Hz, 1H), 5.19 (d,J=11.2 Hz, 1H), 5.09 (d, J=17.6 Hz, 1H), 2.71 (t, J=15.2 Hz, 1H), 2.46(dd, J=8.8, 19.2 Hz, 1H), 2.37-2.21 (m, 2H), 2.17-2.06 (m, 4H),2.00-1.83 (m, 3H), 1.71-1.51 (m, 7H), 1.40-1.26 (m, 4H), 0.87 (s, 3H).

Synthesis of 301.4

To a mixture of 301.3 (80 g, 266 mmol) in THF (1 L) was added Pd—C(wet,50%, 10 g) under N₂. The suspension was degassed under vacuum and purgedwith H₂ for three times. After hydrogenating at 30 psi of hydrogen at25° C. for 16 h, the reaction mixture was filtered through a pad ofCelite and washed with THF (3×200 mL). The filtrate was concentrated togive the product 301.4 (80 g, 99.5%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.68 (t, J=13.6 Hz, 1H), 2.46 (dd, J=8.8,19.2 Hz, 1H), 2.38-2.27 (m, 1H), 2.24-2.16 (m, 1H), 2.13-2.06 (m, 2H),2.01-1.92 (m, 1H), 1.88-1.69 (m, 6H), 1.65-1.51 (m, 4H), 1.44-1.16 (m,7H), 0.88 (s, 3H), 0.81 (t, J=7.6 Hz, 3H).

Synthesis of 301.5

To a solution of 2,6-di-tert-butyl-4-methylphenol (40.1 g, 182 mmol) intoluene (100 mL) was added dropwise AlMe₃ (45.6 mL, 91.2 mmol, 2M intoluene) at 0° C. The mixture was stirred at 20° C. for 30 mins to givea MAD solution. To the MAD (91.2 mmol in 145 mL toluene) solution wasadded a solution of 301.4 (9.2 g, 30.4 mmol) in DCM (50 mL) dropwise at−70° C. After stirring at −70° C. for 1 h under N₂, n-PrMgCl (45.6 mL,91.2 mmol, 2 M in THF) was added dropwise at −70° C. The resultingsolution was stirred at −70° C. for another 2 h. The reaction mixturewas poured into saturated aqueous citric acid (300 mL) below 10° C. andextracted with EtOAc (2×300 mL). The combined organic layer was driedover Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜25% of EtOAc in PE) to give 301.5 (6.2 g, 59%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.51-2.37 (m, 1H), 2.15-2.00 (m, 1H),1.97-1.61 (m, 8H), 1.54-1.45 (m, 6H), 1.41-1.18 (m, 13H), 0.94 (t, J=7.2Hz, 3H), 0.85 (s, 3H), 0.81 (t, J=7.6 Hz, 3H).

Synthesis of 301.6

To a suspension of Ph₃PEtBr (19.2 g, 51.9 mmol) in anhydrous THF (100mL) was added t-BuOK (5.81 g, 51.9 mmol) at 25° C. under N₂. Afterstirring at 50° C. for 30 mins, a solution of 301.5 (6 g, 17.3 mmol) inanhydrous THF (50 mL) was added dropwise. After stirring at 50° C. for16 h, the mixture was poured into saturated NH₄Cl (300 mL), stirred for10 mins. and extracted with EtOAc (2×200 mL). The combined organic phasewas washed with brine (2×200 mL), filtered and concentrated. The residuewas purified by flash column (0˜20% of EtOAc in PE) to give the product301.6 (5.6 g, 90%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.18-4.95 (m, 1H), 2.41-2.11 (m, 3H), 1.88(t, J=12.8 Hz, 1H), 1.81-1.71 (m, 1H), 1.67-1.59 (m, 6H), 1.54-1.39 (m,9H), 1.36-1.25 (m, 7H), 1.20-1.13 (m, 3H), 0.94 (t, J=7.2 Hz, 3H),0.89-0.83 (m, 5H), 0.79 (t, J=7.6 Hz, 3H).

Synthesis of 301.7

To a solution of 301.6 (5.5 g, 15.3 mmol) in THF (80 mL) under N₂ wasadded 9-BBN dimer (7.46 g, 30.6 mmol). After stirring at 20° C. for 16h, ethanol (14.0 g, 306 mmol) was added, followed by NaOH aqueous (45.8mL, 5.OM, 229 mmol) at 0° C. Hydrogen peroxide (25.9 g, 229 mmol, 30% inH₂O) was then added dropwise at 0° C. After stirring at 70° C. for 1 h,the mixture was cooled to 15° C., diluted with water (200 mL) andsaturated aqueous Na₂SO₃ (200 mL). The aqueous phase was extracted withEtOAc (2×200 mL). The combined organic phase was washed with brine (200mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give theproduct. The product was triturated from MeCN (30 mL) to give 301.7 (6.5g) as a solid.

Synthesis of 301.8

To a mixture of 301.7 (6 g, 15.9 mmol) and silica gel (10 g) in DCM (150mL) was added PCC (8.53 g, 39.7 mmol) in portions. After stirring at 20°C. for 1 h, the reaction mixture was filtered and eluted with DCM (2×50mL). The filtrate was concentrated. The residue was purified by flashcolumn (0˜20% of EtOAc in PE) to give 301.8 (4.2 g, 70%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.53 (t, J=8.8 Hz, 1H), 2.22-2.09 (m, 4H),2.05-1.97 (m, 1H), 1.93-1.84 (m, 1H), 1.78-1.59 (m, 6H), 1.53-1.10 (m,20H), 0.95 (t, J=7.2 Hz, 3H), 0.79 (t, J=7.6 Hz, 3H), 0.59 (s, 3H).

Synthesis of 301.9

To a suspension of MePPh₃Br (1.14 g, 3.20 mmol) in anhydrous THF (10 mL)was added t-BuOK (537 mg, 4.80 mmol) at 25° C. under N₂. After stirringat 50° C. for 30 mins, a solution of 301.8 (600 mg, 1.60 mmol) inanhydrous THF (5 mL) was added dropwise. After stirring at 50° C. for 16h, the mixture was poured into saturated NH₄Cl (30 mL), stirred for 10mins. and extracted with EtOAc (2×20 mL). The combined organic phase waswashed with brine (2×30 mL), filtered and concentrated. The residue waspurified by flash column (0˜20% of EtOAc in PE) to 301.9 (580 mg, 97%)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H), 2.03-1.80 (m,3H), 1.76-1.60 (m, 9H), 1.52-1.34 (m, 10H), 1.29-1.13 (m, 11H), 0.94 (t,J=7.2 Hz, 3H), 0.78 (t, J=7.6 Hz, 3H), 0.54 (s, 3H).

Synthesis of 301.10

To a solution of 301.9 (580 mg, 1.55 mmol) in THF (15 mL) was addedBH₃.Me₂S (465 μL, 4.65 mmol, 10M) at 20° under N₂. After stirring at 20°C. for 16 h, the resulting mixture was treated with ethanol (1.42 g,31.0 mmol) at 15° C. and NaOH aqueous (4.64 mL, 5.0 M, 23.2 mmol) at 0°C. Hydrogen peroxide (2.62 g, 23.2 mmol, 30% in H₂O) was then addeddropwise at 0° C. After stirring at 70° C. for 1 h, the mixture wascooled to 15° C., diluted with water (50 mL) and saturated aqueousNa₂SO₃ (50 mL). The reaction was then checked by potassium iodide-starchtest paper to confirm excess H₂O₂ was destroyed (did not changed toblue). The aqueous phase was extracted with EtOAc (2×50 mL). Thecombined organic phase was washed with brine (50 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 301.10 (600 mg) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.59 (m, 1H), 3.48-3.29 (m, 1H), 2.99(s, 1H), 1.97-1.72 (m, 4H), 1.64-1.58 (m, 3H), 1.52-1.25 (m, 17H),1.19-1.01 (m, 9H), 0.97-0.92 (m, 4H), 0.78 (t, J=7.6 Hz, 3H), 0.66 (s,3H)

Synthesis of 301.11

To a solution of 301.10 (600 mg, 1.53 mmol) in DCM (15 mL) at 0° C. wasadded PPh₃ (600 mg, 2.29 mmol) and NBS (407 mg, 2.29 mmol). Afterstirring at 20° C. for 2 h, the reaction mixture was diluted with water(20 mL) and extracted with DCM (2×20 mL). The combined organic phase waswashed with brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give 301.11 (500 mg, 72%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.66-3.46 (m, 1H), 3.40-3.26 (m, 1H),1.91-1.73 (m, 4H), 1.64-1.45 (m, 9H), 1.40-1.24 (m, 12H), 1.20-0.98 (m,9H), 0.94 (t, J=7.2 Hz, 3H), 0.78 (t, J=7.4 Hz, 3H), 0.71-0.61 (m, 3H).

Synthesis of 301.12

To a solution of 301.11 (500 mg, 1.10 mmol) and1H-pyrazole-4-carbonitrile (112 mg, 1.21 mmol) in DMF (10 mL) was addedCs₂CO₃ (721 mg, 2.20 mmol) at 20° C. under N₂. After stirring at 80° C.for 16 h, the reaction mixture was quenched with saturated aq. NH₄Clsolution (50 mL) and extracted with EtOAc (2×30 mL). The combinedorganic phase was washed with brine (50 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜30% of EtOAc in PE) to give the product 301.12 (250 mg, 49%)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.84-7.69 (m, 2H), 4.54-4.20 (m, 1H),3.79-3.57 (m, 1H), 2.03-1.58 (m, 8H), 1.53-1.09 (m, 23H), 0.94 (t, J=7.6Hz, 3H), 0.83-0.75 (m, 6H), 0.72-0.65 (m, 3H).

Separation of 301 & 302

301.12 (250 mg) was separated by SFC (Column:DAICEL CHIRALCEL OJ-H (250mm*30 mm, 5 um)); condition: 0.1% NH₃H₂O EtOH; Begin B: 25%; End B:25%;) to afford 301 (58.7 mg, 23%, Rt=3.254 min) and 302 (70.3 mg, 28%,Rt=2.949 min) as solids.

301: ¹H NMR (400 MHz, CDCl₃) δ 7.80 (s, 1H), 7.75 (s, 1H), 4.48 (dd,J=4.8, 13.6 Hz, 1H), 3.65 (dd, J=10.8, 13.6 Hz, 1H), 2.16-2.05 (m, 1H),1.94-1.58 (m, 8H), 1.53-1.31 (m, 12H), 1.30-1.03 (m, 10H), 0.94 (t,J=7.2 Hz, 3H), 0.82-0.75 (m, 6H), 0.67 (d, J=6.8 Hz, 3H). LC-ELSD/MSpurity 99%, analytic SFC: 97.72% de. MS ESI calcd. for C₃₀H₄₆N₃[M+H—H₂O]⁺448.4, found 448.4. SFC 100% de.

302: ¹H NMR (400 MHz, CDCl₃) δ 7.79 (s, 1H), 7.75 (s, 1H), 4.25 (dd,J=4.0, 13.6 Hz, 1H), 3.72 (dd, J=10.0, 13.6 Hz, 1H), 2.06-1.83 (m, 4H),1.80-1.70 (m, 1H), 1.69-1.57 (m, 4H), 1.53-1.32 (m, 12H), 1.30-1.11 (m,10H), 0.94 (t, J=7.2 Hz, 3H), 0.82-0.75 (m, 6H), 0.69 (s, 3H).LC-ELSD/MS purity 99%, analytic SFC: 98.04% de. MS ESI calcd. forC₃₀H₄₆N₃[M+H—H₂O]⁺448.4, found 448.4. SFC 100% de.

Examples 303 & 304: Synthesis of1-((R)-2-((3S,5R,8R,9R,10S,13S,14S,17R)-3-(cyclopropylmethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(303) &1-((S)-2-((3S,5R,8R,9R,10S,13S,14S,17R)-3-(cyclopropylmethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(304)

Synthesis of 303.1

To a MAD (72.8 mmol in 100 mL toluene) solution was added a solution ofA24 (10 g, 36.4 mmol) in DCM (30 mL) dropwise at −70° C. under N₂. Afterstirring at −70° C. for 1 h, bromo(prop-2-en-1-yl)magnesium (36.4 mL,36.4 mmol, 1M) was added dropwise. After stirring at −70° C. for 3 h,the reaction mixture was poured into 20% aqueous citric acid (200 mL) at10° C. and extracted with EtOAc (2×200 mL). The combined organic layerwas dried over Na₂SO₄, filtered and concentrated in vacuum. The residuewas purified by a silica gel column (PE/EtOAc=30-40%) to give 303.1 (8.8g, 77%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.96-5.80 (m, 1H), 5.25-5.08 (m, 2H),2.53-2.35 (m, 3H), 2.16-2.04 (m, 1H), 2.00-1.64 (m, 7H), 1.55-1.38 (m,5H), 1.36-1.24 (m, 5H), 1.22-1.02 (m, 5H), 0.87 (s, 3H).

Synthesis of 303.2

To a solution of Et₂Zn (37.8 mL, 1M in hexane) in DCM (120 mL) at 0° C.were added CF₃COOH (3.59 g, 31.5 mmol) dropwise over a period of 0.5 hunder N₂, followed by CH₂I₂ (10.1 g, 37.8 mmol) dropwise over a periodof 15 mins, finally a solution of 303.1 (2 g, 6.31 mmol) in DCM (30 mL).After stirring at 0° C. for 1 h and warming to 20° C. for 12 h, themixture was combined with another batch (from 2.0 g of 303.1) and addedinto saturated NH₄Cl (200 mL). The aqueous layer was extracted with DCM(3×50 mL). The combined organic layer was washed with brine (200 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜30% of EtOAc in PE) to give 303.2 (2.5 g) asa solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.44 (dd, J=8.4, 19.2 Hz, 1H), 2.15-1.90(m, 2H), 1.88-1.59 (m, 8H), 1.52-1.36 (m, 6H), 1.35-1.00 (m, 9H), 0.87(s, 3H), 0.80-0.70 (m, 1H), 0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₂H₃₃O [M+H—H₂O]⁺313.3, found313.3.

Synthesis of 303.3

To a solution of EtPh₃PBr (10.7 g, 29.0 mmol) in THF (25 mL) was addedt-BuOK (3.25, 29.0 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 minutes, a solution of 303.2 (2.4 g, 7.26 mmol) in THF (25 mL)was added to the reaction below 50° C. After stirring at 50° C. for 3 h,the mixture was added into saturated NH₄Cl (150 mL) and extracted withEtOAc (3×50 mL). The combined organic layer was washed with brine (100mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜10% of EtOAc in PE) to give 303.3 (2 g,81%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-5.05 (m, 1H), 2.45-2.08 (m, 3H),1.90-1.65 (m, 9H), 1.60-1.20 (m, 11H), 1.19-1.00 (m, 6H), 0.87 (s, 3H),0.85-0.74 (m, 1H), 0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H).

Synthesis of 303.4

To a solution of 303.3 (2 g, 5.83 mmol) in THF (30 mL) was added 9-BBNdimer (4.21 g, 17.4 mmol) at 45° C. After stirring for 16 h. thereaction mixture was sequentially treated with EtOH (20 mL) at 15° C.,NaOH (23.2 mL, 5M, 116 mmol) at 0° C. and finally by H₂O₂ (13.1 g, 30%,116 mmol). After stirring at 70° C. for 2 h, the reaction was dilutedwith water (150 mL) and then saturated Na₂S₂O₃ (100 mL). The mixture wasfiltered and the filter cake was washed with water (2×50 mL) andconcentrated to give 303.4 (2.05 g, 98%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.78-3.62 (m, 1H), 1.98-1.58 (m, 10H),1.52-1.25 (m, 11H), 1.23-1.00 (m, 10H), 0.82-0.70 (m, 1H), 0.66 (s, 3H),0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H).

Synthesis of 303.5

To a solution of 303.4 (2.1 g, 5.82 mmol) in DCM (40 mL) was added DMP(4.91 g, 11.6 mmol) at 30° C. After stirring for 1 h, the mixture wasadded into saturated NaHCO₃ (200 mL) and extracted with DCM (3×50 mL).The combined organic layer was washed with saturated Na₂S₂O₃ (2×200 mL),brine (200 mL), dried over anhydrous Na₂SO₄, filtered and concentratedto give 303.5 (2 g) as oil. 303.5 (1 g) was purified by flash column(0˜30% of EtOAc in PE) to give 303.5 (450 mg, 45%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.54 (t, J=8.8 Hz, 1H), 2.20-2.13 (m, 1H),2.12 (s, 3H), 2.05-1.96 (m, 1H), 1.90-1.60 (m, 9H), 1.55-1.30 (m, 9H),1.28-1.00 (m, 6H), 0.80-0.70 (m, 1H), 0.61 (s, 3H), 0.55-0.45 (m, 2H),0.15-0.05 (m, 2H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₄H₃₇₀[M+H—H₂O]⁺341.3, found 341.3.

Synthesis of 303.6

To a solution of MePh₃PBr (2.97 g, 8.34 mmol) in THF (20 mL) was addedt-BuOK (934 mg, 8.34 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 minutes, a solution of 303.5 (1 g, 2.78 mmol) was added to thereaction mixture below 50° C. After stirring at 50° C. for 1 h, themixture was added into saturated NH₄Cl (100 mL) and extracted with EtOAc(3×30 mL). The combined organic layer was washed with brine (100 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜5% of EtOAc in PE) to give 303.6 (600 mg,61%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.10-2.00 (m,1H), 1.89-1.59 (m, 13H), 1.52-1.26 (m, 9H), 1.25-0.95 (m, 6H), 0.93-0.70(m, 2H), 0.57 (s, 3H), 0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H).

Synthesis of 303.7

To a solution of 303.6 (600 mg, 1.68 mmol) in THF (10 mL) was addedBH₃Me₂S (0.5 mL, 10M in THF, 5.00 mmol) at 15° C. After stirring at 15°C. for 16 h, the reaction mixture was sequentially treated with EtOH (10mL) at 15° C., NaOH (6.72 mL, 5M in water, 33.6 mmol) at 0° C. andfinally H₂O₂ (3.80 g, 30%, 33.6 mmol) dropwise. After stirring at 70° C.for 2 h, the mixture was added into water (100 mL), stirred for 30minutes, and filtered. The filter cake was washed with water (2×50 mL),saturated Na₂S₂O₃ (100 mL) and concentrated to give 303.7 (550 mg, 87%)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.80-3.60 (m, 1H), 3.52-3.30 (m, 1H),2.00-1.59 (m, 11H), 1.52-1.10 (m, 13H), 1.08-0.75 (m, 9H), 0.69 (s, 3H),0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H).

Synthesis of 303.8

To a solution of 303.7 (500 mg, 1.33 mmol) in DCM (10 mL) was added NBS(473 mg, 2.66 mmol) and Ph₃P (697 mg, 2.66 mmol) at 0° C. under N₂.After stirring at 15° C. for 2 h, the mixture was concentrated andpurified by flash column (0˜10% of EtOAc in PE) to give 303.8 (450 mg)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.68-3.50 (m, 1H), 3.42-3.30 (m, 1H),2.00-1.59 (m, 9H), 1.52-1.20 (m, 13H), 1.18-0.95 (m, 6H), 0.93-0.70 (m,4H), 0.68 (s, 3H), 0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H).

Synthesis of 303.9

To a solution of 303.8 (450 mg) in DMF (10 mL) were added Cs₂CO₃ (535mg, 2.04 mmol) and 1H-pyrazole-4-carbonitrile (189 mg, 2.04 mmol) at 15°C. under N₂. After stirring at 80° C. for 2 h, the mixture was addedinto saturated NH₄Cl (100 mL) and extracted with EtOAc (3×30 mL). Thecombined organic layer was washed with water (2×200 mL), brine (200 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜30% of EtOAc in PE) to give 303.9 (240 mg,52%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.55-4.22 (m,1H), 3.75-3.60 (m, 1H), 2.18-2.07 (m, 1H), 1.99-1.59 (m, 10H), 1.52-1.28(m, 10H), 1.23-1.00 (m, 7H), 0.85-0.65 (m, 7H), 0.55-0.45 (m, 2H),0.15-0.05 (m, 2H).

Synthesis of 303 & 304

303.9 (240 mg, 0.53) was separated by SFC (Column: DAICEL CHIRALCEL OD(250 mm*30 mm, 10□m), Condition: 0.1% NH₃H₂O ETOH, Begin B: 45, End B:45, FlowRate (ml/min): 70) to give 304 (rt=1.627 min, 77.0 mg, 32%) and303 (rt=2.135 min, 111.0 mg, 46%) both as solids.

303: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.55-4.45(m, 1H), 3.20-3.10 (m, 1H), 2.16-2.04 (m, 1H), 1.95-1.59 (m, 10H),1.52-1.30 (m, 8H), 1.29-1.00 (m, 9H), 0.79 (s, 3H), 0.78-0.70 (m, 1H),0.67 (d, J=6.8 Hz, 3H), 0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H). LC-ELSD/MSpurity 99%, analytic SFC: 99.88% de. MS ESI calcd. forC₂₉H₄₂N₃[M+H—H₂O]⁺432.3, found 432.3. SFC 100% de.

304: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.76 (s, 1H), 4.30-4.20(m, 1H), 3.80-3.65 (m, 1H), 2.10-1.59 (m, 11H), 1.52-1.26 (m, 9H),1.24-1.00 (m, 8H), 0.80 (d, J=6.8 Hz, 3H), 0.78-0.72 (m, 1H), 0.71 (s,3H), 0.55-0.45 (m, 2H), 0.15-0.05 (m, 2H). LC-ELSD/MS purity 99%,analytic SFC: 97.94% de. MS ESI calcd. for C₂₉H₄₂N₃[M+H—H₂O]⁺432.3,found 432.3. SFC 100% de.

Examples 305 & 306: Synthesis of1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-hydroxy-3,10,13-trimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(305) &1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-hydroxy-3,10,13-trimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(306)

Synthesis of 305.1

To a solution of 2, 6-di-t-butyl-p-cresol (butylated hydroxytoluene)(48.0 g, 218 mmol) in toluene (150 mL) under N₂ at 0° C. was addedtrimethylaluminum (2 M in toluene, 54.5 mL, 109 mmol) dropwise. Afterstirring at 15° C. for 30 mins, a solution of 305.0 (10 g, 36.4 mmol) inDCM (100 mL) was added dropwise to the above solution under N₂ at −78°C. After stirring at −78° C. for 30 mins, MeMgBr (36.3 mL, 109 mmol, 3Min ether) was added dropwise to the reaction mixture. After stirring at−78° C. for 0.5 h, the reaction mixture was poured to ice-cooled aqueouscitric acid (400 mL) and extracted with EtOAc (2×400 mL). The combinedorganic layer was washed with brine (2×500 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜ 40% of EtAOc in PE) to give 305.1 (4.1 g, 39.0%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.52 (t, J=9.2 Hz, 1H), 2.20-2.13 (m, 1H),2.11 (s, 3H), 2.05-1.80 (m, 4H), 1.76-1.40 (m, 12H), 1.26 (s, 3H),1.25-1.01 (m, 6H), 0.94 (s, 3H), 0.59 (s, 3H).

Synthesis of 305.2

To a solution of MePh₃PBr (13.1 g, 36.9 mmol) in THF (100 mL) was addedt-BuOK (4.14 g, 36.9 mmol) at 15° C. After stirring at 50° C. for 0.5 h,a solution of 305.1 (4.1 g, 12.3 mmol) in THF (10 mL) was added into thereaction mixture below 50° C. After stirring at 60° C. for 1 h, themixture was added into NH₄Cl (100 mL, sat.) and extracted with EtOAc(2×100 mL). The combined organic layer was washed with brine (2×200 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue wastriturated from MeOH/H₂O (160 mL/160 mL) at 15° C. to give 305.2 (4.0 g,98.5%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84-4.83 (m, 1H), 4.70-4.69 (m, 1H),2.03-1.76 (m, 5H), 1.75-1.72 (m, 3H), 1.70-1.40 (m, 11H), 1.25 (s, 3H),1.23-1.00 (m, 8H), 0.94 (s, 3H), 0.54 (s, 3H).

Synthesis of 305.3

To a solution of 305.2 (2 g, 6.05 mmol) in THF (50 mL) was added BH₃Me₂S(3.02 mL, 10M, 30.2 mmol) at 15° C. After stirring for 1 h, the reactionwas sequentially treated with EtOH (50 mL) at 15° C., NaOH (24.2 mL, 5Min water, 121 mmol) at 0° C. and finally H₂O₂ (12.1 mL, 10 M in water,121 mmol) at 0° C. After stirring at 70° C. for 2 h, the resultingcolorless solution was poured into water (200 ml) and stirred for 1 h at15° C. The solid was filtered and washed with water (2×50 mL), themother liquid was quenched with aqueous Na₂S₂O₃ (250 mL, sat.). Thesolid was dissolved in DCM (100 mL) and washed with Na₂S₂O₃ (100 mlsat.), brine (100 mL), dried over anhydrous Na₂SO₄ and concentrated togive 305.3 (2 g, 95.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.79-3.58 (m, 1H), 3.51-3.31 (m, 1H),2.01-1.68 (m, 5H), 1.54-1.26 (m, 11H), 1.25 (s, 3H), 1.23-1.06 (m, 8H),1.04-0.94 (m, 6H), 0.92-0.76 (m, 2H), 0.66 (s, 3H)

Synthesis of 305.4

To a solution of 305.3 (800 mg, 2.29 mol) in DCM (10 mL) at 0° C. wereadded PPh₃ (899 mg, 3.43 mmol) and NBS (610 mg, 3.43 mmol). Afterstirring at 15° C. for 2 h, water (20 mL) was added to the resultingsolution and extracted with DCM (2×20 mL). The combined organic phasewas washed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜ 10% ofEtOAc in PE) to give 305.4 (860 mg, 91.2%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.70-3.40 (m, 1H), 3.35-3.25 (m, 1H),2.01-1.60 (m, 13H), 1.59-1.40 (m, 4H), 1.39-1.25 (m, 3H), 1.24-1.15 (m,4H), 1.09-0.95 (m, 6H), 0.94 (s, 3H), 0.90-0.68 (m, 1H), 0.67-0.64 (m,3H).

Synthesis of 305.5

To a solution of 305.4 (860 mg, 2.09 mmol) in DMF (10 mL) were addedCs₂CO₃ (1.36 g, 4.18 mmol) and 1H-pyrazole-4-carbonitrile (389 mg, 4.18mmol). After stirring at 80° C. for 20 h, the mixture was poured intoNH₄Cl (50 mL, sat.), stirred for 10 mins and filtered. The filter cakewas washed with water (2×30 ml) to give 305.5 (810 mg, 91.5%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.50-4.20 (m,1H), 3.75-3.60 (m, 1H), 2.2-2.1 (m, 1H), 1.95-1.60 (m, 12H), 1.40-1.35(m, 2H), 1.30-1.25 (m, 3H), 1.20-1.01 (m, 10H), 0.98-0.80 (m, 3H),0.79-0.70 (m, 3H), 0.69-0.65 (m, 3H).

Synthesis of 305 & 306

305.5 (810 mg, 1.91 mmol) was separated by SFC (Column: DAICEL CHIRALPAKAD-H (250 mm*30 mm, 5 um); Condition:0.1% NH₃H₂O IPA; Begin B:30%; EndB:30%) to give 305 (348.7 mg, 43.0%) and 306 (203.8 mg, 25.0%) both assolids.

305: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.48 (dd,J=3.6 Hz, 13.2 Hz, 1H), 3.66 (dd, J=10.8 Hz, 1.6 Hz, 1H), 2.20-1.57 (m,7H), 1.53-1.27 (m, 9H), 1.26 (s, 3H), 1.25-0.96 (m, 9H), 0.95 (s, 3H),0.77 (s, 3H), 0.67 (d, J=6.4 Hz, 3H). LC-ELSD/MS: purity 99%, analyticSFC: 99% de; MS ESI calcd. for C₂₇H₄₁N₃O [M−H₂O+H]⁺406.3, found 406.3.

306: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.25 (dd,J=3.6 Hz, 13.2 Hz, 1H), 3.71 (dd, J=9.2 Hz, 13.2 Hz, 1H), 2.2-1.60 (m,7H), 1.5-1.3 (m, 9H), 1.26 (s, 3H), 1.25-0.95 (m, 9H), 0.94 (s, 3H),0.81 (d, J=6.4, 3H), 0.69 (s, 3H). LC-ELSD/MS: purity 98%, analytic SFC:99% de; MS ESI calcd. for C₂₇H₄₁N₃O [M−H₂O+H]⁺406.3, found 406.3. MS ESIcalcd. for C₂₇H₄₁N₃O [M+H]⁺ 424.3, found 424.3.

Examples 307 & 308: Synthesis of1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-hydroxy-10,13-dimethyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(307) &1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17R)-3-hydroxy-10,13-dimethyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(308)

Synthesis of 307.1

To a solution of 2,6-di-t-butyl-p-cresol (butylated hydroxytoluene)(33.5 g, 152 mmol) in toluene (100 mL) under nitrogen at 0° C. was addedtrimethylaluminum (2 M in toluene, 38 mL, 76 mmol) dropwise. Afterstirring at 20° C. for 1 h, a solution of 305.0 (8.0 g, 25.2 mmol) intoluene (50 mL) was added to the above solution dropwise under N₂ at−70° C. After stirring at −70° C. for 1 h, to the resulting mixture wasadded n-PrMgCl (37.8 mL, 75.6 mmol, 2M in THF) dropwise. After stirringat −70° C. for 0.5 h, the reaction mixture was poured to ice-cooledaqueous citric acid (500 mL) and extracted with EtOAc (2×500 mL). Thecombined organic layer was washed with brine (2×300 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was combinedwith another solution of the product to be purified by flash column (0˜25% of EtOAc in PE) to give 307.1 (5.9 g, 52%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.53 (t, J=8.8 Hz, 1H), 2.19-2.13 (m, 1H),2.11 (s, 3H), 2.06-1.64 (m, 6H), 1.54-1.36 (m, 11H), 1.32-1.00 (m, 9H),0.96-0.91 (m, 6H), 0.59 (s, 3H).

Synthesis of 307.2

To a solution of PPh₃MeBr (17.4 g, 48.9 mmol) in THF (100 mL) was addedt-BuOK (5.48 g, 48.9 mmol). After stirring at 50° C. for 0.5 h, asolution of 307.1 (5.9 g, 16.3 mmol) in THF (50 mL) was added at 50° C.After stirring at 50° C. for 12 h, the mixture was poured into NH₄Cl(200 mL, sat.) and extracted with EtOAc (2×100 mL). The combined organicphase was washed with brine (2×100 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column (0˜5% of EtOAc in PE) to give 307.2 (5.2 g, 89%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H), 2.06-1.98 (m,1H), 1.92-1.80 (m, 3H), 1.75 (s, 3H), 1.73-1.46 (m, 8H), 1.45-0.97 (m,16H), 0.96-0.90 (m, 6H), 0.54 (s, 3H).

Synthesis of 307.3

To a solution of 307.2 (2.0 g, 5.57 mmol) in THF (20 mL) was addedBH₃.Me₂S (1.67 mL, 10 M, 16.7 mmol) at 0° C. After stirring at 20° C.for 16 h, the reaction mixture was sequentially treated with EtOH (5 mL)at 20° C., aq. NaOH (16.7 mL, 5 M, 83.5 mmol) and finally by H₂O₂ (8.35mL, 83.5 mmol, 10M). After stirring at 70° C. for 1 h, the reactionmixture was cooled to 20° C., poured into Na₂S₂O₃ (100 mL, sat.) andextracted with EtOAc (2×100 mL). The combined organic layer was washedwith saturated Na₂S₂O₃ (100 mL), brine (100 mL), dried over anhydrousNa₂SO₄, filtered and concentrated to give 307.3 (2.1 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.73 (dd, J=3.6, 10.8 Hz, 0.6H), 3.64 (dd,J=3.3, 10.4 Hz, 0.4H), 3.45 (dd, J=7.2, 10.8 Hz, 0.6H), 3.35 (dd, J=7.2,10.4 Hz, 0.4H), 1.98-1.54 (m, 8H), 1.45-1.18 (m, 17H), 1.17-1.01 (m,6H), 0.97-0.91 (m, 8H), 0.66 (s, 3H).

Synthesis of 307.4

To a solution of 307.3 (500 mg, 1.32 mmol) in DCM (20 mL) at 0° C. wasadded PPh₃ (519 mg, 1.98 mmol) and NBS (352 mg, 1.98 mmol). Afterstirring at 20° C. for 2 h, the reaction mixture was diluted with water(50 mL) and extracted with DCM (2×100 mL). The combined organic phasewas washed with brine (100 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜20% ofEtOAc in PE) to give 307.4 (500 mg, 86.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.62 (dd, J=3.2, 10.0 Hz, 0.6H), 3.49 (dd,J=3.2, 10.0 Hz, 0.4H), 3.40-3.30 (m, 1H), 1.98-1.50 (m, 10H), 1.50-1.15(m, 15H), 1.15-0.95 (m, 7H), 0.93-0.85 (m, 6H), 0.66 (s, 3H).

Synthesis of 307 & 308

To a solution of 307.4 (500 mg, 1.13 mmol) in DMF (20 mL) were addedCs₂CO₃ (736 mg, 2.26 mmol) and 1H-pyrazole-4-carbonitrile (210 mg, 2.26mmol). After stirring at 80° C. for 16 h, the mixture was added tosaturated NH₄Cl (100 mL) and extracted with EtOAc (3×100 mL). Thecombined organic layer was washed with LiCl (100 mL, 3% in water), brine(2×100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜30% of EtOAc in PE) to afford togive 307.5 as a solid. The residue was purified by SFC (Column: DAICELCHIRALPAK AD (250 mm*30 mm, 10 um); Condition: 0.1% NH₃H₂O IPA; Begin B:35%; End B: 35%; Flow Rate (ml/min): 60) to give 307 (181.7 mg, 35.2%)and 308 (141.8 mg, 27.6%) both as solids.

307: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.55-4.41(m, 1H), 3.73-3.57 (m, 1H), 2.17-2.04 (m, 1H), 1.93-1.78 (m, 4H),1.71-1.58 (m, 2H), 1.53-1.29 (m, 13H), 1.29-0.97 (m, 9H), 0.97-0.91 (m,6H), 0.77 (s, 3H), 0.67 (d, J=6.4 Hz, 3H). LC-ELSD/MS: purity 99%,analytic SFC: 100% de; MS ESI calcd. for C₂₉H₄₄N₃ [M−H₂O+H]⁺434.3, found434.3.

308: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.29-4.21(m 1H), 3.76-3.67 (m, 1H), 2.06-1.64 (m, 7H), 1.51-1.28 (m, 13H),1.27-0.96 (m, 9H), 0.96-0.90 (m, 6H), 0.80 (d, J=6.8 Hz, 3H), 0.69 (s,3H). LC-ELSD/MS: purity 99%, analytic SFC: 100% de; MS ESI calcd. forC₂₉H₄₄N₃ [M−H₂O+H]⁺434.3, found 434.3.

Examples 309 & 310: Synthesis of1-((R)-2-((3R,5R,8S,9S,10S,11S,13S,14S,17R)-3,11-dihydroxy-10,13-dimethyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(309) &1-((S)-2-((3R,5R,8S,9S,10S,11S,13S,14S,17R)-3,11-dihydroxy-10,13-dimethyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(310)

Synthesis of 309.1

To a solution of 2, 6-di-tert-butyl-4-methylphenol (1.03 g, 4.68 mmol)in toluene (5 mL) was added dropwise AlMe₃ (1.17 mL, 2.34 mmol, 2 M intoluene) at 0° C. under N₂. After stirring at 25° C. for 30 mins, to thefresh prepared MAD (2.34 mmol) solution under N₂ at −70° C. was added asolution of 309.0 (260 mg, 0.781 mmol) in toluene (5 mL) dropwise. Afterstirring at −70° C. for an h, chloro(propyl)magnesium (1.17 mL, 2.34mmol, 2M in THF) was added to the mixture dropwise. After stirring at−70° C. for 0.5 h, the reaction mixture was poured to ice-cooled aqueouscitric acid (20 mL) and extracted with ethyl acetate (2×30 mL). Thecombined organic layers were washed with brine (2×20 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0-60% of ethyl acetate in PE) to give 309.1 (130 mg,44.2%) as a solid. 309.1 (130 mg, 0.345 mmol) was further purified bypre-HPLC (Column: Welch Xtimate C18 150*25 mm*5 um; Condition: water(0.225% FA)-ACN; Begin B:58%; End B:88%) to afford 309.1 (45.2 mg,35.0%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.26 (br s, 1H), 2.45 (t, J=9.2 Hz, 1H),2.22-2.14 (m, 2H), 2.12 (s, 3H), 1.91-1.79 (m, 3H), 1.77-1.67 (m, 3H),1.64 (br dd, J=8.0, 3.6 Hz, 2H), 1.60 (br d, J=3.0 Hz, 1H), 1.55-1.52(m, 2H), 1.44 (br dd, J=14.4, 3.2 Hz, 1H), 1.40-1.37 (m, 1H), 1.37-1.30(m, 4H), 1.29-1.20 (m, 3H), 1.19 (s, 3H), 1.17 (br d, J=4.0 Hz, 1H),1.13 (br d, J=4.0 Hz, 1H), 1.02 (br d, J=3.6 Hz, 1H), 0.95 (t, J=7.2 Hz,3H), 0.83 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₄H₄₀O₃[M-2H₂O+H]⁺341.3 found 341.3.

Synthesis of 309.2

To a suspension of MePPh₃Br (1.37 g, 3.86 mmol) in anhydrous THF (10 mL)was added t-BuOK (433 mg, 3.86 mmol) at 25° C. under N₂. After stirringat 60° C. for 30 mins, a solution of 309.1 (730 mg, 1.93 mmol) inanhydrous THF (5 mL) was added dropwise. After stirring at 60° C. for 16h, the mixture was cooled, poured into ice-water (50 mL), stirred for 10mins, and extracted with EtOAc (2×50 mL). The combined organic phase waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtOAcin PE) to give 309.2 (540 mg) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.71 (s, 1H), 4.21 (br d,J=2.8 Hz, 1H), 2.04-1.96 (m, 2H), 1.94-1.78 (m, 4H), 1.75 (s, 3H),1.74-1.64 (m, 3H), 1.61 (br d, J=3.2 Hz, 1H), 1.57-1.79 (m, 4H),1.48-1.43 (m, 1H), 1.43-1.30 (m, 5H), 1.23-1.10 (m, 7H), 0.94 (t, J=7.2Hz, 3H), 0.79 (s, 3H).

Synthesis of 309.3

To a solution of 309.2 (540 mg, 1.44 mmol) in THF (20 mL) was addedBH₃.Me₂S (0.719 mL, 10 M, 7.19 mmol) at 25° C. After stirring at 25° C.for 16 h, the reaction was sequentially treated with EtOH (2.50 mL, 43.1mmol) at 25° C., NaOH (8.62 mL, 5.0 M, 43.1 mmol) at 0° C., and finallyby H₂O₂ (4.30 mL, 43.1 mmol, 30% in water). After stirring at 70° C. for1 h, the mixture was poured into water (100 mL) and extracted with EtOAc(2×200 mL). The combined organic layer was washed with saturated Na₂S₂O₃(200 mL), brine (200 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated to give 309.3 (460 mg) as colorless oil. The residue wasdissolved in DCM (10 mL), washed with NH₄Cl (10%, 5×50 mL), brine (2×50mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give309.3 (240 mg) as a solid.

Synthesis of 309 & 310

To a solution of 309.3 (240 mg, 0.611 mmol), Ph₃P (799 mg, 3.05 mmol)and 1H-pyrazole-4-carbonitrile (113 mg, 1.22 mmol) in DMF (10 mL) wasadded DEAD (531 mg, 0.480 mL, 3.05 mmol). After stirring at 25° C. for16 h, the mixture was poured into water (30 mL). The aqueous phase wasextracted with EtOAc (2×50 mL). The combined organic phase was washedwith brine (30 mL), dried over anhydrous Na₂SO₄, filtered, concentrated.The residue was purified by prep-HPLC (Column: Welch Xtimate C18 150*25mm*5 um; Condition: water (0.04% NH₃.H₂O)-ACN; Begin B: 60%; End B: 90%)to afford 310 (29.2 mg) as a solid and 309 (49.2 mg) as a solid.

309: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.23 (dd,J=13.6, 3.6 Hz, 1H), 4.17 (br s, 1H), 3.73 (dd, J=13.6, 9.2 Hz, 1H),2.10 (dd, J=14.0, 2.6 Hz, 1H), 2.05-1.96 (m, 1H), 1.95-1.86 (m, 2H),1.86-1.76 (m, 3H), 1.75-1.64 (m, 2H), 1.54-1.51 (m, 2H), 1.45-1.25 (m,10H), 1.22 (br d, J=6.8 Hz, 1H), 1.18 (s, 3H), 1.17-1.04 (m, 5H), 1.01(d, J=4.0 Hz, 1H), 0.96-0.92 (m, 6H), 0.83 (d, J=6.4 Hz, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₉H₄₂N₃[M-2H₂O+H]⁺432.3 found 432.3.

310: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.76 (s, 1H), 4.49 (dd,J=13.2, 4.4 Hz, 1H), 4.20 (br s, 1H), 3.67 (dd, J=13.2, 10.8 Hz, 1H),2.16-2.01 (m, 2H), 1.94-1.64 (m, 7H), 1.60 (d, J=3.2 Hz, 1H), 1.54-1.51(m, 2H), 1.46-1.25 (m, 10H), 1.22 (br d, J=5.6 Hz, 1H), 1.19 (s, 3H),1.18-1.06 (m, 5H), 1.04 (d, J=4.0 Hz, 1H), 1.01 (s, 3H), 0.99-0.89 (m,4H), 0.67 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₉H₄₂N₃[M-2H₂O+H]+432.3 found 432.3.

Examples 311 & 312: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-butyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(311)(3R,5R,8R,9R,10S,13S,14S,17R)-3-butyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(312)

Synthesis of 311.1

To the fresh prepared MAD (109 mmol) solution in toluene (500 mL) wasadded 261.1 (10 g, 36.4 mmol) in DCM (50 mL) dropwise at −70° C. Afterstirring at −70° C. for 1 h under N₂, n-BuMgCl (54.5 mL, 109 mmol, 2M)was added dropwise at −70° C. After stirring at −70° C. for another 4 h,the reaction mixture was poured into saturated aqueous citric acid (1000mL) at 10° C. and extracted with EtOAc (2×500 mL). The combined organiclayer was dried over Na₂SO₄, filtered and concentrated in vacuum to givethe product which was purified by flash column (0˜40% of EtOAc in PE) togive 311.1 (10 g, 82.6%) as a solid

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.47-2.38 (m, 1H), 2.14-2.01 (m, 1H),1.98-1.88 (m, 1H), 1.85-1.64 (m, 6H), 1.59-1.41 (m, 7H), 1.37-1.01 (m,14H), 0.96-0.89 (m, 3H), 0.86 (s, 3H).

Synthesis of 311.2

To a mixture of EtPPh₃Br (26.7 g, 72.0 mmol) in THF (120 mL) was addedt-BuOK (8.07 g, 72.0 mmol) at 25° C. under N₂. After stirring at 40° C.for 30 mins, 311.1 (12.0 g, 36.0 mmol) was added at 40° C. Afterstirring at 40° C. for 3 h, the reaction mixture was quenched withsaturated NH₄Cl aqueous (200 mL) at 25° C. and extracted with EtOAc(2×100 mL). The combined organic phase was concentrated under vacuum togive a solid, which was purified by trituration with MeOH/H₂O (1:1, 150mL) to give 311.2 (12 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.22-5.03 (m, 1H), 2.42-2.12 (m, 3H),1.85-1.68 (m, 4H), 1.67-1.62 (m, 4H), 1.57-1.42 (m, 8H), 1.36-1.07 (m,14H), 0.92 (t, J=6.8 Hz, 3H), 0.87 (s, 3H)

Synthesis of 311.3

To a solution of 311.2 (12 g, 34.8 mmol) in THF (150 mL) under N₂ wasadded 9-BBN dimer (16.9 g, 69.6 mmol) at 15° C. After stirring at 45° C.for 16 h, the resulting mixture was treated sequentially with ethanol(19.8 mL, 347 mmol) at 15° C., NaOH aqueous (69.4 mL, 5.0 M, 347 mmol)at 0° C. and then hydrogen peroxide (34.7 mL, 10 M, 347 mmol) dropwiseat 15° C. After stirring at 70° C. for 1 h, the reaction was cooled to15° C. and added into the water (200 mL) and saturated aqueous Na₂S₂O₃(200 mL). The aqueous phase was extracted with EtOAc (2×200 mL). Thecombined organic phase was washed with brine (200 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give the product, whichwas purified by trituration with H₂O (250 mL) at 20° C. to give 311.3(15.1 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.81-3.53 (m, 1H), 1.97-1.59 (m, 1H),1.97-1.58 (m, 9H), 1.51-1.25 (m, 15H), 1.23-1.03 (m, 11H), 0.92 (t,J=6.8 Hz, 3H), 0.66 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₄H₃₉ [M-2H₂O+H]⁺327.3, found 327.3.

Synthesis of 311.4

To a solution of 311.3 (15 g, 41.3 mmol) in DCM (200 mL) at 0° C. wasadded silica gel (18 g) and PCC (17.7 g, 82.6 mmol). After stirring at20° C. for 3 h, PE (100 mL) was added to the reaction mixture. Theresulting mixture was filtered through a pad of silica gel and thefilter cake was washed with DCM (400 mL). The filtrate was concentratedand the residue was purified by flash column (0˜30% of EtOAc in PE) togive 311.4 (10 g, 67.5%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.54 (t, J=8.8 Hz, 1H), 2.20-2.13 (m, 1H),2.12 (s, 3H), 2.03-1.97 (m, 1H), 1.83-1.60 (m, 7H), 1.49-1.26 (m, 15H),1.25-1.04 (m, 6H), 0.92 (t, J=6.4 Hz, 3H), 0.61 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₄H₃₉O [M−H₂O+H]+343.3, found 343.3.

Synthesis of 311.5

To a mixture of MePPh₃Br (5.93 g, 16.6 mmol) in THF (30 mL) was addedt-BuOK (1.86 g, 16.6 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 mins, 311.4 (3 g, 8.31 mmol) was added at 50° C. After stirringat 50° C. for 16 h, the reaction mixture was quenched with saturatedNH₄Cl aqueous (100 mL) at 25° C. and extracted with EtOAc (2×100 mL).The combined organic phase was concentrated under vacuum to give asolid, which was purified by flash column (0˜30% of EtOAc in PE) to give311.5 (1.2 g, 40.2%) as a solid

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.05 (s, 2H),1.85-1.57 (s, 13H), 1.50-1.20 (m, 16H), 1.18-1.02 (m, 4H), 0.92 (t,J=6.4 Hz, 3H), 0.57 (s, 3H).

Synthesis of 311.6

To a solution of 311.5 (1.2 g, 3.34 mmol) in THF (10 mL) was addedBH₃Me₂S (1.67 mL, 10 M, 16.7 mmol). After stirring at 25° C. for 16 hunder N₂, the reaction mixture was sequentially treated with EtOH (1.91mL, 33.4 mmol), NaOH (1.33 g in 6.68 mL water, 33.4 mmol) and H₂O₂ (3.34mL, 10 M, 33.4 mmol) dropwise. After stirring at 70° C. for 2 h, thereaction mixture was quenched by Na₂SO₃ (100 mL, 10%) and extracted withEtOAc (2×200 mL). The combined organic layer was dried over Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜30% of EtOAc in PE) to give 311.6 (1.2 g, 96.0%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.79-3.59 (m, 1H), 3.50-3.32 (m, 1H),1.99-1.59 (m, 8H), 1.50-1.14 (m, 18H), 1.11-1.01 (m, 5H), 0.96 (s, 1H),0.94 (s, 1H), 0.92 (t, J=6.4 Hz, 3H), 0.68 (s, 3H).

Synthesis of 311.7

To a solution of 311.6 (300 mg, 0.8 mmol) in DCM (5 mL) at 0° C. wasadded PPh₃ (312 mg, 1.2 mmol) and NBS (211 mg, 1.2 mmol). After stirringat 20° C. for 2 h, the reaction was diluted with water (20 mL) andextracted with DCM (2×20 mL). The combined organic phase was washed withbrine (20 mL), dried over anhydrous Na₂SO₄, filtered and concentrated.The residue was purified by flash column (0˜15% of EtOAc in PE) to give311.7 (270 mg, 77.1%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.42-3.29 (m, 1H), 1.96-1.42 (m, 15H),1.36-1.23 (m, 13H), 1.14-0.98 (m, 8H), 0.95-0.89 (m, 3H), 0.70-0.65 (m,3H).

Synthesis of 311.8

To a solution of 311.7 (300 mg, 0.7 mmol) in DMF (5 mL) were addedCs₂CO₃ (443 mg, 1.4 mmol) and 1H-pyrazole-4-carbonitrile (126 mg, 1.4mmol). After stirring at 80° C. for 16 h under N₂, the mixture was addedinto saturated NH₄Cl (20 mL) and extracted with EtOAc (3×50 mL). Thecombined organic layer was washed with LiCl (50 mL, 3% in water), brine(2×100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜30% of EtOAc in PE) to give311.8 (200 mg, 72.2%) as a solid.

Separation of 311 & 312

The diastereomeric mixture 311.8 (400 mg, 0.9 mmol) was separated by SFC(Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, 5 um); Condition: 0.1%NH₃H₂O ETOH; Begin B: 40%; End B: 40%; Flow Rate (ml/min): 60) to give311 (86.5 mg, 21.6%) and 312 (58.2 mg, 14.5%) both as solids.

311: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.50 (dd,J=4.4, 13.6 Hz, 1H), 3.70-3.60 (m, 1H), 2.18-2.05 (m, 1H), 1.91-1.57 (m,9H), 1.49-1.25 (m, 14H), 1.24-1.03 (m, 7H), 0.92 (t, J=6.4 Hz, 3H), 0.79(s, 3H), 0.68 (d, J=6.8 Hz, 3H). LC-ELSD/MS purity 99%, analytic SFC:100% de, MS ESI calcd. for C₂₉H₄₄N₃ [M−H₂O+H]⁺434.3, found 434.3. SFC100% de.

312: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.26 (dd,J=4.0, 13.6 Hz, 1H), 3.76-3.67 (m, 1H), 2.08-1.88 (m, 3H), 1.83-1.57 (m,6H), 1.50-1.21 (m, 15H), 1.20-0.99 (m, 7H), 0.92 (t, J=6.8 Hz, 3H), 0.81(d, J=6.8 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, analytic SFC:100% de, MS ESI calcd. for C₂₉H₄₄N₃ [M−H₂O+H]⁺434.3, found 434.3. SFC100% de.

Examples 313-316: Synthesis of(3R,5R,8R,9S,10S,13S,14S,17R)-10,13-dimethyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(313) &(3R,5R,8R,9S,10S,13S,14S,17R)-10,13-dimethyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(314) &(3R,5R,8R,9S,10S,13S,14S,17R)-10,13-dimethyl-17-((R)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(315) &(3R,5R,8R,9S,10S,13S,14S,17R)-10,13-dimethyl-17-((S)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(316)

Synthesis of 313.1

To a solution of 307.2 (1.5 g, 4.2 mmol) in THF (15 mL) was addedBH₃.Me₂S (2.09 mL, 20.9 mmol, 10 M) dropwise at 0° C. After stirring at25° C. for 3 h, the reaction mixture was cooled to 0° C. andsequentially treated with ethanol (1.92 g, 41.8 mmol) dropwise at 0° C.NaOH aqueous (6.26 mL, 62.6 mmol, 10 M) dropwise followed by H₂O₂ (6.26mL, 62.6 mmol) at 0° C. After stirring at 70° C. for 1 h, the mixturewas extracted with EtOAc (2×25 mL). The combined organic phase waswashed with saturated Na₂S₂O₃ aqueous (2×30 mL), brine (30 mL), driedover Na₂SO₄, filtered and evaporated to give 313.1 (1.8 g) as solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.76-3.69 (m, 1H), 3.50-3.32 (m, 1H),1.94-1.75 (m, 4H), 1.72-1.65 (m, 2H), 1.60-1.29 (m, 11H), 1.28-1.14 (m,9H), 1.13-1.00 (m, 5H), 0.99-0.85 (m, 8H), 0.66 (s, 3H).

Synthesis of 313.2

To a solution of 313.1 (1.8 g, 4.77 mmol) in DCM (20 mL) was added PPh₃(1.87 g, 7.15 mmol) and NBS (1.27 g, 7.15 mmol) at 0° C. After stirringat 25° C. for 2 h, the reaction was added to water (20 mL) and extractedwith DCM (2×20 mL). The combined organic phase was washed with brine (20mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜25% of EtOAc in PE) to give 313.2 (1 g,47.8%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.65-3.50 (m, 1H), 3.40-3.33 (m, 1H),1.90-1.76 (m, 4H), 1.75-1.65 (m, 2H), 1.64-1.51 (m, 8H), 1.49-1.21 (m,10H), 1.19-1.01 (m, 6H), 1.00-0.84 (m, 8H), 0.66 (s, 3H).

Synthesis of 313.3 & 315.1

To a solution of 313.2 (1 g, 2.27 mmol) in DMF (8 mL) were added Cs₂CO₃(1.47 g, 4.54 mmol) and 5-methyl-2H-1,2,3,4-tetrazole (381 mg, 4.54mmol). After stirring at 80° C. for 16 h, the mixture was added intosaturated NH₄Cl (20 mL) and extracted with EtOAc (3×20 mL). The combinedorganic layer was washed with water (2×20 mL), brine (20 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜100% of EtOAc in PE) to give 313.3 (735 mg) and 315.1(287 mg) both as colorless oil.

313.3: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.77-4.50 (m, 1H), 4.32-4.23 (m,1H), 2.53 (m, 3H), 2.26-2.08 (m, 1H), 2.03-1.78 (m, 4H), 1.71-1.62 (m,2H), 1.56-1.08 (m, 9H), 1.06-1.02 (m, 9H), 1.00-0.90 (m, 8H), 0.89-0.80(m, 5H), 0.75-0.58 (s, 3H). 315.1: ¹H NMR (400 MHz, CDCl₃) δ_(H)4.56-4.50 (m, 1H), 4.32-3.75 (m, 1H), 2.95 (s, 3H), 2.88 (s, 3H), 2.54(s, 3H), 2.28-2.16 (m, 1H), 1.93-1.79 (m, 4H), 1.73-1.31 (m, 10H),1.28-1.01 (m, 9H), 1.00-0.90 (m, 5H), 0.89-0.80 (m, 3H), 0.75-0.58 (s,3H).

Separation of 313 & 314

313.3 (735 mg) was separated by SFC (DAICEL CHIRALCEL OD-H (250 mm*30mm, 5 um), Condition: 0.1% NH₃H₂O ETOH, Begin B:40%, End B:40%,FlowRate(ml/min):50) to afford 313 (352.1 mg, 47.9%) and 314 (202.6 mg, 27.5%)both as solids.

313: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.56-4.50 (m, 1H), 4.32-4.23 (m, 1H),2.53 (m, 3H), 2.20-2.08 (m, 1H), 2.03-1.78 (m, 4H), 1.71-1.62 (m, 2H),1.56-1.08 (m, 11H), 1.06-1.02 (m, 11H), 1.00-0.90 (m, 6H), 0.89-0.80 (m,3H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₇H₄₅N₄[M−H₂O+H]⁺425 found 425. SFC 100% de.

314: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.56-4.50 (m, 1H), 4.32-4.24 (m, 1H),2.53 (s, 3H), 2.28-2.16 (m, 1H), 1.93-1.79 (m, 4H), 1.73-1.58 (m, 2H),1.57-1.31 (m, 11H), 1.28-1.01 (m, 11H), 1.00-0.90 (m, 6H), 0.89-0.80 (m,3H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₇H₄₅N₄[M−H₂O+H]⁺425 found 425. SFC 100% de.

Separation of 315 & 316

315.1 (287 mg) was separated by SFC (DAICEL CHIRALCEL OD-H (250 mm*30mm, 5 um), Condition: 0.1% NH₃H₂O ETOH, Begin B:35%, End B:35%, FlowRate(ml/min):50) to afford 315 (83.7 mg, 29.2%) and 316 (67.8 mg, 23.7%)both as solids.

315: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.32-4.27 (m, 1H), 3.90-3.80 (m, 1H),2.55 (s, 3H), 2.20-1.80 (m, 5H), 1.78-1.59 (m, 2H), 1.50-1.25 (m, 12H),1.24-1.05 (m, 10H), 1.02-0.95 (m, 6H), 0.82 (d, J=6.5 Hz, 3H), 0.70 (s,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₅N₄ [M−H₂O+H]⁺425found 425. SFC 100% de.

316: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.56-4.49 (m, 1H), 3.90-3.79 (m, 1H),2.55 (s, 3H), 2.19-2.06 (m, 1H), 1.94-1.79 (m, 4H), 1.73-1.60 (m, 2H),1.51-1.32 (m, 13H), 1.28-0.98 (m, 9H), 0.97-0.91 (m, 6H), 0.80 (s, 3H),0.73-0.67 (m, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₅N₄[M−H₂O+H]⁺425 found 425. SFC 100% de.

Examples 317 & 318: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-13-ethyl-3-hydroxy-3-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(317) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-13-ethyl-3-hydroxy-3-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(318)

Synthesis of 317.1

To a mixture of 317.0 (4.0 g, 13.9 mmol) and Pd/C (1.0 g, 10% Palladiumon carbon, 50% water wet) in THF (40 mL) was added hydrobromic acid (1mL, 40% in water) at 25° C. After hydrogenating under 15 psi of hydrogenfor 16 h, the mixture was filtered through a pad of celite andconcentrated to afford 317.1 (5.1 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.58 (t, J=14.4 Hz, 1H), 2.49-2.36 (m,1H), 2.30-2.03 (m, 7H), 1.97-1.88 (m, 1H), 1.82-1.59 (m, 7H), 1.55-1.11(m, 8H), 0.79 (t, J=7.6 Hz, 3H).

Synthesis of 317.2

To a MAD (207 mmol in 100 mL toluene) solution was added a solution of317.1 (10 g, 34.6 mmol) in DCM (50 mL) dropwise at −70° C. Afterstirring at −70° C. for 1 h under N₂, MeMgBr (34.3 mL, 103 mmol, 3M inethyl ether) was added dropwise at −70° C. After stirring at −70° C. foranother 2 h, the reaction mixture was poured into saturated aqueouscitric acid (400 mL) at 10° C. and extracted with EtOAc (2×200 mL). Thecombined organic layer was dried over Na₂SO₄, filtered and concentrated.The residue was purified by flash column (0˜35% of EtOAc in PE) toafford 317.2 (6.05 g, 58%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.40 (dd, J=19.2, 8.8 Hz, 1H), 2.14-1.99(m, 2H), 1.93-1.83 (m, 4H), 1.78-1.60 (m, 3H), 1.58-1.48 (m, 3H),1.47-1.37 (m, 5H), 1.36-1.23 (m, 8H), 1.20-1.05 (m, 2H), 1.03-0.91 (m,1H), 0.76 (t, J=7.2 Hz, 3H).

Synthesis of 317.3

To a mixture of EtPPh₃Br (43.8 g, 118 mmol) in THF (170 mL) was addedt-BuOK (13.2 g, 118 mmol) at 20° C. under N₂. After stirring at 40° C.for 1 h, a solution of 317.2 (6.05 g, 19.8 mmol) in THF (20 mL) wasadded at 40° C. After stirring at 40° C. for 36 h, the reaction mixturewas quenched with 10% NH₄Cl aqueous (100 mL) at 20° C. and extractedwith EtOAc (2×100 mL). The combined organic phase was washed with brine(20 mL) dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜12% of EtOAc in PE) to give317.3 (5.2 g, 83%) as oil

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.23-5.15 (m, 1H), 2.46-2.30 (m, 2H),2.22-2.08 (m, 1H), 1.90-1.78 (m, 3H), 1.71-1.63 (m, 5H), 1.56-1.40 (m,7H), 1.34-1.22 (m, 9H), 1.20-0.92 (m, 5H), 0.83 (t, J=7.2 Hz, 3H).

Synthesis of 317.4

To a solution of 317.3 (4.2 g, 3.15 mmol) in THF (60 mL) was addedBH₃Me₂S (3.95 mL, 39.5 mmol, 10M) at 0° C. After stirring at 20° C. for12 h, the reaction mixture was sequentially treated with ethanol (13.8mL) at 15° C., NaOH aqueous (47.7 mL, 5.0 M) at 0° C. and finallyhydrogen peroxide (23.7, 10 M) dropwise at 0° C. After stirring at 78°C. for 1 h, the reaction mixture was cooled to 15° C. The aqueous phasewas collected and treated with saturated aqueous Na₂S₂O₃ (80 mL). Theaqueous phase was extracted with EtOAc (2×100 mL). The combined organicphase was washed with brine (2×30 mL), dried over anhydrous Na₂SO₄,filtered and concentrated to give 317.4 (4.6 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.88-3.69 (m, 1H), 2.32-2.09 (m, 1H),1.93-1.75 (m, 4H), 1.71-1.61 (m, 5H), 1.50-1.24 (m, 17H), 1.18-1.03 (m,5H), 0.99-0.80 (m, 5H).

Synthesis of 317.5

To a solution of 317.4 (5.6 g, 16.7 mmol) in DCM (200 mL) at 10° C. wasadded DMP (14.1 g, 33.4 mmol). After stirring at 20° C. for 30 minutes,the mixture was quenched by saturated NaHCO₃ aqueous (50 mL) andsaturated Na₂S₂O₃ (50 mL) at 10° C. The DCM phase was separated andwashed with saturated NaHCO₃/Na₂S₂O₃ aqueous (1:1, 2×30 mL), brine (20mL), dried over Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜25% of EtOAc in PE) to give 317.5 (4.15 g,75%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.50-2.42 (m, 1H), 2.34-2.17 (m, 5H),1.89-1.78 (m, 3H), 1.75-1.58 (m, 4H), 1.50-1.36 (m, 6H), 1.33-1.03 (m,13H), 0.96-0.81 (m, 1H), 0.62 (t, J=7.6 Hz, 3H).

Synthesis of 317.6

To a mixture of MePPh₃Br (17.7 g, 49.6 mmol) in THF (80 mL) was addedt-BuOK (5.55 g, 49.6 mmol) at 20° C. under N₂. After stirring at 50° C.for 1 h, a solution of 317.5 (4.15 g, 12.4 mmol) in THF (10 mL) wasadded at 50° C. After stirring at 50° C. for 12 h, the reaction mixturewas quenched with 10% NH₄Cl aqueous (50 mL) at 20° C. and extracted withEtOAc (2×80 mL). The combined organic phase was washed with brine (30mL) dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜12% of EtOAc in PE) to give 317.6 (2.85g, 70%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.80 (s, 1H), 4.77 (s, 1H), 2.24-2.19 (m,1H), 1.99-1.94 (m, 1H), 1.92-1.80 (m, 6H), 1.66-1.57 (m, 4H), 1.50-1.33(m, 7H), 1.30-1.23 (m, 7H), 1.19-0.97 (m, 6H), 0.91-0.81 (m, 1H), 0.74(t, J=7.6 Hz, 3H).

Synthesis of 317.7

To a solution of 317.6 (1.0 g, 3.02 mmol) in THF (10 mL) was addedBH₃Me₂S (1.20 mL, 12.0 mmol, 10 M) at 0° C. After stirring at 20° C. for12 h, the resulting mixture was treated sequentially with ethanol (3.16mL) at 15° C., NaOH aqueous (10.8 mL, 5.0 M) at 0° C., and finallyhydrogen peroxide (5.43 mL, 10 M) dropwise at 0° C. After stirring at78° C. for 1 h, the mixture was cooled to 15° C. and quenched withsaturated aqueous Na₂S₂O₃ (30 mL). The aqueous phase was extracted withEtOAc (2×60 mL). The combined organic phase was washed with brine (2×30mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜30% of EtOAc in PE) to give 317.7 (1.0g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.88-3.60 (m, 1H), 3.45-3.33 (m, 1H),2.28-2.12 (m, 1H), 1.89-1.70 (m, 4H), 1.68-1.56 (m, 4H), 1.50-1.16 (m,17H), 1.14-1.02 (m, 5H), 0.99-0.82 (m, 7H)

Synthesis of 317.8

To a solution of 317.7 (500 mg, 1.43 mmol) in DCM (10 mL) was addedtriphenylphosphine (561 mg, 2.14 mmol) and NBS (380 mg, 2.14 mmol) at 0°C. under N₂. After stirring at 15° C. for 2 h. The mixture wasconcentrated. The residue was purified by flash column (0˜13% of EtOAcin PE) to give 317.8 (300 mg, 51%) as a solid.

¹H NMR (400 MHz, CDCl₃) 3.75-3.48 (m, 1H), 3.40-3.31 (m, 1H), 2.25-2.12(m, 1H), 1.93-1.76 (m, 5H), 1.68-1.59 (m, 2H), 1.50-1.22 (m, 16H),1.20-0.99 (m, 9H), 0.94-0.83 (m, 4H).

Synthesis of 317.9

To a solution of 317.8 (360 mg, 0.87 mmol) in DMF (8 mL) were added1H-pyrazole-4-carbonitrile (161 mg, 1.74 mmol) and Cs₂CO₃ (566 mg, 1.74mmol) at 20° C. under N₂. After stirring at 80° C. for 12 h, the mixturewas diluted with H₂O (15 mL) and extracted with EtOAc (3×30 mL). Thecombined organic phase was washed with H₂O (2×30 mL) and brine (30 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜25% of EtOAc in PE) to give 317.9 (300 mg)as a solid.

¹H NMR (400 MHz, CDCl₃) 7.80 (s, 1H), 7.75 (s, 1H), 4.65-4.26 (m, 1H),3.72-3.60 (m, 1H), 2.30-2.12 (m, 2H), 1.90-1.75 (m, 4H), 1.70-1.57 (m,3H), 1.51-1.35 (m, 8H), 1.32-1.20 (m, 8H), 1.14-1.01 (m, 6H), 0.98-0.80(m, 4H), 0.71-0.63 (m, 2H).

Separation of 317 & 318

317.9 (300 mg) was separated by SFC (column: DuraShell 150*25 mm*5 um,A; CO₂; B: 0.1% NH₃H₂O EtOH; gradient: 25-25%, flow rate: 60 mL/min.) togive 317 (89.3 mg, 30%) as a solid and 318 (118.0 mg, 39%) as a solid.

317: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.60 (dd,J=13.6, 4.0 Hz, 1H), 3.67 (dd, J=13.6, 10.4 Hz, 1H), 2.31-2.15 (m, 2H),1.88-1.75 (m, 4H), 1.70-1.56 (m, 3H), 1.52-1.35 (m, 8H), 1.33-1.16 (m,8H), 1.14-0.84 (m, 9H), 0.68 (d, J=6.8 Hz, 3H). LC-ELSD/MS: purity 99%,analytic SFC: 99.78% de; MS ESI calcd. for C₂₇H₄₀N₃ [M−H₂O+H]+406.3,found 406.3; MS ESI calcd. for C₂₇H₄₂N₃O [M+H]⁺ 424.3, found 424.3. SFC100% de.

318: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.29 (dd,J=13.2, 4.0 Hz, 1H), 3.67 (dd, J=13.2, 9.6 Hz, 1H), 2.27-2.10 (m, 2H),1.94-1.75 (m, 4H), 1.68-1.57 (m, 3H), 1.50-1.32 (m, 8H), 1.30-0.95 (m,13H), 0.93-0.81 (m, 7H). LC-ELSD/MS: purity 99%, analytic SFC: 99.36%de; MS ESI calcd. for C₂₇H₄₀N₃[M−H₂O+H]⁺ 406.3, found 406.3. SFC 100%de.

Examples 319-322: Synthesis of(3R,5R,8S,9S,10R,13S,14S,17R)-10-(methoxymethyl)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(319) &(3R,5R,8S,9S,10R,13S,14S,17R)-10-(methoxymethyl)-13-methyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(320) &(3R,5R,8S,9S,10R,13S,14S,17R)-10-(methoxymethyl)-13-methyl-17-((R)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(321) &(3R,5R,8S,9S,10R,13S,14S,17R)-10-(methoxymethyl)-13-methyl-17-((S)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(322)

Synthesis of 319.1

To a solution of 319.0 (30 g, 98.5 mmol) in THF (200 mL) was added KOH(16.5 g, 295 mmol) and Me₂SO₄ (25.2 g, 197 mmol) at 0° C. After stirringat 25° C. for 16 h under N₂, the mixture was quenched with water (300mL) and extracted with EtOAc (3×500 mL). The combined organic layerswere washed with brine (1 L), dried over anhydrous Na₂SO₄, filtered andevaporated. The residue was purified by flash column (0˜ 15% of EtOAc inPE) to afford 319.1 (21.4 g, 68%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.61-3.58 (d, J=9.2 Hz, 3H), 3.35-3.33 (m,4H), 2.66-2.58 (m, 1H), 2.50-2.43 (m, 1H), 2.37-1.81 (m, 11H), 1.77-1.05(m, 7H), 0.89 (s, 3H).

Synthesis of 319.2

To a solution of 2,6-di-tert-butyl-4-methylphenol (42.7 g, 194 mmol) intoluene (70 mL) was added dropwise AlMe₃ (48.5 mL, 97.1 mmol, 2 M intoluene) at 0° C. After stirring at 25° C. for 30 mins, to the MADsolution was added a solution of 319.1 (10 g, 31.4 mmol) in anhydroustoluene (200 mL) dropwise at −70° C. After stirring at −70° C. for 1 hunder N₂, n-PrMgCl (47 mL, 94.1 mmol, 2 M in diethyl ether) was addeddropwise at −70° C. After stirring at −70° C. for another 2 h, thereaction mixture was poured into saturated aqueous citric acid (500 mL)at 0° C. and extracted with EtOAc (2×500 mL). The combined organic layerwas dried over anhydrous Na₂SO₄, filtered and concentrated in vacuum.The residue was purified by flash column (0˜ 20% of EtOAc in PE) to give319.2 (7.4 g, 65%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.55-3.53 (d, J=9.2 Hz, 1H), 3.33 (s, 3H),3.22-3.20 (d, J=9.2 Hz, 1H), 2.47-2.40 (m, 1H), 2.12-2.03 (m, 1H),1.96-1.61 (m, 7H), 1.54-1.39 (m, 9H), 1.37-1.15 (m, 9H), 0.93 (t, J=7.2Hz, 3H), 0.85 (s, 3H).

Synthesis of 319.3

To a suspension of EtPPh₃Br (30.2 g, 81.6 mmol) in THF (150 mL) wasadded t-BuOK (9.15 g, 81.6 mmol) at 15° C. under N₂. After stirring at45° C. for 30 mins, 319.2 (7.4 g, 20.4 mmol) in THF (50 mL) was added.After stirring at 45° C. for 16 h, the resulting suspension was pouredinto water (200 mL) and extracted with EtOAc (3×250 mL). The combinedorganic layer was concentrated. The residue was purified by column (0˜5% of EtOAc in PE) to give 319.3 (8.4 g) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.13-5.07 (m, 1H), 3.58-3.55 (d, J=9.2 Hz,1H), 3.33 (s, 3H), 3.20-3.18 (d, J=9.2 Hz, 1H), 2.38-2.12 (m, 3H),1.90-1.67 (m, 4H), 1.64-1.54 (m, 6H), 1.48-1.22 (m, 13H), 1.21-1.08 (m,4H), 0.93 (t, J=7.2 Hz, 3H), 0.85 (s, 3H).

Synthesis of 319.4

To a solution of 319.3 (8.4 g, 22.4 mmol) in THF (150 mL) was added9-BBN dimmer (21.8 g, 89.6 mmol) at 25° C. under N₂. After stirring at40° C. for 16 h, the reaction mixture was cooled and quenched with EtOH(20.9 g, 448 mmol) at 0° C., followed by adding NaOH (89.6 mL, 5 M, 448mmol) very slowly. After addition, H₂O₂ (53.7 mL, 537 mmol, 10 M inwater) was added slowly until the reaction temperature no longer risesand the reaction temperature was maintained below 30° C. After stirringat 80° C. for another 2 h, the aqueous phase was extracted with EtOAc(2×350 mL). The combined organic phase was washed with saturated Na₂S₂O₃(2×100 mL), brine (150 mL), drive over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by column (0˜ 20% of EtOAc in PE)to give 319.4 (7.86 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.72-3.65 (m, 1H), 3.56-3.54 (d, J=9.2 Hz,1H), 3.32 (s, 3H), 3.18-3.16 (d, J=9.2 Hz, 1H), 1.95-1.72 (m, 5H),1.70-1.49 (m, 5H), 1.48-1.23 (m, 13H), 1.21-1.04 (m, 9H), 0.93 (t, J=6.8Hz, 3H), 0.64 (s, 3H).

Synthesis of 319.5

To a solution of 319.4 (7.36 g, 18.7 mmol) in DCM (150 mL) was added DMP(15.8 g, 37.4 mmol). After stirring at 25° C. for 1 h, the mixture wasquenched with NaHCO₃ (300 mL) and Na₂S₂O₃ (300 mL). The mixture wasextracted with DCM (2×300 mL) and the organic layer was washed withNa₂S₂O₃ (2×100 mL, sat.), brine (300 mL, sat.), dried over Na₂SO₄,filtered and concentrated in vacuum to give 319.5 (11 g) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.54-3.52 (d, J=9.2 Hz, 1H), 3.32 (s, 3H),3.19-3.17 (d, J=9.2 Hz, 1H), 2.52 (t, J=8.8 Hz, 1H), 2.26-1.63 (m, 15H),1.49-1.08 (m, 15H), 0.93 (t, J=7.2 Hz, 3H), 0.59 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₅H₄₁O₂[M−H₂O+H]+373.3, found 373.3,C₂₄H₃₇₀ [M-MeOH—H₂O+H]⁺341.3, found 341.3.

Synthesis of 319.6

To a suspension of MePh₃BrP (14.6 g, 40.9 mmol) in anhydrous THF (100mL) was added t-BuOK (4.58 g, 40.9 mmol) at 25° C. under N₂. Afterstirring at 60° C. for 30 mins, a solution of 319.5 (3.2 g, 8.19 mmol)in anhydrous THF (30 mL) was added dropwise at 25° C. After stirring at60° C. for 16 h, the resulting suspension was poured into saturatedNH₄Cl (100 mL) and extracted with EtOAc (2×100 mL). The combined organicphase was washed with brine (200 mL), filtered and concentrated. Theresidue was purified by flash column (0˜ 5% of EtOAc in PE) to give319.6 (2.8 g) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H), 3.58-3.56 (d,J=8.8 Hz, 1H), 3.33 (s, 3H), 3.18-3.16 (d, J=9.2 Hz, 1H), 2.04-1.99 (m,1H), 1.87-1.65 (m, 7H), 1.59-1.37 (m, 6H), 1.36-1.02 (m, 13H), 0.93 (t,J=6.8 Hz, 3H), 0.89-0.83 (m, 4H), 0.54 (s, 3H).

Synthesis of 319.7

To a solution of 319.6 (1 g, 2.57 mmol) in THF (10 ml) was addedBH₃.Me₂S (2.05 mL, 20.5 mmol, 10M) at 0° C. After stirring at 15° C. for16 h, the reaction mixture was cooled and sequentially treated with EtOH(718 mg, 51.3 mmol) at 0° C., NaOH (10.2 mL, 51.3 mmol, 5M) very slowlyand finally H₂O₂ (6.15 mL, 61.6 mmol) slowly until the reactiontemperature no longer rises and the reaction temperature was maintainedbelow 30° C. After stirring at 80° C. for another 1 h, saturated aqueousNa₂S₂O₃ (50 mL) was added and the mixture was stirred at 0° C. foranother 1 h. The aqueous phase was extracted with EtOAc (3×50 mL). Thecombined organic phase was washed with saturated Na₂S₂O₃ (100 mL), brine(100 mL), drive over anhydrous Na₂SO₄, filtered and concentrated to give319.7 (800 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.60 (m, 1H), 3.57-3.55 (m, 1H),3.46-3.36 (m, 1H), 3.33 (s, 3H), 3.18-3.16 (d, J=8.8 Hz, 1H), 1.97-1.58(m, 7H), 1.52-1.27 (m, 9H), 1.25-1.02 (m, 12H), 0.95-0.73 (m, 8H), 0.66(s, 3H).

Synthesis of 319.8 & 319.9

To a solution of 319.7 (400 mg, 0.983 mmol) and5-methyl-2H-1,2,3,4-tetrazole (123 mg, 1.47 mmol) in THF (5 mL) wereadded Ph₃P (411 mg, 1.57 mmol) and DEAD (273 mg, 1.57 mmol) at 0° C.After stirring at 25° C. for 16 h, the mixture was poured into ice-water(50 mL) and extracted with EtOAc (3×50 mL). The combined organic phasewas washed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜ 50% ofEtOAc in PE) to give 319.8 (350 mg) and 319.9 (100 mg) both as oils.

Separation of 319 & 320

319.8 (350 mgl) was separated by SFC (Column: DAICEL CHIRALPAK AD (250mm*30 mm, 10 um); Condition: 0.1% NH₃H₂O IPA; Begin B: 40%; End B: 40%)to give 319 (Peak 2, 149.9 mg, Rt=2.912 min, 43%) and 320 (Peak 1, 121.3mg, Rt=1.369 min, 35%) both as solids.

319.9 (100 mg) was further purified by HPLC (Column: Welch Xtimate C18150*25 mm*5 um; Condition: water (0.05% NH₃H₂O)-ACN; Begin B: 70; End B:100; Gradient Time(min): 8.5; 100% B Hold Time(min): 2;FlowRate(ml/min): 30; Injections: 8) to give 50 mg as a solid, which wasseparated by SFC (Column: DAICEL CHIRALPAK AD (250 mm*30 mm, 10 um);Condition: 0.1% NH₃H₂O ETOH; Begin B: 30%; End B: 30%) to give 321 (Peak1, 8.2 mg, Rt=1.443 min, 16%) and 322 (Peak 2, 15.1 mg, Rt=1.638 min,30%) both as solids.

319: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.75-4.70 (m, 1H), 4.26-4.20 (m, 1H),3.57-3.54 (d, J=9.2 Hz, 1H), 3.33 (s, 3H), 3.20-3.18 (d, J=8.8 Hz, 1H),2.53 (s, 3H), 2.25-2.17 (m, 1H), 1.91-1.58 (m, 10H), 1.53-1.26 (m, 14H),1.24-1.06 (m, 6H), 0.93 (t, J=6.8 Hz, 3H), 0.79 (s, 3H), 0.71-0.70 (d,J=6.4 Hz, 1H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₇O[M−H₂O+H]⁺455.4, found 455.4. SFC 100% de.

320: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.54-4.50 (m, 1H), 4.31-4.25 (m, 1H),3.56-3.54 (d, J=9.2 Hz, 1H), 3.30 (s, 3H), 3.18-3.16 (d, J=8.8 Hz, 1H),2.53 (s, 3H), 2.17-2.08 (m, 1H), 2.04-1.62 (m, 9H), 1.52-1.27 (m, 12H),1.25-1.06 (m, 7H), 0.93 (t, J=7.2 Hz, 3H), 0.85-0.83 (d, J=6.8 Hz, 3H),0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₇O[M−H₂O+H]⁺455.4, found 455.4. SFC 100% de.

321: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.53-4.49 (m, 1H), 3.85-3.79 (m, 1H),3.55-3.53 (d, J=9.2 Hz, 1H), 3.33 (s, 3H), 3.21-3.19 (d, J=9.2 Hz, 1H),2.54 (s, 3H), 2.16-2.09 (m, 1H), 1.89-1.61 (m, 7H), 1.53-1.38 (m, 9H),1.36-1.09 (m, 12H), 0.94 (t, J=7.2 Hz, 3H), 0.80 (s, 3H), 0.71-0.69 (d,J=6.8 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₇O[M−H₂O+H]⁺455.4, found 455.4. SFC 100% de.

322: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.31-4.26 (m, 1H), 3.87-3.81 (m, 1H),3.56-3.54 (d, J=8.8 Hz, 1H), 3.33 (s, 3H), 3.19-3.17 (d, J=8.8 Hz, 1H),2.54 (s, 3H), 2.10-1.62 (m, 8H), 1.53-1.27 (m, 13H), 1.24-1.07 (m, 7H),0.94 (t, J=7.2 Hz, 3H), 0.82-0.80 (d, J=6.4 Hz, 3H), 0.70 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₇O [M−H₂O+H]+455.4, found455.4. SFC 100% de.

Examples 323 & 324:(3R,5R,8R,9R,10S,13S,14S,17R)-13-methyl-17-(1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(323)&(3R,5R,8R,9R,10S,13S,14S,17R)-13-methyl-17-(1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(324)

Synthesis of 323.2

To a solution of 323.1 (600 mg, 1.74 mmol) in THF (5 mL) was addedBH₃.Me₂S (0.87 mL, 8.7 mmol, 10 M) dropwise at 0° C. After stirring at25° C. for 3 h, the reaction mixture was cooled to 0° C. andsequentially treated with ethanol (800 mg, 17.4 mmol) at 0° C., NaOHaqueous (1.73 mL, 17.4 mmol, 5 M) dropwise and finally by H₂O₂ (1.73 mL,17.4 mmol) at 0° C. After stirring at 70° C. for 1 h, the mixture wasextracted with EtOAc (2×50 mL). The combined organic phase was washedwith saturated Na₂S₂O₃ aqueous (2×20 mL), brine (50 mL), dried overNa₂SO₄, filtered and evaporated to give 323.2 (620 mg) as an oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 3.85-3.58 (m, 1H), 3.52-3.29 (m, 1H),2.01-1.71 (m, 5H), 1.71-1.58 (m, 4H), 1.51-1.12 (m, 17H), 1.11-0.98 (m,6H), 0.97-0.90 (m, 5H), 0.68 (s, 3H).

Synthesis of 323.3 & 323.4

To a solution of 323.1 (24 g, 66.1 mmol) and5-methyl-2H-1,2,3,4-tetrazole (8.33 g, 99.1 mmol) in DMF (500 mL) wereadded Ph₃P (27.5 g, 105 mmol) and DEAD (18.2 g, 105 mmol). Afterstirring at 25° C. for 16 h, the mixture was poured into water (500 mL)and extracted with EtOAc (2×250 mL). The combined organic phase waswashed with brine (250 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 50% of EtOAcin PE) to give 323.3 (16.2 g, 57%) and 323.4 (10 g) both as solids.

Separation of 324 & 325

323.4 was triturated from MeOH/H₂O=1:1 (240 mL) at 25° C. and separatedby SFC (Column:DAICEL CHIRALCEL OD 250 mm×50 mm, 10 um; Condition: 0.1%NH₃H₂O ETOH; Gradient: from 40% to 40% of B; Flow rate: 200 mL/min;Column temperature: 40° C.) to afford 323 (1569.1 mg, 23%) and 324(2304.6 mg, 33.9%) both as solid.

323: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.33-4.24 (m, 1H), 3.91-3.80 (m, 1H),2.55 (s, 3H), 2.12-2.01 (m, 1H), 2.01-1.91 (m, 2H), 1.82-1.61 (m, 6H),1.56-1.51 (m, 2H), 1.49-1.42 (m, 3H), 1.40-1.29 (m, 8H), 1.27-1.01 (m,8H), 0.93 (t, J=7.2 Hz, 3H), 0.82 (d, J=6.4 Hz, 3H), 0.71 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₆H₄₃N₄[M−H₂O+H]⁺411.3 found411.3 analytic SFC 100% de.

324: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.54 (dd, J=4.4, 13.6 Hz, 1H), 3.84(dd, J=11.2, 13.6 Hz, 1H), 2.55 (s, 3H), 2.20-2.04 (m, 1H), 1.91-1.62(m, 8H), 1.55-1.44 (m, 4H), 1.41-1.24 (m, 12H), 1.16_1.04 (m, 5H), 0.93(t, J=7.2 Hz, 3H), 0.82 (s, 3H), 0.70 (d, J=6.4 Hz, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₆H₄₃N₄[M−H₂O+H]⁺411.3 found 411.3analytic SFC 100% de.

Examples 325 & 326:1-((S)-2-((3R,5R,8S,9S,10S,13S,14S,17R)-10-ethyl-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(325) &1-((R)-2-((3R,5R,8S,9S,10S,13S,14S,17R)-10-ethyl-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(326)

Synthesis of 325.1

To a solution of BHT (26 g, 118 mmol) in toluene (60 mL) was added AlMe₃(2 M in toluene, 29.7 mL, 59.4 mmol) dropwise at 0° C. under N₂. Afterstirring at 15° C. for 1 h, to the MAD (59.4 mmol in 60 mL toluene)solution was added a solution of 301.4 (6.0 g, 19.8 mmol) in DCM (10 mL)dropwise at −70° C. After stirring at −70° C. for 1 h under N₂, MeMgBr(19.8 mL, 59.4 mmol, 3M in ethyl ether) was added dropwise at −70° C.After stirring at −70° C. for another 4 h, the reaction mixture waspoured into saturated 20% citric acid (300 mL) at 10° C. and extractedwith EtOAc (2×100 mL). The combined organic layer was dried over Na₂SO₄,filtered and concentrated in vacuum. The residue was purified by flashcolumn (0˜20% of EtOAc in PE) to give 325.1 (5.6 g, 88.8%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.50-2.37 (m, 1H), 2.13-2.04 (m, 1H),2.04-1.81 (m, 3H), 1.81-1.62 (m, 5H), 1.62-1.47 (m, 5H), 1.46-1.28 (m,6H), 1.25 (s, 3H), 1.24-1.11 (m, 4H), 0.84 (s, 3H), 0.80 (t, J=7.60 Hz,3H).

Synthesis of 325.2

To a mixture of EtPPh₃Br (9.72 g, 26.2 mmol) in THF (50 mL) was addedt-BuOK (2.93 g, 26.2 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 mins, 325.1 (5.6 g, 17.5 mmol) was added in portions below 40° C.After stirring at 40° C. for 1 h, the reaction mixture was quenched with10% NH₄Cl aqueous (200 mL) at 15° C. and extracted with EtOAc (300 mL).The combined organic phase was concentrated under vacuum to give asolid, which was purified by flash column (5%-20% of EtOAc in PE) toafford 325.2 (4.9 g, 84.7%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-5.05 (m, 1H), 2.41-2.09 (m, 3H),2.03-1.89 (m, 1H), 1.85-1.71 (m, 1H), 1.70-1.59 (m, 6H), 1.59-1.37 (m,9H), 1.37-1.27 (m, 3H), 1.25 (s, 3H), 1.22-1.04 (m, 5H), 0.85 (s, 3H),0.79 (t, J=7.6 Hz, 3H).

Synthesis of 325.3

To a solution of 325.2 (4.9 g, 14.8 mmol) in THF (50 mL) was added 9-BBNdimer (10.8 g, 44.4 mmol) at 15° C. After stirring at 40° C. for 1 h,the reaction was sequentially treated with ethanol (6.8 g, 148 mmol) at15° C., NaOH aqueous (29.5 mL, 5M, 148 mmol) at −10° C. and finally byH₂O₂ (14.7 mL, 10 M, 148 mmol) dropwise. After stirring at 80° C. for 1h, the reaction was quenched with aqueous sat. Na₂S₂O₃ (50 mL), stirredfor 30 mins and extracted with EtOAc (100 mL). The combined organicphase was washed with brine (2×100 mL), dried over anhydrous Na₂SO₄,concentrated under vacuum to give 325.3 (11 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.62 (m, 1H), 2.03-1.91 (m, 3H),1.83-1.71 (m, 5H), 1.57-1.45 (m, 12H), 1.24 (s, 3H), 1.21 (d, J=6.40 Hz,3H), 1.18-0.94 (m, 7H), 0.81-0.76 (m, 3H), 0.64 (s, 3H).

Synthesis of 325.4

To a solution of 325.3 (5.15 g, 14.8 mmol) in DCM (100 mL) was addedsilica gel (10 g) and PCC (6.36 g, 29.6 mmol) at 0° C. After stirring at15° C. for 3 h, the suspension was filtered and the filter cake waswashed with DCM (2×100 mL). The combined filtrate was concentrated undervacuum to give a solid, which was purified by flash column(PE/EtOAc=20/1 to 4/1) to afford 325.4 (2.8 g, 54.6%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.60-2.45 (m, 1H) 2.28-2.12 (m, 1H), 2.11(s, 3H), 2.03-1.91 (m, 2H), 1.82-1.59 (m, 6H), 1.54-1.28 (m, 10H), 1.25(s, 3H), 1.24-1.03 (m, 6H), 0.79 (t, J=7.60 Hz, 3H), 0.59 (s, 3H).

Synthesis of 325.5

To a mixture of MePPh₃Br (4.5 g, 12.6 mmol) in THF (20 mL) was addedt-BuOK (1.41 g, 12.6 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 mins, 325.4 (2.2 g, 6.34 mmol) was added in portions below 50° C.After stirring at 50° C. for 1 h, the reaction mixture was quenched with10% NH₄Cl aqueous (100 mL) at 15° C. and extracted with EtOAc (100 mL).The combined organic phase was concentrated under vacuum to give asolid. The residue was purified by silica gel chromatography(PE/EtOAc=20/1 to 5/1) to afford 325.5 (1.6 g, 73.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.69 (s, 1H), 2.04-1.90 (m,2H), 1.87-1.76 (m, 2H), 1.75 (s, 3H), 1.73-1.57 (m, 5H), 1.53-1.26 (m,9H), 1.25 (s, 3H), 1.23-0.82 (m, 8H), 0.79 (t, J=7.60 Hz, 3H), 0.54 (s,3H).

Synthesis of 325.6

To a solution of 325.5 (400 mg, 1.16 mmol) in THF (10 mL) was addedBH₃Me₂S (0.348 mL, 10 M in DMS, 3.48 mmol) at 15° C. After stirring at15° C. for 16 h, the reaction was treated sequentially with EtOH (533mg, 11.6 mmol) at 15° C., NaOH (2.32 mL, 5M in water, 11.6 mmol) at 0°C. and finally by H₂O₂ (1.2 mL, 10 M, 11.6 mmol) dropwise. Afterstirring at 80° C. for 1 h, the reaction mixture was quenched with sat.Na₂S₂O₃ (50 mL), stirred for 30 mins. and extracted with EtOAc (100 mL).The combined organic phase was washed with brine (2×100 mL), dried overanhydrous Na₂SO₄ and concentrated under vacuum to give 325.6 (400 mg,95.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.70 (m, 1H), 3.48-3.40 (m, 1H),2.01-1.71 (m, 6H), 1.70-1.58 (m, 4H), 1.50-1.37 (m, 5H), 1.34-1.28 (m,2H), 1.24 (s, 3H), 1.22-0.98 (m, 11H), 0.95 (d, J=6.80 Hz, 3H), 0.79 (t,J=7.60 Hz, 3H), 0.66 (s, 3H).

Synthesis of 325.7

To a solution of 325.6 (400 mg, 1.1 mmol) in DCM (10 mL) was added PPh₃(432 mg, 1.65 mmol) and NBS (293 mg, 1.65 mmol) at 0° C. After stirringat 20° C. for 2 h, the mixture was added into water (50 mL) andextracted with DCM (2×50 mL). The combined organic phase was washed withbrine (100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated.The residue was purified by flash column (0˜15% of EtOAc in PE) to give325.7 (400 mg, 85.4%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.66-3.47 (m, 1H), 3.41-3.28 (m, 1H),2.03-1.71 (m, 5H), 1.71-1.59 (m, 3H), 1.56-1.49 (m, 2H), 1.48-1.28 (m,12H), 1.24 (s, 3H), 1.23-1.10 (m, 5H), 1.07 (d, J=6.40 Hz, 2H), 1.00 (d,J=6.40 Hz, 2H), 0.79 (t, J=7.60 Hz, 3H), 0.67-0.65 (m, 3H).

Synthesis of 325.8

To a solution of 325.7 (200 mg, 0.47 mmol) in DMF (10 mL) were addedCs₂CO₃ (306 mg, 0.94 mmol) and 1H-pyrazole-4-carbonitrile (65.6 mg,0.705 mmol). After stirring at 80° C. for 3 h under N₂, the mixture wasadded into saturated NH₄Cl (100 mL) and extracted with EtOAc (3×100 mL).The combined organic layer was washed with LiCl (100 mL, 5% in water),brine (2×100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to afford 325.8 (200 mg, 97.5%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.76 (s, 1H), 4.57-4.19 (m,1H), 3.78-3.52 (m, 1H), 2.19-2.05 (m, 1H), 2.04-1.71 (m, 6H), 1.57-1.32(m, 11H), 1.25 (s, 3H), 1.22-1.01 (m, 9H), 0.79 (s, 3H), 0.77 (s, 3H),0.64-0.70 (m, 3H).

Synthesis of 325 & 326

325.8 (200 mg, 0.46 mmol) was separated by SFC (Column: DAICEL CHIRALPAKAD (250 mm*50 mm, 10 um), Condition: 0.1% NH₃H₂O IPA, Begin B: 30%, EndB: 30%, FlowRate (ml/min): 70) to afford 325 (60 mg, 30.1%) as a solidand 326 (65 mg, 32.6%) as a solid.

325: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.48 (dd,J=13.40, 4.40 Hz, 1H), 3.65 (dd, J=13.20, 10.80 Hz, 1H), 2.16-2.02 (m,1H), 2.00-1.56 (m, 8H), 1.54-1.43 (m, 2H), 1.43-1.27 (m, 8H), 1.25 (s,3H), 1.24-1.02 (m, 8H), 0.84-0.74 (m, 6H), 0.67 (d, J=6.60 Hz, 3H).LC-ELSD/MS: purity >99%; analytic SFC: 99.28% de; MS ESI calcd. forC₂₈H₄₂N₃[M−H₂O+H]⁺420.3, found 420.3. SFC 100% de.

326: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.25 (dd,J=13.40, 3.60 Hz, 1H), 3.72 (dd, J=13.40, 9.60 Hz, 1H), 2.10-1.86 (m,4H), 1.84-1.59 (m, 4H), 1.55-1.49 (m, 2H), 1.49-1.26 (m, 9H), 1.25 (s,3H), 1.22-1.04 (m, 8H), 0.82-0.75 (m, 6H), 0.69 (s, 3H). LC-ELSD/MS:purity >99%; analytic SFC:100% de; MS ESI calcd. forC₂₈H₄₂N₃[M−H₂O+H]⁺420.3, found 420.3. SFC 100% de.

Examples 327 & 328:1-((R)-2-((3R,5R,8S,9S,10S,11S,13S,14S,17R)-3,11-dihydroxy-3-(methoxymethyl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(327) &1-((S)-2-((3R,5R,8S,9S,10S,11S,13S,14S,17R)-3,11-dihydroxy-3-(methoxymethyl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(328)

Synthesis of 327.1

To a solution of Me₃SI (2.75 g, 13.5 mmol) in THF (30 mL) and DMSO (30mL) was added NaH (540 mg, 13.5 mmol, 60%) at 0° C. in portions underN₂. After stirring at 0° C. for 1 h, to the resulting mixture was addeda solution of 309.0 (3 g, 9.02 mmol) in DMSO (30 mL). After stirring at25° C. for another 2 h, the resulting suspension was poured into water(200 mL), the aqueous phase was extracted with EtOAc (2×300 mL). Thecombined organic phase was washed with brine (200 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 15% of EtOAc in PE) to give 327.1 (1.4 g) as oil.

Synthesis of 327.2

To a solution of 327.1 (820 mg, 2.36 mmol) in MeOH (15 mL) was addedMeONa (1.27 g, 23.6 mmol). After stirring at 50° C. for 16 h, thereaction mixture was combined with another batch prepared from 1.4 g of327.1, poured into water (100 mL) and extracted with ethyl acetate(2×150 mL). The combined organic layers were washed with saturatedaqueous NH₄Cl (100 mL), brine (100 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue, which was combined with 530 mgof 327.2, and purified by flash column (0˜ 40% ethyl acetate in PE) togive 327.2 (870 mg) as oil.

Synthesis of 327.3

To a suspension of MePh₃PBr (6.68 g, 18.7 mmol) in anhydrous THF (50 mL)was added t-BuOK (2.09 g, 18.7 mmol) at 25° C. under N₂. After stirringat 60° C. for 30 mins, a solution of 327.2 (710 mg, 1.87 mmol) inanhydrous THF (10 mL) was added dropwise. After stirring at 60° C. for16 h, the mixture was cooled and poured into ice-water (100 mL), stirredfor 10 mins. and extracted with EtOAc (2×150 mL). The combined organicphase was washed with brine (2×100 mL), filtered and concentrated. Theresidue was purified by flash column (0˜ 25% of EtOAc in PE) to give327.3 (290 mg) as a solid.

Synthesis of 327.4

To a solution of 327.3 (290 mg, 0.770 mmol) in THF (15 mL) was addedBH₃Me₂S (0.384 mL, 10 M, 3.84 mmol) at 25° C. After stirring at 25° C.for 16 h, the reaction was sequentially treated with EtOH (1.33 mL, 23.0mmol) at 25° C., NaOH (4.60 mL, 5.0 M, 23.0 mmol) at 0° C., and H₂O₂(2.30 mL, 23.0 mmol, 30% in water) dropwise. After stirring at 70° C.for 1 h, the mixture was poured into water (80 mL) and extracted withEtOAc (2×100 mL). The combined organic layer was washed with saturatedNa₂S₂O₃ (100 mL), brine (100 mL), dried over anhydrous Na₂SO₄, filteredand concentrated to give 327.4 (320 mg) as oil.

Synthesis of 327.5

To a solution of 327.4 (320 mg, 0.810 mmol) in DMF (10 mL) were addedPh₃P (1.06 g, 4.05 mmol), DEAD (705 mg, 0.637 mL, 4.05 mmol) and1H-pyrazole-4-carbonitrile (149 mg, 1.61 mmol) at 0° C. After stirringat 25° C. for 16 h, the mixture was poured into water (30 mL) andextracted with EtOAc (2×50 mL). The combined organic phase was washedwith brine (30 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated.

Separation of 327 & 328

325.5 was separated by pre-HPLC (Column: Welch Xtimate C18 150*25 mm*5um; Condition: water (0.05% NH₃H₂O)-ACN; Begin B:55%; End B:85%) toafford 327 (20 mg) and 450 (60 mg) both as solids. 327 (60 mg) wasrepurified by SFC (Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, 5 um);Condition: 0.1% NH₃H₂O ETOH; Begin B: 45%; End B: 45%; Flow Rate(ml/min): 50) to give 327 (Peak 3, Rt=4.125 min, 7 mg, 11.7%) as asolid. 328 (95 mg) was repurified by SFC (Column: DAICEL CHIRALCEL OD(250 mm*30 mm, 10 um); Condition: 0.1% NH₃H₂O ETOH; Begin B: 60%; End B:60%; Flow Rate (ml/min):80) to give 328 (Peak 2, Rt=6.567 min, 27 mg,28.4%) as a solid.

327: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.76 (s, 1H), 4.53-4.47(m, 1H), 4.19 (br s, 1H), 3.70-3.62 (m, 1H), 3.44-3.36 (m, 5H), 2.69 (s,1H), 2.16-2.01 (m, 2H), 1.94-1.80 (m, 4H), 1.79-1.65 (m, 3H), 1.50-1.22(m, 7H), 1.18 (s, 4H), 1.17-1.07 (m, 4H), 1.06-1.03 (m, 1H), 1.00 (s,3H), 0.67 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₈H₄₀N₃O [M-2H₂O+H]⁺434.3 found 434.3. SFC 100% de.

328: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.27-4.16(m, 2H), 3.77-3.68 (m, 1H), 3.40 (s, 5H), 2.04-1.96 (m, 1H), 1.94-1.79(m, 4H), 1.78-1.63 (m, 3H), 1.56-1.53 (m, 1H), 1.48-1.38 (m, 4H),1.30-1.22 (m, 2H), 1.18 (s, 4H), 1.13-1.06 (m, 3H), 1.02-0.97 (m, 1H),0.92 (s, 3H), 0.82 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₀N₃O [M-2H₂O+H]⁺434.3 found 434.3. SFC 100% de.

Example 329-332: Synthesis of(3R,5S,8R,9R,10S,13S,14S,17R)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(329) &(3R,5S,8R,9R,10S,13S,14S,17R)-13-methyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(330) &(3R,5S,8R,9R,10S,13S,14S,17R)-13-methyl-17-((S)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(331) &(3R,5S,8R,9R,10S,13S,14S,17R)-13-methyl-17-((R)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(332)

Synthesis of 329.1

Lithium (7.27 g, 915 mmol) was added to fresh prepared liquid ammonia(500 mL) in portions at −70° C. under N₂. After stirring at −70° C. for1 h, a solution of 329.0 (50 g, 183 mmol) and t-butanol (27 g, 366 mmol)in THF (500 mL) was added below −60° C. After stirring at −70° C. for 1h, ammonium chloride (500 g) was added, then the mixture was warmed to25° C. After stirring for 16 h, the reaction mixture was added H₂O (1 L)and extracted with EtOAc (3×500 mL). The combined organic solution waswashed with 1 M HCl (2×500 mL), saturated NaHCO₃ aqueous (500 mL), brine(1 L), dried over Na₂SO₄ and concentrated under vacuum to give 329.1 (97g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.75-3.55 (m, 1H), 2.50-2.00 (m, 10H),2.00-1.25 (m, 8H), 1.25-0.60 (m, 9H).

Synthesis of 329.2

To a solution of 329.1 (100 g, 361 mmol) in DCM (1000 mL) were addedsilica gel (116 g) and PCC (116 g, 541 mmol) at 0° C. After stirring at25° C. for 2 h, PE (1000 mL) was added to the reaction mixture. Theresulting mixture was filtered through a pad of silica gel and thefilter cake was washed with DCM (2000 mL). The filtrate was concentratedto give 329.2 (90 g) as oil. ¹H NMR (400 MHz, CDCl₃) δ_(H) 2.55-2.02 (m,8H), 2.02-1.39 (m, 8H), 1.39-0.69 (m, 10H).

Synthesis of 329.3

To a solution of 329.2 (15 g, 54.6 mmol) in THF (200 mL) was addedn-PrMgCl (81.5 mL, 163 mmol, 2M in THF) dropwise at −60° C. under N₂.After stirring at −60° C. for 2 h, the reaction mixture was poured intosaturated aqueous NH₄Cl (100 mL) at 0° C. and extracted with EtOAc(2×200 mL). The combined organic layer was dried over Na₂SO₄, filteredand concentrated. The residue was triturated from MeCN (50 mL) at 80° C.to give 329.3 (7 g, 40.4%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.44 (dd, J=8.4, 19.2 Hz, 1H), 2.14-2.00(m, 1H), 1.99-1.84 (m, 2H), 1.83-1.71 (m, 3H), 1.70-1.44 (m, 5H),1.43-1.12 (m, 10H), 1.11-0.99 (m, 4H), 0.97-0.90 (m, 3H), 0.88 (s, 3H),0.81-0.66 (m, 2H).

Synthesis of 329.4

To a mixture of EtPPh₃Br (24.3 g, 65.6 mmol) in THF (80 mL) was addedt-BuOK (7.36 g, 65.6 mmol) at 15° C. under N₂. After stirring for 30mins, 329.3 (7 g, 21.9 mmol) in THF (20 mL) was added. After stirring at40° C. for 1 h, the mixture was poured into NH₄Cl (50 mL) and extractedwith EtOAc (2×100 mL). The combined organic phase was washed with brine(100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was heated at 70° C. in MeOH (50 mL) for 30 minutes, cooled toroom temperature, poured into water (50 mL), filtered to give 329.4 (11g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.24-4.98 (m, 1H), 2.45-2.30 (m, 1H),2.28-2.11 (m, 2H), 1.88-1.74 (m, 2H), 1.73-1.57 (m, 7H), 1.55-1.48 (m,2H), 1.44-1.25 (m, 6H), 1.24-0.96 (m, 9H), 0.95-0.90 (m, 3H), 0.88 (s,3H), 0.78-0.62 (m, 2H).

Synthesis of 329.5

To a solution of 329.4 (6 g, 18.1 mmol) in anhydrous THF (60 mL) wasadded 9-BBN dimer (13.2 g, 54.3 mmol) at 15° C. under N₂. After stirringat 60° C. for 2 h, the mixture was cooled and treated sequentially withEtOH (15 mL), NaOH (15 mL, 5M, 75.5 mmol) very slowly and H₂O₂ (22.6 mL,226 mmol, 10 M) slowly below 30° C. After stirring at 60° C. for 2 h,the mixture was cooled, poured into water (50 mL) and extracted withEtOAc (2×50 mL). The combined organic layer was dried over Na₂SO₄,filtered and concentrated in vacuum. The product was purified by flashcolumn (20-25% of EtOAc in PE) to give 329.5 (6.1 g, 52.8%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.86-3.57 (m, 1H), 1.98-1.81 (m, 2H),1.81-1.70 (m, 2H), 1.70-1.60 (m, 3H), 1.57-1.48 (m, 3H), 1.42-1.25 (m,7H), 1.23 (d, J=6.0 Hz, 3H), 1.17-0.96 (m, 9H), 0.95-0.89 (m, 3H), 0.67(s, 5H).

Synthesis of 329.6

To a solution of 329.5 (6.1 g, 17.5 mmol) in DCM (50 mL) was added PCC(11.2 g, 52.5 mmol) and silica gel (15 g) at 25° C. After stirring at25° C. for 1 h, the reaction mixture was filtered and the residue waswashed with DCM (2×20 mL). The combined filtrate was concentrated invacuum. The residue was purified by flash column (15-20% of EtOAc in PE)to give 329.6 (3 g, 49.5%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.55 (t, J=8.8 Hz, 1H), 2.12 (s, 4H), 2.00(td, J=3.2, 12.0 Hz, 1H), 1.89-1.73 (m, 2H), 1.59 (br d, J=2.8 Hz, 1H),1.55-1.50 (m, 1H), 1.49-1.16 (m, 10H), 1.15-0.97 (m, 6H), 0.96-0.90 (m,3H), 0.81-0.65 (m, 2H), 0.62 (s, 3H).

Synthesis of 329.7

To a mixture of MePPh₃Br (9.25 g, 25.9 mmol) in THF (40 mL) was addedt-BuOK (2.9 g, 25.9 mmol) at 15° C. under N₂. After stirring 30 mins,329.6 (3 g, 8.65 mmol) in THF (10 mL) was added. After stirring at 40°C. for 2 h, the mixture was poured into NH₄Cl.aq (150 mL) and extractedwith EtOAc (2×200 mL). The combined organic phase was washed with brine(20 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was heated in MeOH (500 mL) at 70° C. for 30 minutes, cooled toroom temperature, added water (300 mL), filtered and concentrated togive 329.7 (3 g, 100%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.85 (s, 1H), 4.71 (s, 1H), 2.11-1.98 (m,1H), 1.76 (s, 7H), 1.71-1.64 (m, 3H), 1.64-1.53 (m, 3H), 1.39 (d, J=3.6Hz, 4H), 1.14 (br s, 12H), 0.95-0.88 (m, 3H), 0.77-0.62 (m, 2H), 0.58(s, 3H).

Synthesis of 329.8

To a solution of 329.7 (2.6 g, 7.54 mmol) in THF (30 mL) was added BH₃.Me₂S (2.26 mL, 22.6 mmol, 10M) at 0° C. under N₂. After stirring at 20°C. for 3 h, the reaction mixture was treated sequentially with ethanol(6.50 mL, 113 mmol) at 0° C., NaOH aqueous (22.6 mL, 5.0 M, 113 mmol),and then hydrogen peroxide (11.3 mL, 10 M, 113 mmol) dropwise. After 1hr, the reaction was quenched with Na₂S₂O₃ (30 mL), stirred for 10minutes and extracted with EtOAc (2×50 mL). The combined organic phasewas washed with brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜25% of EtOAcin PE) to give 329.8 (3.1 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.79-3.58 (m, 1H), 3.54-3.29 (m, 1H),1.88-1.58 (m, 10H), 1.56-1.47 (m, 2H), 1.38 (d, J=3.6 Hz, 4H), 1.34-1.23(m, 3H), 1.22-1.15 (m, 2H), 1.14-0.97 (m, 9H), 0.95 (d, J=6.8 Hz, 2H),0.93-0.89 (m, 3H), 0.68 (s, 5H).

Synthesis of 329.9

To a solution of 329.8 (500 mg, 1.37 mmol) in DCM (5 mL) were added PPh₃(574 mg, 2.19 mmol) and NBS (387 mg, 2.19 mmol) at 0° C. under N₂, Afterstirring at 25° C. for 3 h, the mixture was concentrated and purified byflash column (0˜3% of EtOAc in PE) to give 329.9 (560 mg, 96.2%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.68-3.47 (m, 1H), 3.41-3.30 (m, 1H),1.96-1.70 (m, 5H), 1.67-1.47 (m, 7H), 1.38 (d, J=3.2 Hz, 4H), 1.35-1.22(m, 6H), 1.16-0.99 (m, 6H), 0.99-0.86 (m, 6H), 0.68 (s, 5H).

Synthesis of 329.10 and 331.1

To a solution of 329.9 (560 mg, 1.31 mmol) in DMF (10 mL) were added5-methyl-2H-1,2,3,4-tetrazole (164 mg, 1.96 mmol) and Cs₂CO₃ (2.13 g,6.55 mmol) at 20° C. under N₂. After stirring at 120° C. for 2 h, thereaction mixture was diluted with water (100 mL) and extracted withethyl acetate (3×150 mL). The combined organic layers were washed withbrine (150 mL), dried over anhydrous Na₂SO₄, filtered and concentratedunder vacuum to dryness. The residue was purified by flash column(0˜100% ethyl acetate in PE) to give 329.10 (390 mg, 69.5%) and 331.1(170 mg, 30.3%) both as solids.

Separation of 329.10

329.10 (390 mg, 0.909 mmol) was separated by SFC (Column: DAICELCHIRALPAK AD (250 mm*30 mm, 10 um); Condition: 0.1% NH₃H₂O ETOH; Begin:B 60%; End B: 60%; Flow Rate(ml/min): 80) to give 329 (Peak 2, Rt=1.828min, 207.5 mg, 53.2%) as a solid and 3330 (Peak 1, Rt=1.680 min, 95.6mg, 24.5%) as a solid. The stereochemistry at C20 was assigned based on¹H NMR of C21-Me (C18-Me with C21-down-Et is at more downfield thanC21-up isomer).

329: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.77 (dd, J=13.2, 4.4 Hz, 1H), 4.24(dd, J=13.2, 10.0 Hz, 1H), 2.54 (s, 3H), 2.31-2.15 (m, 1H), 1.93-1.82(m, 2H), 1.82-1.72 (m, 2H), 1.65 (ddd, J=9.2, 6.4, 2.4 Hz, 2H), 1.59 (s,1H), 1.58-1.48 (m, 2H), 1.39 (d, J=3.2 Hz, 3H), 1.37-1.32 (m, 2H),1.31-1.18 (m, 3H), 1.18-1.09 (m, 5H), 1.08-1.02 (m, 2H), 0.99 (br dd,J=12.4, 5.2 Hz, 2H), 0.92 (br s, 3H), 0.82 (s, 3H), 0.71 (d, J=6.4 Hz,4H), 0.66 (br d, J=3.2 Hz, 1H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₆H₄₄N₄O [M+H]⁺ 429.3, found 429.3. SFC 100% de.

330: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.53 (dd, J=13.2, 3.6 Hz, 1H), 4.29(dd, J=13.2, 9.2 Hz, 1H), 2.54 (s, 3H), 2.21-2.08 (m, 1H), 2.05-1.89 (m,2H), 1.74 (br dd, J=12.8, 2.8 Hz, 2H), 1.70-1.57 (m, 4H), 1.53-1.57 (m,1H), 1.52-1.40 (m, 2H), 1.39 (d, J=3.2 Hz, 3H), 1.35-1.24 (m, 2H),1.23-1.16 (m, 2H), 1.13 (s, 2H), 1.12-1.00 (m, 5H), 0.99-0.95 (m, 1H),0.92 (br s, 3H), 0.86 (d, J=6.8 Hz, 3H), 0.73 (s, 3H), 0.71-0.63 (m,2H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₆H₄₄N₄O [M+H]⁺ 429.3,found 429.4. SFC 100% de.

Separation of 331 & 332

331.1 (170 mg, 0.396 mmol) was separated by SFC (Column: DAICELCHIRALCEL OJ-H (250 mm*30 mm, 5 um); Condition: 0.1% NH₃H₂O ETOH; Begin:B 20%; End B: 20%; Flow Rate(ml/min): 60) to give 331 (Peak 1, Rt=2.220min, 30.9 mg, 18.2%) as a solid and 332 (Peak 2, Rt=2.354 min, 62.9 mg,37.2%) as a solid. The stereochemistry at C20 was assigned based on ¹HNMR of C21-Me (C18-Me with C21-down-Et is at more downfield than C21-upisomer).

331: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.29 (dd, J=13.6, 3.6 Hz, 1H), 3.87(dd, J=13.6, 10.4 Hz, 1H), 2.55 (s, 3H), 2.11-1.91 (m, 3H), 1.75 (br d,J=14.4 Hz, 2H), 1.71-1.61 (m, 3H), 1.60-1.50 (m, 3H), 1.49-1.42 (m, 1H),1.39 (br d, J=3.2 Hz, 4H), 1.34-1.22 (m, 3H), 1.20-1.14 (m, 2H), 1.12(s, 2H), 1.10-0.97 (m, 5H), 0.92 (br s, 3H), 0.84 (d, J=6.4 Hz, 3H),0.74-0.62 (m, 5H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₆H₄₄N₄O[M+H]⁺ 429.3, found 429.4. SFC 100% de.

332: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.55 (dd, J=13.6, 4.4 Hz, 1H), 3.85(dd, J=13.6, 11.2 Hz, 1H), 2.56 (s, 3H), 2.20-2.07 (m, 1H), 1.94-1.83(m, 2H), 1.82-1.72 (m, 2H), 1.69-1.62 (m, 3H), 1.61-1.50 (m, 3H), 1.39(br d, J=3.6 Hz, 4H), 1.35 (br dd, J=10.0, 3.2 Hz, 2H), 1.32-1.27 (m,2H), 1.26-1.21 (m, 1H), 1.20-1.13 (m, 2H), 1.12 (s, 2H), 1.10-0.96 (m,4H), 0.93 (br s, 3H), 0.83 (s, 3H), 0.76-0.64 (m, 5H). LC-ELSD/MS purity99%, MS ESI calcd. for C₂₆H₄₄N₄O [M+H]⁺ 429.3, found 429.4. SFC 100% de.

Examples 333-336: Synthesis of(3R,5S,8R,9R,10S,13S,14S,17R)-3-ethyl-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(333) &(3R,5S,8R,9R,10S,13S,14S,17R)-3-ethyl-13-methyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(334) &(3R,5S,8R,9R,10S,13S,14S,17R)-3-ethyl-13-methyl-17-((R)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(335) &(3R,5S,8R,9R,10S,13S,14S,17R)-3-ethyl-13-methyl-17-((S)-1-(5-methyl-1H-tetrazol-1-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(336)

Synthesis of 333.2

To a solution of 333.1 (51.5 g, 187 mmol) in MeOH (600 mL) was added4-methylbenzenesulfonic acid (6.44 g, 37.4 mmol) at 25° C. under N₂.After stirring at 55° C. for 16 h, Et₃N (20 mL) was added, and themixture was filtered to afford 333.2 (57 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.49 (d, J=5.6 Hz, 1H), 3.20 (s, 3H), 3.14(s, 3H), 2.48-2.38 (m, 1H), 2.12-2.01 (m, 2H), 1.96-1.90 (m, 2H),1.88-1.74 (m, 4H), 1.68-1.62 (m, 1H), 1.56-1.44 (m, 1H), 1.35-1.20 (m,5H), 1.13-0.95 (m, 5H), 0.87 (s, 1H), 0.80-0.68 (m, 2H).

Synthesis of 333.3

To a mixture of EtPPh₃Br (98.7 g, 266 mmol) in THF (250 mL) was addedt-BuOK (29.8 g, 266 mmol) at 15° C. under N₂. After stirred at 15° C.for 30 mins, 333.2 (28.5 g, 88.9 mmol) in THF (50 mL) was added. Afterstirring at 40° C. for 2 h, the mixture was poured into NH₄Cl.aq (150mL) and extracted with EtOAc (2×200 mL). The combined organic phase waswashed with brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was heated in MeOH (500 mL) at 70° C. for 30minutes, cooled to room temperature, diluted with water (300 mL),filtered and concentrated to give 333.3 (25.5 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ 5.15-5.07 (m, 1H), 3.20 (s, 3H), 3.14 (s, 3H),2.41-2.31 (m, 1H), 2.27-2.12 (m, 2H), 2.10-2.02 (m, 1H), 1.91 (td,J=3.2, 13.2 Hz, 1H), 1.85-1.76 (m, 2H), 1.71-1.58 (m, 6H), 1.57-1.48 (m,3H), 1.30-1.13 (m, 6H), 1.11-0.93 (m, 5H), 0.87 (s, 3H), 0.75-0.67 (m,2H).

Synthesis of 333.4

To a solution of 333.3 (51 g, 153 mmol) in THF (500 mL) was added HCl(153 mL, 1 M, 153 mmol) at 15° C. After stirring for 2 h, the mixturewas poured into aq NaHCO₃ (400 mL) and extracted with EtOAc (2×300 mL).The combined organic phase was washed with brine (200 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 333.4 (42 g, 95.8%)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.16-5.08 (m, 1H), 2.42-2.17 (m, 7H), 2.09(t, J=13.2 Hz, 1H), 1.88-1.79 (m, 2H), 1.76-1.63 (m, 6H), 1.59 (s, 1H),1.56-1.40 (m, 2H), 1.28-1.16 (m, 8H), 1.04-0.94 (m, 1H), 0.90 (s, 1H),0.78-0.69 (m, 1H).

Synthesis of 333.5

To solution of Me₃SIO (47.9 g, 218 mmol) in DMSO (300 mL) and THF (300mL) was added NaH (5.23 g, 218 mmol) at 0° C. under N₂. After stirringfor 1 h, 333.4 (42 g, 146 mmol) in THF (200 mL) was added. Afterstirring at 25° C. for 3 h, the reaction mixture was poured into water(1000 mL), stirred at 25° C. for 3 h and filtered to give 333.5 (48 g)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-5.07 (m, 1H), 2.64-2.61 (m, 5H),2.41-2.30 (m, 1H), 2.27-2.11 (m, 2H), 2.00-1.92 (m, 1H), 1.91-1.80 (m,2H), 1.67-1.60 (m, 5H), 1.56-1.50 (m, 1H), 1.45-1.35 (m, 1H), 1.30-1.10(m, 8H), 1.07-0.95 (m, 2H), 0.89 (s, 3H), 0.84-0.72 (m, 2H).

Synthesis of 333.6

To a suspension of CuCN (3.92 g, 43.8 mmol) in THF (40 mL) was addedMeLi (54.7 mL, 87.6 mmol, 1.6M) at −70° C. under N₂. After stirring at−70° C. for 1 h, 333.6 (4.4 g, 14.6 mmol) in THF (10 mL) was added at−70° C. After stirring at 25° C. for 2 h, the reaction was slowly pouredinto 10% NH₄Cl (20 mL) and extracted with EtOAc (2×50 mL). The combinedorganic phase was washed with brine (20 mL), dried over anhydrousNa₂SO₄, filtered and concentrated to give 333.6 (4.4 g) as a solid.

1H NMR (400 MHz, CDCl₃) δ 5.14-5.08 (m, 1H), 2.39-2.10 (m, 3H),1.85-1.39 (m, 10H), 1.39-0.94 (m, 13H), 0.94-0.60 (m, 9H).

Synthesis of 333.7

To a solution of 333.6 (4.4 g, 13.3 mmol) in anhydrous THF (50 mL) wasadded 9-BBN dimer (8.03 g, 33.2 mmol) at 25° C. under N₂. After stirringat 60° C. for 16 h, the mixture was cooled and sequentially treated withEtOH (20 mL) at 0° C., NaOH (2.66 g, 13.3 mL, 5M, 66.5 mmol) very slowlyand H₂O₂ (13.3 mL, 133 mmol, 10 M in water) slowly until the reactiontemperature no longer rises and the reaction temperature was maintainedbelow 30° C. After stirring at 60° C. for 2 h, the mixture was cooled,quenched with Na₂S₂O₃ (100 mL, sat. aq.) and extracted with EtOAc (3×100mL). The combined organic layer was dried over Na₂SO₄, filtered andconcentrated in vacuum. The residue was purified by column (5%-30% ofEtOAc in PE) to give 333.7 (10 g) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H)3.74-3.66 (m, 1H), 1.96-1.39 (m, 13H),1.39-1.00 (m, 14H), 1.00-0.85 (m, 5H), 0.75-0.57 (m, 5H).

Synthesis of 333.8

To a solution of 333.7 (1.3 g, 3.88 mmol) in DCM (20 mL) was added DMP(3.29 g, 7.76 mmol). After stirring at 25° C. for 1 h, the mixture wasquenched with NaHCO₃ (50 mL) and extracted with EtOAc (3×30 mL). Theorganic layer was washed with Na₂S₂O₃ (3×30 mL, sat.), brine (50 mL),dried over Na₂SO₄, filtered and concentrated in vacuum. The residue waspurified by column (5%-30% of EtOAc in PE) to give to give 333.8 (1.16g, 90%) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H)2.53 (t, J=8.8 Hz, 1H), 2.21-1.53 (m, 9H),1.53-1.10 (m, 10H), 1.10-0.63 (m, 13H), 0.61 (s, 3H).

Synthesis of 333.9

To a mixture of MePPh₃Br (2.48 g, 6.96 mmol) in THF (40 mL) was addedt-BuOK (779 mg, 6.96 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 mins, 333.8 (1.16 g, 3.48 mmol) in THF (10 mL) was added. Afterstirring at 50° C. for 18 h, the reaction mixture was quenched withwater (40 mL) at 25° C. and extracted with EtOAc (2×50 mL). The combinedorganic phase was washed with water (3×10 mL), brine (30 mL), dried overanhydrous Na₂SO₄, filtered and concentrated in vacuum. The residue waspurified by column (2% of EtOAc in PE) to give 333.9 (620 mg, 54%) as asolid.

1H NMR (400 MHz, CDCl3) δ_(H)4.84 (s, 1H), 4.70 (s, 1H), 2.08-1.57 (m,10H), 1.57-1.06 (m, 13H), 1.06-0.52 (m, 13H).

Synthesis of 333.10

To a solution of 333.9 (2.9 g, 8.77 mmol) in THF (25 mL) was addedBH₃Me₂S (4.38 mL, 43.8 mmol, 10 M) at 0° C. under N₂. After stirring at15° C. for 16 h, the reaction was sequentially treated with EtOH (20mL), NaOH (17.5 mL, 87.7 mmol, 5M) at 0° C., and finally H₂O₂ (8.77 mL,87.7 mmol, 10M) dropwise. After stirring at 60° C. for 1 h, the mixturewas added into water (50 mL) and extracted with EtOAc (3×50 mL). Thecombined organic layer was washed with saturated Na₂S₂O₃ (100 mL), brine(100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by column (0˜30% of EtOAc in PE) to give 333.10(3.2 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.74-3.71 (m, 1H), 3.65-3.61 (m, 0.5H),3.48-3.43 (m, 1H), 3.37-3.33 (m, 0.5H), 1.97-1.57 (m, 8H), 1.57-1.38 (m,8H), 1.38-1.08 (m, 8H), 1.08-0.83 (m, 8H), 0.74-0.58 (m, 5H).

Synthesis of 333.11

To a solution of 333.10 (1 g, 2.86 mmol) in DCM (20 mL) at 0° C. wereadded PPh₃ (1.12 g, 4.29 mmol) and NBS (763 mg, 4.29 mmol) at 25° C.under N₂. After stirring for 2 h, the reaction was diluted with water(20 mL) and extracted with DCM (2×20 mL). The combined organic phase waswashed with brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜5% of EtOAc inPE) to give 333.11 (1.2 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.65-3.62 (m, 0.6H), 3.52-3.49 (m, 0.4H),3.39-3.31 (m, 1H), 2.35 (s, 1H), 1.93-1.44 (m, 10H), 1.44-1.01 (m, 12H),1.01-0.59 (m, 14H).

Synthesis of 333.12 & 335.1

To a solution of 333.11 (1.2 g, 2.91 mmol) in DMF (10 mL) were addedCs₂CO₃ (1.89 g, 5.82 mmol) and 5-methyl-2H-1,2,3,4-tetrazole (489 mg,5.82 mmol) at 25° C. under N₂. After stirring at 80° C. for 16 h, themixture was added into saturated NH₄Cl (20 mL) and extracted with EtOAc(3×20 mL). The combined organic layer was washed with water (2×30 mL),brine (50 mL), dried over anhydrous Na₂SO₄, filtered and concentrated.The residue was purified by column (0˜100% of EtOAc in PE) to give333.12 (700 mg) and 335.1 (300 mg) as solids.

333.12: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.77-4.73 (m, 0.6H), 4.53-4.49 (m,0.4H), 4.31-4.20 (m, 1H), 2.52 (s, 1H), 2.25-1.39 (m, 14H), 1.39-0.91(m, 13H), 0.91-0.55 (m, 12H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₅H₄₃N₄O [M+H]⁺ 415. found 415.

335.1: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.56-4.52 (m, 0.6H), 4.30-4.26 (m,0.4H), 3.89-3.80 (m, 1H), 2.55 (s, 1H), 2.17-1.39 (m, 14H), 1.39-0.94(m, 13H), 0.94-0.59 (m, 12H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₅H₄₃N₄O [M+H]⁺ 415. found 415.

Separation of 333 & 334

333.12 (700 mg, 1.68 mmol) was separated by SFC (Column: DAICELCHIRALCEL OJ-H (250 mm*30 mm, 5 um), Condition: 0.1% NH₃H₂O ETOH, BeginB: 15%, End B: 15%) to give 333 (430 mg, 62%, Rt=1.786 min) and 334 (230mg, 33%, Rt=1.593 min) as solids. The two diastereomers were assignedbased on ¹H NMR of C21-Me (C21-down-Me is at more downfield than C21-upisomer).

333: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.78-4.73 (m, 1H), 4.26-4.20 (m, 1H),2.53 (s, 1H), 2.26-2.17 (m, 1H), 1.90-1.57 (m, 10H), 1.57-1.07 (m, 14H),1.07-0.62 (m, 14H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₅H₄₃N₄O[M+H]⁺ 415, found 415. analytic SFC 98.46% de.

334: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.54-4.49 (m, 1H), 4.31-4.25 (m, 1H),2.53 (s, 1H), 2.19-1.90 (m, 3H), 1.76-1.40 (m, 10H), 1.40-0.95 (m, 14H),0.95-0.60 (m, 12H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₅H₄₃N₄O[M+H]⁺ 415, found 415. analytic SFC 99.82% de.

Separation of 335 & 336

335.1 (300 mg, 0.7235 mmol) was separated by SFC (Column: DAICELCHIRALCEL OD (250 mm*30 mm, 10 um), Condition: 0.1% NH₃H₂O ETOH, BeginB: 30%, End B: 30%) to give 335 (170 mg, 57%, Rt=1.705 min) and 336 (130mg, 43%, Rt=1.574 min) as solids.

335: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.56-4.51 (m, 1H), 3.86-3.80 (m, 1H),2.54 (s, 1H), 2.17-2.07 (m, 1H), 1.91-1.52 (m, 10H), 1.52-1.03 (m, 14H),1.03-0.62 (m, 14H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₅H₄₃N₄O[M+H]⁺ 415 found 415. analytic SFC 98.04% de.

336: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.30-4.26 (m, 1H), 3.89-3.83 (m, 1H),2.54 (s, 1H), 2.09-1.91 (m, 3H), 1.77-1.56 (m, 10H), 1.56-1.11 (m, 10H),1.11-0.79 (m, 11H), 0.79-0.61 (m, 5H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₅H₄₃N₄O [M+H]⁺ 415 found 415. analytic SFC 97.44% de.

Examples 337 & 338: Synthesis of1-((R)-2-((3R,5R,8S,9S,10S,11R,13S,14S,17R)-3,11-dihydroxy-10,13-dimethyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(337) &1-((S)-2-((3R,5R,8S,9S,10S,11R,13S,14S,17R)-3,11-dihydroxy-10,13-dimethyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(338)

Synthesis of 337.1

To a solution of BHT (39.6 g, 179 mmol) in toluene (150 mL) was addedAlMe₃ (2 M in toluene, 44.7 mL, 89.5 mmol) dropwise at 0° C. under N₂.The mixture was stirred at 20° C. for 1 h and used directly as asolution of MAD without further purification. To the fresh prepared MAD(90 mmol) solution was added a solution of 337.0 (10 g, 30.0 mmol) inDCM (80 mL) dropwise at −70° C. under N₂. After stirring at −70° C. for1 h, n-PrMgCl (45 mL, 90 mmol, 2M in ethyl ether) was added dropwise at−70° C. After stirring at −70° C. for another 2 h, the reaction mixturewas poured into saturated aqueous citric acid (200 mL) at 10° C. andextracted with EtOAc (2×100 mL). The combined organic layer was driedover Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜30% of EtOAc in PE) to afford 337.1 (2.8 g, 25%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.97-3.75 (m, 1H), 2.55 (t, J=8.9 Hz, 1H),2.49-2.41 (m, 1H), 2.32 (dd, J=5.0, 11.8 Hz, 1H), 2.22-2.08 (m, 4H),1.93-1.78 (m, 2H), 1.74-1.58 (m, 4H), 1.55-1.30 (m, 10H), 1.29-1.13 (m,6H), 1.06 (s, 3H), 1.00-0.86 (m, 4H), 0.61 (s, 3H).

Synthesis of 337.2

To a suspension of MePh₃PBr (5.28 g, 14.8 mmol) in anhydrous THF (20 mL)was added t-BuOK (2.91 g, 26.0 mmol) at 15° C. under N₂. After stirringat 60° C. for 30 mins, a solution of 337.1 (2.8 g, 7.43 mmol) inanhydrous THF (20 mL) was added dropwise. After stirring for 16 h, themixture was cooled and poured into ice-water (50 mL), stirred for 10mins. and extracted with EtOAc (2×50 mL). The combined organic phase waswashed with brine (2×50 mL), filtered and concentrated. The residue waspurified by flash column (0˜50% of EtOAc in PE) to give 337.2 (2 g,71.9%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.86 (s, 1H), 4.70 (s, 1H), 3.85 (br s,1H), 2.47 (br d, J=14.3 Hz, 1H), 2.18 (dd, J=5.0, 11.8 Hz, 1H),2.10-2.04 (m, 1H), 1.95-1.77 (m, 3H), 1.74-1.48 (m, 9H), 1.47-1.12 (m,13H), 1.10-0.98 (m, 4H), 0.93 (t, J=7.2 Hz, 3H), 0.93-0.80 (m, 1H), 0.55(s, 3H).

Synthesis of 337.3

To a solution of 337.2 (2 g, 5.33 mmol) in DCM (20 mL) were added2,6-dimethylpyridine (2.56 g, 23.9 mmol) and TBSOTf (5.63 g, 21.3 mmol)at 0° C. dropwise under N₂. After stirring at 25° C. for 1 h, thereaction mixture was quenched by water (50 mL) and extracted with DCM(2×30 mL). The combined organic layer was washed with brine (50 mL) anddried over Na₂SO₄, filtered and concentrated in vacuum. The residue waspurified by column (0˜1% of EtOAc in PE) to give 337.3 (1.8 g, 56%) asoil.

Synthesis of 337.4

To a solution of 337.3 (1.8 g, 2.98 mmol) in THF (40 ml) was addedBH₃.Me₂S (0.894 mL, 8.94 mmol) at 0° C. under N₂. After stirring at 25°C. for 16 h, the reaction mixture was cooled to 0° C. and sequentiallytreated with NaOH (5.96 mL, 5M, 29.8 mmol) and then H₂O₂ (2.97 mL, 29.8mmol, 1.13 g/mL, 30% in water) slowly until the reaction temperature nolonger rises and the reaction temperature was maintained below 30° C.After stirring at 50° C. for 1 h, the reaction was quenched withsaturated aqueous Na₂S₂O₃ (30 mL) and stirred at 0° C. for another 1 h.The aqueous phase was extracted with EtOAc (3×30 mL). The combinedorganic phase was washed with saturated Na₂S₂O₃ (2×50 mL), brine (2×50mL), drive over anhydrous Na₂SO₄ filtered and concentrated to give 337.4(1.2 g) as a solid.

Synthesis of 337.5

To a solution of 337.4 (150 mg, 0.2414 mmol) in DMF (5 mL) were addedPh₃P (101 mg, 0.3682 mmol), DEAD (67.2 mg, 0.3862 mmol) and1H-pyrazole-4-carbonitrile (33.7 mg, 0.3621 mmol) at 20° C. under N₂.After stirring for 16 h, the mixture was poured into water (10 mL) andextracted with EtOAc (2×20 mL). The combined organic phase was washedwith brine (10 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated to give 337.5 (120 mg) as a solid.

Synthesis of 337 & 338

To a solution of 337.5 (700 mg, 1.00 mmol) in THF (10 mL) was addedHF-Py (5 mL, 30% in Py) at 20° C. under N₂. After stirring for 16 h, thereaction mixture was neutralized with 5% NaOH (30 mL) and water (20 mL).The mixture was filtered and the filter cake was washed with water (2×10mL) and dried. The residue was purified by flash column (0˜30% of EtOAcin PE) and further purified by SFC (Column DAICEL CHIRALCEL OD (250mm*50 mm, 10 um), Condition 0.1% NH₃H₂O EtOH Begin B: 35 End B: 35Gradient Time(min), 100% B Hold Time(min), FlowRate (ml/min) 70) toafford 338 (70 mg, 11.7%) and 337 (100 mg, 16.7%) as solids. The twodiastereomers were assigned based on 1H NMR of C21-Me (C21-down-Me is atmore downfield than C21-up isomer).

337: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.24 (dd,J=3.8, 13.5 Hz, 1H), 3.97-3.77 (m, 1H), 3.72 (dd, J=9.5, 13.5 Hz, 1H),2.45 (br d, J=14.3 Hz, 1H), 2.25 (dd, J=4.8, 11.9 Hz, 1H), 2.07-1.75 (m,4H), 1.58 (s, 3H), 1.25 (s, 14H), 1.19-1.03 (m, 8H), 0.94 (t, J=7.3 Hz,3H), 0.83 (d, J=6.5 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₉H₄₂N₃[M-2H₂O+H]⁺432.3 found 432.3. SFC: 99% de.

338: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.49 (dd,J=4.5, 13.3 Hz, 1H), 3.89 (br s, 1H), 3.66 (dd, J=10.5, 13.3 Hz, 1H),2.51-2.38 (m, 1H), 2.19 (dd, J=4.9, 11.7 Hz, 1H), 2.11 (br d, J=5.5 Hz,1H), 1.94-1.76 (m, 3H), 1.69-1.57 (m, 4H), 1.45-1.24 (m, 12H), 1.22-1.01(m, 9H), 0.94 (br t, J=7.3 Hz, 3H), 0.79 (s, 3H), 0.68 (d, J=6.5 Hz,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂N₃[M-2H₂O+H]⁺432.3found 432.3. SFC: 99% de.

Example 339:1-((S)-2-((3R,5R,8S,9S,10S,13S,14S,17R)-3-hydroxy-10,13-dimethyl-11-oxo-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(339)

To a solution of 337 (100 mg, 0.2138 mmol) in DCM (10 mL) was addedsilica gel (100 mg) and PCC (91.9 mg, 0.4276 mmol) in at 25° C. underN₂. After stirring at 25° C. for 30 mins, the reaction mixture wasfiltered through a pad of silica and washed with DCM (3×10 mL). Thefiltrate was concentrated. The residue was purified by flash column(0˜30% of EtOAc in PE) to give 339 (31 mg, 31.1%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.74 (s, 1H), 4.33 (dd,J=4.8, 13.5 Hz, 1H), 3.68 (dd, J=9.9, 13.3 Hz, 1H), 2.51 (br d, J=9.5Hz, 1H), 2.45-2.38 (m, 2H), 2.34-2.26 (m, 1H), 2.10 (br s, 1H), 1.75 (brd, J=14.1 Hz, 6H), 1.52-1.21 (m, 15H), 1.18 (s, 3H), 1.06 (dt, J=3.5,14.6 Hz, 1H), 0.94 (t, J=7.3 Hz, 3H), 0.73 (s, 3H), 0.70 (d, J=6.6 Hz,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂N₃O [M−H₂O+H]⁺448.3found 448.3.

Example 340: Synthesis of1-((R)-2-((3R,5R,8S,9S,10S,13S,14S,17R)-3-hydroxy-10,13-dimethyl-11-oxo-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(340)

To a solution of 338 (60 mg, 0.1282 mmol) in DCM (10 mL) was addedsilica (100 mg) and PCC (55.1 mg, 0.2564 mmol) in at 25° C. under N₂.After stirring at 25° C. for 30 mins, the reaction mixture was filteredthrough a pad of silica and washed with DCM (3×10 mL). The filtrate wasconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 340 (4.3 mg, 7.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.23 (dd,J=3.8, 13.6 Hz, 1H), 3.77 (dd, J=9.1, 13.5 Hz, 1H), 2.53-2.46 (m, 2H),2.41 (br d, J=14.5 Hz, 1H), 2.28 (d, J=12.1 Hz, 1H), 2.12-1.94 (m, 2H),1.92-1.66 (m, 5H), 1.52-1.22 (m, 15H), 1.17 (s, 3H), 1.07 (br dd, J=3.4,14.7 Hz, 1H), 0.93 (t, J=7.2 Hz, 3H), 0.79 (d, J=6.6 Hz, 3H), 0.66 (s,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₂N₃O [M−H₂O+H]⁺448.4found 448.4.

Examples 341 & 342: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-cyclopropyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(341) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-cyclopropyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(342)

Synthesis of 341.1

To a solution of A24 (300 g, 1093 mmol) in MeOH (2 L) was added4-methylbenzenesulfonic acid (18.7 g, 109 mmol) at 20° C. under N₂.After stirring at 65° C. for 1 h, the reaction mixture was cooled andthe precipitate was collected by filtration and washed with methanol(2×300 mL) to give 341.1 (230 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.19 (s, 3H), 3.14 (s, 3H), 2.60-2.39 (m,2H), 2.25-2.00 (m, 2H), 1.97-1.90 (m, 2H), 1.86-1.75 (m, 6H), 1.70-1.60(m, 5H), 1.56-1.49 (m, 4H), 1.47-1.35 (m, 10H), 1.30-1.22 (m, 5H),1.15-1.00 (m, 2H), 0.86 (s, 3H).

Synthesis of 341.2

To a suspension of EtPPh₃Br (798 g, 2.15 mol) in THF (1.5 L) was addedt-BuOK (241 g, 2.15 mol) at 20° C. under N₂. After stirring at 50° C.for 30 mins, a solution of 341.1 (230 g, 717 mmol) in THF (500 mL) wasadded. After stirring at 50° C. for 16 h, the mixture was cooled to 25°C., quenched with sat NH₄Cl (500 mL) and extracted with EtOAc (2×500mL). The combined organic phase was washed with brine (2×500 mL), driedover anhydrous Na₂SO₄, filtered and concentrated in vacuum to give theproduct. The solid was suspended in methanol (1 L) and water (1 L). Thesolid was collected by filtration and the filter cake was dried toafford 341.2 (290 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.19 (s, 3H), 3.14 (s, 3H), 2.40-2.10 (m,4H), 1.95-1.35 (m, 13H), 1.33-1.05 (m, 10H), 0.87 (s, 3H).

Synthesis of 341.3

To a solution of 341.2 (275 g, 826 mmol) in THF (2 L) was added 9-BBNdimer (402 g, 1.65 mol) at 20° C. under N₂. After stirring at 50° C. for2 h, the mixture was cooled to 0° C. and sequentially treated withethanol (379 g, 8.26 mol) and NaOH (1.65 L, 5 M, 8.26 mol) dropwise.After the addition was completed, H₂O₂ (825 mL, 8.26 mol, 30%) was addedslowly and the reaction temperature was maintained below 15° C. Afterstirred at 75° C. for 1 h, the reaction was quenched with saturatedaqueous Na₂S₂O₃ (260 mL) and stirred at 0° C. for another 1 h. Themixture was cooled and added into water (2 L). The mixture was filteredand the filter cake was washed with water (3×700 mL), dried under vacuumto give 341.3 (285 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.17-3.09 (m, 6H), 1.96-1.77 (m, 8H),1.64-1.29 (m, 11H), 1.24-0.91 (m, 10H), 0.63 (s, 3H).

Synthesis of 341.4

To a solution of 341.3 (285 g, 813 mmol) in THF (3 L) was added aq HCl(1.62 L, 1.62 mol, 1 M) at 20° C. After stirring for 1 h, the mixturewas treated with water (700 mL) and extracted with DCM (2×500 mL). Thecombined organic phase was washed with brine (2×500 mL), dried overanhydrous Na₂SO₄, filtered, concentrated to afford 341.4 (280 g) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.65 (m, 1H), 2.65-2.55 (m, 1H),2.30-2.10 (m, 1H), 2.00-1.80 (m, 5H), 1.75-1.42 (m, 10H), 1.40-1.28 (m,4H), 1.29-1.15 (m, 7H), 0.66 (s, 3H).

Synthesis of 341.5

To a solution of 341.4 (14 g, 45.9 mmol) in toluene (200 mL) was addedTsOH (787 mg, 4.6 mmol) and ethane-1,2-diol (28.4 g, 458 mol) at 20° C.under N₂. After stirring at 120° C. for 4 h, the mixture was poured intowater (100 mL) and extracted with ethyl acetate (2×100 mL). The combinedorganic layers was washed with brine (100 mL), dried over Na₂SO₄,filtered and concentrated in vacuum. The residue was purified by flashcolumn (5-10% EtOAc in PE) to give 341.5 (8 g, 50%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.93 (s, 4H), 3.76-3.63 (m, 1H), 2.03-1.95(m, 1H), 1.93-1.80 (m, 4H), 1.64-1.58 (m, 3H), 1.56-1.48 (m, 2H),1.44-1.30 (s, 6H), 1.27-0.95 (m, 12H), 0.66 (s, 3H).

Synthesis of 341.6

A solution of 341.5 (10 g, 28.6 mmol) and aq. HCl (28.6 mL, 2 M, 57.2mmol) in THF (30 mL) was stirred at 20° C. for 16 h. The mixture wasadded into saturated NaHCO₃ (200 mL). The aqueous layer was extractedwith EtOAc (3×100 mL). The combined organic layer was washed with brine(150 mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give341.6 (8 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.80-3.70 (m, 1H), 2.59 (t, J=14.0 Hz,1H), 2.25-2.08 (m, 5H), 1.99-1.80 (m, 2H), 1.75-1.30 (m, 13H), 1.28-1.08(m, 7H), 0.69 (s, 3H).

Synthesis of 341.7, 341.7A

To a solution of 341.6 (3.0 g, 9.9 mmol) was addedbromo(cyclopropyl)magnesium (59.0 mL, 29.5 mmol, 0.5 M) in THF (30 mL)at 70° C. under N₂. After stirring at 70° C. for 4 h, the mixture wascooled to 25° C., poured into saturated NH₄Cl (100 mL) and extractedwith DCM (3×50 mL). The combined organic phase was washed with brine(2×100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (5˜25% of EtOAc in PE) to givemixture 341.7A (2.4 g, 70.3%) and 341.7 (700 mg, 20.5%), both as solids.

341.7A: ¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.60 (m, 1H), 1.96-1.78 (m,2H), 1.75-1.49 (m, 8H), 1.43-1.28 (m, 5H), 1.24-1.19 (m, 4H), 1.17-0.98(m, 8H), 0.96-0.79 (m, 2H), 0.66 (s, 3H), 0.38-0.26 (m, 4H).

341.7: ¹H NMR (400 MHz, CDCl₃) δ_(H) 3.77-3.61 (m, 1H), 2.27-2.11 (m,1H), 2.01-1.75 (m, 6H), 1.72-1.36 (m, 14H), 1.34-0.99 (m, 18H),0.90-0.81 (m, 1H), 0.67 (s, 3H), 0.42-0.29 (m, 4H).

Synthesis of 341.8

To a solution of 341.7 (700 mg, 2.01 mmol) in DCM (30 mL) was added DMP(1.27 g, 3.01 mmol) at 20° C. After stirring for 5 mins, the mixture wasquenched with NaHCO₃ (20 mL, saturated) and Na₂S₂O₃ (20 mL, saturated).The organic layer was separated, dried over Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% EtOAc inPE) to give 341.8 (300 mg, 43.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.64-2.48 (m, 2H), 2.21-2.15 (m, 2H), 2.12(s, 3H), 2.10-1.96 (m, 3H), 1.84-1.59 (m, 8H), 1.37-1.07 (m, 11H), 0.62(s, 3H), 0.43-0.32 (m, 4H).

Synthesis of 341.9

To a mixture of MePPh₃Br (1.39 g, 3.9 mmol) in THF (6 mL) was addedt-BuOK (437 mg, 3.90 mmol) at 20° C. under N₂. After stirring at 40° C.for 30 mins, 341.8 (450 mg, 1.30 mmol) was added at 40° C. Afterstirring at 40° C. for 3 h, the mixture was quenched with saturatedNH₄Cl aqueous (50 mL) at 20° C. and extracted with EtOAc (3×50 mL). Thecombined organic phase was washed with water (2×50 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜15% of EtOAc in PE) to give 341.9 (300 mg, 67.4%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.85 (s, 1H), 4.70 (s, 1H), 2.03-1.97 (m,1H), 1.89-1.77 (m, 3H), 1.76 (s, 3H), 1.73-1.63 (m, 4H), 1.52-1.39 (m,6H), 1.37-1.00 (m, 11H), 0.83 (s, 1H), 0.57 (s, 3H), 0.43-0.30 (m, 4H).

Synthesis of 341.10

To a solution of 341.9 (300 mg, 0.9 mmol) in THF (5 mL) was addedBH₃.Me₂S (300 μL, 10 M, 2.99 mmol) at 20° C. under N₂, After stirringfor 1 h, the reaction was sequentially treated dropwise with EtOH (600μL, 10.4 mmol), NaOH (349 mg in 1.75 mL water, 5 M, 8.75 mmol) and H₂O₂(900 μL, 10 M, 9.0 mmol). After stirring at 70° C. for 2 h, the mixturewas quenched by Na₂S₂O₃ (50 mL, 10%) and extracted with EtOAc (50 mL).The organic layer was separated, dried over Na₂SO₄, filtered andconcentrated in vacuum to give 341.10 (300 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.81-3.58 (m, 1H), 3.50-3.33 (m, 1H),1.91-1.76 (m, 4H), 1.51-1.37 (m, 7H), 1.33-1.20 (m, 8H), 1.14-1.01 (m,7H), 0.95 (d, J=6.8 Hz, 2H), 0.91-0.79 (m, 3H), 0.69 (s, 3H), 0.44-0.29(m, 4H).

Synthesis of 341.11

To a solution of 341.10 (300 mg, 0.83 mmol) in DCM (5 mL) were addedN-methylimidazole (101 mg, 1.24 mmol), TEA (251 mg, 2.49 mmol) and TsCl(316 mg, 1.66 mmol) at 20° C. under N₂. After stirring for 0.5 h, themixture was poured into water (20 mL) and extracted with EtOAc (2×30mL). The combined organic phase was washed with water (2×20 mL), driedover anhydrous Na₂SO₄, filtered, concentrated and to give 341.11 (500mg) as oil.

Synthesis of 341.12

To a solution of 341.11 (500 mg, 0.97 mmol) in DMF (10 mL) were addedCs₂CO₃ (632 mg, 1.94 mmol), 4-cyano-pyrazole (180 mg, 1.94 mmol) and KI(161 mg, 0.97 mmol) at 20° C. under N₂. After stirring at 80° C. for 16h, the mixture was diluted with EtOAc (2×30 mL) and washed with water(30 mL), LiCl (5%, 30 mL aq.), dried over Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (5-30% EtOAc inPE)) to give 341.12 (220 mg, 52.0%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.62-4.19 (m,1H), 3.79-3.61 (m, 1H), 2.19-2.06 (m, 1H), 2.03-1.76 (m, 5H), 1.72-1.59(m, 3H), 1.52-1.28 (m, 10H), 1.22-1.03 (m, 7H), 0.87-0.78 (m, 4H),0.73-0.66 (m, 3H), 0.43-0.29 (m, 4H).

Separation of 341 & 342

341.12 (220 mg, 0.5049 mmol) was separated by SFC (column: DAICELCHIRALCEL OD-H (250 mm*30 mm, Sum); Mobile phase: A: CO₂ B: 0.1% NH₃H₂OEtOH; gradient: from 40% to 40% of B, FlowRate (ml/min): 60) to give 341(75.9 mg, 34.6%) and 342 (70 mg, 31.8%) both as solids. 342 (70 mg,0.1606 mmol) was further purified by flash column (0˜30% EtOAc in PE) togive 342 (41.9 mg, 59.5%) as a solid.

341: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.56-4.45(m, 1H), 3.75-3.60 (m, 1H), 2.18-2.06 (m, 1H), 2.04-1.95 (m, 1H),1.92-1.76 (m, 4H), 1.72-1.59 (m, 3H), 1.52-1.19 (m, 12H), 1.16-1.03 (m,5H), 0.85 (s, 1H), 0.80 (s, 3H), 0.64 (d, J=6.5 Hz, 3H), 0.42-0.33 (m,4H). LC-ELSD/MS purity 99%, analytic SFC: 99.82% de, MS ESI calcd. forC₂₈H₄₀N₃ [M−H₂O+H]⁺418.3, found 418.3.

342: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.31-4.21(m, 1H), 3.78-3.67 (m, 1H), 2.08-1.75 (m, 6H), 1.71-1.59 (m, 3H),1.52-1.20 (m, 12H), 1.18-1.03 (m, 6H), 0.81 (d, J=6.8 Hz, 3H), 0.72 (s,3H), 0.42-0.29 (m, 4H). LC-ELSD/MS purity 99%, analytic SFC: 100% de, MSESI calcd. for C₂₈H₄₀N₃[M−H₂O+H]⁺418.3, found 418.3.

Examples 343 & 344: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-isopropyl-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(343) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3-isopropyl-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(344)

Synthesis of 343.1 & 343.1A

To a solution of 341.6 (4 g, 13.1 mmol) in THF (60 mL) was addedCH₂CHMgBr (24.5 mL, 1.6 M, 39.3 mmol) at 0° C. under N₂. After stirringat 15° C. for 2 h, the mixture was added into saturated NH₄Cl (100 mL)and extracted with EtOAc (3×50 mL). The combined organic layer waswashed with brine (100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 343.1 (1.5 g) and 343.1a (800 mg, 18%) both as solids.

343.1a: ¹H NMR (400 MHz, CDCl₃) δ_(H) 5.94 (dd, J=10.8 Hz, 17.6 Hz, 1H),5.23 (dd, J=1.6 Hz, 17.6 Hz, 1H), 5.00 (dd, J=1.6 Hz, 10.8 Hz, 1H),3.80-3.70 (m, 1H), 2.20-2.10 (m, 1H), 1.95-1.59 (m, 9H), 1.52-1.30 (m,6H), 1.28-1.00 (m, 13H), 0.67 (s, 3H). 343.1: ¹H NMR (400 MHz, CDCl₃)δ_(H) 6.12 (dd, J=10.8 Hz, 17.2 Hz, 1H), 5.32 (dd, J=1.2 Hz, 17.6 Hz,1H), 5.15 (dd, J=1.2 Hz, 10.8 Hz, 1H), 3.75-3.65 (m, 1H), 2.00-1.75 (m,6H), 1.70-1.59 (m, 3H), 1.52-1.25 (m, 9H), 1.22 (d, J=6.4 Hz, 3H),1.20-1.00 (m, 8H), 0.66 (s, 3H).

Synthesis of 343.2

To a solution of 343.1 (800 mg, 2.4 mmol) in DCM (20 mL) were addedimidazole (570 mg, 8.4 mmol) and TBSCl (1.08 g, 7.2 mmol) at 15° C.under N₂. After stirring at 15° C. for 16 h, the mixture was filtered.The filtrate was washed with NH₄Cl (50 mL) and the aqueous layer wasextracted with DCM (2×30 mL). The combined organic layer was washed withbrine (100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated.The residue was purified by flash column (0˜5% of EtOAc in PE) to give343.2 (1.2 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 6.12 (dd, J=10.4 Hz, 16.8 Hz, 1H), 5.31(d, J=17.6 Hz, 1H), 5.15 (d, J=10.8 Hz, 1H), 3.80-3.60 (m, 1H),2.00-1.59 (m, 9H), 1.52-0.90 (m, 19H), 0.89-0.87 (m, 9H), 0.64 (s, 3H),0.05-0.04 (m, 6H).

Synthesis of 343.3

To a solution of 343.2 (1 g, 2.2 mmol) and NaHCO₃ (561 mg, 6.7 mmol) inDCM (30 mL) was added m-CPBA (1.35 g, 85%, 6.7 mmol) at 20° C. under N₂.After stirring at 20° C. for 16 h, the mixture was quenched by saturatedNaHCO₃ aqueous (100 mL) and extracted with DCM (2×50 mL). The combinedDCM phase was washed with saturated NaHCO₃/Na₂S₂O₃ aqueous (1:1, 2×100mL), brine (100 mL), dried over Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜15% of EtOAc in PE) to give343.3 (550 mg, 53%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.60 (m, 1H), 3.39 (s, 1H), 2.95-2.85(m, 1H), 2.80-2.70 (m, 1H), 2.10-1.59 (m, 9H), 1.52-1.20 (m, 10H),1.18-0.95 (m, 9H), 0.90-0.85 (m, 9H), 0.65 (s, 3H), 0.05-0.04 (m, 6H).

Synthesis of 343.4

To a solution of 343.3 (550 mg, 1.2 mmol) in THF (10 mL) was addedLiAlH₄ (134 mg, 3.5 mmol) at 20° C. under N₂. After stirring for 1 h,HCl (30 mL, 1.0 M) was added to the mixture and extracted with EtOAc(3×30 mL). The combined organic layer was washed with saturated NaHCO₃(100 mL), brine (100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated to give 343.4 (600 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.10-4.00 (m, 1H), 3.75-3.60 (m, 1H),1.95-1.55 (m, 13H), 1.52-1.28 (m, 8H), 1.25-0.95 (m, 11H), 0.90-0.87 (m,9H), 0.64 (s, 3H), 0.05-0.35 (m, 6H).

Synthesis of 343.5

To a solution of oxalic dichloride (244 mg, 1.9 mmol) in DCM (5 mL) wasadded DMSO (301 mg, 3.9 mmol) at −70° C. under N₂. After stirring for 30minutes, a solution of 343.4 (450 mg, 0.97 mmol) in DCM (5 mL) wasadded. After stirring at −70° C. for 30 minutes. Et₃N (979 mg, 9.7 mmol)was added at −70° C. After stirring at 15° C. for 16 h, the mixture wasadded into water (50 mL) and extracted with DCM (3×20 mL). The combinedorganic layer was washed with brine (50 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜10% of EtOAc in PE) to give 343.5 (280 mg, 63%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.72-3.60 (m, 1H), 2.25 (s, 3H), 1.95-1.59(m, 10H), 1.52-0.95 (m, 18H), 0.90-0.87 (m, 9H), 0.63 (s, 3H), 0.05-0.04(m, 6H).

Synthesis of 343.6

To a solution of MePh₃PBr (1.38 g, 3.9 mmol) in THF (5 mL) was addedt-BuOK (434 mg, 3.9 mmol) at 15° C. under N₂. After stirring at 50° C.for 1 h, 343.5 (300 mg, 0.65 mmol) in THF (5 mL) was added below 15° C.After stirring at 60° C. for 3 h, the mixture was added into saturatedNH₄Cl (100 mL) and extracted with EtOAc (3×30 mL). The combined organiclayer was washed with brine (100 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜10% of EtOAc in PE) to give 343.6 (240 mg, 81%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.01 (d, J=7.6 Hz, 2H), 3.75-3.60 (m, 1H),2.00-1.80 (m, 4H), 1.79 (s, 3H), 1.75-1.59 (m, 4H), 1.52-1.20 (m, 11H),1.18-0.95 (m, 9H), 0.90-0.87 (m, 9H), 0.63 (s, 3H), 0.05-0.04 (m, 6H).

Synthesis of 343.7

To a solution of 343.6 (190 mg, 0.41 mmol) in THF (5 mL) was added Pd/C(50 mg, 10% in water) at 20° C. under H₂. After stirring for 16 h, themixture was filtered and the filtrate was concentrated to give 343.7(190 mg, 100%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.72-3.60 (m, 1H), 2.15-2.05 (m, 1H),1.95-1.59 (m, 9H), 1.52-1.20 (m, 10H), 1.18-0.95 (m, 9H), 0.94-0.87 (m,15H), 0.63 (s, 3H), 0.05-0.04 (m, 6H).

Synthesis of 343.8

To a solution of 343.7 (240 mg, 0.52 mmol) in THF (10 mL) was added TBAF(815 mg, 2.6 mmol) at 20° C. under N₂, After stirring at 55° C. for 16h, the mixture was added into saturated NH₄Cl (100 mL) and extractedwith EtOAc (3×30 mL). The combined organic layer was washed with brine(100 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (0˜50% of EtOAc in PE) to give343.8 (150 mg, 83%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.65 (m, 1H), 2.15-2.05 (m, 1H),2.00-1.60 (m, 8H), 1.52-1.25 (m, 11H), 1.22 (d, J=6.4 Hz, 3H), 1.20-1.00(m, 7H), 0.90 (d, J=0.8 Hz, 3H), 0.88 (d, J=0.8 Hz, 3H), 0.66 (s, 3H).

Synthesis of 343.9

To a solution of 343.8 (150 mg, 0.43 mmol) in DCM was added DMP (364 mg,0.86 mmol) (10 mL). After stirring at 40° C. for 1 h, the mixture wasadded into saturated NaHCO₃ (50 mL) and extracted with DCM (3×20 mL).The combined organic layer was washed with saturated Na₂S₂O₃ (2×50 mL),brine (50 mL), dried over anhydrous Na₂SO₄, filtered and concentrated.The residue was purified by flash column (0˜15% of EtOAc in PE) to give343.9 (140 mg, 94%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.54 (t, J=9.2 Hz, 1H), 2.20-2.12 (m, 1H),2.11 (s, 3H), 2.10-2.00 (m, 2H), 1.90-1.60 (m, 9H), 1.50-1.00 (m, 13H),0.90 (s, 3H), 0.88 (s, 3H), 0.61 (s, 3H).

LC-ELSD/MS 30-90AB_2 min_E, purity 99%, MS ESI calcd. for C₂₃H₃₇₀[M−H₂O+H]+329.3, found 329.3.

Synthesis of 343.10

To a solution of MePh₃PBr (667 mg, 1.9 mmol) in THF (10 mL) was addedt-BuOK (209 mg, 1.9 mmol) at 15° C. under N₂. After stirring at 50° C.for 1 h, 343.9 (130 mg, 0.38 mmol) was added below 50° C. After stirringat 50° C. for 2 h, the mixture was added into saturated NH₄Cl (50 mL)and extracted with EtOAc (3×20 mL). The combined organic layer waswashed with brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtPAcin PE) to give 343.10 (100 mg, 78%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.15-1.95 (m,2H), 1.85-1.59 (m, 12H), 1.52-0.95 (m, 15H), 0.89 (d, J=0.8 Hz, 3H),0.88 (d, J=0.8 Hz, 3H), 0.57 (s, 3H).

Synthesis of 343.11

To a solution of 343.10 (100 mg, 0.29 mmol) in THF (5 mL) was addedBH₃Me₂S (0.29 mL, 10 M, 2.90 mmol) at 20° C. under N₂. After stirring at20° C. for 16 h, the reaction mixture was sequentially treated with EtOH(5 mL) at 15° C., NaOH (4 mL, 5 M, 20.0 mmol) at 0° C., and finally H₂O₂(2.30 g, 20.3 mmol, 30%) dropwise. After stirring at 70° C. for 1 h, themixture was added into water (50 mL) and extracted with EtOAc (3×20 mL).The combined organic layer was washed with saturated Na₂S₂O₃ (2×50 mL),brine (100 mL), dried over anhydrous Na₂SO₄, filtered and concentratedto give 343.11 (100 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.60 (m, 1H), 3.50-3.35 (m, 1H),2.15-1.70 (m, 6H), 1.52-1.17 (m, 16H), 1.15-0.95 (m, 9H), 0.89 (s, 3H),0.88 (s, 3H), 0.68 (s, 3H).

Synthesis of 343.12

To a solution of 343.11 (100 mg, 0.28 mmol) in DCM (10 mL) were addedNBS (98.1 mg, 0.55 mmol) and PPh₃ (144 mg, 0.55 mmol) at 0° C. under N₂.After stirring at 20° C. for 1 h, the mixture was concentrated. Theresidue was purified by flash column (0˜5% of EtOAc in PE) to give343.12 (60 mg, 51%) as colorless oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.65-3.45 (m, 1H), 3.40-3.30 (m, 1H),2.15-2.02 (m, 1H), 1.95-1.59 (m, 9H), 1.52-1.21 (m, 13H), 1.20-0.96 (m,7H), 0.89 (s, 3H), 0.88 (s, 3H), 0.68 (s, 3H).

Synthesis of 343 & 344

To a solution of 343.12 (60 mg, 0.14 mmol) were added Cs₂CO₃ (138 mg,0.42 mmol) and 1H-pyrazole-4-carbonitrile (39.3 mg, 0.42 mmol) at 20° C.under N₂. After stirring at 80° C. for 16 h, the mixture was added intosaturated NH₄Cl (50 mL) and extracted with EtOAc (3×20 mL). The combinedorganic layer was washed with water (2×50 mL), brine (50 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜30% of EtOAc in PE) to give 369.13 (50 mg, 81%) as asolid.

369.13 (50 mg, 0.11 mmol) was separated by SFC (Column: DAICEL CHIRALCELOD-H (250 mm*30 mm, 5m), Condition: 0.1% NH₃H₂O EtOH, Begin B: 35%, EndB: 35%, FlowRate (ml/min): 50) to give 343 (9.1 mg, P1, rt=1.452, 18%)and 344 (17.0 mg, P2, rt=1.646, 34%) both as solids.

343: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.80 (s, 1H), 7.75 (s, 1H), 4.50 (dd,J=4.0 Hz, 12.8 Hz, 1H), 3.65 (dd, J=10.8 Hz, 13.6 Hz, 1H), 2.15-2.05 (m,2H), 1.90-1.59 (m, 10H), 1.52-1.00 (m, 15H), 0.90 (s, 3H), 0.88 (s, 3H),0.79 (s, 3H), 0.68 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, analyticSFC: 99.44% de; MS ESI calcd. for C₂₈H₄₂N₃[M−H₂O+H]+420.3, found 420.3.SFC 100% de.

344: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.25 (dd,J=3.6 Hz, 13.6 Hz, 1H), 3.72 (dd, J=9.2 Hz, 13.2 Hz, 1H), 2.15-1.59 (m,11H), 1.52-1.00 (m, 16H), 0.89 (d, J=1.6 Hz, 3H), 0.87 (d, J=1.2 Hz,3H), 0.80 (d, J=6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%,analytic SFC: 99.24% de; MS ESI calcd. for C₂₈H₄₂N₃[M−H₂O+H]+420.3,found 420.3. SFC 100% de.

Examples 345 & 346: Synthesis of1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17S)-3,17-dihydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(345) &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17S)-3,17-dihydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(346)

Synthesis of 345.1

To a solution of A1 (5 g, 17.2 mmol) in DCM (20 mL) was added Ac₂O (2.98g, 29.2 mmol) and DMAP (2.1 g, 17.2 mmol). After stirring at 20° C. for16 h, the mixture was washed with water (10 mL), NaHCO₃ (10 mL, sat.),dried over Na₂SO₄, filtered and concentrated in vacuum to give 345.1(5.7 g, 100%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.50-2.40 (m, 1H), 2.15-2.00 (m, 1H), 1.97(s, 3H), 1.95-1.70 (m, 7H), 1.65-1.56 (m, 4H), 1.55 (s, 3H), 1.53-1.00(m, 10H), 0.87 (s, 3H).

Synthesis of 345.2

To a suspension of 345.1 (4.7 g, 14.1 mmol) in MeOH (24 mL) were addedNa₂CO₃ (186 mg, 1.76 mmol in 1.8 mL water) and acetone cyanohydrin (6.18g, 72.6 mmol) at 20° C. After stirring at 40° C. for 4 h, water (7.6 mL)was added dropwise at 40° C. and then stirred at 20° C. for 16 h. To themixture was added HCl (24 mL, 0.25 M) and extracted with EtOAc/PE (200mL, 1:1). The organic layer was separated, concentrated and purified byflash column (0˜10% EtOAc in PE) to give 345.2 (4.4 g, 87%, C-17 isomermixture, the ratio is about 3:2) as an oil.

Synthesis of 345.3

To a solution of 345.2 (1.5 g, 4.17 mmol) in THF (10 mL) was addedethoxyethene (3.0 g, 41.7 mmol) and TsOH (7.2 mg, 0.042 mmol). Afterstirring at 30° C. for 2 h, the reaction was treated with TEA (2 drops)and concentrated in vacuum. The residue was purified by flash column(0˜8% EtOAc in PE) to give 345.3 (1.6 g, 89%) as an oil.

Synthesis of 345.4

To a solution of 345.3 (1.5 g, 3.5 mmol) in THF (30 mL) was added MeLi(21.6 mL, 1.6 M, 34.7 mmol). After stirring at 20° C. for 4 h, themixture was quenched by HCl (40 mL, 1 M) and extracted with EtOAc (50mL). The organic layer was separated, dried over Na₂SO₄, filtered,concentrated. The residue was purified by flash column (0˜40% EtOAc inPE) to give 345.4, which was purified by flash column (20-40% EtOAc inPE) to give 345.4 (160 mg).

345: ¹H NMR (400 MHz, CDCl₃) SH, 2.66 (s, 1H), 2.35-2.20 (m, 4H),1.85-1.50 (m, 10H), 1.50-1.30 (m, 9H), 1.25 (s, 3H), 1.20-0.95 (m, 4H),0.93 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₁H₃₁[M+H-2H₂O]⁺299, found 299.

Synthesis of 345.5

To a suspension of MePh₃PBr (29.4 g, 83.2 mmol) in anhydrous THF (50 mL)was added t-BuOK (9.33 g, 83.2 mmol) at 25° C. under N₂. After stirringat 60° C. for 30 min, a solution of 345.4 (3.5 g, 10.4 mmol) inanhydrous THF (10 mL) was dropwise. After stirring for 16 h, the mixturewas cooled, poured into ice-water (50 mL), stirred for 10 min andextracted with EtOAc (2×50 mL). The combined organic phase was washedwith brine (2×50 mL), filtered and concentrated. The residue waspurified by flash column (0˜30% of EtOAc in PE) to give 345.5 (2.8 g,60.4%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.97 (s, 1H), 4.93-4.89 (m, 1H), 2.39(ddd, J=3.5, 11.6, 14.7 Hz, 1H), 1.94-1.70 (m, 12H), 1.53-1.24 (m, 16H),1.23-1.01 (m, 4H), 0.64 (s, 3H)

Synthesis of 345.6

To a solution of 345.5 (300 mg, 0.9021 mmol) in anhydrous THF (5 mL) wasadded BH₃.Me₂S (20.451 mL, 4.51 mmol) at 25° C. under N₂. After stirringat 25° C. for 4 h, the mixture was treated sequentially with EtOH (828mg, 18.0 mmol) at 0° C. and NaOH (3.6 mL, 5M, 18.0 mmol) very slowly.After addition, H₂O₂ (1.8 mL, 18.0 mmol, 10 M in water) was added slowlyuntil the reaction temperature no longer rises and the reactiontemperature was maintained below 30° C. After stirring at 60° C. for 2h, the mixture was added Na₂S₂O₃ (10 mL, sat. aq.) at 0° C. andextracted with EtOAc (3×10 mL). The combined organic phase was washedwith brine (2×20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 345.6 (200 mg, 63.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.18 (br d, J=10.8 Hz, 1H), 3.59 (br d,J=7.8 Hz, 1H), 2.62 (s, 1H), 2.34 (br s, 1H), 1.90-1.60 (m, 11H),1.51-1.23 (m, 13H), 1.17 (d, J=7.3 Hz, 7H), 0.76 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₂₂H₃₅O [M+H-2H₂O]⁺315.3 found 315.3.

Synthesis of 345.7

To a solution of 345.6 (100 mg, 0.2852 mmol) in DCM (5 mL) at 0° C. wereadded PPh₃ (149 mg, 0.5704 mmol) and NBS (100 mg, 0.5704 mmol). Afterstirring at 25° C. for 3 h, the mixture was poured into water (20 mL)and extracted with DCM (3×20 mL). The combined organic phase was washedwith brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 345.7 (65 mg, 55.5%) as colorless oil.

Synthesis of 345 & 346

To a solution of 345.7 (200 mg, 0.4837 mmol) in DMF (1 mL) were addedCs₂CO₃ (253 mg, 0.9674 mmol) and 1H-pyrazole-4-carbonitrile (90.0 mg,0.9674 mmol). After stirring at 80° C. for 1 h, the reaction wascombined with another 4 batches prepared from 50 mg of 380.7respectively. The mixture was added into saturated NH₄Cl (100 mL) andextracted with EtOAc (3×30 mL). The combined organic layer was washedwith water (2×50 mL), LiCl (2×50 mL, 5% in water), brine (50 mL), driedover anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜20% of EtOAc in PE) to give 345 (65 mg) asan oil and 346 (27.1 mg, 13.2%) as solid. 345 was re-purified by SFC(Column DAICEL CHIRALPAK AS (250 mm*30 mm, 10 um) Condition 0.1% NH₃.H₂OEtOH Begin B 30% End B 30% Gradient Time(min) 100% B Hold Time(min)FlowRate (ml/min) δ0) to afford 345 (36.0 mg, 55.4%) as solid.

345: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.75 (s, 1H), 4.47 (dd,J=4.1, 13.7 Hz, 1H), 3.99 (dd, J=10.0, 13.6 Hz, 1H), 2.56-2.31 (m, 1H),2.02-1.58 (m, 12H), 1.48-1.05 (m, 16H), 0.91 (s, 3H), 0.74 (d, J=6.5 Hz,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₆H₃₆N₃[M+H-2H₂O]⁺390.3found 390.3. SFC 100% de.

346: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.75 (s, 1H), 4.47 (dd,J=4.1, 13.7 Hz, 1H), 3.99 (dd, J=10.0, 13.6 Hz, 1H), 2.56-2.31 (m, 1H),2.02-1.58 (m, 12H), 1.48-1.05 (m, 16H), 0.91 (s, 3H), 0.74 (d, J=6.5 Hz,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₆H₃₈N₃O [M+H—H₂O]⁺ 408.3found 408.3. SFC 100% de.

Examples 347 & 348: Synthesis of1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17S)-3,17-dihydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(347) &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17S)-3,17-dihydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(348)

Synthesis of 347.1

To a solution of 347.0 (10 g, 31.3 mmol) in MeOH (60 mL) were addedNa₂CO₃ (414 mg, 3.91 mmol in 4 mL water) and acetone cyanohydrin (14.9g, 175 mmol) at 20° C. under N₂. After stirring at 40° C. under N₂ for 4h, the reaction was treated with water (15 mL) dropwise at 40° C. Afterstirring at 20° C. under N₂ for 16 h, the mixture was treated with HCl(48 mL, 0.25 M) and extracted with EtOAc/PE (3×100 mL, 1:1). Thecombined organic phase was washed with Na₂S₂O₃ (2×130 mL), brine (2×200mL), and concentrated. The residue was purified by flash column (0˜20%EtOAc in PE) to give 347.1 (9.5 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.47-2.30 (m, 2H), 1.96 (br dd, J=6.0,15.1 Hz, 1H), 1.87-1.63 (m, 7H), 1.58-1.23 (m, 16H), 1.22-1.02 (m, 3H),0.98-0.83 (m, 6H).

Synthesis of 347.2

To a solution of 347.1 (9.5 g, 27.4 mmol) in THF (100 mL) was addedethoxyethene (19.7 g, 274 mmol) and TsOH (47.1 mg, 0.2739 mmol). Afterstirring at 30° C. for 2 h, the mixture was treated with TEA (2 drops)and concentrated in vacuum. The residue was purified by flash column(0˜8% EtOAc in PE) to give 347.2 (15 g) as an oil.

Synthesis of 347.3

To a solution of 347.2 (7.5 g, 15.3 mmol) in THF (50 mL) was added MeLi(47.8 mL, 1.6 M, 76.5 mmol) at 0° C. under N₂. After stirring at 25° C.for 4 h, the mixture was quenched with NH₄Cl (40 mL) and extracted withEtOAc (50 mL). The organic layer was separated, dried over Na₂SO₄,filtered, concentrated. The residue was purified by flash column (0˜40%EtOAc in PE) to give 347.3 (5 g) as a solid. To a solution of 347.3 (5g) in MeOH (50 mL) was added HCl (49.3 mL, 1 M, 49.3 mmol) at 25° C.under N₂. After stirring at 25° C. for 1 h, the reaction was extractedwith EtOAc (3×40 mL). The combined organic phase was washed withsaturated aqueous Na₂CO₃ (50 mL), brine (2×50 mL), dried over anhydrousNa₂SO₄, filtered and concentrated to give 347.3 (2.5 g, 70%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.73-2.55 (m, 2H), 2.26 (s, 3H), 1.84-1.59(m, 9H), 1.52-1.24 (m, 15H), 1.22-1.01 (m, 3H), 0.93 (t, J=7.2 Hz, 3H),0.72 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₃H₃₅O[M+H-2H₂O]⁺327.3 found 327.3.

Synthesis of 347.4

To a suspension of MePh₃PBr (19.5 g, 55.1 mmol) in anhydrous THF (50 mL)was added t-BuOK (6.18 g, 55.1 mmol) at 25° C. under N₂. After stirringat 60° C. for 30 min, a solution of 347.3 (2.5 g, 6.89 mmol) inanhydrous THF (30 mL) was dropwise. After stirring at 60° C. for 16 h,the mixture was cooled, poured into ice-water (50 mL), stirred for 10min and extracted with EtOAc (2×50 mL). The combined organic phase waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered,concentrated. The residue was purified by flash column (0˜20% of EtOAcin PE) to give 347.4 (1.5 g, 60%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.91 (s, 1H), 5.00-4.76 (m, 1H), 2.55-2.30(m, 1H), 1.94-1.83 (m, 6H), 1.78-1.61 (m, 6H), 1.53-1.30 (m, 13H),1.29-1.00 (m, 6H), 0.93 (t, J=7.3 Hz, 3H), 0.76-0.61 (m, 3H).

Synthesis of 347.5

To a solution of 347.4 (1.5 g, 4.15 mmol) in anhydrous THF (30 mL) wasadded BH₃.Me₂S (2.07 mL, 20.7 mmol, 10M) at 25° C. under N₂. Afterstirring at 25° C. for 4 h, the reaction was sequentially treated withEtOH (3.81 g, 83.0 mmol) at 0° C. and then NaOH (16.6 mL, 5M, 83.0 mmol)very slowly. After addition, H₂O₂ (8.30 mL, 83.0 mmol, 10 M in water)was added slowly until the reaction temperature no longer rises and thereaction temperature was maintained below 30° C. After stirring at 60°C. for 2 h, the mixture was diluted with Na₂S₂O₃ (50 mL, sat. aq.) at 0°C. and extracted with EtOAc (3×50 mL). The combined organic phase waswashed with brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (20-50% of EtOAcin PE) to give 347.5 (730 mg) as a solid.

Synthesis of 347.6

To a solution of 347.5 (300 mg, 0.7923 mmol) in DCM (10 mL) at 0° C.were added PPh₃ (414 mg, 1.58 mmol) and NBS (278 mg, 1.58 mmol). Afterstirring at 25° C. for 3 h, the mixture was poured into water (20 mL)and extracted with DCM (3×20 mL). The combined organic phase was washedwith brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 347.6 (65 mg, 18.6%) as a solid.

Synthesis of 347.7

To a solution of 347.6 (65 mg, 0.1472 mmol) in DMF (0.5 mL) were addedCs₂CO₃ (77.2 mg, 0.2944 mmol) and 1H-pyrazole-4-carbonitrile (27.4 mg,0.2944 mmol). After stirring at 80° C. for 1 h, the mixture was pouredinto water (20 mL) and extracted with DCM (3×20 mL). The combinedorganic phase was washed with brine (2×50 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The mixture was purified by HPLC(Column YMC Triart C18 150*25 mm*5 um Condition water (10 mMNH₄HCO₃)-ACN Begin B: 73% End B: 100% Gradient Time (min) 9.5; FlowRate(ml/min) 30) to afford 347.7 (15 mg, 22.4%) as solid.

Separation of 347 & 348

347.7 (15 mg, 0.033 mmol) was separated by SFC (Column DAICEL CHIRALCELOD-H (250 mm*30 mm, 5 um) Condition 0.1% NH₃H₂O EtOH Begin B 35%; End B35% Gradient Time(min) 100% B Hold Time(min) FlowRate (ml/min) 50) toafford 348 (2 mg, 13.4%, Rt=min) as solid and 347 (2.5 mg, 16.7%) assolid.

347: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.75 (s, 1H), 4.47 (dd,J=4.1, 13.4 Hz, 1H), 3.99 (dd, J=10.0, 13.6 Hz, 1H), 2.51-2.38 (m, 1H),1.99-1.66 (m, 10H), 1.47-1.24 (m, 14H), 1.22-1.05 (m, 5H), 0.97-0.90 (m,6H), 0.74 (d, J=6.8 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₂₈H₄₀N₃[M+H-2H₂O]⁺418.3 found 418.3. SFC 100% de.

348: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.77 (s, 1H), 4.35 (dd,J=4.3, 14.1 Hz, 1H), 4.04 (dd, J=9.5, 13.8 Hz, 1H), 2.41 (s, 1H),2.03-1.93 (m, 1H), 1.83-1.67 (m, 5H), 1.52-1.43 (m, 9H), 1.38-1.02 (m,13H), 0.93 (t, J=7.2 Hz, 4H), 0.80 (s, 3H), 0.77 (d, J=6.8 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₂N₃O [M+H—H₂O]⁺436.3 found436.3. SFC 100% de.

Examples 349 & 350:1-((R)-2-((3R,5S,8R,9R,10S,13S,14S,17R)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(349) &1-((S)-2-((3R,5S,8R,9R,10S,13S,14S,17R)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(350)

Synthesis of 349.1

To a solution of 329.8 (150 mg, 0.4136 mmol) in DMF (2 mL) were addedPh₃P (173 mg, 0.6617 mmol), DEAD (115 mg, 0.6617 mmol) and1H-pyrazole-4-carbonitrile (57.7 mg, 0.6204 mmol) at 0° C. Afterstirring at 20° C. for 16 h, the mixture was poured into water (10 mL)and extracted with EtOAc (2×20 mL). The combined organic phase waswashed with brine (10 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜15% of EtOAcin PE) to give 349.1 (200 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.35-4.28 (m,1H), 4.21 (q, J=7.2 Hz, 3H), 2.18-2.06 (m, 1H), 1.95-1.70 (m, 5H),1.67-1.60 (m, 5H), 1.55-1.48 (m, 4H), 1.40-1.37 (m, 4H), 1.36-1.31 (m,3H), 1.30-1.25 (m, 6H), 1.22-0.97 (m, 10H), 0.94-0.89 (m, 1H), 0.93-0.89(m, 3H), 0.83-0.78 (m, 3H), 0.73-0.63 (m, 5H).

Separation of 349 & 350

349.1 (200 mg) was separated by SFC (Column: DAICEL CHIRALCEL OJ-H 250mm×30 mm, Sum; Condition: 0.1% NH₃H₂O ETOH; Gradient: from 20% to 20% ofB; Flow rate: 60 mL/min; Column temperature: 40° C.) to afford 349 (79.0mg, 39.6%, Rt=2.418 min) as a solid and 350 (33.0 mg, 16.5%, Rt=2.141min) as a solid.

349: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.51 (dd,J=4.0, 13.2 Hz, 1H), 3.70-3.61 (m, 1H), 2.16-2.05 (m, 1H), 1.90-1.71 (m,4H), 1.67-1.59 (m, 3H), 1.54-1.48 (m, 2H), 1.41-1.28 (m, 7H), 1.27-0.96(m, 11H), 0.94-0.89 (m, 3H), 0.80 (s, 3H), 0.68 (d, J=6.4 Hz, 5H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₄N₃O [M+H]⁺ 438.3 found438.3. SFC 99% de.

350: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (s, 1H), 7.75 (s, 1H), 4.25 (dd,J=4.0, 13.6 Hz, 1H), 3.76-3.68 (m, 1H), 2.07-1.87 (m, 3H), 1.78-1.69 (m,2H), 1.68-1.59 (m, 3H), 1.54-1.48 (m, 2H), 1.38 (d, J=3.6 Hz, 5H),1.34-1.24 (m, 2H), 1.19-0.94 (m, 11H), 0.93-0.89 (m, 3H), 0.81 (d, J=6.4Hz, 3H), 0.71 (s, 1H), 0.70-0.62 (m, 2H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₈H₄₄N₃O [M+H]⁺ 438.3 found 438.3. SFC 99% de.

Examples 351 & 352: Synthesis of1-((S)-2-((3R,5R,8R,9S,10S,13S,14S,17S)-3,17-dihydroxy-3-(methoxymethyl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(351) &1-((R)-2-((3R,5R,8R,9S,10S,13S,14S,17S)-3,17-dihydroxy-3-(methoxymethyl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(352)

Synthesis of 351.1

To a solution of 351.0 (30 g, 99.1 mmol) in methanol (300 mL) was addedMeONa (32.0 g, 594 mmol) at 25° C. After stirring at 60° C. for 16 h,the mixture was poured into water (600 mL) and extracted with EtOAc(2×600 mL). The combined organic layer was washed with saturated Na₂S₂O₃(400 mL), brine (400 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜16% EtOAc inPE) to give 351.1 (12 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.52-3.33 (m, 5H), 2.62 (s, 1H), 2.43 (brdd, J=8.4, 19.2 Hz, 1H), 2.14-2.06 (m, 1H), 1.98-1.68 (m, 7H), 1.62-1.28(m, 13H), 0.98-0.93 (m, 3H), 0.89-0.81 (m, 3H).

Synthesis of 351.2

To a suspension of 351.1 (9.1 g, 27.2 mmol) in MeOH (50 mL) were addedNa₂CO₃ (3.50 mL, 3.40 mmol, 0.97 M) and 2-hydroxy-2-methylpropanenitrile(11.4 g, 135 mmol) at 20° C. After stirring at 40° C. for 4 h, water(14.6 mL) was added dropwise at 40° C. After stirring at 20° C. for 16h, the mixture was quenched with HCl (50 mL, 0.25 M) and extracted withEtOAc/PE (100 mL, 1:1). The combined organic layer was washed withsaturated Na₂S₂O₃ (50 mL, sat.), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜30% ofEtOAc in PE) to give 351.2 (6.24 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.47-3.31 (m, 5H), 2.68-2.61 (m, 1H), 2.59(s, 1H), 2.53-2.38 (m, 1H), 2.15-1.64 (m, 9H), 1.56-1.15 (m, 14H),0.97-0.91 (m, 3H), 0.89-0.79 (m, 3H).

Synthesis of 351.3

To a solution of 351.2 (5.36 g, 14.8 mmol) in THF (35 mL) were addedethoxyethene (10.6 g, 148 mmol) and TsOH (25.4 mg, 0.1480 mmol). Afterstirring at 30° C. for 2 h, the mixture was treated with TEA (7 drops)and concentrated in vacuum. The residue was purified by flash column(0˜8% of EtOAc in PE) to give 351.3 (6.5 g) as soil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.15-4.97 (m, 2H), 3.81-3.45 (m, 11H),3.38-3.26 (m, 4H), 2.61-2.42 (m, 1H), 2.20-1.63 (m, 12H), 1.42-1.14 (m,14H), 0.96-0.94 (m, 3H), 0.93 (d, J=6.4 Hz, 3H), 0.84 (d, J=2.4 Hz, 1H).

Synthesis of 351.4

To a solution of 351.3 (3.3 g, 6.52 mmol) in THF (45 mL) was added MeLi(36.6 mL, 1.6 M, 58.6 mmol) at 0° C. After stirring at 20° C. for 4 h,the mixture was quenched by HCl (40 mL, 1 M) and extracted with EtOAc(50 mL). The organic layer was separated, dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜40% EtOAc in PE) to give 351.4 (1.2 g) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.48-3.30 (m, 5H), 2.70-2.64 (m, 1H), 2.59(br d, J 16.8 Hz, 2H), 2.34-2.17 (m, 3H), 2.04 (s, 1H), 1.98-1.64 (m,7H), 1.55-1.14 (m, 12H), 1.02-0.92 (m, 4H), 0.68 (s, 3H).

Synthesis of 351.5

To a mixture of MePPh₃Br (18.0 g, 50.6 mmol) in THF (100 mL) was addedt-BuOK (5.66 g, 50.6 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 mins, 351.4 (2.4 g, 6.33 mmol) in THF (20 mL) was added at 25° C.After stirring at 60° C. for 3 h, the reaction mixture was cooled,poured into ice water (100 mL) and extracted with EtOAc (100 mL×2). Thecombined organic layer was dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 351.5 (2.13 g) as solid.

¹H NMR (400 MHz, CDCl₃) δ_(H)=4.97 (s, 1H), 4.92-4.88 (m, 1H), 4.81 (d,J=7.2 Hz, 1H), 3.44-3.31 (m, 6H), 2.57-2.49 (m, 1H), 2.38 (ddd, J=3.6,11.6, 14.8 Hz, 1H), 1.98-1.80 (m, 6H), 1.80-1.65 (m, 4H), 1.54-1.30 (m,6H), 1.29-1.05 (m, 5H), 0.98-0.91 (m, 4H), 0.72 (s, 1H), 0.61 (s, 3H).

Synthesis of 351.6 & 351.6a

To a solution of 351.5 (1 g, 2.65 mmol) in THF (35 mL) was added BH₃Me₂S(1.32 mL, 10M, 13.2 mmol) at 25° C. After stirring at 25° C. for 16 h,the reaction was treated with EtOH (2.30 mL, 39.7 mmol) at 25° C., NaOH(7.94 mL, 5.OM, 39.7 mmol) at 0° C., and H₂O₂ (3.98 mL, 39.7 mmol, 30%in water) dropwise. After stirring at 70° C. for 1 h, the reaction wasquenched with saturated aqueous Na₂S₂O₃ (20 mL). After stirring at 0° C.for another 1 h, the reaction was checked by potassium iodide-starchtest paper to confirm excess H₂O₂ was destroyed (did not changed toblue) and extracted with EtOAc (2×30 mL). The combined organic layer waswashed with brine (40 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜50% of EtOAcin PE) to give 351.6 (673 mg) as a solid and 351.6a (132 mg) as oil.

351.6: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.17 (br d, J=10.4 Hz, 1H), 3.57(br d, J=7.2 Hz, 1H), 3.44-3.32 (m, 5H), 3.03-2.91 (m, 1H), 2.63 (br d,J=16.4 Hz, 2H), 2.49 (br s, 1H), 1.99-1.62 (m, 12H), 1.55-1.30 (m, 7H),1.17 (d, J=6.8 Hz, 3H), 1.14-0.99 (m, 2H), 0.98-0.92 (m, 4H), 0.79-0.70(m, 3H).

351.6a: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.23-4.11 (m, 1H), 3.66-3.51 (m,1H), 3.42-3.33 (m, 5H), 2.56 (d, J=2.8 Hz, 2H), 2.37 (dd, J=3.6, 6.1 Hz,1H), 1.98-1.63 (m, 9H), 1.52-1.19 (m, 12H), 1.17 (d, J=6.8 Hz, 3H),1.13-0.97 (m, 2H), 0.94 (s, 3H), 0.74 (s, 3H). LC-ELSD/MS purity 99%, MSESI calcd for C₂₄H₃₉O₂[M-2H₂O+H]⁺359.3, found 359.3, C₂₃H₃₅O[M−MeOH-2H₂O+H]⁺327.3, found 327.3.

Synthesis of 351.7

To a solution of 351.6a (321.5 mg, 0.8134 mmol) in DCM (15 mL) at 0° C.was added PPh₃ (424 mg, 1.62 mmol) and NBS (288 mg, 1.62 mmol). Afterstirring at 25° C. for 30 mins, the reaction was worked up with anotherbather. The mixture was poured into water (40 mL) and extracted with DCM(3×40 mL). The combined organic phase was washed with brine (2×50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜30% of EtOAc in PE) to give 351.7 (650 mg)as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.78-3.68 (m, 1H), 3.44-3.34 (m, 7H), 2.76(s, 1H), 2.57 (br s, 1H), 2.18-2.07 (m, 1H), 1.97-1.75 (m, 5H),1.62-1.36 (m, 15H), 1.17-1.14 (m, 3H), 0.99-0.86 (m, 4H), 0.77 (s, 3H).

Synthesis of 351.8

To a solution of 351.7 (260 mg, 0.5862 mmol) in DMF (5 mL) were addedCs₂CO₃ (459 mg, 1.75 mmol) and 1H-pyrazole-4-carbonitrile (108 mg, 1.17mmol). After stirring at 80° C. for 16 h, the mixture was added intosaturated NH₄Cl (10 mL) and extracted with EtOAc (3×30 mL). The combinedorganic layer was washed with water (2×50 mL), LiCl (2×50 mL, 5% inwater), brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜50% of EtOAcin PE) to give 351.8 (71 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.82-7.79 (m, 1H), 7.77-7.73 (m, 1H),4.52-4.38 (m, 1H), 4.04-3.92 (m, 1H), 3.42-3.33 (m, 5H), 2.68-2.58 (m,1H), 2.52-2.35 (m, 1H), 1.97-1.77 (m, 6H), 1.70-1.37 (m, 15H), 1.21-1.08(m, 2H), 1.03-0.97 (m, 1H), 0.95 (s, 3H), 0.90-0.86 (m, 3H), 0.75-0.70(m, 2H).

Separation of 351 & 352

351.8 (650 mg, 1.38 mmol) was separated by SFC (Column:Phenomenex-Cellulose-2 (250 mm*30 mm, 5 um); Condition: 0.1% NH₃H₂OMEOH) to give 351 (52.8 mg, 8.14%, Rt=2.396 min) as a solid and 352(38.0 mg, 5.86%, Rt=1.992 min) as a solid.

351: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.75 (s, 1H), 4.46 (dd,J=4.0, 13.5 Hz, 1H), 3.98 (dd, J=10.0, 13.4 Hz, 1H), 3.43-3.33 (m, 5H),2.61 (s, 1H), 2.51-2.37 (m, 1H), 2.00-1.62 (m, 9H), 1.54-1.07 (m, 13H),1.03-0.97 (m, 1H), 0.95 (s, 3H), 0.88 (s, 3H), 0.73 (d, J=6.8 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd for C₂₇H₃₆N₃ [M-CH₃OH-2H₂O+H]⁺402.2,found 402.2.

352: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.83 (s, 1H), 7.79 (s, 1H), 4.37 (dd,J=4.0, 13.6 Hz, 1H), 4.05 (dd, J=9.6, 13.6 Hz, 1H), 3.48-3.38 (m, 5H),2.62 (s, 1H), 2.49-2.36 (m, 1H), 2.07-1.62 (m, 10H), 1.55-1.13 (m, 12H),1.05-0.99 (m, 1H), 0.99-0.94 (m, 3H), 0.83-0.73 (m, 6H). LC-ELSD/MSpurity 99%, MS ESI calcd for C₂₈H₄₂N₃O₂ [M−H₂O+H]⁺452.2, found 452.2.

The following examples were synthesized similar to Examples 351 & 352with the listed SM in place of 351.0.

Example SM STRUCTURE Analytical 353 Spiro [estrane- 3,2′- oxiran]-17-one, (5β)-

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.77 (s, 1H), 4.35 (dd, J =3.8, 13.6 Hz, 1H), 4.03 (dd, J = 9.7, 13.7 Hz, 1H), 3.44-3.35 (m, 5H),2.57 (s, 1H), 2.41 (br s, 1H), 2.05-1.93 (m, 1H), 1.86-1.65 (m, 8H),1.52-0.99 (m, 15H), 0.80 (s, 3H), 0.79-0.76 (m, 1H), 0.77 (d, J = 6.8Hz, 2H). LC-ELSD/MS purity 99%, MS ESI calcd for C₂₇H₄₀N₃O₂ [M- H₂O +H]⁺ 438.3, found 438.3, C₂₆H₃₄N₃ [M − CH₃OH − 2H₂O + H]⁺ 388.2 found388.2. 354 Spiro [estrane- 3,2′- oxiran]- 17-one, (5β)-

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.75 (s, 1H), 4.47 (dd, J =4.3, 13.6 Hz, 1H), 3.98 (dd, J = 10.2, 13.9 Hz, 1H), 3.42-3.35 (m, 5H),2.57 (s, 1H), 2.44 (s, 1H), 1.93 (br t, J = 11.9 Hz, 1H), 1.87-1.62 (m,9H), 1.46-1.05 (m, 14H), 0.91 (s, 3H), 0.74 (d, J = 6.8 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd for C₂₇H₄₀N₃O₂ [M − H₂O + H]⁺ 438.3,found 438.3, C₂₆H₃₄N₃ [M − MeOH − 2H₂O + H]⁺ 388.2, found 388.2.

Examples 355 & 356: Synthesis of1-((S)-2-cyano-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(355) &1-((R)-2-cyano-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)-1H-pyrazole-4-carbonitrile(356)

Synthesis of 355.1

To a solution of A1 (3 g, 10.3 mmol) in toluene (50 mL) were addedammonium acetate (2.38 g, 30.9 mmol), acetic acid (6.18 g, 103 mmol) andethyl 2-isocyanoacetate (2.33 g, 20.6 mmol) at 25° C. under N₂. Afterstirring at 140° C. for 18 h, the reaction mixture was quenched withNH₄Cl (50 mL, sat) at 20° C. and extracted with EtOAc (2×50 mL). Thecombined organic phase was washed with brine (100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜20% of EtOAc in PE) to give 355.1 (3.5 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.26 (q, J=7.2 Hz, 2H), 3.23-3.07 (m, 1H),3.02-2.68 (m, 2H), 1.92-1.72 (m, 5H), 1.68-1.37 (m, 11H), 1.36-1.23 (m,12H), 1.22-1.10 (m, 3H), 1.01 (s, 3H)

Synthesis of 355.2

To a solution of 355.1 (500 mg, 1.29 mmol) in MeOH (15 mL) was addedNaBH₄ (488 mg, 12.9 mmol) at 25° C. under N₂. After stirring at 25° C.for 3 h, the reaction mixture was quenched with NH₄Cl (50 mL, sat) at20° C. and extracted with EtOAc (2×50 mL). The combined organic phasewas washed with brine (100 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜20% ofEtOAc in PE) to give 355.2 (433 mg, 97.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.91-3.64 (m, 2H), 2.88-2.79 (m, 1H),2.09-2.03 (m, 1H), 2.03-1.77 (m, 4H), 1.77-1.63 (m, 5H), 1.53-1.36 (m,6H), 1.34-1.17 (m, 8H), 1.15-0.98 (m, 5H), 0.82-0.69 (m, 3H)

Synthesis of 355.3

To a solution of 355.2 (430 mg, 1.24 mmol) in DCM (5 mL) were addedN-methylimidazole (152 mg, 1.86 mmol), TEA (375 mg, 3.72 mmol) and TsCl(472 mg, 2.48 mmol). After stirring at 25° C. for 1 h, the mixture waspoured into NaHCO₃ (10 mL, sat.) and extracted with DCM (2×30 mL). Thecombined organic phase was washed with water (2×20 mL), dried overanhydrous Na₂SO₄, filtered and concentrated to give 355.3 (650 mg) asoil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.85-7.79 (m, 2H), 7.46-7.35 (m, 2H),7.09-7.02 (m, 1H), 6.90-6.85 (m, 1H), 4.09-3.92 (m, 1H), 3.70 (s, 2H),3.05-2.61 (m, 1H), 2.46 (s, 3H), 2.21 (br s, 1H), 2.00-1.74 (m, 4H),1.56-1.33 (m, 7H), 1.26 (s, 7H), 1.14-1.00 (m, 5H), 0.71 (s, 3H),0.24-0.05 (m, 1H)

Synthesis of 355.4

To a solution of 355.3 (650 mg, 1.30 mmol) in DMF (15 mL) were addedCs₂CO₃ (847 mg, 2.60 mmol) and 4-cyano-pyrazole (242 mg, 2.60 mmol).After stirring at 80° C. for 16 h, the mixture was diluted with EtOAc(2×30 mL). The combined organic solution was washed with water (30 mL),LiCl (5%, 30 mL aq.), dried over Na₂SO₄, filtered and concentrated. Theresidue was purified by flash column (5-30% EtOAc in PE) to give 355.4(330 mg, 60.4%) as a solid.

Separation of 355 & 356

355.4 (330 mg, 07845 mmol) was separated by SFC (column:cDAICELCHIRALPAK AD (250 mm*30 mm, 10 um)); Mobile phase: A: CO₂ B: 0.1% NH₃H₂OEtOH; gradient: from 55% to 55% of B, FlowRate (ml/min): 80) to give 355(138 mg) and 356 (91 mg) both as solids. 355 (138 mg, 0.3281 mmol) wasfurther purified by SFC (DAICEL CHIRALPAK AD-H (250 mm*30 mm, 5 um));Mobile phase: A: CO₂ B: 0.1% NH₃H₂O EtOH; gradient: from 45% to 45% ofB, FlowRate(ml/min): 50) to give 355 (93 mg, 67.8%) as a solid.

355: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.99 (s, 1H), 7.85 (s, 1H), 4.47-4.36(m, 1H), 4.26-4.14 (m, 1H), 3.04-2.91 (m, 1H), 2.30-2.20 (m, 1H),2.14-1.94 (m, 1H), 1.89-1.77 (m, 3H), 1.74-1.61 (m, 3H), 1.52-1.36 (m,7H), 1.26 (s, 7H), 1.20-1.00 (m, 6H), 0.77 (s, 3H). LC-ELSD/MS purity99%, analytic SFC: 100% de; MS ESI calcd. for C₂₆H₃₅N₄[M−H₂O+H]⁺403.3found 403.3.

356: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.96 (s, 1H), 7.86 (s, 1H), 4.51-4.35(m, 1H), 3.39-3.26 (m, 1H), 1.97-1.89 (m, 1H), 1.87-1.59 (m, 8H),1.53-1.36 (m, 7H), 1.26 (s, 8H), 1.16-1.01 (m, 5H), 0.81 (s, 3H).LC-ELSD/MS purity 99%, analytic SFC: 100% de; MS ESI calcd. forC₂₆H₃₅N₄[M−H₂O+H]⁺403.3 found 403.3.

Example 357: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-3,3,3-trifluoropropyl)-1H-pyrazole-4-carbonitrile(357)

Synthesis of 357.2

To a solution of 357.1 (1.5 g, 4.30 mmol) in anhydrous THF (30 mL) wasadded CsF (1.62 g, 10.7 mmol) at 0° C. After stirring for 20 mins,TMSCF₃ (1.51 g, 10.7 mmol) was added at 0° C. After stirring for 1 h,TBAF.3H₂O (5.43 g, 17.2 mmol) was added. After stirring at 50° C. foranother 1 h, the reaction mixture was poured into ice-water (50 mL),stirred for 10 mins and extracted with EtOAc (2×30 mL). The combinedorganic phase was washed with brine (2×20 mL), dried over anhydrousNa₂SO₄, filtered and concentrated in vacuum to give 357.2 (1.7 g) as asolid.

Synthesis of 357.3

To a solution of 357.2 (1.7 g, 4.06 mmol) in DCM (50 mL) was added DMP(4.28 g, 10.1 mmol) at 25° C. After stirring at 35° C. for 1 h, thereaction mixture was quenched with saturated NaHCO₃/Na₂S₂O₃ (50 mL/50mL) and extracted with DCM (2×30 mL). The combined organic layer waswashed with aqueous saturated NaHCO₃/Na₂S₂O₃ (50 mL/50 mL), brine (30mL), dried over anhydrous Na₂SO₄, filtered and concentrated to give357.3 (1.25 g) as a solid.

Synthesis of 357.4

To a mixture of MePPh₃Br (10.7 g, 30.2 mmol) in THF (40 mL) was addedt-BuOK (3.38 g, 30.2 mmol) at 25° C. under N₂. After stirring at 50° C.for 1 h, a solution of 357.3 (2.1 g, 5.04 mmol) in THF (8 mL) was addedat 25° C. After stirring at 25° C. for 1 h, the reaction mixture wasquenched with 10% NH₄Cl aqueous (100 mL) at 20° C. and extracted withEtOAc (2×50 mL). The combined organic phase was washed with brine (20mL) dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜10% of EtOAc in PE) to give 357.4 (1.7g, 82%) as an oil

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.87-5.81 (m, 1H), 5.44-5.40 (m, 1H), 3.53(q, J=6.8 Hz, 2H), 3.47-3.37 (m, 2H), 2.68 (s, 1H), 2.32 (t, J=9.6 Hz,1H), 1.98-1.59 (m, 10H), 1.51-1.34 (m, 6H), 1.26-1.11 (m, 8H), 1.09-0.99(m, 2H), 0.63 (s, 3H). ¹⁹F NMR (377 MHz, CDCl₃) δ_(F) −66.89 (s, 3F)

Synthesis of 357.5

To a solution of 357.4 (720 mg, 1.7 mmol) in THF (20 mL) was addedBH₃Me₂S (6.92 mL, 6.92 mmol, 1M in THF) at 0° C. After stirring at 50°C. for 12 h, the reaction mixture was sequentially treated with ethanol(2.42 mL) at 15° C., NaOH aqueous (8.3 mL, 5.0 M) at 0° C. and hydrogenperoxide (4.15 mL, 10 M, 3.07 mmol) dropwise at 0° C. After stirring at78° C. for 1 h, the mixture was cooled to 15° C. and quenched withsaturated aqueous Na₂S₂O₃ (100 mL). The reaction was checked bypotassium iodide-starch test paper to confirm excess H₂O₂ was destroyed(did not changed to blue). The aqueous phase was extracted with EtOAc(2×100 mL). The combined organic phase was washed with brine (2×50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜30% of EtOAc in PE) to afford 357.5 (330 mg,44%) as a solid

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.03-3.96 (m, 1H), 3.91-3.83 (m, 1H), 3.53(q, J=6.8 Hz, 2H), 3.42 (q, J=9.2 Hz, 2H), 2.80-2.51 (m, 1H), 2.23-2.11(m, 1H), 1.95-1.77 (m, 5H), 1.72-1.55 (m, 6H), 1.51-1.32 (m, 8H),1.23-1.00 (m, 9H), 0.72 (s, 3H)

¹⁹F NMR (377 MHz, CDCl₃) δ_(F) −64.17 (s, 3F)

Synthesis of 357

To a solution of 357.5 (80 mg, 0.18 mmol) in DMP were added1H-pyrazole-4-carbonitrile (25.8 mg, 0.28 mmol), DEAD (64.3 mg, 0.37mmol) and PPh₃ (96.8 mg, 0.37 mmol) at 0° C. After stirring at 15° C.for 12 h, the mixture was concentrated. The residue was purified byflash column (0˜30% of EtOAc in PE) to give 357 (70 mg, 75%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.81 (s, 1H), 7.80 (s, 1H), 4.60-4.53 (m,1H), 4.23-4.17 (m, 1H), 3.53 (q, J=7.2 Hz, 2H), 3.47-3.38 (m, 2H),2.95-2.85 (m, 1H), 2.72 (s, 1H), 2.04-1.97 (m, 1H), 1.92-1.74 (m, 4H),1.73-1.57 (m, 6H), 1.52-1.35 (m, 6H), 1.31-1.13 (m, 8H), 1.13-1.06 (m,2H), 0.84 (s, 3H). LC-ELSD/MS: purity 99%; MS ESI calcd. for C₂₈H₃₉F3N₃O[M−H₂O+H]⁺ 490.3, found 490.3.

Example 358: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-13-methyl-17-((R)-1,1,1-trifluoro-3-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(358)

Synthesis of 358.1

To a solution of 357.5 (370 mg, 0.86 mmol) in DCM (5 mL) were addedtriphenylphosphine (335 mg, 1.28 mmol) and CBr₄ (424 mg, 1.28 mmol) at15° C. After stirring at 40° C. for 1 h, the mixture was concentrated.The residue was purified by flash column (0˜30% of EtOAc in PE) to give358.1 (0.26 g, 61%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.73-3.65 (m, 1H), 3.59-3.49 (m, 3H),3.47-3.37 (m, 2H), 2.77-2.58 (m, 1H), 2.56-2.42 (m, 1H), 1.95-1.80 (m,4H), 1.72-1.36 (m, 13H), 1.30-0.99 (m, 10H), 0.71 (s, 3H). ¹⁹F NMR (377MHz, CDCl₃) δ_(F) −64.47 (s, 3F)

Synthesis of 358

To a solution of 358.1 (130 mg, 0.26 mmol) in DMF (3 mL) were added5-methyl-1H-1,2,3,4-tetrazole (44.1 mg, 0.52 mmol) and K₂HPO₄ (119 mg,0.52 mmol) at 20° C. After stirring at 50° C. for 12 h, the mixture wasdiluted with H₂O (10 mL) and extracted with EtOAc (3×10 mL). Thecombined organic phase was washed with brine (5 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜30% of EtOAc in PE) to give 358 (50 mg, 38%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.83-4.73 (m, 2H), 3.53 (q, J=7.2 Hz, 2H),3.42 (q, J=9.2 Hz, 2H), 3.12-3.02 (m, 1H), 2.71 (s, 1H), 2.54 (s, 3H),1.96-1.86 (m, 2H), 1.83-1.75 (m, 3H), 1.73-1.64 (m, 4H), 1.63-1.60 (m,1H), 1.52-1.35 (m, 6H), 1.29-1.13 (m, 8H), 1.12-1.00 (m, 3H), 0.83 (s,3H). LC-ELSD/MS: purity 99%; MS ESI calcd. for C₂₆H₄₀F3N₄O [M−H₂O+H]⁺481.3, found 481.3.

Examples 359 & 360:(3R,5S,8R,9S,10S,13S,14S,17R)-3-ethyl-10,13-dimethyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(359) &(3R,5S,8R,9S,10S,13S,14S,17R)-3-ethyl-10,13-dimethyl-17-((S)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(360)

Synthesis of 359.1

To a solution of Me₃SIO (3.12 g, 14.2 mmol) in DMSO (30 mL) and THF (30mL) was added NaH (340 mg, 14.2 mmol) at 0° C. under N₂. After stirringfor 1 h, 359.0 (3 g, 9.53 mmol) in DMSO (30 mL) was added. Afterstirring at 25° C. for 3 h, the reaction mixture was poured into water(200 mL). The reaction mixture was filtered to give 359.1 (3.3 g) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.64-2.59 (m,2H), 2.06-1.98 (m, 2H), 1.88-1.79 (m, 2H), 1.75 (s, 3H), 1.71-1.65 (m,3H), 1.60-1.52 (m, 3H), 1.35-1.12 (m, 8H), 1.00-0.76 (m, 8H), 0.57 (s,3H).

Synthesis of 359.2

To a solution of 359.1 (4.3 g, 13 mmol) in THF (40 mL) and CuI (371 mg,1.95 mmol) cooled to 0° C. was added MeMgBr (13 mL, 3 M, 39 mmol). Afterstirring at 0° C. for 1 h, the mixture was purified into water (30 mL)and extracted with EtOAc (2×40 mL). The combined organic phase waswashed with brine (40 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜7% of EtOAc inPE) to give 359.2 (3.5 g, 78.2%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (s, 1H), 4.70 (s, 1H), 2.15-1.95 (m,1H), 1.80-1.75 (m, 1H), 1.74-1.60 (m, 4H), 1.59-1.40 (m, 9H), 1.39-1.02(m, 10H), 1.00-0.78 (m, 7H), 0.74 (s, 3H), 0.56 (s, 3H).

Synthesis of 359.3

To a solution of 359.2 (3.5 g, 10.1 mmol) in THF (40 mL) was addedBH₃.Me₂S (5.04 mL, 50.4 mmol, 10 M) dropwise at 0° C. After stirring at25° C. for 3 h, the reaction mixture was cooled to 0° C. andsequentially treated dropwise with ethanol (4.6 g, 100 mmol), NaOHaqueous (15 mL, 151 mmol, 10 M) and finally with H₂O₂ (15 mL, 151 mmol).After stirring at 70° C. for 1 h, the mixture was extracted with EtOAc(2×40 mL). The combined organic phase was washed with saturated Na₂S₂O₃aqueous (2×40 mL), brine (40 mL), dried over Na₂SO₄, filtered andevaporated to give 359.3 (3.5 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.75-3.68 (m, 1H), 3.50-3.42 (m, 1H),1.70-1.60 (m, 8H), 1.59-1.30 (m, 7H), 1.29-1.05 (m, 10H), 1.04-1.00 (m,3H), 0.99-0.78 (m, 6H), 0.77-0.68 (s, 3H), 0.67 (s, 3H).

Synthesis of 359.4

To a solution of 359.3 (3.5 g, 9.65 mmol) in DCM (35 mL) at 0° C. wereadded PPh₃ (3.77 g, 14.4 mmol) and NBS (2.56 g, 14.4 mmol). Afterstirring at 25° C. for 2 h, the reaction mixture was added to water (35mL) and extracted with DCM (2×35 mL). The combined organic phase waswashed with brine (35 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 359.4 (2 g, 48.7%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.65-3.33 (m, 2H), 1.90-1.75 (m, 2H),1.74-1.28 (m, 9H), 1.27-1.15 (m, 7H), 1.14-1.00 (m, 7H), 0.99-0.78 (m,8H), 0.77-0.68 (s, 3H), 0.67 (s, 3H).

Synthesis of 359.5

To a solution of 359.4 (700 mg, 1.64 mmol) in DMF (5 mL) were addedCs₂CO₃ (1.06 g, 3.28 mmol) and 5-methyl-2H-1,2,3,4-tetrazole (275 mg,3.28 mmol). After stirring at 80° C. for 16 h, the mixture was addedinto saturated NH₄Cl (10 mL) and extracted with EtOAc (3×10 mL). Thecombined organic layer was washed with LiCl aq. (5% in water, 2×10 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜35% of EtOAc in PE) to give 359.5 (400 mg,56.8%) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H)4.78-4.20 (m, 2H), 2.53 (s, 3H), 2.26-1.75(m, 2H), 1.70-1.48 (m, 10H), 1.45-1.20 (m, 10H), 1.20-1.03 (m, 5H),1.00-0.85 (m, 3H), 0.74 (s, 3H), 0.70 (s, 3H), 0.69-0.65 (m, 3H).

Separation of 359 & 360

359.5 (567 mg, 1.32 mmol) was separated by SFC (Column: DAICEL CHIRALCELOD (250 mm*30 mm, 10 um), Condition: 0.1% NH₃H₂O ETOH, Begin B:30%, EndB:30%, FlowRate (ml/min):70) to afford 359 (304.1 mg, 53.8%) and 360(184.2 mg, 32.5%) as solids.

359: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.78-4.73 (m, 1H), 4.28-4.20 (m, 1H),2.53 (s, 3H), 2.26-2.20 (m, 1H), 2.00-1.75 (m, 2H), 1.70-1.48 (m, 10H),1.45-1.20 (m, 10H), 1.20-1.03 (m, 4H), 1.00-0.85 (m, 3H), 0.74 (s, 3H),0.70 (s, 3H), 0.69-0.65 (m, 3H). LC-ELSD/MS purity 99%, MS ESI calcd.for C₂₆H₄₅N₄O [M+H]⁺ 429 found 429. SFC 99% de.

360: ¹H NMR (400 MHz, CDCl₃) δ_(H)4.56-4.24 (m, 2H), 2.53 (s, 3H),2.45-1.88 (m, 3H), 1.55-1.51 (m, 2H), 1.50-1.26 (m, 10H), 1.25-1.00 (m,12H), 0.88-0.85 (m, 3H), 0.84-0.80 (m, 3H), 0.73 (s, 3H), 0.71 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₆H₄₅N₄O [M+H]⁺ 429 found 429.SFC 99% de.

Examples 361: Synthesis of(3R,5R,8R,9R,10S,13S,14S,16R,17R)-17-((R)-1-(4-(aminomethyl)-1H-pyrazol-1-yl)propan-2-yl)-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,16-diol(361)

Synthesis of 361.0

To a mixture of EtPPh₃Br (26.5 g, 71.4 mmol) in THF (50 mL) was addedt-BuOK (8.01 g, 71.4 mmol) at 15° C. under N₂. After stirring at 50° C.for 30 min, 313.1 (3.8 g, 11.9 mmol) was added in portions below 40° C.After stirring at 40° C. for another 1 hour to give a suspension, thereaction mixture was quenched with 10% NH₄Cl aqueous (100 mL) at 15° C.and extracted with EtOAc (500 mL). The combined organic phase wasconcentrated under vacuum to give a solid, which was purified bytrituration with MeOH/H₂O (1:1, 300 mL) at reflux to give 361.0 (4.5 g)as oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 5.10 (d, J=7.2 Hz, 1H), 2.41-2.09 (m, 4H),1.78-1.71 (m, 3H), 1.66-1.63 (m, 3H), 1.56-1.51 (m, 3H), 1.50-1.42 (m,3H), 1.37-1.29 (m, 6H), 1.21-1.00 (m, 6H), 0.93 (t, J=7.28 Hz, 3H), 0.87(s, 3H).

Synthesis of 361.1

Under nitrogen gas protection, a 250 mL three-necked flask was chargedwith dry THF (100 mL), 361.0 (5.5 g, 16.6 mmol), AIBN (1.0 g, 6.14 mmol)and (PhS)₂ (1.41 g, 6.46 mmol). After stirring at 65° C. for 24 h, thereaction mixture was poured into EtOAc (200 mL) and washed with NaClO(150 mL). The organic phase was concentrated. The residue was purifiedby flash column (0˜ 10% of EtOAc in PE) to give 361.1 (5.07 g) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.10-4.96 (m, 1H), 2.36-2.14 (m, 2H), 1.72(s, 9H), 1.57-1.38 (m, 10H), 1.30-1.00 (m, 10H), 0.93 (t, J=7.2 Hz, 3H),0.73 (s, 3H).

Synthesis of 361.2

To a stirred solution of (CH₂O)_(n)(1.14 g, 38.2 mmol) in DCM (20 mL)was added Et₂AlCl (63.8 mL, 63.8 mmol, 1M) dropwise at −78° C. Aftermins under N₂, 361.1 (5.07 g, 15.3 mmol) was added at −78° C. Afterstirring at 25° C. for 12 h, the mixture was quenched with ice-water (20mL) at 0° C. and extracted with EtOAc (3×200 mL). The combined organicphase was washed with brine (2×20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column (0˜25% of EtOAc in PE) to give 361.2 (2.68 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.46 (br s, 1H), 3.60-3.50 (m, 1H),3.49-3.40 (m, 1H), 2.46-2.33 (m, 1H), 2.08 (ddd, J=2.8, 6.4, 14.8 Hz,1H), 1.89-1.61 (m, 7H), 1.55-1.28 (m, 15H), 1.28-1.14 (m, 4H), 1.14-1.10(m, 3H), 0.98-0.88 (m, 3H), 0.79 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd for C₂₄H₃₉O₁[M−H₂O+H]⁺343.3, found 343.3.

Synthesis of 361.3

To a solution of 361.2 (1.65 g, 4.57 mmol) in DCM (70 mL) at 0° C. wereadded PPh₃ (2.39 g, 9.14 mmol) and NBS (1.62 g, 9.14 mmol). Afterstirring at 25° C. for 0.5 h, the resulting solution was combined withanother batch prepared from 1 g of 361.2 to work-up. The mixture waspoured into water (20 mL). The aqueous phase was extracted with DCM(3×20 mL). The combined organic phase was washed with brine (2×50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜10% of EtOAc in PE) to give 361.3 (2.37 g)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.51-5.38 (m, 1H), 4.81 (br d, J=2.4 Hz,1H), 3.50 (dd, J=4.0, 9.8 Hz, 1H), 3.18 (t, J=9.2 Hz, 1H), 2.98 (br d,J=15.2 Hz, 1H), 2.59-2.43 (m, 1H), 2.19-2.07 (m, 1H), 1.90-1.63 (m,10H), 1.56-1.43 (m, 9H), 1.42-1.28 (m, 12H), 0.96-0.91 (m, 6H),0.80-0.72 (m, 3H).

Synthesis of 361.4

To a solution of 361.3 (1 g, 2.36 mmol) in DMF (20 mL) were added Cs₂CO₃(1.85 g, 7.08 mmol) and 1H-pyrazole-4-carbonitrile (439 mg, 4.72 mmol).After stirring at 80° C. for 16 h, the reaction was diluted with water(10 mL) and extracted with EtOAc (3×10 mL). The combined organic phasewas washed with water (3×10 mL) brine (30 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by column(5% 30% of EtOAc in PE) to give 361.4 (550 mg) as a solid.

1H NMR (400 MHz, MeOD) δ_(H) 8.36-8.20 (m, 3H), 8.03-7.85 (m, 3H),4.27-4.22 (m, 1H), 4.06-3.99 (m, 1H), 2.80-2.75 (m, 1H), 2.12-2.05 (m,1H), 1.81-1.35 (m, 10H), 1.35-0.97 (m, 15H), 0.92 (t, J=7.2 Hz, 3H),0.81 (s, 3H).

Synthesis of 361

To a solution of 361.4 (550 mg, 1.26 mmol) in THF (20 mL) was addedBH₃.Me₂S (0.63 mL, 6.3 mmol, 10 M) dropwise at 0° C. After stirring at25° C. for 3 h, the resulting colorless suspension was cooled to 0° C.and sequentially treated dropwise at 0° C. with ethanol (579 mg), NaOHaqueous (2.52 mL, 5 M) and H₂O₂ (1.26 mL, 10 M). After stirring at 70°C. for 1 h, the resulting colorless suspension was washed with saturatedNa₂S₂O₃ (50 mL), brine (100 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by pre-HPLC (column: XtimateC18 150×25 mm×5 um, condition: water (0.225% FA)-ACN, Begin B: 70, EndB: 100) to give 361 (100 mg) as oil. 361 (100 mg, 0.2184 mmol) waspurified by SFC (Column: DAICEL CHIRALCEL OD (250 mm×30 mm, 10 um),Condition: 0.1% NH₃H₂O ETOH, Begin B: 40%, End B: 40%) to give 361 (11mg, 92%) as a solid.

1H NMR (400 MHz, DMSO) δ_(H) 7.49 (s, 1H), 7.30 (s, 1H), 4.47 (d, J=5.2Hz, 1H), 4.18-4.13 (m, 1H), 3.99-3.91 (m, 2H), 3.68-3.55 (m, 3H),2.13-2.05 (m, 2H), 1.87-1.54 (m, 8H), 1.54-1.25 (m, 10H), 1.25-0.90 (m,9H), 0.84 (t, J=7.2 Hz, 3H), 0.75 (d, J=6.8 Hz, 3H), 0.69 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₈N₃O₂ [M+H]⁺ 458 found458.

Example 362: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,16R,17R)-3,16-dihydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(362)

To a 10 mL eggplant type Schlenk flask were added CuBr₂ (1.2 mg,0.005462 mmol), 361 (25 mg, 0.05462 mmol) and a solution of NMI (1.34mg, 0.01638 mmol) in DMSO (5 mL). The flask was evacuated and purgedwith oxygen for three times before the flask was attached to a balloonfilled with oxygen. After stirring at 100° C. for 24 h, the reactionmixture was cooled, diluted with water (10 mL) and extracted with EtOAc(3×15 mL). The combined organic solution was concentrated and purifiedby column (10%˜ 60% of PE in EtOAc) to give 362 (2 mg) as a solid.

¹H NMR (400 MHz, DMSO) δ_(H) 7.18 (d, J=3.6 Hz, 2H), 4.36-4.31 (m, 1H),4.24 (t, J=7.2 Hz, 1H), 3.86-3.81 (m, 1H), 2.34-2.24 (m, 1H), 1.82-1.56(m, 10H), 1.56-1.43 (m, 5H), 1.43-1.05 (m, 10H), 1.05-0.85 (m, 9H), 0.74(s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₄N₃O₂ [M+H]⁺ 454found 454.

Example 363: Synthesis of(3R,5R,8R,9R,10S,13S,14S,16R,17R)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,16-diol(363)

Synthesis of 363.1

To a solution of 361.3 (1.72 g, 4.06 mmol) in DMF (5 mL) were addedCs₂CO₃ (3.17 g, 12.1 mmol) and 5-methyl-2H-1, 2, 3, 4-tetrazole (682 mg,8.12 mmol). After stirring at 80° C. for 16 h, the mixture was pouredinto saturated NH₄Cl (10 mL) and extracted with EtOAc (3×30 mL). Thecombined organic layer was washed with water (2×50 mL), LiCl (2×50 mL,5% in water), brine (50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 50% of EtOAcin PE) to give 363.1 (500 mg) as a solid. 363.1 (500 mg, 1.17 mmol) waspurified by SFC (Column: Phenomenex-Cellulose-2 (250 mm*30 mm, Sum);Condition 0.1% NH₃H₂O MEOH) to give 363.1 (280 mg, 56.1%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.50 (br s, 1H), 4.56 (dd, J=4.8, 13.2 Hz,1H), 4.36 (dd, J=9.6, 13.2 Hz, 1H), 2.89 (br s, 1H), 2.54 (s, 3H), 2.07(br d, J=18.4 Hz, 1H), 1.82 (br s, 7H), 1.28 (s, 15H), 1.24-1.11 (m,3H), 1.04 (d, J=6.8 Hz, 3H), 0.93 (t, J=7.2 Hz, 3H), 0.80 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd for C₂₆H₄₁N₄[M−H₂O+H]⁺409.3, found409.3.

Synthesis of 363.2

To a solution of 361.1 (200 mg, 0.4687 mmol) in THF (5 mL) was addedBH₃Me₂S (140 μL, 10 M, 1.40 mmol) at 25° C. After stirring at 25° C. for16 h, the reaction mixture was sequentially treated with EtOH (0.272 mL4.68 mmol) at 25° C., NaOH (0.936 mL, 5.0 M, 4.68 mmol) at 0° C., andH₂O₂ (116 μL, 1.17 mmol, 30% in water). After stirring at 70° C. for an1 h, the reaction was quenched saturated aqueous Na₂S₂O₃ (20 mL) andstirred at 0° C. for another 1 h. The reaction was checked by potassiumiodide-starch test paper to confirm excess H₂O₂ was destroyed (did notchanged to blue). The aqueous phase was extracted with EtOAc (2×30 mL).The combined organic layer was washed with brine (40 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 50% of EtOAc in PE) to give 363.2 (116 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.70 (dd, J=5.2, 13.2 Hz, 1H), 4.34 (dd,J=9.2, 13.2 Hz, 1H), 4.25-4.17 (m, 1H), 2.54 (s, 3H), 2.40 (td, J=7.2,14.4 Hz, 1H), 1.85-1.62 (m, 10H), 1.55-1.43 (m, 7H), 1.42-0.99 (m, 18H),0.97-0.90 (m, 6H), 0.78 (s, 3H).

Synthesis of 363

363.2 (110 mg, 0.2473 mmol) was purified by SFC (Column: DAICELCHIRALPAK AD (250 mm*30 mm, 10 um); Condition: 0.1% NH₃H₂O IPA) to give363 (72.7 mg, 66.6%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.70 (dd, J=4.8, 13.2 Hz, 1H), 4.34 (dd,J=9.2, 13.2 Hz, 1H), 4.20 (br d, J=6.4 Hz, 1H), 2.54 (s, 3H), 2.49-2.36(m, 1H), 1.84-1.60 (m, 7H), 1.54-1.42 (m, 6H), 1.40-1.00 (m, 15H),0.96-0.89 (m, 6H), 0.78 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd forC₂₆H₄₁N₄[M-2H₂O+H]⁺409.3, found 409.3.

Example 364: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17R)-3-hydroxy-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propyltetradecahydro-1H-cyclopenta[a]phenanthren-16(2H)-one(364)

To a solution of 363 (40 mg, 0.08995 mmol) in DCM (3 mL) was added DMP(76.2 mg, 0.1799 mmol). After stirring at 25° C. for 2 h, the mixturewas quenched with sat. NaHCO₃:Na₂S₂O₃ (v:v=1:1, 10 mL) and extractedwith DCM (2×20 mL). The combined organic phase was washed with brine (20mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜ 25% of EtOAc in PE) to give 364 (29.6mg, 74.3%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.67 (dd, J=7.6, 13.1 Hz, 1H), 4.52 (dd,J=7.6, 13.2 Hz, 1H), 2.54 (s, 3H), 2.51-2.40 (m, 1H), 2.23 (dd, J=7.6,17.9 Hz, 1H), 1.88-1.62 (m, 8H), 1.53-1.10 (m, 17H), 1.07 (d, J=6.8 Hz,4H), 0.93 (t, J=7.2 Hz, 3H), 0.86 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd for C₂₆H₄₂N₄₀₂Na [M+Na]⁺465.3, found 465.3, C₂₆H₄₁N₄O[M−H₂O+H]⁺425.4, found 425.4.

Example 365: Synthesis of(3R,5R,8R,9R,10S,13S,14S,16S,17R)-13-methyl-17-((R)-1-(5-methyl-2H-tetrazol-2-yl)propan-2-yl)-3-propylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,16-diol(365)

To a solution of 364 (110 mg, 0.248 mmol) in THF (10 mL) was addedK-selectride dropwise (1.24 mL, 1.24 mmol 1M in THF) at −70° C. underN₂. After the addition, the mixture was allowed to warm to 20° C.slowly. The mixture was quenched with 10% NH₄Cl (30 mL) and extractedwith EtOAc (2×15 mL). The combined organic phase was washed with 10%NH₄Cl (30 mL), dried over Na₂SO₄, filtered, concentrated and purified byflash column (0˜30% of EtOAc in PE) to give 365 (30.2 mg, 27.4%) as asolid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.84 (dd, J=4.2, 13.1 Hz, 1H), 4.45-4.30(m, 2H), 2.70 (s, 1H), 2.54 (s, 3H), 2.39-2.23 (m, 1H), 1.90 (br d,J=12.8 Hz, 1H), 1.83-1.56 (m, 6H), 1.51-1.21 (m, 14H), 1.20-1.09 (m,4H), 1.04 (s, 3H), 0.94 (s, 3H), 0.83 (d, J=6.4 Hz, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd for C₂₆H₄₀N₄[M-2H₂O+H]⁺409.3, found 409.3.

Examples 366 & 367: Synthesis of(3R,5R,8R,9R,10S,13S,14S,17R)-17-((R)-1-(5-ethyl-2H-tetrazol-2-yl)propan-2-yl)-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(366)&(3R,5R,8R,9R,10S,13S,14S,17R)-17-((S)-1-(5-ethyl-2H-tetrazol-2-yl)propan-2-yl)-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-3-ol(367)

Synthesis of 366.1

To a solution of 323.2 (300 mg, 0.827 mmol) in DMF (2 mL) were addedPh₃P (865 mg, 3.30 mmol), DEAD (574 mg, 3.30 mmol) and 5-ethyl-2H-1, 2,3, 4-tetrazole (161 mg, 1.65 mmol). After stirring at 25° C. for 16 h,the mixture was poured into water (10 mL) and extracted with EtOAc (2×20mL). The combined organic phase was washed with brine (10 mL), driedover anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0˜15% of EtOAc in PE) to give 366.1 (170 mg,46.4%) as a solid.

Synthesis of 366 & 367

366.1 (170 mg) was separated by SFC (Column:Chiralcel OD-3 150×4.6 mmI.D., 3 um); Condition: 0.1% NH₃.H₂O ETOH; Gradient: from 40% to 40% ofB; Flow rate: 2.5 mL/min; Column temperature: 35° C.) to afford 367(37.9 mg, Rt=1.289 min) as a solid and 366 (72.7 mg, Rt=2.008 min) as asolid.

366: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.75 (dd, J=4.4, 13.2 Hz, 1H), 4.25(dd, J=10.4, 13.1 Hz, 1H), 2.91 (q, J=8 Hz, 2H), 2.27-2.19 (m, 1H),1.93-1.62 (m, 8H), 1.56-1.51 (m, 2H), 1.49-1.44 (m, 2H), 1.36 (br t,J=8.0 Hz, 13H), 1.27-1.20 (m, 2H), 1.14-1.03 (m, 5H), 0.93 (t, J=7.2 Hz,3H), 0.81 (s, 3H), 0.70 (d, J=6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₇H₄₅N₄ [M−H₂O+H]⁺425.4, found 425.4. SFC 99% de.

367: ¹H NMR (400 MHz, CDCl₃) δ_(H) 4.53 (dd, J=13.3 Hz, 4.0 Hz, 1H),4.29 (dd, J=13.2 Hz, 9.6 Hz, 1H), 2.91 (q, J=8.0 Hz, 2H), 2.10-2.18 (m,1H), 1.91-2.02 (m, 2H), 1.61-1.82 (m, 6H), 1.43-1.49 (m, 3H), 1.21-1.41(m, 14H), 0.98-1.20 (m, 7H), 0.93 t, J=7.3 Hz, 3H), 0.84 (d, J=6.4 Hz,3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₅N₄[M−H₂O+H]⁺425.4, found 425.4. SFC 99% de

Examples 368 & 369: Synthesis of1-((R)-2-((3R,5S,8R,9R,10S,13S,14S,17S)-3,17-dihydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(368) &1-((S)-2-((3R,5S,8R,9R,10S,13S,14S,17S)-3,17-dihydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)-1H-pyrazole-4-carbonitrile(369)

Synthesis of 368.1

A suspension of NaH (228 mg, 60% in oil, 5.72 mmol) in a mixture oft-butanol (2.5 mL) and DMF (3 mL) was stirred under N₂ at −25° C. To thestirred suspension was added triethylphosphite (142 mg, 0.842 mmol) and368.0 (1 g, 2.86 mmol) in a mixture of anhydrous THF (10 mL) and DMF (1mL). After stirring at −25° C. for 2 h, the reaction mixture wasneutralized with acetic acid and diluted with DCM (100 mL) and water (50mL). The aqueous layer was separated and extracted with DCM (2×100 mL).The combined organic layers were washed with brine (100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated under vacuum. The residuewas purified by column (0˜20% EtOAc in PE) to give 368.1 (600 mg) as asolid, which was further purified by SFC (Column: DAICEL CHIRALCEL OD(250 mm×30 mm, 10 um); Condition: 0.1% NH₃H₂O ETOH; Begin B: 20%; End B:20%) to afford 688.1 (180 mg, 45%) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 3.38 (s, 3H), 3.18 (s, 2H), 2.71-2.59 (m,2H), 2.26 (s, 3H), 2.06-1.92 (m, 1H), 1.89-1.59 (m, 10H), 1.49-0.94 (m,10H), 0.72 (s, 5H).

Synthesis of 368.2

To a mixture of MePPh₃Br (1.76 g, 4.93 mmol) in THF (15 mL) was addedt-BuOK (552 mg, 4.93 mmol) at 25° C. under N₂. After stirring at 25° C.for 30 mins, 368.1 (180 mg, 0.493 mmol) in THF (5 mL) was added. Afterstirring at 60° C. for 3 h, the reaction mixture was cooled, poured toNH₄Cl (50 ml) and extracted with EtOAc (2×50 mL). The combined organiclayer was dried over anhydrous sodium sulfate, filtered andconcentrated. The residue was purified by flash column (0˜10% of EtOAcin PE) to give 368.2 (140 mg) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 4.98 (s, 1H), 4.91 (d, J=1.3 Hz, 1H), 3.38(s, 3H), 3.18 (s, 2H), 2.39 (ddd, J=3.4, 11.6, 14.6 Hz, 1H), 1.83 (s,5H), 1.79-1.62 (m, 6H), 1.61-1.60 (m, 1H), 1.54-1.34 (m, 4H), 1.29-0.94(m, 9H), 0.81-0.65 (m, 2H), 0.64 (s, 3H).

Synthesis of 368.3

To a solution of 368.2 (140 mg, 0.386 mmol) in THF (5 mL) was addedBH₃Me₂S (0.0772 mL, 10M, 0.772 mmol) at 25° C. After stirring at 50° C.for 16 h, the reaction mixture was sequentially treated with EtOH (0.337mL, 5.79 mmol) at 0° C., NaOH (1.15 mL, 5.0 M, 5.79 mmol) and H₂O₂(0.577 mL, 5.79 mmol, 30% in water) dropwise. After stirring at 70° C.for 1 h, the mixture was poured into water (30 mL) and extracted withEtOAc (2×30 mL). The combined organic layer was washed with saturatedNa₂S₂O₃ (30 mL), brine (30 mL), dried over anhydrous Na₂SO₄, filteredand concentrated to give 368.3 (50 mg) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 4.06-4.00 (m, 1H), 3.67-3.54 (m, 1H), 3.38(s, 3H), 3.18 (s, 2H), 2.04 (s, 1H), 1.87-1.61 (m, 1H), 1.48-1.34 (m,3H), 1.29-1.11 (m, 10H), 1.29-1.11 (m, 10H), 1.03-0.98 (m, 3H),0.91-0.84 (m, 2H), 0.76 (s, 5H).

Synthesis of 368.4

To a solution of 368.3 (50 mg, 0.131 mmol) in DCM (5 mL) at 0° C. wereadded PPh₃ (68.7 mg, 0.262 mmol) and NBS (46.1 mg, 0.262 mmol). Afterstirring at 25° C. for 2 h, the mixture was poured into water (20 mL)and extracted with DCM (3×20 mL). The combined organic phase was washedwith brine (2×50 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 368.4 (100 mg) as a solid.

1H NMR (400 MHz, CDCl₃) δ_(H) 3.79-3.58 (m, 1H), 3.38 (s, 3H), 3.18 (s,2H), 1.42 (br s, 1H), 1.31-1.20 (m, 6H), 1.11 (br d, J=6.3 Hz, 5H), 0.99(br s, 4H), 0.93-0.83 (m, 4H), 0.81-0.59 (m, 5H), 0.07 (d, J=1.8 Hz,7H), 0.14-0.01 (m, 1H).

Synthesis of 368 & 369

To a solution of 368.4 (100 mg, 0.225 mmol) in DMF (5 mL) were addedCs₂CO₃ (177 mg, 0.675 mmol) and 1H-pyrazole-4-carbonitrile (41.8 mg,0.450 mmol). After stirring at 80° C. for 16 h, the reaction mixture wasadded into saturated NH₄Cl (50 mL) and extracted with EtOAc (3×50 mL).The combined organic layer was washed with LiCl (100 mL, 5% in water),brine (2×100 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by SFC (Column: Welch Xtimate C18150*25 mm*5 um; Condition: water (0.05% NH₃H₂O)-ACN; Begin B: 60%; EndB: 90%) to afford 368 (2.5 mg, 2%) as a solid and 369 (1.1 mg, 1%) as asolid.

368: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.79 (d, J=15.1 Hz, 2H), 4.42-4.27(m, 1H), 4.10-3.95 (m, 1H), 3.39 (s, 3H), 3.18 (s, 2H), 2.46-2.35 (m,1H), 2.03-1.94 (m, 2H), 1.86-1.79 (m, 1H), 1.77-1.69 (m, 4H), 1.68-1.59(m, 5H), 1.29-0.95 (m, 11H), 0.81 (s, 3H), 0.77 (d, J=6.8 Hz, 3H),0.74-0.66 (m, 2H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₀N₃O₂[M−H₂O+H]⁺438.3 found 438.3.

369: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.82-7.72 (m, 2H), 4.54-4.40 (m, 1H),4.05-3.88 (m, 1H), 3.39 (s, 3H), 3.19 (s, 2H), 2.50-2.37 (m, 1H),2.04-1.61 (m, 9H), 1.60 (s, 2H), 1.25 (s, 13H), 0.91 (s, 3H), 0.74 (brd, J=6.5 Hz, 4H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₄₀N₃O₂[M−H₂O+H]⁺438.3 found 438.3.

Examples 400 & 401: Synthesis of5-((S)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)picolinonitrile(401) &5-((R)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-13-methyl-3-propylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)picolinonitrile(400)

Synthesis of 400.1

To a solution of 323.1 (500 mg, 1.45 mmol) in THF (15 mL) was added9-BBN dimer (529 mg, 2.17 mmol) at 15° C. under N₂. After stirring at75° C. for 3 h, 6-bromopyridine-2-carbonitrile (625 mg, 3.42 mmol), CsF(516 mg, 3.42 mmol) and Pd(t-Bu₃P)₂ (87.3 mg, 0.171 mmol) were added.After stirring at 75° C. for 16 h, the mixture was poured into ice-water(50 mL) and extracted with EtOAc (3×40 mL). The combined organic phasewas washed with brine (2×100 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜30% ofEtOAc in PE) to give 400.1 (600 mg) as a solid.

Separation of 400 & 401

400.1 (400 mg, 0.8914 mmol) was separated by SFC (Column DAICELCHIRALPAK AD (250 mm*30 mm, 10 um) Condition 0.1% NH₃H₂O ETOH Begin B55% End B 55% Gradient Time(min) 100% B Hold Time(min) Flow Rate(ml/min) 80) to afford 400 (129.5 mg, 32.3%, Peak 1, Rt=0.860 min) as asolid and 401 (25.6 mg, 6.41%, Peak 2, Rt=2.286 min) as a solid.

400: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.66-7.53 (m, 2H), 2.93(dd, J=3.2, 13.4 Hz, 1H), 2.21 (dd, J=10.4, 13.6 Hz, 1H), 2.09-1.90 (m,2H), 1.83-1.59 (m, 7H), 1.55-1.26 (m, 14H), 1.25-0.99 (m, 7H), 0.93 (t,J=7.2 Hz, 3H), 0.80 (d, J=6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS purity99%, MS ESI calcd. for C₃₀H₄₃N₂[M−H₂O+H]⁺431.3, found 431.3. SFC 99% de.

401: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.71-7.48 (m, 2H), 3.19(dd, J=3.6, 13.6 Hz, 1H), 2.18 (dd, J=11.2, 13.6 Hz, 1H), 1.98 (d,J=12.4 Hz, 1H), 1.91-1.58 (m, 8H), 1.53-1.19 (m, 16H), 1.15-1.00 (m,5H), 0.93 (t, J=7.2 Hz, 3H), 0.79 (s, 3H), 0.69 (d, J=6.4 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₃N₂[M−H₂O+H]⁺431.4, found431.4. SFC 100% de.

Examples 402 & 403: Synthesis of3-((S)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)benzonitrile(402) &3-((R)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)benzonitrile(403)

To a solution of 265.1 (300 mg, 0.831 mmol) in THF (15 mL) under N₂ at15° C. was added 9-BBN dimer (202 mg, 0.831 mmol). After stirring at 75°C. for 3 h, the reaction mixture was cooled to 15° C. and3-bromobenzonitrile (302 mg, 1.66 mmol), CsF (252 mg, 1.66 mmol) andPd(t-Bu₃P)₂ (42.4 mg, 83.1 mmol) were added to the mixture. Afterstirring at 75° C. for 16 h, the reaction was cooled, quenched withwater (30 mL), and extracted with EtOAc (3×30 mL). The combined organiclayer was dried over anhydrous Na₂SO₄, filtered through a pad of silicagel and concentrated. The residue was purified by flash column (0˜30% ofEtOAc in PE) to give a solid. The diastereomers were separated by SFC(Column DAICEL CHIRALPAK AD (250 mm*30 mm, 10 um); Condition 0.1% NH₃H₂OETOH Begin B 55% End B 55% Gradient Time(min) 100% B Hold Time(min)FlowRate(ml/min) 70) to afford 402 (13.1 mg, 4%, Rt=2.613 min) and 403(95.1 mg, 28%, Rt=1.596 min) both as solids. The two diastereomers wereassigned based on ¹H NMR of C21-Me (C21-down-Me is at more downfieldthan C21-up isomer).

402: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.49 (br s, 1H), 7.44 (s, 1H), 7.38(br d, J=4.3 Hz, 2H), 3.56 (q, J=6.6 Hz, 2H), 3.50-3.39 (m, 2H), 3.20(br d, J=13.3 Hz, 1H), 2.73 (br s, 1H), 2.16-2.08 (m, 1H), 2.02 (br d,J=11.5 Hz, 1H), 1.92-1.65 (m, 4H), 1.53-1.27 (m, 12H), 1.25-1.08 (m,11H), 0.82 (s, 3H), 0.70 (br d, J=6.3 Hz, 3H). LC-ELSD/MS purity 99%, MSESI calcd. for C₃₁H₄₅NO₂Na[M+Na]⁺486.3 found 486.3. SFC: 100% de.

403: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.49-7.45 (m, 1H), 7.42 (s, 1H), 7.36(d, J=5.0 Hz, 2H), 3.53 (q, J=7.0 Hz, 2H), 3.42 (q, J=9.3 Hz, 2H), 2.91(dd, J=2.9, 13.4 Hz, 1H), 2.70 (s, 1H), 2.11 (dd, J=10.5, 13.6 Hz, 1H),2.04-1.91 (m, 2H), 1.87-1.61 (m, 7H), 1.52-1.26 (m, 8H), 1.24-1.01 (m,11H), 0.78 (d, J=6.3 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MSESI calcd. for C₃₁H₄₅NO₂Na[M+Na]⁺486.3 found 486.3. SFC: 100% de.

Examples 404 & 405: Synthesis of5-((S)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)picolinonitrile(404) &5-((R)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)picolinonitrile(405)

Synthesis of 404.1

To solution of B22 (500 mg, 1.44 mmol) in THF (15 mL) under nitrogenatmosphere at 15° C. was added 9-BBN dimer (524 mg, 2.15 mmol). Afterstirring at 55° C. for 3 h, 5-bromopyridine-2-carbonitrile (523 mg, 2.86mmol), CsF (431 mg, 2.86 mmol) and Pd(t-Bu₃P)₂ (72.9 mg, 0.1430 mmol)were added to the mixture. After stirring at 55° C. for 16 h, themixture was quenched by H₂O (40 mL) and extracted with EtOAc (50 mL).The organic layer was separated, dried over Na₂SO₄, filtered,concentrated and purified by flash column (0˜ 25% EtOAc in PE) to give404.1 (85 mg) as a solid.

LC-ELSD/MS purity 99%, MS ESI calcd for C₂₉H₄₁N₂O [M−H₂O+H]⁺450.3, found450.3.

Synthesis of 404 & 405

404.1 (480 mg, 0.9723 mmol) was purified by SFC (Column DAICEL CHIRALPAKAD (250 mm*30 mm, 10 um); Condition 0.1% NH₃H₂O IPA) to give 404 (17.6mg, 7.04%, Rt=2.394 min) and 405 (142.1 mg, 56.8%, Rt=0.842 min) both assolids.

404: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.63-7.49 (m, 2H),3.43-3.35 (m, 5H), 3.27-3.12 (m, 1H), 2.60 (s, 1H), 2.27-2.10 (m, 1H),1.98 (br d, J=12.4 Hz, 1H), 1.91-1.61 (m, 8H), 1.45-1.05 (m, 16H), 0.79(s, 3H), 0.69 (d, J=6.8 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd forC₂₉H₄₁N₂O[M−H₂O+H]⁺433.3, found 433.3.

405: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.63-7.49 (m, 2H),3.43-3.33 (m, 5H), 2.93 (dd, J=3.2, 13.3 Hz, 1H), 2.59 (s, 1H), 2.20(dd, J=10.4, 13.6 Hz, 1H), 2.11-1.64 (m, 8H), 1.46-0.95 (m, 17H), 0.80(d, J=6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd forC₂₉H₄₁N₂O[M−H₂O+H]⁺433.3, found 433.3.

Examples 406 & 407: Synthesis of5-((R)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)nicotinonitrile(406) &5-((S)-2-((3R,5R,8R,9R,10S,13R,14S,17R)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)nicotinonitrile(407)

Synthesis of 406.1

To solution of H1 (400 mg, 1.26 mmol) in dioxane (10 mL) under nitrogenatmosphere at 15° C. was added 9-BBN dimmer (461 mg, 1.89 mmol). Afterstirring 75° C. for 3 h, 5-bromopyridine-3-carbonitrile (461 mg, 2.52mmol), CsF (380 mg, 2.52 mmol) and Pd(t-Bu₃P)₂ (64.3 mg, 126 μmol) wereadded. After stirring at 75° C. for 16 h, the reaction was cooled,quenched with water (30 mL), and extracted with EtOAc (3×30 mL). Thecombined organic layer was dried over anhydrous sodium sulfate, filteredand concentrated to give the product. The product was purified by flashcolumn (0˜50% of EtOAc in PE) to give 406.1 as a solid.

Separation of 406 & 407

406.1 was separated by SFC (Column: DAICEL CHIRALCEL OJ-H (250 mm*30 mm,5 um)); condition: 0.1% NH₃H₂O ETOH IPA; Begin B: 20%; End B: 30%;) toafford 406 (122.8 mg, 40.8%, P1, Rt=2.761 min) 407 (38.2 mg, 12.7%, P2,Rt=3.092 min) both as solids.

406: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.71 (d, J=1.6 Hz, 1H), 8.59 (d,J=2.0 Hz, 1H), 7.72 (t, J=1.6 Hz, 1H), 2.91 (dd, J=2.8, 14.6 Hz, 1H),2.19 (dd, J=10.4, 14.0 Hz, 1H), 2.06-1.91 (m, 2H), 1.88-1.78 (m, 3H),1.72-1.59 (m, 4H), 1.52-1.33 (m, 8H), 1.31-1.09 (m, 12H), 0.81 (d, J=6.8Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS: purity>99%, analytic SFC: 98.36% de,MS ESI calcd. for C₂₈H₄₁N₂₀ [M+H]⁺ 421.3, found 421.3.

407: ¹H NMR (400 MHz, CDCl₃) δ_(H) 8.71 (d, J=1.6 Hz, 1H), 8.59 (d,J=1.6 Hz, 1H), 7.72 (s, 1H), 3.17 (dd, J=3.6, 13.6 Hz, 1H), 2.16 (dd,J=11.2, 13.6 Hz, 1H), 2.02-1.96 (m, 1H), 1.89-1.79 (m, 4H), 1.71-1.59(m, 4H), 1.51-1.35 (m, 10H), 1.31-1.15 (m, 10H), 0.80 (s, 3H), 0.70 (d,J=6.4 Hz, 3H) LC-ELSD/MS: purity>99%, analytic SFC: 96.36% de, MS ESIcalcd. for C₂₈H₃₉N₂[M−H₂O+H]⁺403.3, found 403.3.

The following examples were synthesized similar to Examples 400-407 withthe listed bromide and appropriate SM. The diastereomers were assignedbased on 1H NMR of C21-Me.

Example SM Br STRUCTURE Analytical 410 323.1 5- bromo- 2-(trifluoromethyl) pyridine

¹H NMR (400 MHz, CDCl₃)) δ_(H) 8.50 (s, 1H), 7.77-7.47 (m, 2H), 3.20(dd, J = 3.6, 13.8 Hz, 1H), 2.19 (dd, J = 11.2, 13.4 Hz, 1H), 2.01 (brd, J = 12.4 Hz, 1H), 1.92-1.64 (m, 7H), 1.50-1.25 (m, 15H), 1.18-1.00(m, 6H), 0.93 (t, J = 7.2 Hz, 4H), 0.80 (s, 3H), 0.70 (d, J = 6.4 Hz,3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₃F₃N [M − H₂O + H]⁺474.3, found 474.3. SFC 100% de. 411

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.69-7.54 (m, 2H), 2.93 (dd,J = 2.8, 13.6 Hz, 1H), 2.21 (dd, J = 10.4, 13.6 Hz, 1H), 2.08-1.91 (m,2H), 1.85- 1.59 (m, 7H), 1.55-1.23 (m, 15H), 1.22- 0.98 (m, 7H), 0.93(t, J = 7.2 Hz, 3H), 0.81 (d, J = 6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₃₀H₄₃F₃N [M − H₂O + H]⁺ 474.3, found474.3. SFC 100% de. 412 265.1 6- bromopicolinonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.74- 7.65 (m, 1H), 7.51 (d, J = 7.5 Hz,1H), 7.31 (d, J = 8.0 Hz, 1H), 3.53 (q, J = 7.0 Hz, 2H), 3.43 (q, J =9.3 Hz, 2H), 3.04 (dd, J = 3.4, 13.4 Hz, 1H), 2.69 (br s, 1H), 2.37 (dd,J = 10.4, 13.2 Hz, 1H), 2.03-1.59 (m, 10H), 1.51-1.25 (m, 8H), 1.23-0.93(m, 11H), 0.80 (d, J = 6.5 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%,MS ESI calcd. for C₃₀H₄₅N₂O₂ [M + H]⁺ 465.3 found 465.3. SFC: 100% de.413 265.1 6- bromopicolinonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.74- 7.62 (m, 1H), 7.50 (d, J = 7.5 Hz,1H), 7.31 (d, J = 7.8 Hz, 1H), 3.53 (q, J = 7.0 Hz, 2H), 3.43 (q, J =9.2 Hz, 2H), 3.28 (dd, J = 4.0, 13.2 Hz, 1H), 2.70 (s, 1H), 2.38 (dd, J= 10.8, 13.2 Hz, 1H), 1.99 (br d, J = 13.2 Hz, 2H), 1.92-1.71 (m, 4H),1.67-1.60 (m, 3H), 1.51-1.29 (m, 8H), 1.28-1.02 (m, 11H), 0.80 (s, 3H),0.69 (d, J = 6.5 Hz, 3H). LC- ELSD/MS purity 99%, MS ESI calcd. forC₃₀H₄₅N₂O₂ [M + H]⁺ 465.3 found 465.3. SFC: 100% de. 414 265.1 3- bromo-6- methylpicolinonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.48 (d, J = 8.0 Hz, 1H), 7.28 (s, 1H),3.53 (q, J = 6.9 Hz, 2H), 3.43 (q, J = 9.1 Hz, 2H), 3.28 (dd, J = 4.1,13.4 Hz, 1H), 2.70 (br s, 1H), 2.56 (s, 3H), 2.35 (dd, J = 11.2, 13.4Hz, 1H), 2.02 (br d, J = 12.3 Hz, 1H), 1.90-1.61 (m, 8H), 1.52-1.18 (m,15H), 1.13-1.04 (m, 4H), 0.84 (s, 3H), 0.70 (d, J = 6.5 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₁H₄₅N₂O [M + H − H₂O]⁺ 461.3found 461.3. SFC: 99% de. 415 265.1 3- bromo- 6- methylpicolinonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.51 (d, J = 8.0 Hz, 1H), 7.30 (s, 1H),3.55 (q, J 7.0 Hz, 2H), 3.45 (q, J = 9.1 Hz, 2H), 3.16-3.02 (m, 1H),2.72 (br s, 1H), 2.59 (s, 3H), 2.33 (dd, J = 10.9, 13.7 Hz, 1H),2.10-1.96 (m, 2H), 1.89-1.61 (m, 8H), 1.23 (br t, J = 7.0 Hz, 18H), 0.83(d, J = 6.5 Hz, 3H), 0.72 (s, 3H). LC- ELSD/MS purity 99%, MS ESI calcd.for C₃₁H₄₅N₂O [M + H − H₂O]⁺ 461.3 found 461.3. SFC: 99% de. 416 265.12- bromobenzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.59 (s, 1H), 7.49 (s, 1H), 7.30-7.27 (m,1H), 7.25 (br s, 1H), 3.53 (q, J = 6.9 Hz, 2H), 3.43 (q, J = 9.2 Hz,2H), 3.13 (dd, J = 3.0, 13.3 Hz, 1H), 2.31 (dd, J = 11.2, 13.2 Hz, 1H),2.09-1.91 (m, 2H), 1.86-1.57 (m, 10H), 1.52-1.17 (m, 14H), 1.16-0.94 (m,6H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₃₈N [M −EtOH − H₂O + H]⁺ 400.3 found 400.3. SFC: 100% de. 417 265.1 2-bromobenzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.62 (d, J = 7.4 Hz, 1H), 7.54-7.48 (m,1H), 7.32-7.29 (m, 1H), 7.27 (s, 1H), 3.56 (q, J = 7.0 Hz, 2H), 3.45 (q,J = 9.2 Hz, 2H), 3.34 (dd, J = 4.2, 13.4 Hz, 1H), 2.72 (s, 1H),2.49-2.34 (m, 1H), 2.06 (br d, J = 12.1 Hz, 1H), 1.97-1.60 (m, 9H),1.53-1.26 (m, 9H), 1.23 (t, J = 6.9 Hz, 4H), 1.20-1.01 (m, 5H), 0.87 (s,3H), 0.71 (d, J = 6.5 Hz, 3H). LC- ELSD/MS purity 99%, MS ESI calcd. forC₂₉H₃₈N [M −EtOH −H₂O + H]⁺ 400.3 found 400.3. SFC: 100% de. 418 323.12- bromo- 5- methylpyrazine

¹H NMR (400 MHz, CDCl₃) δ_(H) 0.71 (s, 3H) 0.82 (d, J = 6.53 Hz, 3H)0.93 (t, J = 7.28 Hz, 3H) 1.13-0.99 (m, 5H) 1.25- 1.13 (m.3H) 1.34 (brs,7H) 1.46-1.36 (m, 5H) 1.55-1.4 (m, 4H) 1.74-1.67 (m, 1H) 1.81-1.74 (m,2H) 1.91-1.81 (m, 1H) 1.99-1.91 (m, 2H) 2.32 (dd, J = 13.43, 10.16 Hz,1H) 2.52 (s, 3H) 3.00-2.94 (m. 1H) 8.27 (d, J = 1.25 Hz, 1H) 8.38 (s,1H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₇N₂O [M +H]⁺ 439.4found 439.4. 419

LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₉H₄₇N₂O [M + H]⁺ 439.4 found439.4. 420 B22 5- bromo- 2- (trifluoromethyl) pyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.60 (s, 2H), 3.43-3.33 (m,5H), 3.20 (br d, J = 16.8 Hz, 1H), 2.60 (s, 1H), 2.19 (dd, J = 11.2,13.6 Hz, 1H), 2.01 (br d, J = 12.8 Hz, 1H), 1.92-1.60 (m, 9H), 1.42-1.05(m, 15H), 0.84-0.84 (m, 1H), 0.80 (s, 3H), 0.70 (d, J = 6.4 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd for C₂₉H₄₁F₃NO [M − H₂O + H]⁺ 476.3,found 476.3. 421

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.60 (s, 2H), 3.43-3.33 (s,5H), 2.98-2.90 (m, 1H), 2.64-2.55 (m, 1H), 2.25-2.16 (m, 1H), 2.08-1.91(m, 2H), 1.87-1.59 (m, 8H), 1.49-0.93 (m, 15H), 0.81 (d, J = 6.4 Hz,3H), 0.70 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd for C₂₉H₄₁F₃NO [M−H₂O + H]⁺ 476.3, found 476.3. 422 B22 2- bromo- 5- methylpyrazine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.37 (s, 1H), 8.27 (s, 1H), 3.43-3.35 (m,5H), 3.21 (br d, J = 10.0 Hz, 1H), 2.59 (s, 1H), 2.52 (s, 3H), 2.37-2.26(m, 1H), 2.06-1.74 (m, 7H), 1.42-0.96 (m, 18H), 0.81 (s, 3H), 0.70 (d, J= 6.4 Hz, 3H). LC-ELSD/MS purity 99%, MS ESI calcd for C₂₈H₄₅N₂O₂ [M +H]⁺ 441.3, found 441.3. 423

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.40 (s, 1H), 8.30 (s, 1H), 3.45-3.38 (m,5H), 2.99 (dd, J = 2.8, 13.2 Hz, 1H), 2.60 (s, 1H), 2.55 (s, 3H), 2.34(dd, J = 10.4, 13.4 Hz, 1H), 2.07-1.68 (m, 7H), 1.53- 0.96 (m, 18H),0.84 (d, J = 6.4 Hz, 3H), 0.73 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd for C₂₈H₄₅N₂O₂ [M + H]⁺ 441.3, found 441.3. 424 B22 2,3- dichloro-6- methylpyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.47 (d, J = 8.4 Hz, 1H), 6.91 (d, J = 8.4Hz, 1H), 3.43-3.36 (m, 5H), 3.33 (dd, J = 4.7, 12.8 Hz, 1H), 2.60-2.52(m, 2H), 2.50 (s, 3H), 2.19-1.99 (m, 2H), 1.91-1.59 (m, 7H), 1.50-1.03(m, 16H), 0.82 (s, 3H), 0.69 (d, J = 6.6 Hz, 3H). LC-ELSD/MS purity 99%,MS ESI calcd. for C₂₉H₄₅ClNO₂ [M + H]⁺ 474.3 found 474.3. SFC: 100% de.425

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.48 (d, J = 8.4 Hz, 1H), 6.91 (d, J = 8.0Hz, 1H), 3.43-3.36 (m, 5H), 3.03 (dd, J = 4.7, 12.8 Hz, 1H), 2.70-2.50(m, 2H), 2.50 (s, 3H), 2.30-2.20 (m, 1H), 1.85-1.59 (m, 13H), 1.56-1.03(m, 18H), 0.80 (s, 3H), 0.75 (d, J = 6.8 Hz, 3H). LC- ELSD/MS purity99%, MS ESI calcd. for C₂₉H₄₅ClNO₂ [M + H]⁺ 474.3 found 474.3. SFC: 100%de. 426 305.2 5- bromo- 2- (trifluoromethyl) pyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1 H), 7.57-7.65 (m, 2 H),2.98-2.88 (m, 1 H), 2.26-2.16 (m, 1 H), 1.56-2.06 (m, 8 H), 1.35-1.50(m, 8 H), 1.00-1.26 (m, 12 H), 0.94 (s, 3 H), 0.81 (d, J = 6.4 Hz, 3 H),0.68 (s, 3 H). LC-ELSD/MS: purity 99%, analytic SFC: 100% de; MS ESIcalcd. for C₂₉H₄₁F₃N [M − H₂O + H]⁺ 460.3, found 460.3. 427

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1 H), 7.57-7.64 (m, 2 H),3.25-3.10 (m, 1 H), 2.24-2.13 (m, 1 H), 1.63-2.06 (m, 8 H), 1.37-1.50(m, 8 H), 1.05-1.26 (m, 12 H), 0.96 (s, 3 H), 0.78 (s, 3 H), 0.70 (d, J= 6.4 Hz, 3 H). LC-ELSD/MS: purity 99%, analytic SFC: 99% de; MS ESIcalcd. for C₂₉H₄₁F₃N [M − H2O + H]⁺ 460.3, found 460.3. 428 H1 (2-bromothiazol- 5- yl) methanol

¹H NMR(400 MHz, CDCl₃) δ_(H) 7.03 (s, 1H), 4.74 (s, 2H), 3.36 (dd, J =3.6, 14.4 Hz, 1H), 2.64 (dd, J = 10.0, 13.6 Hz, 1H), 2.29-2.20 (m, 1H),2.02-1.92 (m, 2H), 1.89-1.76 (m, 4H), 1.51-1.33 (m, 8H), 1.32-1.02 (m,15H), 0.83 (d, J = 6.4 Hz, 3H), 0.78 (s, 3H)^(.) LC-ELSD/MS: purity >99%, analytic SFC: 100% de. MS ESI calcd. for C₂₆H₄₂NO₂S [M + H]⁺ 432.3,found 432.3. 429

¹H NMR(400 MHz, CDCl₃) δ_(H) 7.03 (s, 1H), 4.74 (d, J = 6.0 Hz, 2H),3.12 (dd, J = 3.2, 14.4 Hz. 1H), 2.63 (dd, J = 10.4, 14.8 Hz, 1H),2.34-2.25 (m, 1H), 2.02- 1.77 (m, 6H), 1.65-1.60 (m, 2H), 1.52- 1.20 (m,15H), 1.18-1.00 (m, 6H), 0.94 (d, J = 6.8 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS: purity > 99%, analytic SFC: 99.28% de. MS ESI calcd. forC₂₆H₄₂NO₂S [M + H]⁺ 432.3, found 432.3. 430 H1 2- bromo- 5-methylthiazole

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.28 (s, 1H), 3.35-3.26 (m, 1H), 2.61-2.51(m, 1H), 2.42 (s, 3H), 2.00-1.76 (m, 7H), 1.68-1.58 (m, 3H), 1.49-1.32(m, 8H), 1.30-1.09 (m, 11H), 0.83 (d, J = 6.4 Hz, 3H), 0.77 (s, 3H).LC-ELSD/MS: purity > 99%, 100% de based on H- NMR. MS ESI calcd. forC₂₆H₄₂NOS [M + H]⁺ 416.3, found 416.3. 431

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.29 (s, 1H), 3.06 (dd, J = 3.2, 14.4 Hz,1H), 2.63-2.53 (m, 1H), 2.42 (s, 3H), 2.01- 1.77 (m, 7H), 1.50-1.32 (m,9H), 1.31- 1.05 (m, 13H), 0.94 (d, J = 6.4 Hz, 3H), 0.72 (s, 3H).LC-ELSD/MS: purity > 99%, analytic SFC: 100% de.MS ESI calcd. forC₂₆H₄₂NOS [M + H]⁺ 416.3, found 416.3. 432 H1 3- bromo- 5-methoxypyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.13 (d, J = 2.8 Hz, 1H), 8.02 (s, 1H),6.96 (s, 1H), 3.86 (s, 3H), 3.11 (dd, J = 4.0, 13.6 Hz, 1H), 2.12-1.98(m, 2H), 1.90-1.78 (m, 4H), 1.70-1.60 (m, 3H), 1.51-1.36 (m, 7H),1.32-1.23 (m, 9H), 1.13-1.02 (m, 5H), 0.80 (s, 3H), 0.70 (d, J = 6.4 Hz,3H). LC-ELSD/MS: purity > 99%, analytic SFC: 98.80% de. MS ESI calcd.for C₂₈H₄₄NO₂ [M + H]⁺ 426.3, found 426.3. 433

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.13 (d, J = 2.4 Hz, 1H), 8.02 (s, 1H),6.96 (s, 1H), 3.86 (s, 3H), 2.85 (dd, J = 2.8, 13.6 Hz, 1H), 2.14-1.92(m, 3H), 1.89-1.68 (m, 4H), 1.52-1.30 (m, 9H), 1.27-1.02 (m, 14H), 0.81(d, J = 6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS: purity > 99%, analyticSFC: 95.04% de. MS ESI calcd. for C₂₈H₄₄NO₂ [M + H]⁺ 426.3, found 426.3.434 H1 3- bromo- 5- methylpyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.25 (s, 1H), 8.19 (s, 1H), 2.83 (d, J =12.0 Hz, 1H), 2.31 (s, 3H), 2.09 − 1.92 (m, 3H), 1.88 −1.75 (m, 3H),1.65 − 1.59 (m, 3H), 1.51 − 1.32 (m, 8H), 1.32 − 1.00 (m, 14H), 0.80 (d,J = 6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS: purity > 99%, analytic SFC:99.84% de. MS ESI calcd. for C₂₈H₄₄NO [M + H]⁺ 410.3, found 410.3. 435H1 2- bromopyrimidine- 4- carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.88 (d, J = 4.8 Hz, 1H), 7.44 (d, J = 4.8Hz, 1H), 3.44 (dd, J = 4.0, 13.2 Hz, 1H), 2.63 (dd, J = 10.4, 13.2 Hz,1H), 2.20-2.10 (m, 1H), 2.02-1.95 (m, 1H), 1.90-1.77 (m, 4H), 1.68-1.59(m, 3H), 1.51-1.32 (m, 8H), 1.29-1.06 (m, 12H), 0.81 (s, 3H), 0.73 (d, J= 6.4 Hz, 3H). LC-ELSD/MS: purity > 99%, analytic SFC: 99.02% de. MS ESIcalcd. for C₂₇H₄₀N₃O [M + H]⁺ 422.3, found 422.3. 436

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.88 (d, J = 4.8 Hz, 1H), 7.44 (d, J = 4.4Hz, 1H), 3.18 (dd, J = 3.6, 13.2 Hz, 1H), 2.64 (dd, J = 10.4, 13.6 Hz,1H), 2.18-2.06 (m, 1H), 2.01-1.90 (m, 2H), 1.88-1.78 (m, 3H), 1.69-1.58(m, 3H), 1.51-1.34 (m, 8H), 1.33-1.08 (m, 12H), 0.85 (d, J = 6.4 Hz,3H), 0.73 (s, 3H). LC-ELSD/MS: purity > 99%, analytic SFC: 100% de. MSESI calcd. for C₂₇H₃₇N₃ [M − H₂O + H]⁺ 404.3, found 404.3. 437 H1 2-bromo- 5- methylpyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.49 (s, 2H), 3.35 (dd, J = 4.0, 12.8 Hz,1H), 2.51 (dd, J = 10.8, 13.2 Hz, 1H), 2.27 (s, 3H), 2.18-2.06 (m, 1H),2.05-1.97 (m, 1H), 1.91-1.77 (m, 4H), 1.91-1.77 (m, 4H), 1.50-1.31 (m,8H), 1.30-1.07 (m, 11H), 0.81 (s, 3H), 0.72 (d, J = 7.8 Hz, 3H).LC-ELSD/MS: purity > 99%, analytic SFC: 98.90% de. MS ESI calcd. forC₂₇H₄₃N₂O [M + H]⁺ 411.3, found 411.3. 438 H1 2- bromo- 5-methylpyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.49 (s, 2H), 3.16-3.07 (m, 1H), 2.50 (dd,J = 10.8, 13.2 Hz, 1H), 2.27 (s, 3H), 2.14-2.02 (m, 1H), 2.01-1.89 (m,2H), 1.88-1.77 (m, 3H), 1.67-1.60 (m, 2H), 1.50-1.31 (m, 8H), 1.30-1.02(m, 13H), 0.84 (d, J = 6.4 Hz, 3H), 0.73 (s, 3H). LC-ELSD/MS: purity >99%, analytic SFC: 100% de. MS ESI calcd. for C₂₇H₄₃N₂O [M + H]⁺ 411.3,found 411.3. 439 H1 2- bromo- 5- methylpyrazine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.38 (s, 1H), 8.27 (d, J = 1.2 Hz, 1H),3.21 (dd, J = 4.0, 13.2 Hz, 1H), 2.52 (s, 3H), 2.31 (dd, J = 10.8, 13.6Hz, 1H), 2.05-1.74 (m, 7H), 1.68-1.58 (m, 3H), 1.46-1.36 (m, 6H),1.32-1.24 (m, 8H), 1.12-1.02 (m, 5H), 0.81 (s, 3H), 0.70 (d, J = 6.4 Hz,3H). LC-ELSD/MS: purity > 99%, analytic SFC: 100% de. MS ESI calcd. forC₂₇H₄₃N₂O [M + H]⁺ 411.4 found 411.4 440

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.38 (s, 1H), 8.27 (d, J = 0.8 Hz, 1H),2.96 (dd, J = 3.2, 13.2 Hz, 1H), 2.52 (s, 3H), 2.31 (dd, J = 10.4, 13.6Hz, 1H), 2.04-1.77 (m, 6H), 1.67-1.62 (m. 2H), 1.51-1.30 (m, 8H),1.28-0.97 (m. 13H), 0.82 (d, J = 6.4 Hz, 3H), 0.71 (s, 3H), LC- ELSD/MS:purity > 99%, analytic SFC: 100% de. MS ESI calcd. for C₂₇H₄₃N₂O [M +H]⁺ 411.3 found 411.3 441 B1 2-bromo-6- methylpyrazine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.24 (s, 1H), 8.20 (s, 1H), 3.26-3.18 (m,1H), 2.53 (s, 3H), 2.37-2.27 (m, 1H), 2.07- 1.73 (m, 7H) 1.48-1.34 (m,7H), 1.30- 1.20 (m, 9H), 1.14-1.00 (m, 6H), 0.81 (s, 3H), 0.71 (d, J =6.4 Hz, 3H). LC- ELSD/MS: purity > 99%, analytic SFC: 98.22% de. MS ESIcalcd. for C₂₇H₄₃N₂O [M + H]⁺ 411.3 found 411.3 442

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.24 (s, 1H), 8.20 (s, 1H), 2.98 (dd, J =3.2, 13.2 Hz, 1H), 2.53 (s, 3H), 2.31 (dd, J = 10.4, 13.6 Hz 1H) 2.02 −1.77 (m, 7H) 1.50 − 1.33 (m, 8H), 1.31 − 1.19 (m, 8H), 1.17 − 1.00 (m,6H), 0.82 (d, J = 6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS: purity > 99%,analytic SFC: 100% de. MS ESI calcd. for C₂₇H₄₃N₂O [M + H]⁺ 411.3found411.3 443 H1 5- bromo- 2- (trifluoromethyl) pyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.64-7.54 (m, 2H), 3.23-3.16(m, 1H), 2.22-2.15 (m, 1H), 2.06-1.95 (m, 1H), 1.89-1.59 (m, 8H),1.51-1.34 (m, 7H), 1.33-1.07 (m, 13H), 0.80 (s, 3H), 0.70 (d, J = 6.8Hz, 3H). LC-ELSD/MS: purity > 99%, analytic SFC: 99.26% de. MS ESIcalcd. for C₂₈H₃₉F₃N [M − H₂O + H]⁺ 446.3 found 446.3 444

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.50 (s, 1H), 7.68-7.55 (m, 2H), 2.97-2.88(m, 1H), 2.26-2.16 (m, 1H), 2.08-1.59 (m, 9H), 1.51-1.33 (m, 7H),1.32-0.98 (m, 13H), 0.81 (d, J = 6.4 Hz, 3H), 0.70 (s, 3H). LC-ELSD/MS:purity > 99%, analytic SFC: 98.24% de. MS ESI calcd. for C₂₈H₃₉F₃N [M −H₂O + H]⁺ 446.3 found 446.3 445 305.2 5- bromopyrimidine- 4-carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.71 (d, J = 2.0 Hz, 1H), 8.59 (d, J = 1.8Hz, 1H), 7.72 (s, 1H), 3.16 (dd, J = 3.8, 13.8 Hz, 1H), 2.16 (dd, J =11.0, 13.6 Hz, 1H), 2.03-1.67 (m, 7H), 1.51-1.31 (m, 10H), 1.26 (s, 3H),1.24-1.04 (m, 8H), 0.96 (s, 3H), 0.78 (s, 3H), 0.70 (d, J = 6.4 Hz, 3H).LC-ELSD/MS: purity 99%, analytic SFC: 92.6% de; MS ESI calcd. forC₂₉H₄₁N₂ [M − H₂O + H]⁺ 417.3, found 417.3. 446

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.72 (d, J = 1.8 Hz, 1H), 8.59 (d, J = 2.0Hz, 1H), 7.72 (t, J = 1.8 Hz, 1H), 2.96-2.86 (m, 1H), 2.24-2.13 (m, 1H),2.06-1.61 (m, 8H), 1.54-1.37 (m, 9H), 1.26 (s, 3H), 1.22-0.99 (m, 8H),0.94 (s, 3H), 0.80 (d, J = 6.4 Hz, 3H), 0.69 (s, 3H). LC- ELSD/MS:purity 99%, analytic SFC: 100% de; MS ESI calcd. for C₂₉H₄₁N₂ [M − H₂O +H]⁺ 417.3, found 417.3; MS ESI calcd. for C₂₉H₄₃N₂O [M + H]⁺ 435.3,found 435.3. 447 323.1 5- bromo- 2- methylpyridine

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.27 (d, J = 2.0 Hz, 1H), 7.35 (br d, J =7.6 Hz, 1H), 7.06 (d, J = 8.0 Hz, 1H), 3.14-3.01 (m, 1H), 2.53 (s, 3H),2.11-1.99 (m, 2H), 1.91-1.62 (m, 8H), 1.49-1.17 (m, 16H), 1.14-0.99 (m,5H), 0.93 (t, J = 7.2 Hz, 3H), 0.79 (s, 3H), 0.69 (d, J = 6.4 Hz, 3H).LC-ELSD/MS purity 99%, MS ESI calcd for C₃₀H₄₈NO [M + H]⁺ 438.4, found438.4. SFC 95% de. 448

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.29 (d, J = 2.0 Hz, 1H), 7.37 (dd, J =2.0, 7.6 Hz, 1H), 7.08 (d, J = 8.0 Hz, 1H), 2.91- 2.78 (m, 1H), 2.54 (s,3H), 2.09 (dd, J = 10.2, 13.6 Hz, 1H), 2.06-1.94 (m, 2H), 1.88-1.62 (m,7H), 1.55-1.28 (m, 14H), 1.26-1.01 (m, 8H), 0.96 (t, J = 7.2 Hz, 3H),0.82 (d, J = 6.4 Hz, 3H), 0.71 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd for C₃₀H₄₈NO [M + H]⁺ 438.4, found 438.4. SFC 100% de. 449 2-bromo- 4- (trifluoromethyl)- 1, 3- thiazole

¹H NMR(400 MHz, CDCl₃) δ_(H) 7.62 (s, 1H), 3.39 (dd, J = 3.6, 14.4 Hz,1H), 2.73 (dd, J = 10.8, 14.4 Hz, 1H), 2.03-1.79 (m, 6H), 1.69-1.59 (m,3H), 1.45-1.25 (m, 14H), 1.17-1.00 (m, 6H), 0.84 (d, J = 6.4 Hz, 3H),0.78 (s, 3H).LC- ELSD/MS: purity > 99%, analytic SFC: 100% de, MS ESIcalcd. for C₂₆H₃₉F₃NOS [M + H]⁺ 470.3, found 470.3. 450

¹H NMR(400 MHz, CDCl₃) δ_(H) 7.62 (s, 1H), 3.16 (dd, J = 3.6, 14.4 Hz,1H), 2.71 (dd, J = 10.4, 14.8 Hz, 1H), 1.99-1.78 (m, 6H), 1.66-1.58 (m,3H), 1.50-1.33 (m, 8H), 1.29-1.07 (m, 12H), 0.95 (d, J = 6.8 Hz, 3H),0.72 (s, 3H)LC- ELSD/MS: purity > 99%, analytic SFC: 100% de, MS ESIcalcd. for C₂₆H₃₉F₃NOS [M + H]⁺ 470.2, found 470.2. 451 B1 5-iodo- 3-methyl- 1, 2- thiazole

¹H NMR (400 MHz, CDCl₃) δ_(H) 6.72 (s, 1H), 3.02-2.94 (m, 1H), 2.56 (dd,J = 9.4, 14.7 Hz, 1H), 2.45 (s, 3H), 2.00-1.63 (m, 8H), 1.49-1.32 (m,8H), 1.26 (s, 5H), 1.19-1.02 (m, 7H), 0.93 (d, J = 6.8 Hz, 3H), 0.70 (s,3H). LC-ELSD/MS: purity 99%, analytic SFC: 98.2% de; MS ESI calcd. forC₂₆H₄₂NOS [M + H]⁺ 416.3, found 416.3. 452

LC-ELSD/MS: analytic SFC: 98.2% de; MS ESI calcd. for C₂₆H₄₂NOS [M + H]⁺416.3, found 416.3. 453 305.2 2- bromo- 4- (trifluoromethyl)- 1,3-thiazole

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.62 (s, 1H), 3.43-3.34 (m, 1H), 2.77-2.68(m, 1H) 1.99-1.63 (m, 7H), 1.46-1.33 (m, 9H), 1.27-1.07 (m, 12H), 0.95(s, 3H), 0.84 (d, J = 6.4 Hz, 3H), 0.76 (s, 3H). LC-ELSD/MS: purity 99%,analytic SFC: 94.3% de; MS ESI calcd. for C₂₇H₄₁F₃NOS [M + H]⁺ 484.3,found 484.3. 454

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.62 (s, 1H), 3.21-3.12 (m, 1H), 2.77-2.65(m, 1H), 1.98-1.61 (m, 7H), 1.47-1.34 (m, 9H), 1.26-1.07 (m, 12H),0.96-0.93 (m, 6H), 0.70 (s, 3H). LC-ELSD/MS: purity 99%, analytic SFC:99.9% de; MS ESI calcd. for C₂₇H₄₁F₃NOS [M + H]⁺ 484.3, found 484.3. 455B1 ethyl 2- chlorooxazole- 5- carboxylate

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.65 (s, 1H), 4.37 (q, J = 7.1 Hz, 2H),3.17 (dd, J = 3.9, 14.7 Hz, 1H), 2.56 (dd, J = 10.5, 14.8 Hz, 1H),2.05-1.76 (m, 8H), 1.56-1.30 (m, 8H), 1.30-1.15 (m, 8H), 1.11-0.98 (m,7H), 0.84 (d, J = 6.5 Hz, 3H), 0.76 (s, 3H), 0.65 (s, 1H). LC- ELSD/MSpurity 99%, MS ESI calcd. for C₂₈H₄₄NO₄ [M + H]⁺ 458.3 found 458.3. SFC:99% de. 456

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.65 (s, 1H), 4.38 (q, J = 7.2 Hz, 2H),2.93 (dd, J = 3.6, 14.7 Hz, 1H), 2.55 (dd, J = 9.8, 14.8 Hz, 1H),2.17-1.74 (m, 6H), 1.62 (br d, J = 10.8 Hz, 2H), 1.49-1.25 (m, 17H),1.21-0.99 (m, 7H), 0.95 (d, J = 6.8 Hz, 3H), 0.75-0.67 (m, 1H), 0.71 (s,2H). LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₈H₄₄NO₄ [M + H]⁺ 458.3found 458.3. 457 323.1 4- bromobenzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.55 (d, J = 8.0 Hz, 2H), 7.23 (d, J = 8.0Hz, 2H), 2.93 (dd, J = 3.2, 13.3 Hz, 1H), 2.18-2.11 (m, 1H), 2.03-1.93(m, 2H), 1.82-1.60 (m, 7H), 1.58-1.43 (m, 6H), 1.39-1.27 (m, 8H),1.20-1.00 (m, 7H), 0.93 (t, J = 7.2 Hz, 3H), 0.78 (d, J = 6.4 Hz, 3H),0.69 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd for C₃₁H₄₄N [M − H₂O +H]⁺ 430.4, found 430.4. SFC 100% de. 458

LC-ELSD/MS MS ESI calcd for C₃₁H₄₄N [M − H₂O + H]⁺ 430.4, found 430.4.SFC 100% de. 459 323.1 4- bromo- 3- methylbenzonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.42- 7.38 (m, 2H), 7.16 (d, J = 7.6 Hz,1H), 2.91 (dd, J = 3.2, 13.4 Hz, 1H), 2.33 (s, 3H), 2.19-2.12 (m, 1H),2.05-1.95 (m, 2H), 1.83-1.60 (m, 7H), 1.50-1.26 (m, 13H), 1.26-0.98 (m,8H), 0.94 (t, J = 7.2 Hz, 3H), 0.77 (d, J = 6.4 Hz. 3H), 0.69 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd for C₃₂H₄₆N [M − H₂O + H]⁺ 444.4,found 444.4. SFC 100% de. 460

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.42- 7.37 (m, 2H), 7.16 (d, J = 7.6 Hz,1H), 3.10 (dd, J = 4.4, 13.6 Hz, 1H), 2.33 (s, 3H), 2.22 (dd, J = 11.2,13.2 Hz, 1H), 2.03-1.97 (m, 1H), 1.87-1.60 (m, 8H), 1.52-1.44 (m, 3H),1.41-1.19 (m, 13H), 1.13-1.02 (m, 5H), 0.94 (t, J = 7.2 Hz, 3H), 0.79(s, 3H), 0.66 (d, J = 6.4 Hz. 3H). LC-ELSD/MS purity 99%, MS ESI calcdfor C₃₂H₄₆N [M − H₂O + H]⁺ 444.4. found 444.4. SFC 99% de.

Examples 487 & 488: Synthesis of4-(((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2,2,2-trifluoroethyl)amino)-3-methylbenzonitrile(487)&4-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2,2,2-trifluoroethyl)amino)-3-methylbenzonitrile(488)

Synthesis of 487.1

To a solution of 357.1 (1.5 g, 4.30 mmol) in anhydrous THF (30 mL) wasadded CsF (1.62 g, 10.7 mmol) at 0° C., After stirring for 20 mins,TMSCF₃ (1.51 g, 10.7 mmol) was added at 0° C. After stirring for 1 h,TBAF.3H₂O (5.43 g, 17.2 mmol) was added. After stirring at 50° C. foranother 1 h, the reaction mixture was poured into ice-water (50 mL),stirred for 10 mins. The and extracted with EtOAc (2×30 mL). Thecombined organic phase was washed with brine (2×20 mL), dried overanhydrous Na₂SO₄, filtered and concentrated in vacuum to give 487.1 (1.7g) as a solid.

Synthesis of 487.2

To a solution of 487.1 (1.7 g, 4.06 mmol) in DCM (50 mL) was added DMP(4.28 g, 10.1 mmol) at 25° C. After stirring at 35° C. for 1 h, thereaction mixture was quenched with saturated NaHCO₃/Na₂S₂O₃ (50 mL/50mL). The organic was separated and the aqueous was extracted with DCM(2×30 mL). The combined organic layer was washed with aqueous saturatedNaHCO₃/Na₂S₂O₃ (50 mL/50 mL), brine (30 mL), dried over anhydrousNa₂SO₄, filtered and concentrated to give 487.2 (1.25 g) as a solid.

¹H NMR (400 MHz, CDCl3) δ 3.55-3.48 (m, 2H), 3.48-3.30 (m, 2H),3.30-2.90 (m, 1H), 2.72 (s, 1H), 2.20-2.05 (m, 1H), 1.90-1.50 (m, 8H),1.50-1.25 (m, 10H), 1.25-0.85 (m, 7H), 0.72 (m, 3H). ¹⁹F NMR (376 MHz,CDCl₃) δ −78.68. LC-ELSD/MS 99%, MS ESI calcd. for C₂₃H₃₅F3O₃Na[M+Na]⁺439, found 439.

Synthesis of 487.3

To a solution of 487.2 (600 mg, 1.44 mmol) and CH₃CH₂ONa (234 mg, 3.45mmol) in EtOH (10 mL) was added hydroxylamine hydrochloride (200 mg,2.88 mmol) at 25° C. After stirring at 25° C. for 16 h, the mixture wasdiluted with water (100 mL) and extracted with EtOAc (3×100 mL). Thecombined organic solution was dried over anhydrous Na₂SO₄, filtered andconcentrated to give 487.3 (0.58 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.56-3.49 (m, 2H), 3.47-3.35 (m, 3H),2.62-2.57 (m, 2H), 2.19-2.08 (m, 2H), 1.85-1.32 (m, 9H), 1.26-1.15 (m,7H), 1.12-0.75 (m, 8H), 0.65 (s, 3H).

Synthesis of 487.4

To a mixture of 487.3 (480 mg, 1.11 mmol) in THF (10 mL) was addedLiAlH₄ (252 mg, 6.66 mmol) at 0° C. After stirred at reflux for 6 h, themixture was diluted with water (100 mL) and NaOH (15%, 100 mL) andextracted with EtOAc (3×100 mL). The combined organic solution was driedover anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by column chromatography on silica gel (PE:EtOAc=4:1) to give487.4 (300 mg) as an oil.

¹H NMR (400 MHz, CDCl3) δ_(H) 3.56-3.48 (m, 2H), 3.47-3.37 (m, 2H),3.26-3.17 (m, 1H), 2.90-2.47 (m, 1H), 1.86-1.55 (m, 11H), 1.49-1.33 (m,6H), 1.28-1.23 (m, 2H), 1.20 (t, J=7.2 Hz, 4H), 1.15-1.09 (m, 2H), 1.06(d, J=8.8 Hz, 3H), 0.90-0.80 (m, 1H), 0.72 (s, 3H). ¹⁹F NMR (376.5 MHz,CDCl₃) δ_(F) −77.258.

Synthesis of 487.5

To a solution of 487.4 (150 mg, 0.359 mmol), 4-bromobenzonitrile (105mg, 0.538 mmol), BINAP (44.6 mg, 0.0718 mmol) and Cs₂CO₃ (348 mg, 1.07mmol) in toluene (6 mL) was added Pd(OAc)₂ (16.1 mg, 0.0718 mmol) at 25°C. under N₂. After stirring at 110° C. for 16 h, the resultingsuspension was filtered through a pad of Celite and washed with EtOAc(3×20 mL). The filtrate was concentrated. The residue was purified byflash column (0˜25% of EtOAc in PE) to give 487.5 (120 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.43-7.40 (m, 1H), 7.34-7.32 (m, 1H), 6.66(d, J=8.4 Hz, 1H), 4.03-3.93 (m, 1H), 3.92-3.86 (m, 1H), 3.52 (q, J=7.2Hz, 2H), 3.41 (q, J=9.2 Hz, 2H), 2.73 (s, 1H), 2.17-2.15 (m, 3H),1.98-1.89 (m, 1H), 1.81-1.72 (m, 6H), 1.66-1.58 (m, 3H), 1.51-1.45 (m,1H), 1.43 (s, 3H), 1.36 (d, J=9.6 Hz, 4H), 1.20 (t, J=7.2 Hz, 4H),1.17-0.87 (m, 5H), 0.63 (s, 3H). ¹⁹F NMR (376.5 MHz, CDCl3) δ_(F)−72.898.

Separation 487 & 488

487.5 (120 mg, 0.225 mmol) was separated by SFC (Column: DAICELCHIRALPAK AD-H 250×30 mm I.D., Sum; Condition: 0.1% NH₃H₂O ETOH;Gradient: from 35% to 35% of B; Flow rate: 50 mL/min; Columntemperature: 40° C.) to afford 487 (56.3 mg, 47%, Peak 1, Rt=3.648 min)as a solid and 488 (10 mg, Peak 2, Rt=5.086 min) as an oil.

487: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.44-7.39 (m, 1H), 7.35-7.31 (m, 1H),6.68-6.63 (m, 1H), 4.04-3.94 (m, 1H), 3.92-3.85 (m, 1H), 3.57-3.48 (m,2H), 3.46-3.36 (m, 2H), 2.72 (s, 1H), 2.15 (s, 3H), 2.01-1.88 (m, 1H),1.81-1.57 (m, 10H), 1.50-1.33 (m, 6H), 1.31-1.23 (m, 2H), 1.20 (t, J=7.2Hz, 4H), 1.16-0.90 (m, 4H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₃₁H₄₂F3N₂O [M−H₂O+H]⁺415.3 found 415.3. SFC 100% de. ¹⁹F NMR(376.5 MHz, CDCl₃) δ_(F) −72.900.

488: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.46-7.41 (m, 1H), 7.35-7.31 (m, 1H),6.69 (d, J=8.4 Hz, 1H), 4.14-4.01 (m, 2H), 3.56-3.49 (m, 2H), 3.46-3.38(m, 2H), 2.71 (s, 1H), 2.17 (s, 3H), 1.96-1.87 (m, 2H), 1.84-1.74 (m,5H), 1.73-1.66 (m, 1H), 1.64-1.57 (m, 3H), 1.50-1.35 (m, 6H), 1.20 (t,J=7.2 Hz, 8H), 1.07-0.96 (m, 2H), 0.52 (s, 3H). LC-ELSD/MS purity 99%,MS ESI calcd. for C₃₁H₄₂F3N₂O [M−H₂O+H]⁺415.3 found 415.3. ¹⁹F NMR(376.5 MHz, CDCl₃) δ_(F), −74.694.

Examples 489 & 490: Synthesis of3-methyl-4-(((S)-2,2,2-trifluoro-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)amino)benzonitrile(489) &3-methyl-4-(((R)-2,2,2-trifluoro-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)amino)benzonitrile(490)

Synthesis of 489.1

To a solution of A2 (9.5 g, 31.2 mmol), CsF (4.73 g, 31.2 mmol) in THF(300 mL) was added TMSCF₃ (13.2 g, 93.5 mmol) at 0° C. under N₂. Afterstirring at 0° C. for 1 h, TBAF (40.7 g, 156 mmol) was added. Afterstirring at 20° C. for 16 h, the mixture was poured into water (50 mL)and concentrated to remove THF. The residue was extracted with EtOAc(2×400 mL). The combined organic phase was washed with brine (250 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (0% 9% of EtOAc in PE) to give 489.1 (4.0 g,94%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.02 (br d, J=7.2 Hz, 1H), 2.09-1.83 (m,4H), 1.78-1.65 (m, 8H), 1.51-1.34 (m, 8H), 1.26 (s, 3H), 1.14-0.92 (m,6H), 0.73 (s, 3H).

Synthesis of 489.2

To a solution of 489.1 (500 mg, 1.33 mmol) in DCM (20 mL) at 0° C. wasadded DMP (1.40 g, 3.32 mmol). After stirring at 25° C. for 16 h, themixture was poured into brine (15 mL) and extracted with EtOAc (30mL×3). The combined organic phase was dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was triturated from CH₃CN (20 mL)at 25° C. to give 489.2 (500 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.95 (s, 1H), 2.62-2.49 (m, 1H), 2.04 (s,2H), 1.80 (m, 6H), 1.51-1.20 (m, 13H), 1.09 (br s, 4H), 0.88 (s, 1H),0.75-0.68 (m, 3H).

Synthesis of 489.3

To a solution of 489.2 (1 g, 2.68 mmol) and EtONa (558 mg, 8.04 mmol) inEtOH (30 mL) was added hydroxylamine hydrochloride (372 mg, 5.36 mmol)at 25° C. After stirring at 40° C. for 24 h, the mixture was dilutedwith water (100 mL) and extracted with EtOAc (3×100 mL). The combinedorganic solution was dried over anhydrous Na₂SO₄, filtered andconcentrated to give 489.3 (900 mg, 87%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.80-3.60 (m, 1H), 2.22-1.63 (m, 17H),1.53-0.96 (m, 10H), 0.81-0.57 (m, 4H).

Synthesis of 489.4

To a mixture of 489.3 (900 mg, 2.32 mmol) in THF (10 mL) was addedLiAlH₄ (527 mg, 13.9 mmol) at 25° C. After stirring at 80° C. for 6 h,the reaction was combined with another batch prepared from 300 mg of489.3 to work-up. The mixture was diluted with water (20 mL) and EtOAc(50 mL). The mixture was then treated with NaOH (20 mL, 15%) and water(50 mL). The resulting mixture was filtered and concentrated. Theresidue was washed with HCl (20 mL, 1 M) and EtOAc (20 mL). The aqueousphase was adjusted with NaOH (30 mL, 2 M) to pH 13 and extracted withEtOAc (2×50 mL). The combined organic layer was concentrated. Theresidue was purified by flash column (0˜ 15% of EtOAc in PE) to give489.4 (240 mg, 30%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.94-3.81 (m, 1H), 3.29-3.00 (m, 1H), 2.23(td, J=3.2, 12.4 Hz, 1H), 2.10 (td, J=3.2, 12.8 Hz, 1H), 1.90-1.75 (m,10H), 1.68-1.58 (m, 8H), 1.44-1.37 (m, 8H), 1.10-1.04 (m, 8H), 0.78 (d,J=4.4 Hz, 4H), 0.73 (s, 2H).

Synthesis of 489 & 490

To a solution of 489.4 (180 mg, 0.482 mmol),4-bromo-3-methylbenzonitrile (141 mg, 0.723 mmol), BINAP (59.9 mg,0.0964 mmol) and (Cs₂CO₃ 469 mg, 1.44 mmol) in toluene (2 mL) was addedPd(OAc)₂ (21.6 mg, 0.09638 mmol) at 25° C. under N₂. After stirring at110° C. for 16 h, the mixture was poured into water (20 mL) andextracted with EtOAc (2×50 mL). The combined organic phase was washedwith brine (20 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜30% of EtOAcin PE) to give 490 (59 mg) and 489 (40 mg) both as solids. 489 (39.9 mg,0.0816 mmol) was further purified by SFC (Column DAICEL CHIRALCEL OD-H(250 mm*30 mm, 5 um); Condition 0.1% NH₃H₂O ETOH; FlowRate(ml/min): 50)to give 489 (20.7 mg, 52.0%) as a solid. 490 (59 mg, 0.120 mmol) wasfurther purified by SFC (Column DAICEL CHIRALCEL OJ-H (250 mm*30 mm, 5um); Condition Neu-ETOH; FlowRate(ml/min) δ0) to give 490 (19.7 mg,33.6%) as a solid.

489: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.42 (d, J=8.4 Hz, 1H), 7.33 (s, 1H),6.66 (d, J=8.8 Hz, 1H), 4.06-3.94 (m, 1H), 3.88 (br d, J=9.6 Hz, 1H),3.72 (br d, J=5.2 Hz, 1H), 2.15 (s, 3H), 1.94 (br s, 1H), 1.81-1.72 (m,5H), 1.61 (br d, J=13.6 Hz, 3H), 1.51-1.34 (m, 6H), 1.32-1.18 (m, 9H),1.13-0.90 (m, 3H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd forC₂₉H₃₈F3N₂[M−H₂O+H]⁺471, found 471; C₂₉H₄₀F3N₂O [M+H]⁺ 489, found 489;C₂₉H₃₉F3N₂ONa[M+H]⁺ 511, found 511. ¹⁹F NMR (376.5 MHz, CDCl₃) δ_(F)−72.917.

490: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.49-7.38 (m, 1H), 7.33 (s, 1H), 6.69(d, J=8.4 Hz, 1H), 4.18-4.00 (m, 2H), 2.17 (s, 3H), 1.98-1.74 (m, 7H),1.73-1.58 (m, 3H), 1.51-1.29 (m, 8H), 1.26 (s, 4H), 1.23-0.94 (m, 6H),0.52 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd forC₂₉H₃₈F3N₂[M−H₂O+H]⁺471, found 471; C₂₉H₄₀F3N₂O [M+H]⁺ 489, found 489;C₂₉H₃₉F3N₂ONa[M+H]⁺ 511, found 511. ¹⁹F NMR (376.5 MHz, CDCl₃) δ_(F)−74.722.

Example 491: Synthesis of5-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-ethyl-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethyl)amino)picolinonitrile(491)

Synthesis of 491.1

To a solution of BHT (9.2 g, 41.7 mmol) in toluene (60 mL) under N₂ at0° C. was added trimethylaluminum (2 M in toluene, 10.4 mL, 20.8 mmol)dropwise. After stirring at 25° C. for 1 h, a solution of 271.3 (2.1 g,6.94 mmol) in toluene (10 mL) at −70° C. was added dropwise to the abovesolution. After stirring at −70° C. for 1 h under N₂, EtMgBr (6.93 mL,20.8 mmol, 3 M in ethyl ether) was added dropwise at −70° C. Afterstirring at −70° C. for 1 h, the reaction mixture was poured intoaqueous citric acid (30 mL, sat.) at 10° C. and extracted with DCM (2×30mL). The combined organic layer was dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column (0˜30% of EtOAc in PE) to give 491.1 (1.76 g, 76%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 2.58-2.55 (m, 1H), 2.19-2.15 (m, 4H),2.13-2.00 (m, 1H), 1.78-1.60 (m, 10H), 1.59-1.51 (m, 8H), 1.50-1.25 (m,3H), 1.24-1.00 (m, 3H), 0.91 (t, J=7.6, 3H), 0.63 (s, 3H).

Synthesis of 491.2

To a solution of 491.1 (1.76 g, 5.29 mmol) in DCE (15 mL) with(1R)-1-phenylethan-1-amine (3.84 g, 31.7 mmol) at 25° C. was addedNaCNBH₃ (2.65 g, 42.3 mmol) at 25° C. After stirring at 50° C. for 16 h,the reaction mixture was quenched with water (15 mL) and extracted withDCM (2×15 mL). The combined organic phase was washed with brine (15 mL),dried over anhydrous Na₂SO₄, filtered, and concentrated. The residue waspurified by flash column (0˜ 30% of EtOAc in PE) to give 491.2 (2.54 g)as a solid, which was re-purified by flash column (0˜ 30% of EtOAc inPE) to give 491.2 (2 g, 79%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.27-7.15 (m, 5H), 4.08-4.01 (m, 1H),4.00-3.60 (m, 1H), 2.45-2.43 (m, 1H), 2.42-2.17 (m, 1H), 2.00-1.98 (m,2H), 1.97-1.96 (m, 2H), 1.95-1.75 (m, 2H), 1.75-1.48 (m, 6H), 1.47-1.18(m, 13H), 1.17-0.96 (m, 6H), 0.95-0.81 (m, 4H), 0.80-0.70 (m, 3H).

Synthesis of 491.3

To a solution of 491.2 (1 g, 2.28 mmol) in EtOH (10 mL) was added dryPd—C (800 mg, 10%) under N₂. The suspension was degassed under vacuumand purged with H₂ for three times. After stirring under H₂ (50 psi) at50° C. for 48 h, the resulting suspension was filtered through a pad ofCelite and washed with THF (3×40 mL). The filtrate was concentrated. Theresidue was purified by flash column (5˜ 100% of EtOAc in PE) to give491.3 (308 mg, 31%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.75-3.70 (m, 1H), 2.05-2.04 (m, 1H),1.76-1.72 (m, 1H), 1.71-1.51 (m, 8H), 1.50-1.21 (m, 12H), 1.20-1.00 (m,10H), 0.88 (t, J₁=7.2, J₂=7.6, 3H), 0.75 (s, 3H).

Synthesis of 491

To a mixture of 491.3 (300 mg, 0.899 mmol),5-bromopyridine-2-carbonitrile (327 mg, 1.79 mmol), BINAP (55.9 mg,0.0897 mmol) and Cs₂CO₃ (583 mg, 1.79 mmol) in toluene (5 mL) was addedPd(OAc)₂ (20.1 mg, 0.0895 mmol) at 25° C. under N₂. After stirring at120° C. for 18 h, the resulting suspension was diluted with water (10mL) and extracted with EtOAc (2×10 mL). The combined organic layers wasconcentrated and purified twice by flash column (0˜ 20% of EtOAc in PE)to give 491 (150 mg) as a solid, which was further purified bypre-HPLC(Column: Xtimate C18 150*25 mm*5 um), Condition: water (10 mMNH₄HCO₃)-ACN, Begin B: 65%, End B: 95%, Gradient Time(min): 9.5, 100% BHold Time(min): 2, Flow Rate (ml/min): 25) to afford 491 (66.2 mg, 74%)as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.94 (d, J=2.4, 1H), 7.44 (d, J=8.4, 1H),6.77-6.73 (m, 1H), 4.15-4.00 (m, 1H), 3.50-3.83 (m, 1H), 1.95-1.80 (m,2H), 1.79-1.52 (m, 8H), 1.51-1.20 (m, 12H), 1.18-0.91 (m, 8H), 0.88 (t,J=7.2, 3H), 0.62 (s, 3H). LC-ELSD/MS purity 99%; MS ESI calcd. forC₂₈H₄₂N₃O [M+H]⁺ 436, found 436.

Example 492: Synthesis of3-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)amino)benzonitrile(492)

Synthesis of 492.1

To a solution of A27 (9, 25.6 mmol) in DCM (100 mL) was added PCC (11.0g, 51.2 mmol) and silica gel (15 g) at 25° C. After stirring at 25° C.for 2 h, the reaction mixture was filtered and the residue was washedwith DCM (2×100 mL). The combined filtrate was concentrated. The residuewas purified by flash column (0˜ 20% of EtOAc in PE) to give 492.1 (3.1g, 35%) as a solid.

¹H NMR (400 MHz, CDCl3) δ 9.72-9.66 (m, 1H), 3.50-3.42 (m, 2H),3.42-3.30 (m, 2H), 2.30-2.20 (m, 1H), 2.15-1.80 (m, 4H), 1.80-1.50 (m,2H), 1.50-1.25 (m, 8H), 1.25-1.00 (m, 12H), 0.90 (s, 1H), 0.75 (s, 3H).

Synthesis of 492.2

To a mixture of (R)-2-methylpropane-2-sulfinamide (2.07 g, 17.1 mmol),pyridinium toluene-4-sulfonate (179 mg, 0.715 mmol) and MgSO₄ (8.58 g,71.5 mmol) in DCE (200 mL) was added 492.1 (5 g, 14.3 mmol) at 25° C.After stirring at 50° C. for 16 h, the mixture was poured into water(100 mL) and extracted with EtOAc (2×150 mL). The combined organic phasewas washed with brine (100 mL), dried over anhydrous Na₂SO₄, filteredand concentrated. The residue was purified by flash column (0˜ 10% ofEtOAc in PE) to give 492.2 (2.54 g, 39%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.03 (d, J=5.6 Hz, 1H), 3.53 (q, J=6.8 Hz,2H), 3.42 (q, J=9.2 Hz, 2H), 2.55-2.45 (m, 1H), 2.05-1.50 (m, 14H),1.50-1.20 (m, 9H), 1.20-0.95 (m, 13H), 0.74 (s, 3H).

Synthesis of 492.3

To a solution of 492.2 (700 mg, 1.54 mmol) in THF (10 mL) was addedEtMgBr (1.53 mL, 3M, 4.62 mmol) at −78° C. under N₂. After stirring at−78° C. for 15 mins and warming to 25° C. for 30 mins, the reaction wasquenched by saturated NH₄Cl aq. (30 mL) and extracted by EtOAc (3×20mL). The combined organic phase was dried over Na₂SO₄ and concentratedto give 492.3 (703.7 mg). The product was used to next step directly.The stereochemistry at C20 was assigned based on the mechanism of Ellmanreaction.

Synthesis of 492.4

To a solution of 492.3 (703.7 mg, 1.45 mmol) in dioxane (7 mL) was addedHCl/dioxane (1.08 mL, 4M, 0.165 mL) at 25° C. After stirring at 25° C.for 2 h, the reaction mixture was combined with another batch preparedfrom 106 mg of 492.3, poured into saturated NaHCO₃. The resultingmixture was extracted with DCM (3×20 mL). The combined organic phase wasdried over anhydrous Na₂SO₄ and concentrated. The residue was purifiedby flash column (0˜ 20% MeOH in DCM) to give 492.4 (302.6 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.52 (q, J=7.2 Hz, 2H), 3.41 (q, J=9.2 Hz,2H), 2.76-2.65 (m, 1H), 2.25-1.50 (m, 13H), 1.50-1.10 (m, 14H),1.10-0.85 (m, 8H), 0.67 (s, 3H).

Synthesis of 492

A mixture of 492.4 (100 mg, 0.WXR-36 mmol), 3-bromobenzonitrile (96.1mg, 0.528 mmol), BINAP (49.2 mg, 0.079 mmol) and cesium carbonate (172mg, 0.528 mmol) in toluene (1.5 mL) was sparged with N₂ for 2 mins, thenPd(OAc)₂ (17.7 mg, 0.079 mmol) was added at 25° C. After stirring at110° C. for 16 h, the resulting suspension was combined with anotherbatch prepared from 30 mg of 492.4, filtered through a pad of Celite andwashed with EtOAc (3×10 mL). The filtrate was concentrated. The residuewas purified by flash column (0˜ 20% of EtOAc in PE) to give 492 (40 mg)as an oil. The 494 (40.0 mg, 0.835 mmol) was further purified by HPLC(Column Xtimate C18 150*25 mm*5 um; Condition water (0.225% FA)-ACNBegin B 95 End B 100 Gradient Time (min) 7 100% B Hold Time (min) 1FlowRate (ml/min) 25) to afford 492 (7.00 mg, 6%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.16 (br t, J=7.7 Hz, 1H), 6.85 (br d,J=7.4 Hz, 1H), 6.78-6.68 (m, 2H), 3.53 (q, J=6.9 Hz, 2H), 3.48-3.38 (m,2H), 3.33 (br s, 1H), 1.97-1.61 (m, 9H), 1.50-1.30 (m, 11H), 1.30-0.96(m, 11H), 0.86 (t, J=7.3 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%,MS ESI calcd. for C₃₁H₄₇N₂O₂ [M+H]⁺ 479.3 found 479.3.

Example 493: Synthesis of5-(((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)amino)picolinonitrile(493)

Synthesis of 493.2

To a mixture of bromotriphenylpropylphosphane (45.8 g, 119 mmol) in THF(200 mL) was added t-BuOK (13.3 g, 19 mmol) at 15° C. under N₂. Afterstirring at 50° C. for 6 h, 493.1 (10 g, 29.8 mmol) was added inportions at 50° C. After stirring at 50° C. for 16 h, the resultingsuspension was quenched with 10% NH₄Cl aqueous (400 mL) at 15° C. andextracted with EtOAc (300 mL). The combined organic phase wasconcentrated. The residue was purified by flash column (0˜ 10% of EtOAcin PE) to give 493.2 (6 g, 56%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 5.01 (t, J=7.2 Hz, 1H), 3.61-3.35 (m, 4H),2.68 (s, 1H), 2.44-2.28 (m, 1H), 2.26-1.96 (m, 4H), 1.88-1.67 (m, 5H),1.51-1.41 (m, 4H), 1.30-1.03 (m, 12H), 0.99-0.89 (m, 4H), 0.88-0.84 (m,4H).

Synthesis of 493.3

To a solution of 493.2 (6 g, 16.6 mmol) in THF (100 mL) was added 9-BBNdimmer (8.10 g, 33.2 mmol) at 15° C. After stirring at 60° C. for 6 h,the reaction mixture was cooled and sequentially treated with EtOH (20mL) at 0° C. and NaOH (49.8 mL, 5M, 249 mmol). After addition, H₂O₂(33.2 mL, 332 mmol, 10 M in water) was added slowly until the innertemperature did not increased any more and the inner temperature wasmaintained below 15° C. After stirring at 80° C. for another 2 h, theaqueous phase was extracted with ethyl acetate (3×100 mL). The combinedorganic phase was washed with saturated Na₂S₂O₃ (2×50 mL), brine (2×100mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (15% of ethyl acetate in PE) to give 493.3(5 g, 80%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.59-3.36 (m, 5H), 2.72-2.66 (m, 1H),1.90-1.70 (m, 5H), 1.67-1.52 (m, 7H), 1.49-1.30 (m, 8H), 1.28-1.02 (m,10H), 0.99-0.90 (m, 3H), 0.67 (s, 3H).

Synthesis of 493.4

To a solution of 493.3 (4 g, 10.5 mmol) in DCM (100 mL) was added DMP(8.90 g, 21.0 mmol) at 15° C. After stirring at 45° C. for 30 mins, theresulting colourless solution was poured into NaHCO₃.aq (50 mL) andextracted with EtOAc (3×30 mL). The combined organic solution was washedwith Na₂S₂O₃.aq (50 mL), brine (2×20 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜15% of EtOAc in PE) to give 493.4 (2.8 g, 71%) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.59-3.34 (m, 4H), 2.52 (t, J=8.8 Hz, 1H),2.43-2.32 (m, 2H), 2.21-2.10 (m, 1H), 1.94 (td, J=3.3, 12.3 Hz, 1H),1.86-1.54 (m, 8H), 1.52-1.34 (m, 7H), 1.30-1.14 (m, 7H), 1.08-0.98 (m,6H), 0.64-0.53 (m, 3H).

Synthesis of 493.5 & 493.5a

To a suspension 493.4 (3.2 g, 8.49 mmol) in toluene (50 mL) were addedTsOH (73.0 mg, 0.4245 mmol), 1-phenylmethanamine (4.54 g, 42.4 mmol),and Ti(OCH(CH₃)₂)₄ (12.0 g, 42.4 mmol). After stirring at 115° C. for 16h, the reaction was concentrated. To the residue (4.1 g, 8.80 mmol) inMeOH (50 mL) was added NaBH₄ (563 mg, 17.5 mmol) at 15° C. Afterstirring at 15° C. for 16 h, the resulting colorless solution was pouredinto water (400 mL) and extracted with EtOAc (3×400 mL). The combinedorganic phase was washed with brine (2×200 mL), dried over anhydrousNa₂SO₄, filtered and concentrated. The residue was purified by flashcolumn (0˜ 35% of EtOAc in PE) to give 493.5 (0.45 g) as an oil and493.5a (3.35 g) as an oil.

493.5: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.39-7.30 (m, 4H), 7.25-7.20 (m,1H), 3.83 (br d, J=11.2 Hz, 1H), 3.62 (br d, J=12.8 Hz, 1H), 3.53 (q,J=7.2 Hz, 2H), 3.46-3.38 (m, 2H), 2.79-2.52 (m, 2H), 1.96-1.72 (m, 6H),1.70-1.56 (m, 7H), 1.46-1.30 (m, 8H), 1.20 (t, J=7.2 Hz, 5H), 1.06-0.97(m, 4H), 0.93 (t, J=7.6 Hz, 3H), 0.69-0.62 (m, 3H).

494.5a: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.36-7.31 (m, 4H), 7.24-7.18 (m,1H), 3.82-3.77 (m, 1H), 3.60 (d, J=12.8 Hz, 1H), 3.57-3.49 (m, 2H),3.46-3.37 (m, 2H), 2.77-2.65 (m, 1H), 2.65-2.57 (m, 1H), 2.12 (br d,J=12.4 Hz, 1H), 1.87-1.54 (m, 12H), 1.50-1.32 (m, 10H), 1.22-0.96 (m,12H), 0.87 (t, J=7.6 Hz, 3H), 0.79 (s, 1H), 0.67 (s, 3H).

Synthesis of 493.6

To a solution of 493.5 (0.4 g, 0.855 mmol) in EtOH (10 mL) was addedPd/C (wet, 10%, 0.4 g) under N₂. The suspension was degassed undervacuum and purged with H₂ for three times. After stirring under H₂ (50psi) at 25° C. for 12 h, the resulting suspension was filtered through apad of Celite and washed with EtOH (3×15 mL). The filtrate wasconcentrated. The residue was purified by flash column (0˜ 10% of MeOHin DCM) to give 493.6 (322 mg) as a solid, which was purified by flashcolumn (0˜ 10% of MeOH in DCM) to give 493.6 (96 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.52 (q, J=6.8 Hz, 2H), 3.42 (q, J=9.2 Hz,2H), 2.73 (br s, 1H), 2.68-2.57 (m, 1H), 1.99-1.75 (m, 5H), 1.74 (br s,2H), 1.60 (br dd, J=13.2, 17.2 Hz, 5H), 1.51-1.31 (m, 8H), 1.28-0.99 (m,12H), 0.98-0.87 (m, 3H), 0.70-0.62 (m, 3H).

Synthesis of 493

To a solution of 5-bromopyridine-2-carbonitrile (139 mg, 0.762 mmol) intoluene (2 mL) were added 493.6 (96 mg, 0.254 mmol), Cs₂CO₃ (248 mg,0.762 mmol) and BINAP (31.5 mg, 0.0508 mmol) under N₂. After stirring at25° C. for 20 mins, Pd (OAc)₂ (11.4 mg, 0.0508 mmol) was added. Afterstirring at 110° C. for 16 h, the reaction was filtered andconcentrated. The residue was purified by flash column (0˜ 80% of EtOAcin PE) to give 493 (30 mg) as a solid, which was further purified by SFC(Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, Sum); Condition: 0.1%NH₃H₂O ETOH Begin B 35% End B 35%; FlowRate (ml/min): 60) to afford 493(4.3 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.98 (d, J=2.8 Hz, 1H), 7.41 (d, J=8.8 Hz,1H), 6.78 (dd, J=2.8, 8.5 Hz, 1H), 4.02 (br d, J=9.2 Hz, 1H), 3.53 (q,J=7.2 Hz, 2H), 3.42 (q, J=9.2 Hz, 3H), 2.72 (s, 1H), 1.93-1.73 (m, 6H),1.71-1.60 (m, 3H), 1.52-1.29 (m, 10H), 1.28-1.11 (m, 8H), 1.07 (br s,3H), 0.87 (t, J=7.3 Hz, 3H), 0.72 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd for C₃₀H₄₄N₃O[M−H₂O+H]⁺462, found 471; C₃₀H₄₆N₃O₂ [M+H]⁺ 480,found 480; C₃₀H₄₅N₃O₂Na[M+Na]⁺502, found 502.

Examples 494 & 495: Synthesis of5-(((S)-1-((3R,5R,8R,9S,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)amino)picolinonitrile(494) &5-(((R)-1-((3R,5R,8R,9S,10S,13S,14S,17S)-3-(ethoxymethyl)-3-hydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)amino)picolinonitrile(495)

Synthesis of 492.2

To a solution of NaH (4.95 g, 60%, 124 mmol) in DMSO (100 mL) and THF(100 mL) was added iodotrimethyl-sulfane (25.3 g, 124 mmol) at 0° C.over 30 mins under N₂, then a solution of 494.1 (30 g, 104 mmol) in DMSO(100 mL) was added. After stirring at 25° C. for another 3 h, theresulting suspension was poured into ice-water (500 mL), stirred for 20mins, and extracted with EtOAc (3×400 mL). The combined organic phasewas washed with brine (2×300 mL), dried over anhydrous Na₂SO₄, filteredand concentrated to give 494.2 (28 g) as an oil.

Synthesis of 494.3

Add Na (23.6 g, 594 mmol) to EtOH (500 mL) in batches at 25° C. underN₂. After stirred at 25° C. for 30 mins, 494.2 (30 g, 99.1 mmol) wasadded at 25° C. After stirring at 65° C. 16 h, the resulting solutionwas poured into water (500 mL) and extracted with EtOAc (3×400 mL). Thecombined organic phase was washed with brine (2×200 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜15% of EtOAc in PE) to give 494.3 (8.7 g, 25.2%) as anoil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.53 (q, J=6.9 Hz, 2H), 3.48-3.35 (m, 2H),2.70 (s, 1H), 2.43 (br dd, J=8.8, 19.1 Hz, 1H), 2.09 (br d, J=9.3 Hz,1H), 1.97-1.59 (m, 6H), 1.57-1.14 (m, 16H), 1.10-0.93 (m, 4H), 0.84 (s,3H).

Synthesis of 494.4

To a suspension of [triphenyl(propyl)-k-phosphanyl]-k-bromide (16.5 g,42.9 mmol) in THF (70 mL) was added t-BuOK (4.80 g, 42.9 mmol) at 25° C.under N₂. After stirring at 60° C. for 1 h, 494.3 (5 g, 14.3 mmol) inTHF (20 mL) was added. After stirring at 60° C. for 16 h, the resultingsolution was quenched with NH₄Cl (100 mL) and extracted by EtOAc (3×40mL). The combined organic phase was washed with brine (2×100 mL), driedover anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (5% of EtOAc in PE) to give 494.4 (2.3 g) as asolid.

¹H NMR (400 MHz, CDCl₃) δ 5.00 (br t, J=7.4 Hz, 1H), 3.53 (q, J=6.9 Hz,2H), 3.48-3.35 (m, 2H), 2.67 (s, 1H), 2.36 (br dd, J=8.8, 16.6 Hz, 1H),2.24-2.01 (m, 4H), 1.98-1.60 (m, 5H), 1.53-1.27 (m, 9H), 1.25-1.10 (m,7H), 0.96-0.89 (m, 7H), 0.84 (s, 3H).

Synthesis of 494.5

To a solution of 494.4 (2.2 g, 5.87 mmol) in THF (30 mL) was addedBH₃/Me₂S (2.92 mL, 10 M, 29.3 mmol) dropwise at 25° C. under N₂. Afterstirring at 25° C. for 2 h, the mixture was cooled, quenched by EtOH(3.41 mL, 58.7 mmol, 0.79 g/mL) at 0° C. and dropwise with NaOH (11.7mL, 5M, 58.7 mmol). After addition, H₂O₂ (11.7 mL, 10 M, 117 mmol) wasadded slowly until the inner temperature no longer rises and the innertemperature was maintained below 0° C. The mixture was poured intoaqueous Na₂S₂O₃ (30 mL, sat.), stirred for 30 min and extracted withEtOAc (2×30 mL). The combined organic solution was washed with water(2×30 mL), dried over anhydrous Na₂SO₄, filtered and concentrated togive 494.5 (2.2 g) as oil.

Synthesis of 494.6

To a solution of 494.5 (2.20 g, 5.60 mmol) in DCM (50 mL) was added DMP(4.74 g, 11.2 mmol) at 25° C. After stirring at 25° C. for 1 h, themixture was quenched with NaHCO₃ (50 mL) and Na₂S₂O₃ (50 mL, sat.) andextracted with DCM (2×50 mL). The combined organic phase was washed withNaHCO₃ (100 mL), Na₂S₂O₃ (100 mL, sat.) and brine (100 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜ 15% of EtOAc in PE) to give 494.6 (1.30 g, 60%) as anoil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.53 (q, J=6.5 Hz, 2H), 3.47-3.36 (m, 2H),2.72 (s, 1H), 2.55-2.49 (m, 1H), 2.37 (q, J=7.1 Hz, 2H), 2.24-2.09 (m,1H), 2.02-1.77 (m, 4H), 1.75-1.58 (m, 4H), 1.49-1.16 (m, 15H), 1.07-0.96(m, 4H), 0.93 (s, 3H), 0.57 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₂₅H₄₁O₂[M−H₂O+H]⁺373.3 found 373.3.

Synthesis of 494.7

To a solution of 494.6 (800 mg, 2.04 mmol) in toluene (15 mL) were addedTsOH (17.5 mg, 0.102 mmol), 1-phenylmethanamine (1.09 g, 10.2 mmol), andTi(OCH₂CH₃)₄ (2.32 g, 10.2 mmol). After stirring at 110° C. for 16 h,MeOH (10 mL) and borane sodium hydride (627 mg, 16.6 mmol) were added inone portion at 25° C. After stirring at 25° C. for 16 h, the resultingsolution was poured into water (50 mL) and filtered. The aqueous phasewas extracted with EtOAc (3×40 mL). The combined organic phase waswashed with brine (2×40 mL), dried over anhydrous Na₂SO₄, filtered andconcentrated. The residue was purified by flash column (0˜ 15% of EtOAcin PE) to give 494.7 (800 mg) as an oil.

Synthesis of 494.8

To a solution of 494.7 (800 mg, 1.66 mmol) in EtOH (50 mL) was addedPd/C (dry, 400 mg) under N₂. The suspension was degassed under vacuumand purged with H₂ for three times. After stirring under H₂ (50 psi) at25° C. for 16 h, the resulting suspension was filtered through a pad ofCelite and washed with THF (3×10 mL). The filtrate was concentrated togive 494.8 (480 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.53 (q, J=6.9 Hz, 2H), 3.47-3.35 (m, 2H),2.62 (br s, 1H), 2.12-1.64 (m, 8H), 1.64-1.31 (m, 13H), 1.30-1.16 (m,9H), 1.15-0.84 (m, 7H), 0.72 (s, 3H).

Synthesis of 494 & 495

To a solution of 5-bromopyridine-2-carbonitrile (65.1 mg, 0.356 mmol) intoluene (2 mL) were added 494.8 (200 mg, 0.510 mmol), Cs₂CO₃ (498 mg,1.52 mmol) and BINAP (62.7 mg, 0.101 mmol) under N₂. After stirring at25° C. for 20 mins, Pd(OAc)₂ (22.6 mg, 0.101 mmol) was added. Afterstirring at 110° C. for 16 h, the reaction was filtered andconcentrated. The residue was purified by flash column (0˜ 30% of EtOAcin PE) to give 494 (30 mg) and 495 (50 mg, 19.9%) both as solids. 494(30 mg, 0.0607 mmol) was further purified by SFC (Column DAICELCHIRALCEL OJ-H (250 mm*30 mm, 5 um); Condition 0.1% NH₃H₂O ETOH Begin B25%; End B 25% Gradient Time(min); 100% B Hold Time(min)FlowRate(ml/min) δ0) to give 494 (5.4 mg, 2%) as a solid.

494: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.95 (d, J=2.8 Hz, 1H), 7.47 (d,J=8.5 Hz, 1H), 6.76 (dd, J=2.8, 8.8 Hz, 1H), 3.99 (br d, J=8.5 Hz, 1H),3.52 (q, J=7.0 Hz, 2H), 3.46-3.34 (m, 2H), 3.30 (br d, J=8.3 Hz, 1H),2.76 (s, 1H), 1.98-1.60 (m, 7H), 1.52-1.30 (m, 10H), 1.29-1.03 (m, 10H),1.02-0.93 (m, 1H), 0.91 (s, 3H), 0.86 (t, J=7.4 Hz, 3H), 0.82 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₁H₄₆N₃O [M−H₂O+H]⁺476.3 found476.3.

495: ¹H NMR (400 MHz, CDCl3) δ_(H) 7.95 (d, J=2.8 Hz, 1H), 7.42 (d,J=8.8 Hz, 1H), 6.76 (dd, J=2.9, 8.7 Hz, 1H), 3.98 (br d, J=9.3 Hz, 1H),3.53 (q, J=7.0 Hz, 2H), 3.47-3.30 (m, 3H), 2.73 (s, 1H), 1.98-1.56 (m,9H), 1.50-1.05 (m, 19H), 1.01-0.79 (m, 6H), 0.58 (s, 3H). LC-ELSD/MSpurity 99%, MS ESI calcd. for C₃₁H₄₆N₃O [M−H₂O+H]⁺476.3 found 476.3.

Example 496: Synthesis of3-(((S)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)amino)benzonitrile(496)

To a solution of 213 (370 mg, 0.815 mmol) in EtOH (20 mL) was added Pd—C(dry, 200 mg) and one drop NH₃H₂O. After stirring under H₂ (50 psi) at50° C. for 48 h, the resulting suspension was filtered through a pad ofCelite and washed with EtOH (3×20 mL). The filtrate was concentrated togive 496.1 (300 mg) as an oil.

¹H NMR (400 MHz, CDCl₃) δ_(H)3.42-3.36 (m, 5H), 2.35 (s, 1H), 2.10-1.51(m, 12H), 1.50-1.20 (m, 11H), 1.20-0.93 (m, 8H), 0.87-0.64 (m, 4H).

Synthesis of 496

To a solution of 496.1 (200 mg, 0.55 mmol), 3-bromobenzonitrile (198 mg,1.09 mmol), BINAP (34.2 mg, 0.055 mmol) and Cs₂CO₃ (355 mg, 1.09 mmol)in toluene (5 mL) was added Pd(OAc)₂ (12.3 mg, 0.055 mmol) under N₂.After stirring at 120° C. for 18 h, water (10 mL) was added to theresulting solution and extracted with EtOAc (3×20 mL). The combinedorganic phase was washed with water (3×20 mL), brine (50 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified bycolumn (10% 30% of EtOAc in PE) to give 496 (73 mg) as an oil, which wasfurther purified by SFC (Column: DAICEL CHIRALCEL OD-H (250 mm*30 mm, 5um), Condition: 0.1% NH₃H₂O ETOH, Begin B: 30%, End B: 30%) to give 496(33.8 mg) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H)7.26 (t, J=8 Hz, 1H), 6.88-6.86 (m, 1H),6.70-6.67 (m, 2H), 3.59-3.57 (m, 1H), 3.41-3.34 (m, 5H), 3.28-3.22 (m,1H), 2.58 (s, 1H), 1.93-1.50 (m, 12H), 1.50-1.10 (m, 12H), 1.10-0.96 (m,2H), 0.86-0.82 (m, 6H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₃₀H₄₅N₂O₂ [M+H]⁺ 465 found 465.

Example 497: Synthesis of3-(((R)-1-((3R,5R,8R,9R,10S,13S,14S,17S)-3-hydroxy-3-(methoxymethyl)-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)propyl)amino)benzonitrile(497)

To a solution of 214 (625 mg, 1.37 mmol) in EtOH (20 mL) was added Pd/C(dry, 200 mg) and one drop NH₃H₂O. After stirring under H₂ (50 psi) at50° C. for 48 h, the resulting suspension was filtered through a pad ofCelite and washed with EtOH (3×10 mL). The filtrate was concentrated togive 497.1 (520 mg) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.42-3.34 (m, 5H), 3.00-2.70 (m, 2H), 2.34(s, 1H), 1.98-1.51 (m, 10H), 1.50-1.17 (m, 11H), 1.16-0.88 (m, 8H),0.77-0.54 (m, 4H).

Synthesis of 497

To a solution of 497.1 (200 mg, 0.55 mmol), 3-bromobenzonitrile (198 mg,1.09 mmol), BINAP (34.2 mg, 0.055 mmol) and Cs₂CO₃ (355 mg, 1.09 mmol)in toluene (5 mL) was added Pd(OAc)₂ (12.3 mg, 0.055 mmol) at 25° C.under N₂. After stirring at 120° C. for 18 h, the resulting solution wastreated with water (10 mL) and extracted with EtOAc (3×10 mL). Thecombined organic phase was washed with water (3×20 mL), brine (50 mL),dried over anhydrous Na₂SO₄, filtered and concentrated. The residue waspurified by flash column (10%˜ 30% of EtOAc in PE) to 497 (100 mg) as anoil, which was further purified by SFC (Column: DAICEL CHIRALCEL OD-H(250 mm*30 mm, Sum), Condition: 0.1% NH₃H₂O ETOH, Begin B: 35%, End B:35%) and then HPLC separation (column: Xtimate C18 150*40 mm*10 um,condition: water (0.225% FA)-ACN, Begin B: 93, End B: 97) to give 497(26.2 mg, 48%) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.16 (t, J=8 Hz, 1H), 6.85-6.83 (m, 1H),6.72-6.67 (m, 2H), 3.62-3.59 (m, 1H), 3.41-3.31 (m, 6H), 2.61 (s, 1H),1.89-1.51 (m, 10H), 1.50-1.25 (m, 9H), 1.21-0.81 (m, 10H), 0.60 (s, 3H).LC-ELSD/MS purity 99%, MS ESI calcd. for C₃₀H₄₅N₂O₂ [M+H]⁺ 465 found465.

Examples 498-501: Synthesis of1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)butyl)-1H-pyrazole-3-carbonitrile(498) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)butyl)-1H-pyrazole-3-carbonitrile(499) &1-((R)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)butyl)-1H-pyrazole-5-carbonitrile(500) &1-((S)-2-((3R,5R,8R,9R,10S,13S,14S,17R)-3-(ethoxymethyl)-3-hydroxy-13-methylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)butyl)-1H-pyrazole-5-carbonitrile(501)

Synthesis of 498.1

To a mixture of MePPh₃Br (20 g, 56.2 mmol) in THF (80 mL) was addedt-BuOK (6.30 g, 56.2 mmol) at 25° C. under N₂. After stirring at 50° C.for 30 min, 493.4 (8.5 g, 22.5 mmol) in THF (15 mL) was added inportions at 50° C. After stirring at 50° C. for 3 h, the reactionmixture was quenched with 10% NH₄Cl aqueous (200 mL) at 15° C. andextracted with EtOAc (2×200 mL). The combined organic phase wasconcentrated under vacuum. The residue was purified by flash column(0˜10% of EtOAc in PE) twice to give 498.1 (8 g, 94.1%, with 6.6% ofepimer at C3 by H NMR) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 4.87-4.82 (m, 1H), 4.75 (s, 1H), 4.11 (d,J=7.3 Hz, 1H), 3.55-3.37 (m, 4H), 2.11-1.97 (m, 4H), 1.84-1.55 (m, 8H),1.49-1.32 (m, 6H), 1.27-1.09 (m, 9H), 1.07-0.97 (m, 5H), 0.55 (s, 3H).

Synthesis of 498.2

To a solution of 498.1 (8 g, 21.3 mmol) in THF (80 mL) was added BH₃Me₂S(6.39 mL, 10 M, 63.9 mmol) at 0° C. After stirring at 20° C. for 3 h,the reaction was sequentially treated with EtOH (15 mL) at 25° C., NaOH(42.6 mL, 5 M, 213 mmol) at 0° C. and H₂O₂ (27.6 mL, 276 mmol, 10 M)slowly. After stirring at 70° C. for 1 h, the reaction mixture wasextracted with EtOAc (3×80 mL). The combined organic layer was washedwith Na₂S₂O₃ (100 mL, sat.), brine (80 mL), dried over anhydrous Na₂SO₄,filtered and concentrated. The residue was purified by flash column(0˜20% of EtOAc in PE) to give 498.2 (7.5 g) as a solid.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.73-3.51 (m, 2H), 3.63-3.38 (m, 5H), 2.04(s, 2H), 1.95-1.70 (m, 4H), 1.69-1.48 (m, 6H), 1.45-1.22 (m, 9H),1.26-1.21 (m, 1H), 1.20 (t, J=7.0 Hz, 4H), 1.14-0.98 (m, 5H), 0.94-0.81(m, 3H), 0.70-0.65 (m, 3H).

Synthesis of 498.3

To a solution of 498.2 (2 g, 5.09 mmol) in DCM (20 mL) were added PPh₃(1.71 g, 6.51 mmol) and NBS (1.16 g, 6.51 mmol) at 0° C. After stirringat 25° C. for 2 h, the reaction was added to water (20 mL) and extractedwith DCM (2×20 mL). The combined organic phase was washed with brine (20mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residuewas purified by flash column (0˜10% of EtOAc in PE) to give 498.3 (1.5g, 64.9%, with 7% of epimer at C3 by H NMR) as oil.

¹H NMR (400 MHz, CDCl₃) δ_(H) 3.53 (d, J=7.0 Hz, 3H), 3.42 (d, J=12.0Hz, 2H), 1.84-1.70 (m, 5H), 1.67-1.46 (m, 9H), 1.45-1.31 (m, 7H),1.30-1.08 (m, 7H), 0.98-0.78 (m, 7H), 0.70-0.65 (m, 3H).

Synthesis of 498.4 & 498.5

To a solution of 498.3 (1.3 g, 2.85 mmol) and Cs₂CO₃ (1.85 g, 5.70 mmol)in DMF (8 mL) was added 1H-pyrazole-3-carbonitrile (530 mg, 5.70 mmol)at 25° C. under N₂. After stirring at 80° C. for 16 h, the reaction waspoured into water (10 mL) and extracted with EtOAc (3×10 mL). Thecombined organic phase was washed with brine (2×10 mL), dried overanhydrous Na₂SO₄, filtered and concentrated. The residue was purified byflash column (0˜20% of EtOAc in PE) to give 498.4 (1 g, with 8% ofepimer at C3 by H NMR) and 498.5 (500 mg) both as a solid.

498.4: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.56 (s, 1H), 6.79-6.75 (m, 1H),3.56-3.38 (m, 4H), 2.69 (s, 1H), 1.94-1.68 (m, 9H), 1.67-1.55 (m, 2H),1.50-1.35 (m, 6H), 1.31-1.18 (m, 7H), 1.16-0.95 (m, 8H), 0.91-0.81 (m,1H). LC-ELSD/MS purity 66.5%; MS ESI calcd. for C₂₉H₄₄N₃O [M+H—H₂O]⁺450,found 450.

498.5: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.69 (d, J=2.5 Hz, 2H), 7.43-7.40(m, 1H), 6.75 (d, J=2.5 Hz, 2H), 6.66-6.63 (m, 1H), 4.38 (dd, J=4.8,13.6 Hz, 1H), 4.20-4.08 (m, 1H), 4.11 (d, J=7.0 Hz, 2H), 3.96 (dd,J=10.4, 13.4 Hz, 1H), 3.57-3.40 (m, 4H), 1.91-1.70 (m, 5H), 1.67-1.54(m, 3H), 1.48-1.32 (m, 8H), 1.15-1.00 (m, 7H), 0.85-0.73 (m, 6H), 0.70(s, 1H). LC-ELSD/MS purity 33.5%; MS ESI calcd. for C₂₉H₄₄N₃O[M+H—H₂O]⁺450, found 450.

Separation of 498 & 499 & 500 & 501

498.4 (1 g, 2.13 mmol) was separated by SFC (Column: DAICEL CHIRALPAK AD(250 mm*30 mm, 10 um), Condition: 0.1% NH₃H₂O ETOH, Begin B: 40%, End B:40%, FlowRate (ml/min):70) to afford 498 (Rt=3.387 min, 580 mg, 38.9%) &499 (Rt=2.535 min, 340 mg, 22.8%).

498.5 (500 mg, 1.07 mmol) was separated by SFC (Column: DAICEL CHIRALPAKAD (250 mm*30 mm, 10 um), Condition: 0.1% NH₃H₂O ETOH, Begin B: 40%, EndB: 40%, FlowRate (ml/min):70) to afford 500 (Rt=1.511 min, 150 mg, 10%)and 501 (Rt=2.635 min, 100 mg, 6.71%).

500 (150 mg, 0.320 mmol) was further purified by SFC (Column: DAICELCHIRALPAK AS-H (250 mm*30 mm, Sum), Condition: 0.1% NH₃H₂O ETOH, BeginB: 20%, End B: 20%, FlowRate (ml/min):60) to afford 500 (97 mg, 65.1%)as a solid.

501 (100 mg, 0.213 mmol) was further purified by SFC (Column: DAICELCHIRALPAK AD-H (250 mm*30 mm, Sum), Condition: 0.1% NH₃H₂O ETOH, BeginB: 40%, End B: 40%, FlowRate (ml/min):50) to afford 501 (38 mg, 38.0%)as a solid.

498: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.42-7.38 (m, 1H), 7.26 (s, 1H),6.66-6.63 (m, 1H), 4.38 (dd, J=4.8, 13.6 Hz, 1H), 3.95 (dd, J=10.7, 13.4Hz, 1H), 3.56-3.38 (m, 4H), 2.71 (s, 1H), 2.13-2.04 (m, 1H), 1.87-1.70(m, 5H), 1.68-1.55 (m, 2H), 1.49 (br s, 1H), 1.45-1.18 (m, 13H),1.16-1.00 (m, 7H), 0.83-0.73 (m, 6H). LC-ELSD/MS purity 99%; MS ESIcalcd. for C₂₇H₃₈N₃ [M+H—H₂O-EtOH]⁺404, found 404; C₂₉H₄₄N₃O[M+H—H₂O]⁺450, found 450; C₂₉H₄₅N₃O₂Na [M+Na]⁺490, found 490. analyticSFC 100% de.

499: ¹H NMR (400 MHz, CDCl₃) δ_(H)7.40 (s, 1H), 7.26 (s, 1H), 6.65 (s,1H), 4.22 (dd, J=4.1, 13.7 Hz, 1H), 3.98 (dd, J=9.8, 13.6 Hz, 1H),3.56-3.38 (m, 3H), 3.46-3.36 (m, 1H), 2.69 (s, 1H), 2.01 (br s, 1H),1.93-1.86 (m, 2H), 1.86-1.71 (m, 3H), 1.67-1.55 (m, 3H), 1.50-1.32 (m,9H), 1.27-1.16 (m, 5H), 1.15-0.99 (m, 6H), 0.84 (t, J=7.4 Hz, 3H), 0.71(s, 3H). LC-ELSD/MS purity 99%; MS ESI calcd. for C₂₉H₄₄N₃O[M+H—H₂O]⁺450, found 450; C₂₉H₄₅N₃O₂Na [M+Na]⁺490, found 490. analyticSFC 98% de.

500: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.56 (s, 1H), 6.78 (s, 1H), 4.46 (dd,J=5.2, 13.7 Hz, 1H), 4.15 (dd, J=10.9, 13.6 Hz, 1H), 3.53 (d, J=7.0 Hz,2H), 3.42 (d, J=9.1 Hz, 2H), 2.70 (s, 1H), 2.18 (br s, 1H), 1.84 (br s,3H), 1.75 (s, 2H), 1.68-1.59 (m, 5H), 1.48-1.30 (m, 7H), 1.21 (t, J=7.0Hz, 5H), 1.16-0.96 (m, 7H), 0.86-0.78 (m, 6H). LC-ELSD/MS purity 99%; MSESI calcd. for C₂₉H₁₄₄N₃O [M+H−H₂O]⁺450, found 450; C₂₉H₄₅N₃O₂Na[M+Na]⁺490, found 490. analytic SFC 100% de.

501: ¹H NMR (400 MHz, CDCl₃) δ_(H) 7.56 (s, 1H), 6.77 (s, 1H), 4.26 (d,J=4.1 Hz, 1H), 4.18 (d, J=10.6 Hz, 1H), 3.56-3.38 (m, 4H), 2.69 (s, 1H),1.95-1.71 (m, 5H), 1.70-1.59 (m, 7H), 1.59-1.34 (m, 8H), 1.31-1.16 (m,3H), 1.15-0.99 (m, 7H), 0.85 (t, J=7.4 Hz, 3H), 0.73 (s, 3H). LC-ELSD/MSpurity 99%; MS ESI calcd. for C₂₉H₄₄N₃O [M+H−H₂O]⁺450, found 450;C₂₉H₄₅N₃O₂Na [M+Na]⁺490, found 490. analytic SFC 100% de.

The following examples were synthesized similar to Example 33 with thelisted aryl halide and appropriate SM. In the case of diasteriomericproducts, typically the diastereomeric isomers were separated by SFC(e.g. Column: DAICEL CHIRALPAK AS-H (250 mm*30 mm, 5 um), Condition:0.1% NH₃H₂O EtOH, Begin B: 30%, End B 30%) or prep-HPLC (column:DuraShell 150*25 mm*5 um; Condition: water (10 mM NH₄HCO₃)-ACN; 75%-95%in 7 min. FlowRate: 25 mL/min) yielding both diastereomers afterseparation. The diastereomers were assigned based on 1H NMR of C21-Me.

Example SM Aryl halide STRUCTURE Analytical 502 77.2 1-(6-fluoropyridin-3- yl)-1H-pyrazole- 3-carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.31 (d, J = 2.8 Hz, 1H), 7.78 (d, J = 2.5Hz, 1H), 7.70 (dd, J = 2.8, 9.0 Hz, 1H), 6.83 (d, J = 2.5 Hz, 1H), 6.39(d, J = 9.0 Hz, 1H), 4.51 (br d, J = 9.3 Hz, 1H), 3.81 (br s, 1H), 3.53(q, J = 7.0 Hz, 2H), 3.46-3.35 (m, 2H), 2.74 (s, 1H), 2.01- 1.77 (m,4H), 1.65 (br s, 3H), 1.54-1.27 (m, 9H), 1.25-1.05 (m, 12H), 1.02- 0.93(m, 1H), 0.90 (s, 3H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESIcalcd. for C₃₃H₄₈N₅O₂ [M + H]⁺ 546.4 found 546.4. 503 492.4 5-bromopyridine- 2-carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.96 (d, J = 2.4 Hz, 1H), 7.42 (d, J = 8.4Hz, 1H), 6.77 (dd, J = 2.8, 8.7 Hz, 1H), 3.99 (br d, J = 9.2 Hz, 1H),3.52 (q, J = 6.8 Hz, 2H), 3.41 (q, J = 9.2 Hz, 3H), 1.88-1.72 (m, 6H),1.69-1.60 (m, 3H), 1.50-1.29 (m, 10H), 1.27- 1.10 (m, 8H), 1.09-0.91 (m,3H), 0.86 (t, J = 7.2 Hz, 2H), 0.82-0.79 (m, 1H), 0.59 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd for C₃₀H₄₄N₃O[M − H₂O + H]⁺462, found462; C₃₀H₄₆N₃O₂ [M + H]⁺ 480, found 480; C₃₀H₄₅N₃O₂Na[M + Na]⁺502, found502. 504 77.2 1-(6- bromopyridin-3- yl)-1H-pyrazole- 4-carbonitrile

¹H NMR (400 MHz, CDCl₃) δ_(H) 8.29-8.28 (d, J = 4 Hz, 1H), 8.10 (s, 1H),7.95 (s, 1H), 7.67-7.64 (m, 1H), 3.81 (s, 1H), 3.55- 3.37 (m, 2H), 2.73(s, 1H), 1.96-1.55 (m, 11H), 1.49- 1.30 (m, 9H), 1.25-1.06 (m, 13H). 0.9(s, 3H), 0.63 (s, 3H). LC-ELSD/MS purity 99%, MS ESI calcd. forC₃₃H₄₈N₅O₂ [M + H]⁺ 546, found 546. 505 489.4 bromobenzene

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.19 (t, J = 4 Hz, 2H), 6.73 (t, J = 8 Hz,1H), 6.60-5.58 (d, J = 8 Hz, 2H), 3.86-3.77 (m, 1H), 3.52 (d, J = 12 Hz,1H), 1.99-1.94 (m, 1H), 1.84-1.56 (m, 9H), 1.48-1.25 (m, 13H), 1.19-0.92(m, 5H), 0.62 (s, 3H). ¹⁹F NMR (400 MHz, CDCl₃) δ_(F) −72.953. LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₃₉F₃NO [M + H]⁺ 450, found450. 506

¹H NMR (400 MHz, CDCl₃) δ_(H) 7.19 (t, J = 8 Hz, 2H), 6.75 (t, J Hz,1H), 6.64 (d, J Hz, 2H), 3.99- 3.90 (m, 1H), 1.87-1.55 11H), 1.48-1.26(m, 12H), 1.22-0.96 (m, 6H), 0.58 (s, 3H). ¹⁹F NMR (400 MHz, CDCl₃)δ_(F) −74.740. LC-ELSD/MS purity 99%, MS ESI calcd. for C₂₇H₃₈F₃NO [M +H]⁺ 450, found 450.

Example 507: Steroid Inhibition of TBPS Binding

[³⁵S]-t-Butylbicyclophosphorothionate (TBPS) binding assays using ratbrain cortical membranes in the presence of 5 mM GABA has been described(Gee et al, J. Pharmacol. Exp. Ther. 1987, 241, 346-353; Hawkinson etal, Mol. Pharmacol. 1994, 46, 977-985; Lewin, A. H et al., Mol.Pharmacol. 1989, 35, 189-194).

Briefly, cortices are rapidly removed following decapitation of carbondioxide-anesthetized Sprague-Dawley rats (200-250 g). The cortices arehomogenized in 10 volumes of ice-cold 0.32 M sucrose using aglass/teflon homogenizer and centrifuged at 1500×g for 10 min at 4° C.The resultant supernatants are centrifuged at 10,000×g for 20 min at 4°C. to obtain the P2 pellets. The P2 pellets are resuspended in 200 mMNaCl/50 mM Na—K phosphate pH 7.4 buffer and centrifuged at 10,000×g for10 min at 4° C. This washing procedure is repeated twice and the pelletsare resuspended in 10 volumes of buffer. Aliquots (100 mL) of themembrane suspensions are incubated with 3 nM [³⁵S]-TBPS and 5 mLaliquots of test drug dissolved in dimethyl sulfoxide (DMSO) (final0.5%) in the presence of 5 mM GABA. The incubation is brought to a finalvolume of 1.0 mL with buffer. Nonspecific binding is determined in thepresence of 2 mM unlabeled TBPS and ranged from 15 to 25%. Following a90 min incubation at room temp, the assays are terminated by filtrationthrough glass fiber filters (Schleicher and Schuell No. 32) using a cellharvester (Brandel) and rinsed three times with ice-cold buffer. Filterbound radioactivity is measured by liquid scintillation spectrometry.Non-linear curve fitting of the overall data for each drug averaged foreach concentration is done using Prism (GraphPad). The data are fit to apartial instead of a full inhibition model if the sum of squares issignificantly lower by F-test. Similarly, the data are fit to a twocomponent instead of a one component inhibition model if the sum ofsquares is significantly lower by F-test. The concentration of testcompound producing 50% inhibition (IC₅₀) of specific binding and themaximal extent of inhibition (I_(max)) are determined for the individualexperiments with the same model used for the overall data and then themeans±SEM.s of the individual experiments are calculated. Picrotoxinserves as the positive control for these studies as it has beendemonstrated to robustly inhibit TBPS binding.

Various compounds are or can be screened to determine their potential asmodulators of [³⁵S]-TBPS binding in vitro. These assays are or can beperformed in accordance with the above

In Table 2 below, A indicates a TBPS IC₅₀ (μM)<0.1 μM, B indicates aTBPS IC₅₀ (μM) of 0.1 μM to <1 μM, C indicates a TBPS IC₅₀ (μM) of 1 μMto <10 μM, D indicates a TBPS IC₅₀ (μM) of ≥10 μM.

TABLE 2 Example IC₅₀ 1 C 2 B 3 A 4 B 5 A 6 C 7 C 8 A 9 B 10 B 11 B 12 A13 A 14 B 15 A 16 A 17 C 18 A 19 C 20 B 21 C 22 A 23 C 24 A 25 A 26 C 29B 30 C 31 B 32 C 33 A 34 A 35 A 36 A 37 A 38 B 39 C 40 A 41 B 42 B 43 B44 A 45 A 46 A 47 A 48 A 49 A 50 A 51 A 52 A 53 B 54 B 55 A 56 B 57 B 58C 59 B 60 B 61 A 62 A 63 A 64 A 65 A 66 A 67 A 68 A 69 A 70 A 71 B 72 A73 A 74 A 75 A 76 B 77 A 78 A 87 A 88 A 90 B 91 B 92 A 100 B 101 C 102 C103 A 103 A 104 A 105 C 106 B 107 C 108 B 109 A 110 C 111 A 112 B 113 B114 C 115 C 116 A 117 C 118 B 119 C 120 A 121 C 122 B 123 D 124 B 125 B126 C 127 B 128 B 129 B 130 A 131 A 132 A 133 A 134 A 135 C 150 A 151 C152 C 153 C 154 C 155 C 156 A 157 C 158 B 159 D 160 C 161 C 162 B 163 C164 C 201 B 202 A 203 A 204 B 205 C 206 B 207 C 208 B 209 B 210 C 211 B212 C 213 C 214 C 250 A 251 B 252 A 253 C 254 A 255 C 256 A 257 B 258 B259 A 260 A 261 B 262 A 263 A 264 B 265 B 266 B 267 A 268 A 269 A 270 A271 A 272 A 273 A 274 B 275 A 276 A 278 A 279 A 280 A 282 B 283 B 285 A286 A 287 A 288 A 289 A 290 A 293 A 294 A 295 A 296 A 297 A 298 A 299 A300 A 301 A 302 A 303 A 304 A 305 A 306 A 307 A 308 A 309 A 310 B 311 A312 A 313 A 314 B 315 A 316 A 317 A 318 A 319 A 320 A 321 A 322 A 323 A324 A 325 A 326 A 327 B 328 B 329 D 330 D 331 D 332 B 333 B 334 D 335 B336 D 337 B 338 B 339 A 340 A 341 A 342 B 343 B 344 D 345 C 346 C 347 C348 C 349 D 350 D 351 C 352 C 353 C 354 C 355 A 356 A 357 B 358 B 359 D360 C 362 C 363 C 364 B 365 A 400 A 401 A 402 A 403 B 404 A 405 A 406 A407 A 410 B 411 A 412 B 413 A 414 A 415 A 416 B 417 A 418 C 420 A 421 B422 A 423 B 424 A 425 C 426 B 427 A 428 A 429 A 430 B 431 B 432 A 433 A434 B 435 A 436 B 437 A 438 B 439 A 440 A 441 A 442 B 443 A 444 B 445 A446 A 449 A 450 B 451 B 453 A 454 B 455 A 456 B 487 B 488 C 489 C 490 B491 B 492 C 493 C 494 B 495 B 496 B 497 B 498 B 499 A 500 C 501 B 502 B503 B 504 B 505 B

Example 508: α1β2γ2 and α4β3δ SyncroPatch Assay

The SyncroPatch 384PE (Nanion, Germany) automated patch clamp platformwas used to obtain recordings from human embryonic kidney cells stablyexpressing human GABA_(A) receptors composed of the α1β2γ2 and α4β3δsubunits. Cells were bathed in an extracellular solution containing: 140mM NaCl, 4 mM KCl, 2 mM CaCl₂), 1 mM MgCl₂, 10 mM HEPES, 5 mM glucoseand 0.1% DMSO. Solution was maintained at pH 7.4 with NaOH and 300mOsm/L. Chips with multi-hole (4×) medium resistance (2-3M) are used forthe recombinant cell line experiments. Once harvested, cells are storedin extracellular solution set to 10° C. in the cell hotel with a minimumshaking speed of 200 rpm before addition to the chip at the beginning ofeach experiment. Whole cell patch clamp recordings were made using anintracellular solution containing: 90 mM KCl, 50 mM KF, 1.5 mM MgCl₂,11.1 mM EGTA and 10 mM HEPES, 2 mM NaATP, maintained at pH 7.2 with KOH.Currents are leak corrected (using P/2 leak correction protocol) andsampled at 5 kHz. Cells were clamped at −120 mV. After establishing astable baseline, a submaximal concentration of GABA (3-5 μM for cellsexpressing α1β2γ2 and 10 μM for cells expressing α1β2γ2) was applied ata rate of 10 μl/second, immediately followed by application ofGABA+compound (10 μl/second) at a single concentration of compound percell. Compounds were tested at six concentrations (0.029, 0.12, 0.47,1.88, 7.5, and 30 μM) in n=8-12 cells per concentration. Compound effectwas determined by dividing peak current amplitude after GABA+compound bypeak current amplitude after GABA alone. EC₅₀ and E_(max) values weredetermined from average values for each concentration.

In Table 3 below, for α1β2γ2 EC₅₀ and α4β3δ EC₅₀, A indicates an EC₅₀(M)<0.2 μM, B indicates an EC₅₀ (M) of 0.2 μM to <1 μM, C indicates anEC₅₀ (μM) of ≥1 M.

For α1β2γ2 E_(max), A indicates an E_(max) response <8, B indicates anE_(max) response 8 to <10, C indicates an E_(max) response ≥10. Forα4β3δ E_(max), A indicates an E_(max) response <30, B indicates anE_(max) response 30 to <45, C indicates an E_(max) response ≥45.

TABLE 3 α1β2γ2 α1β2γ2 α4β3δ α4β3δ Example EC₅₀ E_(MAX) EC₅₀ E_(MAX) 41 CA C B 53 C A C A 61 A C A A 65 C A A B 72 B C B C 132 C C C B 263 B A BA 270 B C B C 272 A C A A 276 B B A A 278 A A A A 280 A C A B 286 A B BA 287 B B B A 293 C A B A 294 B C B B 299 C A B 303 A C A B 311 A C B B320 B B B C 323 A C B B 332 A A B A 339 C C A C 341 A A A A 357 B B B B358 C A C B 365 C B B B 401 C C C A 405 C C A B 406 A A A B 407 A C C C413 A C A B 420 A B A B 430 B B A A 436 B A C B 438 B A C B 439 A C A C443 A C A B 453 B A A A 499 A A A A

EQUIVALENTS AND SCOPE

In the claims articles such as “a,” “an,” and “the” may mean one or morethan one unless indicated to the contrary or otherwise evident from thecontext. Claims or descriptions that include “or” between one or moremembers of a group are considered satisfied if one, more than one, orall of the group members are present in, employed in, or otherwiserelevant to a given product or process unless indicated to the contraryor otherwise evident from the context. The invention includesembodiments in which exactly one member of the group is present in,employed in, or otherwise relevant to a given product or process. Theinvention includes embodiments in which more than one, or all of thegroup members are present in, employed in, or otherwise relevant to agiven product or process.

Furthermore, the invention encompasses all variations, combinations, andpermutations in which one or more limitations, elements, clauses, anddescriptive terms from one or more of the listed claims is introducedinto another claim. For example, any claim that is dependent on anotherclaim can be modified to include one or more limitations found in anyother claim that is dependent on the same base claim. Where elements arepresented as lists, e.g., in Markush group format, each subgroup of theelements is also disclosed, and any element(s) can be removed from thegroup. It should it be understood that, in general, where the invention,or aspects of the invention, is/are referred to as comprising particularelements and/or features, certain embodiments of the invention oraspects of the invention consist, or consist essentially of, suchelements and/or features. For purposes of simplicity, those embodimentshave not been specifically set forth in haec verba herein. It is alsonoted that the terms “comprising” and “containing” are intended to beopen and permits the inclusion of additional elements or steps. Whereranges are given, endpoints are included. Furthermore, unless otherwiseindicated or otherwise evident from the context and understanding of oneof ordinary skill in the art, values that are expressed as ranges canassume any specific value or sub-range within the stated ranges indifferent embodiments of the invention, to the tenth of the unit of thelower limit of the range, unless the context clearly dictates otherwise.

This application refers to various issued patents, published patentapplications, journal articles, and other publications, all of which areincorporated herein by reference. If there is a conflict between any ofthe incorporated references and the instant specification, thespecification shall control. In addition, any particular embodiment ofthe present invention that falls within the prior art may be explicitlyexcluded from any one or more of the claims. Because such embodimentsare deemed to be known to one of ordinary skill in the art, they may beexcluded even if the exclusion is not set forth explicitly herein. Anyparticular embodiment of the invention can be excluded from any claim,for any reason, whether or not related to the existence of prior art.

Those skilled in the art will recognize or be able to ascertain using nomore than routine experimentation many equivalents to the specificembodiments described herein. The scope of the present embodimentsdescribed herein is not intended to be limited to the above Description,but rather is as set forth in the appended claims. Those of ordinaryskill in the art will appreciate that various changes and modificationsto this description may be made without departing from the spirit orscope of the present invention, as defined in the following claims.

What is claimed:
 1. A compound of Formula I:

or a pharmaceutically acceptable salt thereof; wherein:

represents a single or double bond, provided if a double bond ispresent, then one of R^(6a) or R^(6b) is absent and R⁵ is absent; L isselected from the group consisting of:

 wherein A indicates the point of attachment at C17; X is selected fromthe group consisting of —C(O)N(R^(55a))(R^(55b)), —N(R^(55a))(R^(55b)),—N(R^(55b))C(O)(R^(55a)), and R^(55c); R^(Y) is each independentlyhydrogen, cyano, haloalkyl, or unsubstituted alkyl; R^(55c) iscarbon-bound substituted or unsubstituted heteroaryl or substituted orunsubstituted aryl; R^(55a) and R^(55b) is each independently hydrogen,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, substituted orunsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, —OR^(A1), —N(R^(A1))₂—SR^(A1), —C(═O)R^(A1), —C(═O)OR^(A1),—C(═O)SR^(A1), —C(═O)N(R^(A1))₂, —OC(═O)R^(A1), —OC(═O)OR^(A1),—OC(═O)N(R^(A1))₂, —OC(═O)SR^(A1), —OS(═O)₂R^(A1), —OS(═O)₂OR^(A1),—OS(═O)₂N(R^(A1))₂, —N(R^(A1))C(═O)R^(A1), —N(R^(A1))C(═NR^(A1))R^(A1),—N(R^(A1))C(═O)OR^(A1), —N(R^(A1))C(═O)N(R^(A1))₂, —N(R^(A1))C(═NR^(A1))N(R^(A1))₂, —N(R^(A1))S(═O)₂R^(A1), —N(R^(A1))S(═O)₂OR^(A1),—N(R^(A1))S(═O)₂N(R^(A1))₂, —SC(═O)R^(A1), —SC(═O)OR^(A1),—SC(═O)SR^(A1), —SC(═O)N(R^(A1))₂, —S(═O)₂R^(A1), —S(═O)₂OR^(A1), or—S(═O)₂N(R^(A1))₂, wherein each instance of R^(A1) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl,substituted or unsubstituted heteroaryl, an oxygen protecting group whenattached to oxygen, a nitrogen protecting group when attached tonitrogen, a sulfur protecting group when attached to sulfur, or twoR^(A1) groups are taken with the intervening atoms to form a substitutedor unsubstituted heterocyclic ring; or R^(55a) and R^(55b) may jointogether with the intervening atoms to form a substituted orunsubstituted heterocyclyl or a substituted or unsubstituted heteroaryl;each of R^(1a), R^(1b), R^(2a), R^(2b), R^(4a), R^(4b), R^(7a), R^(7b),R^(11a), R^(11b), R^(12a), and R^(12b) is independently hydrogen,halogen, cyano, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, —OR^(A1), —N(R^(A1))₂, —SR^(A1), —C(═O)R^(A1),—C(═O)OR^(A1), —C(═O)SR^(A1), —C(═O)N(R^(A1))₂, —OC(═O)R^(A1),—OC(═O)OR^(A1), —OC(═O)N(R^(A1))₂, —OC(═O)SR^(A1), —OS(═O)₂R^(A1),—OS(═O)₂OR^(A1), —OS(═O)₂N(R^(A1))₂, —N(R^(A1))C(═O)R^(A1),—N(R^(A1))C(═NR^(A1))R^(A1), —N(R^(A1))C(═O)OR^(A1),—N(R^(A1))C(═O)N(R^(A1))₂, —N(R^(A1))C(═NR^(A1)) N(R^(A1))₂,—N(R^(A1))S(═O)₂R^(A1), —N(R^(A1))S(═O)₂OR^(A1),—N(R^(A1))S(═O)₂N(R^(A1))₂, —SC(═O)R^(A1), —SC(═O)OR^(A1),—SC(═O)SR^(A1), —SC(═O)N(R^(A1))₂, —S(═O)₂R^(A1), —S(═O)₂OR^(A1), or—S(═O)₂N(R^(A1))₂, wherein each instance of R^(A1) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl,substituted or unsubstituted heteroaryl, an oxygen protecting group whenattached to oxygen, a nitrogen protecting group when attached tonitrogen, or a sulfur protecting group when attached to sulfur; orR^(11a) and R^(11b) are joined to form an oxo (═O) group; or R^(12a) andR^(12b) are joined to form an oxo (═O) group; or R^(4a) and R^(4b) arejoined to form an oxo (═O) group; or R^(7a) and R^(7b) are joined toform an oxo (═O) group; or R^(2a) and R^(2b) are joined to form an oxo(═O) group; or R^(1a) and R^(1b) are joined to form an oxo (═O) group;R^(3a) is substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, substitutedor unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, or substituted or unsubstitutedheteroaryl; R⁵ is hydrogen or substituted or unsubstituted alkyl; eachof R^(6a) and R^(6b) is independently hydrogen, halogen, cyano, —NO₂,—OH, substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, or substituted or unsubstituted alkynyl; or R^(6a) and R^(6b)are joined to form an oxo (═O) group; R^(D) is independently hydrogen,halogen, —CN, —NO₂, oxo, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted carbocyclyl, substituted or unsubstitutedheterocyclyl, substituted or unsubstituted aryl, substituted orunsubstituted heteroaryl, —OR^(C3), —N(R^(C3))₂, —SR^(C3), —C(═O)R^(C3),—C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂, —OC(═O)R^(C3),—OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3), —OS(═O)₂R^(C3),—OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),—N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),—N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,—N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),—N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),—SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3), —S(═O)₂OR^(C3), or—S(═O)₂N(R^(C3))₂, wherein each instance of R^(C3) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl, an oxygen protecting group when attached tooxygen, a nitrogen protecting group when attached to nitrogen, or asulfur protecting group when attached to sulfur; R¹⁸ is substituted orunsubstituted alkyl; R¹⁹ is hydrogen, substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, or substituted orunsubstituted alkynyl; and q is an integer from 0 to 5; provided thatthe compound is not:

 or a pharmaceutically acceptable salt thereof.
 2. The compound of claim1, wherein the compound is a compound of Formula (I-I):

or a pharmaceutically acceptable salt thereof, wherein each of R^(15a),R^(15b), R^(16a), and R^(16b) is independently hydrogen, halogen, —CN,—NO₂, substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, substituted orunsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, —OR^(C3), —N(R^(C3))₂, —SR^(C3), —C(═O)R^(C3),—C(═O)OR^(C3), —C(═O)SR^(C3), —C(═O)N(R^(C3))₂, —OC(═O)R^(C3),—OC(═O)OR^(C3), —OC(═O)N(R^(C3))₂, —OC(═O)SR^(C3), —OS(═O)₂R^(C3),—OS(═O)₂OR^(C3), —OS(═O)₂N(R^(C3))₂, —N(R^(C3))C(═O)R^(C3),—N(R^(C3))C(═NR^(C3))R^(C3), —N(R^(C3))C(═O)OR^(C3),—N(R^(C3))C(═O)N(R^(C3))₂, —N(R^(C3))C(═NR^(C3)) N(R^(C3))₂,—N(R^(C3))S(═O)₂R^(C3), —N(R^(C3))S(═O)₂OR^(C3),—N(R^(C3))S(═O)₂N(R^(C3))₂, —SC(═O)R^(C3), —SC(═O)OR^(C3),—SC(═O)SR^(C3), —SC(═O)N(R^(C3))₂, —S(═O)₂R^(C3), —S(═O)₂OR^(C3), or—S(═O)₂N(R^(C3))₂, wherein each instance of R^(C3) is independentlyselected from hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted aryl, substituted or unsubstitutedheteroaryl, substituted or unsubstituted carbocyclyl, or substituted orunsubstituted heterocyclyl, an oxygen protecting group when attached tooxygen, a nitrogen protecting group when attached to nitrogen, a sulfurprotecting group when attached to sulfur; or R^(15a) and R^(15b) arejoined to form an oxo (═O) group; or R^(16a) and R^(16b) are joined toform an oxo (═O) group.
 3. The compound of claim 1, wherein the compoundis a compound of Formula (I-a):

or a pharmaceutically acceptable salt thereof, wherein the variables areas defined in claim
 1. 4. The compound of any one of claims 1-3, whereinthe compound is a compound of Formula (I-Ia):

or a pharmaceutically acceptable salt thereof, wherein the variables areas defined in claim 1 or
 2. 5. The compound of any one of claims 1-4,wherein L is


6. The compound of any one of claims 1-5, wherein L is


7. The compound of any one of claims 1-5, wherein L is


8. The compound of any one of claims 1-5, wherein L is


9. The compound of any one of claims 1-4, wherein L is


10. The compound of any one of claims 1-4, wherein L is


11. The compound of any one of claims 1-4, wherein L is


12. The compound of any one of claims 1-11, wherein X is—N(R^(55b))C(O)(R^(55a)).
 13. The compound of any one of claims 1-11,wherein X is —N(R^(55a))(R^(55b)).
 14. The compound of any one of claims1-11, wherein X is —C(O)N(R^(55a))(R^(55b)).
 15. The compound of any oneof claims 1-14, wherein R^(55a) is hydrogen or methyl and R^(55b) issubstituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, substituted orunsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl,substituted or unsubstituted aryl, or substituted or unsubstitutedheteroaryl.
 16. The compound of any one of claims 1-14, wherein R^(55a)and R^(55b) is each independently hydrogen, substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, substituted orunsubstituted alkynyl, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, substituted or unsubstitutedaryl, or substituted or unsubstituted heteroaryl.
 17. The compound ofany one of claims 1-14, wherein R^(55a) and R^(55b) is eachindependently hydrogen, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, substituted or unsubstitutedaryl, or substituted or unsubstituted heteroaryl.
 18. The compound ofany one of claims 1-14, wherein R^(55a) and R^(55b) is eachindependently substituted carbocyclyl, substituted heterocyclyl,substituted aryl, or substituted heteroaryl.
 19. The compound of any oneof claims 1-14, wherein at least R^(55a) or R^(55b) is other thanhydrogen.
 20. The compound of any one of claims 1-14, wherein R^(55a)and R^(55b) is each independently hydrogen, substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, substituted orunsubstituted alkynyl,

wherein: each instance of R^(a) is independently hydrogen, halogen,—NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),—C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4), —N(R^(D4))C(═O)R^(D4),—OC(═O)N(R^(D4))₂, —N(R^(D4))C(═O)OR^(D4), S(═O)₂R^(D4), —S(═O)₂OR^(D4),—OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or —N(R^(D4))S(═O)₂R^(D4),substituted or unsubstituted C₁₋₆ alkyl, substituted or unsubstitutedC₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted orunsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted 3- to6-membered heterocyclyl, substituted or unsubstituted C₅₋₁₀ aryl,substituted or unsubstituted 5- to 10-membered heteroaryl; each instanceof R^(D4) is independently hydrogen, substituted or unsubstituted C₁₋₆alkyl, substituted or unsubstituted C₂₋₆ alkenyl, substituted orunsubstituted C₂₋₆ alkynyl, substituted or unsubstituted C₃₋₆carbocyclyl, substituted or unsubstituted 3- to 6-membered heterocyclyl,substituted or unsubstituted C₅₋₁₀ aryl, substituted or unsubstituted 5-to 10-membered heteroaryl, an oxygen protecting group when attached tooxygen, a nitrogen protecting group when attached to nitrogen, or twoR^(D4) groups are taken with the intervening atoms to form a substitutedor unsubstituted heterocyclic ring; and p is an integer selected from 0to
 11. 21. The compound of any one of claims 1-14, wherein R^(55a) andR^(55b) is each independently hydrogen, substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, substituted orunsubstituted alkynyl,

wherein: each instance of R^(a) is independently hydrogen, halogen,—NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),—C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4), —N(R^(D4))C(═O)R^(D4),—OC(═O)N(R^(D4))₂, —N(R^(D4))C(═O)OR^(D4), —S(═O)₂R^(D4),—S(═O)₂OR^(D4), —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or—N(R^(D4))S(═O)₂R^(D4), substituted or unsubstituted C₁₋₆ alkyl,substituted or unsubstituted C₂₋₆ alkenyl, substituted or unsubstitutedC₂₋₆ alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl, substitutedor unsubstituted 3- to 6-membered heterocyclyl, substituted orunsubstituted C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-memberedheteroaryl; each instance of R^(D4) is independently hydrogen,substituted or unsubstituted C₁₋₆ alkyl, substituted or unsubstitutedC₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted orunsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted 3- to6-membered heterocyclyl, substituted or unsubstituted C₅₋₁₀ aryl,substituted or unsubstituted 5- to 10-membered heteroaryl, an oxygenprotecting group when attached to oxygen, a nitrogen protecting groupwhen attached to nitrogen, or two R^(D4) groups are taken with theintervening atoms to form a substituted or unsubstituted heterocyclicring; and p is an integer selected from 0 to
 3. 22. The compound of anyone of claims 1-14, wherein R^(55a) and R^(55b) is each independentlyhydrogen, substituted or unsubstituted alkyl,

wherein: each instance of R^(a) is independently hydrogen, halogen,—NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),—C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4), —N(R^(D4))C(═O)R^(D4),—OC(═O)N(R^(D4))₂, —N(R^(D4))C(═O)OR^(D4), —S(═O)₂R^(D4),—S(═O)₂OR^(D4), —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or—N(R^(D4))S(═O)₂R^(D4), substituted or unsubstituted C₁₋₆ alkyl,substituted or unsubstituted C₂₋₆ alkenyl, substituted or unsubstitutedC₂₋₆ alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl, substitutedor unsubstituted 3- to 6-membered heterocyclyl, substituted orunsubstituted C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-memberedheteroaryl; each instance of R^(D4) is independently hydrogen,substituted or unsubstituted C₁₋₆ alkyl, substituted or unsubstitutedC₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted orunsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted 3- to6-membered heterocyclyl, substituted or unsubstituted C₅₋₁₀ aryl,substituted or unsubstituted 5- to 10-membered heteroaryl, an oxygenprotecting group when attached to oxygen, a nitrogen protecting groupwhen attached to nitrogen, or two R^(D4) groups are taken with theintervening atoms to form a substituted or unsubstituted heterocyclicring; and p is an integer selected from 0 to
 5. 23. The compound of anyone of claims 1-14, wherein R^(55a) and R^(55b) is independentlyhydrogen, substituted or unsubstituted alkyl,

wherein: each instance of R^(a) is independently hydrogen, halogen, —CN,—OR^(D4), —N(R^(D4))₂, -substituted or unsubstituted C₁₋₆ alkyl,substituted or unsubstituted 3- to 6-membered heterocyclyl, substitutedor unsubstituted 5- to 10-membered heteroaryl; each instance of R^(D4)is independently hydrogen or substituted or unsubstituted C₁₋₆ alkyl;and p is an integer selected from 0 to
 2. 24. The compound of any one ofclaims 1-14, wherein R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl.
 25. The compound of any one ofclaims 1-14, wherein R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl selected from the groupconsisting of:

wherein: each instance of R^(a) is independently hydrogen, oxo, halogen,—NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),—C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4), —N(R^(D4))C(═O)R^(D4),—OC(═O)N(R^(D4))₂, —N(R^(D4))C(═O)OR^(D4), —S(═O)₂R^(D4),—S(═O)₂OR^(D4), —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or—N(R^(D4))S(═O)₂R^(D4), substituted or unsubstituted C₁₋₆ alkyl,substituted or unsubstituted C₂₋₆ alkenyl, substituted or unsubstitutedC₂₋₆ alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl, substitutedor unsubstituted 3- to 6-membered heterocyclyl, substituted orunsubstituted C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-memberedheteroaryl; each instance of R^(D4) is independently hydrogen,substituted or unsubstituted C₁₋₆ alkyl, substituted or unsubstitutedC₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted orunsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted 3- to6-membered heterocyclyl, substituted or unsubstituted C₅₋₁₀ aryl,substituted or unsubstituted 5- to 10-membered heteroaryl, an oxygenprotecting group when attached to oxygen, a nitrogen protecting groupwhen attached to nitrogen, or two R^(D4) groups are taken with theintervening atoms to form a substituted or unsubstituted heterocyclicring; and p is an integer selected from 0 to
 10. 26. The compound of anyone of claims 1-14, wherein R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl selected from the groupconsisting of:

wherein: each instance of R^(a) is independently hydrogen, oxo, halogen,—NO₂, —CN, —OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4),—C(═O)N(R^(D4))₂, —OC(═O)R^(D4), —OC(═O)OR^(D4), —N(R^(D4))C(═O)R^(D4),—OC(═O)N(R^(D4))₂, —N(R^(D4))C(═O)OR^(D4), —S(═O)₂R^(D4),—S(═O)₂OR^(D4), —OS(═O)₂R^(D4), —S(═O)₂N(R^(D4))₂, or—N(R^(D4))S(═O)₂R^(D4), substituted or unsubstituted C₁₋₆ alkyl,substituted or unsubstituted C₂₋₆ alkenyl, substituted or unsubstitutedC₂₋₆ alkynyl, substituted or unsubstituted C₃₋₆ carbocyclyl, substitutedor unsubstituted 3- to 6-membered heterocyclyl, substituted orunsubstituted C₅₋₁₀ aryl, substituted or unsubstituted 5- to 10-memberedheteroaryl; each instance of R^(D4) is independently hydrogen,substituted or unsubstituted C₁₋₆ alkyl, substituted or unsubstitutedC₂₋₆ alkenyl, substituted or unsubstituted C₂₋₆ alkynyl, substituted orunsubstituted C₃₋₆ carbocyclyl, substituted or unsubstituted 3- to6-membered heterocyclyl, substituted or unsubstituted C₅₋₁₀ aryl,substituted or unsubstituted 5- to 10-membered heteroaryl, an oxygenprotecting group when attached to oxygen, a nitrogen protecting groupwhen attached to nitrogen, or two R^(D4) groups are taken with theintervening atoms to form a substituted or unsubstituted heterocyclicring; and p is an integer selected from 0 to
 10. 27. The compound of anyone of claims 1-14, wherein R^(55a) and R^(55b) join together with theintervening atoms to form a substituted or unsubstituted heterocyclyl orsubstituted or unsubstituted heteroaryl selected from the groupconsisting of:

wherein: each instance of R^(a) is independently hydrogen, oxo, halogen,—CN, —OR^(D4), —N(R^(D4))₂, or substituted or unsubstituted C₁₋₆ alkyl;each instance of R^(D4) is independently hydrogen or substituted orunsubstituted C₁₋₆ alkyl; and p is an integer selected from 0 to
 2. 28.The compound of any one of claims 1-11, wherein X is R^(55c).
 29. Thecompound of any one of claims 1-11 and 28, wherein R^(55c) issubstituted or unsubstituted phenyl or carbon-bound substituted orunsubstituted heteroaryl containing at least one nitrogen in theheteroaryl ring.
 30. The compound of any one of claims 1-11 and 28-29,wherein R^(55c) is substituted or unsubstituted phenyl or carbon-boundsubstituted or unsubstituted heteroaryl selected from the groupconsisting of pyridyl, isothiazolyl, thiazolyl, pyrimidyl, pyrazinyl,and oxazolyl.
 31. The compound of any one of claims 1-11 and 28-30,wherein R^(55c) is selected from the group consisting of:

wherein: each instance of R^(a) is independently hydrogen, halogen, —CN,—OR^(D4), —N(R^(D4))₂, —C(═O)R^(D4), —C(═O)OR^(D4), or substituted orunsubstituted C₁₋₆ alkyl; each instance of R^(D4) is independentlyhydrogen or substituted or unsubstituted C₁₋₆ alkyl; and p is an integerselected from 0 to
 2. 32. The compound of any one of claims 1-31,wherein R⁵ is hydrogen in the cis position, relative to R¹⁹.
 33. Thecompound of any one of claims 1-31, wherein R⁵ is hydrogen in the transposition, relative to R¹⁹.
 34. The compound of any one of claims 1-31,wherein R⁵ is methyl in the cis position, relative to R¹⁹.
 35. Thecompound of any one of claims 1-31, wherein R⁵ is methyl in the transposition, relative to R¹⁹.
 36. The compound of claim 1, wherein thecompound is a compound of Formula (I-b), (I-c), (I-d), (I-e), (I-l),(I-m), (I-n), or (I-p):

or a pharmaceutically acceptable salt thereof.
 37. The compound of claim1, wherein the compound is a compound of Formula (I-f), (I-g), or (I-h):

or a pharmaceutically acceptable salt thereof.
 38. The compound of claim1, wherein the compound is a compound of Formula (I-i), (I-j), or (I-k):

or a pharmaceutically acceptable salt thereof.
 39. The compound of claim1, wherein the compound is a compound of Formula (I-o):

or a pharmaceutically acceptable salt thereof.
 40. The compound of claim1, wherein the compound is a compound of Formula (I-qq), (I-q), (I-s),(I-t), or (I-u):

or a pharmaceutically acceptable salt thereof, wherein Q, Q′, and Q″ areeach independently CR^(w) or N; R^(w) is hydrogen, cyano, —NH₂, orsubstituted or unsubstituted alkyl; and at least one of Q, Q′, and Q″ isCR^(w).
 41. The compound of claim 1, wherein the compound is a compoundof Formula (I-r):

or a pharmaceutically acceptable salt thereof, wherein k is an integer 1or 2; R^(z) is substituted or unsubstituted alkyl or substituted orunsubstituted aryl; or two R^(z)s on adjacent carbons combine with theintervening atoms to form a substituted or unsubstituted aryl; and j isan integer 0-6.
 42. The compound of claim 1, wherein the compound is acompound of Formula (I-v), (I-w), or (I-x):

or a pharmaceutically acceptable salt thereof.
 43. The compound of claim42, wherein R^(55c) is substituted or unsubstituted phenyl orcarbon-bound substituted or unsubstituted heteroaryl containing at leastone nitrogen in the heteroaryl ring.
 44. The compound of claim 42 or 43,wherein R^(55c) is substituted or unsubstituted phenyl or carbon-boundsubstituted or unsubstituted heteroaryl selected from the groupconsisting of: pyridyl, isothiazolyl, thiazolyl, pyrimidyl, pyrazinyl,and oxazolyl.
 45. The compound of any one of claims 1-44, wherein R¹⁹ ishydrogen or substituted or unsubstituted alkyl.
 46. The compound of anyone of claims 1-45, wherein R¹⁹ is hydrogen or substituted C₁₋₆alkyl.47. The compound of any one of claims 1-46, wherein R¹⁹ is —CH₂OCH₃. 48.The compound of any one of claims 1-45, wherein R¹⁹ is hydrogen orunsubstituted C₁₋₆alkyl.
 49. The compound of any one of claims 1-45 and48, wherein R¹⁹ is unsubstituted C₁₋₆alkyl.
 50. The compound of any oneof claims 1-45 and 48-49, wherein R¹⁹ is methyl.
 51. The compound of anyone of claims 1-45 and 48-49, wherein R¹⁹ is ethyl.
 52. The compound ofany one of claims 1-51, wherein R¹⁸ is unsubstituted alkyl.
 53. Thecompound of any one of claims 1-52, wherein R¹⁸ is unsubstitutedC₁₋₆alkyl.
 54. The compound of any one of claims 1-53, wherein R¹⁸ ismethyl.
 55. The compound of any one of claims 1-53, wherein R¹⁸ isethyl.
 56. The compound of any one of claims 1-51, wherein R¹⁸ issubstituted alkyl.
 57. The compound of any one of claims 1-56, whereinR^(D) is each independently hydrogen, halogen, —CN, —NO₂, oxo, hydroxy,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, substituted or unsubstituted alkynyl, or substituted orunsubstituted carbocyclyl.
 58. The compound of any one of claims 1-57,wherein R^(D) is each independently hydrogen, oxo, substituted orunsubstituted alkyl, hydroxy, or substituted or unsubstitutedcarbocyclyl.
 59. The compound of any one of claims 1-58, wherein R^(D)is independently hydrogen, oxo, methyl, ethyl, hydroxy, or cyclopropyl.60. The compound of claim 1, wherein the compound is a compound ofFormula (I-Ib), (I-Ic), (I-Id), (I-Ie), (I-Il), (I-Im), (I-In), (I-Ip1),or (I-Ip2):

or a pharmaceutically acceptable salt thereof.
 61. The compound of claim1, wherein the compound is a compound of Formula (I-If), (I-Ig), or(I-Ih):

or a pharmaceutically acceptable salt thereof.
 62. The compound of claim1, wherein the compound is a compound of Formula (I-Ii), (I-Ij), or(I-Ik):

or a pharmaceutically acceptable salt thereof.
 63. The compound of claim1, wherein the compound is a compound of Formula (I-Io1) or (I-Io2):

or a pharmaceutically acceptable salt thereof.
 64. The compound of claim1, wherein the compound is a compound of Formula (I-Iqq), (I-Iq1),(I-Iq2), (I-It1), (I-It2), (I-Iu1), or (I-Iu2):

or a pharmaceutically acceptable salt thereof, wherein Q, Q′, and Q″ areeach independently CR^(w) or N; R^(w) is hydrogen, cyano, —NH₂, orsubstituted or unsubstituted alkyl; and at least one of Q, Q′, and Q″ isCR^(w).
 65. The compound of claim 64, wherein R¹⁹ is hydrogen, methyl,ethyl, or methoxymethyl.
 66. The compound of claim 1, wherein thecompound is a compound of Formula (I-Irr), (I-Ir1) or (I-Ir2):

or a pharmaceutically acceptable salt thereof, wherein k is an integer 1or 2; R^(z) is substituted or unsubstituted alkyl or substituted orunsubstituted aryl; or two R^(z)s on adjacent carbons combine with theintervening atoms to form a substituted or unsubstituted aryl; and j aninteger 0-6.
 67. The compound of claim 66, wherein the compound is acompound of Formula (I-Ir3) or (I-Ir4):

or a pharmaceutically acceptable salt thereof, wherein k is an integer 1or 2; R^(z′) is substituted or unsubstituted alkyl or cyano; and j′ aninteger 0-4.
 68. The compound of claim 1, wherein the compound is acompound of Formula (I-Iw1), (I-Iw2), (I-Ix1), or (I-Ix2):

or a pharmaceutically acceptable salt thereof.
 69. The compound of anyone of claims 1-68, wherein R^(3a) is substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, or substituted orunsubstituted alkynyl.
 70. The compound of any one of claims 1-69,wherein R^(3a) is substituted or unsubstituted C₁₋₆ alkyl.
 71. Thecompound of any one of claims 1-70, wherein R^(3a) is substituted C₁₋₆alkyl.
 72. The compound of any one of claims 1-70, wherein R^(3a) isunsubstituted C₁₋₆ alkyl.
 73. The compound of any one of claims 1-70 and72, wherein R^(3a) is methyl, ethyl, n-propyl, iso-propyl, n-butyl,iso-butyl.
 74. The compound of any one of claims 1-70 and 72-73, whereinR^(3a) is methyl.
 75. The compound of any one of claims 1-71, whereinR^(3a) is —CH₂OCH₃ or CH₂OCH₂CH₃.
 76. The compound of any one of claims1-71, wherein R^(3a) is —CH₂C₃H₅.
 77. The compound of any one of claims1-68, wherein R^(3a) is substituted or unsubstituted alkyl orsubstituted or unsubstituted carbocyclyl.
 78. The compound of any one ofclaims 1-68, wherein R^(3a) is substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, substituted or unsubstitutedaryl, or substituted or unsubstituted heteroaryl.
 79. The compound ofany one of claims 1-68 and 77-78, wherein R^(3a) is substituted orunsubstituted carbocyclyl.
 80. The compound of any one of claims 1-68and 77-79, wherein R^(3a) is cyclopropyl.
 81. The compound of any one ofclaims 1-80, wherein R^(4a) and R^(4b) is each independently hydrogen,halogen, —CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 82. The compound of any one of claims 1-80, whereinR^(4a) and R^(4b) is each independently hydrogen, halogen, —CN, —NO₂,—OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or —N(R^(F6))C(═O)R^(F6); whereineach instance of R^(F6) is independently hydrogen, substituted orunsubstituted alkyl, substituted or unsubstituted alkenyl, substitutedor unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, or two R^(F6) groups aretaken with the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 83. The compound of any one of claims 1-80, whereinR^(4a) and R^(4b) are independently hydrogen, —OH, or substituted orunsubstituted C₁₋₆ alkyl.
 84. The compound of any one of claims 1-80,wherein each of R^(4a) and R^(4b) are independently hydrogen, —OH, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆ alkoxyhalo.
 85. The compoundof any one of claims 1-80, wherein R^(4a) and R^(4b) are independently—CH₃, —CH₂CH₃, —OH, —OCH₃, or —CH(CH₃)₂.
 86. The compound of any one ofclaims 1-80, wherein R^(4a) and R^(4b) are both hydrogen.
 87. Thecompound of any one of claims 1-80, wherein R^(4a) and R^(4b) are joinedto form an oxo (═O) group.
 88. The compound of any one of claims 1-87,wherein R^(6a) and R^(6b) is independently hydrogen, halogen,substituted or unsubstituted alkyl, substituted or unsubstitutedalkenyl, or substituted or unsubstituted alkynyl.
 89. The compound ofany one of claims 1-87, wherein R^(6a) and R^(6b) is independentlyhydrogen or substituted alkyl.
 90. The compound of any one of claims1-87, wherein R^(6a) and R^(6b) is independently hydrogen orunsubstituted alkyl.
 91. The compound of any one of claims 1-87, whereinboth R^(6a) and R^(6b) are hydrogen.
 92. The compound of any one ofclaims 1-87, wherein R^(6a) is halogen or alkyl and R^(6b) is hydrogen.93. The compound of any one of claims 1-87, wherein R^(6a) and R^(6b)are both halogen.
 94. The compound of any one of claims 1-87, whereinR^(6a) and R^(6b) are both alkyl.
 95. The compound of any one of claims1-94, wherein R^(7a) and R^(7b) is each independently hydrogen, halogen,—CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 96. The compound of any one of claims 1-94, whereinR^(7a) and R^(7b) is each independently hydrogen, halogen, —CN, —NO₂,—OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or —N(R^(F6))C(═O)R^(F6); whereineach instance of R^(F6) is independently hydrogen, substituted orunsubstituted alkyl, substituted or unsubstituted alkenyl, substitutedor unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, or two R^(F6) groups aretaken with the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 97. The compound of any one of claims 1-94, whereinR^(7a) and R^(7b) are independently hydrogen, —OH, or substituted orunsubstituted C₁₋₆ alkyl.
 98. The compound of any one of claims 1-94,wherein each of R^(7a) and R^(7b) are independently hydrogen, —OH, C₁₋₆alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆ alkoxyhalo.
 99. The compoundof any one of claims 1-94, wherein R^(7a) and R^(7b) are independently—CH₃, —CH₂CH₃, —OH, —OCH₃, or —CH(CH₃)₂.
 100. The compound of any one ofclaims 1-94, wherein R^(7a) and R^(7b) are both hydrogen.
 101. Thecompound of any one of claims 1-94, wherein R^(7a) and R^(7b) are joinedto form an oxo (═O) group.
 102. The compound of any one of claims 1-101,wherein R^(11a) and R^(11b) is each independently hydrogen, halogen,—CN, —NO₂, substituted or unsubstituted alkyl, substituted orunsubstituted alkenyl, substituted or unsubstituted alkynyl, —OR^(E5),—OC(═O)R^(E5), —OS(═O)₂OR^(E5), —N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5),—N(R^(E5))S(═O)₂R^(E5), —N(R^(E5))S(═O)₂OR^(E5); wherein each instanceof R^(E5) is independently hydrogen, substituted or unsubstituted alkyl,substituted or unsubstituted alkenyl, substituted or unsubstitutedalkynyl, substituted or unsubstituted carbocyclyl, substituted orunsubstituted heterocyclyl, substituted or unsubstituted aryl, orsubstituted or unsubstituted heteroaryl, or two R^(E5) groups are takenwith the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 103. The compound of any one of claims 1-101, whereinR^(11a) and R^(11b) is each independently hydrogen, halogen, —CN, —NO₂,—OR^(F6), —OC(═O)R^(F6), —N(R^(F6))₂, or —N(R^(F6))C(═O)R^(F6); whereineach instance of R^(F6) is independently hydrogen, substituted orunsubstituted alkyl, substituted or unsubstituted alkenyl, substitutedor unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, or two R^(F6) groups aretaken with the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 104. The compound of any one of claims 1-101, whereinR^(11a) and R^(11b) are independently hydrogen, —OH, or substituted orunsubstituted C₁₋₆ alkyl.
 105. The compound of any one of claims 1-101,wherein each of R^(11a) and R^(11b) are independently hydrogen, —OH,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆ alkoxyhalo.
 106. Thecompound of any one of claims 1-101, wherein R^(11a) and R^(11b) areindependently —CH₃, —CH₂CH₃, —OH, —OCH₃, or —CH(CH₃)₂.
 107. The compoundof any one of claims 1-101, wherein R^(11a) and R^(11b) are bothhydrogen.
 108. The compound of any one of claims 1-101, wherein R^(11a)and R^(11b) are joined to form an oxo (═O) group.
 109. The compound ofany one of claims 1-108, wherein R^(12a) and R^(12b) is eachindependently hydrogen, halogen, —CN, —NO₂, substituted or unsubstitutedalkyl, substituted or unsubstituted alkenyl, substituted orunsubstituted alkynyl, —OR^(E5), —OC(═O)R^(E5), —OS(═O)₂OR^(E5),—N(R^(E5))₂, or —N(R^(E5))C(═O)R^(E5), —N(R^(E5))S(═O)₂R^(E5),—N(R^(E5))S(═O)₂OR^(E5); wherein each instance of R^(E5) isindependently hydrogen, substituted or unsubstituted alkyl, substitutedor unsubstituted alkenyl, substituted or unsubstituted alkynyl,substituted or unsubstituted carbocyclyl, substituted or unsubstitutedheterocyclyl, substituted or unsubstituted aryl, or substituted orunsubstituted heteroaryl, or two R^(E5) groups are taken with theintervening atoms to form a substituted or unsubstituted heterocyclicring.
 110. The compound of any one of claims 1-108, wherein R^(12a) andR^(12b) is each independently hydrogen, halogen, —CN, —NO₂, —OR^(F6),—OC(═O)R^(F6), —N(R^(F6))₂, or —N(R^(F6))C(═O)R^(F6); wherein eachinstance of R^(F6) is independently hydrogen, substituted orunsubstituted alkyl, substituted or unsubstituted alkenyl, substitutedor unsubstituted alkynyl, substituted or unsubstituted carbocyclyl,substituted or unsubstituted heterocyclyl, or two R^(F6) groups aretaken with the intervening atoms to form a substituted or unsubstitutedheterocyclic ring.
 111. The compound of any one of claims 1-108, whereinR^(12a) and R^(12b) are independently hydrogen, —OH, or substituted orunsubstituted C₁₋₆ alkyl.
 112. The compound of any one of claims 1-108,wherein each of R^(12a) and R^(12b) are independently hydrogen, —OH,C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆ alkoxy, or C₁₋₆ alkoxyhalo.
 113. Thecompound of any one of claims 1-108, wherein R^(12a) and R^(12b) areindependently —CH₃, —CH₂CH₃, —OH, —OCH₃, or —CH(CH₃)₂.
 114. The compoundof any one of claims 1-108, wherein R^(12a) and R^(12b) are bothhydrogen.
 115. The compound of any one of claims 1-108, wherein R^(12a)and R^(12b) are joined to form an oxo (═O) group.
 116. The compound ofany one of claims 1-115, wherein R^(1a) and R^(1b) are independentlyhydrogen, —OH, or substituted or unsubstituted C₁₋₆ alkyl.
 117. Thecompound of any one of claims 1-115, wherein each of R^(1a) and R^(1b)are independently hydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆alkoxy, or C₁₋₆ alkoxyhalo.
 118. The compound of any one of claims1-117, wherein R^(1a) and R^(1b) are both hydrogen.
 119. The compound ofany one of claims 1-118, wherein R^(2a) and R^(2b) are independentlyhydrogen, —OH, or substituted or unsubstituted C₁₋₆ alkyl.
 120. Thecompound of any one of claims 1-118, wherein each of R^(2a) and R^(2b)are independently hydrogen, —OH, C₁₋₆ alkyl, C₁₋₆ haloalkyl, C₁₋₆alkoxy, or C₁₋₆ alkoxyhalo.
 121. The compound of any one of claims1-120, wherein R^(2a) and R^(2b) are both hydrogen.
 122. Apharmaceutical composition comprising a compound of any one of claims1-121 or a pharmaceutically acceptable salt thereof, and apharmaceutically acceptable excipient.
 123. A method of treating aCNS-related disorder in a subject in need thereof, comprisingadministering to the subject an effective amount of a compound of anyone of claims 1-121 or a pharmaceutically acceptable salt thereof. 124.The method of claim 123, wherein the CNS-related disorder is a sleepdisorder, a mood disorder, a schizophrenia spectrum disorder, aconvulsive disorder, a disorder of memory and/or cognition, a movementdisorder, a personality disorder, autism spectrum disorder, pain,traumatic brain injury, a vascular disease, a substance abuse disorderand/or withdrawal syndrome, tinnitus, or status epilepticus.
 125. Themethod of claim 123, wherein the CNS-related disorder is depression.126. The method of claim 123, wherein the CNS-related disorder ispostpartum depression.
 127. The method of claim 123, wherein theCNS-related disorder is major depressive disorder.
 128. The method ofclaim 127, wherein the major depressive disorder is moderate majordepressive disorder.
 129. The method of claim 127, wherein the majordepressive disorder is severe major depressive disorder.